Compositions containing both mussel myoglobin and anionic polysaccharide material, methods of making and products thereof

Abstract

The present application relates to the technical field of raw materials, and particularly relates to a composition containing mussel mucin and anionic polysaccharide substance and a preparation method and product thereof. The composition contains, in terms of mass parts, 0.01-0.2 parts of mussel mucin, 0.05-1.0 parts of anionic polysaccharide substance, 0.3-3.0 parts of inorganic salt or citric acid, and 75.0-95.0 parts of water. The present application can form a uniform and transparent composition by adding inorganic salt or citric acid to an aqueous solution containing mussel mucin and anionic polysaccharide substance. This strategy can make mussel mucin and anionic polysaccharide substance compatible and compounded, and has long-term stability, can withstand product shelf life aging test, and greatly improves the application range and application efficiency of the two substances.

Description

Technical Field

[0001] This invention relates to the field of raw material technology, and in particular to compositions containing both mussel adhesive protein and anionic polysaccharides, as well as their preparation methods and products. Background Technology

[0002] Mussel adhesive protein is a substance secreted by mussels to anchor themselves to reefs, ship bottoms, and other surfaces. Its strong wet adhesive ability attracted the attention of scientists. Further research revealed that this protein, which enables mussels to remain attached to wet surfaces for extended periods, was named "mussel adhesive protein." From its discovery and naming in 1980 to Professor Bent Samuelson's discovery of its powerful anti-inflammatory properties in 1982, numerous companies worldwide have since researched its applications. Because it was initially extracted exclusively from mussels, its production was extremely limited, earning it the nickname "marine soft gold." However, with advancements in science and technology, synthetic biology has significantly increased production, expanding its applications beyond wet adhesives.

[0003] Anionic polysaccharides are diverse, widely sourced, and have numerous applications, such as as skin feel modifiers or active ingredients. In product applications, xanthan gum, an extracellular polysaccharide derived from the fermentation of *Xanthomonas auricula-judae*, possesses unique rheological properties, good water solubility, stability to heat and acids / alkalis, and excellent compatibility with various salts. As a thickener, suspending agent, emulsifier, and stabilizer, it is widely used in over 20 industries, including food, petroleum, and pharmaceuticals, and is currently the world's largest-scale produced and most widely used microbial polysaccharide. Sclerotium tsulphureus gum, also known as sclerotium polysaccharide, is a novel nonionic biopolysaccharide polymer decomposed from the fungus *Sclerotium tsulphureus*. It possesses many excellent properties, such as high viscosity, high stability, moisturizing, anti-inflammatory, antioxidant, thickening, skin-improving, and good hydrolysis properties, and is widely used in cosmetics, pharmaceuticals, papermaking, food, and packaging. Another type of fermented high-molecular-weight polysaccharides, such as hyaluronic acid or its salts, polyglutamic acid or its salts, not only have viscosity-regulating effects, but more importantly, they have excellent skin-locking water-locking effects. Meanwhile, plant-derived polysaccharides such as β-glucan, tremella polysaccharide, and dendrobium polysaccharide all have excellent moisturizing and repairing effects.

[0004] In particular, hyaluronic acid or its salts have high clinical value and a wide range of applications. Hyaluronic acid or its salts are widely present in many parts of the human body, such as the extracellular matrix (ECM) of the skin, the eyes, and joints, playing a significant role and possessing high clinical value. Hyaluronic acid or its salts are widely used in various ophthalmic surgeries, such as lens implantation, corneal transplantation, and anti-glaucoma surgery. They can also be used to treat arthritis and accelerate wound healing. When used in cosmetics, they provide unique skin protection, keeping the skin moisturized, smooth, delicate, soft, and elastic, with anti-wrinkle, anti-aging, beauty and health benefits, and the ability to restore the skin's physiological functions.

[0005] With the development of modern medicine, more and more research results show that the deficiency of trace elements and vitamins is related to the occurrence of skin diseases. Some chemical elements exist in very small quantities in the human body, but have certain physiological functions and must be obtained through food. These are called essential trace elements. The physiological functions of trace elements are mainly: (1) essential active factors for enzymes and vitamins; (2) constituting certain hormones or participating in the action of hormones; (3) participating in nucleic acid metabolism; (4) assisting macroelements and macronutrients in their function. For example, zinc is an essential trace element for the human body. It participates in the synthesis of many enzymes in the body and the metabolism of proteins, sugars, and fats. It affects protein synthesis and wound healing and can maintain the elasticity, toughness, density, and smoothness of the skin and mucous membranes. Copper is a component of more than 30 enzymes in the human body. It participates in oxidation-reduction and tissue respiration and promotes the absorption and utilization of iron. It also has certain significance in maintaining the physiological function of the skin. Blue copper peptide is based on tripeptide-1 and chelates copper ions, thereby playing a number of skin care effects such as anti-inflammatory, repair, and firming. Other trace elements such as potassium, sodium, iron, magnesium, and selenium also play important roles in the human body.

[0006] Mussel adhesive protein is a naturally occurring positively charged protein with a molecular weight of approximately 14-100 kDa. Besides its powerful anti-inflammatory properties, it exhibits remarkable efficacy in skin repair, reducing inflammatory pigmentation, combating free radicals, and promoting film formation. Anionic polysaccharides, naturally negatively charged, have a wide range of applications.

[0007] Both of these substances are highly functional in both the medical and cosmetic / medical fields, with complementary applications and complementary functions. Many product developers and consumers hope to combine these two active ingredients in a single product for safer and more effective results, fulfilling their aspirations for a better life. However, due to their natural structural properties, it is difficult to combine them in practical applications. Forcing a combination will result in a neutralization reaction, causing flocculation and precipitation due to their relatively large molecular weights. This not only affects the appearance but also leads to the inactivation of their original efficacy.

[0008] Therefore, existing technologies still need to be improved and developed. Summary of the Invention

[0009] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition containing both mussel adhesive protein and anionic polysaccharide, as well as a preparation method and product thereof, in order to solve the compatibility problem of existing combinations of mussel adhesive protein and anionic polysaccharide.

[0010] The technical solution of the present invention is as follows:

[0011] In a first aspect, the present invention provides a composition containing both mussel adhesive protein and anionic polysaccharide, wherein, by weight, the composition comprises 0.01-0.2 parts mussel adhesive protein, 0.05-1.0 parts anionic polysaccharide, 0.3-3.0 parts inorganic salt or citric acid, and 75.0-95.0 parts water.

