Screening and preparation method of anti-aging product formula

Abstract

The invention relates to the technical field of anti-aging products, in particular to screening of an anti-aging product formula, which comprises the following steps: step 1, carrying out a single-component test; 2, compounding according to a single-component test result to determine a compounding formula of the product; step 3, performing efficacy testing on the compound formula; according to the invention, after a single component test is carried out on a plurality of extracts, a single component and a compound composition of the formula are screened through a cell model, a compound formula with a remarkable effect is formed through optimized combination, the compound formula is subjected to an efficacy test, the compound formula of the product is finally determined, and scientific data is taken as a basis, so that the compound formula of the product is obtained. The anti-aging product capable of effectively resisting free radicals and protecting mitochondria is formed, the anti-aging effect of the components is maximized, and personalized anti-aging products capable of meeting diversified requirements can be developed according to individual differences, living habits and different environmental factors.

Description

Technical Field

[0001] The present invention relates to the technical field of anti-aging products, and in particular to a method for screening and preparing an anti-aging product formula. Background Art

[0002] With the development of society and the improvement of people's living standards, anti-aging has become one of the hot issues of modern people. Aging is a complex biological process involving multiple cell damages, metabolic disorders and changes in gene expression. These changes can lead to significant appearance characteristics such as sagging skin, increased wrinkles, pigmentation, and a gradual decline in body functions. Therefore, delaying the aging process and maintaining youthful vitality has become an urgent need for people.

[0003] There are many kinds of existing anti-aging products, including oral health care products, topical skin care products and injectable drugs. However, most anti-aging products on the market mainly rely on a single or a small amount of active ingredients, such as vitamin C, hyaluronic acid, collagen, etc. Although these ingredients can improve the skin condition to a certain extent, their anti-aging effects are often limited. In addition, these products usually have problems such as low absorption efficiency, poor bioavailability, and easy to cause allergic reactions, resulting in unsatisfactory anti-aging effects. With the advancement of biotechnology and chemical synthesis technology, more and more new anti-aging active ingredients have been discovered and applied to the research and development of anti-aging products, such as polypeptide compounds, plant extracts, nanomaterials and their complexes. These ingredients have shown significant potential in anti-oxidation, anti-inflammation, and promoting cell repair and regeneration. However, how to screen out the best combination of active ingredients and maximize the anti-aging effects of these ingredients through reasonable formula design and preparation process is still a technical problem that needs to be solved urgently. In addition, due to individual differences, living habits and environmental factors, the needs of different populations for anti-aging products are also significantly different, and the prepared anti-aging products cannot meet the diverse and personalized anti-aging needs.

[0004] Therefore, in order to address the above-mentioned problem that the best combination of active ingredients cannot be screened out and the maximization of the anti-aging effect cannot be guaranteed, a method for screening and preparing the anti-aging product formula can be designed. Summary of the invention

[0005] In order to overcome the problem of being unable to screen out the best combination of active ingredients and ensuring the maximum anti-aging effect.

[0006] The technical solution of the present invention is: a screening of anti-aging product formula, the steps of which are as follows:

[0007] Step 1: Perform single component tests on hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, bilberry fruit extract, prickly pear cactus stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, scutellaria root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract, and Tremella fuciformis extract;

[0008] Step 2: According to the single component test results, compound and determine the compound formula of the product;

[0009] Step 3: Compound the formula for efficacy testing:

[0010] Human dermal fibroblasts (NHDF) were seeded into glass-bottomed confocal dishes, and cultured overnight under specified conditions using Dulbecco's Modified Eagle Medium (DMEM) plus 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin (PS). The cell culture medium was removed the next day, and equal amounts of PBS and compound formula were prepared as the control group and compound formula group. Equal amounts of PBS and formula were mixed with equal amounts of basal culture medium DMEM and added to the confocal dish, and cultured in an incubator for 4 hours. After the time was up, the cell culture medium was removed, and the cells were washed twice with PBS. The cells were stained using a mitochondrial membrane potential detection kit (JC-1), and then the cells were imaged using a confocal laser microscope (Leica, Germany);

[0011] During cell aging or apoptosis, the decline of mitochondrial membrane potential is usually one of the early events. JC-1 is a mitochondrial membrane potential fluorescent probe: under high mitochondrial membrane potential, JC-1 aggregates to form J-aggregates, emitting red fluorescence (Ex / Em=585 / 590nm); under low membrane potential, JC-1 exists in monomeric form, emitting green fluorescence (Ex / Em=510 / 527nm). Therefore, the red / green fluorescence ratio of JC-1 is used to quantitatively analyze the changes in mitochondrial membrane potential. Combined with the imaging results, if the cells show a significant increase in the red fluorescence signal compared with the control group, it means that the group of cells has achieved effective anti-free radical and mitochondrial protection after treatment with the compound formula; if not, it means that the compound formula has no obvious effect on the cells to achieve effective anti-free radical and mitochondrial protection.

