An animal bed device based on a cryogenic coil for brain 129 Xe MRI

Abstract

The present invention discloses an animal bed device based on a cryogenic coil for <supgt;129< / supgt;Xe MRI of the brain. The present invention adjusts in various ways through front and rear positioning rods, a lifting table, and fine-tuning long bolts to make the brain of the experimental animal closely adhere to the cryogenic coil, ensuring a high filling factor of the coil, and thus ensuring a high signal-to-noise ratio of the imaging. The present invention is also provided with a simulation chamber, which restores the relative positions of the cryogenic coil and the animal bed in the cavity of the magnetic resonance scanner in a 1:1 manner, and ensures that the brain of the experimental animal closely adheres to the cryogenic coil when the animal bed enters the cavity of the magnetic resonance scanner without using the conventional laser positioning method. The cryogenic coil simulation component and the cryogenic coil housing simulation part are made of transparent materials, facilitating the observation of the fitting degree between the experimental animal and the cryogenic coil simulation component and adjustment; the lifting table of the present invention also provides a flat surface to support the brain of the animal, preventing the animal from being unable to be properly positioned due to interference from the tracheal intubation during animal positioning.

Description

Technical Field

[0001] The present invention belongs to the field of magnetic resonance imaging technology, and specifically relates to a method for using a cryo coil for brain imaging. 129 Xe MRI animal bed apparatus for brain hyperpolarization 129 Magnetic resonance imaging of Xe gas. Background Art

[0002] Magnetic resonance imaging (MRI) is a non-ionizing radiation and non-radioactive imaging method with the advantages of high soft tissue contrast and multi-parameter imaging. Therefore, it has been widely used in clinical practice. 129 Xe gas MRI is a rapidly developing imaging method with great potential. 129 The spin polarization of Xe nuclei is increased by 4-5 orders of magnitude compared to that under thermal equilibrium conditions, achieving hyperpolarization. 129 Xe gas. Hyperpolarization 129 Xe is non-toxic and can dissolve in lung tissue and blood after being inhaled into the lungs, and enter the brain through the bloodstream. 129 Xe MRI has no background signal interference, so it is very suitable for brain perfusion imaging, hemodynamic assessment, etc. 129 Xe signal is only in the alveoli 129 1 to 2% of the Xe signal greatly limits the brain 129 Signal-to-noise ratio of Xe MRI.

[0003] MRI cryocoils use cryogens to cool the RF coils and preamplifiers, reducing the thermal noise of the RF coils and related electronic components, which can significantly improve the imaging signal-to-noise ratio. Compared with standard room-temperature RF coils, cryocoils can increase the sensitivity of in vivo detection by 2-5 times, and can achieve higher spatial resolution and shorter scanning time. Combining hyperpolarized gas imaging technology with cryocoils is expected to improve brain 129 However, there is currently no cryo-coil for live animal brain imaging. 129 The reason for the research on Xe imaging is that there is currently no 129 Xe MRI animal bed adapted for commercial MRI cryo coils.

[0004] Unlike the normal temperature brain coil that is installed on the bed and enters the MRI cavity with the animal, the commercial MRI cryo coil is fixed in the MRI cavity and cannot be moved during use. The animal needs to be placed under the cryo coil in a prone position using a specially adapted cryo coil bed. 1H) For MRI, only a breathing mask is usually integrated into the bed to achieve gas anesthesia for animals in the experiment. 129 In Xe MRI experiments, it is necessary to transmit hyperpolarization into the lungs of animals. 129 To deliver Xe gas, the animal needs to undergo endotracheal intubation and be connected to the gas delivery system integrated into the bed in a lying position. Existing commercial prone cryocoil beds cannot achieve the above functions. Summary of the invention

[0005] The purpose of the present invention is to provide a method for treating the above-mentioned problems in the prior art based on a cryo coil for brain 129 Animal bed setup for Xe MRI.