[0012] Optionally, by weight, the composition specifically comprises 0.01-0.2 parts of mussel adhesive protein, 0.05-1.0 parts of anionic polysaccharide, 0.3-3.0 parts of inorganic salt or citric acid, 3.0-20.0 parts of small molecule sugars, and 75.0-95.0 parts of water, wherein the number of sugar units in the small molecule sugars is less than 10.

[0013] Optionally, by weight percentage, the composition specifically comprises 0.01-0.2% mussel adhesive protein, 0.05-1.0% anionic polysaccharide, 0.3-3.0% inorganic salt or citric acid, 3.0-20.0% small molecule sugars, 1.0-3.0% preservative, 2.0-20.0% polyol, and water to 100%, wherein the small molecule sugars have less than 10 sugar units.

[0014] Optionally, the small molecule sugar is selected from at least one of mannitol, trehalose, sorbitol, xylitol, raffinose, and sucrose.

[0015] Optionally, the inorganic salt is selected from at least one of potassium salt, sodium salt, calcium salt, zinc salt, copper salt, and magnesium salt.

[0016] Optionally, the inorganic salt is selected from at least one of potassium chloride, sodium chloride, calcium chloride, zinc chloride, zinc sulfate, copper sulfate, and magnesium sulfate.

[0017] Optionally, the anionic polysaccharide is selected from at least one of hyaluronic acid or its salt, polyglutamic acid or its salt, colacid or its salt, and β-glucan.

[0018] Optionally, the molecular weight of the hyaluronic acid or its salt is 0.1-2500 kDa.

[0019] Further optionally, the hyaluronic acid or its salt has a molecular weight of 0.3-1800 kDa.

[0020] A second aspect of the present invention provides a method for preparing a composition containing both mussel adhesive protein and anionic polysaccharide, comprising the steps of: mixing mussel adhesive protein, anionic polysaccharide, inorganic salt or citric acid and water to obtain the composition.

[0021] Optionally, the step of mixing mussel adhesive protein, anionic polysaccharide, inorganic salt or citric acid and water specifically includes: adding inorganic salt or citric acid to an aqueous solution of anionic polysaccharide, and then adding mussel adhesive protein.

[0022] A third aspect of the present invention provides a product comprising the composition of the present invention containing both mussel adhesive protein and anionic polysaccharide.

[0023] Optionally, the product is a skincare product.

[0024] Further optionally, the skin care product includes one of a face mask, medical dressing patch, face cream, lotion, freeze-dried powder, serum, or toner.

[0025] Beneficial effects: This invention forms a homogeneous and transparent composition by adding inorganic salts or citric acid to an aqueous solution containing mussel adhesive protein and anionic polysaccharides. This strategy enables the compatible compounding of mussel adhesive protein and anionic polysaccharides, exhibiting long-term stability and withstanding shelf-life aging tests, thus greatly expanding the application range and efficacy of these two substances. Attached Figure Description

[0026] Figure 1 The graph shows the compatibility test results of different compositions in Example 1.

[0027] Figure 2 Images show the long-term stability of different compositions containing potassium chloride and polysaccharides in Example 1.

[0028] Figure 3 The graph shows the compatibility test results of different compositions when the sodium hyaluronate in Example 2 is a high molecular weight sodium hyaluronate.

[0029] Figure 4 The graph shows the compatibility test results of different compositions when the sodium hyaluronate in Example 2 is of medium molecular weight.

[0030] Figure 5 The graph shows the compatibility test results of different compositions when the sodium hyaluronate in Example 2 is a low molecular weight sodium hyaluronate.

[0031] Figure 6 Images show the long-term stability of different sodium chloride-containing compositions in Example 2.

[0032] Figure 7 The graph shows the compatibility test results of different compositions in Example 3.

[0033] Figure 8 Images show the long-term stability of different calcium chloride-containing compositions in Example 3.

[0034] Figure 9 The graph shows the compatibility test results of different copper sulfate compositions in Example 4.

[0035] Figure 10 The graph shows the compatibility test results of different compositions containing tripeptide-1 copper in Example 4.

[0036] Figure 11 Images showing the long-term stability of different compositions in Example 4.

[0037] Figure 12 The graph shows the compatibility test results of different compositions containing zinc sulfate in Example 5.

[0038] Figure 13 The graph shows the compatibility test results of different zinc chloride-containing compositions in Example 5.

[0039] Figure 14 Images showing the long-term stability of different compositions in Example 5.

[0040] Figure 15 The graph shows the compatibility test results of different compositions containing citric acid in Example 6.

[0041] Figure 16 The graph shows the compatibility test results of different malic acid-containing compositions in Example 6.

[0042] Figure 17 The graph shows the compatibility test results of different compositions containing glycolic acid in Example 6.

[0043] Figure 18 The graph shows the compatibility test results of different lactic acid-containing compositions in Example 6.

[0044] Figure 19 Images show the long-term stability of different compositions containing citric acid in Example 6.

[0045] Figure 20 The graph shows the changes in the moisture content of the stratum corneum of the skin in different samples tested in Example 7.

[0046] Figure 21 The graph shows the changes in transdermal water loss rate of different samples tested in Example 7.

[0047] Figure 22 This is a graph showing the changes in skin a-values ​​for different samples tested in Example 7.

[0048] Figure 23 The graph shows the changes in skin erythema index for different samples tested in Example 7.

[0049] Figure 24 The graph shows the changes in skin redness in different samples tested in Example 7.

[0050] Figure 25 This is a graph showing the change in the number of under-eye wrinkles in different samples tested in Example 7.

[0051] Figure 26 The graph shows the variation in the length of under-eye wrinkles in different samples tested in Example 7.

[0052] Figure 27 The graph shows the changes in the number of crow's feet wrinkles in different samples tested in Example 7.

[0053] Figure 28 The graph shows the variation in the length of crow's feet in different samples tested in Example 7.

[0054] Figure 29 This is a graph showing the changes in the firming and anti-wrinkle effects of different samples in Example 7.

[0055] Figure 30 This is a diagram showing the changes in eczema repair in Example 7.

[0056] Figure 31 This is a diagram showing the changes in burn repair in Example 7.

