Portable microbial sample collection device and microbial sample collection method

Abstract

The present invention discloses a portable micro-biological sample collection device and a method for collecting micro-organisms, which relate to the technical field of biological sampling. The present invention comprises an external shell, including a placement box, a top shell and a storage box. The placement box has an open top and an installation space inside. The top shell is fixed to the top of the placement box, and the storage box is installed on the top of the top shell; a collection component comprises a pump component, a control module, a power supply module and a collection head. The pump component and the power supply module are both installed in the installation space and connected to each other; the control module is installed on the top shell and electrically connected to the pump component. The collection head is installed outside the placement box and communicated with the pump component. The present invention solves the problems of existing sampling devices such as limited use environment, complex operation, and single function.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sampling, and in particular to a portable micro-biological sample collection device and a micro-organism collection method. Background Art

[0002] At present, in many fields such as outdoor environmental monitoring, field sample collection and laboratory bioassay analysis, artificial screening and collection of micro-samples (such as micro-organism insects, tiny seeds, pollen, etc.) is a key step in experiments and data analysis.

[0003] The existing sampling technologies and devices mainly have the following problems and shortcomings: 1. These devices are often suitable for placement in specific locations in laboratory environments, but are not suitable for use in the field or other unstable environments; 2. Traditional sampling devices are complicated to operate, requiring users to have high technical capabilities, and are not suitable for frequent outdoor use. The sampling process usually requires multiple steps and takes a long time; 3. Some existing sampling devices rely on suction to suck insects into the sampling tube through vacuum. They have no adjustment capabilities and are highly targeted. Their functions are often dedicated to capturing specific types of samples and cannot cope with application scenarios that require collecting multiple types of tiny samples; 4. In existing sampling methods, especially when dealing with tiny samples, the collection process is prone to sample contamination or loss due to interference from the external environment, structural problems with the equipment itself, or improper operation. Summary of the Invention

[0004] In view of the problems existing in the above-mentioned existing sampling devices, such as limited use environment, complex operation, and single function, the present invention is proposed.

[0005] Therefore, the purpose of the present invention is to provide a portable micro-biological sample collection device that can efficiently collect tiny samples in multiple scenarios such as the field and the laboratory, ensure sample integrity, and simplify operation.

[0006] To solve the above technical problems, the present invention provides the following technical solutions: a portable micro-biological sample collection device, comprising an external shell, including a placement box, a top shell and a storage box, the placement box having an open top and an installation space inside, the top shell being fixed to the top of the placement box, and the storage box being installed on the top of the top shell; a collection component, comprising a pump component, a control module, a power supply module and a collection head, the pump component and the power supply module being both installed in the installation space and connected to each other; the control module being installed on the top shell and electrically connected to the pump component, the collection head being installed outside the placement box and communicating with the pump component.

[0007] As a preferred embodiment of the portable micro-biological sample collection device of the present invention, a shoulder strap is connected to the outer wall of the placement box, and ventilation holes and air exchange holes are respectively opened on the opposite side walls; a three-way valve is installed at the position corresponding to the ventilation hole, and a group of connectors of the three-way valve is connected to the ventilation hole.

[0008] As a preferred solution of the portable micro-biological sample collection device of the present invention, a safety lock is fixed to the outer wall of one side of the placement box, and the safety lock includes a toggle buckle and a lock ring rotatably connected to its side wall; an embedded groove is opened at the top of the top shell, and corresponding snap-fit ​​grooves are opened on the inner walls on both sides opposite to the embedded groove.

[0009] As a preferred embodiment of the portable micro-biological sample collection device of the present invention, the collection head includes a ventilation tube, a living body collection head, and a pollen collection head. The living body collection head and the pollen collection head are replaceably installed at one end of the ventilation tube, and the other end of the ventilation tube is movably sealed and inserted into the ventilation hole of the placement box.