[0006] The above-mentioned purpose of the present invention is achieved by the following technical means:

[0007] A cryocoil-based method for the brain 129 The animal bed device of Xe MRI comprises an animal bed, the front part of the animal bed is an animal placement area, the top surface of the animal bed is a plane, the end cross section of the animal placement area is a semicircular arc, the top plane of the animal placement area is provided with an animal placement slot along the length direction of the animal bed, the two sides of the animal placement slot are side guards, the side guards are provided with long limiting holes along the length direction of the animal bed, the limiting holes penetrate the front part of the side guards, and front and rear positioning rods are slidably provided in the limiting holes; the animal placement slot is also provided with an installation slot, a lifting platform is provided in the installation slot, and a slot along the width direction of the animal bed is also provided The lifting platform is provided with a central rod, and both ends of the central rod are fixedly connected to the groove wall of the mounting groove, and the fixing part at the rear end of the lifting platform is hinged to the central rod; an insertion opening is provided on the lifting platform, and a support rod mounting groove is provided at the bottom of the lifting platform, the support rod mounting groove is perpendicular to the central rod, a support rod is arranged in the support rod mounting groove, the fixed end of the support rod is hinged to the support rod mounting groove, and the other end of the support rod is a rotating end; a first threaded hole that passes through the animal bed is provided at the center of the groove bottom of the animal placement groove, and a thread-matched fine-tuning long bolt is inserted into the first threaded hole, and the bottom of the fine-tuning long bolt extends out of the bottom of the animal placement area.

[0008] As described above, the mounting groove is also provided with a plurality of limiting grooves arranged side by side. The rotating end of the support rod is placed in the limiting groove to fix the lifting angle of the lifting platform; the rotating end of the support rod is placed in different limiting grooves to adjust the lifting platform to different lifting angles.

[0009] As described above, a plurality of positioning holes are evenly distributed along the length direction of the animal bed and communicated with the limit holes on the top of the side block body, and positioning holes are also formed on the front and rear positioning rods. The latch is inserted into the positioning holes of the front and rear positioning rods and one of the positioning holes of the side block body.

[0010] As mentioned above, the bottom of the animal placement area is a transparent bottom cover, the top of the transparent bottom cover is a plane, and the top of the transparent bottom cover is a hose placement plane. A plurality of hose slots along the length direction of the animal bed are arranged side by side on the hose placement plane, and a five-way connector is arranged in front of the hose slot. The five-way connector is placed in a connector placement slot provided on the animal bed. The transparent bottom cover is fixedly connected to the hose placement plane, and the bottom of the fine-tuning long bolt extends out of the transparent bottom cover; an animal fixing cover is also arranged above the animal placement slot, and the animal fixing cover is fixedly connected to the top of the side guard body.

[0011] As mentioned above, the rear part of the animal bed is a gas storage area, and the end cross-section of the gas storage area is semicircular. The gas storage area is provided with an oxygen gas pipeline, a xenon gas pipeline, an exhalation gas pipeline, and a sensor gas pipeline. The cross-section of the hose slot is arched, and each gas pipeline is clamped in a hose slot. The five-way connector includes a cannula interface and four gas pipeline interfaces. The four gas pipeline interfaces of the five-way connector are respectively connected to the outlet end of the oxygen gas pipeline, the outlet end of the xenon gas pipeline, the inlet end of the exhalation gas pipeline, and the inlet end of the sensor gas pipeline. The cannula interface is located at the top of the five-way connector, one end of the cannula connecting tube is connected to the cannula interface of the five-way connector, and the other end of the cannula connecting tube passes through the cannula opening of the lifting platform and is connected to the tracheal cannula through a Luer lock joint.

[0012] As mentioned above, a pressure-stabilizing tank is also placed on the top plane of the gas storage area, and a Tedlar sampling bag filled with hyperpolarized gas is arranged in the pressure-stabilizing tank. A plurality of pipeline perforations are arranged on the animal bed. The air inlet end of the xenon gas pipeline passes through the pipeline perforations and is connected to the air outlet end of the first pneumatic valve. The air inlet end of the first pneumatic valve is connected to the pressure-stabilizing tank through a Luer lock joint; the air outlet end of the exhalation gas pipeline is connected to the air inlet end of the second pneumatic valve, and the air outlet end of the second pneumatic valve is connected to the outside; the air inlet end of the oxygen gas pipeline passes through the pipeline perforations and extends to the outside of the animal bed to connect to the oxygen The first pneumatic valve and the second pneumatic valve are connected to the gas storage cylinder; the air inlet end of the sensor air path pipeline is also connected to the oxygen air path pipeline, the xenon air path pipeline, and the exhalation air path pipeline; the control ends of the first pneumatic valve and the second pneumatic valve are respectively connected to the air outlet end of a nitrogen air path pipeline, the air inlet end of the oxygen air path pipeline, the air inlet ends of the two nitrogen air path pipelines, and the other end of the sensor air path pipeline are stored and sorted by a cable tie, and the cable tie is arranged behind the pressure-stabilizing tank; the pressure-stabilizing tank, the first pneumatic valve, the second pneumatic valve, and the cable tie are all fixedly arranged at the rear of the animal bed.

