Traditional Chinese medicine composition for preventing female ovulation dysfunction and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine composition for preventing female ovulation dysfunction and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The feed is prepared from the following components in parts by weight: 10 to 20 parts of radix polygoni multiflori, 2 to 4 parts of radix rehmanniae praeparata, 2 to 4 parts of poria cocos, 5 to 12 parts of fermented pinellia tuber, 1 to 3 parts of exocarpium citri rubrum, 10 to 25 parts of beans, 2 to 8 parts of vitamin E, 4 to 9 parts of folic acid, 10 to 30 parts of green vegetables, 5 to 8 parts of additives, 8 to 15 parts of fructus corni, 5 to 10 parts of cortex eucommiae, 2 to 10 parts of meridian guiding medicine, 5 to 20 parts of radix angelicae sinensis, 1 to 9 parts of clomiphen, 1 to 3 parts of herba epimedii and 10 to 20 parts of brown sugar. The traditional Chinese medicine composition for preventing female ovulation dysfunction improves ovulation of traditional Chinese medicines and treats deficiency of corpus luteum function, improves the prevention effect of the traditional Chinese medicines, and obviously improves the problems of gastrointestinal discomfort, allergy and the like caused by long-term use or improper use of the traditional Chinese medicines.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine compositions, in particular to a Chinese medicine composition for preventing female ovulation dysfunction and a preparation method thereof. Background Art

[0002] Female ovulatory dysfunction is a gynecological disease, specifically referring to persistent anovulation or irregular ovulation due to a variety of reasons. This disease is common in premature ovarian failure, polycystic ovary syndrome, unruptured luteinized follicle syndrome, hyperprolactinemia, amenorrhea, congenital gonadal dysgenesis and other diseases, and may cause a series of clinical manifestations, such as irregular menstruation, infertility, obesity, hirsutism, etc. The prevention of female ovulatory dysfunction usually does not rely on specific drugs, because the causes of ovulatory disorders are complex and diverse, including abnormalities in multiple links such as the hypothalamus, pituitary gland, and ovaries. However, in some specific cases, drugs can be used to assist in the treatment or regulate endocrine function, thereby preventing or improving ovulatory disorders. In the treatment of ovulatory disorders, Chinese medicine mainly achieves therapeutic effects by regulating the body's qi and blood, the functions of internal organs and meridians, and the balance of yin and yang. Traditional Chinese medicine believes that ovulation disorders are related to multiple factors such as kidney deficiency, liver depression, phlegm and dampness, spleen deficiency, etc. Therefore, the selection of Chinese medicine drugs will also be based on these causes. Some commonly used Chinese medicine drugs such as Zishen Yutai Pills, Bazhen Pills, Xiaoyao Pills, etc., have the effects of warming the kidney and replenishing qi, nourishing blood and regulating menstruation, soothing the liver and relieving depression in traditional Chinese medicine theory. They are suitable for patients with different types of ovulation disorders. These Chinese medicines can promote the normal development and ovulation of follicles by improving the patient's physical condition and endocrine environment. However, the existing drugs for the treatment of female ovulation dysfunction still have many shortcomings. Chinese medicine treatment of ovulation disorders can usually only be used as an auxiliary treatment method. For patients with severe ovulation disorders, relying solely on Chinese medicine cannot achieve the ideal therapeutic effect, and long-term use or improper use will cause gastrointestinal discomfort, allergies, etc. Summary of the invention

[0003] The purpose of the present invention is to provide a Chinese medicine composition for preventing female ovulatory dysfunction and a preparation method thereof, so as to solve the problems that the existing Chinese medicine for treating female ovulatory dysfunction mentioned in the above background technology still has many shortcomings. For patients with severe ovulatory dysfunction, relying solely on Chinese medicine cannot achieve the ideal therapeutic effect, and long-term use or improper use may cause gastrointestinal discomfort, allergies and the like.

[0004] To achieve the above purpose, the present invention provides the following technical solution: a traditional Chinese medicine composition for preventing female ovulatory dysfunction, wherein the components include, by weight: 10-20 parts of Polygonum multiflorum, 2-4 parts of Rehmannia root, 2-4 parts of Poria cocos, 5-12 parts of Pinellia tuber, 1-3 parts of Citrus aurantium, 10-25 parts of beans, 2-8 parts of vitamin E, 4-9 parts of folic acid, 10-30 parts of green leafy vegetables, 5-8 parts of additives, 8-15 parts of Cornus officinalis, 5-10 parts of Eucommia ulmoides, 2-10 parts of meridian-guiding drugs, 5-20 parts of Angelica sinensis, 1-9 parts of Clomiphene, 1-3 parts of Epimedium, and 10-20 parts of brown sugar.

