A platinum tea composition with whitening and repairing effects and its application

By combining the fermented product filtrate of golden flower white tea, carob seed extract, and decarboxylated carnosine hydrochloride, the problems of the stability and single efficacy of antioxidant components of white tea in cosmetics are solved, and the whitening and repair effects of cosmetics are achieved.

CN119499149BActive Publication Date: 2025-10-31GUANGZHOU ZHONGZHUANG BEAUTY COSMETICS CO LTD +1
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Patent Information

Application Number
CN202411868844.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-18
Publication Date
2025-10-31
Estimated Expiration
2044-12-18

AI Technical Summary

Technical Problem

The current application of white tea in cosmetics suffers from problems such as poor stability of antioxidant components, limited efficacy, and inability to meet the complex needs of skin. The activity of carob seed extract is easily affected by environmental factors, and decarboxylated carnosine hydrochloride has limited effect in anti-glycation. It needs to be used in combination with other ingredients to enhance the effect.

Method used

The fermented product filtrate of golden flower white tea, carob seed extract, and decarboxylated carnosine hydrochloride are combined to form a platinum tea composition, which works synergistically to exert antioxidant and whitening repair effects, ensuring stability and effectiveness under different environmental conditions.

Benefits of technology

It achieves highly effective whitening and repairing effects in cosmetics, with comprehensive antioxidant benefits, overcoming the shortcomings of single-ingredient applications and improving the efficacy and safety of cosmetics.

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Abstract

This invention relates to the field of cosmetics, and more particularly to a whitening and repairing composition of *Tea japonica* and its application. The whitening and repairing composition of this invention comprises the following components in parts by weight: 5-15 parts of *Tea japonica* fermentation product filtrate, 1-6 parts of carob seed extract, and 1-2 parts of decarboxylated carnosine hydrochloride. This invention combines *Tea japonica* fermentation product filtrate, carnosine seed extract, and decarboxylated carnosine hydrochloride, which have a synergistic effect, effectively enhancing the whitening, antioxidant, and repairing effects of the composition. Cosmetics prepared by adding this to cosmetics are safe, highly stable, and have good transdermal absorption, exhibiting highly effective whitening and repairing effects.
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Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more particularly to a platinum tea composition with whitening and repairing effects and its application. Background Technology

[0002] Currently, scientific research on white tea mainly focuses on physicochemical component analysis, antibacterial, antimutagenic, anticancer, and obesity prevention, while research on its in vitro antioxidant and skin anti-aging effects is relatively limited. Its application in cosmetics is largely unexplored, and its skincare value has not yet been fully realized. Although tea polyphenols have excellent skincare benefits, their poor stability limits their application concentration, preventing them from reaching their maximum potential. Relying solely on the antioxidant components of white tea may result in limited effectiveness and an inability to meet the complex needs of the skin. While carob seed extract can activate the skin's antioxidant enzyme system, its active ingredients are susceptible to loss of activity due to factors such as light, temperature, and pH. Suitable formulations and technologies need to be developed to ensure its stability and fully realize its antioxidant effects, meeting the needs of skincare products under different environmental conditions. The effect of decarboxylated carnosine hydrochloride in combating glycation alone may not comprehensively improve the overall skin condition; it needs to be used in combination with other ingredients with different effects to achieve better skincare results. How to further optimize its application in skincare products, improve its anti-glycation effects, and ensure safety are urgent technical challenges that need to be addressed. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a platinum tea composition with highly effective whitening and repairing effects.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0005] In a first aspect, the present invention provides a platinum tea composition comprising the following components in parts by weight: 5-15 parts of fermented product filtrate of golden flower white tea, 1-6 parts of carob seed extract, and 1-2 parts of decarboxylated carnosine hydrochloride.

