Natural plant anti-dandruff composition as well as preparation method and application thereof

Abstract

The invention discloses a natural plant anti-dandruff composition as well as a preparation method and application thereof, and relates to the technical field of daily necessities. The natural plant anti-dandruff composition comprises the following components: a chamaecyparis obtusa extract and a camellia seed cake extract, wherein the total mass of the natural plant anti-dandruff composition is 100%, the content of the chamaecyparis obtusa extract is 10-90%, and the content of the camellia seed cake extract is 10-90%. The raw materials of the natural plant anti-dandruff composition are natural and easy to obtain, the preparation method is green and safe, and the prepared composition has multiple effects of removing dandruff, controlling oil, relieving itching and the like, can effectively control dandruff, is friendly to scalp, does not irritate the scalp, is not prone to generating drug resistance, and has a good application prospect. The method has good efficacy, safety and practical feasibility.

Description

Technical Field

[0001] The invention relates to the technical field of daily necessities, and in particular to a natural plant anti-dandruff composition and a preparation method and application thereof. Background Art

[0002] Dandruff is a problem that bothers many people, and often when we find ourselves troubled by dandruff and take measures to prevent it, we often find that the dandruff comes back again and again and is difficult to completely remove. The reason for this is that, on the one hand, the dandruff problem is actually a complex and comprehensive problem: the factors that cause dandruff mainly include vigorous oil secretion, excessive reproduction of Malassezia, and individual susceptibility (fragile skin barrier). In addition, various internal and external factors such as chemical factors, physical factors, microorganisms and irritants will further aggravate the production of dandruff. Therefore, if we suffer from various scalp irritations while undergoing dandruff treatment, our dandruff problem will naturally be difficult to eradicate; on the other hand, the periodicity, persistence and recurrence of dandruff are also related to the current Most of the anti-dandruff products on the market use anti-dandruff agents: the mainstream anti-dandruff agents currently used on the market are still based on chemical ingredients such as selenium disulfide, salicylic acid, pyrithione zinc, and piroctone ethanolamine salt. Long-term use of chemical anti-dandruff agents will cause scalp irritation due to the anti-dandruff agents themselves or the chemical impurities remaining in the anti-dandruff agents. On the other hand, it may also produce a certain drug resistance, thereby weakening its anti-dandruff effect. In addition, some anti-dandruff shampoo and conditioner products only achieve anti-dandruff effects by inhibiting the reproduction of Malassezia, while ignoring the health of the scalp itself, which will also lead to poor or repeated anti-dandruff effects. Therefore, it is very necessary to develop a plant anti-dandruff raw material that is mild and non-irritating to the scalp.

[0003] Although natural plant antidandruff agents have received a lot of attention at present, many of them still have problems such as low content of active ingredients, unclear antidandruff mechanism, and poor antidandruff effect.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a natural plant anti-dandruff composition and a preparation method and application thereof.

[0006] The present invention is achieved in that:

[0007] In the first aspect, the present invention provides a natural plant anti-dandruff composition, whose components include cypress extract and camellia seed cake extract; wherein, based on the total mass of the natural plant anti-dandruff composition as 100%, the content of the cypress extract is 10-90%, and the content of the camellia seed cake extract is 10-90%.

[0008] In an optional embodiment, the preparation method of the camellia seed cake extract comprises the following steps:

[0009] S1: drying the tea seed meal left after oil extraction, and then crushing and sieving;

[0010] S2: Take 100g of tea seed meal crushed sample, add ethanol solution, soak and extract for 20-40min, then perform ultrasonic assisted extraction for 30-60min, filter, extract 2-4 times, combine the filtrate, and concentrate under reduced pressure;

[0011] S3: Freeze the concentrated sample, separate the emulsion layer, perform HP-20 macroporous resin column chromatography on the supernatant, fully elute with water, then elute with ethanol aqueous solution, and finally freeze-dry the eluate to obtain the camellia seed cake extract.

[0012] In an optional embodiment, the solid-liquid ratio of the crushed tea seed meal sample to the ethanol solution is 1g:5-25ml, the concentration of the ethanol solution is 30%-80%, and the temperature of the soaking extraction and the ultrasound-assisted extraction is 40-70°C;

[0013] And / or, the particle size of the crushed tea seed meal sample is 100-200 mesh.

[0014] In an optional embodiment, the method for preparing the Chamaecyparis obtusifolia extract comprises the following steps:

[0015] S1: Dry the discarded cypress wood and sawdust after wood processing, crush and sieve;

[0016] S2: Take 100g of crushed sample of Chamaecyparis obtusifolia, add anhydrous ethanol, seal and soak for 10-15h, ultrasonically treat for 40-50min, reflux extraction 2-4 times, and concentrate under reduced pressure to recover ethanol to obtain Chamaecyparis obtusifolia extract.

[0017] In an optional embodiment, the solid-liquid ratio of the crushed cypress sample and the anhydrous ethanol is 1g:5-25ml, the temperature of the reflux extraction is 40-70°C, and the extraction time is 10-60min;

[0018] And / or, the particle size of the crushed cypress sample is 100-200 mesh;

[0019] In a second aspect, the present invention provides a method for preparing a natural plant anti-dandruff composition, which comprises mixing the components of the natural plant anti-dandruff composition according to any one of the aforementioned embodiments.

[0020] In a third aspect, the present invention provides a use of the natural plant anti-dandruff composition according to any one of the aforementioned embodiments in the preparation of a hair care product;

[0021] Preferably, the hair care product comprises one or more of shampoo, conditioner, hair mask and scalp essence.

[0022] In a fourth aspect, the present invention provides an anti-dandruff shampoo, comprising the natural plant anti-dandruff composition according to any one of the aforementioned embodiments; preferably, based on the total mass of the anti-dandruff shampoo being 100%, the content of the natural plant anti-dandruff composition is 0.1-10%.

