Deodorizing and antibacterial emulsion containing compound plant essential oil for vehicle and preparation method thereof

Abstract

The present invention belongs to the technical field of natural antibacterial agents, and specifically relates to an antibacterial emulsion for vehicles containing compound plant essential oils for deodorization and a preparation method thereof. The antibacterial emulsion is composed, by mass percentage, of: 1%-2% atractylodes lancea essential oil, 1%-5% chuanxiong essential oil, 1%-3% elsholtzia essential oil, 1%-5% tangerine peel essential oil, 1%-5% star anise essential oil, 20%-30% chuanxiong aromatic water, and 20%-30% elsholtz aromatic water. To these components, 4%-10% of an emulsifier and 10%-20% of a dispersant are added to form a uniform antibacterial emulsion. The present invention utilizes the volatility of the essential oils to allow them to evaporate freely within the vehicle, inhibiting bacteria while absorbing odors, thereby providing a green, fresh air environment within the vehicle.

Description

Technical Field

[0001] The invention belongs to the technical field of natural antibacterial agents, and particularly relates to a vehicle-use deodorizing and antibacterial emulsion containing compound plant essential oil and a preparation method thereof. Background Art

[0002] With the development of the world, the number of cars is increasing. The enclosed environment inside cars provides a breeding ground for bacteria, especially Staphylococcus aureus. Over time, the odor and harmful gases emitted by car interiors and other items will have adverse effects on the human body. Currently, most car interior cleaning and disinfection methods use chemical agents, which can cause secondary contamination during cleaning, creating a negative experience for occupants who spend extended periods in such an environment.

[0003] Natural plants have a long history of medicinal use in China, and many essential oils have been clinically demonstrated to possess antibacterial activity. These essential oils are popular due to their low toxicity, environmental friendliness, and environmentally friendly preparation. Some essential oils can also absorb foreign matter and harmful gases, contributing to freshening the air.

[0004] However, the current essential oils on the market have also exposed some drawbacks in practical applications. For example, the high volatility of pure essential oil products results in unstable or even weak antibacterial effects during use. Furthermore, the low water solubility and complex composition of essential oils themselves lead to poor compatibility of compounded essential oils, making it difficult to prepare suitable dosage forms. Summary of the Invention

[0005] The purpose of the present invention is to solve the current problems of bacterial contamination and odor in automobiles, and to provide a vehicle-use odor-removing and antibacterial emulsion containing compound plant essential oil and an application method thereof.

[0006] In order to achieve the above object, the present invention provides a vehicle-use deodorizing and antibacterial emulsion containing compound plant essential oils, the raw material composition of which is calculated by weight percentage: 1%-2% of atractylodes lancea essential oil, 1%-5% of chuanxiong essential oil, 1%-3% of elsholtzia essential oil, 1%-5% of tangerine peel essential oil, 1%-5% of star anise essential oil, 20%-30% of chuanxiong aromatic water, 20%-30% of elsholtz aromatic water, 4%-10% of emulsifier, and 10%-20% of dispersant.

[0007] Among them, the extraction methods of atractylodes lancea essential oil, tangerine peel essential oil and star anise essential oil are as follows: atractylodes lancea, tangerine peel and star anise are crushed respectively, placed in a round-bottom flask, added with deionized water, pretreated with ultrasound for 10 minutes, heated to boiling, and volatile oils are extracted by steam distillation. The upper oily liquid in the volatile oil extractor is collected to obtain the essential oils.

[0008] The extraction methods of Chuanxiong essential oil, Elsholtzia ciliata essential oil, Chuanxiong aromatic water and Elsholtzia ciliata aromatic water are as follows: respectively crushing Chuanxiong and Elsholtzia ciliata, placing them in a round-bottom flask, adding deionized water, pre-treating them by ultrasonication for 10 minutes, heating them to boiling, extracting them by steam distillation, collecting the upper oily liquid in the volatile oil extractor to obtain the essential oil, and collecting the water liquid in the lower layer of the volatile oil extractor to obtain the aromatic water.

[0009] The mass volume ratio of solid to liquid in the above extraction process is 1:8 to 1:12; and the extraction time is 2 to 3.5 hours.

[0010] The emulsifier is Tween 80 and the dispersant is olive oil.