[0012] Preferably, the conditions for overnight culture are: 37°C, 5% CO2.

[0013] The method for preparing an anti-aging product comprises screening the anti-aging product formula as described above, and the steps are as follows:

[0014] Step 1: Prepare the raw materials of phase A, phase B and phase C according to the compound formula

[0015] Phase A: water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glyceryl polyether-26;

[0016] Phase B: Hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, vaccinium fruit extract, opuntia cochinchinensis stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, scutellaria baicalensis root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract and Tremella fuciformis extract;

[0017] Phase C: niacinamide, bosaicin, peptides, bifida yeast and 1,2-hexanediol;

[0018] Step 2: Add water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glycerol polyether-26 into a beaker, heat to 80-85°C, stir until dissolved, then continue stirring for 30 minutes, cool to 45°C, and obtain a mixed solution A;

[0019] Step 3: Add phase B into water to dissolve, then add to the mixed solution of step 2, add nicotinamide, bosaicin, peptide, bifida yeast and 1,2-hexanediol, continue stirring for 5 minutes to obtain a mixed solution B;

[0020] Step 4: After the mixed solution B is left to stand for 1 hour, prepare the funnel, filter paper, glass rod and receiving container, fold the filter paper twice into a fan shape, unfold it into a cone shape, put it into the funnel, moisten the filter paper with a small amount of distilled water, ensure that the filter paper and the funnel fit tightly, carefully pour the upper clear liquid slowly into the funnel along the glass rod against the side with more filter paper layers, not exceeding two-thirds of the filter paper, wash the precipitate with a small amount of washing liquid and stir and settle it thoroughly, repeat more than three times, transfer it to the filter paper each time, and finally wash the container containing the precipitate three times, transfer it to the funnel each time, take the supernatant, and then add the adsorption clarifier to the supernatant, and finally filter it through a microporous filter membrane to obtain an anti-aging product;

[0021] Step 5: Sterilize the anti-aging products and pack them into packaging containers according to the specified capacity.

[0022] Preferably, the anti-aging product is anti-aging essence water.

[0023] The beneficial effects of the present invention are as follows: after single-component testing of a variety of extracts, single components and compound compositions of the formula are screened through a cell model, and a compound formula with significant effects is formed through optimized combination, and the efficacy of the compound formula is tested to finally determine the compound formula of the product. Based on scientific data, an anti-aging product with effective resistance to free radicals and mitochondrial protection is formed, and the anti-aging effects of these ingredients are maximized. According to individual differences, living habits and environmental factors, personalized anti-aging products that can meet diverse needs can be developed. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Shown are images of cells in the control group and the compound formula group of the present invention. DETAILED DESCRIPTION

[0025] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] See also Figure 1 The present invention provides an embodiment: a method for screening an anti-aging product formula, the steps of which are as follows:

[0027] Step 1: Perform single component tests on hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, bilberry fruit extract, prickly pear cactus stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, scutellaria root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract, and Tremella fuciformis extract;

[0028] Step 2: According to the single component test results, compound and determine the compound formula of the product;

[0029] Step 3: Compound the formula for efficacy testing:

[0030] Human dermal fibroblasts (NHDF) were seeded into glass-bottomed confocal dishes, and cultured overnight at 37°C and 5% CO2 using DMEM (Dulbecco's Modified Eagle Medium) plus 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin (PS). The cell culture medium was removed the next day, and equal amounts of PBS and compound formula were prepared as the control group and compound formula group. Equal amounts of PBS and formula were mixed with equal amounts of basal culture medium DMEM and added to the confocal dish, and cultured in an incubator for 4 hours. After the time was up, the cell culture medium was removed, and the cells were washed twice with PBS. The cells were stained using a mitochondrial membrane potential detection kit (JC-1), and then the cells were imaged using a confocal laser microscope (Leica, Germany), as shown in Figure 2. Figure 1As shown, the cells treated with the compound formula showed a significant increase in red fluorescence signal compared with the control group, indicating that this group of cells achieved effective anti-free radical and mitochondrial protection after treatment.

