Extraction process of peony seed oil

Abstract

The present application belongs to the technical field of plant oil extraction, and particularly relates to an extraction process of peony seed oil, and specifically comprises the following steps: S1, drying, shelling and grinding peony seeds; S2, adding water and an additive, and performing segmented microwave treatment; S3, adjusting pH and adding a composite enzyme preparation for ultrasonic enzymolysis; S4, centrifuging the enzymolysis, collecting upper oil and emulsion; freezing the emulsion, then performing secondary centrifugation after thawing, collecting upper oil, and combining the oils obtained after the two centrifugations to obtain the peony seed oil. The extraction process of peony seed oil provided by the present application adopts a low-temperature treatment process throughout, is safe, environmentally friendly and efficient, maximally reduces the loss of active substances in the preparation process, effectively improves the oil yield and quality indexes of peony seed oil, and the prepared peony seed oil has excellent antioxidant performance and can play a role in reducing inflammatory response, improving blood lipid condition and protecting the cardiovascular system.

Description

Technical Field

[0001] The invention belongs to the technical field of plant oil extraction, and particularly relates to an extraction process for peony seed oil. Background Art

[0002] Peony seed oil is a golden, transparent liquid extracted and refined from peony seeds. It is rich in unsaturated fatty acids, paeonol, phytosterols, squalene, and other beneficial ingredients. Unsaturated fatty acids account for over 85%, primarily α-linolenic acid. Consuming peony seed oil has the benefits of promoting blood circulation, removing blood stasis, nourishing yin, and reducing internal heat. It also has antioxidant properties, promotes cell regeneration, alleviates acute liver damage, regulates the immune system, and lowers blood lipids, effectively preventing diabetes. my country is rich in peony resources, and the extraction and bioactivity of peony seed oil have shown promise in preventing oxidative aging, diabetes, and hyperlipidemia.

[0003] Currently, peony seed oil extraction technologies primarily include physical pressing and organic solvent extraction. Traditional pressing processes have low oil yields and high impurity content, and the high temperatures during the extrusion process can destroy unsaturated fatty acids and other trace active substances. Conventional organic solvent extraction methods also suffer from residual solvents, and the high temperatures generated during the desolventizing process can damage the oil's nutritional value. Chinese patent application CN113481051A discloses a method for improving the quality of peony seed oil, comprising the following steps: Step 1: roasting peony seeds at a temperature of 110-170°C for 10-30 minutes; Step 2: adjusting the preheating temperature of the oil press to 150°C. After the preheating is complete, the peony seeds processed in Step 1 are pressed at 50°C to produce crude peony seed oil; Step 3: subjecting the crude peony seed oil to four steps: water degumming, alkali refining and deacidification, crude oil decolorization, and crude oil deodorization, to obtain refined peony seed oil. This method involves multiple complex refining steps, is difficult to perform, and may result in the loss of some active ingredients.

[0004] Chinese patent application CN109370763A discloses a method for preparing peony seed oil, comprising the following steps: 1) shelling and crushing peony seeds to obtain peony kernel powder; 2) mixing the peony kernel powder described in step 1) with an aqueous enzyme preparation solution and performing enzymatic hydrolysis to obtain an enzymatic hydrolyzate; 3) drying and pressing the enzymatic hydrolyzate described in step 2) to obtain peony seed oil; the enzyme preparation in step 2) includes one or more of cellulase, neutral protease, alkaline protease, α-amylase, pectinase, and hemicellulase; and the pressing temperature in step 3) is 50-80°C. However, this method uses only a single enzyme for enzymatic hydrolysis, resulting in inadequate hydrolysis of the peony seeds, which affects oil release and yield.

[0005] Therefore, it is necessary to provide a peony seed oil extraction process that is simple, easy to operate, and has mild conditions, which can increase the oil yield of peony seed oil and improve the quality of the prepared peony seed oil. Summary of the Invention

[0006] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a process for extracting peony seed oil.

