A non-invasive sampling device and method for giant pandas

Abstract

The present invention provides a non-invasive sampling device and method for giant pandas, and relates to the technical field of animal research. The sampling device comprises a shell, the top of which is fixedly connected to a protective cage; a plurality of combing and brushing mechanisms are arranged on the protective cage; a plurality of hair collecting mechanisms are arranged in the shell in a communication manner; a saliva collecting mechanism is arranged on the shell, and a salt brick is connected to the saliva collecting mechanism. The present invention places food for inducing giant pandas to eat in the protective cage, and the combing and brushing mechanism is used to comb the giant pandas when the giant pandas eat, so that the hair of the giant pandas that naturally detaches from the giant pandas separates and falls off, and the hair collecting mechanism collects and stores the hair of the giant pandas that separates and falls off, so as to obtain the hair samples of the giant pandas; and the salt bricks are used to induce the giant pandas to lick the saliva collecting mechanism, and the saliva of the giant pandas is collected and stored by the saliva collecting mechanism, so as to obtain the saliva samples of the giant pandas, thereby realizing non-invasive sampling of the giant pandas.

Description

Technical Field

[0001] The invention relates to the technical field of animal research, and in particular to a non-invasive sampling device and method for giant pandas. Background Art

[0002] The giant panda is the only mammal belonging to the order Carnivora, family Ursidae, subfamily Pandainae, and genus Ailuropoda. It has only two subspecies, namely the Sichuan subspecies of giant panda and the Qinling subspecies of giant panda. They have lived on Earth for at least 8 million years and are known as "living fossils" and "Chinese national treasures". They are China's first-class protected animals, the image ambassador of the World Wildlife Fund, and the flagship species for the protection of biodiversity in the world.

[0003] Obtaining the genetic information DNA of giant pandas not only plays an important role in individual identification, but also has important value in analyzing the pedigree of giant pandas and even health diagnosis. At present, the genetic information DNA of animals is mainly obtained by sampling devices to obtain the saliva or hair of animals, and the genetic information DNA is extracted from the saliva or hair of animals. However, because giant pandas are too rare, improper operation of using conventional sampling devices to obtain the saliva or hair of giant pandas may cause harm to giant pandas. Although giant pandas look cute, they are "beasts" with a bite force of 1300N-2000N. When conventional sampling devices are improperly operated to cause harm to giant pandas, they will stimulate the aggressiveness of giant pandas and pose safety risks, thus requiring non-traumatic sampling of giant pandas.

[0004] Therefore, how to achieve non-invasive sampling of giant pandas is a problem that needs to be solved urgently in this technical field. Summary of the invention

[0005] The purpose of the present invention is to provide a non-invasive sampling device and method for giant pandas to improve the above problems. In order to achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] The present application provides a non-invasive sampling device for giant pandas, comprising:

[0007] A shell, the top of which is fixedly connected to a protective cage, wherein food for inducing the giant panda to eat is placed in the protective cage;

[0008] A plurality of combing and brushing mechanisms, wherein the combing and brushing mechanisms are arranged on the protective cage and are used to comb and brush the hair of the giant panda so that the naturally detached hair of the giant panda is separated and falls off;

[0009] A plurality of hair collecting mechanisms, the hair collecting mechanisms are connected and arranged in the shell, the hair collecting mechanisms are arranged corresponding to the protective cage, and the hair collecting mechanisms are used to collect the hair separated and dropped by the giant panda;

[0010] A saliva collecting mechanism is arranged on the shell, and is connected to a salt brick. The salt brick is used for the giant panda to lick, so that when the giant panda licks the salt brick, the saliva collecting mechanism collects the giant panda's saliva.

[0011] Preferably, it also includes a mesh bottom plate, the shell is fixedly connected to the middle position of the mesh bottom plate, the mesh bottom plate is used for giant pandas to step on, a plurality of internal threaded tubes are connected and arranged on the mesh bottom plate, the internal threaded tubes are threadedly connected with anchor bolts, an anchor rod is integrally arranged at the end of the anchor bolt, and a plurality of inverted triangle blocks are integrally arranged on the peripheral wall of the anchor rod.

[0012] Preferably, the protective cage comprises:

[0013] A lower rectangular frame, wherein the lower rectangular frame is fixedly connected to the housing, a plurality of fixing columns are fixedly connected to the top of the lower rectangular frame, a plurality of T-shaped sliding holes are opened at the top of the lower rectangular frame, and the T-shaped sliding holes are located between two adjacent fixing columns;

[0014] A plurality of clamping blocks I, wherein the plurality of clamping blocks I are symmetrically fixedly connected in the T-shaped sliding hole, and two ends of the clamping blocks I are respectively connected to one end of the spring I;

[0015] An upper rectangular frame, wherein the upper rectangular frame is fixedly connected to the plurality of fixed columns, a cover plate is connected to the top end of the upper rectangular frame, and a plurality of T-shaped slide grooves are provided at the bottom end of the upper rectangular frame, wherein the T-shaped slide grooves are arranged corresponding to the T-shaped slide holes;

[0016] A plurality of clamping blocks II, wherein the plurality of clamping blocks II are symmetrically fixedly connected in the T-shaped slide groove, and the two ends of the clamping blocks II are respectively connected to one end of the spring II;

[0017] A plurality of protective rods, wherein the bottom end of the protective rod is fixedly connected with a T-shaped slider I, the T-shaped slider I is slidably connected in the T-shaped slide hole, the two sides of the T-shaped slider I are respectively connected to the other end of the corresponding spring I, the top end of the protective rod is fixedly connected with a T-shaped slider II, the T-shaped slider II is slidably connected in the T-shaped slide groove, the two sides of the T-shaped slider II are respectively connected to the other end of the corresponding spring II, and the combing mechanism is arranged on the protective rod.

