Humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco and preparation method thereof

Abstract

The invention discloses a humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco and a preparation method of the humic acid type phosphate fertilizer synergist, and relates to the technical field of synergist preparation. The humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco comprises the following raw materials in parts by weight: 20-100 parts of biomass, 10-50 parts of turfy soil, 1-6 parts of a microbial agent, 2-5 parts of calcium superphosphate, 1-3 parts of a complexing agent, 12-35 parts of humic acid, 5-10 parts of a fixing agent, 3-6 parts of a water-retaining agent and 10-30 parts of urea. By additionally adding the fixing agent and the microbial agent, water retention and water locking are carried out on soil, the physical state of the soil is improved, the enzyme activity of the soil is improved, various biochemical reactions in the soil can be catalyzed, the soil fertility is improved, and the seepage force and the adhesive force of a phosphate fertilizer entering a soil layer are enhanced; calcium phosphate and a complexing agent are combined with soil metal ions, so that the content of the phosphate fertilizer in the soil is further increased.

Description

Technical Field

[0001] The invention relates to the technical field of synergist preparation, in particular to a humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco and a preparation method thereof. Background Art

[0002] Fertilizer scientists have conducted a lot of research on ways and methods to improve fertilizer utilization, and have achieved certain results. However, there are still problems such as complex fertilizer production processes, high costs, inconvenient use, and difficulty in promoting and applying fertilizers on field crops. In recent years, how to upgrade the fertilizer industry with low-cost investment has become a research hotspot. In the past few decades, there have been many studies on fertilizers, especially nitrogen fertilizer enhancers. These studies have mostly focused on the research and application of chemical agents such as urease inhibitors and nitrification inhibitors, and there have been few studies on the application of natural substances and modified natural substances in fertilizer enhancers.

[0003] For example, a modified humic acid fertilizer synergist, and its production method and use disclosed in Chinese invention patent publication number CN102701868A is prepared by utilizing weathered coal, lignite, sodium humate or potassium humate and trace elements to improve nutrient utilization, increase crop yield, improve agricultural product quality, reduce nutrient loss, avoid secondary pollution, reduce agricultural production costs, and supplement soil trace elements. It has the characteristics of wide raw material sources, low cost, simple production process, and convenient use in fertilizer production, and has broad application prospects in synergistic production.

[0004] The above-mentioned enhancer is prepared by weathered coal, lignite, sodium humate or potassium humate and trace elements to prepare modified humic acid fertilizer enhancer, so as to supplement the trace elements in the soil. However, the above-mentioned enhancer is difficult to improve the physical properties of the soil layer where flue-cured tobacco leaves are planted. It is difficult to improve the planting environment and ecological environment of flue-cured tobacco leaves in cultivated land by only increasing the development and growth of plants from the effective phosphorus content in the soil. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco and a preparation method thereof, which solves the problems raised in the above-mentioned background technology.

[0006] To achieve the above objectives, the present invention is implemented through the following technical scheme: a humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco and a preparation method thereof, comprising the following raw material ratios in parts by weight: 20-100 parts of biomass, 10-50 parts of peat soil, 1-6 parts of microbial agent, 2-5 parts of superphosphate, 1-3 parts of chelating agent, 12-35 parts of humic acid, 5-10 parts of fixing agent, 3-6 parts of water retaining agent, and 10-30 parts of urea.

[0007] The further improvement of the technical solution of the present invention is that: the biomass is selected as straw powder or sawdust powder or other agricultural waste. The humic acid is prepared from a certain amount of weathered coal, dilute hydrochloric acid and sodium hydroxide solution.

[0008] The further improvement of the technical solution of the present invention is that the raw materials of the fixing agent are selected as high-purity sodium α-naphthylacetate and ammonium ethyl hexanoate.

[0009] A further improvement of the technical solution of the present invention is that the water retaining agent is carboxymethyl starch and the complexing agent is tetrasodium glutamate diacetate.