[0007] The technical solution of the present invention is:

[0008] A process for extracting peony seed oil comprises the following steps:

[0009] S1, drying, shelling, and grinding peony seeds to obtain peony seed powder;

[0010] S2. Add the peony seed powder obtained in step S1 to water, then add an auxiliary agent, stir evenly, and perform segmented microwave treatment to obtain a pretreated peony seed powder mixture;

[0011] S3, adjusting the pH of the peony seed powder mixture obtained in step S2, then adding a complex enzyme preparation for ultrasonic enzymolysis, and then inactivating the enzyme to obtain an enzymatic solution;

[0012] S4, the enzymolyzed solution obtained in step S3 is cooled to room temperature, centrifuged, and the upper layer of fat and emulsion is collected; the emulsion is frozen for a period of time, then thawed and centrifuged twice to collect the upper layer of fat, and the fats obtained after the two centrifugations are mixed to obtain peony seed oil.

[0013] Furthermore, the auxiliary agent in step S2 is composed of 1-hexyl-3-methylimidazolium chloride, alkyl glycoside and mannan in a weight ratio of 1.4-2.0:0.30-0.75:0.7-1.0, preferably 1.7:0.5:0.8.

[0014] Furthermore, in step S1, the peony seeds are dried at a low temperature of 40-50° C. to a moisture content of 4-6%.

[0015] Furthermore, in step S1, the shelled peony seeds are ground and then passed through a 20-40 mesh sieve.

[0016] Furthermore, the amount of water added in step S2 is 4 to 6 times the weight of the peony seed powder, preferably 5 times.

[0017] Furthermore, the amount of the additive added in step S2 is 2-4% of the total weight of the peony seed powder and water, preferably 3%.

[0018] Furthermore, the process of the segmented microwave treatment in step S2 is specifically as follows:

[0019] After the peony seed powder and the mixed solution are evenly stirred, soak for 10-20 minutes, and then treat under a microwave power of 500-600 W for 1.5-2 minutes; continue soaking for 40-60 minutes, and then treat under a microwave power of 300-400 W for 3-5 minutes.

[0020] Furthermore, in step S3, the pH of the peony seed powder mixture is adjusted to 6.0-7.0.

[0021] Furthermore, the complex enzyme preparation in step S3 is a combination of neutral protease, cellulase and Candida antarctica lipase.

[0022] Furthermore, the addition amount of the neutral protease is 0.9~1.4%, preferably 1.2%, the addition amount of the cellulase is 0.6~0.9%, preferably 0.8%, and the addition amount of the Antarctic Candida lipase is 0.06~0.18%, preferably 0.13%, all based on the weight of the peony seed powder.

[0023] Furthermore, in step S3, the ultrasonic enzymatic hydrolysis temperature is 35-45° C., the time is 2-3 h, and the ultrasonic frequency is 20-40 kHz.

[0024] Furthermore, the enzyme inactivation process in step S3 is maintained at 90° C. for 15 min.

[0025] Furthermore, the rotation speed of the two centrifugations in step S4 is 10,000-12,000 rpm, and the centrifugation time is 15-25 min.

[0026] Furthermore, in step S4, the emulsion is frozen at -30 to -20°C for 20 to 24 hours, and then taken out and thawed at 50°C.

[0027] The present invention provides a peony seed oil extraction process. The core of the process is to use an aqueous enzymatic method to extract the oil. The overall reaction conditions are mild, efficient, and environmentally friendly. It can minimize the loss of active ingredients in the peony seed oil, especially helping to increase the content of unsaturated fatty acids (such as α-linolenic acid). At the same time, no organic solvents are used, reducing the risk of environmental pollution. After quality testing, the peony seed oil produced by the present invention was free of solvent residues, toxic substances such as lead, arsenic, benzopyrene, and aflatoxin B1, and pesticide residues such as chlordane, dimethoate, procymidone, haloxyfop-ethyl, high-efficiency haloxyfop-ethyl, fenthion (fenthion, fenthion sulfone, fenthion sulfoxide), and diquat.