[0005] Preferably, the beans are a mixture of soybeans and black beans, and the green leafy vegetables are one or a mixture of spinach, celery and lettuce.

[0006] Preferably, the additive is a mixture of astragalus and wolfberry.

[0007] Preferably, the meridian-guiding drug is cinnamon twig.

[0008] Preferably, the ratio of Rehmannia glutinosa to Poria cocos is 1:1-2.

[0009] A method for preparing a Chinese medicine composition for preventing female ovulation dysfunction comprises the following steps:

[0010] S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, meridian-guiding herbs, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, meridian-guiding herbs, and Angelica sinensis to remove impurities and sediment on the surface;

[0011] S2, soaking the washed Polygonum multiflorum, beans, meridian-inducing herbs and Angelica sinensis in clean water, and then pouring them into a decoction pot for decoction to obtain a No. 1 medicinal solution;

[0012] S3. Weigh tangerine peel, green leafy vegetables, Rehmannia root, Poria cocos, Pinellia tuber, Epimedium and brown sugar, crush the Rehmannia root, Poria cocos, Pinellia tuber and Epimedium, pour the crushed ingredients into liquid medicine No. 1, then add tangerine peel, green leafy vegetables, Epimedium and brown sugar for decoction to obtain liquid medicine No. 2.

[0013] S4. Pour the No. 1 and No. 2 medicinal solutions into a container, filter out the medicinal residues, repeat the decoction 1-2 times, combine the medicinal solutions from each decoction, and obtain the decoction filtrate.

[0014] S5, concentrating the decoction filtrate to obtain No. 1 extract;

[0015] S6, mixing the additive treated in S1, Cornus officinalis, and Eucommia ulmoides, and decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract having a relative density of 1.4-1.6 at 70-85° C.;

[0016] S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 30-40° C., and cooling to room temperature to obtain a mixed paste;

[0017] S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

[0018] Preferably, the step of soaking the washed Polygonum multiflorum, beans, meridian-guiding herbs and Angelica sinensis in clean water, and then pouring them into a decoction pot for decoction to obtain No. 1 medicinal solution specifically comprises the following steps:

[0019] S21, soaking the washed Polygonum multiflorum, beans, meridian-guiding herbs and Angelica sinensis in clean water for 1-3 hours;

[0020] S22. Pour the soaked medicinal materials together with the soaking water into the decoction pot, and add appropriate amount of clean water, the amount of water added should exceed the surface of the medicinal materials by 3-6cm.

[0021] Preferably, the step of weighing tangerine peel, green leafy vegetables, Rehmannia root, Poria cocos, Pinellia tuber, Epimedium and brown sugar in S3, crushing the Rehmannia root, Poria cocos, Pinellia tuber and Epimedium, pouring the crushed ingredients into the No. 1 medicinal solution, and then adding tangerine peel, green leafy vegetables, Epimedium and brown sugar for decoction to obtain the No. 2 medicinal solution specifically comprises the following steps:

[0022] S31, weighing tangerine peel, green leafy vegetables, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crushing Rehmannia root, Poria, Pinellia tuber, Epimedium to 70-140 mesh and pouring into No. 1 medicine solution;

[0023] S32, first pour tangerine peel, green leafy vegetables and epimedium into the liquid medicine, and boil the liquid medicine over high heat;

[0024] S33. Then add brown sugar, turn to low heat and simmer for 30-55 minutes. Meanwhile, stir continuously during the simmering process to obtain medicinal solution No. 2.