[0011] The preparation method of the deodorizing and antibacterial emulsion containing compound plant essential oil for automobiles is as follows:

[0012] (1) According to the above mass percentages, Atractylodes lancea essential oil, Chuanxiong essential oil, Elsholtzia ciliata essential oil, Tangerine peel essential oil, Star anise essential oil and emulsifier Tween 80 were added to the dispersant olive oil to obtain an oil phase after being completely dispersed;

[0013] (2) According to the above mass percentages, a mixture of Chuanxiong aromatic water and Elsholtzia aromatic water is taken as the aqueous phase;

[0014] (3) At a water bath temperature of 56° C. and a stirring speed of 400 rpm, the oil phase is added to the water phase while stirring to uniformly mix the oil phase and the water phase to obtain a uniform plant essential oil emulsion.

[0015] Beneficial effects

[0016] The emulsion of the present invention is used in automobiles, taking into account both its volatility and its stability. Therefore, different natural plant essential oils are rationally combined to prepare a compound emulsion. The volatility of the essential oils is utilized to allow them to evaporate freely in the vehicle, inhibiting bacteria while absorbing odors. Under the premise of maintaining the advantages of being green and safe, it provides a fresh air environment in the vehicle.

[0017] During the preparation of the emulsion, aromatic water produced during the essential oil extraction process is selected as the aqueous phase of the emulsion, thereby reducing the instability of the compound essential oil. In addition, in view of the high volatility of the essential oil, the present invention designs an application form of the compound essential oil emulsion, which, while utilizing the volatility of the essential oil, also enables the effective ingredients in the essential oil to be slowly released, thereby avoiding the unstable efficacy caused by its high volatility. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is the inhibition zone result diagram of the emulsion against Staphylococcus aureus in Example 1.

[0019] Figure 2This is the inhibition zone result of the emulsion against Staphylococcus aureus when the extraction time of each essential oil is 3 hours in Example 3.

[0020] Figure 3 This is the inhibition zone result diagram of the emulsion against Staphylococcus aureus in Example 5.

[0021] Figure 4 This is the inhibition zone result diagram of the emulsion against Staphylococcus aureus in Comparative Example 1.

[0022] Figure 5 This is the inhibition zone result diagram of the emulsion against Staphylococcus aureus in Comparative Example 2.

[0023] Figure 6 This is the inhibition zone result diagram of the emulsion against Staphylococcus aureus in Comparative Example 3.

[0024] Figure 7 Total ion current chromatogram of Atractylodes lancea essential oil detected by gas chromatography-mass spectrometry.

[0025] Figure 8 Total ion current chromatogram of Chuanxiong essential oil detected by gas chromatography-mass spectrometry. DETAILED DESCRIPTION

[0026] The present invention will be further described below with reference to the examples, but is not limited thereto.

[0027] Example 1

[0028] 1. Extraction of various plant essential oils and aromatic waters

[0029] (1) 100 g each of Atractylodes lancea, dried tangerine peel, and star anise were respectively ground into coarse powder, passed through a 20-mesh sieve, placed in a round-bottom flask, and deionized water with a solid-liquid ratio of 1:12 (W / V) was added. Ultrasonic pretreatment was performed at 30°C for 10 minutes. The round-bottom flask was removed, and a volatile oil extractor was placed on top of it. The volatile oil was heated to boiling, and steam distillation was used to extract the volatile oil of Atractylodes lancea, volatile oil of dried tangerine peel, and volatile oil of star anise. After extraction for 2.5 hours, the oily liquid in the volatile oil extractor was collected to obtain the essential oil of Atractylodes lancea, essential oil of dried tangerine peel, and essential oil of star anise, respectively.

[0030] (2) 100 g each of Chuanxiong and Elsholtzia ciliata were respectively ground into coarse powder, passed through a 20-mesh sieve, placed in a round-bottom flask, and deionized water with a solid-liquid ratio of 1:12 (W / V) was added. The mixture was ultrasonically pretreated at 30°C for 10 minutes. The round-bottom flask was removed, and a volatile oil extractor was placed on top of it. The mixture was heated to boiling, and the volatile oil of Atractylodes lancea and Elsholtzia ciliata was extracted by steam distillation. After extraction for 2.5 hours, the upper layer of the oily liquid in the volatile oil extractor was collected to obtain Chuanxiong essential oil and Elsholtzia ciliata essential oil, respectively. The water in the middle and lower layers of the volatile oil extractor was collected to obtain Chuanxiong aromatic water and Elsholtzia ciliata aromatic water, respectively.