[0010] A further improvement of the technical solution of the present invention is that the following specific steps are taken to prepare the microbial agent suitable for flue-cured tobacco:

[0011] Q1: According to the PDA medium formula, put the potatoes cut into small pieces into the pot one by one, add 1000ml of water, use a heating device to heat the pot to boiling state and maintain for 20-40min, use two layers of sterile gauze to filter on a measuring cup while hot, add water to the filtrate to 1000ml, put the filtered potato liquid back into the pot, add glucose and agar, use the heating device again to heat and stir, after the agar is completely dissolved, add water at least 1000ml to obtain the PDA medium;

[0012] Q2: After activating the strain of Corymborhinus annuus using the PDA medium obtained in Q1, the strain was placed in an incubator at a constant temperature of 25°C and cultured without light for 12 hours, and then cultured under natural light for 1 hour to induce the strain of Corymborhinus annuus to produce spores. The produced spores were placed in a centrifugal device for oscillation for 5 minutes, and the spore suspension was obtained after filtering with sterile gauze;

[0013] Q3: The obtained spore suspension of the Cyclosporon spore strain was mixed with the PDA medium obtained in Q1 according to the weight ratio, and cultured at a temperature maintained at 20-30°C for 20 hours until white mycelium appeared on the PDA medium, thereby obtaining the microbial agent used for the preparation of the humic acid type phosphate fertilizer enhancer.

[0014] A further improvement of the technical solution of the present invention is that the preparation of the humic acid type phosphate fertilizer synergist comprises the following specific steps:

[0015] S1: Raw material preparation; prepare a certain amount of biomass, peat soil, microbial agent, superphosphate, chelating agent, humic acid, fixing agent, water retaining agent, and urea according to the ratio, wherein the biomass is selected from straw powder or sawdust powder, the fixing agent raw material is selected from high-purity sodium α-naphthylacetate and aminoethyl ester, the water retaining agent is selected from carboxymethyl starch, the chelating agent is selected from tetrasodium glutamate diacetate, and the microbial agent is selected from Cyclosporum cyclosporum;

[0016] S2: Raw material processing; the straw powder is dried and ventilated in a vacuum rake dryer and then crushed into particles with a diameter not exceeding 2 mm by a roller mill. The bottom of the fermentation tank is paved with peat soil, and 45% of the obtained powder particles are evenly spread in the fermentation tank;

[0017] S3: Fermentation preparation: add calcium phosphate and a fixative to the remaining 55% of the biomass powder particles in S2, stir, pour urea evenly during the stirring process, pile up for fermentation, stir daily, add to a roller mill for secondary crushing, add the microbial agent prepared in S1 during the crushing process, and pour the resulting product into the fermentation tank in S2;

[0018] S4: granulation; after compacting the fermentation substrate, the fermentation tank is capped with a polyvinyl chloride film, and the fermentation is cultured until the moisture content of the fermented product is less than 2.5% to obtain fermented straw powder. At this time, a chelating agent, humic acid, a water retaining agent, and a fixing agent are added and stirred for 50 minutes to obtain fermented straw powder containing humic acid and a chelating agent. At this time, the powder is passed into a disc granulator for granulation, and finally a humic acid-type phosphate fertilizer synergist with a particle size of less than 1.5 mm is obtained through screening.

[0019] A further improvement of the technical solution of the present invention is that: the fixing agent is prepared by selecting 25-50 parts of high-purity sodium α-naphthylacetate and 10-20 parts of ammonium ethyl ester for 5-10 parts of the fixing agent according to the raw material ratio in parts by weight, and 65-150 parts of carbonate rock are added as an auxiliary.

[0020] A further improvement of the technical solution of the present invention is that the preparation of the fixing agent also includes the following specific steps: ammonium ethyl hexanoate and high-purity sodium α-naphthylacetate are mixed in proportion, then the carbonate rock is sieved through a 1000-mesh sieve, the mixed ammonium ethyl hexanoate and high-purity sodium α-naphthylacetate are mixed with the carbonate rock that has been preliminarily screened and crushed, and carboxylic acid and sulfonic acid are added to form the desired fixing agent.

[0021] A further improvement of the technical solution of the present invention is that the humic acid is prepared from a certain amount of weathered coal, dilute hydrochloric acid and sodium hydroxide solution.

