Application of scenedesmus in amorphophallus bulbifer planting

Abstract

The invention discloses application of scenedesmus in amorphophallus bulbifer planting, and belongs to the technical field of amorphophallus bulbifer planting. In the planting process of the amorphophallus bulbifer, scenedesmus liquid with the biomass of 3-4 g / L is applied once a week, the application amount is 1.4-1.5 ml / m < 2 >, growth of the amorphophallus bulbifer can be promoted, and absorption of cadmium by the amorphophallus bulbifer can be reduced. The method is efficient and easy to operate, and a new technical method is provided for promoting growth of the amorphophallus bulbifer and inhibiting absorption of cadmium by the amorphophallus bulbifer.

Description

Technical Field

[0001] The invention relates to application of Scenedesmus in planting konjac, and belongs to the technical field of konjac planting. Background Art

[0002] Heavy metal pollutants are gradually increasing in today's society. These pollutants are discharged into the soil system, destroying the basic functions of the soil, affecting soil health and arable land quality, and leading to ecological and environmental risks. Among them, cadmium pollution is one of the most serious and typical heavy metal pollution in soil.

[0003] Konjac (Amorphophallus spp.) is a precious crop of the genus Amorphophallus in the family Araceae. It is native to Southeast Asia. Konjac is famous for its excellent disease resistance and high content of glucomannan (KGM). KGM has shown broad application prospects in many industries such as food, pharmaceuticals, packaging, construction, fillings and chemical sealing, and its future market potential is huge. However, heavy metal cadmium in the soil will affect the growth and development of konjac, and the cadmium in the soil will be absorbed by konjac, which will affect human health.

[0004] Therefore, it is urgent to find a method to inhibit konjac's absorption of cadmium while improving its growth. Summary of the invention

[0005] In order to improve the growth of pearly konjac in cadmium-containing soil, the present invention provides an application of Scenedesmus in the cultivation of pearly konjac: Scenedesmus algae liquid is applied during the cultivation process of pearly konjac.

[0006] Preferably, the amount of Scenedesmus solution applied is 1.4 to 1.5 L / m 2 The application frequency is: 1 to 3 times every 7 days.

[0007] Preferably, the biomass of Scenedesmus in the Scenedesmus algae liquid is 3-4 g / L.

[0008] Preferably, the BG-11 medium is used to culture the Scenedesmus algae liquid in a column reactor, and the culture conditions are: passing an air mixture containing 1.25% carbon dioxide and a light intensity of 65 μmol m -2 s -1 , temperature 25℃.

[0009] Beneficial effects of the present invention

[0010] (1) The present invention can not only improve the growth of the konjac in cadmium-containing soil by applying Scenedesmus to the konjac, but also significantly reduce the cadmium content in the leaves of the konjac.

[0011] (2) The present invention is efficient and easy to operate, and provides a new technical method for promoting the growth of konjac and inhibiting the absorption of cadmium by konjac. DETAILED DESCRIPTION

[0012] The technical solution of the present invention is further described below by specific implementation methods. However, the following embodiments are only simple examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

[0013] Example 1

[0014] The specific steps for culturing Scenedesmus are as follows:

[0015] (1) BG-11 medium was used to culture Scenedesmus algae in a column reactor, and an air mixture containing 1.25% carbon dioxide was introduced and the light intensity was 65 μmol m -2 s -1 , temperature 25℃

[0016] (2) After culturing for 7 days, a Scenedesmus algae liquid was obtained, in which the biomass of Scenedesmus was 3.5 g / L, and the liquid was set aside.

[0017] Example 2

[0018] Optimization of the application rate of Scenedesmus

[0019] In order to explore the effect of the amount of Scenedesmus applied on the growth of Amorphophallus konjac, this example first conducted 9 groups of experiments, namely: a blank control group (CK) without the application of Scenedesmus; an application of 1 L / m 2 Experimental group 1 of Scenedesmus: 1.1 L / m 2 Experimental group 2 of Scenedesmus: 1.2 L / m 2 Experimental group 3 of Scenedesmus: 1.3 L / m 2 Experimental group 4 of Scenedesmus: 1.4 L / m 2 Experimental group 5 of Scenedesmus: 1.5 L / m 2 Experimental group 6 of Scenedesmus: 1.6 L / m 2 Experimental group 7 of Scenedesmus: 1.7 L / m 2 Experimental group 8 of Scenedesmus; the specific steps are as follows:

[0020] The seeds of Amorphophallus konjac were cultivated to the seedling stage, and the seedlings with the same growth condition were selected and divided into 9 groups, with 8 plants in each group. Each group needed to be irrigated with tap water once every 7 days, with an irrigation volume of 2.8 L / m 2 , Experimental groups 1 to 8 needed to be irrigated with Scenedesmus every 7 days, and the konjac of all treatment groups needed to be cultivated in a greenhouse environment at 25°C. After 105 days of cultivation, the agronomic traits of konjac were tested. The results showed that when the application rate of Scenedesmus was greater than 1.5L / m2 The growth of Amorphophallus konjac was poor, so it was initially confirmed that the application rate of Scenedesmus was 1-1.5 L / m 2 .

[0021] In order to further explore how much Scenedesmus can inhibit the absorption of cadmium by Amorphophallus konjac, this example conducted 7 groups of experiments, including a blank control group (CK) without Scenedesmus; a control group (CK) with 1 L / m 2 Experimental group 1 of Scenedesmus: 1.1 L / m 2 Experimental group 2 of Scenedesmus: 1.2 L / m 2 Experimental group 3 of Scenedesmus: 1.3 L / m 2 Experimental group 4 of Scenedesmus: 1.4 L / m 2 Experimental group 5 of Scenedesmus: 1.5 L / m 2 Experimental group 6 of Scenedesmus; the specific steps are as follows:

[0022] The seeds of Amorphophallus konjac were cultivated to the seedling stage, and the seedlings with the same growth condition were selected and divided into 7 groups, with 8 plants in each group. Each group needed to be irrigated with tap water once every 7 days, with an irrigation volume of 2.8 L / m 2 Each group was also stressed with a 0.5 μmol / ml cadmium solution every 7 days, and the dosage of the cadmium solution was 1.5 L / m 2 , Experimental groups 1 to 6 needed to be irrigated with Scenedesmus once a week, and the konjac of all treatment groups needed to be cultivated in a greenhouse environment at 25°C. After 105 days of cultivation, the agronomic traits and cadmium content of konjac were tested. It was found that when the application amount of Scenedesmus was less than 1.4L / m 2 The application of Scenedesmus could not inhibit the absorption of cadmium by Amorphophallus konjac.

[0023] After the experiment in Example 1, it was determined that the application rate of Scenedesmus was 1.4-1.5 L / m 2 .

[0024] Example 3

[0025] In this embodiment, a total of 4 groups of experiments were conducted, namely: a blank control group (CK) without the application of Scenedesmus and without cadmium stress; an experimental group 1 (CK+algae) with the application of Scenedesmus and without cadmium stress; an experimental group 2 (Cd+algae) with the application of Scenedesmus and cadmium stress; an experimental group 3 (Cd) without the application of Scenedesmus and only with cadmium stress. The specific steps are as follows:

[0026] The seeds of Amorphophallus konjac were cultured to the seedling stage, and the seedlings with the same growth conditions were selected and divided into 4 groups, with 8 plants in each group. Among them, the first group of Amorphophallus konjac was cultured only with tap water, and the irrigation volume of tap water was 2.9L / m 2The irrigation frequency was once every 7 days; this group was the CK group; the second group of Amorphophallus konjac was irrigated with tap water like the first group, and also needed to be irrigated with Scenedesmus algae solution at a dosage of 1.5 L / m 2 The application frequency was once every 7 days. This group was the CK+algae group. In addition to being irrigated with tap water like the first group, the third group of pearly konjac also needed to be irrigated with Scenedesmus algae solution and cadmium solution at the same time. The application rate of Scenedesmus algae solution was 1.5L / m 2 The application frequency was once every 7 days; the molar concentration of the cadmium solution was 0.5 μmol / ml, and the application volume was 1.5 L / m 2 The application frequency was once every 7 days, and this group was Cd+algae. In addition to being perfused with tap water like the first group, the third group needed to be applied with cadmium solution, the molar concentration of the cadmium solution was 0.5 μmol / ml, and the application amount was 1.5 L / m 2 The application frequency was once every 7 days, and this group was the Cd group.

[0027] The konjac of all treatment groups were cultivated in a greenhouse environment at 25°C. After 105 days of cultivation, the agronomic traits of the konjac and the cadmium content in the leaves of the konjac were tested.