[0023] In an optional embodiment, the anti-dandruff shampoo further comprises one or a combination of two or more of a surfactant, a hair conditioner, a preservative, a pH adjuster, a fragrance, a thickener, and a solvent water;

[0024] Preferably, the surfactant is selected from sodium laureth sulfate and cocamidopropyl betaine;

[0025] Preferably, the hair conditioning agent is selected from guar hydroxypropyltrimonium chloride;

[0026] Preferably, the preservative is selected from phenoxyethanol and sodium benzoate;

[0027] Preferably, the pH adjuster is selected from citric acid;

[0028] Preferably, the thickener is selected from cocamide MEA and sodium chloride.

[0029] In an optional embodiment, the preparation method of the anti-dandruff shampoo comprises the following steps:

[0030] S1: Add water into an emulsifier and heat to 75-85°C, add sodium laureth sulfate and cocamidopropyl betaine, and stir until completely dissolved;

[0031] S2: Add guar hydroxypropyltrimethylammonium chloride and cocamide MEA pre-dispersed in cold water, and stir at the temperature for 20-40 minutes until the mixture is uniform;

[0032] S3: Cool down to 40-50℃, add sodium benzoate, phenoxyethanol, citric acid and essence, and stir evenly;

[0033] S4: adding the natural plant anti-dandruff composition and stirring evenly;

[0034] S5: Add sodium chloride to adjust the viscosity, stir until the material is uniform and then filter out the material.

[0035] The present invention has the following beneficial effects:

[0036] The natural plant anti-dandruff composition provided by the present invention is prepared by mixing Chamaecyparis obtusifolia extract and camellia seed cake extract in a certain proportion, wherein the component raw materials are respectively derived from the waste tea seed meal left after tea seed oil extraction and the waste wood and sawdust after Chamaecyparis obtusifolia wood processing, which can not only utilize waste and reduce the over-exploitation of natural resources, but also save costs and improve the practicality and economy of the plant anti-dandruff composition. The plant anti-dandruff composition prepared by the present application adds the steps of pretreatment such as crushing, sieving, extraction, and ultrasound, which is beneficial to increase the content of active ingredients in the extract, thereby increasing the efficacy of the composition. The present application optimizes the extraction conditions, extracts tea seed meal and Chamaecyparis obtusifolia separately, and adds the separation and purification operations of tea seed meal, which is beneficial to reduce the pigments, impurities and metal ions in the extract, thereby increasing the content and biological activity of active substances such as tea polyphenols and Chamaecyparis obtusifolia, such as reducing the influence of metal iron ions in the tea seed cake extract on Chamaecyparis obtusifolia in the Chamaecyparis obtusifolia extract. Furthermore, the plant anti-dandruff composition prepared in the present application has a green and environmentally friendly extraction method, does not introduce any toxic solvents or difficult-to-handle acid-base reagents, greatly improves the safety of the composition, and through the reasonable compounding of two plant extracts, can effectively control dandruff at a relatively small dosage, and is scalp-friendly, does not cause irritation to the scalp, and is not easy to produce drug resistance. It can be widely used in the preparation of hair care products. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0038] The invention provides a natural plant anti-dandruff composition, the components of which include cypress extract and camellia seed cake extract; wherein, based on the total mass of the natural plant anti-dandruff composition being 100%, the content of the cypress extract is 10-90%, and the content of the camellia seed cake extract is 10-90%.

[0039] In the present invention, the cypress extract and the camellia seed cake extract are compounded in a certain ratio, wherein the cypress extract contains active ingredients such as cypressol, has excellent antibacterial properties, can effectively inhibit the excessive reproduction of Malassezia, and has good safety; the tea saponins, tea polyphenols, proteins, polysaccharides and other ingredients in the camellia seed cake extract can play the role of antibacterial, anti-inflammatory, anti-oxidation, oil control and moisturizing. The two natural plant extracts can be reasonably compounded to effectively control dandruff, and are scalp-friendly, will not cause irritation to the scalp, and are not easy to develop drug resistance.

[0040] Preferably, based on 100% of the total mass of the natural plant anti-dandruff composition, the content of Chamaecyparis obtusifolia extract is 30-70%, and the content of Camellia japonica seed cake extract is 30-70%.

[0041] The preparation method of camellia seed cake extract comprises the following steps:

[0042] S1: The tea seed meal left after oil extraction is dried, crushed and sieved.

[0043] The drying temperature may be, for example, 70-90° C. After drying, the mixture is cooled to room temperature, and then crushed by a crusher and passed through a 100-200 mesh sieve for later use.

[0044] S2: Take 100g of crushed tea seed meal sample, add ethanol solution, soak and extract for 20-40min, then perform ultrasonic-assisted extraction for 30-60min, filter, extract 2-4 times, combine the filtrate, and concentrate under reduced pressure.

[0045] The solid-liquid ratio of the crushed tea seed meal sample and the ethanol solution is 1g:5-25ml, the concentration of the ethanol solution is 30%-80%, and the temperature of the soaking extraction and the ultrasonic-assisted extraction is 40-70°C; in the present invention, the crushed tea seed meal sample is soaked and extracted and ultrasonic-assisted extracted by the ethanol solution, which can extract the effective components in the crushed tea seed meal sample to the maximum extent.

[0046] S3: Freeze the concentrated sample, separate the emulsion layer, perform HP-20 macroporous resin column chromatography on the supernatant, fully elute with water, then elute with ethanol aqueous solution, and finally freeze-dry the eluate to obtain the camellia seed cake extract.

[0047] In the present invention, the concentrated sample is first frozen and the emulsion layer is separated, wherein the HP-20 macroporous resin column is used for column chromatography to fully separate and purify the effective ingredients in the supernatant, thereby improving the purity and active ingredient content of the camellia seed cake extract, reducing the impurities in the extract, and improving the anti-dandruff effect.