[0031] Example 1

[0032] The method for preparing an anti-aging product comprises screening the anti-aging product formula as described above, and the steps are as follows:

[0033] Step 1: Prepare the following weight portions of raw materials for phase A, phase B and phase C according to the compound formula:

[0034] Phase A: water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glyceryl polyether-26;

[0035] Phase B: Hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, European bilberry fruit extract, twisted thorn cactus stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, scutellaria root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol;

[0036] Phase C: niacinamide, bosaicin, peptides, bifida yeast and 1,2-hexanediol;

[0037] Step 2: Add water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glycerol polyether-26 into a beaker, heat to 80°C and stir until dissolved, then continue stirring for 30 minutes, cool to 45°C, and obtain a mixed solution A;

[0038] Step 3: Add phase B into water to dissolve, then add to the mixed solution of step 2, add nicotinamide, bosaicin, peptide, bifida yeast and 1,2-hexanediol, continue stirring for 5 minutes to obtain a mixed solution B;

[0039] Step 4: After the mixed solution B is left to stand for 1 hour, prepare the funnel, filter paper, glass rod and receiving container, fold the filter paper twice into a fan shape, unfold it into a cone shape, put it into the funnel, moisten the filter paper with a small amount of distilled water, ensure that the filter paper and the funnel fit tightly, carefully pour the upper clear liquid slowly into the funnel along the glass rod against the side with more filter paper layers, not exceeding two-thirds of the filter paper, wash the precipitate with a small amount of washing liquid and stir and settle it thoroughly, repeat more than three times, transfer it to the filter paper each time, and finally wash the container containing the precipitate three times, transfer it to the funnel each time, take the supernatant, and then add the adsorption clarifier to the supernatant, and finally filter it through a microporous filter membrane to obtain an anti-aging product;

[0040] Step 5: Sterilize the anti-aging products and pack them into packaging containers according to the specified capacity.

[0041] Example 2

[0042] The method for preparing an anti-aging product comprises screening the anti-aging product formula as described above, and the steps are as follows:

[0043] Step 1: Prepare the raw materials of phase A, phase B and phase C according to the compound formula

[0044] Phase A: water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glyceryl polyether-26;

[0045] Phase B: Hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, vaccinium fruit extract, golden chamomile extract, purslane extract, scutellaria root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract and Tremella fuciformis extract;

[0046] Phase C: niacinamide, bosaicin, peptides, bifida yeast and 1,2-hexanediol;

[0047] Step 2: Add water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glycerol polyether-26 into a beaker, heat to 85°C and stir until dissolved, then continue stirring for 30 minutes, cool to 45°C, and obtain a mixed solution A;

[0048] Step 3: Add phase B into water to dissolve, then add to the mixed solution of step 2, add nicotinamide, bosaicin, peptide, bifida yeast and 1,2-hexanediol, continue stirring for 5 minutes to obtain a mixed solution B;

[0049] Step 4: After the mixed solution B is left to stand for 1 hour, prepare the funnel, filter paper, glass rod and receiving container, fold the filter paper twice into a fan shape, unfold it into a cone shape, put it into the funnel, moisten the filter paper with a small amount of distilled water, ensure that the filter paper and the funnel fit tightly, carefully pour the upper clear liquid slowly into the funnel along the glass rod against the side with more filter paper layers, not exceeding two-thirds of the filter paper, wash the precipitate with a small amount of washing liquid and stir and settle it thoroughly, repeat more than three times, transfer it to the filter paper each time, and finally wash the container containing the precipitate three times, transfer it to the funnel each time, take the supernatant, and then add the adsorption clarifier to the supernatant, and finally filter it through a microporous filter membrane to obtain an anti-aging product;

[0050] Step 5: Sterilize the anti-aging products and pack them into packaging containers according to the specified capacity.

[0051] Example 3

[0052] The method for preparing an anti-aging product comprises screening the anti-aging product formula as described above, and the steps are as follows:

[0053] Step 1: Prepare the raw materials of phase A, phase B and phase C according to the compound formula

[0054] Phase A: water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glyceryl polyether-26;

[0055] Phase B: Hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, vaccinium fruit extract, opuntia cactus stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract and Tremella fuciformis extract;

[0056] Phase C: niacinamide, bosaicin, peptides, bifida yeast and 1,2-hexanediol;

[0057] Step 2: Add water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glycerol polyether-26 into a beaker, heat to 82°C and stir until dissolved, then continue stirring for 30 minutes, cool to 45°C, and obtain a mixed solution A;

[0058] Step 3: Add phase B into water to dissolve, then add to the mixed solution of step 2, add nicotinamide, bosaicin, peptide, bifida yeast and 1,2-hexanediol, continue stirring for 5 minutes to obtain a mixed solution B;

[0059] Step 4: After the mixed solution B is left to stand for 1 hour, prepare the funnel, filter paper, glass rod and receiving container. Fold the filter paper twice into a fan shape, unfold it into a cone shape, and put it into the funnel. Wet the filter paper with a small amount of distilled water to ensure that the filter paper and the funnel fit tightly. Carefully pour the upper clear liquid along the glass rod against the side with more filter paper layers into the funnel, not exceeding two-thirds of the filter paper. Wash the precipitate with a small amount of washing solution and stir and settle it thoroughly.