[0028] Agronomic traits include plant height, root length, leaf width, fresh weight, stem girth and number of compound leaves. The test results of each group are shown in Table 1. It can be seen from Table 1 that by comparing CK with CK+algae, it can be found that the agronomic traits of pearly konjac can be effectively improved by applying Scenedesmus algae solution. Compared with not applying Scenedesmus algae solution, the plant height, leaf width, fresh weight, stem girth and leaf number of pearly konjac with Scenedesmus algae solution increased by 3.32%, 5.67%, 25.05%, 2.67% and 17.78%, respectively; by comparing the CK group, Cd+algae group and Cd group, it was found that the presence of cadmium in the soil would inhibit the growth of pearly konjac. The plant height, root length and leaf width in the Cd group were relatively higher. The CK group significantly decreased by 25.65%, 46.84% and 83.94%, and the fresh weight, stem girth and number of compound leaves decreased by 50.59%, 16.16% and 9.76% respectively. Compared with the Cd group, it was found that the application of Scenedesmus algae solution can alleviate the inhibitory effect of cadmium on the growth of Amorphophallus konjac. Under cadmium stress, the plant height, root length, leaf width, fresh weight, stem girth and number of compound leaves of Amorphophallus konjac increased by 12.64%, 13.52%, 36.79%, 27.76%, 5.18% and 36.59% respectively by applying Scenedesmus algae solution. It can be seen that Scenedesmus algae solution can alleviate the impact of cadmium stress on plants.

[0029] Table 1 Statistical results of agronomic traits of Amorphophallus konjac under different treatments

[0030] deal with Plant height(cm) Root length (cm) Leaf width(cm) Fresh weight(g) Stem circumference (cm) Number of compound leaves CK 30.44 7.88 2.86 4.46 4.19 5.63 CK+Algae 31.45 6.64 3.04 5.58 4.30 6.64 Cd 24.23 5.36 1.56 2.96 3.61 5.13 Cd+algae 27.29 6.01 2.14 3.78 3.80 7

[0031] The cadmium content in the leaves of each group of pearly konjac is shown in Table 2. It can be seen from the table that the application of Scenedesmus algae solution can reduce the cadmium content in the leaves of pearly konjac. Compared with the CK group, the cadmium content in the leaves of pearly konjac in the CK+algae group was reduced by 61.29% times, and compared with the Cd group, the cadmium content in the Cd+algae group was reduced by 47.43%. It can be seen that the application of Scenedesmus algae solution can effectively inhibit the absorption of heavy metal cadmium by pearly konjac.

[0032] Table 2 Cadmium content in leaves of konjac bulbil in different treatment groups

[0033] Group Cadmium content (mg / kg) CK 0.31 CK+Algae 0.12 Cd 11.3 Cd+algae 5.94

[0034] Example 4

[0035] This example was conducted in the Kunming University Amorphophallus konjac greenhouse.

[0036] First, two plots of konjac with similar growth were selected for the experiment. One plot was irrigated with tap water only, which was the CK group. The other plot was irrigated with tap water and the Scenedesmus algae solution prepared in the example was applied at a rate of 1.5 L / m 2 , the application frequency was once every 7 days, and this group was the CK+algae group. After 180 days of cultivation, the plant height, leaf width, fresh weight, petiole and leaf number of the pearly konjac in each plot, as well as the cadmium content in the leaves of each pearly konjac were measured. Finally, the average plant height, average leaf width, average fresh weight, petiole (base diameter) and average number of compound leaves of each group, as well as the average cadmium content in the leaves of each pearly konjac were calculated. The results are shown in Table 3. It can be seen from Table 3 that the Scenedesmus algae solution can not only improve the growth of the pearly konjac, but also effectively inhibit the absorption of cadmium by the pearly konjac.

[0037] Table 3 Agronomic traits and cadmium content of two groups of different treatments of Amorphophallus konjac

[0038]

Claims

1. A Scenedesmus Acutodesmus sp. ) in the cultivation of bulbil konjac, characterized in that: Apply Scenedesmus algae solution during the cultivation of Amorphophallus konjac.

2. The use of the Scenedesmus according to claim 1 in the cultivation of Amorphophallus konjac, characterized in that: The application rate of Scenedesmus algae solution is 1.4~1.5L / m 2 , application frequency is: 1 to 3 times every 7 days.

3. The use of the Scenedesmus according to claim 1 in the cultivation of Amorphophallus konjac, characterized in that: The biomass of Scenedesmus in the Scenedesmus algae solution is 3~4g / L.

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