[0025] Preferably, the step of concentrating the decoction filtrate in S5 to obtain No. 1 extract comprises the following steps:

[0026] S51, concentrating the decoction filtrate to a liquid having a relative density of 1.1-1.2 at 70-85°C;

[0027] S52, after being allowed to cool to room temperature, alcohol precipitation treatment is performed;

[0028] S53, then let it stand and take out the supernatant to concentrate it to a No. 1 extract with a relative density of 1.2-1.3 at 70-85°C.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] By adding additives, which are a mixture of astragalus and wolfberry, astragalus and wolfberry play a certain role in preventing female ovulation dysfunction, can regulate endocrine, improve ovarian microenvironment, increase pregnancy rate and enhance immune function, and have the effects of nourishing liver and kidney and promoting ovulation. The combination of the two can more comprehensively improve women's reproductive health and prevent the occurrence of ovulation dysfunction;

[0031] By adding the guiding medicine cinnamon twig and clomiphene, the addition of cinnamon twig can achieve the effects of warming the meridians and dispelling cold, regulating menstruation, replenishing blood and activating blood circulation, and promoting endometrial growth after women take it, and prevent female ovulation dysfunction. At the same time, combined with clomiphene as an auxiliary medicine, it can improve ovulation and treat luteal function deficiency, and improve gastrointestinal discomfort, allergies and other conditions that may be caused by long-term or improper use of traditional Chinese medicine. Clomiphene is a commonly used ovulation-inducing drug that can promote follicle development and ovulation. The combined use of clomiphene and traditional Chinese medicine can work synergistically to further increase the ovulation rate. Clomiphene further improves ovarian function by promoting follicle development and ovulation, thereby improving the prevention effect. DETAILED DESCRIPTION

[0032] The invention provides a technical solution: a traditional Chinese medicine composition for preventing female ovulation dysfunction, wherein the components include, by weight: 10-20 parts of Polygonum multiflorum, 2-4 parts of Rehmannia root, 2-4 parts of Poria cocos, 5-12 parts of Pinellia tuber, 1-3 parts of Citrus aurantium, 10-25 parts of beans, 2-8 parts of vitamin E, 4-9 parts of folic acid, 10-30 parts of green leafy vegetables, 5-8 parts of additives, 8-15 parts of Cornus officinalis, 5-10 parts of Eucommia ulmoides, 2-10 parts of meridian-inducing drugs, 5-20 parts of Angelica sinensis, 1-9 parts of Clomiphene, 1-3 parts of Epimedium, and 10-20 parts of brown sugar.

[0033] Among them, the beans are a mixture of soybeans and black beans, and the green leafy vegetables are one or a mixture of spinach, celery, and lettuce. Vitamins are supplemented by green leafy vegetables to prevent and treat female ovulation dysfunction.

[0034] Among them, the additive is a mixture of astragalus and wolfberry. Astragalus and wolfberry play a certain role in preventing female ovulation dysfunction. They can regulate endocrine, improve ovarian microenvironment, increase pregnancy rate and enhance immune function. At the same time, they have the effects of nourishing the liver and kidney and promoting ovulation. The combination of the two can more comprehensively improve women's reproductive health and prevent the occurrence of ovulation dysfunction.

[0035] Among them, the meridian-guiding medicine is cinnamon twig. The addition of cinnamon twig and angelica can achieve the effects of warming the meridians and dispelling cold, regulating menstruation, replenishing blood and activating blood circulation, and promoting endometrial growth after women take it, and prevent female ovulation dysfunction. At the same time, combined with clomiphene as an auxiliary medicine, it improves the ovulation of traditional Chinese medicine and treats the deficiency of corpus luteum function, thereby improving the preventive effect.

[0036] Among them, the ratio of Rehmannia root and Poria cocos is 1:1-2.

[0037] The present invention also provides a method for preparing a Chinese medicine composition for preventing female ovulation dysfunction, comprising the following steps:

[0038] S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, meridian-guiding herbs, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, meridian-guiding herbs, and Angelica sinensis to remove impurities and sediment on the surface;

[0039] S2, soaking the washed Polygonum multiflorum, beans, meridian-inducing herbs and Angelica sinensis in clean water, and then pouring them into a decoction pot for decoction to obtain a No. 1 medicinal solution;

[0040] S3. Weigh tangerine peel, green leafy vegetables, Rehmannia root, Poria cocos, Pinellia tuber, Epimedium and brown sugar, crush the Rehmannia root, Poria cocos, Pinellia tuber and Epimedium, pour the crushed ingredients into liquid medicine No. 1, then add tangerine peel, green leafy vegetables, Epimedium and brown sugar for decoction to obtain liquid medicine No. 2.

[0041] S4. Pour the No. 1 and No. 2 medicinal solutions into a container, filter out the medicinal residues, repeat the decoction 1-2 times, combine the medicinal solutions from each decoction, and obtain the decoction filtrate.

