Pesticide composition for preventing and treating wheat sharp eyespot and bactericide thereof

Abstract

The invention provides a pesticide composition for preventing and treating wheat sharp eyespot and a bactericide thereof, and discloses an antifungal application method, and belongs to the technical field of plant bactericides, the composition comprises an active component A and an active component B. The active component A is a compound with a structural formula (I), and the active component B is a compound with a structural formula (II). And the active component A is at least one selected from epoxiconazole, hexaconazole, metconazole and prothioconazole, the active component B is at least one selected from epoxiconazole, hexaconazole, metconazole and prothioconazole, the weight ratio of epoxiconazole to hexaconazole to metconazole to prothioconazole is (1.5-20): 1, and through synergistic interaction, the pesticide composition shows a remarkable prevention and treatment effect on wheat sharp eyespot.

Description

Technical Field

[0001] The invention belongs to the field of plant fungicides, and in particular relates to a pesticide composition for preventing and treating wheat sheath blight and a fungicide thereof. Background Art

[0002] Chemical fungicide is the most economical and effective means of plant disease prevention and control. However, due to the single site of action of chemical fungicides of a single variety or a single mode of action, pathogens are prone to develop resistance to such agents.

[0003] Triazole fungicides, such as tebuconazole, fenpropimorph, and fluphenazine, have the characteristics of broad fungicide spectrum, high activity, low cost, and easy use. However, the use of high doses of a single agent will inhibit seed germination and the growth of plant stems and leaves. Once used improperly, it is easy to cause phytotoxicity, affecting the growth and development of plants and even causing plant death.

[0004] Pesticide compounding provides an economical and practical solution to overcome the functional deficiencies of traditional pesticide formulations. Compound formulations consisting of two or more pesticides can exert synergistic effects by utilizing the complementary mechanisms and modes of action of different compounds. These formulations can not only enhance the efficacy of biological control and delay pest resistance, but also extend the shelf life of products and protect ecological diversity.

[0005] However, not every ingredient can be compounded with each other. When different pesticide ingredients are compounded, three types of effects will be exhibited: additive effect, synergistic effect and antagonistic effect.

[0006] Therefore, reasonable pesticide mixing has become one of the important ways to develop new pesticide products. Although scientists know that compounding technology has many advantages, it is a challenging task to select appropriate drugs for reasonable compounding to achieve synergistic effects. Summary of the invention

[0007] The embodiments of the present invention provide a pesticide composition and a fungicide for preventing and controlling wheat stripe blight, which solves the problem that the use of a single agent in high doses will inhibit seed germination and plant stem and leaf growth, and once used improperly, it is easy to cause pesticide damage, affect plant growth and development, and even cause plant death.

[0008] In view of the above problems, the technical solution proposed by the present invention is:

[0009] The present invention provides a pesticide composition for preventing and controlling wheat sheath blight, comprising an active component A and an active component B contained in the composition; the active component A is a compound having a structure shown in formula (I),

[0010]

[0011] The active component B is selected from at least one of epoxiconazole, hexaconazole, metconazole and prothioconazole;

[0012] The weight ratio of the active component A to the active component B is 1.5-20:1.

[0013] As a preferred technical solution of the present invention, the active component B is one or more of epoxiconazole and metconazole;

[0014] The weight ratio of the active component A to the active component B is 9-20:1.

[0015] As a preferred technical solution of the present invention, the active component B is one or more of epoxiconazole and oxadiazole;

[0016] The weight ratio of the active component A to the active component B is 14.5-16.5:1.

[0017] As a preferred technical solution of the present invention, the active component B is hexaconazole;

[0018] The weight ratio of the active component A to the active component B is 18-20:1;

[0019] And / or, the active component B is prothioconazole;

[0020] The weight ratio of the active component A to the active component B is 15-20:1.

[0021] As a preferred technical solution of the present invention, the composition also contains an agriculturally acceptable carrier, which is selected from at least one of clay, silicates, silicon dioxide, resins, waxes, solid fertilizers, water, alcohols, ketones, petroleum fractions, aromatic hydrocarbons or wax hydrocarbons, chlorinated hydrocarbons, and liquefied gas.

[0022] The second aspect of the present invention provides a fungicide, which contains auxiliary materials and a fungicide-effective amount of active ingredients, and the active ingredient is the composition described in the first aspect.

