Organic acid and chelated copper compounded cow hoof bath disinfectant and preparation method thereof

Abstract

The invention discloses a dairy cow hoof bath disinfectant compounded by organic acid and chelated copper. The dairy cow hoof bath disinfectant is prepared from, by mass, 1-30 parts of organic acid, 1-5 parts of chelated zinc, 1-5 parts of chelated copper, 1-5 parts of chelated manganese, 10-40 parts of surfactant and 0.01-1 part of coloring agent. The invention also discloses a preparation method of the organic acid and chelated copper compounded dairy cow hoof bath disinfectant, which comprises the following steps: at room temperature, dissolving the coloring agent in water for later use, adding the organic acid and chelated copper into a container, and uniformly stirring to obtain the organic acid and chelated copper compounded dairy cow hoof bath disinfectant. And sequentially adding the chelated zinc, the chelated manganese, the anionic surfactant, the nonionic surfactant and the coloring agent while stirring, and dissolving. Finally, purified water is added to 100 ml, stirring is conducted till the liquid does not contain solid mixtures, foams and the like, and the compound hoof bath disinfectant is obtained and has good adhesion and permeability, is safe and efficient, does not contain any toxic substance residues and is used for hoof bath disinfection without antibiotics.

Description

Technical Field

[0001] The invention relates to the technical field of cow hoof disinfection, and in particular to an organic acid and chelated copper compound cow hoof bath disinfectant and a preparation method thereof. Background Art

[0002] Hoof disease causes huge losses to the ranch, including decreased milk production, decreased fertility, secondary diseases, premature culling, treatment costs, and decreased cow welfare. Heat stress, parturition stress, and environmental humidity all have an impact on hoof disease in dairy cows. The hoof of the hind limbs of dairy cows is under a heavy burden, so the outer hoof of the hind limbs is easily damaged, becoming the main site of hoof disease. Hoof disease in dairy cows not only affects feed intake and leads to decreased milk production, but may also lead to premature culling, causing significant losses to the dairy farming industry. The market urgently needs to develop natural hoof care products that do not contain antibiotic ingredients, such as hoof creams and hoof baths. For hoof health problems, hoof bathing is the most commonly used hoof care method in farms, and hoof trimming, drug spraying, and ointment application are also common in the prevention and treatment of hoof diseases. Currently, the widely used hoof baths include formaldehyde solution, copper sulfate solution, and antibiotics. However, these traditional hoof baths each have some disadvantages. For example, formaldehyde solution is highly irritating and can damage the health of cattle hooves when used; copper sulfate solution can pollute the environment; antibiotics can easily cause drug resistance, etc.

[0003] Hoof bath is an important hoof care product for dairy cows. It is suitable for group prevention and clinical treatment. Before use, fill the hoof bath with clean water to clean the cow's hooves 2-3 times to keep them clean. Then, fill the hoof bath with the prepared hoof bath and let the ranch cattle pass through to prevent hoof diseases. Hoof baths usually contain adhesives with good adhesion, which can prolong the duration of drug action. For severe cases, clinical treatment should be carried out, and after the treatment, the cow's hooves should be re-bathed. Therefore, hoof bath is an essential product for the prevention and treatment of hoof diseases in dairy cows.

[0004] In summary, it is an urgent need for the majority of farmers to prepare a hoof bath disinfectant that can treat various types of hoof diseases in dairy cows, has good adhesion and permeability, is safe and efficient, has no toxic residues, and does not use antibiotics. To this end, we proposed a dairy cow hoof bath disinfectant composed of an organic acid and chelated copper and a preparation method thereof to solve the above problems. Summary of the invention

[0005] The present invention provides a cow hoof bath disinfectant compounded with organic acid and chelated copper and a preparation method thereof, which solves the defects of existing cow hoof bath disinfectants and creates a solution with significant bactericidal effect and no irritation to cow skin. The main raw material components are organic acids with anti-inflammatory and antioxidant properties and chelated copper with significant antibacterial effect. In addition, through scientific compounding, auxiliary ingredients such as chelated zinc, chelated manganese, anionic surfactants, non-ionic surfactants and colorants are added to improve the cow's own disease resistance. This formula not only has strong foaming ability and good bactericidal performance, but also has excellent adsorption capacity and stability, can effectively adhere to the cow's hoof, reduce drug loss, and thus reduce costs.