[0022] A further improvement of the technical solution of the present invention is that the humic acid comprises the following specific steps: the dried weathered coal is crushed by a double-roll mill and then passed through 100 meshes, and a certain amount is weighed and put into a beaker, and then 2% dilute hydrochloric acid is added and heated and boiled for 20 minutes, and then filtered and washed for desalination, and the precipitate is transferred to the beaker, and a certain concentration of sodium hydroxide solution is added and stirred evenly, and after boiling, it is allowed to stand for stratification, and the humic acid is separated from the impurities by centrifugation, and the separated solution is filtered and washed with pure water, and then dried and crushed, and passed through 200 meshes to obtain humic acid.

[0023] The present invention provides a humic acid-type phosphate fertilizer synergist suitable for flue-cured tobacco and a preparation method thereof. Compared with the prior art, the synergist has the following beneficial effects: by using humic acid to improve the status of organic matter and other nutrient elements in the soil, and providing a relatively ideal soil environment for the metabolism and life activities of soil bacteria, additional fixatives and microbial agents are added to retain water in the soil, improve the physical state of the soil, increase the activity of soil enzymes, help catalyze various biochemical reactions in the soil, improve soil fertility, and enhance the penetration and adhesion of phosphate fertilizers into the soil layer, and further increase the content of phosphate fertilizers in the soil by combining calcium phosphate and chelating agents with soil metal ions;

[0024] The correlation analysis between the dosage of humic acid, fixative and microbial agent and soil enzymes showed that humic acid was positively correlated with sucrase and phosphatase, which improved the content of related enzymes in the soil, thereby further improving the planting environment and ecological environment of flue-cured tobacco leaves in cultivated land. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a flowchart of the preparation of the humic acid type phosphate fertilizer synergist for flue-cured tobacco and the humic acid type phosphate fertilizer synergist in the preparation method thereof;

[0026] Figure 2 This is a flowchart of the preparation of the humic acid type phosphate fertilizer synergist for flue-cured tobacco and the microbial agent in the preparation method thereof;

[0027] Figure 3 A line graph showing the effect of the humic acid-based phosphate fertilizer synergist for flue-cured tobacco and the synergist in its preparation method on the microorganisms of the soil flora in the plough layer;

[0028] Figure 4 A line graph showing the effect of the humic acid-based phosphate fertilizer synergist for flue-cured tobacco and the synergist in its preparation method on soil enzyme activity;

[0029] Figure 5 The present invention is a bar graph showing the influence of the humic acid type phosphate fertilizer synergist for flue-cured tobacco and the synergist in its preparation method on the chemical components of flue-cured tobacco leaves. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Reference Figure 1-Figure 5 , the present invention provides 4 technical solutions:

[0032] Embodiment 1:

[0033] A humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco and a preparation method thereof, comprising the following raw material ratios in parts by weight: 20-100 parts of biomass, 10-50 parts of peat soil, 1-6 parts of microbial agent, 2-5 parts of superphosphate, 1-3 parts of chelating agent, 12-35 parts of humic acid, 5-10 parts of fixing agent, 3-6 parts of water retaining agent and 10-30 parts of urea.

[0034] The biomass is selected to be straw powder or sawdust powder or other agricultural waste. The humic acid is prepared from a certain amount of weathered coal, dilute hydrochloric acid and sodium hydroxide solution.

[0035] The fixing agent raw materials are selected from high-purity sodium α-naphthylacetate and ammonium ethyl ester.

[0036] The water retaining agent is carboxymethyl starch, and the complexing agent is tetrasodium glutamate diacetate.

