Dewar flask and its outer container, integrated neck tube, assembly method

The integrated neck tube design solves the problem of misalignment of the Dewar bottleneck tube, ensuring stable transportation of biomaterials in non-upright states, simplifying operation and extending retention time.

CN119486947BActive Publication Date: 2025-07-25MVE BIOLOGICAL SOLUTIONS US LLC
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Patent Information

Application Number
CN202380039784.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2022-05-09
Filing Date
2023-05-03
Publication Date
2025-07-25
Estimated Expiration
2043-05-03

AI Technical Summary

Technical Problem

The two-part neck tube design of existing dewar bottles is prone to misalignment during transportation, making it difficult to insert and retrieve biological materials, increase labor and cost, and reduce the time to maintain in an in-upright state, impairing the viability of biological materials.

Method used

The integrated neck tube design is adopted, and by integrating the unique connection between the inner container and the outer container, an integrated neck tube, including the first and second parts, is coupled to the inner and outer containers, respectively, and is fixed by a collar, ensuring concentric alignment and reducing the risk of misalignment.

Benefits of technology

It realizes the stable insertion and retrieval of biological materials in non-upright state, extends the retention time of biological materials, and reduces operational complexity and cost.

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Abstract

A Dewar flask for storing cryogenic fluids includes an inner container, an outer container, and an integral neck tube. The outer container includes an outer upper sheath and an outer lower sheath, configured to be joined and form an inner chamber. The inner container is located within the inner chamber such that a thermal insulation space is defined. The thermal insulation space is at least partially evacuated of air. The integral neck tube extends between a central region of the inner container and the outer upper sheath. The integral neck tube includes a first portion configured to be coupled to the upper outer sheath and a second portion configured to be coupled to the inner container such that the second portion extends into the inner container. The second portion is integrally formed as a single piece with the first portion.
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Description

[0001] Cross - Reference to Related Applications

[0002] This application claims priority to U.S. Provisional Patent Application No. 17 / 739,734, filed on May 9, 2022, entitled "ONE - PIECE NECKTUBE", the entire content of which is incorporated herein by reference. Technical Field

[0003] The present disclosure generally relates to cryogenic fluid containers and, more particularly, to dewars characterized by a one - piece necktube and retainer construction. Background Art

[0004] Containers called dewars are used to store and transport cryogenic materials (e.g., liquid nitrogen boils at 77K or - 196°C at atmospheric pressure). They must have minimal thermal connection between an outer container and an inner container containing the cryogenic material (usually liquid nitrogen, also called LN2, and some valuable items or substances that must be kept cooled by LN2 as it evaporates due to the tiny heat leaking in from the outside into the inner container), including a vacuum - pumped space. The space between the containers is evacuated to eliminate convective heat transfer.

[0005] The only inevitable connection between the inner and outer containers in a dewar is the neck opening to allow filling and emptying of the inner container from the outside. Preferably, these necks made of laminated fiberglass / epoxy resin are the only such connection, such that the inner container and all its contents are exclusively suspended by the neck attached to the top region of the outer container.

[0006] Cryogenic vapor shippers are used for transportation and typically include a cylindrical perforated retainer made of aluminum or stainless steel that forms a barrier between a liquid nitrogen absorbent and the payload area of the inner container of a dewar containing biological materials. The retainer allows access to the biological materials in the payload area of the dewar. The perforations are required to fill the absorbent with liquid nitrogen before transporting biological materials. The liquid nitrogen absorbent prevents spillage in the event of the dewar being accidentally tipped over.