[0018] Preferably, the combing mechanism comprises:

[0019] An antistatic shell is fixedly connected to the protective rod, a plurality of antistatic comb teeth are arranged in an array on the antistatic shell, and anti-scratch balls are integrally arranged at the ends of the antistatic comb teeth.

[0020] Preferably, the hair collecting mechanism comprises:

[0021] A storage box, wherein a connection port is provided on the peripheral wall of the shell, a U-shaped guide plate is fixedly connected in the shell, the storage box is inserted into the connection port, the bottom of the storage box is slidably connected to the U-shaped guide plate, a storage port is provided at the top of the shell, the storage box is located below the storage port, and a panel of the storage box is connected to the shell by bolts;

[0022] An inclined top seat, wherein a plurality of T-shaped slots are arranged at the rear end of the inclined top seat, and a plurality of T-shaped clip strips are connected to the peripheral wall of the shell, and the T-shaped clip strips are engaged with the T-shaped slots;

[0023] A winding assembly, the winding assembly is arranged in the storage box, and the winding assembly is used to store the giant panda hair that falls into the storage box;

[0024] A detection drive component is arranged in the protection cage, the detection drive component is transmission-connected with the winding component, and the detection drive component is used to drive the winding component to wind when the giant panda is eating.

[0025] Preferably, the winding assembly comprises:

[0026] An active roller, the active roller is rotatably connected to one side of the storage box, the active roller is connected to a transmission gear, the transmission gear is located outside the storage box, and the transmission gear is transmission-connected to the detection drive assembly;

[0027] A driven roller, the driven roller being rotatably connected to the other side of the storage box;

[0028] A collecting film belt is wound around the driven roller, the head end of the collecting film belt is bonded to the active roller, the collecting film belt comprises a PE film, a silicone film is connected to the PE film, and the silicone film is used to adhere to the giant panda's hair through viscosity.

[0029] Preferably, the detection drive component comprises:

[0030] A light curtain sensor, wherein the transmitting end of the light curtain sensor is fixedly connected to the upper part of the panel of the protection cage, and the receiving end of the light curtain sensor is fixedly connected to the lower part of the panel of the protection cage, and the light curtain sensor is used to detect whether the giant panda extends its palm into the protection cage to eat;

[0031] A servo motor is fixedly connected in the housing. The servo motor is transmission-connected to a driving gear, and the driving gear is meshed with the transmission gear.

[0032] Preferably, the saliva collection mechanism comprises:

[0033] A box body, the box body is arranged in communication with the shell body;

[0034] A limit seat, the limit seat is fixedly connected to the top of the shell, the limit seat is located outside the protective cage, the top of the limit seat is provided with an annular guide groove, the peripheral wall of the limit seat is provided with a V-shaped guide groove, the top of the V-shaped guide groove is connected with the annular guide groove, the bottom of the V-shaped guide groove is connected with a guide pipe, and the guide pipe is connected and arranged in the box body;

[0035] A limiting column, wherein the limiting column is connected to the middle position of the top end of the limiting seat, a plurality of limiting openings are provided on the limiting column, a limiting claw is hinged in the limiting opening, a plurality of bayonet openings are provided at the bottom of the salt brick, and the limiting claw is engaged in the bayonet openings;

[0036] A limiting ring, wherein the limiting ring is threadedly connected to the peripheral wall of the limiting column, and the inner wall of the limiting ring abuts against the limiting claw;

[0037] A servo indexing plate is arranged in the box, and an annular bracket is connected to the servo indexing plate. A plurality of pressure sensors are arranged on the bottom plate of the annular bracket. A plurality of test tubes are mounted on the annular bracket. The bottom ends of the test tubes are against the pressure sensors, and the top ends of the test tubes are arranged corresponding to the ends of the guide pipes. Preservation liquid is arranged in the test tubes.

[0038] Preferably, the limit seat and the limit column are also connected to each other with a pipeline channel I, a pipeline channel II is opened in the middle position of the salt brick, a liquid storage tank and a micro pump are arranged in the box body, deionized water is stored in the liquid storage tank, the input end of the micro pump is connected to the liquid storage tank, and the output end of the micro pump is connected to a hose, the hose is gap-fittedly arranged in the pipeline channel I and the pipeline channel II, and the middle part of the hose is fixedly connected to the inner wall of the box body.

[0039] The present application also discloses a sampling method using the giant panda non-invasive sampling device, comprising the following steps:

[0040] S1. Fix the shell in places where giant pandas often appear;

[0041] S2. Clean and wipe multiple brushing mechanisms, clean and wipe the protective cage and the housing, and then clean and debug the hair collection mechanism;

[0042] S3. Polishing to remove the edges and corners of the salt brick surface, and connecting the polished salt brick to the saliva collection mechanism, and then cleaning and debugging the saliva collection mechanism;

[0043] S4. Place food that will induce the giant panda to eat in the middle of the protective cage, and then the staff will leave;

[0044] S5. After 12 to 72 hours, the staff will check the hair collection situation of the hair collection agency and the saliva collection situation of the saliva collection agency;

[0045] S6. When the hair collection agency collects the hair sample of the giant panda and / or the saliva collection agency collects the saliva sample of the giant panda, the staff will take out the hair sample and / or the saliva sample and store them, and then remove the non-invasive sampling device of the giant panda;

[0046] S7. When the hair collection mechanism fails to collect a hair sample and the saliva collection mechanism fails to collect a saliva sample, replace the food in the protective cage to induce the giant panda to eat, clean and wipe the salt bricks on the saliva collection mechanism, and repeat step S5 after the staff leaves until the giant panda's hair sample and / or saliva sample is collected.

[0047] The beneficial effects of the present invention are:

[0048] The present invention places food for inducing giant pandas to eat in a protective cage, and the giant panda uses a combing mechanism to comb the giant panda while eating, so that naturally detached hair of the giant panda is separated and falls off, and a hair collecting mechanism collects and stores the separated and fallen hair of the giant panda to obtain a hair sample of the giant panda; and the giant panda is induced to lick the saliva collecting mechanism through salt bricks, and the saliva of the giant panda is collected and stored through the saliva collecting mechanism to obtain a saliva sample of the giant panda, thereby realizing non-invasive sampling of the giant panda.