[0037] Embodiment 2: Based on Embodiment 1, the present invention further provides the steps for preparing the microbial agent mentioned in Embodiment 1, specifically:

[0038] Q1: According to the PDA medium formula, put the potatoes cut into small pieces into the pot one by one, add 1000ml of water, use a heating device to heat the pot to boiling state and maintain for 20-40min, use two layers of sterile gauze to filter on a measuring cup while hot, add water to the filtrate to 1000ml, put the filtered potato liquid back into the pot, add glucose and agar, use the heating device again to heat and stir, after the agar is completely dissolved, add water at least 1000ml to obtain the PDA medium;

[0039] Q2: After activating the strain of Corymborhinus annuus using the PDA medium obtained in Q1, the strain was placed in an incubator at a constant temperature of 25°C and cultured without light for 12 hours, and then cultured under natural light for 1 hour to induce the strain of Corymborhinus annuus to produce spores. The produced spores were placed in a centrifugal device for oscillation for 5 minutes, and the spore suspension was obtained after filtering with sterile gauze;

[0040] Q3: The obtained spore suspension of the Cyclosporon spore strain was mixed with the PDA medium obtained in Q1 according to the weight ratio, and cultured at a temperature maintained at 20-30°C for 20 hours until white mycelium appeared on the PDA medium, thereby obtaining the microbial agent used for the preparation of the humic acid type phosphate fertilizer enhancer.

[0041] Embodiment 3: Based on Embodiment 1 and Embodiment 2, the present invention further provides a method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco, comprising the following specific steps:

[0042] S1: Raw material preparation; prepare a certain amount of biomass, peat soil, microbial agent, superphosphate, chelating agent, humic acid, fixing agent, water retaining agent, and urea according to the ratio, wherein the biomass is selected from straw powder or sawdust powder, the fixing agent raw material is selected from high-purity sodium α-naphthylacetate and aminoethyl ester, the water retaining agent is selected from carboxymethyl starch, the chelating agent is selected from tetrasodium glutamate diacetate, and the microbial agent is selected from Cyclosporum cyclosporum;

[0043] S2: Raw material processing; the straw powder is dried and ventilated in a vacuum rake dryer and then crushed into particles with a diameter not exceeding 2 mm by a roller mill. The bottom of the fermentation tank is paved with peat soil, and 45% of the obtained powder particles are evenly spread in the fermentation tank;

[0044] S3: Fermentation preparation: add calcium phosphate and a fixative to the remaining 55% of the biomass powder particles in S2, stir, pour urea evenly during the stirring process, pile up for fermentation, stir daily, add to a roller mill for secondary crushing, add the microbial agent prepared in S1 during the crushing process, and pour the resulting product into the fermentation tank in S2;

[0045] S4: granulation; after compacting the fermentation substrate, the fermentation tank is capped with a polyvinyl chloride film, and the fermentation is cultured until the moisture content of the fermented product is less than 2.5% to obtain fermented straw powder. At this time, a chelating agent, humic acid, a water retaining agent, and a fixing agent are added and stirred for 50 minutes to obtain fermented straw powder containing humic acid and a chelating agent. At this time, the powder is passed into a disc granulator for granulation, and finally a humic acid-type phosphate fertilizer synergist with a particle size of less than 1.5 mm is obtained through screening.

[0046] The fixing agent is prepared by selecting 25-50 parts of high-purity sodium α-naphthylacetate and 10-20 parts of ammonium ethyl ester for 5-10 parts of the fixing agent according to the raw material ratio in parts by weight, and 65-150 parts of carbonate rock are added as an auxiliary.

[0047] The preparation of the fixing agent also includes the following specific steps: mixing ammonium ethyl ester and high-purity sodium α-naphthylacetate in proportion, then passing carbonate rock through a 1000-mesh sieve, mixing the mixed ammonium ethyl ester and high-purity sodium α-naphthylacetate with the carbonate rock that has been preliminarily screened and crushed, and adding carboxylic acid and sulfonic acid to form the desired fixing agent.

[0048] The humic acid is prepared from a certain amount of weathered coal, dilute hydrochloric acid and sodium hydroxide solution; the humic acid comprises the following specific steps: the dried weathered coal is crushed by a double-roll mill and then passed through 100 meshes, and a certain amount is weighed and put into a beaker, and then 2% dilute hydrochloric acid is added and heated and boiled for 20 minutes, and then filtered and washed for desalination, and the precipitate is transferred to the beaker, and then a certain concentration of sodium hydroxide solution is added and stirred evenly, and after boiling, it is allowed to stand for stratification, and the humic acid is separated from impurities by centrifugation, and the separated solution is filtered and washed with pure water, and then dried and crushed, and passed through 200 meshes to obtain humic acid.