[0031] 2. Preparation method of plant essential oil emulsion

[0032] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 5%, Elsholtzia ciliata essential oil 3%, Tangerine peel essential oil 5%, Star anise essential oil 5%, Chuanxiong aromatic water 30%, Elsholtzia ciliata aromatic water 25%, Tween 80 emulsifier 8%, Olive oil 18%.

[0033] (1) According to the above mass percentages, the essential oils of Atractylodes lancea, Ligusticum chuanxiong, Elsholtzia ciliata, Tangerine peel, and Star anise were added to the olive oil and the emulsifier Tween 80 to completely disperse them to obtain the oil phase of the emulsion.

[0034] (2) According to the above mass percentages, the Ligusticum chuanxiong aromatic water and the Elsholtzia aromatic water were mixed evenly to obtain the aqueous phase of the emulsion.

[0035] (3) Add the oil phase to the water phase while stirring at a water bath temperature of 56° C. and a stirring speed of 400 rpm to uniformly mix the oil phase and the water phase to obtain a uniform plant essential oil emulsion.

[0036] Example 2

[0037] The preparation method of this embodiment is the same as that of Example 1, except that the solid-liquid ratio of the solvent used in extracting the Atractylodes lancea essential oil by steam distillation is different. The volume of the Atractylodes lancea essential oil obtained under different extraction conditions is shown in Table 1.

[0038] Table 1

[0039] Solid-liquid ratio of different extraction solvents (W / V) Essential oil volume (mL) Solid-liquid ratio 1:7 1.43 Solid-liquid ratio 1:8 1.51 Solid-liquid ratio 1:9 1.62 Solid-liquid ratio 1:10 1.66 Solid-liquid ratio 1:11 1.73 Solid-liquid ratio 1:12 1.91 Solid-liquid ratio 1:13 1.97 Solid-liquid ratio 1:14 1.98

[0040] As can be seen from Table 1, when solvents with different solid-liquid ratios are used to extract essential oils, the solid-liquid ratios are different, and the volumes of essential oils extracted are also different. When the solid-liquid ratio (W / V) is 1:7, the volume of the Atractylodes lancea essential oil obtained is 1.43mL. As the amount of extraction solvent increases, the volume of essential oil extracted also increases continuously. When the solid-liquid ratio (W / V) is 1:12, the volume of the Atractylodes lancea essential oil reaches 1.91mL. When the amount of solvent is further increased and the solid-liquid ratio (W / V) of 1:13 and 1:14 is used for extraction, the volumes of essential oil obtained are 1.97mL and 1.98mL respectively. It can be seen that when the volume of the extraction solvent exceeds 12 times the amount of medicinal materials, the amount of Atractylodes lancea essential oil obtained does not increase much, but the increase in solvent usage will bring about a series of cumbersome operations. Therefore, the present invention optimizes the solid-liquid ratio (W / V) of the solvent used in the extraction of Atractylodes lancea essential oil to 1:12. Similarly, the optimized solid-liquid ratio (W / V) of the solvent used for the extraction of several other essential oils was also set at 1:12.

[0041] Example 3

[0042] The preparation method of this embodiment is the same as that of Example 1, except that the extraction time used for extracting the Atractylodes lancea essential oil by steam distillation is different. The specific extraction conditions are different, and the volume of the Atractylodes lancea essential oil obtained is shown in Table 2.

[0043] Table 2

[0044]

[0045] As shown in Table 2, along with the increase of extraction time, the essential oil volume obtained by extraction also continuously increases, and when the extraction time is 3h, the volume of atractylodes lancea essential oil reaches 1.92mL.And continue to increase the extraction time, when reaching 3.5h, the atractylodes lancea essential oil volume obtained is respectively 2.03mL.As can be seen, when the extraction time exceeds 3h, the atractylodes lancea essential oil amount increases and is not much, considering that the extraction time is longer and has a certain impact on the stability of volatile oil components, and the time cost of extraction, the present invention optimizes the atractylodes lancea essential oil extraction time to 3h.Similarly, other several essential oil extraction times are also optimized to 3h.

[0046] Example 4

[0047] The preparation method of this embodiment is the same as that of embodiment 1, except that in the second step of the plant essential oil emulsion preparation method, the raw materials are composed of:

[0048] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 1%, Elsholtzia ciliata essential oil 3%, Tangerine peel essential oil 3%, Star anise essential oil 3%, Chuanxiong aromatic water 30%, Elsholtzia ciliata aromatic water 30%, Tween 80 emulsifier 10%, Olive oil 19%.