[0057] Figure 32 The graph shows the compatibility test results of different compositions in Example 8. Detailed Implementation

[0058] This invention provides a composition containing both mussel adhesive protein and anionic polysaccharides, as well as its preparation method and product. To make the objectives, technical solutions, and effects of this invention clearer and more explicit, the invention is further described in detail below. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0059] Given the natural structural properties of mussel adhesive protein and anionic polysaccharides, it is difficult to combine them for practical applications. Forcing a combination will result in a neutralization reaction, and due to their relatively large molecular weights, the mixture will flocculate and precipitate, affecting not only the appearance but also the original efficacy.

[0060] Currently, to address the compatibility issues of mussel adhesive protein and anionic polysaccharides, the common approach is to hydrolyze the mussel adhesive protein to produce amino acid fragments, thereby reducing its positive charge intensity. Furthermore, because the fragments are small, the molecular weight is reduced, making any potential neutralization reaction difficult to observe due to the extremely small molecular weight (a few amino acids). However, the structure of a substance determines its properties. Therefore, while hydrolyzing these two active ingredients is a strategy for compounding, it also inevitably results in a loss of the original functions of the substances to some extent.

[0061] Therefore, in order to solve the compatibility problem of mussel adhesive protein and anionic polysaccharides and to leverage the advantages of their combined application, research has found that by adding an appropriate amount of inorganic salt to an aqueous solution containing a certain amount of mussel adhesive protein and anionic polysaccharides (such as hyaluronic acid or its salts), a homogeneous and transparent composition can be formed.

[0062] Specifically, embodiments of the present invention provide a composition containing both mussel adhesive protein and anionic polysaccharide, wherein, by mass parts, the composition comprises 0.01-0.2 parts mussel adhesive protein, 0.05-1.0 parts anionic polysaccharide, 0.3-3.0 parts inorganic salt or citric acid, and 75.0-95.0 parts water.

[0063] This invention provides an embodiment in which a homogeneous and transparent composition is formed by adding an appropriate amount of inorganic salt or citric acid to an aqueous solution containing mussel adhesive protein and anionic polysaccharides (such as hyaluronic acid or its salts). This compounding strategy allows for the compatible compounding of mussel adhesive protein and anionic polysaccharides, exhibiting long-term stability and withstanding shelf-life aging tests, thus greatly enhancing the application range and efficacy of these two substances.

[0064] This invention, without compromising the structure of mussel adhesive protein or anionic polysaccharides, allows for the compatibility and long-term stability of two active ingredients. It solves the compatibility problem of combining the two active ingredients, expands the application range of their composition, synergistically enhances efficacy, and generates greater economic benefits.

[0065] This invention demonstrates that by adding an appropriate amount of inorganic salt to an aqueous solution containing mussel adhesive protein and anionic polysaccharides, a homogeneous and transparent composition can be formed. This is likely because, upon adding an appropriate amount of inorganic salt to the aqueous solution of the anionic polysaccharides, the metal cations of the inorganic salt aggregate in the negatively charged region of the anionic polysaccharides, forming a cationic electric field potential. Furthermore, the addition of mussel adhesive protein, which is a positively charged cationic protein, repels the cationic electric field potential surrounding the anionic polysaccharides. Therefore, in this system, the two active substances are relatively independent and unlikely to react, solving compatibility issues, expanding the application field, and improving application efficacy.

[0066] In one embodiment, the composition, by weight, specifically comprises 0.01-0.2 parts mussel adhesive protein, 0.05-1.0 parts anionic polysaccharide, 0.3-3.0 parts inorganic salt or citric acid, 3.0-20.0 parts small molecule sugars, and 75.0-95.0 parts water, wherein the small molecule sugars have less than 10 sugar units. The addition of small molecule sugars can further improve the long-term stability of the composition.

[0067] In one embodiment, the composition specifically comprises, by weight percentage, 0.01-0.2% mussel adhesive protein, 0.05-1.0% anionic polysaccharide, 0.3-3.0% inorganic salt or citric acid, 3.0-20.0% small molecule sugars, 1.0-3.0% preservative, 2.0-20.0% polyol, and water to 100%, wherein the small molecule sugars have less than 10 sugar units.

[0068] In one embodiment, the small molecule sugar is selected from at least one of mannitol, trehalose, sorbitol, xylitol, raffinose, sucrose, etc. Preferably, at least one of mannitol, trehalose, sorbitol, etc.

[0069] In one embodiment, the preservative can be a commercially available common preservative, such as methylparaben, propylparaben, phenoxyethanol, p-hydroxyacetophenone, 1,2-hexanediol, ethylhexylglycerin, sodium benzoate, etc.

[0070] In one embodiment, the inorganic salt is selected from at least one of potassium salts, sodium salts, calcium salts, zinc salts, copper salts, and magnesium salts. Preferably, it is selected from at least one of potassium chloride, sodium chloride, calcium chloride, zinc chloride, zinc sulfate, copper sulfate, and magnesium sulfate.

[0071] In one embodiment, the anionic polysaccharide is selected from at least one of hyaluronic acid or its salt, polyglutamic acid or its salt, colacid or its salt, β-glucan, etc. Preferably, it is selected from one of hyaluronic acid or its salt, colacid or its salt, β-glucan, etc.

[0072] In one embodiment, the hyaluronic acid or its salt has a molecular weight of 0.1-2500 kDa. Preferably, the molecular weight is 0.3-1800 kDa.

[0073] This invention provides a method for preparing a composition containing mussel adhesive protein and anionic polysaccharide as described above, comprising the steps of: mixing mussel adhesive protein, anionic polysaccharide, inorganic salt or citric acid and water to obtain the composition.

[0074] This invention demonstrates how adding appropriate amounts of inorganic salts or citric acid to an aqueous solution containing mussel adhesive protein and anionic polysaccharides can form a homogeneous and transparent composition. This compounding strategy allows for the compatible compounding of mussel adhesive protein and anionic polysaccharides, exhibiting long-term stability and withstanding shelf-life aging tests, thus significantly enhancing the application range and efficacy of both substances.

[0075] In one embodiment, the step of mixing mussel adhesive protein, anionic polysaccharide, inorganic salt or citric acid and water specifically includes: adding inorganic salt or citric acid to an aqueous solution of anionic polysaccharide, and then adding mussel adhesive protein.