[0006] The invention provides an organic acid and chelated copper compound dairy cow hoof bath disinfectant, comprising the following components in parts by mass: 1-30 parts of organic acid, 1-5 parts of chelated zinc, 1-5 parts of chelated copper, 1-5 parts of chelated manganese, 10-40 parts of surfactant and 0.01-1 part of colorant.

[0007] Preferably, the organic acid comprises 25 parts, chelated zinc comprises 1.1 parts, chelated copper comprises 1 part, chelated manganese comprises 1.2 parts, anionic surfactant comprises 22.5 parts, nonionic surfactant comprises 10 parts, and colorant comprises 0.01 parts.

[0008] Preferably, the organic acid is any one or more of caprylic acid, formic acid, L-lactic acid, capric acid, glycolic acid, L-malic acid and mandelic acid.

[0009] Preferably, the chelated zinc is any one or more of zinc oxalate, zinc lactate, zinc citrate, and glycine chelated zinc.

[0010] Preferably, the chelated copper is any one or more of citrate chelated copper, copper citrate, and copper glycinate.

[0011] Preferably, the anionic surfactant is any one or more of sodium α-olefin sulfonate, sodium dioctyl sulfosuccinate, sodium lauryl ether sulfate, sodium dodecylbenzene sulfonate and sodium dodecyl sulfate.

[0012] Preferably, the nonionic surfactant is any one or more of C9-11 polyether-8, alcohol ether glycoside and coconut oil fatty acid diethanolamide.

[0013] Preferably, the colorant is any one or more of brilliant blue, fruit green and lemon yellow.

[0014] The invention also discloses a method for preparing a dairy cow hoof bath disinfectant compounded with organic acid and chelated copper, comprising the following steps: firstly dissolving a colorant in water at room temperature for standby use, then adding an organic acid and chelated copper into a container, stirring evenly, then sequentially adding chelated zinc, chelated manganese, anionic surfactant, nonionic surfactant, and colorant while stirring to dissolve. Finally, adding purified water, stirring until the liquid does not contain solid mixture, foam, etc., to obtain a compound hoof bath disinfectant.

[0015] It can be seen from the above technical scheme that the present invention provides a dairy cow hoof bath disinfectant compounded with organic acid and chelated copper and a preparation method thereof.

[0016] Compared with the prior art, the beneficial effect of the present invention is to overcome the defects of the existing cow hoof bath disinfectants and create a solution with significant bactericidal effect and no irritation to the cow's skin. The main raw material components use organic acids with anti-inflammatory and antioxidant properties and chelated copper with significant antibacterial effects. In addition, through scientific compounding, auxiliary ingredients such as chelated zinc, chelated manganese, anionic surfactants, non-ionic surfactants and colorants are added to improve the cow's own disease resistance. This formula not only has strong foaming ability and good bactericidal properties, but also has excellent adsorption and stability, can effectively adhere to the cow's hoof, reduce drug loss, and thus reduce costs. DETAILED DESCRIPTION

[0017] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0018] Example 1

[0019] A dairy cow hoof bath disinfectant compounded with organic acid and chelated copper, wherein anionic surfactants and nonionic surfactants are screened.

[0020] Preferably, the anionic surfactant is any one or more of sodium α-olefin sulfonate, sodium octyl sulfosuccinate, sodium lauryl ether sulfate, sodium dodecylbenzene sulfonate and sodium dodecyl sulfate.

[0021] Preferably, the nonionic surfactant is any one or more of C9-11 polyether-8, alcohol ether glycoside and coconut oil fatty acid diethanolamide.