[0049] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or be understood by implementing the embodiments of the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures particularly pointed out in the written description, claims, and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0051] Figure 1 This is a schematic diagram of the structure of this application;

[0052] Figure 2 This is a schematic diagram of the mesh bottom plate structure of the present application;

[0053] Figure 3 This is a schematic diagram of the anchor bolt structure of this application;

[0054] Figure 4 This is a schematic diagram of the protective cage structure of this application;

[0055] Figure 5 The lower rectangular frame connection diagram of this application;

[0056] Figure 6 It is a schematic diagram of the connection of the upper rectangular frame of this application;

[0057] Figure 7 This is a schematic diagram of the brushing mechanism structure of the present application;

[0058] Figure 8 This is a schematic diagram of the shell structure of this application;

[0059] Fig. 9 This is a connection diagram of the hair collection mechanism of the present application;

[0060] Fig.10 This is a schematic diagram of the inclined top seat structure of this application;

[0061] Fig.11 This is a schematic diagram of the internal structure of the shell of this application;

[0062] Fig.12 This is a schematic diagram of the winding assembly structure of the present application;

[0063] Fig.13 This is a schematic diagram of the servo motor connection of this application;

[0064] Fig.14 This is a schematic diagram of the connection of the limit seat of this application;

[0065] Fig.15 This is a schematic diagram of the salt brick structure of this application;

[0066] Fig.16 This is a schematic diagram of the internal structure of the box of this application;

[0067] Markings in the figure: housing 1, U-shaped guide plate 11, storage port 12, T-shaped card strip 13, protective cage 2, lower rectangular frame 21, fixing column 22, T-shaped slide hole 23, card block I 24, spring I 25, upper rectangular frame 26, cover plate 27, T-shaped slide groove 28, card block II 29, spring II 210, protective rod 211, T-shaped slide block I 212, T-shaped slide block II 213, combing and brushing mechanism 3, anti-static housing 31, anti-static comb teeth 32, anti-scratch ball 33, hair collecting mechanism 4, storage box 41, bolt 411, inclined top seat 42, T-shaped card slot 43, winding assembly 44, active roller 441, transmission gear 442, driven roller 443, collection Film collecting belt 444, detection drive assembly 45, light curtain sensor 451, servo motor 452, driving gear 453, saliva collecting mechanism 5, box body 51, limit seat 52, annular guide groove 53, V-shaped guide groove 54, guide pipe 55, limit column 56, limit claw 57, salt brick 58, bayonet 581, pipeline channel II 582, limit ring 59, servo dividing plate 510, annular bracket 511, pressure sensor 512, test tube 513, pipeline channel I 514, liquid storage tank 515, micro pump 516, hose 517, mesh bottom plate 6, internal threaded cylinder 61, anchor bolt 62, anchor rod 63, inverted triangle block 64. DETAILED DESCRIPTION

[0068] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. The components of the embodiments of the present invention generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0069] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. At the same time, in the description of the present invention, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0070] Embodiment 1:

[0071] like Figure 1 and Fig.11 As shown, this embodiment provides a non-invasive sampling device for giant pandas, comprising:

[0072] A housing 1, the top of which is fixedly connected to a protective cage 2, wherein the protective cage 2 is used to place food for inducing the giant panda to eat;

[0073] A plurality of combing and brushing mechanisms 3, wherein the combing and brushing mechanisms 3 are arranged on the protective cage 2, and the combing and brushing mechanisms 3 are used to comb and brush the hair of the giant panda so that the naturally detached hair of the giant panda is separated and falls off;

[0074] A plurality of hair collecting mechanisms 4, wherein the hair collecting mechanisms 4 are arranged in communication with the housing 1, the hair collecting mechanisms 4 are arranged corresponding to the protective cage 2, and the hair collecting mechanisms 4 are used to collect the hair separated and dropped by the giant panda;

[0075] The saliva collecting mechanism 5 is arranged on the shell 1, and is connected to a salt brick 58, and the salt brick 58 is used for the giant panda to lick, so that when the giant panda licks the salt brick 58, the saliva collecting mechanism 5 collects the saliva of the giant panda.

[0076] It is understandable that when obtaining the saliva and / or hair of the giant panda, the shell 1 is fixedly set in the place where the giant panda appears, and then the multiple combing and brushing mechanisms 3 are cleaned and wiped, and the protective cage 2 and the shell 1 are cleaned and wiped to prevent dust or residue from contaminating the hair sample, and then the hair collection mechanism 4 is cleaned and debugged to ensure the hair collection effect of the hair collection mechanism 4. After grinding and removing the edges and corners on the surface of the salt brick 58, the polished salt brick 58 is connected to the saliva collection mechanism 5, and then the saliva collection mechanism 5 is cleaned and debugged, and then the food that induces the giant panda to eat is placed in the middle position of the protective cage 2, and the staff evacuates. When the giant panda puts its palm into the protective cage 2 to eat food, the hair of the giant panda contacts the combing and brushing mechanism 3, and the combing and brushing mechanism 3 combs the hair of the giant panda in the reverse direction, so that the naturally detached hair of the giant panda separates and falls off, and the separated and fallen hair of the giant panda enters the hair collecting mechanism 4, and the hair of the giant panda is collected and preserved by the hair collecting mechanism 4 to obtain the hair sample of the giant panda. When the giant panda takes food out of the protective cage 2, the combing and brushing mechanism 3 combs the hair of the giant panda in the forward direction to comb and brush the hair of the giant panda flat; the cautious giant panda will be reluctant to eat the food in the protective cage 2, so that the hair sample of the giant panda cannot be obtained, but The panda's regular food lacks salt, so there is a need to supplement salt. Therefore, a salt brick 58 is connected to the saliva collecting mechanism 5, and the salt brick 58 is used to induce the panda to lick the saliva collecting mechanism 5. The salt brick 58 has the effect of promoting saliva secretion, and the salt will not affect the genetic information DNA in the saliva. When the panda licks the salt brick 58, the panda's saliva drips onto the saliva collecting mechanism 5, and the panda's saliva is collected and stored by the saliva collecting mechanism 5 to obtain a saliva sample of the panda. After the staff takes out the panda's hair sample and / or saliva sample, the panda's non-invasive sampling device is removed. The panda's hair and / or saliva samples are separated and the panda's genetic information DNA is extracted from the panda's hair and / or saliva samples. In this technical solution, food that induces the panda to eat is placed in the protective cage 2. When the panda eats, the brushing mechanism 3 brushes the panda's hair so that the panda's hair that is naturally detached from the panda falls off. The hair collecting mechanism 4 collects and stores the separated and fallen hair of the panda to obtain the panda's hair sample. The panda is induced to lick the saliva collecting mechanism 5 through the salt bricks 58. The saliva of the panda is collected and stored through the saliva collecting mechanism 5 to obtain the panda's saliva sample, thereby realizing non-invasive sampling of the panda.