[0076] In one specific embodiment, the step of mixing mussel adhesive protein, anionic polysaccharide, inorganic salt or citric acid and water specifically includes: adding inorganic salt to an aqueous solution of hyaluronic acid or its salt, and then adding mussel adhesive protein.

[0077] Hyaluronic acid or colacid generally exists in the form of hyaluronic acid salts (such as sodium hyaluronate) or colacid salts (such as sodium colacid). In aqueous solution, sodium hyaluronate or sodium colacid ionizes into hyaluronic acid anions or colacid anions and sodium ions (Na+). + ), the positive charge of mussel adhesive protein (NH 3+ This is mainly due to its high lysine content in its structure. Adding a certain amount of water-soluble inorganic salts, such as sodium chloride, potassium chloride, calcium chloride, zinc oxide, copper sulfate, magnesium sulfate, and zinc sulfate, to an aqueous solution of hyaluronic acid or its salts, or to an aqueous solution of colaic acid or its salts, followed by the addition of mussel adhesive protein, yields a homogeneous and transparent solution. Different amounts of these salts can extend the stability of the composition under different conditions, meeting practical application requirements.

[0078] In one embodiment, the composition comprises, by weight percentage, 0.01-0.2% mussel adhesive protein, 0.05-1.0% hyaluronic acid or its salt, 0.3-3.0% inorganic salt or citric acid, 3.0-20.0% small molecule sugars, 1.0-3.0% preservatives, 2.0-20.0% polyols, and water up to 100%;

[0079] The method for preparing the composition includes the following steps:

[0080] Mix small molecule sugars, preservatives, polyols and water, heat to 75-85℃ to completely dissolve them, and keep warm for 20-30 minutes;

[0081] After cooling to 60-70℃, add hyaluronic acid or its salt, and stir until completely dissolved.

[0082] After cooling to 50-60℃, slowly add inorganic salts or citric acid while stirring (200-500 rpm) until completely dissolved.

[0083] While stirring (200-500 rpm) at temperatures below 45°C, slowly add mussel adhesive protein to obtain a clear and transparent composition.

[0084] It should be noted that, unless otherwise specified, all percentages in this article refer to mass percentages.

[0085] In one specific embodiment, the formulation of the composition is shown in Table 1 below:

[0086] Table 1

[0087]

[0088] The method for preparing the above composition includes the following steps:

[0089] 1. Add the raw material from phase A to a beaker, heat to 75-85℃ to completely dissolve it, and keep warm for 20-30 minutes;

[0090] 2. After cooling to 60-70℃, add the raw material in phase B and stir until completely dissolved;

[0091] 3. After cooling to 50-60℃, slowly add the raw material in phase C while stirring, and stir until it is completely dissolved;

[0092] 4. While stirring (200-500 rpm) and cooling the mixture to below 45°C, slowly add the raw materials from phase D to obtain a clear and transparent composition.

[0093] This invention provides a product comprising, as described above, a composition containing both mussel adhesive protein and anionic polysaccharide.

[0094] In one embodiment, the product is a skin care product or a medical product, but is not limited to these.

[0095] In one embodiment, the skincare product includes one of a face mask, a medical dressing patch, a face cream, a lotion, a freeze-dried powder, a serum, and a toner.

[0096] In other words, the above compositions can be used to prepare skin care formulations such as face masks, medical dressings, face creams, lotions, freeze-dried powders, serums, and toners to meet different application scenarios.

[0097] The present invention will be further described in detail below through specific embodiments.

[0098] Example 1: Potassium salt group

[0099] The potassium salt group tested three potassium salts: dipotassium glycyrrhizate, potassium azeloyl diglycinate (an azeloic acid derivative), and potassium chloride. Three molecular weights of sodium hyaluronate were also tested: sodium hyaluronate with a molecular weight of 120-180 kDa (denoted as HA1), sodium hyaluronate with a molecular weight of 10-50 kDa (denoted as HA2), and sodium hyaluronate with a molecular weight of 3000-8000 Da (denoted as HA3). The stability of the compositions at different temperatures was also tested. The trade name for mussel adhesive protein is: M505C (Manufacturer: Shenzhen Baiyin Biotechnology Co., Ltd., Mussel Adhesive Protein Content: 5000ppm).

[0100] Preliminary compatibility test: HA1 was selected for testing to determine whether the two substances are compatible in the absence of inorganic salts.

[0101] The experimental formula is shown in Table 2 below:

[0102] Table 2

[0103]

[0104] The results above indicate that only the inorganic salt potassium chloride can promote the compatibility between mussel adhesive protein and sodium hyaluronate.

[0105] Further stability testing of the potassium chloride-compatible system: Potassium chloride can promote the compatibility of mussel adhesive protein with large molecular weight sodium hyaluronate, but only systems that are compatible under different accelerated aging conditions over a long period of time are systems with application value.

[0106] The experimental formulas are shown in Table 3 below:

[0107] Table 3

[0108]

[0109] The long-term (3 months) stability (aging conditions: 50℃, 38℃, 25℃, 4℃) results of the above samples are shown in Table 4 below:

[0110] Table 4

[0111]

[0112] The above test results show that, with the synergistic effect of inorganic salt potassium chloride and small molecule sugars, mussel adhesive protein can be compatible with sodium hyaluronate of different molecular weights for a long time. The metal ions contained therein not only promote the compatibility of the two active substances, but also achieve long-term stability.

[0113] Example 2: Sodium salt group

[0114] The sodium salt group tested three sodium salts: sodium chloride, disodium hydrogen phosphate, and sodium dihydrogen phosphate. Three molecular weights of sodium hyaluronate were also tested: sodium hyaluronate with a molecular weight of 120-180 kDa (denoted as HA1), sodium hyaluronate with a molecular weight of 10-50 kDa (denoted as HA2), and sodium hyaluronate with a molecular weight of 3000-8000 Da (denoted as HA3). The stability of the compositions at different temperatures was also tested. The trade name for mussel adhesive protein is: M505C (Manufacturer: Shenzhen Baiyin Biotechnology Co., Ltd., Mussel protein content: 5000ppm).

[0115] Preliminary compatibility test: HA1 was selected for testing to determine whether the two substances are compatible in the absence of inorganic salts.

[0116] The experimental formulas are shown in Table 5 below:

[0117] Table 5

[0118]

[0119] The results above indicate that only the inorganic salt sodium chloride can promote the compatibility of mussel adhesive protein with high molecular weight sodium hyaluronate, while the other two common inorganic sodium salts do not have this function.