[0049] Example 5

[0050] The preparation method of this embodiment is the same as that of embodiment 1, except that in the second step of the plant essential oil emulsion preparation method, the raw materials are composed of:

[0051] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 5%, Elsholtzia ciliata essential oil 3%, Tangerine peel essential oil 5%, Star anise essential oil 5%, Chuanxiong aromatic water 30%, Elsholtzia ciliata aromatic water 27%, Tween 80 emulsifier 4%, Olive oil 20%.

[0052] Comparative Example 1

[0053] The preparation method of this embodiment is the same as that of Example 1, except that in the first step of the plant essential oil extraction method, the medicinal raw materials are not subjected to ultrasonic pretreatment, but are directly extracted by steam distillation.

[0054] Comparative Example 2

[0055] The preparation method of this embodiment is the same as that of embodiment 1, except that in the second step of the plant essential oil emulsion preparation method, distilled water is used instead of Chuanxiong aromatic water and Elsholtzia aromatic water, that is, the raw material composition is:

[0056] Atractylodes lancea essential oil 1%, Chuanxiong essential oil 5%, Elsholtzia ciliata essential oil 3%, Tangerine peel essential oil 5%, Star anise essential oil 5%, distilled water 55%, Tween 80 emulsifier 8%, olive oil 18%.

[0057] Comparative Example 3

[0058] The preparation method of this embodiment is the same as that of Example 1, except that this embodiment does not contain any plant essential oil extracts, but only contains Chuanxiong aromatic water and Elsholtzia aromatic water, that is, the raw material composition is: 30% Chuanxiong aromatic water, 25% Elsholtzia aromatic water, 8% Tween 80 emulsifier, and 37% olive oil.

[0059] Comparative Example 4

[0060] The preparation method is the same as that in Example 1, except that this example does not contain Atractylodes lancea essential oil and Tangerine peel essential oil extract, that is, the raw material composition is: Chuanxiong essential oil 5%, Elsholtzia essential oil 3%, Star anise essential oil 5%, Chuanxiong aromatic water 30%, Elsholtz aromatic water 30%, Tween 80 emulsifier 8%, olive oil 19%.

[0061] Antibacterial experiment: inhibition zone experiment

[0062] The experiment was performed according to the standards of the Clinical Laboratory Standards Institute (CLSI). 15 mL of melted nutrient agar medium was added to a sterile plate and allowed to solidify. 10 mL of the nutrient agar medium was taken up with a pipette. 6 Place 100 μL of a CFU / mL Staphylococcus aureus suspension on a plate and spread evenly with a coating rod. Separately, soak a 6mm diameter drug-sensitive paper disc in the test sample solution for 30 minutes, remove, and air-dry in a sterile environment. Place the drug-sensitive paper discs for the test sample and a blank control group (sterile water) on the plate, centering the control sample and placing the test sample on the periphery. Incubate in a 37°C incubator for 24 hours and observe the formation of inhibition zones on the plate.

[0063] The sensitivity criteria for determining the antibacterial effect are as follows: if the diameter of the inhibition zone is <10mm, it is considered as low sensitivity and ineffective (-); if the diameter is 10mm ≤ and <15mm, it is considered as medium sensitivity and effective (+); and if the diameter is 15mm ≤ and <20mm, it is considered as high sensitivity and effective (++). Each test sample must be repeated three times. If there is an inhibitory effect in all three tests and no inhibition zone is produced in the negative control group, the test result is considered qualified.

[0064] The antibacterial effects of Examples 1-5 and Comparative Examples 1-4 (emulsions) on Staphylococcus aureus are shown in Table 3 and the attached table. Figure 1-6 shown.

[0065] Table 3

[0066] Diameter of inhibition zone (mm) Result determination Example 1 18.01 ++ Example 2 (solvent solid-liquid ratio 1:13) 17.82 ++ Example 3 (essential oil extraction time is 3h) 17.98 ++ Example 4 13.04 + Example 5 15.13 ++ Comparative Example 1 14.22 + Comparative Example 2 13.74 + Comparative Example 3 6.25 - Comparative Example 4 11.35 +

[0067] Analysis experiment of volatile components of Atractylodes lancea essential oil and Ligusticum chuanxiong essential oil:

[0068] GC-MS (gas chromatography-mass spectrometry) was used to detect the volatile components of the essential oils of Atractylodes lancea and Ligusticum chuanxiong.

