Particle detection device and sample analyzer

Abstract

A particle detection device (3) and a sample analyzer, the particle detection device (3) comprising a light source (700), a front light assembly (200) comprising a single front light assembly (210), the single front light assembly (210) comprising a front light channel (211), the main body assembly (100) having a main body channel (110) wherein the cross section of the main body channel (110) is circular, a flow chamber assembly (400) comprising a flow chamber assembly (400) and a rear light assembly (300), the flow chamber assembly (400) comprising a flow chamber assembly (400) and a flow chamber assembly (400) comprising a flow chamber (400). The single front lens (210) is at least partially located in the main body channel (110) so as to be in nested connection with the main body lens (100); or, the cross section of the front light channel (211) is circular, and the main body lens (100) is partially located in the front light channel (211) so as to be connected with the single front light lens (210) in a nested mode. The front lens (210) is connected with the main lens (100) in a nested manner, so that the displacement of the front lens (210) and the main lens (100) in a radial plane is limited by the mutually abutted walls, and accurate positioning in the radial direction can be realized only by ensuring the precision of the inner circle and the outer circle of the front lens (210) and the main lens (100).

Description

Particle detection device and sample analyzer

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application is based on the Chinese patent application with application number 202221733952.0 and application date of July 6, 2022, and claims the priority of the Chinese patent application. The entire content of the Chinese patent application is hereby introduced into this application as a reference. Technical Field

[0003] The present invention relates to the field of medical equipment, and in particular to a particle detection device and a sample analyzer. Background Art

[0004] Common particle detection devices (such as cell detection devices) are devices that classify and count particles based on flow cytometry technology. The basic measurement principle is as follows: After being treated with reagents, sample particles (such as blood cells) are wrapped in sheath fluid and then, driven by pressure, pass through a flow chamber one by one. Laser light emitted by the light emitting component irradiates the sample particles in the flow chamber, causing scattering. The laser then passes through the light receiving device to form a variety of signals, such as scattered light signals, fluorescence signals, and light absorption signals, which are then used to classify and count different types of particles. To achieve accurate particle or cell detection, it is generally necessary to ensure that the laser excitation module, flow chamber module, and signal collection module have high optical alignment accuracy. Currently, particle detection devices on the market generally connect each module to a baseplate via a sliding mechanism, requiring individual module adjustment and multiple debugging dimensions. This leads to high production costs and low production efficiency.

[0005] Summary of the Invention

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a particle detection device that can reduce debugging costs and improve debugging efficiency.

[0007] The present invention also provides a sample analyzer using the above-mentioned particle detection device.

[0008] The particle detection device according to the first embodiment of the present invention includes:

[0009] a light source for providing irradiation light;

[0010] A front light assembly includes a single front light barrel and a front light optical module, wherein the single front light barrel includes a front light channel, the front light optical module is disposed in the front light channel, the irradiation light passes through the front light optical module, and the front light optical module shapes the irradiation light;

[0011] a main body lens barrel having a main body channel, through which the illumination light shaped by the front light optical module passes;

[0012] a flow cell assembly connected to the main lens barrel, the flow cell assembly having a detection portion for allowing a sample liquid to pass therethrough, the detection portion including an incident side disposed toward the front light assembly and an exit side disposed opposite the incident side, the illumination light shaped by the front light optical module entering the detection portion from the incident side and exiting from the exit side after irradiating the sample liquid to be tested;

[0013] a backlight assembly, comprising a backlight optical module, the backlight optical module shaping the illumination light emitted from the emission side of the detection portion;

[0014] a light receiving component for receiving the illumination light shaped by the rear light component;

[0015] The cross section of the main body channel is circular, and the single front light barrel is at least partially located in the main body channel so as to be nested and connected with the main body barrel;

[0016] Alternatively, the cross section of the front light channel is circular, and the main body lens barrel portion is located in the front light channel to be nested and connected with the single front light lens barrel;

[0017] The central axis of the front light channel, the central axis of the main body channel and the optical axis of the front light optical module are substantially coaxial.