[0042] S5, concentrating the decoction filtrate to obtain No. 1 extract;

[0043] S6, mixing the additive treated in S1, Cornus officinalis, and Eucommia ulmoides, and decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract having a relative density of 1.4-1.6 at 70-85° C.;

[0044] S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 30-40° C., and cooling to room temperature to obtain a mixed paste;

[0045] S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

[0046] Among them, in S2, the washed Polygonum multiflorum, beans, meridian-inducing herbs and Angelica sinensis are soaked in clean water, and then poured into a decoction pot for decoction to obtain a No. 1 medicinal solution, which specifically includes the following steps:

[0047] S21, soaking the washed Polygonum multiflorum, beans, meridian-guiding herbs and Angelica sinensis in clean water for 1-3 hours;

[0048] S22. Pour the soaked medicinal materials together with the soaking water into the decoction pot, and add appropriate amount of clean water, the amount of water added should exceed the surface of the medicinal materials by 3-6cm.

[0049] Among them, the steps of weighing tangerine peel, green leafy vegetables, Rehmannia root, Poria cocos, Pinellia tuber, Epimedium and brown sugar in S3, crushing Rehmannia root, Poria cocos, Pinellia tuber and Epimedium, pouring the crushed ingredients into the No. 1 medicinal solution, and then adding tangerine peel, green leafy vegetables, Epimedium and brown sugar for decoction to obtain the No. 2 medicinal solution specifically include the following steps:

[0050] S31, weighing tangerine peel, green leafy vegetables, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crushing Rehmannia root, Poria, Pinellia tuber, Epimedium to 70-140 mesh and pouring into No. 1 medicine solution;

[0051] S32, first pour tangerine peel, green leafy vegetables and epimedium into the liquid medicine, and boil the liquid medicine over high heat;

[0052] S33. Then add brown sugar, turn to low heat and simmer for 30-55 minutes. Meanwhile, stir continuously during the simmering process to obtain medicinal solution No. 2.

[0053] The step of concentrating the decoction filtrate in S5 to obtain No. 1 extract comprises the following steps:

[0054] S51, concentrating the decoction filtrate to a liquid having a relative density of 1.1-1.2 at 70-85°C;

[0055] S52, after being allowed to cool to room temperature, alcohol precipitation treatment is performed;

[0056] S53, then let it stand and take out the supernatant to concentrate it to a No. 1 extract with a relative density of 1.2-1.3 at 70-85°C.

[0057] Example 1

[0058] This embodiment provides a Chinese medicine composition for preventing female ovulation dysfunction, wherein the components are calculated by weight as follows: 20 parts of Polygonum multiflorum, 4 parts of Rehmannia glutinosa, 4 parts of Poria cocos, 12 parts of Pinellia tuber, 3 parts of Citrus aurantium, 25 parts of beans, 8 parts of vitamin E, 9 parts of folic acid, 30 parts of spinach, 8 parts of additives, 15 parts of Cornus officinalis, 10 parts of Eucommia ulmoides, 10 parts of Cinnamomum cassia, 20 parts of Angelica sinensis, 9 parts of Clomiphene citrate, 3 parts of Epimedium, and 20 parts of brown sugar. As shown in Table 1.

[0059] Table 1

[0060]

[0061] This embodiment also provides a method for preparing a Chinese medicine composition for preventing female ovulation dysfunction, comprising the following steps:

[0062] S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis to remove surface impurities and sediment;

[0063] S2. Soak the washed Polygonum multiflorum, beans, cinnamon twigs and angelica in clean water for 3 hours. Pour the soaked herbs together with the soaking water into the medicine decoction pot, and add an appropriate amount of clean water, the amount of water added should exceed 6 cm above the surface of the herbs.

[0064] S3. Weigh tangerine peel, spinach, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crush Rehmannia root, Poria, Pinellia tuber and Epimedium to 140 mesh and pour into No. 1 medicinal solution, first pour tangerine peel, spinach and Epimedium into the medicinal solution, boil the medicinal solution over high heat, then add brown sugar, turn to low heat and simmer for 55 minutes, and continue stirring during the decocting process to obtain No. 2 medicinal solution.

[0065] S4. Pour the No. 1 and No. 2 medicinal solutions into a container, filter out the medicinal residues, repeat the decoction twice, combine the medicinal solutions from each decoction, and obtain the decoction filtrate.