[0077] like Figure 2-Figure 3As shown, it also includes a mesh bottom plate 6, and the shell 1 is fixedly connected to the middle position of the mesh bottom plate 6. The mesh bottom plate 6 is used for the giant panda to step on. A plurality of internal threaded tubes 61 are connected to the mesh bottom plate 6, and the internal threaded tubes 61 are threadedly connected with anchor bolts 62. An anchor rod 63 is integrally provided at the end of the anchor bolt 62, and a plurality of inverted triangle blocks 64 are integrally provided on the peripheral wall of the anchor rod 63.

[0078] It is understandable that the shell 1 is fixedly set in the place where the giant pandas appear, and there is a risk of being pushed by the giant pandas. Therefore, the shell 1 is fixedly connected to the middle position on the mesh bottom plate 6. When the giant panda approaches the shell 1, it must be located on the mesh bottom plate 6. The connection between the shell 1 and the mesh bottom plate 6 prevents the shell 1 from being pushed by the giant panda. At the same time, the mesh structure of the mesh bottom plate 6 can be better integrated with the environment and reduce the giant panda’s vigilance; after the mesh bottom plate 6 is set in the place where the giant pandas appear, the anchor rod 63 is inserted into the internal threaded tube 61, and then the anchor rod 63 is embedded in the ground, and then the anchor bolt 62 is turned clockwise to thread the anchor bolt 62 with the internal threaded tube 61. When the anchor bolt 62 is turned clockwise, the anchor rod 63 rotates synchronously in the ground, so that the multiple inverted triangle blocks 64 are rotated and embedded in the soil for anchoring, thereby strengthening the connection strength between the mesh bottom plate 6 and the ground.

[0079] like Figure 4-Figure 6 As shown, the protective cage 2 comprises:

[0080] A lower rectangular frame 21, wherein the lower rectangular frame 21 is fixedly connected to the housing 1, a plurality of fixing columns 22 are fixedly connected to the top of the lower rectangular frame 21, a plurality of T-shaped sliding holes 23 are opened at the top of the lower rectangular frame 21, and the T-shaped sliding holes 23 are located between two adjacent fixing columns 22;

[0081] A plurality of clamping blocks Ⅰ24, wherein the plurality of clamping blocks Ⅰ24 are symmetrically fixedly connected in the T-shaped sliding hole 23, and the two ends of the clamping blocks Ⅰ24 are respectively connected to one end of a spring Ⅰ25;

[0082] An upper rectangular frame 26, the upper rectangular frame 26 is fixedly connected to the plurality of fixed columns 22, a cover plate 27 is connected to the top of the upper rectangular frame 26, and a plurality of T-shaped slide grooves 28 are provided at the bottom of the upper rectangular frame 26, and the T-shaped slide grooves 28 are arranged corresponding to the T-shaped slide holes 23;

[0083] A plurality of clamping blocks II 29, wherein the plurality of clamping blocks II 29 are symmetrically fixedly connected in the T-shaped slide groove 28, and the two ends of the clamping blocks II 29 are respectively connected to one end of the spring II 210;

[0084] A plurality of protective rods 211, the bottom end of each protective rod 211 is fixedly connected with a T-shaped slider I 212, which is slidably connected in the T-shaped slide hole 23, and the two sides of each T-shaped slider I 212 are respectively connected to the other end of the corresponding spring I 25, the top end of each protective rod 211 is fixedly connected with a T-shaped slider II 213, which is slidably connected in the T-shaped slide groove 28, and the two sides of each T-shaped slider II 213 are respectively connected to the other end of the corresponding spring II 210, and the brushing mechanism 3 is arranged on the protective rod 211.

[0085] It is understandable that when the gap of the protective cage 2 is small, the panda's palm cannot reach into the protective cage 2 to take food, and when the gap of the protective cage 2 is large, the combing and brushing mechanism 3 cannot effectively comb the panda's hair; therefore, a plurality of protective rods 211 are slidably connected between the lower rectangular frame 21 and the upper rectangular frame 26, and the sliding range of the bottom end of the protective rod 211 is limited by the block I 24, and the sliding range of the top end of the protective rod 211 is limited by the block II 29, and the bottom end of the protective rod 211 is provided with elastic support by the spring I 25, and the top end of the protective rod 211 is provided with elastic support by the spring II 210, and the combing and brushing mechanism 3 is arranged on the protective rod 211. When the giant panda's palm reaches into the protective cage 2 to take food, the combing and brushing mechanism 3 is in direct contact with the giant panda's hair. As the giant panda's palm reaches in, the protective rods 211 on both sides of the giant panda's arms slide along the T-shaped slide holes 23 and the T-shaped slide grooves 28, so that the gap between the two protective rods 211 increases, making it easier for the giant panda to take food. The pulling and pushing forces applied to the protective rods 211 by the springs Ⅰ25 and Ⅱ210 make the combing and brushing mechanism 3 closely abut against the giant panda's arms, thereby ensuring the combing and brushing effect of the combing and brushing mechanism 3. After the giant panda takes the food, the protective rods 211 are driven to reset by the springs Ⅰ25 and Ⅱ210.