[0018] The sample extraction device according to the embodiment of the present invention has at least the following beneficial effects:

[0019] The front light lens barrel and the main body lens barrel are nested and connected. In this way, the displacement between the front light lens barrel and the main body lens barrel in the radial plane is limited by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the inner and outer circles of the front light lens barrel and the main body lens barrel to achieve precise radial positioning.

[0020] In other embodiments of the present invention, the light source and the main body barrel can move relative to each other along the axial direction of the main body channel to adjust the distance between the light source and the flow chamber assembly.

[0021] In other embodiments of the present invention, the light source is connected to the single front light barrel, wherein:

[0022] The single front light lens barrel, the light source and the front light optical module can be synchronously moved relative to the main body lens barrel along the axial direction of the main body channel;

[0023] Alternatively, the light source is movable relative to the single front light barrel along the axial direction of the main body channel.

[0024] In other embodiments of the present invention, the particle detection device further includes a limit member, which is connected to one of the single front light lens barrel and the main body lens barrel, and can act on the other of the two to maintain the relative position between the single front light lens barrel and the main body lens barrel along the axial direction of the main channel, and / or to limit the relative rotation between the single front light lens barrel and the main body lens barrel along the circumference of the main channel or the circumference of the front light channel.

[0025] In other embodiments of the present invention, the other of the single front light barrel and the main body barrel has a limit groove, and the distance between the two side walls of the limit groove gradually decreases along the radial direction pointing to the central axis of the main body channel. The limit member is inserted into the limit groove, and the limit member abuts against the two side walls of the limit groove.

[0026] In other embodiments of the present invention, the backlight optical module includes a first condensing lens and a first aperture, and the light receiving component is used to receive the irradiation light emitted from the exit side of the detection unit and passing through the first condensing lens and the first aperture;

[0027] The particle detection device further includes a pressing member connected to the main body barrel, and the first aperture is clamped between the main body barrel and the pressing member.

[0028] In other embodiments of the present invention, the first condensing lens is connected to the main barrel and is located between the detection portion and the first aperture.

[0029] In other embodiments of the present invention, the pressing member is configured as a rear light barrel, the first condensing lens and the light receiving assembly are both connected to the rear light barrel, and the first condensing lens is located between the first aperture and the light receiving assembly.

[0030] In other embodiments of the present invention, the pressing member is configured as a rear light lens barrel, and the rear light lens barrel has at least a first rear light channel, wherein:

[0031] When the cross section of the main body channel is circular, the rear light barrel is at least partially located in the main body channel to be nested and connected with the main body barrel;

[0032] Alternatively, the cross section of the first rear light channel is circular, and the main body lens barrel is partially located in the first rear light channel to be nested and connected with the rear light lens barrel;

[0033] The first rear light channel is used to pass the illumination light emitted from the exit side of the detection part, and the central axis of the first rear light channel, the central axis of the main channel, the central axis of the front light channel and the optical axis of the front light optical module are roughly coaxial.

[0034] In other embodiments of the present invention, the main channel includes a first channel section and a second channel section, and the first channel section and the second channel section are arranged in sequence along the axial direction of the main channel. The diameter of the first channel section is smaller than the diameter of the second channel section to form an abutment surface at the intersection of the two, and the clamping member is inserted into the second channel section to press the first aperture against the abutment surface.

[0035] In other embodiments of the present invention, a wall of the second channel section is provided with a first hole penetrating to the outer surface of the main body barrel, and the first hole is used for allowing the first aperture to move relative to the main body barrel along the penetrating direction of the first hole.

[0036] In other embodiments of the present invention, the pressing member is detachably connected to the main barrel, and when the pressing member is in a non-pressing state, the first aperture is configured to be movable relative to the main barrel along a first direction, and the first direction is parallel to the radial direction of the main channel perpendicular to the flow direction of the sample liquid to be tested.