[0006] Aged white tea, also known as platinum tea, refers to white tea that has been stored for many years, with "many years" meaning within a reasonable shelf life, such as 10-20 years. White tea is a lightly fermented tea. Studies have shown that white tea contains higher levels of flavonoids than other teas, making it a special and precious variety of Chinese tea. Fresh white tea leaves undergo solid-state aerobic fermentation to obtain the fermented filtrate of golden-flower white tea. The polyphenols and flavonoids in white tea have strong antioxidant activity, capable of scavenging free radicals in the body. Tea polyphenols, also known as antioxidants, are a class of polyhydroxy compounds found in tea leaves, possessing strong antioxidant and physiological activity, acting as scavengers of free radicals in the human body. One milligram of tea polyphenols has the same effect in scavenging excess free radicals harmful to the human body as 9 micrograms of superoxide dismutase (SOD), significantly higher than other similar substances. Carob seed extract can activate the skin's antioxidant enzyme system, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). These enzymes catalyze the decomposition of free radicals, reducing their concentration and protecting skin cells from oxidative damage. Decarboxylcarnosine hydrochloride inhibits glycation reactions. During glycation, reactive carbonyl compounds such as methylglyoxal (MGO) and glyoxal are produced. These compounds react with biomolecules such as proteins and nucleic acids, leading to the formation of advanced glycation end products (AGEs). Decarboxylcarnosine hydrochloride has strong nucleophilicity and can bind to reactive carbonyl compounds to form stable complexes, thereby preventing them from reacting with biomolecules and reducing AGE formation. This invention combines fermented tea filtrate, carob seed extract, and decarboxylcarnosine hydrochloride, resulting in a synergistic effect. This overcomes the shortcomings of using these three components separately in cosmetics, allowing them to exert their maximum skincare efficacy. The three components—fermentation product filtrate of golden flower white tea, carob seed extract, and decarboxylated carnosine hydrochloride—all possess strong antioxidant properties. Decarboxylated carnosine hydrochloride can inhibit glycation reactions. When combined, these three components exhibit highly effective whitening and repairing effects, providing more comprehensive antioxidant benefits and bringing new possibilities for cosmetic applications.

[0007] Preferably, the platinum tea composition comprises the following components in parts by weight: 7-15 parts of fermented white tea filtrate, 3-6 parts of carob seed extract, and 1.4-2 parts of decarboxylated carnosine hydrochloride.

[0008] More preferably, the platinum tea composition comprises the following components in parts by weight: 15 parts of fermented white tea filtrate, 5 parts of carob seed extract, and 2 parts of decarboxylated carnosine hydrochloride.

[0009] Secondly, the present invention provides the application of the above-mentioned platinum tea composition in cosmetics.

[0010] Thirdly, the present invention provides a cosmetic comprising the above-described platinum tea composition.

[0011] Preferably, the amount of the platinum tea composition added to the cosmetic is 7.00%-23.00%.

[0012] More preferably, the amount of the platinum tea composition added to the cosmetic is 22.00%.

[0013] Preferably, the dosage form of the cosmetic includes water, lotion, and cream.

[0014] Preferably, the cosmetic also includes excipients acceptable for use in daily chemical products.

[0015] Preferably, the excipients include at least one of emollients, dispersants, humectants, preservatives, thickeners, and pH adjusters.

[0016] The beneficial effects of this invention are as follows:

[0017] This invention combines fermented product filtrate of golden flower white tea, carob seed extract, and decarboxylated carnosine hydrochloride, which has a synergistic effect and can overcome the shortcomings of applying these three components separately in cosmetics, allowing the three components to exert their maximum skin care efficacy.

[0018] The three components—fermentation product filtrate of golden flower white tea, carob seed extract, and decarboxylated carnosine hydrochloride—all possess strong antioxidant properties. Decarboxylated carnosine hydrochloride can inhibit glycation reactions. When combined, these three components exhibit highly effective whitening and repairing effects, providing more comprehensive antioxidant benefits and bringing new possibilities for cosmetic applications. Attached Figure Description

[0019] Figure 1 This is a sample of the Platinum Tea composition from Example 10. Detailed Implementation

[0020] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0021] The raw materials used in this invention are sourced from:

[0022] The fermented product filtrate of golden flower white tea was purchased from Bozhou Mingqi Biotechnology Co., Ltd.; the extract of carob seeds was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd.; and the decarboxylated carnosine hydrochloride was purchased from Zhejiang Paitai Biotechnology Co., Ltd.