[0010] As a preferred embodiment of the portable micro-biological sample collection device of the present invention, the living body collection head includes a first hollow tube and a first contact head connected to the first connector at both ends thereof, a first gauze is fixed at the connection point between the first hollow tube and the first connector, and an inclined surface is provided at the end of the first contact head; the pollen collection head includes a second hollow tube and a second contact head and a second connector connected at both ends thereof, a second gauze is fixed at the connection point between the second hollow tube and the second connector; the inner diameter of the first hollow tube is larger than the inner diameter of the second hollow tube, the axial length of the first hollow tube is smaller than the axial length of the second hollow tube; the axial length of the second contact head is larger than the axial length of the first contact head.

[0011] As a preferred solution of the portable micro-biological sample collection device of the present invention, wherein: the storage box has a storage space, and its outer wall is provided with a perforation, and a third gauze is sealed and fixed in the perforation; the bottom of the outer wall of one side of the storage box is also fixedly connected to a buckle, and the locking ring can be engaged with the buckle; the bottom of the outer walls of the storage box on both sides adjacent to the buckle are symmetrically fixed with card strips, the storage box can be placed in the embedded groove, and the card strip is slidably inserted into the card groove; the top of the storage box is also sealed and fixed with a cover shell, and the top of the cover shell is also fixed with a handle and a storage hole, and the storage hole is connected to the storage space.

[0012] As a preferred embodiment of the portable micro-biological sample collection device of the present invention, the pump assembly includes a symmetrically arranged air pump, which includes a driving part and a working part fixed at one end thereof, and the driving part is fixed to the bottom wall of the placement box through a base; an air supply pipe is also connected to the outside of the symmetrical working part, and the air supply pipe is connected to the remaining two sets of connectors of the three-way valve.

[0013] As a preferred embodiment of the portable micro-biological sample collection device of the present invention, the control module includes symmetrically arranged control panels, which are mounted on the top shell. A switch is fixed between the control panels, and the switch controls the power on and off of the control panels on both sides. The control panel is electrically connected to the air pump and controls the start and stop of the air pump.

[0014] As a preferred solution of the portable micro-biological sample collection device of the present invention, the power supply module includes a power supply and a voltage regulator electrically connected thereto, and the voltage regulator is electrically connected to the air pump and the switch.

[0015] In order to solve the above technical problems, the present invention also provides the following technical solutions: a method for collecting microorganisms, which is based on the above-mentioned portable micro-biological sample collection device, including: first selecting the type of sample to be collected; if the sample type is pollen, inserting the second contact head into the center of the flower, adjusting the suction of the air pump, and continuously sucking the pollen into the second hollow tube for collection, and then switching to control another air pump through the control module to discharge and collect the pollen in the second hollow tube; if the sample type is a living organism, the inclined surface of the first contact head is abutted against the surface of the plant leaf to ensure that the sample is located within the aperture of the first contact head, and then adjusting the suction of the air pump, and continuously sucking the living organism into the first hollow tube for collection, and then switching to control another air pump through the control module to discharge the living organism in the first hollow tube into the storage box.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. Easy to carry and use in multiple scenarios: The design is compact and lightweight, and can be carried by a crossbody strap, suitable for use in the field and laboratory.

[0018] 2. The sampling force is adjustable to avoid sample damage: the sampling head can adjust the sampling force at any time through the voltage adjustment knob, which is suitable for a variety of sample types.

[0019] 3. Modular design, easy replacement of sampling heads: users can freely replace sampling heads according to the type of samples without complicated operations, reducing damage to the original environment of the samples.

[0020] 4. Long-lasting battery, suitable for long-term use in the field: rechargeable lithium battery pack provides long-term battery life.

[0021] 5. Breathable and sealed storage options: ensure that samples will not be affected by moisture or mildew, effectively avoiding contamination or loss. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them:

[0023] Figure 1 This is a schematic diagram of the overall structure of the portable micro-biological sample collection device of the present invention.

[0024] Figure 2 This is a schematic diagram of the internal structure of the placement box of the portable micro-biological sample collection device of the present invention.