[0049] Example 4: For the humic acid type phosphate fertilizer synergist obtained in Example 3, this example uses a unified test field to conduct a control verification experiment. The test soil is brown soil, and the soil pH value of the test is measured to be 6.98;

[0050] The control group did not apply humic acid, so the raw material ratio by weight was 50 parts of biomass, 25 parts of peat soil, 3 parts of microbial agent, 3 parts of superphosphate, 2 parts of complexing agent, 8 parts of fixing agent, 4 parts of water retaining agent, and 15 parts of urea;

[0051] The specific operation is as follows: prepare a certain amount of biomass, peat soil, microbial agent, superphosphate, chelating agent, fixing agent, water retaining agent, and urea according to the ratio, wherein the biomass is selected from straw powder or sawdust powder, the fixing agent raw materials are selected from high-purity sodium α-naphthylacetate and aminoethyl ester, the water retaining agent is selected from carboxymethyl starch, the chelating agent is selected from tetrasodium glutamate diacetate, and the microbial agent is selected from Cyclosporum cyclosporum; dry and ventilate the straw powder in a vacuum rake dryer, and then use a double-roll mill to crush it into particles with a diameter not exceeding 2mm, and then lay peat soil on the bottom of the fermentation tank, and 45% of the obtained powder particles are evenly spread in the fermentation tank; the remaining 55% of the biomass Calcium phosphate and a fixing agent are added to the powder particles, and the mixture is stirred. During the stirring process, urea is evenly poured into the powder particles, and the powder particles are piled for fermentation. The powder particles are stirred every day, and then the powder particles are added into a roller mill for secondary crushing. During the crushing process, a prepared microbial agent is added, and the obtained product is poured into a fermentation tank. After the fermentation substrate is compacted, the fermentation tank is capped with a polyvinyl chloride film, and the fermentation is cultured until the water content of the fermented product is less than 2.5% to obtain fermented straw powder. At this time, a complexing agent, a water retaining agent and a fixing agent are added, and the mixture is stirred for 50 minutes to obtain fermented straw powder with a complexing agent. At this time, the powder particles are passed into a disc granulator for granulation, and finally a phosphate fertilizer enhancer with a particle size of less than 1.5 mm is obtained through screening.

[0052] In the experimental group 1, humic acid was applied, and the raw material ratio by weight was 50 parts of biomass, 25 parts of peat soil, 3 parts of microbial agent, 3 parts of superphosphate, 2 parts of chelating agent, 12 parts of humic acid, 8 parts of fixing agent, 4 parts of water retaining agent, and 15 parts of urea;

[0053] In the experimental group 2, humic acid was applied, and the raw material ratio by weight was 50 parts of biomass, 25 parts of peat soil, 3 parts of microbial agent, 3 parts of superphosphate, 2 parts of chelating agent, 24 parts of humic acid, 8 parts of fixing agent, 4 parts of water retaining agent, and 15 parts of urea;

[0054] In the experimental group 3, humic acid was applied, and the raw material ratio by weight was 50 parts of biomass, 25 parts of peat soil, 3 parts of microbial agent, 3 parts of superphosphate, 2 parts of chelating agent, 35 parts of humic acid, 8 parts of fixing agent, 4 parts of water retaining agent, and 15 parts of urea;

[0055] In the experimental group 4, humic acid was applied, and the raw material ratio by weight was 50 parts of biomass, 25 parts of peat soil, 3 parts of microbial agent, 3 parts of superphosphate, 2 parts of chelating agent, 40 parts of humic acid, 8 parts of fixing agent, 4 parts of water retaining agent, and 15 parts of urea;

[0056] In the experimental group 5, humic acid was applied, and the raw material ratio by weight was 50 parts of biomass, 25 parts of peat soil, 3 parts of superphosphate, 2 parts of chelating agent, 35 parts of humic acid, 4 parts of water retaining agent, and 15 parts of urea;