[0066] S5, concentrating the decoction filtrate to a liquid with a relative density of 1.2 at 85°C, placing it at room temperature and then performing alcohol precipitation, then taking the supernatant and concentrating it to a No. 1 extract with a relative density of 1.3 at 85°C;

[0067] S6, mixing the additive treated in S1, Cornus officinalis and Eucommia ulmoides, decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract with a relative density of 1.6 at 85° C.;

[0068] S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 40° C., and cooling to room temperature to obtain a mixed paste;

[0069] S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

[0070] Example 2

[0071] This embodiment provides a Chinese medicine composition for preventing female ovulation dysfunction, and each component is calculated by weight: Polygonum multiflorum: 10 parts, Rehmannia root: 2 parts, Poria: 2 parts, Pinellia tuber: 5 parts, Tangerine peel: 1 part, beans: 10 parts, vitamin E: 2 parts, folic acid: 4 parts, spinach: 10 parts, additives: 5 parts, Cornus officinalis: 8 parts, Eucommia ulmoides: 5 parts, Cinnamon twig: 2 parts, Angelica sinensis: 5 parts, Clomiphene: 1 part, Epimedium: 1 part, and brown sugar: 10 parts. As shown in Table 2.

[0072] Table 2

[0073]

[0074] This embodiment also provides a method for preparing a Chinese medicine composition for preventing female ovulation dysfunction, comprising the following steps:

[0075] S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis to remove surface impurities and sediment;

[0076] S2. Soak the washed Polygonum multiflorum, beans, cinnamon twigs and angelica in clean water for 1 hour, pour the soaked herbs together with the soaking water into the medicine decoction pot, and add an appropriate amount of clean water, the amount of water added should exceed 3 cm above the surface of the herbs;

[0077] S3. Weigh tangerine peel, spinach, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crush Rehmannia root, Poria, Pinellia tuber and Epimedium to 70 mesh and pour into No. 1 medicinal solution, first pour tangerine peel, spinach and Epimedium into the medicinal solution, boil the medicinal solution over high heat, then add brown sugar, turn to low heat and simmer for 30 minutes, and continue stirring during the decocting process to obtain No. 2 medicinal solution.

[0078] S4. Pour the No. 1 and No. 2 medicinal solutions into a container, filter out the medicinal residues, repeat the decoction once, combine the medicinal solutions from each decoction, and obtain the decoction filtrate.

[0079] S5, concentrating the decoction filtrate to a liquid with a relative density of 1.1 at 70°C, placing it at room temperature and then performing alcohol precipitation, then taking the supernatant and concentrating it to a No. 1 extract with a relative density of 1.2 at 70°C after standing;

[0080] S6, mixing the additive treated in S1, Cornus officinalis, and Eucommia ulmoides, and decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract having a relative density of 1.4 at 70° C.;

[0081] S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 30° C., and cooling to room temperature to obtain a mixed paste;

[0082] S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

[0083] Example 3

[0084] This embodiment provides a Chinese medicine composition for preventing female ovulation dysfunction, and each component is calculated by weight: Polygonum multiflorum: 15 parts, Rehmannia root: 3 parts, Poria: 3 parts, Pinellia tuber: 9 parts, Tangerine peel: 2 parts, beans: 20 parts, vitamin E: 6 parts, folic acid: 6 parts, spinach: 20 parts, additives: 7 parts, Cornus officinalis: 11 parts, Eucommia ulmoides: 8 parts, Cinnamon twig: 7 parts, Angelica sinensis: 12 parts, Clomiphene: 5 parts, Epimedium: 2 parts, and brown sugar: 15 parts. As shown in Table 3.

[0085] Table 3

[0086]

[0087]

[0088] This embodiment also provides a method for preparing a Chinese medicine composition for preventing female ovulation dysfunction, comprising the following steps:

[0089] S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis to remove surface impurities and sediment;

[0090] S2. Soak the washed Polygonum multiflorum, beans, cinnamon twigs and angelica in clean water for 2 hours, pour the soaked herbs together with the soaking water into the medicine decoction pot, and add an appropriate amount of clean water, the amount of water added should exceed the surface of the herbs by 5 cm;

[0091] S3. Weigh tangerine peel, spinach, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crush Rehmannia root, Poria, Pinellia tuber and Epimedium to 110 mesh and pour into No. 1 medicinal solution, first pour tangerine peel, spinach and Epimedium into the medicinal solution, boil the medicinal solution over high heat, then add brown sugar, turn to low heat and simmer for 45 minutes, and continue stirring during the decocting process to obtain No. 2 medicinal solution.