[0023] The appearance of the platinum tea composition sample prepared according to the present invention is shown in the figure below. Figure 1 As shown (using Example 10 as an example).

[0024] The cosmetic prepared by this invention is left to stand after being discharged from the production process, and then inspected as a semi-finished product. After passing the inspection, it is filled and packaged. Finally, the finished product is inspected to obtain the final product.

[0025] Examples 1-12:

[0026] Examples of the Platinum Tea Composition of the present invention; the weight parts of the components of the Platinum Tea Composition are shown in Table 1.

[0027] Table 1. Components (by weight) of the Platinum Tea Compositions described in each embodiment

[0028]

[0029] Comparative Examples 1-4:

[0030] Comparative Examples 1-4 are comparative examples of the platinum tea composition described in this invention; wherein Comparative Example 4 is a green tea composition.

[0031] The weight parts of the components of each comparative composition are shown in Table 2.

[0032] Table 2. Component weight parts of each comparative example composition

[0033]

[0034] The only difference between Comparative Examples 1-4 and Example 10 is that: Comparative Example 1 does not add the fermented product filtrate of golden chrysanthemum white tea; Comparative Example 2 does not add carob seed extract; Comparative Example 3 does not add decarboxylated carnosine hydrochloride; the missing components are replaced with an equal part by weight of water; and Comparative Example 4 replaces the fermented product filtrate of golden chrysanthemum white tea with an equal part by weight of green tea fermented product filtrate.

[0035] Application Examples 1-12:

[0036] Application examples of the cosmetic described in this invention; the cosmetic is specifically a face cream; the cosmetic includes a platinum tea composition.

[0037] The formulation of the cosmetic is shown in Table 3; the content of the platinum tea composition added in each application embodiment is shown in Table 4.

[0038] Table 3 shows the formulation of the cosmetic described in Application Example 1.

[0039]

[0040] Table 4 shows the content of the platinum tea composition added in each application example.

[0041]

[0042]

[0043] The preparation method of the cosmetic includes the following steps:

[0044] (1) Add appropriate amounts of water, glycerin and carbomer to the emulsification pot in sequence, stir at 800-1200 rpm, then homogenize at 2000-2500 rpm for 1-3 min, heat to 80-85℃ and keep warm for 10-15 min until completely dissolved and no gel is formed.

[0045] (2) Add caprylic / capric triglyceride, cetearyl alcohol, C10-18 fatty acid triglycerides, glyceryl stearate / PEG-100 stearate, shea butter, and polydimethylsiloxane to the oil phase pot, stir at 800-1200 rpm, heat to 80-85℃, homogenize at 800-1200 rpm for 5-8 min, and stir until completely dissolved;

[0046] (3) Cool down to 50-55℃, add the pre-dispersed butanediol and p-hydroxyacetophenone in sequence, stir at 800-1200 rpm, add polyacrylamide / C13-14 isoparaffin / lauryl alcohol polyether-7 while stirring, homogenize at 800-1200 rpm for 5-8 min, and stir until completely dissolved.

[0047] (4) Cool down to 45°C, add the pre-dissolved and dispersed aminomethylpropanol that has been heated to 50-60°C, stir at 800-1200 rpm for 3-8 minutes, then add the remaining water and stir at 800-1200 rpm until it is completely emulsified and there is no oil on the surface, then start cooling down.

[0048] (5) Add the pre-dispersed platinum tea composition and phenoxyethanol in small amounts several times, stir evenly at 600-1000 rpm, cool down to 38°C, and filter out the material (100 mesh filter bag) to obtain the cosmetic.

[0049] Application Comparative Example 1:

[0050] This invention provides a comparative example of the application of the cosmetic product described herein; the cosmetic product is specifically a face cream; the only difference between this comparative example and application example 10 is that the platinum tea composition of example 10 contained in application example 10 is replaced with an equal amount of the platinum tea composition of comparative example 1, and the remaining components are the same as in application example 10.