[0057] The specific operations used in the experimental groups 1 to 4 are as follows: prepare a certain amount of biomass, peat soil, microbial agent, superphosphate, chelating agent, humic acid, water retaining agent, and urea according to the ratio, wherein the biomass is selected from straw powder or sawdust powder, the fixing agent raw material is selected from high-purity sodium α-naphthylacetate and aminoethyl ester, the water retaining agent is selected from carboxymethyl starch, the chelating agent is selected from tetrasodium glutamate diacetate, and the microbial agent is selected from Cyclosporum cyclosporum; the straw powder is dried and ventilated in a vacuum rake dryer and is in a dry state, and then it is crushed into particles with a diameter of no more than 2 mm by a double-roll mill, and peat soil is laid on the bottom of the fermentation tank, and 45% of the obtained powder particles are evenly spread in the fermentation tank; the remaining 55% of the biomass in S2 is Calcium phosphate and a fixing agent are added to the powder particles and stirred. During the stirring process, urea is evenly poured in, piled for fermentation, and stirred every day. The powder particles are then added to a roller mill for secondary crushing. During the crushing process, a prepared microbial agent is added, and the obtained product is poured into a fermentation tank in S2. After the fermentation substrate is compacted, the fermentation tank is capped with a polyvinyl chloride film, and the fermentation is cultured until the water content of the fermented product is less than 2.5% to obtain fermented straw powder. At this time, a chelating agent, humic acid, a water retaining agent, and a fixing agent are added and stirred for 50 minutes to obtain fermented straw powder containing humic acid and a chelating agent. At this time, the powder is passed into a disc granulator for granulation, and finally a humic acid-type phosphate fertilizer enhancer with a particle size of less than 1.5 mm is obtained through screening.

[0058] The specific operation used in the experimental group 5 is as follows: prepare a certain amount of biomass, peat soil, superphosphate, water retaining agent, and urea according to the ratio, wherein the biomass is selected from straw powder or sawdust powder, and the water retaining agent is selected from carboxymethyl starch; the straw powder is dried and ventilated in a vacuum rake dryer in a dry state, and then crushed into particles with a diameter of no more than 2 mm by a double-roll mill, and peat soil is laid on the bottom of the fermentation tank, and 45% of the obtained powder particles are evenly spread in the fermentation tank; the remaining 55% of the biomass powder particles are added and stirred, and poured Urea is added, piled for fermentation, and the mixture is stirred every day. The mixture is then added to a roller mill for secondary crushing. The resulting product is poured into a fermentation tank. After the fermentation substrate is compacted, the fermentation tank is capped with a polyvinyl chloride film. The fermentation is cultured until the water content of the fermented product is less than 2.5% to obtain fine fermented straw powder. At this time, a complexing agent and a water retaining agent are added and stirred for 50 minutes to obtain fine fermented straw powder containing a complexing agent. The powder is then passed into a disc granulator for granulation, and finally a phosphate fertilizer synergist with a particle size of less than 1.5 mm is obtained through screening.

[0059] The control group and each experimental group were treated with 100m 2 of flue-cured tobacco, and all of them were used as basal fertilizer. The transplanting period was the same. Other measures were carried out according to the normal production management of flue-cured tobacco leaves and were the same for each group. After 50 days of flue-cured tobacco production, 100m of each treatment was collected by random multi-point sampling method. 2 1 kg of soil sample from the inner flue-cured tobacco plough layer was air-dried and ground for later use, and 1 kg of tobacco leaves from the middle were collected for testing;

[0060] The bacteria, fungi, actinomycetes, phosphorus or potassium bacteria, oxidizing bacteria, etc. contained in the collected soil were determined by plate counting method, the sucrase in the soil was determined by colorimetry and fluorescence method, the phosphatase was determined by disodium phenyl phosphate colorimetry, the total nitrogen in the collected tobacco leaves was determined by sulfuric acid-mixed accelerator-distillation method, the total sugar and starch were determined by acid hydrolysis method, and the nicotine was determined by gas chromatography;

[0061] Analyze the measured data, as shown in the attached Figure 3 Effects of humic acid on soil microflora shown;