[0013] As mentioned above, the radius of the end of the gas storage area is greater than the radius of the end of the animal placement area, and a placement plane is provided at the bottom of the gas storage area.

[0014] As mentioned above, a tail motor fixing slot is also provided at the bottom of the end of the animal bed.

[0015] It also includes a simulation chamber, which includes a low-temperature coil simulation component, a low-temperature coil shell simulation component, an L-shaped bottom support platform, and a central tube simulation component. The low-temperature coil simulation component and the low-temperature coil shell simulation component are both transparent. The low-temperature coil simulation component and the low-temperature coil shell simulation component are fixed on the vertical plate of the bottom support platform; the central tube simulation component is fixed on the horizontal plate of the bottom support platform, the animal bed is placed in the central tube simulation component, and the front of the animal placement area extends into the low-temperature coil shell simulation component.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The present invention uses a plurality of methods such as front and rear positioning rods, a lifting platform, and fine-tuning long bolts to adjust the experimental animal's brain to be close to the low-temperature coil, thereby ensuring a high filling factor of the coil and thus ensuring a high signal-to-noise ratio for imaging.

[0018] (2) The simulation chamber of the present invention restores the relative positions of the cryocoil and the animal bed in the cavity of the magnetic resonance scanner in a 1:1 manner, and ensures that the brain of the experimental animal is in close contact with the cryocoil when the animal bed enters the cavity of the magnetic resonance scanner without using conventional laser positioning. The cryocoil simulation component and the cryocoil shell simulation component are made of transparent materials, which facilitates observation and adjustment of the degree of fit between the experimental animal and the cryocoil simulation component.

[0019] (3) The hose clamping slot of the present invention can cooperate with the animal tracheal intubation to achieve low polarization loss transmission of hyperpolarized gas to the lungs, avoiding mechanical interference with the cryogenic coil.

[0020] (4) The animal bed device of the present invention is designed with an animal fixing cover, which is fixed to the animal bed by screws to prevent the animal from falling out of the animal chamber after waking up due to poor anesthesia during the experiment.

[0021] (5) The lifting platform of the present invention not only lifts the animal's brain to bring it close to the cryocoil, but also provides a flat surface to support the animal's brain, thereby preventing the animal from being disturbed by the endotracheal tube when it is positioned; a tube opening is provided at the bottom of the lifting platform to facilitate quick connection with the tube connecting tube after the animal's endotracheal tube is intubated. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0023] Figure 2 is a top view of the animal bed of the present invention (excluding the animal fixing cover);

[0024] Figure 3 It is a schematic diagram of the structure of the animal placement area (excluding the animal fixing cover) of the present invention;

[0025] Figure 4is a top view of the animal bed of the present invention after adding an animal fixing cover;

[0026] Figure 5 is a bottom view of the animal bed of the present invention (excluding the transparent bottom cover);

[0027] Figure 6 is a bottom view of the animal bed of the present invention with a transparent bottom cover added;

[0028] Figure 7 It is a structural schematic diagram of a five-way connector and a lifting platform in an animal display placement area of ​​the present invention;

[0029] Figure 8 It is a schematic diagram of the specific structure of the lifting platform for displaying the animal placement area of ​​the present invention;

[0030] Fig. 9 is a top view of placing an experimental animal on the animal bed of the present invention;

[0031] Reference numerals and corresponding component names:

[0032] 1—animal bed; 2—front and rear positioning rods; 3—lifting platform; 4—fine-tuning long bolts; 5—side stopper; 6—transparent bottom cover; 7—support rod; 8—hose slot; 9—animal fixing cover; 10—limiting groove; 11—pneumatic valve; 12—pressure regulating tank; 13—cable tie; 14—five-way connector; 15—positioning hole; 16—gas pipeline; 17—cryogenic coil simulation component; 18—cryogenic coil shell simulation component; 19—bottom support platform; 20—center tube simulation component; 21—insertion tube opening; 22—tail motor fixing groove; 23—placing plane; 24—center rod; 25—support rod mounting groove. DETAILED DESCRIPTION

[0033] In order to facilitate those skilled in the art to understand and implement the present invention, the present invention is further described in detail below in conjunction with embodiments. The embodiments described herein are only used to illustrate and explain the present invention and are not intended to limit the present invention.