[0007] Accordingly, the two components of the existing two-part neck tube and absorbent retainer must be concentrically aligned to allow insertion and removal of the biological material stored in the payload area of the vapor shipper. If misaligned due to a hard blow during factory production or handling and transportation, it is difficult to insert and retrieve the biological material, and in extreme cases of misalignment, the packaging may be damaged, thereby compromising the viability of the biological material. The two-part design also requires additional labor and cost to procure the two parts and assemble them into the cryogenic vapor shipper. When the cryogenic vapor shipper is in an upright position, the vapor shipper reaches thermal equilibrium and operates optimally. In the reality of the shipping industry, not all vapor shippers transport Dewars in an upright position, and any misaligned orientation will result in a reduced holding time of the Dewar, potentially compromising the biological material in the Dewar. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1A is a cross-sectional side view of a Dewar according to an exemplary embodiment;

[0009] Figure 1B is a top view of a Dewar according to an exemplary embodiment;

[0010] Figure 2A is a schematic view of a one-piece neck tube of the Dewar of FIG. 1 according to an exemplary embodiment;

[0011] Figure 2B is a cross-sectional side view of the Dewar of FIG. 1 according to an exemplary embodiment, showing the relationship between the Dewar and Figure 2A the one-piece neck tube;

[0012] Figure 3A is a detailed view of section A of FIG. 2 according to an exemplary embodiment; and

[0013] Figure 3B is a cross-sectional view of section A of FIG. 2 according to an exemplary embodiment. SUMMARY OF THE INVENTION

[0014] There are several aspects of the present subject matter that can be implemented individually or together in the devices and systems described and claimed below. These aspects can be used alone or in combination with other aspects of the subject matter described herein, and the description of these aspects together is not intended to exclude the use of these aspects alone or the claiming of these aspects alone or in different combinations, as set forth in the appended claims herein. In one aspect, a cryogenic fluid storage dewar includes an inner container, an outer container, and a one-piece neck tube configured to store cryogenic fluid. The outer container includes an outer upper sheath and an outer lower sheath configured to be joined and define an inner chamber. The outer upper sheath includes a central region and a collar. The inner container is positioned within the inner chamber such that a thermal insulation space is defined between the inner container and the outer container. The thermal insulation space is at least partially evacuated of air. The one-piece neck tube extends between the central region of the inner container and the outer upper sheath. The one-piece neck tube includes a first portion and a second portion, the first portion being configured to be coupled to the outer upper sheath, the first portion having a first outer diameter, the second portion being configured to be coupled to the inner container such that the second portion extends into the inner container, the second portion having a second outer diameter. The second portion is integrally formed as a single piece with the first portion.

[0015] In another aspect, a one-piece neck tube of a cryogenic fluid storage dewar includes: a first portion configured to be coupled to an outer container of the dewar, the first portion having a first outer diameter; and a second portion configured to be coupled to an inner container of the dewar, the second portion having a second outer diameter equal to the first outer diameter. The first portion and the second portion are parts of the same piece of fiberglass-reinforced material.

[0016] In another aspect, an outer container of a cryogenic fluid storage dewar includes an outer upper sheath, an outer lower sheath configured to be joined to the outer upper sheath, a one-piece neck tube, and a collar. The outer upper sheath and the outer lower sheath together define an inner chamber. The one-piece neck tube is adapted to be attached to an inner container disposed within the inner chamber of the dewar. The one-piece neck tube includes a first portion coupled to the outer upper sheath and a second portion configured to be coupled to the inner container. The collar is configured to secure the one-piece neck tube to the outer upper sheath.

[0017] In another aspect, a method of assembling a cryogenic fluid storage dewar includes joining an inner upper sheath and an inner lower sheath such that an inner container is formed, the inner container being configured to store biological material, coupling a one-piece neck tube to the inner upper sheath, positioning the inner container within an outer lower sheath, coupling the one-piece neck tube to an outer upper sheath, and joining the outer upper sheath and the outer lower sheath such that an outer container is formed. The outer container is configured to receive the inner container. The inner container and the outer container are joined only by the one-piece neck tube. Detailed Description

[0018] Exemplary embodiments of the present invention are described below.

[0019] Referring Figure 1A and 1B , a Dewar flask 100 according to an exemplary embodiment described herein is shown. The Dewar flask 100 includes an inner container 102 positioned within an outer container 104, with a heat-insulating space 106 formed therebetween.