[0037] In another embodiment of the present invention, one of the main barrel and the first aperture is provided with a first positioning portion, and the other is provided with a second positioning portion, and the first aperture can be moved along the first direction by the first positioning portion and the second positioning portion;

[0038] Alternatively, one of the pressing member and the first aperture is provided with a first positioning portion, and the other is provided with a second positioning portion, and the first aperture can be moved along the first direction through the first positioning portion and the second positioning portion.

[0039] In other embodiments of the present invention, the rear light assembly further includes a rear light barrel, the rear light barrel having at least a first rear light channel, the central axis of the first rear light channel, the central axis of the main body channel, the central axis of the front light channel, and the optical axis of the front light optical module being substantially coaxial, the rear light optical module including a first condensing lens and a first aperture, the light receiving assembly being configured to receive the irradiation light emitted from the exit side of the detection portion and passing through the first condensing lens and the first aperture, wherein the first condensing lens and the first aperture are disposed in the first rear light channel, and the light receiving assemblies are both connected to the rear light barrel, wherein:

[0040] When the cross section of the main body channel is circular, the rear light barrel is at least partially located in the main body channel to be nested and connected with the main body barrel;

[0041] Alternatively, the cross section of the first rear light channel is circular, and the main body lens barrel portion is located in the first rear light channel to be nested and connected with the rear light lens barrel.

[0042] In other embodiments of the present invention, the rear light optical module includes a first focusing lens and a first aperture, and the light receiving component is used to receive the irradiation light emitted from the exit side of the detection unit and passing through the first focusing lens and the first aperture, wherein the first focusing lens, the first aperture and the light receiving component are all connected to the main lens barrel.

[0043] In other embodiments of the present invention, the wall of the main channel is provided with a first hole penetrating to the outer surface of the main barrel, and the first aperture is configured to be movable relative to the main barrel along a penetrating direction of the first hole through the first hole.

[0044] In other embodiments of the present invention, at least one end of the first aperture extends from the first hole, and the particle detection device further includes a fixing member, which acts on the extending portion of the first aperture to fix the first aperture to the main barrel.

[0045] In other embodiments of the present invention, the rear light assembly further includes a rear light barrel, the rear light barrel having at least a first rear light channel, the first rear light channel being used to pass the illumination light emitted by the exit side of the detection portion, the central axis of the first rear light channel, the central axis of the main body channel, the central axis of the front light channel, and the optical axis of the front light optical module being substantially coaxial, and the rear light optical module being at least partially connected to the rear light barrel;

[0046] Alternatively, the rear light assembly includes a rear light optical module, and all of the rear light optical modules are connected to the main body barrel.

[0047] In other embodiments of the present invention, the flow chamber assembly further includes an injection portion and a discharge portion, wherein the injection portion, the detection portion and the discharge portion are sequentially arranged along the flow direction of the sample liquid to be tested, wherein at least the detection portion is located in the main channel.

[0048] In other embodiments of the present invention, the flow chamber assembly is connected to the main barrel and is configured to be movable relative to the main barrel along a first direction, wherein the first direction is parallel to a radial direction of the main channel perpendicular to the flow direction of the sample liquid.

[0049] In other embodiments of the present invention, one of the main barrel and the flow chamber assembly is provided with a third positioning portion, and the other is provided with a fourth positioning portion, and the flow chamber assembly can move relative to the main barrel along the first direction through the third positioning portion and the fourth positioning portion.

[0050] In other embodiments of the present invention, the backlight optical module includes a first condensing lens and a reflector, and the light receiving assembly includes a first light receiving device and a second light receiving device;

[0051] The first condenser lens is configured to converge first illumination light emitted from the exit side of the detection unit and within a first angle range, second illumination light emitted from the exit side of the detection unit and within a second angle range, and third illumination light emitted from the exit side of the detection unit and within a third angle range;

[0052] The reflector is used to reflect the light formed by the second illumination light after being converged by the first condensing lens;

[0053] The first light receiving device is used to receive light formed by the first illumination light being converged by the first condensing lens, and light formed by the third illumination light being converged by the first condensing lens, and the second light receiving device is used to receive light formed by the second illumination light being converged by the first condensing lens and reflected by the reflector.