[0028] In the peony seed oil extraction process of the present invention, solution immersion and ultrasonic enzymatic hydrolysis are two key steps. First, by rationally selecting the auxiliary agents in the immersion solution (composed of 1-hexyl-3-methylimidazolium chloride, alkyl glycoside, and mannan in a weight ratio of 1.4-2.0:0.30-0.75:0.7-1.0), and performing segmented microwave treatment, the oil yield and α-linolenic acid content of the peony seeds can be significantly improved, avoiding long-term high-temperature treatment, effectively reducing the loss of active ingredients, and simultaneously ensuring the effectiveness of the enzymatic hydrolysis process and improving the efficiency of subsequent ultrasonic enzymatic hydrolysis.

[0029] The added 1-hexyl-3-methylimidazolium chloride is an ionic liquid with excellent solubility and permeability, which can effectively destroy plant cell walls. The added alkyl glycoside is a green surfactant that can adsorb at the water-oil interface to form a molecular film, reducing the tension at the water-oil interface. Because plant cell walls contain a large amount of lipid components, alkyl glycoside can further help the aqueous phase penetrate the cell more easily by reducing the interfacial tension, destroying the cell wall structure and promoting the release of oil. The three can also produce a synergistic effect. During the subsequent enzymatic hydrolysis process, by reducing the interfacial tension between the enzyme and the substrate, the contact between the enzyme and the substrate is enhanced, thereby improving the efficiency of the enzymatic hydrolysis.

[0030] At the same time, during the research process, the present invention also found that by adding the auxiliary agent provided by the present invention to soak the peony seeds and combining them with microwave treatment, the oil and water phase can be well separated during the subsequent processing process, the occurrence of excessive emulsification can be avoided, and the obtained emulsion is easier to demulsify, thereby reducing the oil loss caused by emulsification, reducing the difficulty of separation, and ensuring a high extraction rate and purity of the oil; and it can be seen from experimental data that this method can also effectively reduce the generation of free fatty acids and prevent the oxidative degradation of oils, especially the protective effect on unsaturated fatty acids (such as α-linolenic acid) is more obvious, ensuring the high quality and stability of the oil.

[0031] Furthermore, in the present invention, a small amount of Antarctic Candida lipase is added during the ultrasonic enzymatic hydrolysis process while adding neutral protease and cellulase. By optimizing conditions such as the enzyme ratio, enzymatic hydrolysis temperature, pH value, enzymatic hydrolysis time and ultrasonic frequency, the cell wall structure in the peony seed kernel powder is completely destroyed, the release of oil is promoted, and the yield of oil and the content of unsaturated fatty acids such as α-linolenic acid are significantly improved. By adding the low-activity lipase Antarctic Candida lipase, the release of oil can be further promoted, and at the same time, excessive hydrolysis of triglycerides can be avoided, thereby reducing the generation of free fatty acids, reducing the acid value and peroxide value of the prepared peony seed oil, etc., ensuring the quality of the peony seed oil and improving its antioxidant properties.

[0032] Compared with the prior art, the extraction process of peony seed oil provided by the present invention has the following advantages:

[0033] (1) The extraction process of peony seed oil provided by the present invention uses two core steps of microwave immersion and ultrasonic enzymatic hydrolysis to treat peony seed powder, adopts a low-temperature treatment process throughout the process, and does not use organic solvents. It is safe, environmentally friendly, and efficient, and minimizes the loss of active substances in the preparation process, effectively improving the oil yield and quality indicators of peony seed oil.

[0034] (2) The peony seed oil prepared by the method of the present invention has excellent antioxidant properties and can play a positive and effective role in reducing inflammatory reactions, improving blood lipid status, and protecting the cardiovascular and cerebrovascular systems. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 DPPH free radical scavenging rate (%) of the peony seed oil prepared in the examples of the present invention and the comparative example;

[0036] Figure 2 The superoxide anion radical (·O2 - ) clearance rate (%). DETAILED DESCRIPTION

[0037] The present invention is further illustrated below through the description of specific implementation methods, but this is not a limitation of the present invention. Those skilled in the art can make various modifications or improvements based on the basic idea of ​​the present invention, but as long as they do not deviate from the basic idea of ​​the present invention, they are all within the scope of protection of the present invention.