[0023] The third aspect of the present invention provides an antifungal method, which comprises: applying the composition of the first aspect or the fungicide of the second aspect to crops.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] The pharmaceutical composition formed by compounding the active component A and the active component B in a specific content weight ratio (1.5-20:1) can effectively prevent and treat wheat stripe blight, and the effect is significantly better than that of a single agent, achieving a significant synergistic effect, thereby effectively expanding the scope of disease prevention and treatment, reducing the amount of medicine used, and delaying the development of disease resistance, which is of great significance to the sustainable development of agriculture.

[0026] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention, it can be implemented according to the contents of the specification. In order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are listed below. DETAILED DESCRIPTION

[0027] The first aspect of the present invention provides a pesticide composition for controlling wheat sheath blight, the composition comprising an active component A and an active component B; the active component A is a compound having a structure shown in formula (I),

[0028]

[0029] The active component B is selected from at least one of epoxiconazole, hexaconazole, metconazole and prothioconazole;

[0030] The weight ratio of the active component A to the active component B is 1.5-20:1.

[0031] Preferably, the active component B is one or more of epoxiconazole and metconazole;

[0032] The weight ratio of the active component A to the active component B is 9-20:1. Exemplarily, the weight ratio of the active component A to the active component B is 9:1, 10:1, 12:1, 15:1, 18:1, or 20:1.

[0033] Preferably, the active component B is one or more of epoxiconazole and metconazole;

[0034] The weight ratio of the active component A to the active component B is 14.5-16.5:1. The inventors found that under this preferred condition, the obtained pesticide composition has a better effect in preventing and controlling wheat sheath blight.

[0035] In order to obtain a pesticide composition with a better effect of preventing and controlling wheat sheath blight, preferably, the active component B is hexaconazole;

[0036] The weight ratio of the active component A to the active component B is 18-20:1.

[0037] Preferably, the active component B is prothioconazole;

[0038] The weight ratio of the active component A to the active component B is 15-20:1. The inventors found that under this preferred condition, the obtained pesticide composition has a better effect of preventing and controlling wheat sheath blight.

[0039] Preferably, the composition further comprises an agriculturally acceptable carrier.

[0040] The term "carrier" herein refers to an organic or inorganic, natural or synthetic substance. They facilitate the application of the active ingredient. The carrier is generally inert and must be agriculturally acceptable, especially to the treated plants. The carrier can be solid, such as clay, natural or synthetic silicates, silicon dioxide, resins, waxes, solid fertilizers, etc.; or liquid, such as water, alcohols, ketones, petroleum fractions, aromatic or waxy hydrocarbons, chlorinated hydrocarbons, liquefied gas, etc.

[0041] More preferably, the carrier is selected from at least one of clay, silicate, silicon dioxide, resin, wax, solid fertilizer, water, alcohols, ketones, petroleum fractions, aromatic hydrocarbons or wax hydrocarbons, chlorinated hydrocarbons, and liquefied gas.

[0042] As mentioned above, the second aspect of the present invention provides a fungicide, which contains auxiliary materials and a fungicide-effective amount of active ingredients, and the active ingredient is the composition described in the first aspect above.

[0043] Preferably, the auxiliary materials include but are not limited to: water, solvents, fillers, various surfactants (emulsifiers, dispersants, wetting agents, etc.), binders, film formers, colorants, dyes, antifreeze agents, thickeners, suspending agents, disintegrants, defoaming agents, permeants, synergists, stabilizers, wall capsule materials, pH adjusters, preservatives, etc.

[0044] Preferably, the content weight ratio of the active ingredient is 1-90wt%. Exemplarily, the content weight ratio of the active ingredient is 1wt%, 2wt%, 5wt%, 10wt%, 15wt%, 18wt%, 20wt%, 25wt%, 30wt%, 32wt%, 35wt%, 40wt%, 43wt%, 45wt%, 48wt%, 50wt%, 55wt%, 60wt%, 65wt%, 68wt%, 70wt%, 75wt%, 80wt%, 82wt%, 85wt%, 90wt%.

[0045] Preferably, the fungicide is in the form of at least one selected from wettable powder, suspension, water dispersible granules and aqueous emulsion.

