Embryo culture device capable of changing air and liquid and simulating environment in uterus

Abstract

The invention relates to an embryo culture device capable of changing air and liquid for simulating an intrauterine environment, which comprises a motion culture platform and an embryo culture bag arranged in the motion culture platform, the motion culture platform comprises a culture platform, a motion support structure connected below the culture platform and a control host arranged below the motion support structure, the control host controls the culture platform to move regularly or irregularly, the embryo culture bag comprises a bag body and a ventilation pipeline and a liquid changing pipeline which are connected in the bag body, the embryo culture bag is placed in the culture platform, a bag body fixing structure used for fixing the embryo culture bag is arranged in the culture platform, and a placing groove is formed in the upper surface of the culture platform. Heating modules are arranged at the bottoms of the containing grooves. The device is small in size, state adjustment is convenient, four-axis shake-up movement in the random direction is achieved, the embryo movement direction is not fixed, the device is closer to a maternal culture environment, the movement mode of an embryo in the uterus is simulated, and the embryo is not limited by the size of a culture container.

Description

Technical Field

[0001] The present application relates to the field of embryo culture technology, and in particular to a ventilation and fluid exchangeable embryo culture device that simulates the intrauterine environment. Background Art

[0002] Embryo in vitro culture is to place the embryo in a special container at 37°C to allow the embryo to continue to develop outside the body. The requirements for culture medium and sterility are relatively strict. This method is often used to identify embryo survival rates and conduct embryo in vitro research.

[0003] Embryo culture requires the use of professional embryo culture equipment. However, existing embryo culture equipment is large in size and inconvenient to adjust. In addition, the culture container is made of glass, and there is elastic collision between the embryo and the container. Once a collision occurs, the embryo will be greatly affected. Summary of the invention

[0004] The purpose of the present application is to provide a ventilation and fluid-exchange embryo culture device that simulates the intrauterine environment. The device is small in size, convenient for state adjustment, and has four-axis random direction shaking motion. The embryo movement direction is not fixed, which is closer to the maternal culture environment and simulates the embryo's movement pattern in the uterus. The embryo is not limited by the volume of the culture container.

[0005] The present application provides a ventilation and fluid exchangeable embryo culture device that simulates the intrauterine environment and adopts the following technical solutions:

[0006] A ventilation and fluid exchange embryo culture device that simulates the intrauterine environment comprises a motion culture platform and an embryo culture bag arranged in the motion culture platform. The motion culture platform comprises a culture platform, a motion support structure connected to the bottom of the culture platform, and a control host arranged under the motion support structure. The control host controls the regular or irregular movement of the culture platform. The embryo culture bag comprises a bag body and a ventilation pipeline and a fluid exchange pipeline connected to the bag body. The embryo culture bag is placed in the culture platform, and a bag body fixing structure for fixing the embryo culture bag is arranged in the culture platform.

[0007] As a preferred technical solution of the present application, a placement groove is arranged on the upper surface of the culture platform, and a heating module is arranged at the bottom of the placement groove.

[0008] As a preferred technical solution of the present application, a balanced display module is provided on the upper surface of the culture platform.

[0009] As a preferred technical solution of the present application, the motion support structure is configured as a four-axis motion platform, and the culture platform is installed on the motion table of the motion support structure.

[0010] As a preferred technical solution of the present application, the control host includes a casing body with a control system arranged inside, and a complete machine switch, control knob and display screen arranged on the casing body, and the complete machine switch, control knob and display screen are all connected to the control system.

[0011] As a preferred technical solution of the present application, the bag body fixing structure is provided with two groups in the placement groove, and the bag body fixing structure includes two support columns fixed on the bottom surface of the placement groove and a bag body fixing splint connected between the two support columns, one end of the bag body fixing splint is rotatably connected to one support column, and the other end is bolted to the other support column.

[0012] As a preferred technical solution of the present application, the bag body fixing splint is U-shaped, with connecting parts connected to the support columns provided at both ends, and the bottom of the U-shaped structure of the bag body fixing splint is used to abut the bottom surface of the placement groove.