[0069] Chromatographic conditions: instrument model Agilent 7890 / 5975C, chromatographic column: HP-5MS (30 m × 250 μm × 0.25 μ), diluent: chromatographic-grade dichloromethane; temperature program: column temperature: 100°C (hold for 0 min), then increase to 220°C at a rate of 3°C / min and hold for 5 min; carrier gas: high-purity He; column flow rate: 1.2 ml / min, split ratio: 50:1.

[0070] Mass spectrometry conditions: EI ion source; ion source temperature 230°C; MS quadrupole temperature 150°C; injection port temperature 220°C; scanning range 30-550 amu.

[0071] The total ion current chromatogram of Atractylodes lancea essential oil is attached. Figure 7 , of which the content of β-eudesmol is relatively high, reaching 39.85%; the total ion current chromatogram of Chuanxiong essential oil is attached. Figure 8 , among which the content of ligustilide is relatively high, reaching 66.93%.

[0072] Investigation into improvements in in-car air freshness:

[0073] Table 4

[0074]

[0075] Cars with similar interior air quality were selected. 20 mL of each of Examples 1-5 and Comparative Examples 1-4 (emulsions) were applied to the sealed cars and allowed to evaporate freely. Eleven people were invited to observe and experience the freshness of the air inside the cars 2 and 12 hours after use. A blank group, without any product, was also selected as a blank control. The improvement in car air freshness achieved by each sample group is shown in Table 4. As can be seen from the table, Examples 1-5 showed some improvement in the interior air quality after 2 hours of use, and no irritation was observed over time.

Claims

1. A deodorizing and antibacterial emulsion for vehicles containing compound plant essential oils, characterized in that: The antibacterial emulsion is composed of the following components by weight: 1%-2% of atractylodes lancea essential oil, 1%-5% of chuanxiong essential oil, 1%-3% of elsholtzia essential oil, 1%-5% of tangerine peel essential oil, 1%-5% of star anise essential oil, 20%-30% of chuanxiong aromatic water, 20%-30% of elsholtz aromatic water, 4%-10% of emulsifier, and 10%-20% of dispersant; The extraction method of the essential oil of Atractylodes lancea, the essential oil of dried tangerine peel and the essential oil of star anise comprises: crushing the Atractylodes lancea, the dried tangerine peel and the star anise respectively, placing the crushed pieces in a round-bottom flask, adding deionized water, performing ultrasonic pretreatment for 10 minutes, heating the pieces to boiling, extracting the volatile oil by steam distillation, and collecting the upper oily liquid in the volatile oil extractor to obtain the essential oil; The extraction methods of the Chuanxiong essential oil, the Elsholtzia ciliata essential oil, the Chuanxiong aromatic water, and the Elsholtzia ciliata aromatic water are as follows: respectively crushing the Chuanxiong and the Elsholtzia ciliata, placing them in a round-bottom flask, adding deionized water, pre-treating them with ultrasound for 10 minutes, heating them to boiling, extracting them by steam distillation, collecting the upper oily liquid in the volatile oil extractor to obtain the essential oil, and collecting the water in the lower layer of the volatile oil extractor to obtain the aromatic water; The solid-liquid mass-to-volume ratio during the extraction process is 1:8-1:12; the extraction time is 2-3.5 hours; The emulsifier is Tween 80 and the dispersant is olive oil.

2. A method for preparing the deodorizing and antibacterial emulsion containing compound plant essential oil for vehicles as claimed in claim 1, characterized in that: The preparation method comprises the following steps: adding atractylodes lancea essential oil, chuanxiong essential oil, elsholtzia essential oil, tangerine peel essential oil, star anise essential oil and an emulsifier into a dispersant according to mass percentage, and completely dispersing the oil phase to obtain an oil phase; mixing chuanxiong aromatic water and elsholtz aromatic water according to mass percentage to form a water phase; and adding the oil phase into the water phase while stirring, so that the oil phase and the water phase are evenly mixed, thereby preparing an antibacterial emulsion containing the compound plant essential oil.

3. The method for preparing the deodorizing and antibacterial emulsion containing compound plant essential oil for vehicle use as claimed in claim 2, wherein: When the oil phase was added to the water phase, the water bath temperature was 56°C and the stirring speed was 400 rpm.

4. An application of the deodorizing and antibacterial emulsion containing compound plant essential oil for use in a vehicle as claimed in claim 1, characterized in that: The antibacterial emulsion is used as an emulsion for removing odor in a car, refreshing the air, and inhibiting Staphylococcus aureus.

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