[0046] The present invention has no particular limitation on the method for preparing the fungicide, and those skilled in the art can prepare the fungicide by conventional preparation methods in the field of pesticides to form the corresponding reagent.

[0047] As mentioned above, the third aspect of the present invention provides an antifungal method, which comprises: applying the composition described in the first aspect or the fungicide described in the second aspect to crops.

[0048] Preferably, the crops are selected from at least one of melon crops, solanaceous crops, bean crops, cereal crops, and vegetable crops.

[0049] More preferably, the crop is selected from at least one of wheat, cucumber, rapeseed, peanut, rice, potato, radish, carrot, lettuce, cabbage, grape, citrus, sugarcane, banana, pitaya, pepper, celery, citrus, watermelon and tomato.

[0050] The present invention will be described in detail below by way of examples. In the following examples, unless otherwise specified, the raw materials used are all common commercially available products.

[0051] In the following examples, unless otherwise specified, the percentages in the following formulas all represent percentages by weight.

[0052] The present invention has no particular limitation on the method for preparing the compound of the structure represented by formula (I). Those skilled in the art can obtain the compound of the structure represented by formula (I) according to the specific structural formula provided by other inventions in combination with the known process routes in the field of organic synthesis.

[0053] Test object: wheat sheath blight pathogen;

[0054] Fluoxepinazole, metconazole, hexaconazole and prothioconazole were all of analytical grade and purchased from Hubei Supu Chemical Co., Ltd.

[0055] Preparation Example

[0056] Active component A, epoxiconazole, hexaconazole, metconazole and prothioconazole were respectively prepared into solutions with a concentration of 10 mg / ml using dimethyl sulfoxide (DMSO), and the active component A solution (10 mg / ml) was mixed with epoxiconazole solution (10 mg / ml), hexaconazole solution (10 mg / ml), metconazole solution (10 mg / ml) and prothioconazole solution (10 mg / ml) according to the content weight ratio shown in Table 1.

[0057] Table 1: Preparation ratio of active component A

[0058]

[0059]

[0060] Test Case

[0061] Test for inhibiting the growth of pathogenic fungi hyphae - plate method: refer to "Agricultural Industry Standard of the People's Republic of China NY / T1156.2-2006"; first, set 6 different concentration gradients of the single agent and each fungicide to be mixed in a geometric progression with an inhibition rate within the range of 5%-90% (for specific experimental methods, refer to the above-mentioned "Agricultural Industry Standard of the People's Republic of China NY / T1156.2-2006"), and set up a water control. Refer to the "Pesticide Indoor Bioassay Test Guidelines for Fungicides" to determine the toxicity of the agent to crop pathogens. After 72 hours, the colony diameter was measured by the cross method, and the net growth, hyphae growth inhibition rate, EC50 value and co-toxicity coefficient (CTC) of each group were calculated. The calculation formula is as follows:

[0062] Net growth (mm) = measured colony diameter (mm) - bacterial cake diameter 5 mm; (the bacterial cake diameter refers to the diameter of the bacterial cake inoculated on the culture medium. In the present invention, the bacterial cake diameter is 5 mm);

[0063] Mycelial growth inhibition rate (%) = (net growth of control colonies - net growth of drug-treated colonies) / (net growth of control colonies) * 100;

[0064] EC50 value: through the biostatistical probability value conversion table, the probability value corresponding to the mycelium growth inhibition rate is assumed to be y, the drug concentration (μg / mL) is converted into a logarithmic value x, and the toxicity regression equation y=a+bx is obtained by the least squares method, and the EC50 value of each drug is calculated accordingly;

[0065] The results were determined according to Sun Yunpei's co-toxicity coefficient (CTC) method to determine the degree of synergy, i.e., CTC≤80 was antagonistic, 80<CTC<120 was additive, 120≤CTC<200 was synergistic, and CTC≥200 was significant synergistic. The highest co-toxicity coefficient had the strongest synergistic effect.