[0034] Example

[0035] A cryocoil-based method for the brain 129The animal bed device of Xe MRI comprises an animal bed 1, the front part of the animal bed 1 is an animal placement area, the top surface of the animal bed 1 is a plane, the end cross section of the animal placement area is a semicircular arc, the top plane of the animal placement area is provided with an animal placement groove along the length direction of the animal bed 1, the width of the animal placement groove is smaller than the width of the animal bed 1, and the two sides of the animal placement groove are side guards 5, the side guards 5 are higher than the animal placement groove, and can prevent the experimental animals placed in the animal placement groove from sliding down from both sides; the side guards 5 are provided with long limiting holes along the length direction of the animal bed 1, the limiting holes penetrate the front part of the side guards 5, and the front and rear positioning rods 2 are slidably arranged in the limiting holes, and the front and rear positioning rods 2 can reciprocate along the limiting hole in the length direction of the side stopper 5. A plurality of positioning holes 15 are evenly distributed along the length direction of the animal bed 1 and communicated with the limiting holes. The front and rear positioning rods 2 are also provided with positioning holes 15. When the front and rear positions of the animal bed 1 need to be adjusted, the front and rear positioning rods 2 are moved to adjust the lengths of the rod sections extending out of the limiting holes on the front and rear positioning rods 2, so that the animal bed 1 is adjusted to the required position. Then, the latch is inserted into the positioning holes 15 of the front and rear positioning rods 2 and one of the positioning holes 15 of the side stopper 5 to fix the positions of the front and rear positioning rods 2 in the limiting holes, thereby fixing the front and rear positions of the animal bed 1.

[0036] The bottom of the animal placement area is a detachable transparent bottom cover 6, the top of the transparent bottom cover 6 is a plane, and the top of the transparent bottom cover 6 is a hose placement plane. The bottom of the fine-tuning long bolt 4 extends out of the transparent bottom cover 6, and a plurality of hose clamping grooves 8 along the length direction of the animal bed 1 are arranged side by side on the hose placement plane. The hose clamping grooves 8 are used to place the air pipeline 16, and the cross-section of the hose clamping grooves 8 is arched, which is convenient for the disassembly and assembly of each air pipeline 16; a five-way connector 14 is arranged in front of the hose clamping groove 8, and the five-way connector 14 is placed in the connector placement groove opened on the animal bed 1, four interfaces of the five-way connector 14 are air pipeline interfaces, and the four air pipeline interfaces correspond to one hose clamping groove 8 respectively, and the last interface is a cannula interface, and the cannula interface is located at the top of the five-way connector 14; the transparent bottom cover 6 is fixedly connected to the hose placement plane by screw holes and screws, and the transparent bottom cover 6 is used to limit the position to prevent the air pipeline 16 installed in the hose clamping groove 8 from falling off from the hose clamping groove 8.

[0037] A first threaded hole penetrating the animal bed 1 is provided at the center of the bottom of the animal placement slot, and a thread-matched fine-tuning long bolt 4 is inserted into the first threaded hole. The bottom of the fine-tuning long bolt 4 extends out of the bottom of the animal placement area. The distance between the bottom of the animal placement area and the working plane in the magnetic resonance scanner can be fine-tuned by adjusting the length of the fine-tuning long bolt 4 extending out of the bottom of the animal bed 1, thereby fine-tuning the distance between the experimental animal in the animal placement slot and the low-temperature coil in the magnetic resonance scanner, and further bringing the experimental animal closer to the low-temperature coil through the lifting platform 3.