[0060] Repeat three times or more, transfer to filter paper each time, and finally wash the container containing the precipitate three times, transfer to the funnel each time, take the supernatant, then add an adsorbent clarifier to the supernatant, and finally filter through a microporous filter membrane to obtain an anti-aging product;

[0061] Step 5: Sterilize the anti-aging products and pack them into packaging containers according to the specified capacity.

[0062] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the spirit of the present invention.

Claims

1. A method for screening an anti-aging product formula, characterized in that: The steps are as follows: Step 1: Perform single component tests on hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, bilberry fruit extract, prickly pear cactus stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, scutellaria root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract, and Tremella fuciformis extract; Step 2: According to the single component test results, compound and determine the compound formula of the product; Step 3: Compound the formula for efficacy testing: Human skin fibroblasts (NHDF) were seeded into glass-bottomed confocal dishes, and cultured overnight under specified conditions using Dulbecco's Modified Eagle Medium (DMEM) plus 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin (PS). The cell culture medium was removed the next day, and equal amounts of PBS and compound formula were prepared as the control group and compound formula group. Equal amounts of PBS and formula were mixed with equal amounts of basal culture medium DMEM and added to the confocal dish, and cultured in an incubator for 4 hours. After the time was up, the cell culture medium was removed, and the cells were washed twice with PBS. The cells were stained using a mitochondrial membrane potential detection kit (JC-1), and then the cells were imaged using a confocal laser microscope (Leica, Germany); During cell aging or apoptosis, the decline of mitochondrial membrane potential is usually one of the early events. JC-1 is a mitochondrial membrane potential fluorescent probe: under high mitochondrial membrane potential, JC-1 aggregates to form J-aggregates, emitting red fluorescence (Ex / Em=585 / 590nm); under low membrane potential, JC-1 exists in monomeric form, emitting green fluorescence (Ex / Em=510 / 527nm). Therefore, the red / green fluorescence ratio of JC-1 is used to quantitatively analyze the changes in mitochondrial membrane potential. Combined with the imaging results, if the cells show a significant increase in red fluorescence signals compared with the control group, it means that the group of cells has achieved effective anti-free radical and mitochondrial protection after being treated with the compound formula. If not, it means that the compound formula has no obvious effect on cells to achieve effective anti-free radical and mitochondrial protection.

2. The method for screening the anti-aging product formula according to claim 1, characterized in that: The designated conditions for overnight culture were: 37°C, 5% CO2.

3. A method for preparing an anti-aging product, characterized in that The method comprises screening the anti-aging product formula according to claim 1, wherein the steps are as follows: Step 1: Prepare the raw materials of phase A, phase B and phase C according to the compound formula Phase A: water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glyceryl polyether-26; Phase B: Hydroxypropyl tetrahydropyrantriol, tetrahydromethylpyrimidine carboxylic acid, glyceryl glucoside, rosemary leaf extract, vaccinium fruit extract, opuntia cochinchinensis stem extract, houttuynia cordata extract, golden chamomile extract, purslane extract, scutellaria baicalensis root extract, peony root bark extract, acetyl hexapeptide-8, xylitol, deoxyxylitol, Centella asiatica leaf extract and Tremella fuciformis extract; Phase C: niacinamide, bosaicin, peptides, bifida yeast and 1,2-hexanediol; Step 2: Add water, 1,3-butylene glycol, xanthan gum, high molecular weight sodium hyaluronate, low molecular weight sodium hyaluronate, oligomeric sodium hyaluronate, p-hydroxyacetophenone and glycerol polyether-26 into a beaker, heat to 80-85°C, stir until dissolved, then continue stirring for 30 minutes, cool to 45°C, and obtain a mixed solution A; Step 3: Add phase B into water to dissolve, then add to the mixed solution of step 2, add nicotinamide, bosaicin, peptide, bifida yeast and 1,2-hexanediol, continue stirring for 5 minutes to obtain a mixed solution B; Step 4: After the mixed solution B is left to stand for 1 hour, prepare the funnel, filter paper, glass rod and receiving container, fold the filter paper twice into a fan shape, unfold it into a cone shape, put it into the funnel, moisten the filter paper with a small amount of distilled water, ensure that the filter paper and the funnel fit tightly, carefully pour the upper clear liquid slowly into the funnel along the glass rod against the side with more filter paper layers, not exceeding two-thirds of the filter paper, wash the precipitate with a small amount of washing liquid and stir and settle it thoroughly, repeat more than three times, transfer it to the filter paper each time, and finally wash the container containing the precipitate three times, transfer it to the funnel each time, take the supernatant, and then add the adsorption clarifier to the supernatant, and finally filter it through a microporous filter membrane to obtain an anti-aging product; Step 5: Sterilize the anti-aging products and pack them into packaging containers according to the specified capacity.

4. The method for preparing an anti-aging product according to claim 1, characterized in that: The anti-aging product is anti-aging essence water.

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