[0054] In other embodiments of the present invention, each angle in the second angle range is smaller than each angle in the first angle range, and each angle in the third angle range is larger than each angle in the second angle range and smaller than each angle in the first angle range.

[0055] In other embodiments of the present invention, the first light receiving device and the second light receiving device both have a light receiving area, the light receiving areas of the first light receiving device and the second light receiving device are oriented in the same direction, and along the axial direction of the main channel, the distance between the light receiving area of ​​the first light receiving device and the first focusing lens is smaller than the distance between the light receiving area of ​​the second light receiving device and the first focusing lens.

[0056] In other embodiments of the present invention, the front light optical module includes a cylindrical lens and a second condensing lens sequentially arranged along the axial direction of the front light channel, and the cylindrical lens and the second condensing lens are located in the front light channel.

[0057] In other embodiments of the present invention, the front-light optical module further includes an aspheric lens, and the aspheric lens is located in the front-light channel and between the light source and the cylindrical lens.

[0058] In other embodiments of the present invention, the front light optical module further includes an optical isolator, which is located in the front light channel and between the aspheric lens and the cylindrical lens;

[0059] And / or, the front light optical module further includes a second aperture, which is located in the front light channel and between the light source and the aspheric lens.

[0060] In other embodiments of the present invention, along the axial direction of the front light channel, the front light channel includes a plurality of first mounting cavities sequentially arranged and extending to the rear end surface of the front light lens barrel, and the inner diameter of each first mounting cavity increases sequentially.

[0061] In other embodiments of the present invention, the front light channel further includes a second mounting cavity, the second mounting cavity extends to the front end surface of the front light barrel, and the light source is at least partially located in the second mounting cavity.

[0062] A sample analyzer according to a fifth embodiment of the present invention includes:

[0063] A sampling device for obtaining a sample to be tested;

[0064] a sample preparation device, configured to receive the sample to be tested obtained by the sampling device, and mix the sample to be tested with a reagent to prepare a sample liquid to be tested;

[0065] The particle detection device;

[0066] a liquid supply device for supplying sheath fluid to the flow cell assembly, so that particles in the sample liquid to be tested are carried by the sheath fluid and arranged in a queue in the detection portion;

[0067] The controller is configured to obtain a measurement result of the sample to be measured according to the feedback signal received by the light receiving component.

[0068] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0069] The present invention will be further described below with reference to the accompanying drawings and embodiments, in which:

[0070] FIG1 is a schematic diagram of a particle detection device in the related art;

[0071] FIG2 is a perspective schematic diagram of a particle detection device in one direction according to an embodiment of the present invention;

[0072] FIG3 is a perspective schematic diagram of the particle detection device in FIG2 from another direction;

[0073] FIG4 is a cross-sectional view of the particle detection device in FIG2 in an exploded state, wherein the cross-sectional plane in FIG4 is arranged vertically;

[0074] FIG5 is a cross-sectional view of the particle detection device in FIG2 in an assembled state, wherein the cross-sectional plane in FIG5 is arranged horizontally;

[0075] FIG6 is a perspective schematic diagram of the connection between the main lens barrel and the first aperture in FIG2 ;

[0076] FIG7 is a perspective schematic diagram of the liquid injection portion in FIG2 ;

[0077] FIG8 is a perspective schematic diagram of a hidden flow chamber assembly of the particle detection device in FIG2 ;

[0078] FIG9 is a schematic diagram of the optical path of the particle detection device in FIG2 ;

[0079] FIG10 is a schematic three-dimensional diagram of a sample analyzer according to an embodiment of the present invention.