[0038] In the following examples and comparative examples, reagents not otherwise specified are conventional reagents and can be purchased from conventional reagent production and sales companies. The methods used, unless otherwise specified, are all based on existing technologies. Information on some raw material manufacturers is as follows:

[0039] The peony used in the embodiment of the present invention is the Fengdan peony from Heze, Shandong;

[0040] 1-Hexyl-3-methylimidazolium chloride, CAS No. 171058-17-6; 1-octyl-3-methylimidazolium bromide, CAS No. 61545-99-1, were purchased from Wuhan Xinxin Jiali Biotechnology Co., Ltd.

[0041] Alkyl glycoside, CAS number 161074-87-9, Shandong Haizhou Bioengineering Co., Ltd.;

[0042] Mannan, CAS No. 9036-88-8, Shandong Xiya Chemical Co., Ltd.;

[0043] Neutral protease, CAS No. 9068-59-1; cellulase, CAS No. 9012-54-8, were purchased from Weifang Ruichen Biotechnology Co., Ltd.

[0044] Candida antarctica lipase has a CAS number of 9001-62-1 and is from Rebo (Shanghai) Biochemical Technology Co., Ltd.

[0045] Example 1

[0046] A process for extracting peony seed oil comprises the following steps:

[0047] S1, low-temperature drying the peony seeds at 40 ° C to a moisture content of 4%, shelling, grinding through a 20-mesh sieve to obtain peony seed powder;

[0048] S2, adding the peony seed powder obtained in step S1 to 4 times its weight in water, then adding an auxiliary agent (the amount of the auxiliary agent added is 2% of the total weight of the peony seed powder and water, and the auxiliary agent consists of 1-hexyl-3-methylimidazolium chloride, alkyl glycoside and mannan in a weight ratio of 1.4:0.30:1.0), stirring evenly, and performing segmented microwave treatment: first soaking for 10 min, then treating at a microwave power of 500 W for 2 min; continuing to soak for 40 min, and then treating at a microwave power of 300 W for 5 min to obtain a pretreated peony seed powder mixture;

[0049] S3, adjusting the pH of the peony seed powder mixture obtained in step S2 to 6.0-7.0, then adding 0.9% neutral protease, 0.6% cellulase and 0.06% Antarctic Candida lipase, all based on the weight of the peony seeds; stirring evenly, and performing ultrasonic enzymolysis at a temperature of 35° C. and an ultrasonic frequency of 20 kHz for 3 h; then maintaining the treatment at 90° C. for 15 min to obtain an enzymatic solution;

[0050] S4, the enzymatic hydrolyzate obtained in step S3 was cooled to room temperature, and the mixture was centrifuged at 10,000 rpm for 25 min to collect the upper grease and emulsion; the emulsion was frozen at -20 ° C for 24 h, thawed at 50 ° C after taking it out, and then centrifuged at 10,000 rpm for 15 min to collect the upper grease, and the grease obtained after the two centrifugations was mixed to obtain peony seed oil.

[0051] Example 2

[0052] A process for extracting peony seed oil comprises the following steps:

[0053] S1, low-temperature drying the peony seeds at 45 ° C to a moisture content of 5%, shelling, grinding through a 30-mesh sieve to obtain peony seed powder;

[0054] S2, adding the peony seed powder obtained in step S1 to 5 times its weight in water, then adding an auxiliary agent (the amount of the auxiliary agent added is 3% of the total weight of the peony seed powder and water, and the auxiliary agent consists of 1-hexyl-3-methylimidazolium chloride, alkyl glycoside and mannan in a weight ratio of 1.7:0.5:0.8), stirring evenly, and performing segmented microwave treatment: first soaking for 15 min, then treating at a microwave power of 550 W for 1.8 min; continuing to soak for 50 min, and then treating at a microwave power of 350 W for 4 min to obtain a pretreated peony seed powder mixture;