[0013] As a preferred technical solution of the present application, a pipeline mounting seat and a sensor interface are provided on the upper side of the bag body, and the ventilation pipeline and the liquid exchange pipeline are connected to the bag body through the pipeline mounting seat.

[0014] As a preferred technical solution of the present application, the ventilation pipeline includes an air inlet pipe and an air outlet pipe, a filter is provided on the air inlet pipe, and a shut-off clamp is provided on both the air inlet pipe and the air outlet pipe.

[0015] As a preferred technical solution of the present application, the liquid exchange pipeline includes a liquid inlet pipe and a liquid outlet pipe, a filter is provided on the liquid inlet pipe, and a shut-off clamp is provided on both the liquid inlet pipe and the liquid outlet pipe.

[0016] In summary, the present application includes at least one of the following beneficial technical effects:

[0017] 1. The embryo culture device of the present application is small in size, occupies little space, and is easy to move and install.

[0018] 2. The device of the present application is convenient for automatic leveling, providing a better environment for embryo culture.

[0019] 3. The device of the present application can set parameters such as mixing frequency, angle, time, etc., making it more convenient to use.

[0020] 4. The device of the present application can detect various relevant parameters in the culture medium, not limited to pH value and dissolved oxygen, etc., which is convenient for observing changes in the embryo culture environment.

[0021] 5. The device of the present application adopts four-axis random direction shaking motion, the embryo movement direction is not fixed, which is closer to the maternal culture environment, simulating the movement pattern of the embryo in the uterus, and the embryo is not limited by the volume of the culture container.

[0022] 6. The culture container is a special culture bag made of soft material, which can realize aseptic replacement of culture medium. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application;

[0024] Figure 2 It is a front view of an embodiment of the present application;

[0025] Figure 3 is a top view of an embodiment of the present application;

[0026] Figure 4 This is a schematic diagram of the structure of the embryo culture bag in the embodiment of the present application;

[0027] In the figure, 1. culture platform; 10. casing body; 11. placement slot; 2. motion support structure; 3. whole machine switch; 4. control knob; 5. display screen; 6. heating module; 7. balance display module; 8. bag body; 81. pipeline mounting seat; 82. sensor interface; 83. air inlet pipe; 84. air outlet pipe; 85. filter; 86. interception clamp; 87. liquid inlet pipe; 88. liquid outlet pipe; 9. bag body fixing structure; 91. support column; 92. bag body fixing splint. DETAILED DESCRIPTION

[0028] The following is combined with Figure 1 -Attached Figure 4 , further details of this application are given.

[0029] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0030] Embodiment: This application proposes a ventilation and fluid exchangeable embryo culture device that simulates the intrauterine environment, referring to Figure 1-4 The device includes two structures: a motion culture platform and an embryo culture bag. The embryo culture bag is used to place and culture embryos, simulating the movement pattern of embryos in the uterus. The embryo culture bag is set in the motion culture platform, and the motion culture platform drives the embryo culture bag to move.

[0031] The motion culture platform includes a culture platform 1, a motion support structure 2 and a control host. The culture platform 1 is a rectangular plane plate structure as a whole, and a rectangular placement groove 11 is provided on the upper surface. A heating module 6 is laid at the bottom of the placement groove 11. The heating module 6 can be a heating plate or a heating tube, which is used to heat the embryo culture bag to make the culture temperature closer to the uterine temperature in the mother's body. A balance display module 7 is provided on the upper surface of the culture platform 1, and the balance display module 7 is used to display the horizontal state of the culture platform 1, so as to facilitate the leveling of the culture platform 1.