[0020] The inner container 102 includes an inner upper sheath 108 and an inner lower sheath 110. The inner upper sheath 108 and the inner lower sheath 110 may be welded together to form the inner container 102. The inner upper sheath 108 includes an opening 109. The opening 109 may be provided at a central position of the inner upper sheath 108. The opening 109 may be circular such that the opening 109 is configured to receive a one-piece neck tube as described herein. The opening 109 further includes a flange 111. The flange 111 may axially extend from the opening 109 along the circumference of the opening 109 such that the flange 111 forms a cylindrical inlet. The inner upper sheath 108 may be configured in a generally arcuate shape such that the inner upper sheath 108 extends from the opening 109 to the inner lower sheath 110 with a first downward slope. Thus, the inner upper sheath 108 may have a generally arcuate cross-section such that the inner upper sheath is a dome. The inner lower sheath 100 may be generally cylindrical.

[0021] Similarly, the outer container 104 includes an outer upper sheath 112 and an outer lower sheath 114. The outer upper sheath 112 and the outer lower sheath 114 may be welded together to form the outer container 104. The inner upper sheath 108, the inner lower sheath 110, the outer upper sheath 112, and the outer lower sheath 114 may be made of flat aluminum alloy. The outer upper sheath 112 may include an opening 113. The opening 113 may be provided at a central position along a central region 117 of the outer upper sheath 112. The opening 113 may be circular such that the opening 113 is configured to receive a one-piece neck tube as described herein. For example, the opening 113 receives the same one-piece neck tube received by the opening 109 of the inner upper sheath 108. The opening 113 further includes a flange 115. The flange 115 may axially extend from the opening 113 along the circumference of the opening 113 such that the flange 115 forms a cylindrical inlet. The outer upper sheath 112 may be configured in a generally arcuate shape such that the outer upper sheath 112 extends from the opening 113 to the outer lower sheath 114 with a second downward slope. The second downward slope may be greater than the first downward slope. The outer lower sheath 114 may be generally cylindrical.

[0022] Assemble and weld the inner container 102 configured to store biological materials. The inner container 102 can be wrapped with a multi-layer radiation-reflective material and inserted into a partially complete outer container 104 (e.g., positioned within the outer lower sheath 114). The outer lower sheath 114 can then receive the outer upper sheath 112 welded thereto, such that an inner chamber containing the inner container 102 is formed, the inner chamber including a thermal insulation space 106 between the inner container 102 and the outer container 104.

[0023] The dewar 100 can also include a valve 116. The valve 116 can be disposed on the outer surface of the outer upper sheath 112 (e.g., opposite the inner chamber). The valve 116 can be configured to couple to a vacuum (not shown). Accordingly, the valve is configured to evacuate air from the thermal insulation space 106, thereby providing vacuum insulation within the dewar 100.

[0024] The dewar 100 includes a one-piece neck tube 118. The one-piece neck tube 118 includes a single tube component. The one-piece neck tube is a one-piece dewar neck tube with an integrated retainer, thus preventing cold nitrogen from completely escaping the dewar, for example if the dewar is inverted. The one-piece neck tube 118 is configured to extend between the inner container 102 and the outer container 104. In particular, the one-piece neck tube 118 extends from the inner lower sheath 110 to the outer upper sheath 112. The one-piece neck tube 118 is configured to be received by the opening 109 of the inner container 102 and the opening 113 of the outer container 104. For example, the one-piece neck tube 118 can be attached to the inner upper sheath 108 and project from the inner upper sheath 108 and extend to the outer upper sheath 112. The one-piece neck tube 118 attaches the inner upper sheath 108 to the outer upper sheath 112. In various embodiments, the one-piece neck tube 118 is the only attachment between the inner upper sheath 108 and the outer upper sheath 112. In various embodiments, the one-piece neck tube 118 attaches the inner upper sheath 108 and the outer upper sheath 112, and no other structure attaches the inner upper sheath 108 and the outer upper sheath 112. The one-piece neck tube 118 is configured to allow access to the biological materials stored in the cryogenic dewar. The length of the one-piece neck tube 118 can be approximately 20 inches (e.g., 20.0, 20.5, 20.8, 20.11 inches, including the end values), or a length of approximately 510 millimeters (e.g., 509, 509.5, 510.8 millimeters, including the end values). As needed, the one-piece neck tube 118 can be other lengths. Additionally, the one-piece neck tube 118 can be composed of a composite material. For example, the one-piece neck tube 118 can be composed of fiberglass-reinforced epoxy resin. The one-piece neck tube 118 can be composed of other materials.