[0120] Preliminary compatibility test: HA2 was selected for testing to see if the two substances are compatible in the absence of inorganic salts.

[0121] The experimental formula is shown in Table 6 below:

[0122] Table 6

[0123]

[0124] The results above indicate that only the inorganic salt sodium chloride can promote the compatibility of mussel adhesive protein with medium molecular weight sodium hyaluronate, while the other two common inorganic sodium salts do not have this function.

[0125] Preliminary compatibility test: HA3 was selected for testing to determine whether the two substances are compatible in the absence of inorganic salts.

[0126] The experimental formula is shown in Table 7 below:

[0127] Table 7

[0128]

[0129]

[0130] The results above indicate that only the inorganic salt sodium chloride can promote the compatibility of mussel adhesive protein with low molecular weight sodium hyaluronate, while the other two common inorganic sodium salts do not have this function.

[0131] Further stability tests of the sodium chloride-compatible systems (samples 12, 15, and 18): Sodium chloride can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights, but only systems that are compatible under different accelerated aging conditions over a long period of time are systems with application value.

[0132] The long-term (3 months) stability (aging conditions: 50℃, 38℃, 25℃, 4℃) results of the above samples are shown in Table 8 below:

[0133] Table 8

[0134]

[0135] The above test results show that, with the synergistic effect of inorganic salt sodium chloride, mussel adhesive protein can be compatible with sodium hyaluronate of different molecular weights for a long time. The metal ions contained therein not only promote the compatibility of the two active substances, but also achieve long-term stability.

[0136] Example 3: Calcium Salt Group

[0137] The calcium salt group tested one calcium salt: calcium chloride, and three molecular weights of sodium hyaluronate: sodium hyaluronate with a molecular weight of 120-180 kDa (denoted as HA1), sodium hyaluronate with a molecular weight of 10-50 kDa (denoted as HA2), and sodium hyaluronate with a molecular weight of 5000-8000 Da (denoted as HA3). The stability of the composition at different temperatures was also tested. The trade name for mussel adhesive protein is: M505C (Manufacturer: Shenzhen Baiyin Biotechnology Co., Ltd., Mussel protein content: 5000ppm).

[0138] Preliminary compatibility test: Test whether calcium chloride promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0139] The experimental formula is shown in Table 9 below:

[0140] Table 9

[0141]

[0142] The results above indicate that inorganic calcium chloride can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0143] Further stability tests of the calcium chloride-compatible system (samples 19, 20, 21): Calcium chloride can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights, but only systems that are compatible under different accelerated aging conditions over a long period of time are systems with application value.

[0144] The long-term (3 months) stability (aging conditions: 50℃, 38℃, 25℃, 4℃) results of the above samples are shown in Table 10 below:

[0145] Table 10

[0146]

[0147] The above test results show that, with the synergistic effect of inorganic salt calcium chloride, mussel adhesive protein can be compatible with low- to medium-molecular-weight sodium hyaluronate for a long time. The metal ions contained therein not only promote the compatibility of the two active substances, but also achieve long-term stability.

[0148] Example 4: Copper Salt Group

[0149] The copper salt group tested two copper salts: copper sulfate and copper tripeptide-1. Three molecular weights of sodium hyaluronate were also tested: sodium hyaluronate with a molecular weight of 120-180 kDa (denoted as HA1), sodium hyaluronate with a molecular weight of 10-50 kDa (denoted as HA2), and sodium hyaluronate with a molecular weight of 5000-8000 Da (denoted as HA3). The stability of the compositions at different temperatures was also tested. The trade name for mussel adhesive protein is: M505C (Manufacturer: Shenzhen Baiyin Biotechnology Co., Ltd., Mussel protein content: 5000ppm).

[0150] Preliminary compatibility test: Test whether copper sulfate promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0151] The experimental formula is shown in Table 11 below:

[0152] Table 11

[0153]

[0154] The above results indicate that inorganic copper sulfate can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0155] Preliminary compatibility testing: Testing whether tripeptide-1 copper promotes compatibility between mussel adhesive protein and sodium hyaluronate (HA1-HA2).

[0156] The experimental formula is shown in Table 12 below:

[0157] Table 12

[0158]

[0159]

[0160] The above results indicate that tripeptide-1 copper cannot promote the compatibility of mussel adhesive protein with medium and high molecular weight sodium hyaluronate. The possible reason is that copper ions are chelated by tripeptide-1 and fail to be released into the system, thus resulting in a weak improvement effect.

[0161] Further stability tests of the copper sulfate-compatible system (samples 22, 23, 24, 27): Copper sulfate can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights, but only systems that are compatible under different accelerated aging conditions over a long period of time are systems with application value.

[0162] The long-term (3 months) stability (aging conditions: 50℃, 38℃, 25℃, 4℃) results of the above samples are shown in Table 13 below:

[0163] Table 13

[0164]

[0165] The above test results indicate that, with the synergistic effect of inorganic copper sulfate or tripeptide-1 copper, mussel adhesive protein can maintain long-term compatibility with low- to medium-molecular-weight sodium hyaluronate, and exhibits even better compatibility with low-molecular-weight sodium hyaluronate. The contained metal ions not only promote compatibility between the two active ingredients but also contribute to long-term stability.

[0166] Example 5: Zinc Salt Group

[0167] Four candidate zinc salts were selected for the zinc salt group: zinc sulfate, zinc chloride, zinc PCA, and zinc gluconate. Based on the experimental results of the compatibility of tripeptide-1 copper with two active ingredients, if the metal ions are in a chelated state, it may limit their compatibility with the system. Therefore, only the effects of zinc sulfate and zinc chloride (with auxiliary trace acid) were tested. Three molecular weights of sodium hyaluronate were tested: sodium hyaluronate with a molecular weight of 120-180 kDa (denoted as HA1), sodium hyaluronate with a molecular weight of 10-50 kDa (denoted as HA2), and sodium hyaluronate with a molecular weight of 5000-8000 Da (denoted as HA3). The stability of the compositions at different temperatures was also tested. The trade name for mussel adhesive protein is: M505C (Manufacturer: Shenzhen Baiyin Biotechnology Co., Ltd., Mussel protein content: 5000ppm).