[0025] Figure 3 This is a schematic diagram of the external structure of the portable micro-biological sample collection device of the present invention.

[0026] Figure 4 This is a schematic structural diagram of the collection head of the portable micro-biological sample collection device of the present invention.

[0027] Figure 5 This is a flowchart of the use of the portable micro-biological sample collection device of the present invention. DETAILED DESCRIPTION

[0028] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0029] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0031] Furthermore, the present invention is described in detail with reference to schematic diagrams. For ease of illustration, when describing the embodiments of the present invention, cross-sectional views illustrating device structures may be partially enlarged and not to scale. Furthermore, the schematic diagrams are merely illustrative and should not limit the scope of protection of the present invention. Furthermore, in actual production, the three-dimensional dimensions of length, width, and depth should be included.

[0032] Example 1

[0033] Reference Figures 1 to 4 , which is the first embodiment of the present invention, provides a portable micro-biological sample collection device, which includes an external shell 100, including a placement box 101, a top shell 102 and a storage box 103. The placement box 101 has an open top and has an installation space A inside. The top shell 102 is fixed on the top of the placement box 101, and the storage box 103 is installed on the top of the top shell 102.

[0034] The placement box 101 is made of aluminum or plastic, and is preferably a rectangular box structure with an opening on the top. The internal installation space A is used to install and place the collection component 200.

[0035] Furthermore, the top shell 102 is a rectangular plate structure, which can be fitted over the top opening of the storage box 101 to protect the internal components. The storage box 103 is slidably connected to the top of the top shell 102 and can be flexibly assembled and disassembled.

[0036] Furthermore, the interior of the storage box 103 can simulate a natural environment by placing some plants or sand and gravel, which can increase the survival time when storing living samples.

[0037] The collection assembly 200 includes a pump assembly 201 , a control module 202 , a power supply module 203 and a collection head 204 . The pump assembly 201 and the power supply module 203 are both installed in the installation space A and connected to each other.

[0038] The control module 202 is installed on the top shell 102 and is electrically connected to the pump assembly 201 . The collection head 204 is installed outside the placement box 101 and is in communication with the pump assembly 201 .

[0039] Among them, the pump component 201 plays the role of suction and blowing, and cooperates with the collection head 204 to absorb samples. The power supply module 203 preferably adopts a rechargeable power supply. This module provides power for the device and is suitable for environments without power supply. It contains a set of 6800mA rechargeable batteries, whose power can support long-term sampling operations and the battery life can reach more than 12 hours.

[0040] Furthermore, the power supply module 203 is provided with a DC charging port, which can power the device and charge the battery at the same time.

[0041] The outer wall of the storage box 101 is connected to a shoulder strap 101a, and its opposite side walls are respectively provided with a vent hole 101b and a ventilation hole 101c; a three-way valve 101b-1 is installed at the corresponding position of the vent hole 101b, and a group of connectors of the three-way valve 101b-1 are connected to the vent hole 101b.

[0042] Furthermore, the shoulder strap 101a is used to carry the device diagonally in the field, thereby freeing both hands to operate sampling; the three-way valve 101b-1 preferably adopts an electromagnetic three-way air valve with an input voltage of DC 6V, which is used to change the airflow direction in the trachea and the sampling head.

[0043] A safety lock buckle 101d is fixed to the outer wall of one side of the storage box 101. The safety lock buckle 101d includes a toggle buckle 101d-1 and a locking ring 101d-2 rotatably connected to its side wall. When the toggle buckle 101d-1 is moved, the locking ring 101d-2 will lock the storage box 103; an embedded groove 102a is opened at the top of the top shell 102, and the inner walls on both sides opposite to the embedded groove 102a are correspondingly opened with snap-fit ​​grooves 102a-1.

[0044] The collecting head 204 includes a vent tube 204a, a living body collecting head 204b and a pollen collecting head 204c. The living body collecting head 204b and the pollen collecting head 204c are replaceably installed at one end of the vent tube 204a. The other end of the vent tube 204a is movably sealed and plugged into the vent hole 101b of the placement box 101.