[0066] The co-toxicity coefficient is calculated according to the following formula:

[0067] Single-dose toxicity index (TI) = (standard fungicide EC50 / single-dose EC50) × 100;

[0068] The measured toxicity index (ATI) of the mixture = (EC50 of the standard fungicide / EC50 of the mixture) × 100;

[0069] Theoretical toxicity index (TTI) of mixture = toxicity index of agent A × content of agent A in the mixture (%) + toxicity index of agent B × content of agent B in the mixture (%);

[0070] The co-toxicity coefficient (CTC) of the mixture = (measured toxicity index of the mixture / theoretical toxicity index of the mixture) × 100;

[0071] The standard fungicide in the formula refers to the active component A;

[0072] The experimental results are shown in Table 2.

[0073] Table 2: Activity test results of active component A and active component B against wheat sheath blight.

[0074]

[0075]

[0076]

[0077] It can be seen from the results in Table 1 that the pharmaceutical composition obtained by using the active component A and the active component B in the present invention at a specific compounding ratio has a significant synergistic effect and can effectively prevent wheat sheath blight; among them, the synergistic effect of active component A and prothioconazole is the most obvious, and the antibacterial activity is the best.

[0078] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0079] It should be understood that the specific order or hierarchy of steps in the disclosed process is an example of an exemplary method. Based on design preferences, it should be understood that the specific order or hierarchy of steps in the process can be rearranged without departing from the scope of protection of the present disclosure. The attached method claims present the elements of the various steps in an exemplary order and are not intended to be limited to the specific order or hierarchy described.

[0080] In the above detailed description, various features are grouped together in a single embodiment to simplify the disclosure. This method of disclosure should not be interpreted as reflecting an intention that the embodiments of the claimed subject matter require more features than are clearly stated in each claim. On the contrary, as reflected in the appended claims, the invention is in a state of having less than all the features of the disclosed individual embodiments. Therefore, the appended claims are hereby expressly incorporated into the detailed description, with each claim standing on its own as a separate preferred embodiment of the invention.

Claims

1. A pesticide composition for preventing and treating wheat sheath blight, characterized in that: The composition comprises an active component A and an active component B; the active component A is a compound having a structure shown in formula (I), The active component B is selected from at least one of epoxiconazole, hexaconazole, metconazole and prothioconazole; The weight ratio of the active component A to the active component B is 1.5-20:

1.

2. A pesticide composition for preventing and controlling wheat sheath blight according to claim 1, characterized in that: The active component B is one or more of epoxiconazole and metconazole; The weight ratio of the active component A to the active component B is 9-20:

1.

3. A pesticide composition for controlling wheat sheath blight according to claim 1 or 2, characterized in that: The active component B is one or more of epoxiconazole and metconazole; The weight ratio of the active component A to the active component B is 14.5-16.5:

1.

4. A pesticide composition for controlling wheat sheath blight according to claim 1 or 2, characterized in that: The active component B is hexaconazole; The weight ratio of the active component A to the active component B is 18-20:1; And / or, the active component B is prothioconazole; the weight ratio of the active component A to the active component B is 15-20:

1.

5. The pesticide composition for preventing and controlling wheat sheath blight according to claim 4, characterized in that: The composition also contains an agriculturally acceptable carrier, which is selected from at least one of clay, silicate, silicon dioxide, resin, wax, solid fertilizer, water, alcohols, ketones, petroleum fractions, aromatic hydrocarbons or wax hydrocarbons, chlorinated hydrocarbons, and liquefied gas.

6. A bactericide, characterized in that: The bactericide contains auxiliary materials and a bactericidal effective amount of active ingredients, and the active ingredient is the composition described in claim 5.

7. A fungicide according to claim 6, characterized in that: The active ingredient has a content weight ratio of 1-90 wt %.

8. A fungicide according to claim 6 or 7, characterized in that: The dosage form of the fungicide is selected from at least one of wettable powder, suspension, water dispersible granules and aqueous emulsion.

9. An antifungal method, characterized in that: The method comprises: applying the composition according to any one of claims 1, 2 or 5 or the fungicide according to any one of claims 6 or 7 to crops.

10. An antifungal method according to claim 9, characterized in that: The crops are selected from at least one of melon crops, solanaceous crops, bean crops, cereal crops, and vegetable crops, and the crops are selected from at least one of wheat, cucumber, rapeseed, peanuts, rice, potatoes, radishes, carrots, lettuce, cabbage, grapes, citrus, sugarcane, bananas, pitaya, peppers, celery, citrus, watermelon, and tomatoes.

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