[0168] Preliminary compatibility testing: Testing whether zinc sulfate promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0169] The experimental formula is shown in Table 14 below:

[0170] Table 14

[0171]

[0172] The above results indicate that inorganic zinc sulfate can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0173] Preliminary compatibility testing: Testing whether zinc chloride promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0174] The experimental formulas are shown in Table 15 below:

[0175] Table 15

[0176]

[0177] The results above indicate that inorganic zinc chloride can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0178] Further stability tests of the zinc sulfate and zinc chloride compatible system (samples 28-33): The two zinc salts can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights, but only the system that is compatible under different accelerated aging conditions over a long period of time is a system with application value.

[0179] The long-term (3 months) stability (aging conditions: 50℃, 38℃, 25℃, 4℃) results of the above samples are shown in Table 16 below:

[0180] Table 16

[0181]

[0182]

[0183] The above test results indicate that, with the synergistic effect of inorganic zinc chloride, mussel adhesive protein can maintain long-term compatibility with sodium hyaluronate of different molecular weights; compared to zinc sulfate, zinc chloride exhibits better long-term stability and compatibility. The metal ions it contains not only promote compatibility between the two active ingredients but also contribute to long-term stability.

[0184] Example 6: Fruit Acid Group

[0185] Five candidate acids were identified in the fruit acid group: citric acid, malic acid, tartaric acid, glycolic acid, and lactic acid. Three molecular weights of sodium hyaluronate were tested: 120-180 kDa (HA1), 10-50 kDa (HA2), and 5000-8000 Da (HA3). The stability of the composition at different temperatures was also assessed. The trade name for mussel adhesive protein is: M505C (Manufacturer: Shenzhen Baiyin Biotechnology Co., Ltd., Mussel protein content: 5000ppm).

[0186] Preliminary compatibility test: Test whether citric acid promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0187] The experimental formula is shown in Table 17 below:

[0188] Table 17

[0189]

[0190] The results above indicate that citric acid can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0191] Preliminary compatibility test: Test whether malic acid promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0192] The experimental formula is shown in Table 18 below:

[0193] Table 18

[0194]

[0195] The results above indicate that malic acid cannot promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0196] Preliminary compatibility testing: Testing whether glycolic acid promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0197] The experimental formula is shown in Table 19 below:

[0198] Table 19

[0199]

[0200] The results above indicate that glycolic acid cannot promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0201] Preliminary compatibility test: Test whether lactic acid promotes the compatibility of mussel adhesive protein with sodium hyaluronate (HA1-HA3).

[0202] The experimental formula is shown in Table 20 below:

[0203] Table 20

[0204]

[0205]

[0206] The results above indicate that lactic acid cannot promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights.

[0207] Further stability tests of the citric acid-compatible system (samples 34-36): Citric acid can promote the compatibility of mussel adhesive protein with sodium hyaluronate of different molecular weights, but only systems that are compatible under different accelerated aging conditions over a long period of time are systems with application value.

[0208] The long-term (3-month) stability (aging conditions: 50℃, 38℃, 25℃, 4℃) results are shown in Table 21 below:

[0209] Table 21

[0210]

[0211] The test results above indicate that, with the synergistic effect of citric acid, mussel adhesive protein can maintain long-term compatibility with sodium hyaluronate of different molecular weights.

[0212] Example 7: Comparison of repair effects on sensitive skin (potassium and sodium groups)

[0213] Four groups of samples, including the compositions from Examples 1 and 2 (i.e., samples 4, 5, and 12) and a placebo, were selected and compared in a sensitive skin population for efficacy tests in moisturizing, reducing transepidermal water loss, and reducing redness. The specific test formulations are shown in Table 22 below.

[0214] Table 22

[0215]

[0216]

[0217] Preparation method:

[0218] Step 1: Heat phase A and phase B to 80℃ respectively, stir (300 rpm) to dissolve evenly, and keep warm for 25 min; add phase B to phase A, stir (400 rpm) to emulsify, homogenize (2500 rpm) for 2 min, vacuum defoam, and then cool down;

[0219] Step 2: Cool down to about 60℃, add phase C, and stir (250 rpm) until homogeneous;

[0220] Step 3: Cool down to below 45℃, add phase D, and stir (250 rpm) until homogeneous;

[0221] Step 4: Conduct relevant physicochemical tests, and repackage the products after they pass the tests.

[0222] Note: The placebo component consists of two products, A and B (product A contains an equal amount of mussel adhesive protein, and product B contains an equal amount of sodium hyaluronate).

[0223] The experimental test information is as follows:

[0224] Test objective: Evaluation of the human efficacy of cosmetics.

[0225] Test items: Repair, suitable for sensitive skin

[0226] Reference standards: QB / T 4256-2011 Guidelines for evaluating the moisturizing efficacy of cosmetics; T / GDCA

[0227] 009-2022 Human Evaluation Method for Repair Efficacy; T / CNMIA0015-2020 Clinical Evaluation Standard for Soothing Skincare Products

[0228] Test participants: Regardless of gender, aged 18-60, those with lactic acid stinging sensation were selected as having sensitive skin.

[0229] Test area: face

[0230] Test environment: Temperature 21℃±1℃; Humidity 50%±10%RH

[0231] Testing instruments: Stratum corneum moisture content meter; Transepidermal water loss meter; C-cube imaging device

[0232] Follow-up visits: 0 days, 3 days, 7 days, 14 days, and 28 days after product use.

[0233] Negative records: None

[0234] Sensitive Skin Population Screening Test: This test screened 15 individuals with sensitive skin to participate in the sample efficacy evaluation using the following method.

[0235] Sample usage instructions: After cleansing in the morning and evening, apply an appropriate amount of product evenly to the face and gently massage until absorbed.