[0080] Reference numerals:

[0081] Sampling device 1, sample preparation device 2, particle detection device 3, display device 4, control device 5, first housing 6, second housing 7;

[0082] Front light module 10, flow chamber module 20, signal collection module 30, detector module 40, substrate 50, sliding mechanism 60;

[0083] Main body barrel 100, main body channel 110, first channel section 111, second channel section 112, abutting surface 113, first hole 120, first positioning portion 130, second hole 140, second positioning surface 150, threaded hole 151, fourth positioning surface 160, front light assembly 200, front light barrel 210, front light channel 211, limiting groove 212, first mounting cavity 213, second mounting cavity 214, second aperture 220, cylindrical lens 230, second condensing lens 240, aspheric lens 250, optical isolator 260, rear light assembly 300, rear light barrel 310, first rear light channel 311, second rear light channel 3 12. First focusing lens 320, first aperture 330, second positioning portion 331, reflecting mirror 340, third aperture 350, fourth aperture 360, third focusing lens 370, flow chamber assembly 400, liquid injection portion 410, first positioning surface 411, third positioning surface 412, first mounting portion 413, second mounting portion 414, connecting hole 415, flange 416, boss 417, detection portion 420, liquid discharge portion 430, limit member 500, support member 600, light source 700, first light receiving device 810, second light receiving device 820, first irradiation light L1, second irradiation light L2, third irradiation light L3. DETAILED DESCRIPTION

[0084] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0085] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0086] In the description of the present invention, "several" means more than one, "plurality" means more than two, "greater than," "less than," and "exceed" are understood to exclude the number itself, while "above," "below," and "within" are understood to include the number itself. The use of "first" and "second" in the description is solely for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, implicitly specifying the number of the indicated technical features, or implicitly specifying the order of the indicated technical features.

[0087] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, and connecting should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0088] In the description of the present invention, reference to terms such as "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the exemplary expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0089] The basic principle of a particle detection device is to surround a sample liquid containing particles to be detected with a sheath liquid, so that the particles to be detected are arranged in a single row and pass through the detection area of ​​the flow chamber in sequence. The particles to be detected flowing through the detection area are then irradiated with a laser, and the scattered light and / or fluorescence formed after the irradiation is received by a light receiving device. Based on the scattered light signal and / or fluorescence signal, relevant particle parameters can be further obtained. Referring to Figure 1, a typical particle detection device is shown, which includes a front light module 10, a flow chamber module 20, a signal collection module 30, a detector module 40, a substrate 50 and a plurality of sliding mechanisms 60. The sample liquid to be detected flows through the flow chamber module 20 in a vertical direction. The light emitted by the front light module 10 is irradiated on the detection area of ​​the flow chamber module 20. The light emitted from the flow chamber module 20 is collected by the signal collection module 30 and finally received by the detector module 40. Since the size of the particles to be tested is usually very small, the accuracy of the entire optical path is required to be very high. In order to ensure the accuracy of the optical path, the particle detection device in Figure 1 connects the front light module 10, the flow chamber module 20, the signal collection module 30, and the detector module 40 to the base plate 50 through a sliding mechanism 60. During the assembly process, the operator uses the sliding mechanism 60 to debug the positions of each module relative to each other. Since there are a large number of modules and the modules involve movement in at least two dimensions, the overall debugging steps are very complicated, which significantly increases the production cost.

[0090] Based on the above problems, the present invention proposes a particle detection device, which connects at least two modules by using a main lens barrel 100 as a medium, and ensures the accuracy of the optical path through the processing accuracy of the main lens barrel 100 and the assembly accuracy of the main lens barrel 100 and other modules, thereby significantly reducing the difficulty of debugging and reducing costs. Different assembly schemes will be explained below in conjunction with the accompanying drawings and different embodiments.

[0091] For the convenience of description, the nouns appearing in the present invention are first explained:

[0092] The “flow direction of the sample liquid to be tested” refers to the flow direction of the sample liquid containing the particles to be tested in the flow chamber. When the particle detection device is placed on a horizontal plane as shown in FIG4 , the “flow direction of the sample liquid to be tested” can be understood as the direction from bottom to top in FIG4 .