[0051] The preparation method of the cosmetic is the same as in the application examples.

[0052] Application Comparative Example 2:

[0053] This invention provides a comparative example of the application of the cosmetic product described herein; the cosmetic product is specifically a face cream; the only difference between this comparative example and application example 10 is that the platinum tea composition of example 10 contained in application example 10 is replaced with an equal amount of platinum tea composition of comparative example 2, and the remaining components are the same as in application example 10.

[0054] The preparation method of the cosmetic is the same as in the application examples.

[0055] Application Comparative Example 3:

[0056] This invention provides a comparative example of the application of the cosmetic product described herein; the cosmetic product is specifically a face cream; the only difference between this comparative example and application example 10 is that the platinum tea composition of example 10 contained in application example 10 is replaced with an equal amount of the platinum tea composition of comparative example 3, and the remaining components are the same as in application example 10.

[0057] The preparation method of the cosmetic is the same as in the application examples.

[0058] Application Comparative Example 4:

[0059] This invention provides a comparative example of the application of the cosmetic product described herein; the cosmetic product is specifically a face cream; the only difference between this comparative example and application example 10 is that the 22.00% platinum tea composition in application example 10 is replaced with 22.00% of the green tea composition of comparative example 4, and the remaining components are the same as in application example 10.

[0060] The preparation method of the cosmetic is the same as in the application examples.

[0061] Application Comparative Example 5:

[0062] This invention provides a comparative example of the application of the cosmetic described herein; the cosmetic is specifically a face cream; the only difference between this comparative example and application example 10 is that the platinum tea composition in application example 10 is replaced with an equal amount of water, while the other components are the same as in application example 10.

[0063] The preparation method of the cosmetic is the same as in the application examples.

[0064] Test Example 1: Safety Patch Test

[0065] Test sample: A face cream sample containing the platinum tea composition prepared in Example 10.

[0066] Participants: 9 males and 26 females, a total of 35 participants, aged 22 to 45 years, with a mean age of 26.1 ± 5.6 years, meeting the criteria for voluntary inclusion.

[0067] The spot test method is as follows:

[0068] Select qualified patch testing equipment and use the closed patch test method to apply the patch containing the test sample (cut to the size of the patch tester, approximately 50mm). 2 The test sample was placed inside the patch applicator and applied to the back of the subject with hypoallergenic adhesive tape. The sample was removed after 24 hours, and skin reactions were observed at 0.5, 24, and 48 hours after removal. A negative control (blank, no treatment) was also included. The results were recorded according to the grading standards for skin reactions in occlusive patch tests as outlined in Chapter 7 of the "Cosmetic Safety Technical Specifications" (2015 edition). The safety patch test results are shown in Table 5.

[0069] Table 5

[0070]

[0071] Human skin patch test results showed that among 35 subjects, 0 cases of adverse skin reactions occurred, indicating that the platinum tea composition prepared in this invention has high safety.

[0072] Test Example 2: Antioxidant Test (DPPH Method)

[0073] Test samples: Platinum tea compositions prepared in Examples 1-12 and Comparative Examples 1-4, and blank control.

[0074] Test Principle: The test is based on the characteristic that DPPH free radicals have a single electron, exhibit strong absorption at 517 nm, and their alcoholic solution turns purple. When a free radical scavenger is present, it pairs with the single electron in the DPPH free radical, causing the absorption to gradually disappear. The degree of fading is quantitatively related to the number of electrons accepted, thus allowing for rapid quantitative analysis using a spectrophotometer.

[0075] 1. Reagents and materials:

[0076] (1.1) Reagents:

[0077] 0.2 mM DPPH solution (1,1-diphenyl-2-trinitrophenylhydrazine, C 18 H 12 N5O6): Weigh 0.007875g of DPPH powder and dissolve it in 100mL of anhydrous ethanol to prepare a 0.2mM DPPH solution.

[0078] Vitamin C standard control solution (positive control group): Dilute VC sequentially with deionized water to 0.001 mg / mL, 0.002 mg / mL, 0.004 mg / mL, 0.006 mg / mL, 0.008 mg / mL, 0.01 mg / mL, 0.02 mg / mL and 0.5 mg / mL, and store in brown bottles for later use.