[0236] Lactic acid sting test:

[0237] Take 50 μl of 10% lactic acid solution and drop it onto a 1*1 cm piece of non-woven fabric. Apply this fabric to the nasolabial fold on one side of the subject, with distilled water used as a control on the other side. The tester inquires about the subject's stinging sensation at 0.5, 2.5, and 5 minutes, and scores it using the "4-point method" in the table below. Subjects with a cumulative stinging sensation ≥3 points on the lactic acid side relative to the distilled water side are considered to have lactic acid stinging and can be classified as having sensitive skin. The lactic acid stinging scoring levels are shown in Table 23 below:

[0238] Table 23

[0239]

[0240] The moisturizing effects were compared by measuring the increase in the water content of the stratum corneum. The results were as follows: Figure 20As shown, the significance level is indicated by the following methods: "ns" indicates no statistical difference (p≥0.05); p<0.05 indicates a significant difference; "*" indicates 0.01≤p<0.05; "**" indicates 0.001≤p<0.01; "***" indicates p<0.001. Compared with day D0, samples 4, 5, and 12, as well as the placebo group, significantly increased skin hydration at days 3, 7, 14, and 28, indicating that the samples have a repairing effect on the skin. This suggests that products containing mussel adhesive protein and sodium hyaluronate can improve skin hydration in sensitive skin. Compared with the placebo group, samples 4, 5, and 12 showed a greater increase in skin hydration at the same time points than the placebo group, indicating that the addition of relevant metallic components in the composition not only benefits the combination of the two classic ingredients but also synergistically enhances moisturizing effects, contributing to the care of sensitive skin.

[0241] The repair efficacy was compared by measuring the reduction in transdermal water loss rate. The results were as follows: Figure 21 The significance level was indicated by the following methods: "ns" indicates no statistical difference (p≥0.05); p<0.05 indicates a significant difference; "*" indicates 0.01≤p<0.05; "**" indicates 0.001≤p<0.01; and "***" indicates p<0.001. Compared to day D0, samples 4, 5, and 12, as well as the placebo group, significantly reduced transepidermal water loss at day 28, indicating a skin-repairing effect. This suggests that products containing mussel adhesive protein and sodium hyaluronate can reduce transepidermal water loss in sensitive skin. Compared to the placebo group, samples 4, 5, and 12 showed a greater reduction in transepidermal water loss than the placebo group, indicating that the addition of relevant metallic components to the composition not only facilitates the synergistic effect of the two classic ingredients but also enhances the repair effect on sensitive skin and strengthens the skin barrier function.

[0242] A comparison of redness-reducing effects was conducted using the decrease in skin redness a value and erythema index E value as indicators. The results are as follows: Figure 22-23 The significance level was indicated by the following methods: "ns" indicates no statistical difference (p≥0.05); p<0.05 indicates a significant difference; "*" indicates 0.01≤p<0.05; "**" indicates 0.001≤p<0.01; "***" indicates p<0.001. Compared to day D0, samples 4, 5, and 12, as well as the placebo group, significantly reduced the skin redness a value and erythema index E value at day 28, indicating that the samples had a redness-reducing effect on the skin. This suggests that products containing mussel adhesive protein and sodium hyaluronate can alleviate skin redness in sensitive skin. Compared to the placebo group, samples 4, 5, and 12 showed a greater reduction in skin a value and E value than the placebo group, indicating that the addition of relevant metallic components to the composition not only facilitates the synergistic effect of the two classic ingredients but also enhances the repair effect on sensitive skin and strengthens the skin barrier function.

[0243] Four groups of samples (samples 9, 23, and 27) and a placebo were selected for efficacy comparison testing. The specific test formulas were the same as those in Table 22, with the only difference being that samples 9, 23, and 27 were used respectively. The soothing efficacy comparison was represented by images, and the results are shown below. Figure 24 As shown in the figure. Compared to day D0, samples 9, 23, and 27, as well as the placebo group, all showed a significant reduction in skin redness on day 28, indicating that the samples have a soothing effect on the skin. This suggests that products containing mussel adhesive protein and sodium hyaluronate can alleviate skin redness in sensitive skin. Compared to the placebo group, samples 9, 23, and 27 showed better improvement in skin redness, indicating that the addition of relevant metallic components to the composition not only benefits the synergistic effect of the two classic ingredients but also enhances the repair effect on sensitive skin and strengthens the skin barrier function.

[0244] The experimental test information is as follows:

[0245] Test objective: Evaluation of the human efficacy of cosmetics.

[0246] Test items: Suitable for firming and sensitive skin

[0247] Reference Standard: T / ZHCA006-2019 Test Method for Anti-wrinkle Efficacy of Cosmetics

[0248] Test participants: Regardless of gender, aged 30-65, those with lactic acid stinging sensation were selected as having sensitive skin.

[0249] Test area: face

[0250] Test environment: Temperature 21℃±1℃; Humidity 50%±10%RH

[0251] Testing instrument: Antera 3D

[0252] Follow-up visits: 0 days, 3 days, 7 days, 14 days, and 28 days after product use.

[0253] Negative records: None

[0254] Sensitive Skin Population Screening Test: This test screened 15 individuals with sensitive skin to participate in the sample efficacy evaluation using the following method;

[0255] Sample usage instructions: After cleansing in the morning and evening, apply an appropriate amount of product evenly to the face and gently massage until absorbed.

[0256] Lactic acid sting test:

[0257] Take 50 μl of 10% lactic acid solution and drop it onto a 1*1 cm piece of non-woven fabric. Apply this fabric to the nasolabial fold on one side of the subject, with distilled water used as a control on the other side. The tester asks the subject about their stinging sensation at 0.5, 2.5, and 5 minutes, and scores them using the "4-point method" in the table below. Those with a cumulative stinging sensation ≥3 points on the lactic acid side relative to the distilled water side are considered to have lactic acid stinging and can be classified as having sensitive skin. The lactic acid stinging scoring levels are shown in Table 24 below:

[0258] Table 24

[0259]

[0260]

[0261] The firming effect comparison was conducted by evaluating the number and length of under-eye wrinkles. The results are as follows: Figure 25-26 The significance level was indicated by the following methods: "ns" indicates no statistical difference (p ≥ 0.05); "p < 0.05" indicates a significant difference; "*" indicates 0.01 ≤ p < 0.05; "**" indicates 0.001 ≤ p < 0.01; and "***" indicates p < 0.001. Compared to day D0, samples 27, 23, and 9, as well as the placebo group, significantly reduced the number of under-eye wrinkles at days 7 and 28. Compared to the placebo group, sample 27 showed better improvement, while the other two groups showed better results than the placebo group at different time points. Compared to day D0, samples 27, 23, and 9 significantly reduced the length of under-eye wrinkles at days 7, 14, and 28, showing better improvement compared to the placebo group. These results indicate that the samples containing active ingredients have a skin-tightening effect. The addition of relevant metallic components to the composition not only facilitates the synergistic effect of the two classic ingredients but also enhances moisturizing efficacy, contributing to the firming care of sensitive skin.