[0048] In the present invention, the camellia seed cake extract obtained by the preparation method of the camellia seed cake extract can increase the antioxidant, anti-inflammatory and oil control effects of the anti-dandruff composition without affecting the antibacterial effect of the cypress extract, thereby increasing the mildness of the composition and increasing the action pathway of the anti-dandruff mechanism. The extraction method of the present invention is to pulverize and then extract with alcohol and purify, which can improve the purity and active ingredient content of the camellia seed cake extract, reduce the impurities of the extract, and avoid the influence of metal ions on the antibacterial effect of the cypress extract.

[0049] In addition, the camellia seed cake extract in the present invention cannot be extracted by hot water decoction. According to the literature, in addition to the desired active ingredients such as tea saponins and tea polyphenols, hot water extraction will also produce a large amount of starch, protein, sugars and other ingredients that contribute relatively little to the anti-dandruff effect. Too many and too complex components are not conducive to improving the quality of the extract, and will have a certain impact on the antibacterial effect of the cypress extract.

[0050] The preparation method of Chamaecyparis obtusifolia extract comprises the following steps:

[0051] S1: The discarded cypress wood and sawdust after wood processing are dried, crushed and sieved.

[0052] When the discarded cypress wood is large in size, it can be crushed into small particles and then dried. The drying temperature can be, for example, 70-90°C. After drying, it is cooled to room temperature and then crushed with a grinder and sieved through a 100-200 mesh sieve for later use.

[0053] S2: Take 100g of crushed sample of Chamaecyparis obtusifolia, add anhydrous ethanol, seal and soak for 10-15h, ultrasonically treat for 40-50min, reflux extraction 2-4 times, and concentrate under reduced pressure to recover ethanol to obtain Chamaecyparis obtusifolia extract.

[0054] In the present invention, anhydrous ethanol is used to extract the cypress crushed sample, and during the reflux extraction process, the solvent volatilization loss can be avoided, and the extraction efficiency is high, and the effective components in the cypress crushed sample can be fully extracted. Specifically, the solid-liquid ratio of the cypress crushed sample and anhydrous ethanol is 1g:5-25ml, the reflux extraction temperature is 40-70℃, and the extraction time is 10-60min.

[0055] The natural plant anti-dandruff composition of the present invention is respectively derived from the waste tea seed meal left after tea seed oil extraction and the waste wood and sawdust after cypress wood processing, which can not only utilize waste and reduce the over-exploitation of natural resources, but also save costs and improve the practicality and economy of the plant anti-dandruff composition. Before the extraction of the cypress extract and the camellia seed cake extract of the present invention, the steps of pretreatment such as crushing, screening, leaching, and ultrasound are added, which is beneficial to increase the content of active ingredients in the extract, thereby increasing the efficacy of the composition. In addition, in the present invention, the tea seed meal and the cypress are extracted separately, and the separation and purification operation of the tea seed meal is added, which is beneficial to reduce the pigments, impurities and metal ions in the extract, thereby increasing the content and biological activity of active substances such as tea polyphenols and cypress phenol, such as reducing the influence of metal iron ions in the tea seed cake extract on cypress phenol in the cypress extract.

[0056] Furthermore, the present invention provides a method for preparing a natural plant anti-dandruff composition, which comprises mixing the components of the natural plant anti-dandruff composition.

[0057] The plant anti-dandruff composition prepared in the present application has a green and environmentally friendly extraction method, does not introduce any toxic solvents or difficult-to-handle acid-base reagents, greatly improves the safety of the composition, and through the reasonable compounding of two plant extracts, it can achieve effective control of dandruff at a relatively small dosage, and is scalp-friendly, will not cause irritation to the scalp, and is not easy to develop drug resistance.

[0058] In addition, the present invention also provides the use of the above-mentioned natural plant anti-dandruff composition in the preparation of hair care products; preferably, the hair care products include one or more of shampoo, conditioner, hair mask and scalp essence.

[0059] Specifically, the present invention provides a typical but non-limiting example: an anti-dandruff shampoo, comprising the above-mentioned natural plant anti-dandruff composition; preferably, based on the total mass of the anti-dandruff shampoo as 100%, the content of the natural plant anti-dandruff composition is 0.1-10%, and more preferably 0.5-5%.

[0060] The anti-dandruff shampoo also includes one or a combination of two or more of a surfactant, a hair conditioner, a preservative, a pH adjuster, a fragrance, a thickener and a solvent, water.

[0061] Specifically, the anti-dandruff shampoo contains the following substances in percentage by weight:

[0062]

[0063]

[0064] The surfactant is selected from: sodium lauryl sulfate, sodium laureth sulfate, ammonium lauryl sulfate, ammonium laureth sulfate, sodium laureth-6 carboxylate, disodium laureth sulfosuccinate, sodium C14-16 olefin sulfonate, sodium lauroyl glutamate, disodium lauroyl glutamate, TEA lauroyl glutamate, sodium cocoyl glutamate, disodium cocoyl glutamate, TEA cocoyl glutamate, sodium lauroyl sarcosinate, sodium cocoyl sarcosinate, myristoyl sarcosine The surfactant is selected from sodium methyl cocoyl taurate, sodium cocoyl methyl taurate, sodium cocoyl amino propionate, potassium cocoyl hydrolyzed oat protein, lauryl betaine, cocoyl betaine, lauryl hydroxysultaine, cocoyl hydroxysultaine, cocamidopropyl betaine, sodium lauroamphoacetate, sodium cocoamphoacetate, disodium lauroamphodiacetate, disodium cocoamphodiacetate, decyl glucoside, lauryl glucoside, and one or more of cocoyl glucoside. Preferably, the surfactant is selected from sodium laureth sulfate and cocamidopropyl betaine.

