New application of traditional Chinese medicine composition

Abstract

The invention provides a new application of a traditional Chinese medicine composition, the traditional Chinese medicine composition is composed of glossy privet fruit and eclipta, and research shows that the traditional Chinese medicine composition has neuroprotection and anti-depression effects. In in-vivo experiments, the traditional Chinese medicine composition can effectively relieve stress response of mice after treatment, and the stress response is specifically shown as relieving despair feeling of the mice, improving movement behaviors of the mice, increasing cognitive indexes of the mice, increasing the level of serotonin of monoamine neurotransmitters in serum, reducing the concentration of glucocorticoid in the serum and increasing expression of glucocorticoid receptors in brain tissue.

Description

Technical Field

[0001] The present invention relates to the field of medical application technology, in particular to a new use of a traditional Chinese medicine composition. Background Art

[0002] In modern society, excessive stress and mental pressure have quietly become important factors that threaten people's quality of life and mental health. Scientific research shows that stress may not only damage cognitive function in the short term, but also turn into chronic stress, thus becoming a key risk factor for a series of mental illnesses. At present, stress-related diseases occupy an important position in the field of human health and are increasingly attracting the attention of scholars. Severe stress events are usually associated with diseases such as acute stress response and post-traumatic stress disorder. Long-term stress may increase the risk of degenerative diseases and cerebrovascular diseases. Studies have shown that long-term chronic stress is one of the causes of harmful changes in the brain, plays an important role in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and depression, and may accelerate the progression of the disease. At the same time, stress increases the incidence of cardiovascular disease by 15%-50%, and stress-related physiological disorders lead to an increase in cardiovascular disease mortality and all-cause mortality by 31% and 22%, respectively. In summary, the social harm of stress cannot be ignored, and individuals and society should work together to take effective measures to mitigate its impact.

[0003] As a traditional Chinese medicine formula, Erzhi Pills nourish the liver and kidneys, nourish yin and stop bleeding, nourish and calm the mind. They are often used for liver and kidney yin deficiency, dizziness, tinnitus, dry throat and nose, back and knee pain, and heavy menstruation. Modern medical research has found that Ligustrum lucidum can improve the gonadal and hormone levels of animal models of kidney yin deficiency, as well as the structure of the hypothalamus-pituitary-ovarian axis; the water extract of Eclipta prostrata can significantly increase the content of NE, DA, and 5-HT in the hippocampus of rats with Alzheimer's disease model, and regulate the level of neurotransmitters in the brain. Therefore, further in-depth research on Erzhi Recipe is expected to discover more medical uses. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a new use of a traditional Chinese medicine composition.

[0005] The technical solution adopted by the present invention is:

[0006] A use of a traditional Chinese medicine composition in preparing a neuroprotective drug. The traditional Chinese medicine composition consists of Ligustrum lucidum fruit and Eclipta prostrata, wherein the Ligustrum lucidum fruit comprises 5-8 parts by weight and the Eclipta prostrata fruit comprises 2-5 parts by weight.

[0007] Preferably, in the use of the above-mentioned traditional Chinese medicine composition, the neuroprotective drug is an antidepressant drug.

[0008] Preferably, in the use of the above-mentioned traditional Chinese medicine composition, the Fructus Ligustri Lucidi and Eclampsia Root are calculated by weight as 1 part of Fructus Ligustri Lucidi and 1 part of Eclampsia Root.

[0009] Preferably, the use of the above-mentioned Chinese medicine composition is prepared by the following method:

[0010] (1) Soaking Ligustrum lucidum and Eclipta prostrata in ethanol according to the component amounts, heating under reflux for extraction, and filtering to obtain a decoction;

[0011] (2) After mixing the decoctions, the extract is concentrated using a rotary evaporator and freeze-dried to obtain the product.

[0012] The beneficial effects of the present invention are:

[0013] The Chinese medicine composition of the present invention is composed of Ligustrum lucidum and Eclipta prostrata, and studies have shown that it has neuroprotective and antidepressant effects. In in vivo experiments, the Chinese medicine composition can effectively alleviate the stress response of mice after treatment, specifically by reducing the sense of despair of mice, improving the motor behavior of mice, improving the cognitive index of mice, increasing the level of monoamine neurotransmitter serotonin in serum, reducing the concentration of glucocorticoids in serum, and increasing the expression of glucocorticoid receptors in brain tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a 1% sucrose preference experiment. The sugar preference level (compared with the normal control group, # P<0.05, ## P<0.01, ### P<0.001; compared with the model control group, * P<0.05, ** P<0.01, *** P<0.001).

[0015] Figure 2 For the tail suspension test, the immobility time was accumulated.

[0016] Figure 3 For the forced swim test, the immobility time was accumulated.

[0017] Figure 4 This is the open field test and the activity time of mice.

[0018] Figure 5 This is the open field test, and the activity distance of mice.

[0019] Figure 6 The speed at which mice move in the open field test.

[0020] Figure 7 For the novel object recognition experiment, the number of times the mouse explored the novel object.

[0021] Figure 8 For the novel object recognition experiment, the mouse cognitive index.

[0022] Fig. 9 is the concentration of serotonin in serum.

[0023] Fig.10 is the concentration of corticosterone in serum.