[0262] The firming effect was compared and evaluated by the number and length of wrinkles at the corners of the eyes. The results are as follows: Figure 27-28 As shown; where the significance level is indicated by the following methods: "ns" indicates no statistical difference (p≥0.05); p<0.05 indicates a significant difference; "*" indicates 0.01≤p<0.05; "**" indicates 0.001≤p<0.01; "***" indicates p<0.001. Compared with day D0, sample 27 significantly reduced the number of crow's feet wrinkles at days 14 and 28, which was better than the placebo group. Compared with day D0, samples 27, 23, and 9 significantly reduced the length of crow's feet wrinkles at days 3, 7, 14, and 28, which was better than the placebo group, indicating that the samples have a skin-tightening effect. This indicates that the addition of relevant metallic components to the composition not only benefits the combination of the two classic ingredients but also synergistically enhances the moisturizing effect, contributing to the firming care of sensitive skin.

[0263] A comparison of firming effects was conducted using real-life case photos of under-eye and crow's feet wrinkles to evaluate the effectiveness. The results are as follows: Figure 29 As shown in the figure. Compared to day D0, samples 27, 23, and 9, as well as the placebo group, significantly increased skin hydration at days 3, 7, 14, and 28, indicating that the samples have a repairing effect on the skin. This suggests that products containing mussel adhesive protein and sodium hyaluronate can improve skin hydration in sensitive skin. Compared to the placebo group, samples 27, 23, and 9 showed a greater increase in skin hydration at the same time points, indicating that the addition of relevant metallic components to the composition not only benefits the synergistic effect of the two classic ingredients but also enhances their moisturizing efficacy, thus contributing to the care of sensitive skin.

[0264] Example Sample 19: Efficacy in eczema / wound repair

[0265] Apply the product to the affected area; it should heal within 3-5 days. Figures 30-31 As can be seen.

[0266] Example 8:

[0267] Different anionic polysaccharides were selected for testing to determine whether mussel adhesive protein could be compatible with different anionic polysaccharides in the absence of inorganic salts. The experimental formulations are shown in Table 25 below.

[0268] Table 25

[0269]

[0270]

[0271] From Table 25 and Figure 32 The results showed that some anionic polysaccharides can be compatible with mussel adhesive protein in combination, while the improvement effect of thickening anionic polysaccharides (xanthan gum, sclerotium gum, etc.) was not good. Combining mussel adhesive protein with some anionic polysaccharides can enhance their respective application range and efficacy.

[0272] In summary, this invention provides a composition containing both mussel adhesive protein and anionic polysaccharides, along with its preparation method and product. This invention achieves a homogeneous and transparent composition by adding inorganic salts or citric acid to an aqueous solution containing mussel adhesive protein and anionic polysaccharides. This strategy enables the compatible combination of mussel adhesive protein and anionic polysaccharides, exhibiting long-term stability and withstanding shelf-life aging tests, significantly enhancing the application range and efficacy of these two substances.

[0273] It should be understood that the application of the present invention is not limited to the examples above. Those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims.

Claims

1. A method for compatibly combining mussel adhesive protein with anionic polysaccharides, characterized in that, The method includes the following steps: Mix small molecule sugars, preservatives, polyols and water, heat to 75-85℃ to completely dissolve them, and keep warm for 20-30 minutes; After cooling to 60-70℃, add the anionic polysaccharide and stir until completely dissolved; After cooling to 50-60℃, add the inorganic salt while stirring, and stir until completely dissolved. Cool the mixture to below 45°C and add mussel adhesive protein while stirring to obtain a clear and transparent composition; The composition, by weight percentage, specifically comprises 0.01-0.2% mussel adhesive protein, 0.05-1.0% anionic polysaccharide, 0.3-3.0% inorganic salt, 3.0-20.0% small molecule sugar, 1.0-3.0% preservative, 2.0-20.0% polyol, and water to 100%, wherein the small molecule sugar has less than 10 sugar units; The small molecule sugar is selected from two of mannitol and trehalose, the inorganic salt is selected from potassium chloride, and the anionic polysaccharide is selected from hyaluronic acid or its salt. Alternatively, the inorganic salt is selected from sodium chloride, and the anionic polysaccharide is selected from hyaluronic acid or its salt, wherein the molecular weight of the hyaluronic acid or its salt is 120-180 kDa or 3000-8000 kDa. Alternatively, the inorganic salt is selected from copper sulfate, and the anionic polysaccharide is selected from hyaluronic acid or its salt, wherein the molecular weight of the hyaluronic acid or its salt is 3000-8000 kDa; Alternatively, the inorganic salt may be selected from zinc chloride, and the anionic polysaccharide may be selected from hyaluronic acid or its salt.

2. A composition containing both mussel adhesive protein and anionic polysaccharide, characterized in that, The mixture is obtained by compounding using the method described in claim 1.

3. A method for compatibility between mussel adhesive protein and anionic polysaccharide substances, characterized in that, The method includes the following steps: Mix small molecule sugars, preservatives, polyols and water, heat to 75-85℃ to completely dissolve them, and keep warm for 20-30 minutes; After cooling to 60-70℃, add the anionic polysaccharide and stir until completely dissolved; After cooling to 50-60℃, add citric acid while stirring until completely dissolved. Cool the mixture to below 45°C and add mussel adhesive protein while stirring to obtain a clear and transparent composition; The composition, by weight percentage, specifically comprises 0.01-0.2% mussel adhesive protein, 0.05-1.0% anionic polysaccharide, 0.3-3.0% citric acid, 3.0-20.0% small molecule sugars, 1.0-3.0% preservative, 2.0-20.0% polyol, and water to 100%, wherein the number of sugar units in the small molecule sugars is less than 10; The anionic polysaccharide is selected from hyaluronic acid or its salt, and the molecular weight of hyaluronic acid or its salt is 0.1-2500 kDa. The small molecule sugar is selected from at least one of mannitol, trehalose, sorbitol, xylitol, raffinose, and sucrose.

4. A composition containing both mussel adhesive protein and anionic polysaccharide, characterized in that, The mixture is obtained by compounding using the method described in claim 3.

5. A product characterized in that, The product includes the composition according to claim 2 or 4 that contains both mussel adhesive protein and anionic polysaccharide.

Images