[0065] The hair conditioning agent is selected from: one or more of polyquaternium-7, polyquaternium-10, polyquaternium-11, polyquaternium-39, polyquaternium-47, polyquaternium-53, polyquaternium-67, polyquaternium-73, polyquaternium-74, guar hydroxypropyltrimonium chloride, hydroxypropyl guar hydroxypropyltrimonium chloride, stearamidopropyl dimethylamine, polydimethylsiloxane, polydimethylsiloxane alcohol, amino-terminated polydimethylsiloxane, PPG-3 caprylyl ether, C12-13 alcohol lactate, PEG-7 glyceryl cocoate, glyceryl laurate, PPG-14 butyl ether, di(PPG-2 myristeth-10) adipate, and caprylic / capric triglyceride; preferably, the hair conditioning agent is selected from guar hydroxypropyltrimonium chloride.

[0066] The preservative is selected from at least one of methylchloroisothiazolinone, methylisothiazolinone, DMDM ​​hydantoin, bis(hydroxymethyl)imidazolidinyl urea, dichlorobenzyl alcohol, potassium sorbate, sodium salicylate, chlorphenesin, phenoxyethanol, sodium benzoate, benzyl alcohol, ethylhexylglycerin, methylparaben, p-hydroxyacetophenone, and 1,2-hexanediol. Preferably, the preservative is selected from phenoxyethanol and sodium benzoate.

[0067] The pH adjuster is selected from: one or more of citric acid, sodium citrate, sodium hydroxide, potassium hydroxide, triethanolamine, aminomethyl propanol, and arginine; preferably, the pH adjuster is selected from citric acid.

[0068] The thickener is selected from: one or more of PEG-150 distearate, PEG-120 methyl glucose dioleate, hydroxypropyl methylcellulose, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, acrylic acid (esters) copolymer, carbomer, cocamide methyl MEA, cocamide MEA, cocamide DEA, and sodium chloride; preferably, the thickener is selected from cocamide MEA and sodium chloride.

[0069] The preparation method of the anti-dandruff shampoo comprises the following steps:

[0070] S1: Add water into an emulsifier and heat to 75-85°C, add sodium laureth sulfate and cocamidopropyl betaine, and stir until completely dissolved;

[0071] S2: Add guar hydroxypropyltrimethylammonium chloride and cocamide MEA pre-dispersed in cold water, and stir at the temperature for 20-40 minutes until the mixture is uniform;

[0072] S3: Cool down to 40-50℃, add sodium benzoate, phenoxyethanol, citric acid and essence, and stir evenly;

[0073] S4: adding the natural plant anti-dandruff composition and stirring evenly;

[0074] S5: Add sodium chloride to adjust the viscosity, stir until the material is uniform and then filter out the material.

[0075] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0076] Example 1

[0077] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0078] (1) Preparation of camellia seed cake extract.

[0079] The tea seed meal remaining after oil extraction is dried at a constant temperature of 80°C, crushed by a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 60% ethanol is added according to a solid-liquid ratio of 1:15, the sample is sealed, and the mixture is leached at 70°C for 30 minutes, and ultrasonic-assisted extraction is performed for 40 minutes, and the sample is filtered, and the filtrate is combined after extraction for 3 times, and the sample is concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, the ethanol / water solution is used for elution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0080] (2) Preparation of Chamaecyparis obtusifolia extract.

[0081] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:5, and the mixture was sealed and soaked for 12 hours, then ultrasonically treated for 30 minutes, refluxed and extracted at 40°C for 30 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0082] (3) Prepare a natural plant anti-dandruff composition.

[0083] The prepared Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract were mixed evenly in mass percentages of 90% and 10% to obtain a natural plant anti-dandruff composition, which was then put into a brown bottle and stored in a cool and dry place for later use.

[0084] Example 2

[0085] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0086] (1) Preparation of camellia seed cake extract.

[0087] The tea seed meal remaining after oil extraction is dried at a constant temperature of 80°C, crushed by a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 60% ethanol is added according to a solid-liquid ratio of 1:15, the sample is sealed, and the mixture is leached at 50°C for 30 minutes, and ultrasonic-assisted extraction is performed for 40 minutes, and the sample is filtered, and the filtrate is combined after extraction for 3 times, and the filtrate is concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, it is eluted with ethanol / water solution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0088] (2) Preparation of Chamaecyparis obtusifolia extract.

[0089] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:20, and the mixture was sealed and soaked for 12 hours, then ultrasonically treated for 30 minutes, refluxed and extracted at 70°C for 30 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0090] (3) Prepare a natural plant anti-dandruff composition.

[0091] The prepared Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract were mixed evenly in a mass percentage of 70% and 30% to obtain a natural plant anti-dandruff composition, which was put into a brown bottle and stored in a cool and dry place for later use.

[0092] Example 3

[0093] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0094] (1) Preparation of camellia seed cake extract.

[0095] The tea seed meal remaining after oil extraction is dried at a constant temperature of 80°C, crushed by a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 60% ethanol is added according to a solid-liquid ratio of 1:20, the sample is sealed, and the mixture is extracted at 50°C for 30 minutes, and ultrasonic-assisted extraction is performed for 40 minutes, and the sample is filtered, and the filtrate is combined after extraction for 3 times, and the mixture is concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, the ethanol / water solution is used for elution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0096] (2) Preparation of Chamaecyparis obtusifolia extract.

[0097] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:20, sealed and soaked for 12 hours, ultrasonically treated for 30 minutes, refluxed at 60°C for 30 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0098] (3) Prepare a natural plant anti-dandruff composition.

[0099] The prepared Chamaecyparis obtusifolia extract and Camellia seed cake extract were mixed evenly in a mass percentage of 50% and 50% to obtain a natural plant anti-dandruff composition, which was put into a brown bottle and stored in a cool and dry place for later use.

[0100] Example 4

[0101] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0102] (1) Preparation of camellia seed cake extract.