[0038] A mounting groove is also provided in the animal placement groove, a lifting platform 3 is provided in the mounting groove, a center rod 24 along the width direction of the animal bed 1 is also provided in the mounting groove, both ends of the center rod 24 are fixedly connected to the groove wall of the mounting groove, the rear part of the lifting platform 3 is a fixed part, and the front part is a rotating part, the fixed part at the rear end of the lifting platform 3 is hinged to the center rod 24, and the lifting platform 3 can be vertically flipped with the center rod 24 as the central axis; a support rod mounting groove 25 is provided at the bottom of the lifting platform 3, the support rod mounting groove 25 is perpendicular to the center rod 24, a support rod 7 is provided in the support rod mounting groove 25, one end of the support rod 7 is a fixed end, and the other end is a rotating end, the fixed end of the support rod 7 is hinged to the support rod mounting groove 25, so that the rotating end of the support rod 7 can rotate with the fixed end of the support rod 7 as the center, and an intubation opening 21 is provided on the lifting platform 3;

[0039] The mounting groove is also provided with a plurality of limiting grooves 10 arranged side by side. By placing the rotating end of the support rod 7 into the limiting groove 10, the lifting angle of the lifting platform 3 (i.e., the angle between the lifting platform 3 and the animal bed 1) can be fixed; by placing the rotating end of the support rod 7 into different limiting grooves 10, the lifting platform 3 can be adjusted to different lifting angles.

[0040] The above-mentioned limiting groove 10 is not limited to a groove, but can also be a limiting protrusion and other components that can fix the rotating end of the support rod 7. If it is set to a limiting protrusion, the adjacent limiting protrusions are spaced at the same interval, so that the rotating end of the support rod 7 can be placed in the interval between adjacent limiting protrusions; the support rod mounting groove 25 at the bottom of the lifting platform can also be set to multiple, each support rod mounting groove 25 is set with a support rod 7, which can better ensure the stability of the lifting platform 3 after flipping. In this embodiment, it is set to two support rod mounting grooves 25 and corresponding support rods 3.

[0041] An animal fixing cover 9 is also provided above the animal placement slot, and the animal fixing cover 9 is fixedly connected to the top of the two side baffles 5 by screw holes and screws. The animal fixing cover 9 is used to prevent the animal from falling off the animal bed 1 after waking up due to poor anesthesia during the experiment.

[0042] The rear part of the animal bed 1 is a gas storage area. The end cross-section of the gas storage area is semicircular. The gas storage area is provided with a plurality of gas pipelines 16, which are oxygen gas pipelines (O 2 Gas pipeline), xenon gas pipeline ( 129Xe gas path pipeline), exhalation gas path pipeline, and sensor gas path pipeline, each gas path pipeline 16 is clamped in a hose clamping slot 8, and the four gas path pipeline interfaces of the five-way connector 14 are respectively connected with the gas outlet end of the oxygen gas path pipeline, the gas outlet end of the xenon gas path pipeline, the gas inlet end of the exhalation gas path pipeline, and the gas inlet end of the sensor gas path pipeline, one end of the cannula connecting tube is connected with the cannula interface of the five-way connector 14, and the other end of the cannula connecting tube passes through the cannula opening 21 of the lifting platform 3 and is connected to the endotracheal cannula through a Luer lock joint, and the endotracheal cannula is used to perform endotracheal intubation on experimental animals.

[0043] A pressure-stabilizing tank 12 and a pneumatic valve 11 are also placed on the top plane of the gas storage area. A Tedlar sampling bag filled with hyperpolarized gas is arranged in the pressure-stabilizing tank 12. A plurality of pipeline perforations are arranged on the animal bed 1. The air inlet end of the xenon gas pipeline passes through the pipeline perforations and is connected to the air outlet end of the first pneumatic valve. The air inlet end of the first pneumatic valve is connected to the pressure-stabilizing tank 12 through a Luer lock joint; the air outlet end of the exhalation gas pipeline is connected to the air inlet end of the second pneumatic valve, and the air outlet end of the second pneumatic valve is connected to the outside world; the air inlet end of the oxygen gas pipeline passes through the pipeline perforations and extends to the animal bed. 1 is connected to the oxygen storage cylinder; the air inlet end of the sensor air pipeline is also connected to the oxygen air pipeline, the xenon air pipeline, and the exhalation air pipeline; the control ends of the first pneumatic valve and the second pneumatic valve are respectively connected to the air outlet end of a nitrogen air pipeline, and the air inlet end of the oxygen air pipeline, the air inlet ends of the two nitrogen air pipelines, and the other end of the sensor air pipeline are stored and sorted through a cable tie 13, and the cable tie 13 is arranged behind the pressure stabilizing tank 12; the pressure stabilizing tank 12, the first pneumatic valve, the second pneumatic valve, and the cable tie 13 are all fixedly arranged at the rear of the animal bed 1;