[0062] By the attached Figure 3 As shown, after applying humic acid, fixative and microbial agents, the number of bacteria, fungi, actinomycetes, phosphorus bacteria, potassium bacteria and ammoniation bacteria in the soil increased to varying degrees compared with the control group; compared with the control group, it was shown that applying a certain amount of humic acid, fixative and microbial agents can promote the reproduction and development of soil bacteria, fungi, actinomycetes, phosphorus bacteria, potassium bacteria and ammoniation bacteria, which is beneficial to the decomposition and transformation of soil organic matter and the effectiveness of mineral nutrients, while in the experimental group 4, it was revealed that applying excessive humic acid would hinder the reproduction and development of soil bacteria, fungi, actinomycetes, phosphorus bacteria, potassium bacteria and ammoniation bacteria;

[0063] Analyze the measured data, as shown in the attached Figure 4 Effects of humic acid on soil enzymes shown;

[0064] By the attached Figure 4 As shown in the figure, the correlation analysis between the dosage of humic acid, fixative and microbial agent and soil enzymes showed that humic acid was positively correlated with sucrase and phosphatase, but with the continuous addition of humic acid, urease was inhibited. It can be seen that applying a certain amount of humic acid, fixative and microbial agent can increase soil enzyme activity, thereby helping to catalyze various biochemical reactions in the soil, improve soil fertility, and promote the absorption of phosphorus fertilizer by flue-cured tobacco leaves.

[0065] Analyze the measured data, as shown in the attached Figure 4 The effects of humic acid on the chemical composition of flue-cured tobacco leaves are shown;

[0066] By the attached Figure 5 As shown in the results, the application of humic acid, fixative and microbial agent reduced the content of nicotine and total nitrogen, and the starch content also tended to decrease. After the application of humic acid, the reducing sugar and total sugar of flue-cured tobacco leaves showed an upward trend compared with the control group, revealing that humic acid can increase the content of total sugar and reducing sugar in tobacco leaves, and increase the ratio of two sugars in tobacco leaves; reduce the content of nicotine, total nitrogen and starch. Humic acid can make the chemical composition of flue-cured tobacco leaves more coordinated during their growth and development.

[0067] In summary, the experimental group 2 in this embodiment is the best experimental method of this embodiment; the raw material ratio by weight is 50 parts of biomass, 25 parts of peat soil, 3 parts of microbial agent, 3 parts of superphosphate, 2 parts of chelating agent, 24 parts of humic acid, 8 parts of fixing agent, 4 parts of water retaining agent, and 15 parts of urea;

[0068] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited, and conventional equipment can be used.

[0069] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0070] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A humic acid type phosphate fertilizer synergist suitable for flue-cured tobacco, characterized in that: The invention comprises the following raw material proportions in parts by weight: 20-100 parts of biomass, 10-50 parts of peat soil, 1-6 parts of microbial agent, 2-5 parts of superphosphate, 1-3 parts of chelating agent, 12-35 parts of humic acid, 5-10 parts of fixing agent, 3-6 parts of water retaining agent and 10-30 parts of urea.

2. A humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 1, characterized in that: The biomass is selected to be straw powder or sawdust powder or other agricultural waste. The humic acid is prepared from a certain amount of weathered coal, dilute hydrochloric acid and sodium hydroxide solution.

3. The humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 1, characterized in that: The fixing agent raw materials are selected from high-purity sodium α-naphthylacetate and ammonium ethyl ester.

4. The humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 1, characterized in that: The water retaining agent is carboxymethyl starch, and the complexing agent is tetrasodium glutamate diacetate.

5. The method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 1, characterized in that: The following specific steps are taken to prepare the microbial agent suitable for flue-cured tobacco: Q1: According to the PDA medium formula, put the potatoes cut into small pieces into the pot one by one, add 1000ml of water, use a heating device to heat the pot to boiling state and maintain for 20-40min, use two layers of sterile gauze to filter on a measuring cup while hot, add water to the filtrate to 1000ml, put the filtered potato liquid back into the pot, add glucose and agar, use the heating device again to heat and stir, after the agar is completely dissolved, add water at least 1000ml to obtain the PDA medium; Q2: After activating the strain of Corymborhinus annuus using the PDA medium obtained in Q1, the strain was placed in an incubator at a constant temperature of 25°C and cultured without light for 12 hours, and then cultured under natural light for 1 hour to induce the strain of Corymborhinus annuus to produce spores. The produced spores were placed in a centrifugal device for oscillation for 5 minutes, and the spore suspension was obtained after filtering with sterile gauze; Q3: The obtained spore suspension of the Cyclosporon spore strain was mixed with the PDA medium obtained in Q1 according to the weight ratio, and cultured at a temperature maintained at 20-30°C for 20 hours until white mycelium appeared on the PDA medium, thereby obtaining the microbial agent used for the preparation of the humic acid type phosphate fertilizer enhancer.