[0093] The "axial direction of the main channel" refers to the direction in which the central axis of the main channel extends. When the particle detection device is placed on a horizontal surface as shown in Figure 4, the "axial direction of the main channel" can be understood as the direction from front to back or from back to front. In some places, the "axial direction of the main channel" is simply expressed as the axial direction.

[0094] The “radial direction of the main channel perpendicular to the flow direction of the sample liquid to be tested” refers to the diameter direction of any cross-section of the main channel that is perpendicular to the flow direction of the sample liquid to be tested. When the particle detection device is placed on a horizontal plane as shown in FIG5 , the “radial direction of the main channel perpendicular to the flow direction of the sample liquid to be tested” can be understood as a direction passing through the central axis of the main channel and from left to right or from right to left.

[0095] The "first direction" refers to a direction parallel to the "radial direction of the main channel perpendicular to the flow direction of the sample liquid to be tested". In other words, when referring to the first direction, it is not required to pass through the central axis of the main channel. When the particle detection device is placed on a horizontal plane as shown in Figure 5, the "first direction" can be understood as a direction from left to right or from right to left.

[0096] "Approximately coaxial" means that the central axes, the central axes and the optical axes, and the central axes and the optical axes are completely coaxial or within the allowable error range, which specifically includes two situations: one is that the axes are parallel to each other and the distance is within a preset range, and the preset range includes 0, that is, the axes can either coincide or deviate by a certain distance; the other is that the axes intersect and the angle between them is within a preset range.

[0097] 2 to 5 , a perspective schematic diagram and a cross-sectional view of a particle detection device according to an embodiment of the present invention are shown, respectively. The cross-sectional plane in FIG4 is a vertical plane passing through the axis of the main lens barrel 100, and the modules in FIG4 are in an exploded state. The cross-sectional plane in FIG5 is a horizontal plane passing through the axis of the main lens barrel 100. The particle detection device primarily comprises the main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow cell assembly 400, a light source 700, and a light receiving assembly. The light source 700 is configured to provide illumination light. The flow cell assembly 400 has a flow channel therein through which a sample liquid to be tested passes. The front light assembly 200 is configured to shape the illumination light emitted from the light source 700 and incident on the flow cell assembly 400. The rear light assembly 300 is configured to shape the illumination light emitted from the flow cell assembly 400. The light receiving assembly is configured to receive the illumination light shaped by the rear light assembly.

[0098] The front light assembly 200 includes a front light optical module, which includes an optical shaping element. Light from the light source 700 is shaped by the shaping element before irradiating the flow cell assembly 400, forming a light spot on the detection section 420 of the flow cell assembly 400. The back light assembly 300 includes a back light optical module, which includes a first condenser lens and a first aperture component. Light emitted from the flow cell assembly 400 passes through the first condenser lens and the first aperture before being received by a light receiving assembly. The light receiving assembly includes a light receiving device for receiving optical signals and converting them into electrical signals.

[0099] The flow chamber assembly 400 includes a detection section 420 for allowing the sample liquid to pass through. The detection section 420 is made of a light-transmitting material such as quartz glass, and has a flow channel extending in a vertical direction inside. The sample liquid to be tested and the sheath fluid surrounding it flow through the flow channel. The detection section 420 has an incident side (e.g., the front side) arranged toward the front light assembly 200 and an exit side (e.g., the rear side) arranged opposite the incident side. The irradiation light shaped by the front light optical module enters the detection section from the incident side and exits from the exit side after irradiating the sample liquid to be tested. In general, the light emitted by the light source 700 is shaped by the front light optical module and then enters the detection section 420 from the incident side. After irradiating the sample liquid to be tested, it exits from the exit side, is shaped by the rear light assembly 300, and is then received by the light receiving assembly.