[0045] The ventilation tube 204a is preferably a silicone hose, which is used to connect the collection head 204 and the pump assembly 201.

[0046] The biopsy collection head 204b includes a first hollow tube 204b-1 and first contact heads 204b-2 and a first connector 204b-3 connected at both ends thereof. A first gauze 204b-4 is fixed to the first hollow tube 204b-1 at the connection point with the first connector 204b-3. An inclined surface X is formed at the end of the first contact head 204b-2.

[0047] The pollen collecting head 204c includes a second hollow tube 204c-1 and a second contact head 204c-2 and a second connector 204c-3 connected at both ends thereof. A second gauze 204c-4 is fixed at the connection between the second hollow tube 204c-1 and the second connector 204c-3.

[0048] The inner diameter of the first hollow tube 204b-1 is larger than that of the second hollow tube 204c-1, and the axial length of the first hollow tube 204b-1 is smaller than that of the second hollow tube 204c-1; the axial length of the second contact head 204c-2 is larger than that of the first contact head 204b-2.

[0049] Among them, the living collection head 204b is used to collect smaller insects, such as large thrips, ants or fruit flies. The first hollow tube 204b-1 adopts a 1.5ml centrifuge tube, and the first gauze 204b-4 can adopt a ball-shaped or plate-shaped gauze, preferably with a gauze density of 200 mesh to prevent the escape and damage of tiny insects; the inclined surface X at the end of the first contact head 204b-2 can make it easier to fit on leaves or flower petals to collect insects on the leaves.

[0050] Furthermore, the second hollow tube 204c-1 is preferably made of a 5ml pipette tip, and is top-encapsulated with two layers of 300-mesh second gauze 204c-4 to prevent pollen leakage, and is suitable for collecting fine particle samples.

[0051] Furthermore, the pollen collecting head 204c is long and thin and can be directly inserted into the center of various flowers. Since the pistil is generally located at the innermost part, a longer pollen tube is more convenient and the pollen tube does not need to worry about insect death, so more pollen can be collected.

[0052] Furthermore, insects such as thrips usually crawl inside the flowers. The inclined X-side opening will not directly suck the bottom of the flower and damage the center of the flower. Large insects crawl on the beans or petals of the plant. This is done to suit most situations. Sometimes there will be dew in the center of the flower, and once the water is sucked in, the insects will be drowned.

[0053] Example 2

[0054] Reference Figures 1 to 4 , which is the second embodiment of the present invention. This embodiment is different from the first embodiment in that: a storage box 103 has a storage space B, and a through-hole 103a is opened on its outer wall. A third gauze 103b is sealed and fixed in the through-hole 103a.

[0055] The perforations 103a are preferably provided in four groups, two of which are opposite to each other. The third gauze 103b is made of 300-mesh metal gauze, which is suitable for living samples that require ventilation to prevent the samples from becoming moldy or rotting during storage.

[0056] Furthermore, the storage space B is used to store live insects. When the sample collection volume is large and the sample activity needs to be maintained, the simulated ecological environment in the storage space B can achieve this purpose.

[0057] A buckle 103c is also fixedly connected to the bottom of the outer wall of one side of the storage box 103, and the locking ring 101d-2 can be fitted into the buckle 103c.

[0058] The storage box 103 is symmetrically fixed with clips 103d on the bottom of the outer walls on both sides adjacent to the buckle 103c. The storage box 103 can be placed in the embedded groove 102a, and the clips 103d are slidably inserted into the clip groove 102a-1.

[0059] During use, the storage box 103 can be slid into the embedded groove 102a, and the storage box 103 can be finally locked through the cooperation between the buckle 103c and the locking ring 101d-2.

[0060] A cover shell 103e is also sealed and fixed on the top of the storage box 103. A handle 103e-1 and a storage hole 103e-2 are also fixed on the top of the cover shell 103e. The storage hole 103e-2 is connected to the storage space B.