[0025] Now refer to Figure 2A 、 2B, 3A and 3B, the integral neck tube 118 includes a first portion 120 and a second portion 122. The first portion 120 is configured to be coupled to the outer container 104. In particular, the first portion 120 is configured to be coupled to the outer upper sheath 112. The first portion 120 has a first end 124, a second end 126, and a first diameter D1. The first diameter D1 is the outer diameter of the first portion 120. The first diameter D1 can be approximately 2.5 inches (e.g., 2.48, 2.5, 2.52 inches, including the end values) or approximately 63.5 millimeters (e.g., 62.9, 64.0 millimeters, including the end values). As needed, the integral neck tube 118 can be of other diameters. The second portion 122 is configured to be coupled to the inner container 102. In particular, the second portion 122 is configured to be coupled to the inner upper sheath 108. In addition, the second portion 122 extends into the inner container 102. The second portion 122 of the integral neck tube 118 can extend through the entire length of the inner container 102. The second portion 122 has a first end 128, a second end 130, and a second diameter D2. The second diameter D2 is the outer diameter of the second portion 130. The second end 124 of the first portion abuts the first end 128 of the second portion 122 and is integrally formed with the first end 128 of the second portion 122 as an integral material. The first portion 120 and the second portion 122 are a continuously integrally formed integral structure. Additionally, the second diameter D2 is equal to the first diameter D1. Thus, the first portion 120 and the second portion 122 define a single neck tube (e.g., the integral neck tube 118).

[0026] The integral neck tube 118 further includes a first sealing section 132 disposed adjacent to the first end 124 of the first portion 120. The first sealing section 132 has a third diameter D3 that is larger than the first diameter D1. The third diameter D3 is the outer diameter of the first sealing section 132. The third diameter D3 can be approximately 2.75 inches (e.g., 2.74, 2.76 inches, including the end values) or about 69.9 millimeters (e.g., 69.7, 69.8, 70.2 millimeters, including the end values). For example, the first sealing section 132 is a thickened portion of the integral neck tube 118. The first sealing section 132 can include an annular thickened portion of the integral neck tube 118. The annular thickened portion can refer to a local increase in the size of the outer diameter of the integral neck tube 118. Thus, the first sealing section 132 is configured to seal the integral neck tube 118 to the outer upper sheath 112. In particular, the first sealing section 132 is configured to be in a facing relationship with the flange 115 of the outer container 104.

[0027] The one-piece neck tube 118 further includes a second seal section 134 disposed along the second end 124 of the first section 120. The second end 124 corresponds to the first end 126 of the second part 122 such that the second seal section 134 is where the first part 120 and the second part 122 meet. The second seal section 134 has a fourth diameter D4 that is larger than the first diameter D1. The fourth diameter D4 is the outer diameter of the second seal section 134. The fourth diameter D4 can be equal to the third diameter D3. The fourth diameter D4 can be less than or greater than the third diameter D3. The second seal section 134 is a thickened portion of the one-piece neck tube 118. The second seal section 134 can include an annular thickened portion of the one-piece neck tube 118. The annular thickened portion can refer to a local increase in the size of the outer diameter of the one-piece neck tube 118. Thus, the second seal section 134 is configured to seal the one-piece neck tube 118 to the inner upper sheath 108. In particular, the second seal section 134 is configured to be in a facing relationship with the flange 111 of the inner container 102.