[0079] (1.2) Instruments and equipment: electronic balance (brand: Sartiorius, accurate to 0.001g), ultraviolet spectrophotometer (brand: HITACHI).

[0080] 2. Test steps:

[0081] (2.1) Test in four sample tubes, and add samples in the order shown in Table 6.

[0082] Table 6

[0083] reagents T-sample tube <![CDATA[T0 - Sample background]]> C-DPPH pipe <![CDATA[C0 - Solvent background]]> Sample solution (mL) 2 2 / / Water or 95% ethanol solvent (mL) / 2 2 2 DPPH ethanol solution (mL) 2 / 2 /

[0084] (2.2) After mixing the solutions in each tube, react at room temperature in the dark for 30 min. Zero the spectrophotometer at a wavelength of 517 nm with distilled water and record the data. (Note that the absolute difference between two independent measurements obtained under repeatability conditions should not exceed 10% of the arithmetic mean during data processing.) Calculate the clearance rate (P) according to formula (1).

[0085]

[0086] In the formula:

[0087] T—Absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH;

[0088] T0—Sample background absorbance;

[0089] C-DPPH tube absorbance value, i.e., the absorbance value of DPPH solution without sample added;

[0090] C0 — Solvent background absorbance.

[0091] 3. Experimental Results:

[0092] Table 7

[0093]

[0094]

[0095] The experimental results are shown in Table 7. Antioxidant experiments verified that the platinum tea compositions prepared in the embodiments of this invention all exhibit good free radical scavenging and antioxidant effects. Among them, the platinum tea composition of Example 10 showed the best antioxidant effect, indicating that the increased content of the fermentation product filtrate of golden flower white tea helps to synergistically enhance its effect with other components, thereby improving the antioxidant efficacy of the platinum tea composition.

[0096] Test Example 3: Test for Inhibition of Tyrosinase Activity

[0097] Test samples: Platinum tea compositions prepared in Examples 1-12 and Comparative Examples 1-4, and blank control.

[0098] Experimental Principle: Tyrosinase is the rate-limiting enzyme in the melanin synthesis pathway. It mainly affects melanin production by influencing the conversion of tyrosine to dopa and the oxidation of dopa to dopaquinone. The principle is that tyrosine or dopa is converted to dopaquinone under the action of tyrosinase. This reaction is a colorimetric reaction, and the inhibition rate of different brightening agents on tyrosinase activity is determined by colorimetric methods.

[0099] 1. Reagents and materials:

[0100] (1.1) Reagents: Tyrosinase (25kU), Levodopa, ≥98%, α-arbutin, ≥99%

[0101] (1.2) Reagent preparation:

[0102] (1.2.1) Preparation of 0.05 mol / L PBS (pH = 6.8):

[0103] Solution A: Weigh 7.099 g of disodium hydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L Na2HPO4 solution;

[0104] Solution B: Weigh 6.803 g of potassium dihydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L KH2PO4 solution;

[0105] Simply mix 50 mL of solution A and 50 mL of solution B.

[0106] (1.2.2) Tyrosinase solution: Prepare tyrosinase to 100 U / mL using PBS buffer at pH 6.8, and prepare immediately before use;

[0107] (1.2.3) Dopa solution: Weigh 0.04 g of dopa and dissolve it in 40 mL of PBS buffer (pH 6.8). Store in the dark.

[0108] (1.2.4) α-Arbutin: Dilute α-arbutin to 100 μM with PBS buffer at pH 6.8.

[0109] 2. Instruments and equipment: analytical balance (accurate to 0.0001g), 1L volumetric flask, 50mL graduated cylinder, 1000μL pipette, constant temperature water bath, UV-Vis spectrophotometer.