[0061] By inserting the first contact head 204b-2 into the storage hole 103e-2, the insects temporarily sucked into the first hollow tube 204b-1 can be discharged into the storage space B.

[0062] The pump assembly 201 includes a symmetrically arranged air pump 201a, which includes a driving part 201a-1 and a working part 201a-2 fixed at one end thereof. The driving part 201a-1 is fixed to the bottom wall of the placement box 101 through the base 201a-1a. The driving part 201a-1 has a built-in motor, and the rotor in the working part 201a-2 rotates to form a vacuum environment.

[0063] The air pump 201a is preferably a 555 electric air pump, and one of the two air pumps 201a is an air suction pump and the other is an air blowing pump.

[0064] The symmetrical working portion 201a-2 is further connected to an air delivery pipe 201a-2a, which is connected to the remaining two sets of connectors of the three-way valve 101b-1.

[0065] The control module 202 includes a symmetrically arranged control panel 202a, which is installed on the top shell 102. A switch 202b is also fixed between the control panels 202a. The switch 202b controls the power on and off of the control panels 202a on both sides; the control panel 202a is electrically connected to the air pump 201a and controls the start and stop of the air pump 201a.

[0066] Among them, the switch 202b is a KCD1 three-pin three-speed rocker switch, which is used to switch the power output of the power supply and change the direction of the air flow inside the sampling tube by running different air pumps.

[0067] The power supply module 203 includes a power supply 203a and a voltage regulator 203b electrically connected thereto. The voltage regulator 203b is electrically connected to the air pump 201a and the switch 202b. The voltage regulator 203b can ensure that the device can output stably under different power conditions, is suitable for long-term sampling in harsh environments, and is used to stably convert the input voltage into a 6V output voltage to drive the electromagnetic three-way valve.

[0068] The control panel 202a includes two modules, one for the suction pump power control module and the other for the blowing pump power control module, which can provide 0-12V voltage output for the suction pump and the blowing pump according to sampling requirements.

[0069] Furthermore, the control panel 202a includes a display screen, and the LCD screen displays the current voltage and current of the air pump, so that the user can accurately adjust the air pump output during the sampling process to meet the sampling requirements of different samples.

[0070] The remaining structures are the same as those of Example 1.

[0071] Example 3

[0072] Reference Figures 1 to 5 , which is the third embodiment of the present invention, differs from the second embodiment in that it provides a method for collecting micro-organisms, which is based on the portable micro-biological sample collection device in the above embodiment and includes the following steps:

[0073] S1: First, select the type of sample to be collected.

[0074] S2: If the sample type is pollen, insert the second contact head 204c-2 into the center of the flower, adjust the suction of the air pump 201a, and continuously suck the pollen into the second hollow tube 204c-1 for collection. Then, switch and control another air pump 201a through the control module 202 to discharge and collect the pollen in the second hollow tube 204c-1.

[0075] When collecting pollen grains from crops, there is no need to worry about damaging the sample. The user selects the 5ml pollen collection head 204c and adjusts the sampling voltage to the low flow mode to ensure that the suction force is suitable for pollen collection.

[0076] The sampling head gradually draws pollen into a temporary storage chamber before transferring it to a sealed storage chamber to protect the sample from moisture and contamination. This method allows for efficient collection of pollen grains for botanical research and pollen viability analysis.

[0077] S3: If the sample type is a living organism, the inclined surface X of the first contact head 204b-2 is placed against the surface of the plant leaf to ensure that the sample is within the aperture of the first contact head 204b-2. The suction force of the air pump 201a is then adjusted to continuously suck the living organism into the first hollow tube 204b-1 for collection. The control module 202 then switches to control another air pump 201a to discharge the living organism in the first hollow tube 204b-1 into the storage box 103.