[0092] S4. Pour the No. 1 and No. 2 medicinal solutions into a container, filter out the medicinal residues, repeat the decoction twice, combine the medicinal solutions from each decoction, and obtain the decoction filtrate.

[0093] S5, concentrating the decoction filtrate to a liquid with a relative density of 1.1 at 80°C, placing it at room temperature and then performing alcohol precipitation, then taking the supernatant and concentrating it to a No. 1 extract with a relative density of 1.2 at 80°C after standing;

[0094] S6, mixing the additive treated in S1, Cornus officinalis, and Eucommia ulmoides, and decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract with a relative density of 1.5 at 80° C.;

[0095] S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 35° C., and cooling to room temperature to obtain a mixed paste;

[0096] S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

[0097] Comparative Example

[0098] Different from Example 1, this comparative example provides a Chinese medicine composition for preventing female ovulation dysfunction, and each component is calculated by weight: Polygonum multiflorum: 20 parts, Rehmannia root: 4 parts, Poria: 4 parts, Pinellia tuber: 12 parts, Tangerine peel: 3 parts, beans: 25 parts, vitamin E: 8 parts, folic acid: 9 parts, spinach: 30 parts, additives: 8 parts, Cornus officinalis: 15 parts, Eucommia ulmoides: 10 parts, Cinnamon twig: 10 parts, Angelica sinensis: 20 parts, Epimedium: 3 parts, brown sugar: 20 parts. As shown in Table 4.

[0099] Table 4

[0100]

[0101]

[0102] This comparative example also provides a method for preparing a Chinese medicine composition for preventing female ovulation dysfunction, comprising the following steps:

[0103] S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, cinnamon twig, and Angelica sinensis to remove surface impurities and sediment;

[0104] S2. Soak the washed Polygonum multiflorum, beans, cinnamon twigs and angelica in clean water for 3 hours. Pour the soaked herbs together with the soaking water into the medicine decoction pot, and add an appropriate amount of clean water, the amount of water added should exceed 6 cm above the surface of the herbs.

[0105] S3. Weigh tangerine peel, spinach, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crush Rehmannia root, Poria, Pinellia tuber and Epimedium to 140 mesh and pour into No. 1 medicinal solution, first pour tangerine peel, spinach and Epimedium into the medicinal solution, boil the medicinal solution over high heat, then add brown sugar, turn to low heat and simmer for 55 minutes, and continue stirring during the decocting process to obtain No. 2 medicinal solution.

[0106] S4. Pour the No. 1 and No. 2 medicinal solutions into a container, filter out the medicinal residues, repeat the decoction twice, combine the medicinal solutions from each decoction, and obtain the decoction filtrate.

[0107] S5, concentrating the decoction filtrate to a liquid with a relative density of 1.2 at 85°C, placing it at room temperature and then performing alcohol precipitation, then taking the supernatant and concentrating it to a No. 1 extract with a relative density of 1.3 at 85°C;

[0108] S6, mixing the additive treated in S1, Cornus officinalis and Eucommia ulmoides, decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract with a relative density of 1.6 at 85° C.;

[0109] S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 40° C., and cooling to room temperature to obtain a mixed paste;

[0110] S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

[0111] Experimental example

[0112] Experimental subjects: 160 female volunteers aged between 25 and 35 years old diagnosed with ovulatory dysfunction were selected; the volunteers were randomly divided into three groups, 40 people in each group, and received treatment with the Chinese medicine composition of Example 1, Example 2, Example 3, and the comparative example, respectively.

[0113] Treatment method: Each group of volunteers took the Chinese medicine composition of the corresponding example every day for 3 months. During the treatment, the volunteers' ovulation, hormone level changes and any adverse reactions were recorded.