[0055] S3, adjusting the pH of the peony seed powder mixture obtained in step S2 to 6.0-7.0, then adding 1.2% neutral protease, 0.8% cellulase and 0.13% Antarctic Candida lipase, all based on the weight of the peony seeds; stirring evenly, and performing ultrasonic enzymolysis at a temperature of 40° C. and an ultrasonic frequency of 30 kHz for 2.5 h; then maintaining the treatment at 90° C. for 15 min to obtain an enzymatic solution;

[0056] S4, the enzymatic hydrolyzate obtained in step S3 was cooled to room temperature, and the mixture was centrifuged at a speed of 11000 rpm for 20 min to collect the upper grease and emulsion; the emulsion was frozen at -25 ° C for 24 h, taken out and thawed at 50 ° C, and then centrifuged at a speed of 12000 rpm for 20 min to collect the upper grease, and the greases obtained after the two centrifugations were mixed to obtain peony seed oil.

[0057] Example 3

[0058] A process for extracting peony seed oil comprises the following steps:

[0059] S1, low-temperature drying the peony seeds at 50 ° C to a moisture content of 6%, shelling, and grinding through a 40-mesh sieve to obtain peony seed powder;

[0060] S2, adding the peony seed powder obtained in step S1 to 6 times its weight in water, then adding an auxiliary agent (the amount of the auxiliary agent added is 4% of the total weight of the peony seed powder and water, and the auxiliary agent consists of 1-hexyl-3-methylimidazolium chloride, alkyl glycoside and mannan in a weight ratio of 2.0:0.75:0.7), stirring evenly, and performing segmented microwave treatment: first soaking for 20 min, then treating at a microwave power of 600 W for 1.5 min; continuing to soak for 60 min, and then treating at a microwave power of 400 W for 3 min to obtain a pretreated peony seed powder mixture;

[0061] S3, the pH of the obtained peony seed powder mixed solution in step S2 is adjusted to 6.0-7.0, then 1.4% of neutral protease, 0.9% of cellulase and 0.18% of Candida antarctica lipase are added, all based on the weight of peony seeds; after uniform stirring, ultrasonic enzymolysis is carried out under the condition of 45°C and ultrasonic frequency of 40 kHz for 2 h; then it is treated at 90°C for 15 min, and an enzymolysis solution is obtained;

[0062] S4, the enzymolysis solution obtained in step S3 is cooled to room temperature, and the upper layer of oil and emulsion is collected by centrifugation at a speed of 12000 rpm for 15 min; the emulsion is frozen at-30°C for 20 h, then thawed at 50°C, and then centrifuged at a speed of 12000 rpm for 20 min, and the upper layer of oil is collected, and the oils obtained by the two times of centrifugation are mixed, and peony seed oil is obtained.

[0063] Comparative Example 1

[0064] Compared with Example 2, the difference is that the 1-hexyl-3-methyl imidazole chloride salt in the additive added in step S2 is replaced by 1-octyl-3-methyl imidazole bromide salt, that is, the additive is composed of 1-octyl-3-methyl imidazole bromide salt, alkyl glycoside and mannose in a weight ratio of 1.7:0.5:0.8, and other components and preparation methods are the same as those of Example 2.

[0065] Comparative Example 2

[0066] Compared with Example 2, the difference is that the alkyl glycoside is not added in the additive of step S2, but the amount of mannose is correspondingly increased, that is, the additive is composed of 1-hexyl-3-methyl imidazole chloride salt and mannose in a weight ratio of 1.7:1.3, and other components and preparation methods are the same as those of Example 2.

[0067] Comparative Example 3

[0068] Compared with Example 2, the difference is that the Candida antarctica lipase is not added in the compound enzyme preparation in step S3, but the amount of cellulase is correspondingly increased, that is, the compound enzyme preparation is composed of neutral protease and cellulase, the addition amount of the neutral protease is 1.2% of the weight of peony seed powder, and the addition amount of the cellulase is 0.93% of the weight of peony seed powder, and other components and preparation methods are the same as those of Example 2.

[0069] Comparative Example 4

[0070] Compared with Example 2, the difference is that the microwave treatment process in step S2 is not segmented, that is, in step S2, the peony seed powder is added into water, then the additive is added and stirred uniformly, then soaked for 60 min, and then treated under the microwave power of 500 W for 8 min. Other components and preparation methods are the same as those of Example 2.