[0044] The oxygen gas pipeline is used to introduce oxygen, and after the oxygen is introduced, it is inhaled by the experimental animals through the cannula connecting tube; the xenon gas pipelines are used to introduce xenon gas, and after the first pneumatic valve is opened, the xenon gas in the pressure regulating tank 12 is inhaled by the experimental animals through the xenon gas pipeline and the cannula connecting tube; the gas exhaled by the experimental animals goes to the outside through the cannula connecting tube and the exhalation gas pipeline; the sensor gas pipeline is used to measure the mouth-end pressure of the experimental animals under free breathing, thereby reflecting the lung pressure of the animals under free breathing; the two nitrogen gas pipelines are used to control the opening and closing of the first pneumatic valve and the second pneumatic valve respectively.

[0045] The bottom of the end of the animal bed 1 is also provided with a tail motor fixing slot 22 adapted to the top of the motor of the magnetic resonance scanner. The motor of the magnetic resonance scanner is placed in the motor slot of the magnetic resonance scanner, and the tail motor fixing slot 22 at the bottom of the animal bed 1 is placed on the top of the motor of the magnetic resonance scanner.

[0046] The device also includes a simulation chamber, which includes a low-temperature coil simulation component 17, a low-temperature coil shell simulation component 18, an L-shaped bottom support platform 19, and a central tube simulation component 20. The low-temperature coil simulation component 17 and the low-temperature coil shell simulation component 18 are both transparent. The low-temperature coil simulation component 17 and the low-temperature coil shell simulation component 18 are fixed on the vertical plate of the bottom support platform 19; the central tube simulation component 20 is fixed on the horizontal plate of the bottom support platform 19, the gas storage area is placed on the flat working plane of the central tube simulation component 20, the front part of the animal placement area extends into the low-temperature coil shell simulation component 18, and the experimental animal is placed below the low-temperature coil simulation component 17;

[0047] A placement plane 23 is provided at the bottom of the gas storage area, which is convenient for placing the animal bed 1 on the flat working plane of the central tube simulation component 20. The radius of the end of the gas storage area is larger than the radius of the end of the animal placement area. Since the actual low-temperature coil shell is at a certain height from the working plane, and the inner diameter of the low-temperature coil shell is smaller than the central tube, the low-temperature coil simulation component 17 located inside the low-temperature coil shell is higher than the working plane by a certain distance, so that the front animal placement area is raised to a set height from the working plane, so that the experimental animals in the animal placement trough can be close to the low-temperature coil simulation component 17, and the experimental animals in the animal placement trough can be close to the low-temperature coil simulation component 17 by adjusting the fine-tuning long bolts 4 and the lifting platform 3.

[0048] Since the cryocoil is fixed in the cavity of the magnetic resonance scanner during use, and the close fit between the cryocoil and the animal's brain during use greatly affects the imaging quality, it is impossible to send the animal placement area of ​​the animal bed 1 under the cryocoil and ensure close fit by conventional laser positioning. The simulation chamber restores the relative positions of the cryocoil and the animal bed 1 in the cavity of the magnetic resonance scanner 1:1, ensuring that the brain of the experimental animal is close to the cryocoil after the animal bed 1 enters the cavity of the magnetic resonance scanner. The cryocoil simulation component 17 and the cryocoil shell simulation component 18 are made of transparent materials, which are convenient for observing and adjusting the fit between the experimental animal and the cryocoil simulation component 17. Therefore, after setting the positions of the front and rear positioning rods 2 and the fine-tuning long bolts 4 of the animal bed 1 through the simulation chamber, the animal bed 1 is sent into the magnetic resonance scanner to ensure the fit between the experimental animal and the real cryocoil.