[0086] like Figure 7 As shown, the combing mechanism 3 includes:

[0087] The antistatic housing 31 is fixedly connected to the protection rod 211 . A plurality of antistatic comb teeth 32 are arranged in an array on the antistatic housing 31 . Anti-scratch balls 33 are integrally provided at the ends of the antistatic comb teeth 32 .

[0088] It can be understood that the anti-static shell 31 is used to prevent the giant panda's hair from being adsorbed on the protective rod 211, and the giant panda's hair is combed and brushed by multiple anti-static comb teeth 32. At the same time, static electricity can be avoided during the combing and brushing process, thereby ensuring that the giant panda's separated and fallen hair can fall smoothly into the hair collection mechanism 4 for collection and preservation, and the anti-scratch ball 33 integrally arranged at the end of the anti-static comb teeth 32 can prevent the end of the anti-static comb teeth 32 from causing scratches to the giant panda.

[0089] like Figure 8-Figure 11 As shown, the hair collecting mechanism 4 comprises:

[0090] A storage box 41, a connection port is provided on the peripheral wall of the shell 1, a U-shaped guide plate 11 is fixedly connected to the shell 1, the storage box 41 is inserted into the connection port, the bottom of the storage box 41 is slidably connected to the U-shaped guide plate 11, a storage port 12 is provided at the top of the shell 1, the storage box 41 is located below the storage port 12, and the panel of the storage box 41 is connected to the shell 1 by bolts 411;

[0091] An inclined top seat 42, wherein a plurality of T-shaped slots 43 are disposed at the rear end of the inclined top seat 42, and a plurality of T-shaped clip strips 13 are connected to the peripheral wall of the housing 1, and the T-shaped clip strips 13 are engaged with the T-shaped slots 43;

[0092] A winding assembly 44, wherein the winding assembly 44 is disposed in the storage box 41, and the winding assembly 44 is used to store the giant panda hair that falls into the storage box 41;

[0093] A detection drive component 45 is disposed in the protection cage 2. The detection drive component 45 is transmission-connected to the reeling component 44. The detection drive component 45 is used to drive the reeling component 44 to reel when the giant panda is eating.

[0094] It can be understood that when the panda's palm reaches into the protective cage 2 to take food, the combing and brushing mechanism 3 combs the panda's hair in reverse, so that the panda's naturally detached hair separates and falls, and the separated and fallen hair falls into the reeling assembly 44 from the storage port 12. When the detection drive assembly 45 detects that the panda's palm reaches into the protective cage 2, it drives the reeling assembly 44 to reel, and the panda's hair that falls into the storage box 41 is preserved by the reeling assembly 44; when replacing the reeling assembly 44 or collecting hair samples, the connection between the inclined top seat 42 and the shell 1 is disassembled, and then the bolts 411 connected to the panel of the storage box 41 are disassembled, and then the storage box 41 is taken out from the connection port, so that the reeling assembly 44 and the detection drive assembly 45 are connected. 5 is connected and separated; after replacing the winding assembly 44 or collecting the hair sample, the storage box 41 is inserted from the connecting port, and the panel of the storage box 41 is connected to the shell 1 by the bolt 411, the front and rear movable positions of the storage box 41 are limited, and the left and right movable positions of the storage box 41 are limited by the U-shaped guide plate 11. When the storage box 41 is connected in place, the winding assembly 44 is connected to the detection drive assembly 45, and then the inclined top seat 42 is connected to the shell 1 through the cooperation of the T-shaped slot 43 and the T-shaped card strip 13. The storage box 41 is protected by the inclined top seat 42 to prevent the giant panda from damaging the storage box 41 and / or the winding assembly 44, and the inclined top seat 42 is convenient for the giant panda to obtain food in the protective cage 2 after stepping on it, or for the giant panda to lean on it to eat food.

[0095] like Fig.12 As shown, the winding assembly 44 includes:

[0096] An active roller 441, the active roller 441 is rotatably connected to one side of the storage box 41, the active roller 441 is connected to a transmission gear 442, the transmission gear 442 is located outside the storage box 41, and the transmission gear 442 is transmission-connected to the detection drive assembly 45;

[0097] A driven roller 443, the driven roller 443 is rotatably connected to the other side of the storage box 41;

[0098] The collecting film belt 444 is wound around the driven roller 443, the head end of the collecting film belt 444 is bonded to the active roller 441, and the collecting film belt 444 includes a PE film, a silicone film is connected to the PE film, and the silicone film is used to adhere to the giant panda's hair through viscosity.

[0099] It can be understood that after the naturally detached hair of the giant panda separates and falls, it falls from the storage port 12 onto the silicone film of the collecting film belt 444, and the giant panda's hair is adhered to it by the viscosity of the silicone film to prevent the giant panda's hair from falling off. When the detection drive component 45 detects that the giant panda's palm is inserted into the protective cage 2, the driving transmission gear 442 drives the active roller 441 to rotate, so that the collecting film belt 444 adhered with the giant panda's hair is rolled up onto the active roller 441, and after the collecting film belt 444 is rolled up onto the active roller 441, the silicone film and the PE film are in contact with each other, so that the giant panda's hair is sealed and preserved. When taking out the giant panda's hair sample, the storage box 41 is taken out from the connecting port, and then the collecting film belt 444 is removed from the active roller 441 and the driven roller 443, and then the giant panda's hair sample is removed from the silicone film and collected and preserved.