[0028] The one-piece neck tube 118 further includes a perforated area 136. The perforated area 136 is disposed along at least a portion of the second part 122. The perforated area 136 can include a plurality of perforations 138 (e.g., holes, openings, orifices, etc.) along the surface of the second part 122. For example, the perforated area 136 can include 48 perforations, where the 48 perforations are in an 8×6 configuration. In various embodiments, other suitable amounts and configurations can be included. The second part 122 of the one-piece neck tube 118 forms a barrier between the liquid nitrogen absorbent and the payload area of the dewar containing the biological material. The perforated area 136 allows access to the biological material in the payload area of the dewar. The perforated area 136 allows the absorbent material to be filled with liquid nitrogen prior to transporting the biological material. For example, the location of the plurality of perforations 138 along with the one-piece design will slow the escape of the cryogen in the non-erect position dewar during transportation, extending the holding time and viability of the biological sample inside the canister. In particular, when the dewar is inverted, the inner upper sheath 108 intercepts the cold nitrogen vapor, extending the length of time the payload will remain at cryogenic temperatures. The location of the plurality of perforations 138 along the second part 122 of the one-piece neck tube 118 affects the volume of the intercepted cold nitrogen, where the farther the perforated area 136 is from the collar of the inner upper sheath 108 (e.g., disposed around the flange 111), the colder the vapor that will be intercepted, thus extending the cold temperature holding time. However, if the perforated area 136 is located too far from the collar of the inner upper sheath 108, the canister will not be filled with liquid nitrogen as quickly as when the perforated area 136 is closer to the collar of the inner upper sheath 108.

[0029] Return reference Figure 1A and 1B, the Dewar flask 100 includes a collar 140. The final joint between the integral neck tube 118 and the outer container 104 is the collar 140. The collar 140 is configured to fix the integral neck tube 118 to the outer container 104. For example, when positioned, the first sealing section 132 of the integral neck tube 118 is disposed within the flange 115 of the outer container 104. Thus, the collar 140 is then disposed around the flange 115, completed using an adhesive or other fastening method. For example, the integral neck tube 118 can be coupled to the outer upper sheath 112 via epoxy resin, magnetic forming / crimping, or a combination thereof. Then, the insulating space between the inner container 102 and the outer container 104 is evacuated via the valve 106 to complete the thermal isolation of the inner container.

[0030] The finished Dewar flasks are typically boxed and can be shipped individually or on a pallet to minimize tumbling during shipping. Some cryogenic Dewar flasks will spend their service life under stationary conditions, while other models are designed to travel repeatedly through a shipping handler for transporting cold cryogenic materials. By way of example only, there is liquid nitrogen inside the Dewar flask to keep the transported material cold. Thus, the integral neck tube allows for continuous concentric alignment through such travel / handling to permit insertion and removal of biological materials stored in the payload area of the vapor shipper and can reduce the viability of the biological materials in the event of extreme misalignment.

[0031] Although the preferred embodiments of the present disclosure have been shown and described, it will be apparent to those skilled in the art that changes and modifications can be made therein without departing from the spirit of the present disclosure, and the scope of the present disclosure is defined by the appended claims.

[0032] Benefits, other advantages, and solutions to problems have been described herein with reference to specific embodiments. Additionally, the connecting lines shown in the various figures herein are intended to represent exemplary functional relationships and / or physical couplings between various elements. It should be noted that many alternative or additional functional relationships or physical connections may exist in an actual system. However, these benefits, advantages, solutions to problems, and any element that may cause any benefit, advantage, or solution to occur or become more pronounced should not be construed as a key, essential, or fundamental feature or element of the present disclosure.