[0049] Before using the present invention to conduct a magnetic resonance experiment, the experimental animal is placed on the animal bed 1, and the head of the experimental animal is located on the lifting platform 3, and the tracheal cannula is connected through the Luer lock joint, and then the animal bed 1 is placed in the simulation chamber, and the positions of the front and rear positioning rods 2 are adjusted so that the brain of the experimental animal is located below the low-temperature coil simulation component 17, and the fine-tuning long bolts 4 are adjusted to adjust the animal placement area to a suitable height. The lifting angle of the lifting platform 3 and the fine-tuning long bolts 4 are staggered to ensure that the brain of the experimental animal is close to the low-temperature coil simulation component 17, ensuring a higher filling factor of the coil, and then the animal bed 1 is taken out of the simulation chamber, and fixed on the motor of the magnetic resonance scanner through the tail motor fixing groove 22 that matches the motor slot on the magnetic resonance scanner, and the cannula connecting tube is connected to the tracheal cannula of the ventilator through the Luer lock joint. The hyperpolarized gas used for imaging is collected in a Tedlar sampling bag, sealed in a pressure stabilizing tank 12, and the pressure stabilizing tank 12 is placed in a corresponding position on the animal bed 1, and then the animal bed 1 is pushed to the corresponding position in the cavity of the magnetic resonance scanner, and then the experiment is started.

[0050] The animal bed 1 and the simulation chamber can be made by 3D printing using PLA or PEEK materials, and the transparent cover can be made by processing organic glass. They have high structural strength and integration, and the construction steps and workflow are simple.

[0051] It should be noted that the embodiments described in the present invention are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the described embodiments or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the attached claims.

Claims

1. A cryo coil-based method for the brain 129 An animal bed device for Xe MRI comprises an animal bed (1), characterized in that: The front part of the animal bed (1) is an animal placement area. The top surface of the animal bed (1) is a plane. The end cross-section of the animal placement area is semicircular. The top plane of the animal placement area is provided with an animal placement slot along the length direction of the animal bed (1). The two sides of the animal placement slot are side blocks (5). The side blocks (5) are provided with elongated limiting holes along the length direction of the animal bed (1). The limiting holes penetrate the front part of the side blocks (5). Front and rear positioning rods (2) are slidably arranged in the limiting holes. The animal placement slot is also provided with an installation slot. A lifting platform (3) is arranged in the installation slot. A center rod (24) along the width direction of the animal bed (1) is also arranged in the installation slot. The two ends of the center rod (24) are connected to the ends of the installation slot. The trough wall is fixedly connected, and the fixed part at the rear end of the lifting platform (3) is hinged to the center rod (24); the lifting platform (3) is provided with an insertion opening (21), and the bottom of the lifting platform (3) is provided with a support rod installation groove (25), the support rod installation groove (25) is perpendicular to the center rod (24), a support rod (7) is arranged in the support rod installation groove (25), the fixed end of the support rod (7) is hinged to the support rod installation groove (25), and the other end of the support rod (7) is a rotating end; a first threaded hole penetrating the animal bed (1) is provided at the center of the bottom of the animal placement trough, and a fine-tuning long bolt (4) with thread matching is inserted into the first threaded hole, and the bottom of the fine-tuning long bolt (4) protrudes out of the bottom of the animal placement area; The bottom of the animal placement area is a transparent bottom cover (6), the top of the transparent bottom cover (6) is a plane, the top of the transparent bottom cover (6) is a hose placement plane, a plurality of hose clamping slots (8) are arranged side by side on the hose placement plane along the length direction of the animal bed (1), a five-way connector (14) is arranged in front of the hose clamping slot (8), the five-way connector (14) is placed in a connector placement slot provided on the animal bed (1), the transparent bottom cover (6) is fixedly connected to the hose placement plane, and the bottom of the fine-tuning long bolt (4) extends out of the transparent bottom cover (6); an animal fixing cover (9) is also arranged above the animal placement slot, and the animal fixing cover (9) is fixedly connected to the top of the side block (5); The rear part of the animal bed (1) is a gas storage area. The end section of the gas storage area is semicircular. The gas storage area is provided with an oxygen gas pipeline, a xenon gas pipeline, an exhalation gas pipeline, and a sensor gas pipeline. The cross section of the hose slot (8) is arched. Each gas pipeline is clamped in a hose slot (8). The five-way connector (14) includes a cannula interface and four gas pipeline interfaces. The four gas pipeline interfaces of the five-way connector (14) are respectively connected to the outlet end of the oxygen gas pipeline, the outlet end of the xenon gas pipeline, the inlet end of the exhalation gas pipeline, and the inlet end of the sensor gas pipeline. The cannula interface is located at the top of the five-way connector (14). One end of the cannula connecting tube is connected to the cannula interface of the five-way connector (14). The other end of the cannula connecting tube passes through the cannula opening (21) of the lifting platform (3) and is connected to the tracheal cannula through a Luer lock joint. A pressure-suppressing tank (12) is also placed on the top plane of the gas storage area. A Tedlar sampling bag containing hyperpolarized gas is arranged in the pressure-suppressing tank (12). A plurality of pipeline perforations are arranged on the animal bed (1). The air inlet end of the xenon gas pipeline passes through the pipeline perforations and is connected to the air outlet end of the first pneumatic valve. The air inlet end of the first pneumatic valve is connected to the pressure-suppressing tank (12) via a Luer lock joint. The air outlet end of the exhalation gas pipeline is connected to the air inlet end of the second pneumatic valve. The air outlet end of the second pneumatic valve is connected to the outside. The air inlet end of the oxygen gas pipeline passes through the pipeline perforations and extends to the outside of the animal bed (1) to connect to the oxygen storage gas. The first pneumatic valve and the second pneumatic valve are connected to the oxygen gas pipeline, the xenon gas pipeline, and the exhalation gas pipeline; the control ends of the first pneumatic valve and the second pneumatic valve are respectively connected to the gas outlet end of a nitrogen gas pipeline; the oxygen gas pipeline, the two nitrogen gas pipelines, and the other end of the sensor gas pipeline are stored and arranged by a cable tie (13); the cable tie (13) is arranged behind the pressure stabilizing tank (12); the pressure stabilizing tank (12), the first pneumatic valve, the second pneumatic valve, and the cable tie (13) are all fixedly arranged at the rear of the animal bed (1).