[0032] The culture platform 1 is provided with a bag fixing structure 9 for fixing the embryo culture bag. Two groups of bag fixing structures 9 are provided in the placement groove 11, respectively located at the two ends of the placement groove 11. The bag fixing structure 9 includes two support columns 91 fixed on the bottom surface of the placement groove 11 and a bag fixing clamp 92 connected between the two support columns 91. One end of the bag fixing clamp 92 is rotatably connected to the top of one support column 91, and the rotation axis is horizontal, so that the bag fixing clamp 92 can swing up and down. The other end of the bag fixing clamp 92 is bolted to another support column 91, and the bolt passes through the through hole at the end of the bag fixing clamp 92 and is threadedly connected to the upper end of the support column 91. In this embodiment, the bag body fixing splint 92 is U-shaped, with both ends tilted upward, and is provided with a connection portion connected to the support column 91, one end of the connection portion is rotatably connected to one support column 91, and the other end of the connection portion is bolted to another support column 91. The bottom of the U-shaped structure of the bag body fixing splint 92 is used to abut the bottom surface of the placement groove 11. In order to protect the embryo culture bag, a flexible protective layer can be provided at the bottom of the U-shaped structure of the bag body fixing splint 92. The embryo culture bag fixing method is not limited to the above, and may also include threaded connection fixation or hinge crimping fixation.

[0033] The motion support structure 2 is connected to the bottom of the culture platform 1, and is used to drive the culture platform 1 to move along the four-axis direction. In this embodiment, the motion support structure 2 is configured as a four-axis motion platform, and the culture platform 1 is installed on the motion table of the motion support structure 2, so that the culture platform 1 can move along the X-axis and the Y-axis, thereby driving the embryo culture bag to perform irregular or regular motion.

[0034] The control host includes a casing body 10, a whole machine switch 3, a control knob 4 and a display screen 5. A control system is arranged inside the casing body 10 for controlling the whole machine. The whole machine switch 3, the control knob 4 and the display screen 5 are arranged on the front side of the casing body 10 and are all connected to the control system. The display screen 5 can be used to view setting-related parameters.

[0035] The embryo culture bag includes a bag body 8 and a ventilation pipeline and a fluid exchange pipeline connected to the bag body 8. The bag body 8 is placed in the placement groove 11 of the culture platform 1. The two ends of the bag body 8 are pressed and fixed by two groups of bag body fixing structures 9. In this embodiment, the bag body 8 is made of a special film for embryo culture. A pipeline mounting seat 81 and a sensor interface 82 are provided on the upper side of the bag body 8. The pipeline mounting seat 81 and the sensor interface 82 are both embedded in the bag body 8. Four pipeline mounting seats 81 are provided for installing ventilation pipelines and fluid exchange pipelines. The sensor interface 82 is used to install a sensor for monitoring the environment inside the bag body 8.

[0036] The ventilation pipeline includes an air inlet pipe 83 and an air outlet pipe 84. The air inlet pipe 83 and the air outlet pipe 84 pass through the pipeline mounting seat 81 and enter the bag body 8. A filter 85 is arranged on the outer end of the air inlet pipe 83 to filter the gas entering the bag body 8. The outer ends of the air inlet pipe 83 and the air outlet pipe 84 are both provided with a cut-off clamp 86 to prevent the air from escaping from the bag body 8. In order to prevent the ventilation pipeline from causing damage to the embryo, the air inlet pipe 83 and the air outlet pipe 84 are arranged in the up-down direction in this embodiment and are located at the edge of the bag body 8. The lower ends of the air inlet pipe 83 and the air outlet pipe 84 are fixed to the lower side of the bag body 8. A positioning plug is fixedly arranged inside the lower side of the bag body 8 to block the lower ports of the air inlet pipe 83 and the air outlet pipe 84 by the positioning plug. Ventilation holes are arranged on the side walls of the air inlet pipe 83 and the air outlet pipe 84 near the pipeline mounting seat 81 to facilitate ventilation in the bag body 8.

[0037] The liquid exchange pipeline includes a liquid inlet pipe 87 and a liquid outlet pipe 88, which pass through the pipeline mounting seat 81 and enter the bag body 8. A filter 85 is provided on the outer end of the liquid inlet pipe 87 to filter the liquid entering the bag body 8. A cut-off clamp 86 is provided on the outer ends of the liquid inlet pipe 87 and the liquid outlet pipe 88 to prevent liquid from running out of the bag body 8. In order to prevent the ventilation pipeline from causing damage to the embryo, the liquid inlet pipe 87 and the liquid outlet pipe 88 are arranged in the up-down direction in this embodiment and are located at the edge of the bag body 8. The lower ends of the liquid inlet pipe 87 and the liquid outlet pipe 88 are fixed to the lower side of the bag body 8. A positioning plug is fixedly provided inside the lower side of the bag body 8, and the lower ports of the liquid inlet pipe 87 and the liquid outlet pipe 88 are blocked by the positioning plug. A liquid through hole is provided on the side wall of the liquid inlet pipe 87 and the liquid outlet pipe 88 near the positioning plug, so as to facilitate liquid exchange in the bag body 8.