[0103] The tea seed meal left after oil extraction is dried at a constant temperature of 80°C, crushed by a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 30% ethanol is added according to a solid-liquid ratio of 1:10, the sample is sealed, and the mixture is leached at 50°C for 30 minutes, and ultrasonic-assisted extraction is performed for 40 minutes, and the sample is filtered, and the filtrate is combined after extraction for 3 times, and the sample is concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, the ethanol / water solution is used for elution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0104] (2) Preparation of Chamaecyparis obtusifolia extract.

[0105] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:10, sealed and soaked for 12 hours, ultrasonically treated for 30 minutes, refluxed at 70°C for 30 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0106] (3) Prepare a natural plant anti-dandruff composition.

[0107] The prepared Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract were uniformly mixed in a mass percentage of 30% and 70% to obtain a natural plant anti-dandruff composition, which was then put into a brown bottle and stored in a cool and dry place for later use.

[0108] Example 5

[0109] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0110] (1) Preparation of camellia seed cake extract.

[0111] The tea seed meal remaining after oil extraction is dried at a constant temperature of 80°C, crushed by a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 60% ethanol is added according to a solid-liquid ratio of 1:25, the sample is sealed, and the mixture is leached at 40°C for 30 minutes, and then ultrasonic-assisted extraction is performed for 40 minutes, and the sample is filtered, and the filtrate is combined after extraction for 3 times, and the sample is concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, the ethanol / water solution is used for elution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0112] (2) Preparation of Chamaecyparis obtusifolia extract.

[0113] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:15, and the mixture was sealed and soaked for 12 hours, then ultrasonically treated for 30 minutes, refluxed and extracted at 70°C for 60 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0114] (3) Prepare a natural plant anti-dandruff composition.

[0115] The prepared Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract were mixed evenly in a mass percentage of 70% and 30% to obtain a natural plant anti-dandruff composition, which was put into a brown bottle and stored in a cool and dry place for later use.

[0116] Example 6

[0117] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0118] (1) Preparation of camellia seed cake extract.

[0119] The tea seed meal remaining after oil extraction is dried at a constant temperature of 80°C, crushed with a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 60% ethanol is added according to a solid-liquid ratio of 1:5, the sample is sealed, and the mixture is leached at 50°C for 30 minutes, followed by ultrasonic-assisted extraction for 40 minutes, and filtered, and the filtrate is combined after extraction for 3 times, and concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, it is eluted with ethanol / water solution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0120] (2) Preparation of Chamaecyparis obtusifolia extract.

[0121] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:20, sealed and soaked for 12 hours, ultrasonically treated for 30 minutes, refluxed at 50°C for 10 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0122] (3) Prepare a natural plant anti-dandruff composition.

[0123] The prepared Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract were mixed evenly in mass percentages of 10% and 90% to obtain a natural plant anti-dandruff composition, which was then put into a brown bottle and stored in a cool and dry place for later use.

[0124] Example 7

[0125] This embodiment provides a natural plant anti-dandruff composition, and the preparation method thereof comprises:

[0126] (1) Preparation of camellia seed cake extract.

[0127] The tea seed meal remaining after oil extraction is dried at a constant temperature of 80°C, crushed by a pulverizer after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample is taken, 80% ethanol is added according to a solid-liquid ratio of 1:15, the sample is sealed, and the mixture is leached at 60°C for 30 minutes, and ultrasonic-assisted extraction is performed for 40 minutes, and the sample is filtered, and the filtrate is combined after extraction for 3 times, and the filtrate is concentrated under reduced pressure; the concentrated sample is frozen in a refrigerator, and the emulsion layer is separated, and the obtained supernatant is subjected to HP-20 macroporous resin column chromatography, and after sufficient water elution, it is eluted with ethanol / water solution, and finally the eluate is freeze-dried to obtain a camellia seed cake extract.

[0128] (2) Preparation of Chamaecyparis obtusifolia extract.

[0129] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:25, and the mixture was sealed and soaked for 12 hours, then ultrasonically treated for 30 minutes, refluxed and extracted at 60°C for 30 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0130] (3) Prepare a natural plant anti-dandruff composition.

[0131] The prepared Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract were mixed evenly in a mass percentage of 70% and 30% to obtain a natural plant anti-dandruff composition, which was put into a brown bottle and stored in a cool and dry place for later use.

[0132] Comparative Example 1

[0133] Camellia seed cake extract is used as an anti-dandruff agent. The preparation method of camellia seed cake extract is the same as step (1) in Example 2.

[0134] Comparative Example 2

[0135] The Chamaecyparis obtusifolia extract is used as an anti-dandruff agent. The preparation method of the Chamaecyparis obtusifolia extract is the same as step (2) in Example 2.

[0136] Comparative Example 3

[0137] The cypress-tea seed bran extract was prepared as an anti-dandruff agent with reference to the preparation method of the cypress-tea seed bran extract disclosed in patent CN 105362108 A. The specific preparation method is as follows:

[0138] The Chamaecyparis obtusifolia-tea seed bran extract was extracted with ethanol as a solvent at a mass ratio of 8:1 and refluxed at 70°C for 30 minutes at a solid-liquid ratio of 17 g / L to obtain the Chamaecyparis obtusifolia-tea seed bran extract.

[0139] Comparative Example 4

[0140] This comparative example is basically the same as Example 2, except that in this comparative example, the product of reduced pressure concentration is directly used as a component in step (1). The preparation method comprises:

[0141] (1) Preparation of camellia seed cake crude extract.

[0142] The tea seed cake left after oil extraction was dried at a constant temperature of 80°C, crushed with a grinder after cooling, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was taken, 60% ethanol was added according to a solid-liquid ratio of 1:15, the sample was sealed, and the mixture was soaked at 50°C for 30 minutes, and then ultrasonic-assisted extraction was performed for 40 minutes, and the mixture was filtered, and the filtrate was combined after extraction for 3 times, and concentrated under reduced pressure to obtain a crude extract of camellia seed cake.