[0100] The main lens barrel 100 serves as the primary mounting structure for mounting two or more modules. The main lens barrel 100 has a main channel 110 for transmitting light emitted by the front light assembly 200. By nesting the main lens barrel 100 with other structures, such as the front light barrel and the rear light barrel, as a connecting medium, the alignment of the optical path can be ensured by utilizing the mutual coordination accuracy between the lens barrels, thereby greatly reducing the difficulty and cost of debugging. In some embodiments, as shown in Figures 2 to 5, the main lens barrel is an entire cylindrical structure, which facilitates processing and further ensures processing accuracy.

[0101] Based on the above structure, the front light lens barrel and the main body lens barrel are nested and connected as a basic solution. Referring to Figures 4 and 5, the front light assembly 200 of the first embodiment of the present invention also includes a front light lens barrel 210, which has a front light channel 211. The front light optical module is arranged in the front light channel 211. The irradiation light passes through the front light optical module and is shaped by the front light optical module. In this embodiment, the light source 700 and the front light optical module are both connected to the front light lens barrel 210. In other embodiments, the front light optical module is connected to the front light lens barrel 210. The front light lens barrel 210 is nested and connected to the main body lens barrel 100. After the front light lens barrel 210 is nested and connected to the main body lens barrel 100, the central axis of the front light channel 211, the central axis of the main body channel 110, and the optical axis of the front light optical module are roughly coaxial.

[0102] There are two solutions for the nested connection between the front light barrel 210 and the main body barrel 100:

[0103] In one embodiment, the cross section of the main channel 110 is the center of a circle, and the front light lens barrel 210 is at least partially located within the main channel 110. In other words, this embodiment is a case where the front light lens barrel 210 is plugged into the main channel 110. Taking FIG5 as an example, the rear end of the front light lens barrel 210 is plugged into the front end of the main channel 110. In other embodiments, the front light lens barrel 210 is completely plugged into the main channel 110. In order to adapt to the plug-in fit, the outer contour of the plug-in portion of the front light lens barrel 210 is set to be circular and adapted to the shape and size of the main channel 110. In the illustrated embodiment, the front light lens barrel 210 is set as a cylindrical structure as a whole. After the two are assembled, the displacement between the front light lens barrel 210 and the main body lens barrel 100 in the radial plane is limited by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the outer circle of the front light lens barrel 210 and the inner circle of the main body lens barrel 100 to achieve the centering of the lens barrel. Combined with the processing and assembly accuracy, the precise installation between the optical device and the lens barrel is guaranteed, so that the irradiation light emitted after the front light component 200 is shaped and accurately illuminates the particles passing through the detection part 420, which can simplify the structure and significantly reduce the debugging steps and debugging costs. It can be understood that in this solution, the cross-sections of the main channel 110 and the insertion part of the front light lens barrel 210 are both circular. Compared with other cavities and insertion parts with non-circular cross-sections (such as polygonal cavities including rectangles, or special-shaped cavities with inner walls including planes and arcs), higher processing accuracy can be guaranteed through relatively simple processes. For example, a high-precision circular channel or circular outer contour can be obtained by rotating the blank with a tool.

[0104] In another scheme, the cross-section of the front light channel 211 is circular, and the main body lens barrel 100 is partially located in the front light channel 211. In other words, this scheme is a situation in which the main body lens barrel 100 is plugged into the front light channel 210. For example, the front end of the main body lens barrel 100 is plugged into the rear end of the front light channel 211. In order to adapt to the plug-in fit, the outer contour of the plug-in portion of the main body lens barrel 100 is set to a circular shape and adapted to the shape and size of the front light channel 211. For example, the main body lens barrel 100 is set to a cylindrical structure as a whole. Similarly, this scheme can also serve the purpose of simplifying the structure, reducing the debugging steps and debugging costs. It should be noted that the cross-sectional shape of the main body channel 110 in this scheme is not limited to a circle.