[0033] Accordingly, the scope of the present disclosure is limited only by the appended claims, and in the appended claims, unless expressly stated otherwise, the recitation of an element in the singular is not intended to mean "one and only one" but rather "one or more." It should be understood that references to "a," "an," and / or "the" may include one or more than one unless specifically stated otherwise, and references to singular items may also include plural items. All ranges and ratio limitations disclosed herein may be combined.

[0034] In addition, when a phrase such as "at least one of A, B, and C" is used in a claim, it is intended that the phrase be interpreted to mean that A can exist alone in an embodiment, B can exist alone in an embodiment, C can exist alone in an embodiment, or any combination of elements A, B, and C can exist in a single embodiment; for example, A and B, A and C, B and C, or A and B and C. Different cross-hatching is used throughout the figures to represent different components, but not necessarily the same or different materials.

[0035] The steps recited in any method or process description can be executed in any order and need not be limited to the order presented. In addition, any reference to a single item that includes multiple embodiments, and any reference to more than one component or step can include a single embodiment or step. For simplicity and clarity, the elements and steps in the figures are shown and need not be reproduced in any particular order. For example, steps that can be executed simultaneously or in a different order are shown in the figures to help enhance understanding of the embodiments of the present invention.

[0036] Any reference to attachment, fixation, connection, or the like can include permanent, detachable, temporary, partial, complete, and / or any other possible attachment options. In addition, any mention of non-contact (or similar phrases) can also include reduced contact or minimal contact. Surface hatching can be used throughout the figures to represent different parts or regions, but not necessarily the same or different materials. In some cases, reference coordinates may be specific to each figure.

[0037] Systems, methods, and apparatuses are provided herein. In the detailed description herein, references to "one embodiment", "an embodiment", "various embodiments", etc., mean that the embodiment may include a specific feature, structure, or characteristic, but each embodiment may not necessarily include the specific feature, structure, or characteristic. Moreover, such phrases do not necessarily refer to the same embodiment. In addition, when a specific feature, structure, or characteristic is described in connection with an embodiment, it is believed that those skilled in the art know how to implement such features, structures, or characteristics in connection with other embodiments, whether or not explicitly described. After reading the specification, those skilled in the relevant art will understand how to implement the present disclosure in alternative embodiments.

Claims

1. A Dewar flask for storing cryogenic fluids, comprising: An inner container configured to store cryogenic fluids; An outer container including an outer upper sheath and an outer lower sheath, the outer upper sheath including a central region and a collar, the outer upper sheath and the outer lower sheath being configured to be joined and defining an inner chamber, the inner container being positioned within the inner chamber of the outer container such that a thermal insulation space is defined between the inner container and the outer container, wherein the thermal insulation space is at least partially evacuated of air; And A one-piece neck tube extending between a central region of the inner container and the outer upper sheath, the one-piece neck tube including: A first portion configured to be coupled to the outer upper sheath, the first portion having a first outer diameter; and A second portion configured to be coupled to the inner container such that the second portion extends into the inner container, the second portion having a second outer diameter, the second portion being integrally formed as a single piece with the first portion, Wherein the one-piece neck includes a first sealing section disposed at a first end of the first portion, the first sealing section having a third outer diameter greater than the first outer diameter such that the first sealing section is configured to seal the one-piece neck tube with the outer upper sheath.

2. The Dewar flask according to claim 1, wherein The second portion includes a perforated region.

3. The Dewar flask according to claim 1, characterized in that, The inner container includes an inner upper sheath and an inner lower sheath joined together.

4. The Dewar flask according to claim 3, wherein, The one-piece neck tube is configured to extend from the inner lower sheath to the outer upper sheath.

5. The Dewar flask according to claim 3, wherein, The one-piece neck tube further includes a second sealing section disposed at a second end of the first portion opposite the first end, the second end corresponding to a first end of the second portion, the second sealing section having a fourth outer diameter greater than the first outer diameter such that the second sealing section is configured to seal the one-piece neck tube with the inner upper sheath.