[0100] like Figure 5-Figure 6 and Fig.13 As shown, the detection drive component 45 includes:

[0101] A light curtain sensor 451, wherein the transmitting end of the light curtain sensor 451 is fixedly connected to the upper part of the panel of the protection cage 2, and the receiving end of the light curtain sensor 451 is fixedly connected to the lower part of the panel of the protection cage 2. The light curtain sensor 451 is used to detect whether the giant panda extends its palm into the protection cage 2 to eat;

[0102] A servo motor 452 is fixedly connected in the housing 1 . The servo motor 452 is transmission-connected to a driving gear 453 . The driving gear 453 is meshed with the transmission gear 442 .

[0103] It should be noted that a controller is provided in the shell 1 shown, and the control includes an acquisition module, a storage module and a processing module. The acquisition module is connected to the light curtain sensor 451, the storage module is used to store configuration parameters, and the processing module is connected to the servo motor 452. A battery is also provided in the shell 1, and the battery is used for power supply.

[0104] It can be understood that when the giant panda's palm is inserted into the protective cage 2, part of the light curtain of the light curtain sensor 451 is blocked, and the acquisition module collects the trigger signal of the light curtain sensor 451, and transmits the trigger signal to the processing module. After receiving the trigger signal, the processing module drives the servo motor 452 to rotate a set number of circles, so that the servo motor 452 drives the transmission gear 442 to rotate through the driving gear 453, so that the active roller 441 reels the collection film belt 444, thereby preserving the giant panda's hair.

[0105] like Figure 14-16 and Fig. 9 As shown, the saliva collection mechanism 5 comprises:

[0106] A box body 51, wherein the box body 51 is disposed in communication with the housing 1;

[0107] A limit seat 52, the limit seat 52 is fixedly connected to the top of the shell 1, the limit seat 52 is located outside the protection cage 2, the top of the limit seat 52 is provided with an annular guide groove 53, the peripheral wall of the limit seat 52 is provided with a V-shaped guide groove 54, the top of the V-shaped guide groove 54 is connected to the annular guide groove 53, the bottom of the V-shaped guide groove 54 is connected to a guide pipe 55, and the guide pipe 55 is connected and arranged in the box body 51;

[0108] A limiting column 56, wherein the limiting column 56 is connected to the middle position of the top end of the limiting seat 52, and a plurality of limiting openings are formed on the limiting column 56, and a limiting claw 57 is hinged in the limiting opening. A plurality of bayonet openings 581 are formed at the bottom of the salt brick 58, and the limiting claw 57 is engaged in the bayonet openings 581;

[0109] A limiting ring 59, wherein the limiting ring 59 is threadedly connected to the peripheral wall of the limiting column 56, and the inner wall of the limiting ring 59 abuts against the limiting claw 57;

[0110] A servo indexing plate 510 is disposed in the box body 51, and an annular bracket 511 is connected to the servo indexing plate 510. A plurality of pressure sensors 512 are disposed on the bottom plate of the annular bracket 511. A plurality of test tubes 513 are mounted on the annular bracket 511. The bottom ends of the test tubes 513 abut against the pressure sensors 512, and the top ends of the test tubes 513 are disposed corresponding to the end of the guide pipe 55. The test tubes 513 are provided with a preservation solution.

[0111] It can be understood that after grinding away the edges and corners on the surface of the salt brick 58, the salt brick 58 is placed on the limiting column 56, and the multiple limiting claws 57 are engaged into the bayonet 581 on the salt brick 58, and then the limiting ring 59 is threadedly connected to the peripheral wall of the limiting column 56, and the movable position of the multiple limiting claws 57 is limited by the limiting ring 59, so that the salt brick 58 is fixed on the limiting column 56, and then the multiple test tubes 513 containing the preservation solution are respectively mounted on the annular bracket 511, and the bottom end of the test tube 513 is against the pressure sensor 512; when the giant panda licks the salt brick 58, the saliva dripping from the giant panda flows into the annular guide groove 53, and the annular guide groove 53 guides the saliva to flow into the V-shaped guide groove 54. After the V-shaped guide groove 54 collects the saliva, the guide pipe 55 guides the saliva to flow into the test tube 513, and the panda's saliva is preserved by the preservation solution in the test tube 513; when the pressure sensor 512 detects that the total weight of the test tube 513 reaches the set value, the servo indexing plate 510 drives the annular bracket 511 to rotate the set angle, so that the next test tube 513 that has not collected saliva corresponds to the guide pipe 55, and the test tube 513 that has not collected saliva continues to collect saliva. When the weight values ​​measured by multiple pressure sensors 512 all reach the set value, the servo indexing plate 510 stops rotating to avoid mixing and contamination of the giant panda's saliva samples, thereby realizing the collection of the giant panda's saliva.

[0112] It should be noted that the servo indexing plate 510 is connected to the processing module, the multiple pressure sensors 512 are connected to the acquisition module, and the parameter thresholds measured by the pressure sensors 512 are stored in the storage module.

[0113] like Figure 14-16 As shown, the limit seat 52 and the limit column 56 are also connected to each other and are provided with a pipeline channel I 514. A pipeline channel II 582 is opened in the middle position of the salt brick 58. A liquid storage tank 515 and a micro pump 516 are arranged in the box body 51. Deionized water is stored in the liquid storage tank 515. The input end of the micro pump 516 is connected to the liquid storage tank 515. The output end of the micro pump 516 is connected to a hose 517. The hose 517 is arranged in the pipeline channel I 514 and the pipeline channel II 582 with a clearance fit. The middle part of the hose 517 is fixedly connected to the inner wall of the box body 51.