[0022] As an auxiliary material of disinfectant, anionic surfactant has strong cleaning, emulsification, wetting, solubilization and foaming properties, high biodegradability and low irritation. Its wide application in cleaning products proves that it can produce stable foam, cover the surface to form a protective film, and effectively clean the hoof and prevent bacterial growth in hoof bath disinfection. Its strong adsorption capacity means that it can improve the adsorption effect of disinfectants and reduce costs. At the same time, it is environmentally friendly and sustainable. Its comprehensive advantages enable it to have excellent performance in the field of disinfectants. Among them, sodium α-olefin sulfonate and sodium dodecyl ether sulfate have better foaming properties, and sodium dodecylbenzene sulfonate and sodium α-olefin sulfonate have better wetting ability. Sodium octyl succinate sulfonate has high penetration, fast and uniform permeability, and good lubricity, emulsification and foaming properties.

[0023] Preferably, sodium dodecyl ether sulfate / sodium dodecylbenzene sulfonate is compounded with sodium α-olefin sulfonate and sodium octyl sulfosuccinate to improve the foaming performance, wetting power and penetration power of the product, as well as environmental protection, safety and low irritation.

[0024] Nonionic surfactants have significant advantages as auxiliary materials for hoof bath disinfectants. Not only does the non-ionization property in water give it stability, but when compounded with anionic surfactants, it can also enhance interfacial interactions, improve stability, and effectively reduce surface tension. In addition, the green and degradable properties of certain nonionic surfactants make them an environmentally friendly choice. In the compound system, nonionic surfactants also exhibit excellent flocculation, thickening and other properties, providing assistance when using hoof bath disinfectants.

[0025] While keeping the organic acid component at 15 parts unchanged, the anionic surfactants were formulated in different proportions according to Table 1, and the foaming performance was evaluated. The screening results are shown in Table 2.

[0026] Table 1 Anionic surfactant screening ratio table

[0027]

[0028] Foaming performance test: Take 1 mL of each prepared sample, add 49 mL of water to a beaker and mix well, place on a high-speed stirrer at 1000 rpm for 5 minutes. The foaming performance is comprehensively evaluated by the foam height, fineness (the number of bubbles within a length of 10 mm on the same plane is used to count the foam diameter, mm) and defoaming time.

[0029] Table 2 Anionic surfactant foaming performance results

[0030]

[0031] Preferably, when sodium dodecyl ether sulfate: sodium α-olefin sulfonate: sodium octyl sulfosuccinate = 1:2.5:1 or sodium dodecylbenzene sulfonate: sodium α-olefin sulfonate: sodium octyl sulfosuccinate = 1:2.5:1, the foaming performance of the final product is good.

[0032] Similarly, the anionic surfactants and nonionic surfactants selected were compounded according to the proportions in Table 3, and the foaming performance was evaluated. The screening results are shown in Table 4.

[0033] Table 3 Screening table of compounding ratios of anionic surfactants and nonionic surfactants

[0034]

[0035] Table 4 Foaming performance results of anionic surfactants and nonionic surfactants

[0036]

[0037] It is preferred that the optimal ratio of anionic surfactant to nonionic surfactant is 2.0-2.5, and the nonionic surfactant is relatively optimal with C9-11 polyether-8 and alcohol ether glycoside. The following specific examples will screen the effective ingredients with the optimal ratio of anionic surfactant to nonionic surfactant being 2.25.

[0038] Example 2

[0039] 15-30 parts of organic acid, 1.1 parts of chelated zinc, 1 part of chelated copper, 1.2 parts of chelated manganese, 22.5 parts of anionic surfactant, 10 parts of nonionic surfactant, 0.01 parts of colorant;

[0040] The mass composition of organic acid is: lactic acid:formic acid=5:1 or lactic acid:octanoic acid=5:1 or lactic acid:glycolic acid=5:1;

[0041] The mass composition of the anionic surfactant is: sodium lauryl ether sulfate: sodium α-olefin sulfonate: sodium octyl sulfosuccinate = 1:2.5:1;

[0042] The nonionic surfactant is C9-11 polyether-8, anionic surfactant: nonionic surfactant = 2.25:1

[0043] The coloring agent is bright blue pigment;

[0044] The preparation method comprises the following steps: according to the formula, 15-30 grams of organic acid, 1.1 grams of chelated zinc, 1 gram of chelated copper, 1.2 grams of chelated manganese, 10 grams of surfactant and 0.01 grams of colorant are added with water to dissolve the colorant at room temperature for later use, then the organic acid, chelated copper and chelated manganese are added into a beaker, stirred evenly with a glass rod, and then chelated zinc, chelated manganese, anionic surfactant, nonionic surfactant and colorant are added in sequence while stirring to dissolve. Finally, purified water is added to 100 milliliters, and stirred until there is no solid mixture in the liquid to obtain a compound hoof bath disinfectant.