[0110] 3. Measurement Procedure

[0111] Prepare the test solution according to Table 8:

[0112] Add the test sample, 0.05M PBS buffer (pH 6.8), and tyrosinase solution to the test tube in sequence;

[0113] Table 8. Composition of the reaction solution (mL)

[0114]

[0115] After preparing the test solution, incubate the test tube in a 37°C water bath for 10 min; then, add 2 mL of dopa solution, react for 5 min, and measure the absorbance at 475 nm. The absolute difference between two independent measurements obtained under repeatability conditions shall not exceed 10% of the arithmetic mean. Calculate the tyrosinase inhibition rate (%) according to formula (2).

[0116] Tyrosinase inhibition rate (%) = [1-(OD C -OD D ) / (OD A -OD B )]×100%——Formula (2)

[0117] In the above formula, OD A The absorbance value of the enzyme solution control group; OD B The absorbance value of the enzyme solution control blank group; OD C The absorbance value of the sample group; OD D This is the absorbance value of the blank control in the sample group.

[0118] Table 9

[0119] Test sample Tyrosinase inhibition rate (%) Example 1 18.76 Example 2 20.45 Example 3 21.21 Example 4 23.00 Example 5 21.76 Example 6 22.36 Example 7 23.65 Example 8 20.82 Example 9 23.22 Example 10 24.32 Example 11 23.44 Example 12 19.54 Comparative Example 1 2.87 Comparative Example 2 9.76 Comparative Example 3 10.43 Comparative Example 4 16.46 Blank control (water) -5.09

[0120] The test results are shown in Table 9. Compared with the comparative example, the platinum tea composition prepared in the embodiments of the present invention has a significant inhibitory effect on tyrosinase activity, that is, it has a good brightening and whitening effect. Among them, the platinum tea composition of Example 10 has the best effect, that is, the optimal combination ratio is: golden flower white tea fermentation product filtrate: carob seed extract: decarboxylated carnosine hydrochloride = 15: 5: 2.

[0121] Test Example 4: Repair Performance Test (Cell Migration Assay)

[0122] Experimental Principle: Cell migration ability directly reflects the cell's activity level. If cells can proliferate and migrate rapidly, they can quickly form a protective mechanism to repair damaged cells in response to external stimuli. The cell scratch (repair) method is a simple method to measure cell migration and repair ability, similar to an in vitro wound healing model. On a monolayer of adherent cells cultured in an in vitro culture dish or plate, a line is drawn in the central area of ​​cell growth using a micropipette tip or other hard object. The cells in the central part are removed, and the cells are cultured again until the experimentally set time. The cell culture plate is then removed, and it is observed whether the surrounding cells have grown (repaired) to the central scratch area, thus judging the cell's repair ability. The experiment usually requires a normal control group and an experimental group. The experimental group is a group with a certain treatment factor, drug, exogenous gene, etc. By comparing the repair ability of cells in different groups to the scratch area, the migration and repair abilities of cells in each group can be judged.

[0123] 1. Experimental materials:

[0124] (1) Equipment: Cell counter;

[0125] (2) Reagents: culture medium, trypsin, trypan blue, PBS;

[0126] (3) Consumables: Cell counting plate, Transwell plate.

[0127] 2. Prepare reagents for the test group:

[0128] (1) Negative control: DMEM culture medium containing 10% serum.

[0129] (2) Sample group: Platinum tea compositions of Examples 1-12, Comparative Examples 1-4, and blank control; Platinum tea compositions were prepared into a 2% (w / w) solution using DMEM culture medium containing 10% serum as solvent.

[0130] (3) Positive control: Prepare a 10% EGF solution using DMEM culture medium containing 10% serum as a solvent.

[0131] 3. Testing steps:

[0132] (1) Cell seeding and culture: The cell suspension with the adjusted concentration is seeded into the prepared culture plate (12-well plate). An appropriate volume (such as 2 mL or 1 mL) of cell suspension is added to each well to ensure that the cells are evenly distributed at the bottom of the well. The cells are cultured overnight in a 37°C, 5% CO2 incubator to allow the cells to adhere and grow to form a monolayer.