[0078] In order to verify the effect of the suction force of the living body collection head 204b on insects under different output voltages, the following two sets of experiments were conducted:

[0079] 1. Test plan for common giant thrips: Under laboratory conditions, common giant thrips were reared with cowpea segments inside a quartz glass tube with a height of 50 cm, an outer diameter of 38 mm, and an inner diameter of 35 mm. The bottom of the quartz tube was sealed with rice paper and the top was sealed with an ultra-thin transparent sealing film.

[0080] The device was used to collect 60 common giant thrips on cowpea segments each time. During the collection process, the number of test insects was ensured to be sufficient. Different voltages were used for testing, and the time required to collect 60 insects was calculated. The collected 60 insects were placed in a new glass tube, and the insect damage rate was observed and calculated after 1 hour.

[0081] Table 1: Comparison of damage to common giant thrips caused by different voltage outputs

[0082]

[0083] From the above experiments, it can be seen that when the output voltage is 7V, the collection efficiency of the device is the best and the integrity of the sample can be guaranteed.

[0084] 2. Spotted-wing Drosophila Assay Protocol: Under laboratory conditions, spotted-wing Drosophila were raised on an artificial diet in transparent plastic fruit fly bottles sealed with sponge stoppers. The sampling head of the device was placed into the fruit fly bottle by squeezing the sponge stopper. Fifty fruit flies were collected at a time. During the collection process, a sufficient number of flies were ensured in the test bottle. Different voltages were used for testing, and the time required to collect 60 flies was calculated. The collected 60 flies were then placed in a new fruit fly bottle. After 1 hour, the insects were observed and the damage rate was calculated.

[0085] Table 2: Comparison of damage to Drosophila spp. caused by different voltage outputs

[0086]

[0087] From the above experiments, it can be seen that when the output voltage is 6V, the collection efficiency of the device is the best and the integrity of the sample can be guaranteed.

[0088] Furthermore, the body length of an adult common giant thrips is 1.6 mm, while the body length of an adult fruit fly is generally 3 to 4 mm. From the above two groups of experiments, it can be seen that the larger the individual sample size, the more difficult it is to collect. If a larger output power is used, it will cause greater damage to the sample.

[0089] Furthermore, this embodiment preferably uses a 6V output voltage to collect living samples, which can ensure sample integrity while improving collection efficiency.

[0090] The remaining structures are the same as those of Example 2.

[0091] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A portable micro-biological sample collection device, characterized by: include, The outer shell (100) comprises a placement box (101), a top shell (102) and a storage box (103); the placement box (101) is open at the top and has an installation space (A) inside; the top shell (102) is fixed on the top of the placement box (101); and the storage box (103) is installed on the top of the top shell (102); A collection assembly (200) comprises a pump assembly (201), a control module (202), a power supply module (203), and a collection head (204), wherein the pump assembly (201) and the power supply module (203) are both installed in the installation space (A) and are connected to each other; The control module (202) is mounted on the top shell (102) and is electrically connected to the pump assembly (201); the collection head (204) is mounted outside the placement box (101) and is in communication with the pump assembly (201); The outer wall of the storage box (101) is connected to a shoulder strap (101a), and two opposite side walls thereof are respectively provided with a vent hole (101b) and a ventilation hole (101c); A three-way valve (101b-1) is installed at a position corresponding to the vent hole (101b), and a group of connectors of the three-way valve (101b-1) are in communication with the vent hole (101b); A safety lock buckle (101d) is fixed to an outer wall of one side of the storage box (101), and the safety lock buckle (101d) comprises a toggle buckle (101d-1) and a lock ring (101d-2) rotatably connected to the side wall thereof; An embedded groove (102a) is provided on the top of the top shell (102), and corresponding clamping grooves (102a-1) are provided on the inner walls on two opposite sides of the embedded groove (102a); The collection head (204) comprises a ventilation tube (204a), a living body collection head (204b) and a pollen collection head (204c); the living body collection head (204b) and the pollen collection head (204c) are replaceably mounted on one end of the ventilation tube (204a); the other end of the ventilation tube (204a) is movably sealed and plugged into the ventilation hole (101b) of the placement box (101); The living body collection head (204b) comprises a first hollow tube (204b-1) and first contact heads (204b-2) connected at both ends thereof and connected to a first connector (204b-3); a first gauze (204b-4) is fixed in the first hollow tube (204b-1) at a point in communication with the first connector (204b-3); and an inclined surface (X) is provided at the end of the first contact head (204b-2); The pollen collecting head (204c) comprises a second hollow tube (204c-1) and a second contact head (204c-2) and a second connector (204c-3) connected at both ends thereof; a second gauze (204c-4) is fixed at a point in communication between the second hollow tube (204c-1) and the second connector (204c-3); The inner diameter of the first hollow tube (204b-1) is greater than the inner diameter of the second hollow tube (204c-1), and the axial length of the first hollow tube (204b-1) is less than the axial length of the second hollow tube (204c-1); The axial length of the second contact head (204c-2) is greater than the axial length of the first contact head (204b-2).