[0045] Table 5 Prescription information prepared according to the requirements of Example 2

[0046]

[0047] Example 3

[0048] 15-30 parts of organic acid, 1.1 parts of chelated zinc, 1 part of chelated copper, 1.2 parts of chelated manganese, 22.5 parts of anionic surfactant, 10 parts of nonionic surfactant, 0.01 parts of colorant;

[0049] The mass composition of organic acid is: lactic acid:formic acid=5:1 or lactic acid:octanoic acid=5:1 or lactic acid:glycolic acid=5:1;

[0050] The mass composition of the anionic surfactant is: sodium lauryl ether sulfate: sodium α-olefin sulfonate: sodium octyl sulfosuccinate = 1:2.5:1;

[0051] The nonionic surfactant is an alcohol ether glycoside;

[0052] The coloring agent is bright blue pigment;

[0053] The preparation method comprises the following steps: firstly, according to the formula, dissolving the colorant in water at room temperature for later use, then adding the organic acid, chelated copper and chelated manganese into a beaker, stirring evenly with a glass rod, then adding chelated zinc, chelated manganese, anionic surfactant, nonionic surfactant and colorant in sequence while stirring to dissolve. Finally, adding purified water to 100 ml, stirring until there is no solid mixture in the liquid, etc., to obtain a compound hoof bath disinfectant.

[0054] Table 6 Prescription information prepared according to the requirements of Example 3

[0055]

[0056] Example 4

[0057] The effect of the hoof bath disinfectant of the present invention in treating common pathogens of cattle hooves was verified.

[0058] Each prescription was prepared according to Example 2-3, and the standard strains of Escherichia coli, Staphylococcus aureus and Candida albicans were used for quantitative antibacterial experiments. The action time was 5 minutes. The quantitative test results are shown in Table 7.

[0059] According to the results of the quantitative antibacterial test, the organic acid has a good killing effect on Escherichia coli, Staphylococcus aureus and Candida albicans at 25-30. The hemolysis test was carried out on the preferred prescription to further evaluate the irritation of the product to the skin. The test results are shown in Tables 7 and 8.

[0060] Table 7 Quantitative bactericidal test results of prescription 1-24 obtained in Example 2-3 (unit: log CFU·mL -1 )

[0061]

[0062] Note: The disinfection technical specifications require that the killing logarithm value of Staphylococcus aureus and Escherichia coli be ≥5; the killing logarithm value of Candida albicans be ≥4.

[0063] Hemolysis test: To separate red blood cells, add 50 mL of sodium citrate buffer (17.03 g trisodium citrate + 8.45 g citric acid, diluted to 1 L with sterile deionized water) to 450 mL of fresh calf blood and mix. After centrifugation, discard the supernatant to obtain red blood cells (RBC). Then rinse and centrifuge several times with phosphate buffered saline (PBS) (3.15 g disodium hydrogen phosphate + 0.762 g potassium dihydrogen phosphate + 7.21 g sodium chloride + 1.8 g glucose, diluted to 1 L with sterile deionized water) to remove white blood cells and plasma. The final red blood cell stock solution is stored in a container for subsequent testing.

[0064] In addition, a standard surfactant solution was prepared, which was a PBS solution containing 1000 ppm of sodium dodecyl sulfate; the standard solution was diluted with PBS to 0 ppm, 20 ppm, 30 ppm and 40 ppm, respectively. At the same time, a PBS solution containing 1000 ppm of the test product was prepared, and also diluted with PBS to 0 ppm, 25 ppm, 50 ppm and 100 ppm, respectively.