[0114] It is understandable that after the giant panda licks the salt brick 58, saliva will accumulate and remain in the annular guide groove 53 and the V-shaped guide groove 54, so that the amount of saliva entering the test tube 513 cannot meet the demand; therefore, a liquid storage tank 515 and a micro pump 516 are arranged in the box body 51, the input end of the micro pump 516 is connected to the liquid storage tank 515, and the output end of the micro pump 516 is connected to the hose 517, and the hose 517 is passed through the pipeline channel I 514 and the pipeline channel II 582. The storage module stores the first parameter threshold and the second parameter threshold measured by the pressure sensor 512, and the micro pump 516 is connected to the processing module. When the saliva flows into the test tube 513 and reaches the first parameter threshold measured by the pressure sensor 512, the processing module controls the micro pump 516 to start the set time, and the micro pump 516 transports the deionized water stored in the liquid storage tank 515 to Hose 517, the deionized water in the hose 517 flows out to the surface of the salt brick 58, rinses the salt brick 58 and then enters the annular guide groove 53 for rinsing, the deionized water then enters the V-shaped guide groove 54 from the annular guide groove 53 for rinsing, and then flows into the test tube 513 from the guide pipe 55, the deionized water rinses the saliva remaining in the annular guide groove 53 and the V-shaped guide groove 54, after entering the test tube 513, the weight measured by the pressure sensor 512 reaches the second parameter threshold, the servo indexing plate 510 drives the annular bracket 511 to rotate the set angle, the next test tube 513 that has not collected saliva corresponds to the guide pipe 55, the test tube 513 that has not collected saliva continues to collect saliva, and when the weight values ​​measured by multiple pressure sensors 512 all reach the second parameter threshold, the servo indexing plate 510 stops rotating and the micro pump 516 stops starting.

[0115] Embodiment 2:

[0116] The present application also discloses a sampling method using the giant panda non-invasive sampling device, comprising the following steps:

[0117] S1. Fix the shell 1 in a place where giant pandas often appear;

[0118] S2. Clean and wipe multiple brushing mechanisms 3, clean and wipe the protective cage 2 and the housing 1, and then clean and debug the hair collection mechanism 4;

[0119] S3. The edges and corners of the surface of the salt brick 58 are removed by grinding, and the polished salt brick 58 is connected to the saliva collecting mechanism 5, and then the saliva collecting mechanism 5 is cleaned and debugged;

[0120] S4. Place the food that induces the giant panda to eat in the middle of the protective cage 2, and then the staff evacuates;

[0121] S5. After 12 hours to 72 hours, the staff checks the hair collection of the hair collection mechanism 4 and the saliva collection of the saliva collection mechanism 5;

[0122] S6. When the hair collection mechanism 4 collects the hair sample of the giant panda and / or the saliva collection mechanism 5 collects the saliva sample of the giant panda, the staff will take out the hair sample and / or the saliva sample for storage, and then evacuate the giant panda non-invasive sampling device;

[0123] S7. When the hair collection mechanism 4 does not collect a hair sample and the saliva collection mechanism 5 does not collect a saliva sample, replace the food in the protective cage 2 to induce the giant panda to eat, and clean and wipe the salt bricks 58 on the saliva collection mechanism 5. After the staff evacuates, repeat step S5 until the hair sample and / or saliva sample of the giant panda are collected.

[0124] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0125] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A non-invasive sampling device for giant pandas, characterized in that: include: A shell, the top of which is fixedly connected to a protective cage, wherein food for inducing the giant panda to eat is placed in the protective cage; A plurality of combing and brushing mechanisms, wherein the combing and brushing mechanisms are arranged on the protective cage and are used to comb and brush the hair of the giant panda so that the naturally detached hair of the giant panda is separated and falls off; A plurality of hair collecting mechanisms, the hair collecting mechanisms are connected and arranged in the shell, the hair collecting mechanisms are arranged corresponding to the protective cage, and the hair collecting mechanisms are used to collect the hair separated and dropped by the giant panda; A saliva collecting mechanism, the saliva collecting mechanism is arranged on the housing, the saliva collecting mechanism is connected to a salt brick, the salt brick is used for the giant panda to lick, so that when the giant panda licks the salt brick, the saliva collecting mechanism collects the giant panda's saliva; The protective cage comprises: A lower rectangular frame, wherein the lower rectangular frame is fixedly connected to the housing, a plurality of fixing columns are fixedly connected to the top of the lower rectangular frame, a plurality of T-shaped sliding holes are opened at the top of the lower rectangular frame, and the T-shaped sliding holes are located between two adjacent fixing columns; A plurality of clamping blocks I, wherein the plurality of clamping blocks I are symmetrically fixedly connected in the T-shaped sliding hole, and two ends of the clamping blocks I are respectively connected to one end of the spring I; An upper rectangular frame, wherein the upper rectangular frame is fixedly connected to the plurality of fixed columns, a cover plate is connected to the top end of the upper rectangular frame, and a plurality of T-shaped slide grooves are provided at the bottom end of the upper rectangular frame, wherein the T-shaped slide grooves are arranged corresponding to the T-shaped slide holes; A plurality of clamping blocks II, wherein the plurality of clamping blocks II are symmetrically fixedly connected in the T-shaped slide groove, and the two ends of the clamping blocks II are respectively connected to one end of the spring II; A plurality of protective rods, wherein the bottom end of the protective rod is fixedly connected with a T-shaped slider I, the T-shaped slider I is slidably connected in the T-shaped slide hole, the two sides of the T-shaped slider I are respectively connected to the other end of the corresponding spring I, the top end of the protective rod is fixedly connected with a T-shaped slider II, the T-shaped slider II is slidably connected in the T-shaped slide groove, the two sides of the T-shaped slider II are respectively connected to the other end of the corresponding spring II, and the combing mechanism is arranged on the protective rod; The hair collecting mechanism comprises: A storage box, wherein a connection port is provided on the peripheral wall of the shell, a U-shaped guide plate is fixedly connected in the shell, the storage box is inserted into the connection port, the bottom of the storage box is slidably connected to the U-shaped guide plate, a storage port is provided at the top of the shell, the storage box is located below the storage port, and a panel of the storage box is connected to the shell by bolts; An inclined top seat, wherein a plurality of T-shaped slots are arranged at the rear end of the inclined top seat, and a plurality of T-shaped clip strips are connected to the peripheral wall of the shell, and the T-shaped clip strips are engaged with the T-shaped slots; A winding assembly, the winding assembly is arranged in the storage box, and the winding assembly is used to store the giant panda hair that falls into the storage box; A detection drive component is arranged in the protection cage, the detection drive component is transmission-connected with the winding component, and the detection drive component is used to drive the winding component to wind when the giant panda is eating.