2. A method for treating a brain based on a cryo coil according to claim 1. 129 The animal bed device of Xe MRI is characterized by: The installation groove is also provided with a plurality of limiting grooves (10) arranged side by side, and the rotating end of the support rod (7) is placed in the limiting groove (10) to fix the lifting angle of the lifting platform (3); the rotating end of the support rod (7) is placed in different limiting grooves (10) to adjust the lifting platform (3) to different lifting angles.

3. A method for treating a brain based on a cryo coil according to claim 2. 129 The animal bed device of Xe MRI is characterized by: The top of the side block (5) is provided with a plurality of positioning holes (15) evenly distributed along the length direction of the animal bed (1) and communicating with the limiting holes. The front and rear positioning rods (2) are also provided with positioning holes (15). The latch is inserted into the positioning holes (15) of the front and rear positioning rods (2) and one of the positioning holes (15) of the side block (5).

4. A method for treating a brain based on a cryo coil according to claim 3. 129 The animal bed device of Xe MRI is characterized by: The radius of the end of the gas storage area is greater than the radius of the end of the animal placement area, and a placement plane (23) is provided at the bottom of the gas storage area.

5. A method for treating a brain based on a cryo coil according to claim 4. 129 The animal bed device of Xe MRI is characterized by: A tail motor fixing groove (22) is also provided at the bottom of the end of the animal bed (1).

6. A method for treating a brain based on a cryo coil according to claim 5. 129 The animal bed device of Xe MRI is characterized by: The device also includes a simulation chamber, which includes a low-temperature coil simulation component (17), a low-temperature coil shell simulation component (18), an L-shaped bottom support platform (19), and a central tube simulation component (20). The low-temperature coil simulation component (17) and the low-temperature coil shell simulation component (18) are both transparent. The low-temperature coil simulation component (17) and the low-temperature coil shell simulation component (18) are fixed on the vertical plate of the bottom support platform (19); the central tube simulation component (20) is fixed on the horizontal plate of the bottom support platform (19); the animal bed (1) is placed in the central tube simulation component (20), and the front of the animal placement area extends into the low-temperature coil shell simulation component (18).

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