[0065] Determination of H50 value of standard surfactant solution: 9.75 mL of 20 ppm, 30 ppm, and 40 ppm standard surfactant solution were mixed with 0.25 mL of adjusted RBC stock solution, and the mixture was then shaken for 10 min and centrifuged at 5000 rpm for 3 min to precipitate intact RBC. Use 0 ppm standard surfactant solution as a blank sample and measure the absorbance at 560 nm. In addition, 0.25 mL of adjusted RBC stock solution was mixed with 9.75 mL of deionized water, shaken for 10 min, and the absorbance at 560 nm was measured, which is equal to the 100% hemolysis value (H100). The concentration of H50 is determined by plotting a curve of standard surfactant concentration versus absorbance at 560 nm (the H50 concentration is the concentration when the absorbance is equal to half of the H100 absorbance).

[0066] Determination of H50 value of the test product: 9.75 mL of 25 ppm, 50 ppm, and 100 ppm test product solution were mixed with 0.25 mL of the adjusted RBC stock solution, and the mixture was then shaken for 10 min and centrifuged at 5000 rpm for 3 min to precipitate intact RBC. Take 0 ppm test product solution as a blank sample and measure the absorbance at 560 nm. Determine the H50 concentration by plotting the test product solution concentration versus the absorbance at 560 nm (the H50 concentration is the concentration when the absorbance is equal to half of the H100 absorbance).

[0067] Determination of DI value: Mix 0.25mL of the adjusted RBC stock solution with 1000ppm standard surfactant solution, shake for 10min, and centrifuge at 5000rpm for 3min to precipitate intact RBCs. All RBCs should be dissolved. Use 0ppm standard surfactant solution as a blank sample and measure the absorbance at 575nm and 540nm. The ratio of ABS575 / ABS540 is equal to R2. R2 is approximately equal to 0.8. Mix 0.25mL of the adjusted RBC stock solution with 1000ppm test product solution, shake for 10min, and centrifuge at 5000rpm for 3min to precipitate intact RBCs. All RBCs should be dissolved. If the RBCs are not completely dissolved, repeat the treatment with a higher concentration of test product solution. Use 0ppm test product solution as a blank sample to measure the absorbance at 575nm and 540nm. The ratio of ABS575 / ABS540 is equal to Ri.

[0068] Where, DI (%) = 100 (1.05-Ri) / (1.05-R2)

[0069] L / D=H50 / DI

[0070] The L / D value is the main value that usually determines irritation. If L / D ≥ 100, the product is considered non-irritating; if 10 ≤ L / D < 100, the product is considered slightly irritating; if 1 ≤ L / D < 10, the product is considered moderately irritating; if 0.1 ≤ L / D < 1, the product is considered irritating; and if L / D < 0.1, the product is considered severely irritating.

[0071] Table 8 Preferred prescription hemolytic test results

[0072]

[0073] Example 5

[0074] The effect verification of the safety experiment of the hoof bath disinfectant of the present invention.

[0075] In accordance with the Technical Specifications for Disinfection, the prescriptions with L / D values ​​greater than 90 in Table 8 were subjected to guinea pig skin irritation test and allergy test, and the evaluation indicators are shown in Tables 9-10.

[0076] Table 9 Scoring criteria for skin irritation reactions and skin irritation intensity classification

[0077]

[0078] Note: Skin irritation index = total score of erythema and edema for each animal / (number of tested animals*total number of days)

[0079] Table 10 Guinea pig active skin allergy test

[0080]

[0081] Note: Sensitization incidence (%) = number of animals with skin reaction (score ≥ 1) / number of test animals in the group * 100

[0082] The results of the guinea pig skin irritation test and allergy test are shown in Table 11.

[0083] Table 11 Results of skin irritation and allergy test on guinea pigs with the preferred prescription

[0084]

[0085] The above results show that in the skin irritation experiment, the guinea pig back skin did not show obvious irritation symptoms such as bleeding, edema, and scars, indicating that the selected prescription has low irritation to the guinea pig skin; the results of the skin irritation reaction score show that the skin irritation index of the selected prescription irritation experiment is between 0-0.5, indicating that the irritation intensity level of the present invention is non-irritating. Therefore, overall, it shows a relatively mild property on the guinea pig skin and meets the standard for safe use.