[0143] (2) Preparation of Chamaecyparis obtusifolia extract.

[0144] The discarded cypress wood and sawdust after wood processing were crushed by a crusher, dried at a constant temperature of 80°C, and passed through a 100-mesh sieve for later use; 100 g of the crushed sample was weighed, anhydrous ethanol was added according to a solid-liquid ratio of 1:20, and the mixture was sealed and soaked for 12 hours, then ultrasonically treated for 30 minutes, refluxed and extracted at 70°C for 30 minutes, extracted 3 times, and concentrated under reduced pressure to recover ethanol to obtain the cypress extract.

[0145] (3) Prepare a natural plant anti-dandruff composition.

[0146] The prepared cypress extract and camellia seed cake crude extract were evenly mixed in a mass percentage of 70% and 30% to obtain a natural plant anti-dandruff composition, which was put into a brown bottle and stored in a cool and dry place for later use.

[0147] Application Examples 1-7 and Application Comparative Examples 1-4

[0148] The natural plant anti-dandruff compositions prepared in Examples 1-7 and the products prepared in Comparative Examples 1-4 were added into the shampoo formula in Table 1 at a mass percentage of 5%. The anti-dandruff shampoo was prepared according to the following method.

[0149] The specific preparation method is: prepare each application example sample according to the formula ratio of Table 1, and prepare each application comparison example sample according to the formula of Table 2. The preparation method is:

[0150] S1: Add water into an emulsifier and heat to 75-85°C, add sodium laureth sulfate and cocamidopropyl betaine, and stir until completely dissolved;

[0151] S2: Add guar gum hydroxypropyltrimethylammonium chloride and cocamide MEA pre-dispersed in cold water, and stir for about 30 minutes to mix evenly;

[0152] S3: Cool down to 45°C, add sodium benzoate, phenoxyethanol, citric acid and essence, and stir evenly;

[0153] S4: adding the natural plant anti-dandruff composition and stirring evenly;

[0154] S5: Add sodium chloride to adjust the viscosity, stir until the material is uniform and then filter out the material.

[0155] Table 1. Shampoo formulas of application examples 1-7

[0156]

[0157] Table 2. Shampoo formulas for comparative examples 1-4

[0158] formula Application Comparative Example 1 Application Comparative Example 2 Application Comparative Example 3 Application Comparative Example 4 water To 100 To 100 To 100 To 100 Sodium Laureth Sulfate 13 13 13 13 Cocamidopropyl Betaine 5 5 5 5 Guar Hydroxypropyltrimonium Chloride 0.3 0.3 0.3 0.3 Cocamide MEA 1 1 1 1 Sodium Benzoate 0.3 0.3 0.3 0.3 Phenoxyethanol 0.3 0.3 0.3 0.3 Citric acid 0.1 0.1 0.1 0.1 essence 0.5 0.5 0.5 0.5 Camellia Seed Cake Extract 5 / / / Camellia seed cake crude extract / / / 1.5 Chamaecyparis Obtusa Extract / 5 / 3.5 Chamaecyparis obtusa-tea seed bran extract / / 5 / Sodium chloride 1 1 1 1

[0159] Control group

[0160] The control group was a commercially available anti-dandruff shampoo (Head & Shoulders Anti-Dandruff Shampoo).

[0161] Performance Testing

[0162] 1. In vitro antibacterial test

[0163] Test samples: Platycladus orientalis leaf extract (Beijing Oriental Miaosen Biotechnology Co., Ltd.), Comparative Examples 1-4 and Example 2.

[0164] Experimental content:

[0165] The antibacterial effect was detected by the inhibition ring method: the culture medium used was agar culture medium, and the test strain was the standard strain of Malassezia furfur.

[0166] Prepare a bacterial suspension of about 105 cfu of Malassezia furfur, evenly spread it on a Leeming & Notman medium plate, and then place it on a sterile operating table to blow dry the bacterial suspension on the plate.

[0167] The samples were quantitatively added to the above-mentioned plates, cultured in a constant temperature incubator at 30°C for 5-7 days, and the results were observed. The inhibition effect was evaluated by the diameter d (mm) of the inhibition zone according to the standard of the National Committee for Clinical Trials Standards (NCCLS) of the United States: d>19 was highly sensitive, recorded as ++++; 13≤d<19 was moderately sensitive, recorded as +++; 8≤d<13 was lowly sensitive, recorded as ++; 0<d<8 was insensitive, recorded as +; no inhibition was recorded as -.

[0168] Table 3. Inhibitory effects of different test samples on Malassezia

[0169] Serial number Test samples Inhibitory effect on Malassezia 1 5% Oriental Platycladus orientalis leaf extract -- 2 5% Comparative Example 1 (Camellia Seed Cake Extract) + 3 5% Comparative Example 2 (Chamaecyparis obtusifolia extract) ++++ 4 5% Comparative Example 3 (Chamaecyparis obtusifolia-tea seed bran extract) +++ 5 5% Comparative Example 4 (Chamaecyparis extract + camellia seed cake crude extract) +++ 6 5% Example 2 (Chamaecyparis obtusifolia extract + Camellia seed cake extract) ++++

[0170] It can be found from Table 3 that Platycladus orientalis leaf extract has no antibacterial effect on Malassezia at the test concentration, while the antibacterial effect of Chamaecyparis obtusifolia extract is very strong, which fully proves that the active ingredients of Platycladus orientalis leaf extract and Chamaecyparis obtusifolia extract are different. As can be seen from Comparative Example 1, a single camellia seed cake extract has a certain inhibitory effect on Malassezia, but the effect is very weak. As can be seen from Comparative Examples 3-4 and Example 2, it is not that Chamaecyparis obtusifolia and camellia seed compound can achieve a good antibacterial effect. The antibacterial effect of camellia seed cake extract and Chamaecyparis obtusifolia extract when acting together has a great relationship with the extraction process of camellia seed cake extract. The camellia seed cake extract obtained by extracting according to the method of Example 2 of the present invention can effectively reduce the negative impact of impurities such as metal ions in the tea seed cake extract on the antibacterial effect of active ingredients such as cypressol in Chamaecyparis obtusifolia extract, thereby maintaining the strong inhibitory effect of the composition on Malassezia.