[0114] Evaluation Metrics:

[0115] Ovulation rate: the proportion of volunteers who successfully ovulate after treatment;

[0116] Hormone levels: including changes in the levels of key hormones such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2) and progesterone (P);

[0117] Ovulation rate: Detected by vaginal B-ultrasound, the monitoring frequency is determined by the average diameter of the follicles. If the follicle diameter is less than 10 mm, it is usually monitored once every three days; if the follicle diameter is between 10 and 15 mm, it is monitored once every two days; if the follicle diameter is greater than 15 mm, it needs to be monitored once a day until the follicle matures and ovulates two days. B-ultrasound monitoring is required continuously. The doctor observes the size, shape, number of follicles and the thickness and shape of the endometrium under B-ultrasound, and records the growth and development of the follicles, as well as whether the follicles rupture and discharge eggs;

[0118] Example 1 group: 85% (34 / 40);

[0119] Example 2 group: 65% (26 / 40);

[0120] Example 3 group: 75% (30 / 40);

[0121] Comparative group: 45% (18 / 40);

[0122] Changes in hormone levels (unit: pg / mL, specific values ​​are determined by experiments):

[0123] Follicle-stimulating hormone (FSH):

[0124] Example 1 group: 7.2 before treatment, 10.5 after treatment;

[0125] Example 2 group: 8.5 before treatment, 10.8 after treatment;

[0126] Example 3 group: 7.8 before treatment, 10.2 after treatment;

[0127] Comparative group: 7.5 before treatment, 8.3 after treatment;

[0128] Luteinizing hormone (LH):

[0129] Example 1 group: 10.1 before treatment, 15.3 after treatment;

[0130] Example 2 group: 12.5 before treatment, 16.1 after treatment;

[0131] Example 3 group: 11.2 before treatment, 14.9 after treatment;

[0132] Comparative group: 10.3 before treatment, 11.8 after treatment;

[0133] Estrogen (E2):

[0134] Example 1 group: 30.5 before treatment, 80.2 after treatment;

[0135] Example 2 group: 29.8 before treatment, 60.5 after treatment;

[0136] Example 3 group: 30.1 before treatment, 70.3 after treatment;

[0137] Comparative group: 32.8 before treatment, 59.8 after treatment;

[0138] Progesterone (P):

[0139] Example 1 group: 0.5 before treatment, 3.2 after treatment;

[0140] Example 2 group: 0.4 before treatment, 2.5 after treatment;

[0141] Example 3 group: 0.5 before treatment, 2.8 after treatment;

[0142] Comparative group: 0.6 before treatment, 1.6 after treatment;

[0143] Incidence of adverse reactions:

[0144] Example 1 group: 0 (0 / 40, no gastrointestinal discomfort);

[0145] Example 2 group: 10% (4 / 40, including 2 cases of gastrointestinal discomfort and 2 cases of mild allergic reactions);

[0146] Example 3 group: 7.5% (3 / 40, mild gastrointestinal discomfort);

[0147] Comparative group: 70% (28 / 40, 25 cases of gastrointestinal discomfort and 3 cases of moderate allergic reactions).

[0148] According to the comparative experimental data of the Chinese medicine composition treatment of Example 1, Example 2, Example 3 and the comparative example group, Example 1-3 groups are more obvious in promoting ovulation of volunteers, and have low adverse reactions. Among them, Example 1 group showed the highest ovulation rate and the most significant improvement in hormone levels in preventing female ovulation dysfunction, and the incidence of adverse reactions was relatively low, while the ovulation and adverse reactions of the volunteers in the comparative example were not good. Combining clomiphene as an adjuvant, the ovulation of Chinese medicine and the treatment of luteal function deficiency were improved, the prevention effect was improved, and the gastrointestinal discomfort, allergies and the like caused by long-term use or improper use of Chinese medicine were significantly improved.

[0149] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicine composition for preventing female ovulation dysfunction, characterized in that: The components include, by weight: Polygonum multiflorum: 10-20 parts, Rehmannia root: 2-4 parts, Poria: 2-4 parts, Pinellia tuber: 5-12 parts, Tangerine peel: 1-3 parts, beans: 10-25 parts, vitamin E: 2-8 parts, folic acid: 4-9 parts, green leafy vegetables: 10-30 parts, additives: 5-8 parts, Cornus officinalis: 8-15 parts, Eucommia ulmoides: 5-10 parts, meridian-guiding drugs: 2-10 parts, Angelica sinensis: 5-20 parts, Clomiphene: 1-9 parts, Epimedium: 1-3 parts, and brown sugar: 10-20 parts.

2. A Chinese medicine composition for preventing female ovulation dysfunction according to claim 1, characterized in that: The beans are a mixture of soybeans and black beans, and the green leafy vegetables are one or a mixture of spinach, celery and lettuce.

3. A Chinese medicine composition for preventing female ovulation dysfunction according to claim 2, characterized in that: The additive is a mixture of astragalus and wolfberry.