[0086] Example 6

[0087] The effect verification of the stability experiment of the hoof bath disinfectant of the present invention.

[0088] The prescription obtained in Example 2-3 was diluted with water at a ratio of 1:49 and placed at 54°C for 14 days to examine the changes in properties and the content of the active ingredient (lactic acid) on the 0th day and the 14th day.

[0089] Table 12 Results of stability study of preferred prescription at 54℃ for 14 days

[0090]

[0091] The results are shown in Table 12. During the inspection period, the color of the disinfectant did not change significantly, and there was no turbidity or precipitation in the wide-mouth bottle, showing a uniform texture; the degradation rate of the active ingredient (lactic acid) was less than 5%, indicating that the present invention exhibits good stability under high temperature conditions.

[0092] Example 7

[0093] Verification of the effect of the hoof bath disinfectant of the present invention on the antibacterial experiment of clinical isolated disease materials

[0094] Hoof disease samples were randomly collected from multiple ranches in Ningxia for identification and culture, and most of the isolated bacteria were Escherichia coli, Staphylococcus aureus and Candida albicans. The results of cattle hoof disease samples (“√” means that this species is contained) are shown in Table 13.

[0095] Table 13 Identification of bacterial species in clinical samples

[0096]

[0097] The detection rate of Escherichia coli in all samples reached 100%. As a common Gram-negative pathogen, Escherichia coli is part of the intestinal flora; the microscopic examination results confirmed the presence of Escherichia coli, indicating that it may be involved in the occurrence of these diseases. In addition, the detection rate of Staphylococcus aureus was 76.47%; this Gram-positive bacterium is commonly found on the skin surface, and its high detection rate suggests that the occurrence of cattle skin diseases may be related to Staphylococcus aureus infection. In contrast, the detection rate of Candida albicans was lower, at 23.52%; this fungus usually grows in a humid and warm environment, and its presence may be related to the humidity and environmental conditions of the hoof. Although the detection rate is low, it also suggests the possibility of fungal infection in the hoof of cattle.

[0098] A quantitative antibacterial test was carried out using mixed bacteria isolated from clinical diseased materials as the test bacteria, and 0%, 10% and 20% calf serum (BSA) were used for organic interference. Compared with the sample containing 0% organic matter, the bactericidal effect is basically the same. This shows that the present invention has a strong anti-interference ability, and can fully exert its own bactericidal effect even in the presence of organic interference. The present invention successfully inhibits the growth and reproduction of Escherichia coli, Staphylococcus aureus and Candida albicans under the interference of different contents of organic matter. This situation shows that the present invention can adapt to different environments and show its ability to adapt to multiple environments. This means that even in the cattle farm environment, in the presence of various organic interferences, the present invention can still effectively remove pathogenic bacteria on the hoof surface without being affected, reduce the incidence of cattle hoof disease, and ensure the health of cattle hooves. The specific quantitative antibacterial test results are shown in Table 14.

[0099] Table 14 Results of quantitative antibacterial test of clinical isolated mixed bacteria

[0100]

[0101] Example 8

[0102] Comparison of in vitro antibacterial effects of the hoof bath disinfectant of the present invention and commercial samples

[0103] The formula selected in Examples 2-3 of the present invention was diluted 50 times with three hoof bath products currently on sale for quantitative antibacterial test comparison. The hoof bath products on the market were named commercial samples 1-3 respectively.

[0104] Commercial sample 1 is a benzalkonium bromide solution (5%), which contains, in addition to benzalkonium bromide, other active ingredients such as thymol, chelated copper, and chelated zinc.

[0105] Commercial sample 2 is zedoary extract (3%), the main components of which include zedoary extract, chelated copper, and chelated zinc.

[0106] Commercial sample 3 is also a benzalkonium bromide solution (5%), and also contains a stabilizer, a transdermal agent, and a wound healing promoting factor.