6. The method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 1, characterized in that: The preparation of the humic acid type phosphate fertilizer synergist comprises the following specific steps: S1: Raw material preparation; prepare a certain amount of biomass, peat soil, microbial agent, superphosphate, chelating agent, humic acid, fixing agent, water retaining agent, and urea according to the ratio, wherein the biomass is selected from straw powder or sawdust powder, the fixing agent raw material is selected from high-purity sodium α-naphthylacetate and aminoethyl ester, the water retaining agent is selected from carboxymethyl starch, the chelating agent is selected from tetrasodium glutamate diacetate, and the microbial agent is selected from Cyclosporum cyclosporum; S2: Raw material processing; The straw powder is dried and ventilated in a vacuum rake dryer and then crushed into particles with a diameter of no more than 2 mm by a roller mill. The bottom of the fermentation tank is paved with peat soil, and 45% of the obtained powder particles are evenly spread in the fermentation tank. S3: Fermentation preparation: add calcium phosphate and a fixative to the remaining 55% of the biomass powder particles in S2, stir, pour urea evenly during the stirring process, pile up for fermentation, stir daily, add to a roller mill for secondary crushing, add the microbial agent prepared in S1 during the crushing process, and pour the resulting product into the fermentation tank in S2; S4: granulation; after compacting the fermentation substrate, the fermentation tank is capped with a polyvinyl chloride film, and the fermentation is cultured until the moisture content of the fermented product is less than 2.5% to obtain fermented straw powder. At this time, a chelating agent, humic acid, a water retaining agent, and a fixing agent are added and stirred for 50 minutes to obtain fermented straw powder containing humic acid and a chelating agent. At this time, the powder is passed into a disc granulator for granulation, and finally a humic acid-type phosphate fertilizer synergist with a particle size of less than 1.5 mm is obtained through screening.

7. The method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 6, characterized in that: The fixing agent is prepared by selecting 25-50 parts of high-purity sodium α-naphthylacetate and 10-20 parts of ammonium ethyl ester for 5-10 parts of the fixing agent according to the raw material ratio in parts by weight, and 65-150 parts of carbonate rock are added as an auxiliary.

8. The method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 7, characterized in that: The preparation of the fixing agent also includes the following specific steps: mixing ammonium ethyl ester and high-purity sodium α-naphthylacetate in proportion, then passing carbonate rock through a 1000-mesh sieve, mixing the mixed ammonium ethyl ester and high-purity sodium α-naphthylacetate with the carbonate rock that has been preliminarily screened and crushed, and adding carboxylic acid and sulfonic acid to form the desired fixing agent.

9. The method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 6, characterized in that: The humic acid is prepared from a certain amount of weathered coal, dilute hydrochloric acid and sodium hydroxide solution.

10. The method for preparing a humic acid-based phosphate fertilizer synergist suitable for flue-cured tobacco according to claim 9, characterized in that: The humic acid production method comprises the following specific steps: the dried weathered coal is crushed by a double-roll crusher and then passed through 100 meshes, and a certain amount is weighed and put into a beaker, and then 2% dilute hydrochloric acid is added and heated and boiled for 20 minutes, and then filtered and washed for desalination, and the precipitate is transferred to the beaker, and then a certain concentration of sodium hydroxide solution is added and stirred evenly, and after boiling, it is allowed to stand for stratification, and the humic acid is separated from the impurities by centrifugation, and the separated solution is filtered and washed with pure water, and then dried and crushed, and passed through 200 meshes to obtain the humic acid.

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