[0071] Test Example 1: Quality testing of peony seed oil prepared in the present invention

[0072] 1. Test Materials: Peony seed oils prepared in Examples 1 to 3 and Comparative Examples 1 to 4 of the present invention.

[0073] 2. Test Method: The oil yield, basic composition, main physical parameters, and quality indicators of the peony seed oils prepared in Examples 1 to 3 and Comparative Examples 1 to 4 of the present invention were tested. The oil yield of the peony seed oil was calculated using the following formula:

[0074] Oil yield = peony seed oil mass / peony seed mass × 100%. The test results are shown in Table 1.

[0075] The basic composition, main physical parameters, and quality indicators of peony seed oil were tested according to the methods specified in the national standard GB / T 40622-2021 "Peony Seed Oil". The basic composition, main physical parameters, and test results of peony seed oil are shown in Table 2, the quality index regulations are shown in Table 3, and the quality index test results are shown in Table 4. Table 1

[0076] Group Oil out rate (%) Example 1 25.1 Example 2 26.3 Example 3 25.8 Comparative Example 1 19.6 Comparative Example 2 20.8 Comparative Example 3 21.5 Comparative Example 4 23.4

[0077] Table 2

[0078]

[0079] Table 3

[0080] Table 4

[0081] As shown in Tables 1 to 4, the oil yield of the peony seed oil obtained by the method of Examples 1 to 3 of the present invention is more than 25%, and the basic composition and main physical parameter indices and quality indicators are in line with the provisions of the national standard GB / T 40622-2021 "Peony Seed Oil". The obtained peony seed oil is clear and transparent light yellow, has the inherent smell and taste of peony seed oil, has no odor, low acid value and peroxide value, and has a high content of unsaturated fatty acids, especially the content of α-linolenic acid is greater than 42%, indicating that the quality of peony seed oil can be effectively improved by the process provided by the present invention, while the oil yield is increased, the active ingredients in the peony seed oil are retained to the greatest extent. When the extraction method is changed in Comparative Examples 1 to 4, the oil yield and quality indicators of the obtained peony seed oil all decrease to varying degrees.

[0082] Test Example 2: Antioxidant Performance Test of Peony Seed Oil Prepared by the Present Invention

[0083] 1. Test Materials: Peony seed oils prepared in Examples 1 to 3 and Comparative Examples 1 to 4 of the present invention.

[0084] 2. Test method:

[0085] (1) DPPH free radical scavenging rate:

[0086] The peony seed oils prepared in Examples 1 to 3 and Comparative Examples 1 to 4 of the present invention were diluted with anhydrous ethanol to a concentration of 10% and used as test samples. The specific reaction system is as follows:

[0087] 2 mL of the above-prepared sample + 2 mL of DPPH, recorded as A1;

[0088] 2 mL of anhydrous ethanol + 2 mL of DPPH, recorded as A2;

[0089] 2 mL sample + 2 mL anhydrous ethanol, recorded as A0;

[0090] Each reaction solution was mixed evenly and incubated at 25°C in the dark for 30 min. The absorbance was measured at 517 nm. The formula for DPPH free radical scavenging rate is as follows:

[0091] DPPH free radical scavenging rate (%) = [1-(A1-A0) / A2] × 100%

[0092] The test results of the DPPH free radical scavenging rate are shown in Table 5 and Figure 1 As shown in Table 5

[0093] Group DPPH radical scavenging rate (%) Example 1 94.5 Example 2 95.2 Example 3 94.6 Comparative Example 1 85.6 Comparative Example 2 90.5 Comparative Example 3 86.1 Comparative Example 4 91.3

[0094] (2) Superoxide anion free radical (·O2 - ) Clearance rate:

[0095] The peony seed oils prepared in Examples 1 to 3 and Comparative Examples 1 to 4 of the present invention were diluted with anhydrous ethanol to a concentration of 10% to prepare solutions as test samples. 1 mL of each test sample was added to 4.5 mL of Tris-HCl solution (pH = 8.2, 0.05 mol / L), and allowed to stand at room temperature for 10 minutes. 0.1 mL of 6 mmol / L pyrogallol solution was then added to each solution, mixed thoroughly, and reacted for 3 minutes. Finally, 0.1 mL of 1 mol / L hydrochloric acid solution was added to each solution. The absorbance A was measured at a wavelength of 325 nm. m ; Use distilled water instead of the sample to measure the absorbance A n ; Use distilled water instead of pyrogallol to measure the reference absorbance A q ·O2 - The calculation formula of clearance rate is as follows: O2 - Clearance rate (%) = {[1-(Am -A n )] / A q}×100%

[0096] Said O2 - The test results of clearance rate are shown in Table 6 and Figure 2 As shown in Table 6

[0097]

[0098] As shown in Table 5 and Table 6, the peony seed oils prepared in Examples 1 to 3 of the present invention have an inhibitory effect on DPPH free radicals and superoxide anion free radicals (·O2 - ) were above 94% and 85% respectively, indicating that the peony seed oil prepared by the extraction process of the present invention has excellent antioxidant properties and can play a positive and effective role in reducing inflammatory reactions, improving blood lipid status and protecting the cardiovascular and cerebrovascular systems.

[0099] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A process for extracting peony seed oil, characterized in that: The method comprises the following preparation steps: S1, drying, shelling, and grinding peony seeds to obtain peony seed powder; S2. Add the peony seed powder obtained in step S1 to water, then add an auxiliary agent, stir evenly, and perform segmented microwave treatment to obtain a pretreated peony seed powder mixture; S3, adjusting the pH of the peony seed powder mixture obtained in step S2, then adding a complex enzyme preparation for ultrasonic enzymolysis, and then inactivating the enzyme to obtain an enzymatic solution; S4, the enzymatic hydrolyzate obtained in step S3 is cooled to room temperature, centrifuged, and the upper layer of fat and emulsion are collected; the emulsion is frozen for a period of time, then centrifuged twice after thawing, the upper layer of fat is collected, and the fats obtained after the two centrifugations are mixed to obtain peony seed oil; The auxiliary agent in step S2 is composed of 1-hexyl-3-methylimidazolium chloride, alkyl glycoside and mannan in a weight ratio of 1.4-2.0:0.30-0.75:0.7-1.0; The amount of water added in step S2 is 4 to 6 times the weight of the peony seed powder; the amount of the auxiliary agent added in step S2 is 2 to 4% of the total weight of the peony seed powder and water; The process of the segmented microwave treatment in step S2 is specifically as follows: after the peony seed powder and the mixed solution are uniformly stirred, soaking for 10 to 20 minutes, and then treating at a microwave power of 500 to 600 W for 1.5 to 2 minutes; continuing to soak for 40 to 60 minutes, and then treating at a microwave power of 300 to 400 W for 3 to 5 minutes; In step S3, the pH of the peony seed powder mixture is adjusted to 6.0-7.0; the complex enzyme preparation in step S3 is a combination of neutral protease, cellulase and Antarctic Candida lipase; The amount of the neutral protease added in step S3 is 0.9-1.4%, the amount of the cellulase added is 0.6-0.9%, and the amount of the Antarctic Candida lipase added is 0.06-0.18%, all based on the weight of the peony seed powder.

2. The extraction process of peony seed oil according to claim 1, wherein In the step S1, the shelled peony seeds are ground and then passed through a 20-40 mesh sieve.

3. The extraction process of peony seed oil according to claim 1, wherein In step S3, the ultrasonic enzymatic hydrolysis temperature is 35-45° C., the time is 2-3 h, and the ultrasonic frequency is 20-40 kHz.

4. The extraction process of peony seed oil according to claim 1, wherein The rotation speed of the two centrifugations in step S4 is 10,000-12,000 rpm, and the centrifugation time is 15-25 min.

5. The extraction process of peony seed oil according to claim 1, wherein In step S4, the emulsion is frozen at -30 to -20°C for 20 to 24 hours, and then taken out and thawed at 50°C.

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