4. A Chinese medicine composition for preventing female ovulation dysfunction according to claim 3, characterized in that: The meridian-guiding medicine is cinnamon twig.

5. A Chinese medicine composition for preventing female ovulation dysfunction according to claim 4, characterized in that: The ratio of Rehmannia glutinosa to Poria cocos is 1:1-2.

6. A method for preparing a Chinese medicine composition for preventing female ovulatory dysfunction according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Weighing Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, meridian-guiding herbs, and Angelica sinensis, and washing the weighed Polygonum multiflorum, beans, additives, Cornus officinalis, Eucommia ulmoides, meridian-guiding herbs, and Angelica sinensis to remove impurities and sediment on the surface; S2, soaking the washed Polygonum multiflorum, beans, meridian-inducing herbs and Angelica sinensis in clean water, and then pouring them into a decoction pot for decoction to obtain a No. 1 medicinal solution; S3, weighing tangerine peel, green leafy vegetables, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crushing Rehmannia root, Poria, Pinellia tuber, Epimedium and crushed, pouring into No. 1 medicine solution, then adding tangerine peel, green leafy vegetables, Epimedium and brown sugar for decoction to obtain No. 2 medicine solution; S4. Filter out the medicinal residue, pour the No. 1 and No. 2 medicinal solutions into a container, repeatedly boil the medicinal residue for 1-2 times, combine the medicinal solutions boiled each time, and obtain a decoction. S5, concentrating the decoction to obtain No. 1 extract; S6, mixing the additive treated in S1, Cornus officinalis, and Eucommia ulmoides, and decocting with water, filtering the decoction, and concentrating the filtrate to a No. 2 extract having a relative density of 1.4-1.6 at 70-85° C.; S7, weighing clomiphene and vitamin E, heating and mixing clomiphene, vitamin E, extract A and extract B to 30-40° C., and cooling to room temperature to obtain a mixed paste; S8. Weigh folic acid, mix the mixed paste with the folic acid, and let it stand to obtain a Chinese medicine composition for preventing female ovulation dysfunction.

7. The method for preparing a Chinese medicine composition for preventing female ovulation dysfunction according to claim 6, characterized in that: The step S2 of soaking the washed Polygonum multiflorum, beans, meridian-inducing herbs and Angelica sinensis in clean water, and then pouring them into a decoction pot for decoction to obtain a No. 1 medicinal solution specifically comprises the following steps: S21, soaking the washed Polygonum multiflorum, beans, meridian-guiding herbs and Angelica sinensis in clean water for 1-3 hours; S22. Pour the soaked medicinal materials together with the soaking water into the decoction pot, and add appropriate amount of clean water, the amount of water added should exceed the surface of the medicinal materials by 3-6cm.

8. The method for preparing a Chinese medicine composition for preventing female ovulation dysfunction according to claim 7, characterized in that: The step S3 of weighing tangerine peel, green leafy vegetables, Radix Rehmanniae, Poria, Radix Pinelliae, Epimedium and brown sugar, crushing the Radix Rehmanniae, Poria, Radix Pinelliae and Epimedium, pouring the crushed herbs into the No. 1 medicinal solution, and then adding tangerine peel, green leafy vegetables, epimedium and brown sugar for decoction to obtain the No. 2 medicinal solution specifically comprises the following steps: S31, weighing tangerine peel, green leafy vegetables, Rehmannia root, Poria, Pinellia tuber, Epimedium and brown sugar, crushing Rehmannia root, Poria, Pinellia tuber, Epimedium to 70-140 mesh, and pouring into No. 1 medicine solution; S32, first pour tangerine peel, green leafy vegetables and epimedium into the liquid medicine, and boil the liquid medicine over high heat; S33. Then add brown sugar, turn to low heat and simmer for 30-55 minutes. Meanwhile, stir continuously during the simmering process to obtain medicinal solution No.

2.

9. The method for preparing a Chinese medicine composition for preventing female ovulation dysfunction according to claim 8, characterized in that: The step of concentrating the decoction in S5 to obtain No. 1 extract comprises the following steps: S51, concentrating the decoction to a liquid having a relative density of 1.1-1.2 at 70-85°C; S52, after being allowed to cool to room temperature, alcohol precipitation treatment is performed; S53, then let it stand and take out the supernatant to concentrate it to a No. 1 extract with a relative density of 1.2-1.3 at 70-85°C.

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