[0133] (2) Scratching operation: Take out the culture plate and, in a clean bench, use a sterile micropipette tip or a special scratching tool to gently scratch a straight line in the same direction in the central area of ​​the monolayer adherent cells. The width of the scratch should be relatively consistent (about 0.5-1 mm), and try to avoid scratching the bottom of the culture plate.

[0134] Wash the cells gently with PBS 2-3 times to remove cell debris, then replace with serum-free DMEM medium and continue culturing in an incubator for 0-2 hours (starvation treatment; the time can be adjusted according to cell type) to synchronize the cells to the quiescent phase and reduce the influence of serum on cell migration.

[0135] (3) After the starvation treatment, carefully aspirate the serum-free DMEM culture medium and add samples according to the experimental design groups:

[0136] Negative control group: Add DMEM culture medium containing 10% serum.

[0137] Sample group: solutions of 2% by mass of the platinum tea compositions of Examples 1-12 and Comparative Examples 1-4 were added respectively (using DMEM culture medium containing 10% serum as solvent).

[0138] Positive control group: 10% EGF solution was added (using DMEM culture medium containing 10% serum as the solvent).

[0139] When adding the sample, it should be added slowly drop by drop to avoid dispersing the cells or causing excessively high local concentrations.

[0140] (4) The culture plates were removed and photographed at 0 hours (immediately after sample addition) and 24 hours after culture. Three areas were randomly selected, and the mean area of ​​the intercellular scratches was calculated. The cell migration rate (wound healing rate) was calculated from the mean area of ​​the intercellular scratches at 0h and 18h. The cell migration rate (wound healing rate) expresses the repair effect of the sample. The higher the cell migration rate (wound healing rate), the better the repair effect.

[0141] The cell migration rate (wound healing rate) was calculated according to formula (3).

[0142] Cell migration rate (%) = (Initial scratch width or area - Scratch width or area at observation time) / Initial scratch width or area × 100%

[0143] —Equation (3)

[0144] Table 10

[0145]

[0146]

[0147] The test results are shown in Table 10. Compared with the blank control group, the platinum tea compositions prepared in the examples and comparative examples of this invention all have certain repair capabilities and can promote the migration of HaCaT human keratinocytes. Among them, the platinum tea compositions of Examples 1-12 have better repair effects than those of Comparative Examples 1-4, indicating that the combination of the fermentation product filtrate of *Tea japonica*, carob seed extract, and decarboxylated carnosine hydrochloride has a synergistic effect and can effectively enhance the repair effect of the final product. The lack of any one of the components results in poor repair effects of the obtained platinum tea compositions. The platinum tea composition of Example 10 has the best effect, that is, the optimal combination ratio is *Tea japonica* fermentation product filtrate: carob seed extract: decarboxylated carnosine hydrochloride = 15:5:2.

[0148] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A platinum tea composition, characterized in that, It consists of the following components in parts by weight: 7-15 parts of fermented product filtrate of golden flower white tea, 3-6 parts of carob seed extract, and 1.4-2 parts of decarboxylated carnosine hydrochloride.

2. The platinum tea composition as described in claim 1, characterized in that, It consists of the following components in parts by weight: 15 parts of fermented product filtrate of golden flower white tea, 5 parts of carob seed extract, and 2 parts of decarboxylated carnosine hydrochloride.

3. The use of the platinum tea composition according to any one of claims 1-2 in the preparation of cosmetics.

4. A cosmetic product, characterized in that, Includes the platinum tea composition as described in any one of claims 1-2.

5. The cosmetic product as described in claim 4, characterized in that, The amount of the platinum tea composition added to cosmetics is 7.00%-23.00%.

6. The cosmetic product as described in claim 5, characterized in that, The amount of the platinum tea composition added to the cosmetic is 22.00%.

7. The cosmetic product as described in claim 4, characterized in that, The dosage forms of the cosmetics include water, lotion, and cream.

8. The cosmetic product as described in claim 4, characterized in that, The cosmetics also include excipients acceptable in daily chemical products.

9. The cosmetic product as described in claim 8, characterized in that, The excipients include at least one of emollients, dispersants, humectants, preservatives, emulsifying stabilizers, thickeners, and pH adjusters.

Citation Information

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