[0171] 2. Comprehensive evaluation of anti-dandruff effect

[0172] Test samples: Application Examples 1-7, Application Comparative Examples 1-4 and a commercially available anti-dandruff shampoo.

[0173] Test method: 120 subjects aged between 18 and 50 with obvious dandruff and no head diseases such as psoriasis were selected. After receiving the samples, the subjects used the test samples once every 2 days. After using them for 2 consecutive weeks, the subjects evaluated according to their own experience. The evaluation levels were effective, general, and ineffective (for example, for the improvement of dandruff, effective means no dandruff or very little dandruff, general means a little reduction in dandruff, and ineffective means no reduction in dandruff).

[0174] Test results: There were 10 subjects in each group, and the number of effective, average and ineffective subjects was statistically evaluated, as shown in Table 4.

[0175] Table 4. Comprehensive evaluation of anti-dandruff effect

[0176]

[0177] As can be seen from each application example of Table 4, the natural plant anti-dandruff composition prepared by the present application not only has a good anti-dandruff effect, but also can effectively improve the conditions such as greasy scalp and itching. This is because the anti-dandruff composition of the present application not only has the effect of inhibiting the excessive reproduction of Malassezia, but also has the effects of oil control and moisturizing, anti-oxidation and anti-inflammatory, and multiple action mechanisms act on each period of dandruff production, which can not only effectively improve dandruff, but also can alleviate the scalp inflammation problem caused by greasy scalp and dandruff production. It can also be seen from Table 4 Application Example 2 and Application Comparative Examples 1-2 that to achieve such an effect, it is necessary for Chamaecyparis obtusifolia extract and Camellia seed cake extract to play a role together, and a single extract cannot achieve the anti-dandruff, oil control and antipruritic effects of the application example. And the results of the comparison of Application Example 2 and Application Comparative Example 4 can be seen that the Camellia seed cake extract is directly matched with Chamaecyparis obtusifolia extract in the form of crude extract without purification, and the anti-dandruff, oil control and soothing effects of its composition are far less than the effect of the purified Camellia seed cake extract and Chamaecyparis obtusifolia extract combination, indicating that the extraction process of Camellia seed cake extract has a great influence on the anti-dandruff, oil control and soothing effects of the composition.

[0178] It can be seen from the application comparison example 3 in Table 4 that the anti-dandruff effect of the cypress-tea seed bran extract prepared by the one-step method is far inferior to the natural plant anti-dandruff composition prepared in the present application at the same formula addition amount, which further illustrates that the preparation method of the composition of the present application is more scientific and effective, and the prepared composition has better anti-dandruff effect. The reason for this phenomenon may be that the one-step mixed extraction of the cypress-tea seed bran extract cannot better screen the optimal extraction conditions of the two compositions, resulting in a relatively low content of active substances, and a larger formula addition amount is required to produce a better anti-dandruff effect; on the other hand, the tea seed bran is not separated and purified during extraction, so it is impossible to obtain a better content of active ingredients, and it is impossible to effectively remove impurities such as metal ions in the extract. The presence of these metal ions, especially iron ions, will affect the activity of antibacterial components such as cypress phenol in the cypress extract, thereby reducing the anti-dandruff effect of the entire composition.

[0179] From the application examples 2-5 and the control group in Table 4, it can be seen that although the samples in the control group have good anti-dandruff and oil control effects, their antipruritic effect is slightly poor, which may be caused by the irritation of the samples in the control group to the scalp. By adjusting the preparation process of the anti-dandruff composition and the ratio of the Chamaecyparis obtusifolia extract and the tea seed cake extract, several application example samples can achieve multiple effective controls of the composition on dandruff, oily scalp and itchy scalp, indicating that the composition prepared in this application has a good application prospect.

[0180] 3. Human skin patch test

[0181] The shampoos prepared in Application Examples 1-7 and the shampoo base prepared in Application Examples without adding an anti-dandruff agent were subjected to a patch test to verify the irritation of the composition of the present application.

[0182] Experimental method: human skin patch test, the test method is based on: "Technical Specifications for Safety of Cosmetics" (2015 edition). This product is a shampoo, and hair cleansers are diluted into a 1% aqueous solution as the test substance. Select appropriate patch test equipment, use the closed patch test method, select at least 30 qualified volunteers to participate in the test, and add the test substance and deionized water control into the square chamber of the patch tester according to the random distribution of samples. Apply the patch test equipment with the test substance and the control sample to the inner side of the subject's forearm, and press it lightly with the palm of your hand to evenly apply it to the skin for 24 hours. Observe the skin reaction according to the standards in Table 5 30 minutes (after the indentation disappears), 24 hours and 48 hours after removing the test substance patch tester, and record the observation results. Please refer to Table 6 for the test results.

[0183] Table 5. Skin occlusion patch test skin reaction grading standard

[0184]

[0185] Table 6 Statistical table of human patch test results

[0186]

[0187]

[0188] It can be seen from Table 6 that when the shampoo base was subjected to the patch test, there were two cases of grade 1 reactions in 0.5 h, indicating that the shampoo base is irritating to human skin to a certain extent. Combining the patch test results of various application examples, the anti-dandruff composition prepared in the present application not only does not increase the irritation of shampoo to the scalp, but also can improve the irritation of the surfactant system to the scalp to a certain extent, indicating that the composition prepared in the present application is relatively mild and less irritating.