[0038] The implementation principle of the present application embodiment is: the input and output ends of the gas source pipeline and the culture solution pipeline are respectively connected to the pipeline corresponding to the embryo culture bag, the input gas sets up the gas environment suitable for embryo culture, and then the input culture solution reaches a certain value, and the sensor interface 82 accesses the corresponding sensor, and the relevant parameters of the motion platform 1 are set, such as temperature, motion angle and speed, etc., and after the embryo is moved into, the embryo starts to move randomly in the embryo culture bag. In order to achieve a constant heat preservation effect, a cover can also be installed on the whole machine platform, and a small window is opened to observe the current situation of the embryo. All parameters of embryo culture can be displayed and derived in real time.

[0039] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A ventilated and liquid-exchangable embryo culture device that simulates the intrauterine environment, characterized in that: The invention comprises a motion culture platform and an embryo culture bag arranged in the motion culture platform. The motion culture platform comprises a culture platform (1), a motion support structure (2) connected below the culture platform (1), and a control host arranged below the motion support structure (2). The control host controls the culture platform (1) to move regularly or irregularly. The embryo culture bag comprises a bag body (8) and a ventilation pipeline and a liquid exchange pipeline connected to the bag body (8). The embryo culture bag is placed in the culture platform (1). A bag body fixing structure (9) for fixing the embryo culture bag is arranged in the culture platform (1).

2. The ventilated and liquid-exchanged embryo culture device simulating the intrauterine environment according to claim 1, characterized in that: The upper surface of the culture platform (1) is provided with a placement groove (11), and the bottom of the placement groove is provided with a heating module (6).

3. The ventilated and liquid-exchanged embryo culture device simulating the intrauterine environment according to claim 1, characterized in that: A balance display module (7) is provided on the upper surface of the culture platform (1).

4. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 1, characterized in that: The motion support structure (2) is configured as a four-axis motion platform, and the culture platform (1) is installed on the motion platform of the motion support structure (2).

5. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 1, characterized in that: The control host comprises a casing body (10) in which a control system is arranged, and a whole machine switch (3), a control knob (4) and a display screen (5) arranged on the casing body (10); the whole machine switch (3), the control knob (4) and the display screen (5) are all connected to the control system.

6. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 2, characterized in that: The bag body fixing structure is provided with two groups in the placement groove (11), and the bag body fixing structure (9) comprises two support columns (91) fixed on the bottom surface of the placement groove (11) and a bag body fixing clamp (92) connected between the two support columns (91), one end of the bag body fixing clamp (92) is rotatably connected to one support column (91), and the other end is bolted to the other support column (91).

7. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 6, characterized in that: The bag body fixing clamp (92) is U-shaped, with connecting parts connected to the support column (91) provided at both ends, and the bottom of the U-shaped structure of the bag body fixing clamp (92) is used to abut the bottom surface of the placement groove (11).

8. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 1, characterized in that: A pipeline mounting seat (81) and a sensor interface (82) are provided on the upper side of the bag body (8), and the ventilation pipeline and the liquid exchange pipeline are connected to the bag body (8) through the pipeline mounting seat (81).

9. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 8, characterized in that: The ventilation pipeline comprises an air inlet pipe (83) and an air outlet pipe (84), a filter (85) is arranged on the air inlet pipe (83), and a shutoff clamp (86) is arranged on both the air inlet pipe (83) and the air outlet pipe (84).

10. The embryo culture device capable of ventilating and exchanging fluids and simulating the intrauterine environment according to claim 8, characterized in that: The liquid exchange pipeline comprises a liquid inlet pipe (87) and a liquid outlet pipe (88), a filter (85) is arranged on the liquid inlet pipe (87), and a shutoff clamp (86) is arranged on both the liquid inlet pipe (87) and the liquid outlet pipe (88).

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