[0107] Table 15-1 Quantitative antibacterial test results of the preferred formulation of the present invention and commercial samples on Escherichia coli (unit: logCFU·mL -1 )

[0108]

[0109] Table 15-2 Quantitative antibacterial test results of the preferred formulation of the present invention and commercial samples on Staphylococcus aureus (unit: log CFU·mL -1 )

[0110]

[0111] Table 15-3 Quantitative antibacterial test results of the preferred formulation of the present invention and commercial samples on Candida albicans (unit: logCFU·mL -1 )

[0112]

[0113] Table 15-4 Quantitative antibacterial test results of the preferred formulation of the present invention and commercial samples on Streptococcus (unit: log CFU·mL -1 )

[0114]

[0115] Example 9

[0116] Comparison of the effects of the present invention and commercial samples in clinical studies

[0117] Clinical trials were conducted in three small ranches in Ningxia. Hoof baths were used to bathe the hooves of cows after milking according to the technical parameters and usage of the hoof bath. Hoof baths are required to be performed three times a week during the hoof bathing health care process, specifically on Monday, Wednesday, and Friday, for four consecutive weeks. Each time, the medicated bath can provide a maximum of 300-400 cows for hoof bathing. After the number of cows bathing exceeds 300-400, the hoof bathing pool must be cleaned, the hoof bathing liquid must be replaced, and the pool for storing clean water must be replaced at the same time. Before the first hoof bath, 9 cows (gait score of 2 points or more) were randomly selected, the cow number was recorded, and the gap between the 3rd and 4th toes was wiped with a sterile cotton swab. After the first hoof bath for 5 minutes, the gap between the 3rd and 4th toes was wiped with a sterile cotton swab. The clinical effectiveness of the product of the present invention and the commercial sample was judged by comparing the changes in the number of colonies before and after the hoof bath.

[0118] The effectiveness of the present invention in clinical applications was verified by comprehensively investigating and analyzing the bacterial infection of cattle hooves. The samples before and after hoof bathing were tested for the number of colonies on the hoof. The results are shown in Table 16. The number of colonies before hoof bathing was generally high, and the number of colonies could reach up to 8 orders of magnitude. The number of colonies after hoof bathing was significantly reduced to a lower level. There are various factors affecting the clinic. After using the product of the present invention diluted 50 times for cattle hoof bathing, the number of bacteria on the cattle hoof can be reduced by at least nearly 5 orders of magnitude. Although the amount of data is small, since the test was conducted by randomly sampling cattle hooves, the effectiveness of the product of the present invention in clinical applications can still be clearly demonstrated. This shows that the hoof bath disinfectant can effectively remove bacteria on the surface of cattle hooves during use, thereby reducing the risk of infection with cattle hoof diseases.

[0119] Table 16 The number of bacterial colonies on the hooves of the present invention and commercial samples before and after use (in log CFU mL -1 )

[0120]

[0121] The antibacterial logarithmic reduction value of prescription 6 is 5.74±0.43, the antibacterial logarithmic reduction value of prescription 10 is 6.02±0.34, the antibacterial logarithmic reduction value of prescription 22 is 6.24±0.52, and the antibacterial logarithmic reduction value of commercial sample 1 is 5.78±0.47. This shows that the antibacterial effect of the product of the present invention is comparable to that of the product on sale.

[0122] Dairy cow gait score (limb and hoof disease cure rate) - score once before and after the test.

[0123] The scoring rules for visual observation of dairy cow gait are as follows:

[0124] 1 point: The back is straight when standing and walking, and all feet rise and fall in unison;

[0125] 2 points: Slight lameness, with a straight back when standing and an arched back when walking, and a slightly abnormal gait;

[0126] 3 points: moderate lameness, arched back when standing and walking, short steps, and the rise and fall of the hoof concentrated on one hoof;

[0127] 4 points: Clinical symptoms, arched back when standing and walking, obvious preference for one hoof;

[0128] 5 points: severe hoof disease, arched back, refusal to bear weight on one hoof, difficulty in getting up and lying down;

[0129] Table 17 Gait scores before and after hoof bathing at three farms (hoof bathing for 4 weeks)

[0130]

[0131] After 4 weeks of hoof bathing, the health of the hooves in the three ranches was improved. The efficacy of the product of the present invention was equivalent to that of the commercial product, and no irritation or allergic reaction occurred during the hoof bathing, and the clinical safety was good.