[0189] In summary, the natural plant anti-dandruff composition provided by the present invention is prepared by combining Chamaecyparis obtusifolia extract and Camellia japonica seed cake extract in a certain proportion, wherein the component raw materials are respectively derived from the waste tea seed meal left after tea seed oil extraction and the waste wood and sawdust after Chamaecyparis obtusifolia wood processing, which can not only utilize waste and reduce the over-exploitation of natural resources, but also save costs and improve the practicality and economy of the plant anti-dandruff composition. The plant anti-dandruff composition prepared by the present application adds the steps of pretreatment such as crushing, sieving, extraction, and ultrasound, which is beneficial to increase the content of active ingredients in the extract, thereby increasing the efficacy of the composition. The present application optimizes the extraction conditions, extracts tea seed meal and Chamaecyparis obtusifolia separately, and increases the separation and purification operations of tea seed meal, which is beneficial to reduce the pigments, impurities and metal ions in the extract, thereby increasing the content and biological activity of active substances such as tea polyphenols and Chamaecyparis obtusifolia, such as reducing the influence of metal iron ions in tea seed cake extract on Chamaecyparis obtusifolia in Chamaecyparis obtusifolia extract. Furthermore, the plant anti-dandruff composition prepared in the present application has a green and environmentally friendly extraction method, does not introduce any toxic solvents or difficult-to-handle acid-base reagents, greatly improves the safety of the composition, and through the reasonable compounding of two plant extracts, can effectively control dandruff at a relatively small dosage, and is scalp-friendly, does not cause irritation to the scalp, and is not easy to produce drug resistance. It can be widely used in the preparation of hair care products.

[0190] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A natural plant anti-dandruff composition, characterized in that, Its components include cypress extract and camellia seed cake extract; wherein, based on the total mass of the natural plant anti-dandruff composition being 100%, the content of the cypress extract is 10-90%, and the content of the camellia seed cake extract is 10-90%.

2. The natural plant anti-dandruff composition according to claim 1, characterized in that: The preparation method of the camellia seed cake extract comprises the following steps: S1: drying the tea seed meal left after oil extraction, and then crushing and sieving; S2: Take 100g of tea seed meal crushed sample, add ethanol solution, soak and extract for 20-40min, then perform ultrasonic assisted extraction for 30-60min, filter, extract 2-4 times, combine the filtrate, and concentrate under reduced pressure; S3: Freeze the concentrated sample, separate the emulsion layer, perform HP-20 macroporous resin column chromatography on the supernatant, fully elute with water, then elute with ethanol aqueous solution, and finally freeze-dry the eluate to obtain the camellia seed cake extract.

3. The natural plant anti-dandruff composition according to claim 2, characterized in that: The solid-liquid ratio of the crushed tea seed meal sample to the ethanol solution is 1g:5-25ml, the concentration of the ethanol solution is 30%-80%, and the temperature of the soaking extraction and the ultrasound-assisted extraction is 40-70°C; And / or, the particle size of the crushed tea seed meal sample is 100-200 mesh.

4. The natural plant anti-dandruff composition according to claim 1, characterized in that: The preparation method of the cypress extract comprises the following steps: S1: Dry the discarded cypress wood and sawdust after wood processing, crush and sieve; S2: Take 100g of crushed sample of Chamaecyparis obtusifolia, add anhydrous ethanol, seal and soak for 10-15h, ultrasonically treat for 40-50min, reflux extraction 2-4 times, and concentrate under reduced pressure to recover ethanol to obtain Chamaecyparis obtusifolia extract.

5. The natural plant anti-dandruff composition according to claim 4, characterized in that: The solid-liquid ratio of the crushed cypress sample and the anhydrous ethanol is 1g:5-25ml, the temperature of the reflux extraction is 40-70°C, and the extraction time is 10-60min; And / or, the particle size of the crushed cypress sample is 100-200 mesh.

6. A method for preparing a natural plant anti-dandruff composition, characterized in that: The method comprises mixing the components of the natural plant anti-dandruff composition according to any one of claims 1 to 5.

7. Use of the natural plant anti-dandruff composition according to any one of claims 1 to 5 in the preparation of hair care products; Preferably, the hair care product comprises one or more of shampoo, conditioner, hair mask and scalp essence.

8. An anti-dandruff shampoo, characterized in that: It comprises the natural plant anti-dandruff composition according to any one of claims 1 to 5; preferably, based on the total mass of the anti-dandruff shampoo being 100%, the content of the natural plant anti-dandruff composition is 0.1-10%.

9. The anti-dandruff shampoo according to claim 8, characterized in that The anti-dandruff shampoo further comprises one or a combination of two or more of a surfactant, a hair conditioner, a preservative, a pH adjuster, a fragrance, a thickener and a solvent water; Preferably, the surfactant is selected from sodium laureth sulfate and cocamidopropyl betaine; Preferably, the hair conditioning agent is selected from guar hydroxypropyltrimonium chloride; Preferably, the preservative is selected from phenoxyethanol and sodium benzoate; Preferably, the pH adjuster is selected from citric acid; Preferably, the thickener is selected from cocamide MEA and sodium chloride.

10. The anti-dandruff shampoo according to claim 9, characterized in that The preparation method of the anti-dandruff shampoo comprises the following steps: S1: Add water into an emulsifier and heat to 75-85°C, add sodium laureth sulfate and cocamidopropyl betaine, and stir until completely dissolved; S2: Add guar hydroxypropyltrimethylammonium chloride and cocamide MEA pre-dispersed in cold water, and stir at the temperature for 20-40 minutes until the mixture is uniform; S3: Cool down to 40-50℃, add sodium benzoate, phenoxyethanol, citric acid and essence, and stir evenly; S4: adding the natural plant anti-dandruff composition and stirring evenly; S5: Add sodium chloride to adjust the viscosity, stir until the material is uniform and then filter out the material.

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