2. The portable micro-biological sample collection device according to claim 1, characterized in that: The storage box (103) has a storage space (B) therein, and a perforation (103a) is provided on its outer wall, wherein a third gauze (103b) is fixed in a sealing manner in the perforation (103a); A buckle (103c) is also fixedly connected to the bottom of the outer wall of one side of the storage box (103), and the locking ring (101d-2) can be fitted and snapped into the buckle (103c); Clamping strips (103d) are symmetrically fixed to the bottom of the outer walls of both sides of the storage box (103) adjacent to the buckle (103c); the storage box (103) can be placed in the embedded groove (102a); and the clamping strip (103d) is slidably inserted into the clamping groove (102a-1); A cover shell (103e) is also sealed and fixed on the top of the storage box (103), and a handle (103e-1) and a storage hole (103e-2) are also fixed on the top of the cover shell (103e), and the storage hole (103e-2) is communicated with the storage space (B).

3. The portable micro-biological sample collection device according to claim 2, characterized in that: The pump assembly (201) comprises a symmetrically arranged air pump (201a), the air pump (201a) comprising a driving portion (201a-1) and a working portion (201a-2) fixed at one end thereof, the driving portion (201a-1) being fixed to the bottom wall of the placement box (101) via a base (201a-1a); The symmetrical working portion (201a-2) is further connected to an air delivery pipe (201a-2a), and the air delivery pipe (201a-2a) is connected to the remaining two groups of connectors of the three-way valve (101b-1).

4. The portable micro-biological sample collection device according to claim 3, characterized in that: The control module (202) comprises symmetrically arranged control panels (202a), the control panels (202a) being mounted on the top shell (102), and switches (202b) being fixed between the control panels (202a), the switches (202b) controlling the power on and off of the control panels (202a) on both sides; The control panel (202a) is electrically connected to the air pump (201a) and controls the start and stop of the air pump (201a).

5. The portable micro-biological sample collection device according to claim 4, characterized in that: The power supply module (203) comprises a power supply (203a) and a voltage stabilizer (203b) electrically connected thereto, wherein the voltage stabilizer (203b) is electrically connected to the air pump (201a) and the switch (202b).

6. A method for collecting microorganisms, characterized by: Based on the portable micro-biological sample collection device as claimed in claim 5, include, First, select the type of sample you want to collect; If the sample type is pollen, the second contact head (204c-2) is inserted into the center of the flower, the suction force of the air pump (201a) is adjusted, and the pollen is continuously sucked into the second hollow tube (204c-1) for collection, and then another air pump (201a) is switched and controlled by the control module (202) to discharge and collect the pollen in the second hollow tube (204c-1); If the sample type is a living organism, the inclined surface (X) of the first contact head (204b-2) is brought into contact with the surface of a plant leaf to ensure that the sample is located within the aperture of the first contact head (204b-2). The suction force of the air pump (201a) is then adjusted to continuously draw the living organism into the first hollow tube (204b-1) for collection. The control module (202) then switches to control another air pump (201a) to discharge the living organism in the first hollow tube (204b-1) into the storage box (103).

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