6. The Dewar flask according to claim 1, characterized in that, The first outer diameter and the second outer diameter are equal.

7. The Dewar flask according to claim 1, wherein, The neck tube is made of glass fiber-reinforced epoxy resin.

8. The Dewar flask according to claim 1, characterized in that, The outer container is made of aluminum alloy.

9. A one-piece neck tube for a Dewar flask for storing cryogenic fluids, the one-piece neck tube including: A first portion configured to be coupled to an outer container of the Dewar flask, the first portion having a first outer diameter; And A second portion configured to be coupled to an inner container of the Dewar flask, the second portion having a second outer diameter equal to the first outer diameter; Wherein the first portion and the second portion are portions of the same piece of glass fiber-reinforced material, and A first sealing section is disposed at a first end of the first portion, the first sealing section having a third outer diameter greater than the first outer diameter such that the first sealing section is configured to seal the one-piece neck tube with the outer container.

10. The integrated cervical canal according to claim 9, characterized in that, The second portion includes a perforated region.

11. The integrated cervical canal according to claim 9, wherein, The one-piece neck tube is configured to extend from the bottom of the inner container to the top of the outer container.

12. The integrated cervical canal according to claim 9, characterized in that, The integrated neck tube further includes a second sealing section disposed at a second end of the first part opposite the first end, the second end corresponding to a first end of the second part, the second sealing section having a fourth outer diameter greater than the first outer diameter such that the second sealing section is configured to seal the integrated neck tube with the inner container.

13. The integrated cervical canal according to claim 9, characterized in that, The first part and the second part are configured to provide a mechanical connection only between the outer container of the Dewar flask and the inner container of the Dewar flask inside the outer container of the Dewar flask to support the inner container suspended inside the insulating space of the Dewar flask.

14. An outer container of a Dewar flask for storing cryogenic fluids, comprising: An outer upper sheath; An outer lower sheath configured to be coupled to the outer upper sheath, the outer upper sheath and the outer lower sheath together defining an inner chamber; An integrated neck tube adapted to be attached to an inner container disposed within the inner chamber of the Dewar flask, the integrated neck tube including: A first part configured to be coupled to the outer upper sheath; and A second part configured to be coupled to the inner container, wherein the second part includes a perforated area, wherein the perforated area is configured to be disposed within the inner container; and A collar configured to fix the integrated neck tube to the outer upper sheath.

15. The outer container according to claim 14, characterized in that, The first part includes a first outer diameter and the second part includes a second outer diameter equal to the first outer diameter, the first outer diameter corresponding to an opening of the outer container and the second outer diameter corresponding to an opening of the inner container.

16. The external container according to claim 15, wherein, The collar is configured to surround the opening of the outer container.

17. A method of assembling a Dewar flask for storing cryogenic fluids, the method comprising: Coupling an inner upper sheath and an inner lower sheath such that an inner container is formed, the inner container being configured to store biological materials; Coupling an integrated neck tube to the inner upper sheath; Positioning the inner container within the outer lower sheath; Coupling the integrated neck tube to the outer upper sheath; Coupling the outer upper sheath and the outer lower sheath such that an outer container is formed, the outer container being configured to receive the inner container, wherein the inner container and the outer container are coupled only through the integrated neck tube; Coupling a first part of the integrated neck tube to the outer upper sheath, the first part having a first outer diameter; Coupling a second part of the integrated neck tube to the inner container such that the second part extends into the inner container, the second part having a second outer diameter, the second part being integrally formed with the first part as a single unit; Sealing the integrated neck tube with the outer upper sheath at a first sealing section provided at a first end of the first part; And Sealing the integrated neck tube with the inner upper sheath at a second sealing section provided at a second end of the first part opposite the first end, the second end corresponding to a first end of the second part, the second sealing section having a fourth outer diameter greater than the first outer diameter.

Citation Information

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