2. The giant panda non-invasive sampling device according to claim 1, characterized in that: It also includes a mesh bottom plate, the shell is fixedly connected to the middle position of the mesh bottom plate, the mesh bottom plate is used for giant pandas to step on, a plurality of internal threaded tubes are connected on the mesh bottom plate, the internal threaded tubes are threadedly connected with anchor bolts, an anchor rod is integrally provided at the end of the anchor bolt, and a plurality of inverted triangle blocks are integrally provided on the peripheral wall of the anchor rod.

3. The giant panda non-invasive sampling device according to claim 1, characterized in that: The combing and brushing mechanism comprises: An antistatic shell is fixedly connected to the protective rod, a plurality of antistatic comb teeth are arranged in an array on the antistatic shell, and anti-scratch balls are integrally arranged at the ends of the antistatic comb teeth.

4. The giant panda non-invasive sampling device according to claim 1, characterized in that: The winding assembly comprises: An active roller, the active roller is rotatably connected to one side of the storage box, the active roller is connected to a transmission gear, the transmission gear is located outside the storage box, and the transmission gear is transmission-connected to the detection drive assembly; A driven roller, the driven roller being rotatably connected to the other side of the storage box; A collecting film belt is wound around the driven roller, the head end of the collecting film belt is bonded to the active roller, the collecting film belt comprises a PE film, a silicone film is connected to the PE film, and the silicone film is used to adhere to the giant panda's hair through viscosity.

5. The non-invasive sampling device for giant pandas according to claim 4, characterized in that: The detection drive component comprises: A light curtain sensor, wherein the transmitting end of the light curtain sensor is fixedly connected to the upper part of the panel of the protection cage, and the receiving end of the light curtain sensor is fixedly connected to the lower part of the panel of the protection cage, and the light curtain sensor is used to detect whether the giant panda extends its palm into the protection cage to eat; A servo motor is fixedly connected in the housing. The servo motor is transmission-connected to a driving gear, and the driving gear is meshed with the transmission gear.

6. The giant panda non-invasive sampling device according to claim 1, characterized in that: The saliva collection mechanism comprises: A box body, the box body is arranged in communication with the shell body; A limit seat, the limit seat is fixedly connected to the top of the shell, the limit seat is located outside the protective cage, the top of the limit seat is provided with an annular guide groove, the peripheral wall of the limit seat is provided with a V-shaped guide groove, the top of the V-shaped guide groove is connected with the annular guide groove, the bottom of the V-shaped guide groove is connected with a guide pipe, and the guide pipe is connected and arranged in the box body; A limiting column, wherein the limiting column is connected to the middle position of the top end of the limiting seat, a plurality of limiting openings are provided on the limiting column, a limiting claw is hinged in the limiting opening, a plurality of bayonet openings are provided at the bottom of the salt brick, and the limiting claw is engaged in the bayonet openings; A limiting ring, wherein the limiting ring is threadedly connected to the peripheral wall of the limiting column, and the inner wall of the limiting ring abuts against the limiting claw; A servo indexing plate is arranged in the box, and an annular bracket is connected to the servo indexing plate. A plurality of pressure sensors are arranged on the bottom plate of the annular bracket. A plurality of test tubes are mounted on the annular bracket. The bottom ends of the test tubes are against the pressure sensors, and the top ends of the test tubes are arranged corresponding to the ends of the guide pipes. Preservation liquid is arranged in the test tubes.

7. The non-invasive sampling device for giant pandas according to claim 6, characterized in that: The limit seat and the limit column are also connected to each other and are provided with a pipeline channel I. The middle position of the salt brick is provided with a pipeline channel II. A liquid storage tank and a micro pump are provided in the box body. Deionized water is stored in the liquid storage tank. The input end of the micro pump is connected to the liquid storage tank. The output end of the micro pump is connected to a hose. The hose is gap-fittedly arranged in the pipeline channel I and the pipeline channel II. The middle part of the hose is fixedly connected to the inner wall of the box body.

8. A sampling method using the non-invasive sampling device for giant pandas as described in any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Fix the shell in places where giant pandas often appear; S2. Clean and wipe multiple brushing mechanisms, clean and wipe the protective cage and the housing, and then clean and debug the hair collection mechanism; S3. Polishing to remove the edges and corners of the salt brick surface, and connecting the polished salt brick to the saliva collection mechanism, and then cleaning and debugging the saliva collection mechanism; S4. Place food that will induce the giant panda to eat in the middle of the protective cage, and then the staff will leave; S5. After 12 to 72 hours, the staff will check the hair collection situation of the hair collection agency and the saliva collection situation of the saliva collection agency; S6. When the hair collection agency collects the hair sample of the giant panda and / or the saliva collection agency collects the saliva sample of the giant panda, the staff will take out the hair sample and / or the saliva sample and store them, and then remove the non-invasive sampling device of the giant panda; S7. When the hair collection mechanism fails to collect a hair sample and the saliva collection mechanism fails to collect a saliva sample, replace the food in the protective cage to induce the giant panda to eat, clean and wipe the salt bricks on the saliva collection mechanism, and repeat step S5 after the staff leaves until the giant panda's hair sample and / or saliva sample is collected.

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