[0024] Fig.11 Figure 2 shows the expression of glucocorticoid receptor in the cerebral cortex of different groups. DETAILED DESCRIPTION

[0025] In order to enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0026] Example 1

[0027] Preparation of Chinese medicine composition

[0028] The preparation method is as follows: 500g of Ligustrum lucidum and 500g of Eclipta prostrata are soaked in 5L 70% ethanol for 0.5h, heated under reflux for extraction for 2h, and filtered to obtain a decoction; 5L 70% ethanol is added again, heated under reflux for extraction for 2h, and the medicinal liquid is filtered out. The two extracts are mixed, the extracts are concentrated using a rotary evaporator, and freeze-dried to obtain the product.

[0029] Example 2

[0030] The preparation method of the Chinese medicine composition is the same as that of Example 1, wherein 600 g of Fructus Ligustri Lucidi and 400 g of Eclipta prostrata are used.

[0031] Example 3

[0032] The preparation method of the Chinese medicine composition is the same as that of Example 1, wherein 800 g of Ligustrum lucidum and 200 g of Eclipta prostrata are used.

[0033] Example 4

[0034] Pharmacological Experimental Study on the Chinese Medicine Composition in Example 1 in Mice with Chronic Mild Unpredictable Stress Depression

[0035] Male SPF C57 / δJ mice, 8 weeks old, were provided with free access to food and water, and were kept in an environment with 12 hours of lighting per day and a temperature of 25±2℃.

[0036] The animals were divided into 5 groups: normal control group, model control group, fluoxetine (Shanghai Shangyao Zhongxi Pharmaceutical Co., Ltd., National Medicine Standard H19980114) positive control group (3.0 mg / kg), Chinese medicine combination low-dose group (3.6 g / kg), Chinese medicine combination high-dose group (7.2 g / kg). A chronic mild unpredictable stress model was established, and two different stimuli were given every day and could not be repeated for two consecutive days for a total of 7 weeks. At the same time as the modeling, therapeutic drugs were given by gavage every day (except the model control group). Behavioral testing began in the 6th week. After the behavioral testing, blood, liver, brain and other tissues were taken for subsequent experiments.

[0037] The 1% sucrose preference test, tail suspension test, and forced swimming test were used to evaluate the emotional state of mice. The open field test was used to evaluate the motor behavior of mice. The novel object recognition test was used to evaluate the cognitive level of mice. The concentrations of serotonin (ELISA MM-0443M1) and corticosterone (ELISA MM-0061M1) in serum were detected by Elisa method. The expression level of glucocorticoid receptor protein in the cortex of brain tissue was detected by Western Blot method.

[0038] The results are as follows Figure 1-11 Compared with the normal control group, # P<0.05, ## P<0.01, ### P<0.001; compared with the model control group, * P<0.05, ** P<0.01, *** P < 0.001. Compared with the normal control group, the model control group had a significantly lower preference for sucrose, a significantly increased immobility time in the tail suspension test and the forced swimming test, a significantly reduced activity distance and activity time in the open field test, a significantly reduced number of explorations of new objects, and a significantly reduced serum serotonin concentration, and mice showed a stress response. The above phenomena were alleviated by giving different concentrations of the Chinese medicine composition described in Example 1 for treatment. Compared with the model control group, the Chinese medicine composition of the present invention can significantly increase the preference for sucrose in mice; it can significantly reduce the immobility time in the tail suspension test and the forced swimming test, it can improve the movement of mice in the open field test, and it can improve the cognitive index of mice. In addition, a high dose of the Chinese medicine composition can significantly increase the concentration of serotonin in serum. Compared with the normal control group, the serum cortical corticosterone concentration of the model control group was significantly increased, and the expression of glucocorticoid receptors in the cerebral cortex was significantly reduced. After the intervention of the Chinese medicine composition, the decrease in corticosterone concentration and the decrease in glucocorticoid receptors were inhibited. In summary, the Chinese medicine composition of the present invention can reduce the nervous system response of stressed mice, relieve emotional disorders of stressed mice, improve the motor ability of mice, upregulate the level of monoamine neurotransmitter serotonin, and increase the expression of glucocorticoid receptors.

[0039] The above experiment was repeated using the Chinese medicine composition described in Examples 2 and 3, which also had neuroprotective and antidepressant effects.

[0040] The above-described embodiments are merely descriptions of preferred implementations of the present invention and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary engineers and technicians in the field should all fall within the protection scope of the present invention.

Claims

1. Use of a Chinese medicine composition in the preparation of a neuroprotective drug, characterized in that: The traditional Chinese medicine composition consists of Ligustrum lucidum fruit and Eclipta prostrata, wherein the Ligustrum lucidum fruit comprises 5-8 parts by weight and the Eclipta prostrata fruit comprises 2-5 parts by weight.

2. The use according to claim 1, characterized in that: The neuroprotective drug is an antidepressant drug.

3. The use according to claim 1, characterized in that: The glossy privet fruit and Eclipta prostrata are calculated by weight as follows: 1 part of glossy privet fruit and 1 part of Eclipta prostrata.

4. The use according to any one of claims 1 to 3, characterized in that: The Chinese medicine composition is prepared by the following method: (1) Soaking Ligustrum lucidum and Eclipta prostrata in ethanol according to the component amounts, heating under reflux for extraction, and filtering to obtain a decoction; (2) After mixing the decoctions, the extract is concentrated using a rotary evaporator and freeze-dried to obtain the product.

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