[0132] Example 10

[0133] Test of the wetting / adsorption capacity of the foot bath disinfectant of the present invention

[0134] 1 ml of each of the hoof bath solutions of recipes 6, 10, 18, and 22 in Examples 2-3 was taken into a 50 ml beaker, and 49 ml of standard hard water was added and mixed. A 10 ml centrifuge tube was vertically immersed in each of the four dilutions to the 5 ml mark, and then taken out vertically and observed for 5 min, and the height (mm) of the liquid on the outer wall of the centrifuge tube sliding down from the 5 ml mark was measured.

[0135] Table 18 Drop test

[0136] Prescription 6 Prescription 10 Prescription 18 Prescription 22 Time / min 5 5 5 5 Drop height / mm 0 0 0 0

[0137] This embodiment shows that the present invention has good wetting / adsorption ability, good adhesion and permeability.

[0138] Those skilled in the art will readily appreciate other embodiments of the invention after considering the specification and practicing the invention disclosed herein. The present invention is intended to cover any variations, uses or adaptations of the present invention that follow the general principles of the present invention and include common knowledge or customary techniques in the art not disclosed herein. The description and examples are to be regarded as exemplary only, and the true scope of the invention is indicated by the claims.

[0139] It should be understood that the present invention is not limited to what has been described above, and various modifications and changes can be made without departing from its scope. The above-described embodiments of the present invention do not constitute a limitation on the protection scope of the present invention.

Claims

1. A dairy cow hoof bath disinfectant compounded with organic acid and chelated copper, characterized in that: The invention comprises the following components in parts by weight: 1-30 parts of organic acid, 1-5 parts of chelated zinc, 1-5 parts of chelated copper, 1-5 parts of chelated manganese, 10-40 parts of surfactant and 0.01-1 part of colorant.

2. The organic acid and chelated copper compound cow hoof bath disinfectant according to claim 1, characterized in that: The invention comprises the following components in parts by mass: 25 parts of organic acid, 1.1 parts of chelated zinc, 1 part of chelated copper, 1.2 parts of chelated manganese, 22.5 parts of anionic surfactant, 10 parts of nonionic surfactant and 0.01 part of colorant.

3. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The organic acid is any one or more of caprylic acid, formic acid, L-lactic acid, capric acid, glycolic acid, L-malic acid and mandelic acid.

4. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The chelated zinc is any one or more of zinc oxalate, zinc lactate, zinc citrate, and glycine chelated zinc.

5. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The chelated copper is any one or more of citric acid chelated copper, copper citrate, and copper glycinate.

6. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The chelated manganese is any one or more of chelated manganese citrate, manganese citrate, manganese glycinate and manganese edetate.

7. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The anionic surfactant is any one or more of sodium α-olefin sulfonate, sodium dioctyl sulfosuccinate, sodium lauryl ether sulfate, sodium dodecylbenzene sulfonate and sodium lauryl sulfate.

8. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The nonionic surfactant is any one or more of C9-11 polyether-8, alcohol ether glycoside and coconut oil fatty acid diethanolamide.

9. The organic acid and chelated copper compounded dairy cow hoof bath disinfectant according to any one of claims 1 or 2, characterized in that: The colorant is any one or more of brilliant blue, fruit green and lemon yellow.

10. A method for preparing a cow hoof bath disinfectant compounded with an organic acid and chelated copper, based on the cow hoof bath disinfectant compounded with an organic acid and chelated copper according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: firstly, dissolving a colorant in water at room temperature for later use, then adding an organic acid and chelated copper into a container, stirring evenly, then sequentially adding chelated zinc, chelated manganese, anionic surfactant, nonionic surfactant, and colorant while stirring to dissolve. Finally, adding purified water, stirring until the liquid does not contain solid mixture, foam, etc., to obtain a compound hoof bath disinfectant.

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