[0105] In addition, in this embodiment, the front light assembly 200 includes a single front light lens barrel 210, and the main lens barrel 100 is nested and connected with the single front light lens barrel 210. At least the front light optical module is indirectly connected to the main lens barrel 100 through the front light lens barrel 210. In other words, the front light portion of this embodiment has one and only one lens barrel directly nested and connected with the main lens barrel 100, and the lens barrel directly nested and connected with the main lens barrel 100 is the front light lens barrel 210 of this embodiment. The front light optical module includes a plurality of lenses. In some embodiments, the plurality of lenses are directly connected to the front light lens barrel 210. The positions of the plurality of lenses rely on the processing accuracy and assembly accuracy of the front light lens barrel 210 to ensure that the optical axes are roughly coaxial. Its structure is relatively simple, and the number of components can be reduced. In other embodiments, the front light optical module further includes at least one secondary lens barrel, at least part of the plurality of lenses is first connected to the secondary lens barrel, and then connected to the front light lens barrel 210 through the secondary lens barrel, and the plurality of lenses are guaranteed by the processing accuracy and assembly accuracy of the front light lens barrel 210 and the secondary lens barrel, so that modular assembly of the front light optical module can be achieved. It should be noted that even when the front light optical module has a secondary lens barrel, it is still only the front light lens barrel 210 that is nested and connected to the main lens barrel 100, and the secondary lens barrels are indirectly connected to the main lens barrel 100 through the front light lens barrel 210. In this way, the front light assembly 200 can be pre-assembled separately, and then the front light lens barrel 210 is nested and connected to the main lens barrel 100 for easy assembly.

[0106] Based on the aforementioned nested connection between the front light lens barrel 210 and the main body lens barrel 100, in some embodiments, the light source 700 and the main body lens barrel 100 can move relative to each other along the axial direction of the main body channel 110 to adjust the distance between the light source 700 and the flow chamber assembly 400. Specifically, when the light source 700 is connected to the front light lens barrel 210, the front light lens barrel 210, the light source 700, and the front light optical module can move synchronously relative to the main body lens barrel 100. In addition, the front light lens barrel 210 can remain fixed relative to the main body lens barrel 100, and the light source 700 can move independently relative to the front light lens barrel 210 (main body lens barrel 100), for example, the light source 700 is slidably connected to the front light lens barrel 210 through a secondary lens barrel. When the light source 700 is not directly connected to the front light lens barrel 210, the front light lens barrel 210 remains fixed relative to the main body lens barrel 100, and the light source 700 can move independently relative to the front light lens barrel 210 (main body lens barrel 100). 5 , for example, the light source 700 is connected to the front light barrel 210, and a certain external force can be applied to the front light barrel 210 to overcome the friction between the two, thereby driving the front light barrel 210 to slide within the main barrel 100. In other embodiments, an axial screw or other structure can be provided between the front light barrel 210 and the main barrel 100, and the rotation of the screw can drive the relative movement between the two barrels.

[0107] When the front light lens barrel 210 and the main body lens barrel 100 can move relative to each other, the particle detection device in some embodiments also includes a stopper 500, which is connected to one of the front light lens barrel 210 and the main body lens barrel 100 and can act on the other of the two to maintain the relative position along the axial direction after the two move, so as to avoid the front light lens barrel 210 and the main body lens barrel 100 from moving relative to each other after adjustment. As shown in Figure 4, the stopper 500 can be an elastic pin, which includes a cylindrical main body, an elastic member is provided in the main body, and the end of the main body is provided with a roller, and the elastic member and the ball are abutted. The barrel wall of the main body lens barrel 100 is provided with a radial mounting hole, and the stopper 500 is located in the mounting hole, and the roller abuts the outer wall of the front light lens barrel 210. Like this, the debugging personnel can easily move the front light lens barrel 210 during the debugging process, and after moving, the stopper 500 can increase the friction between the front light lens barrel 210 and the main body lens barrel 100 so that the two will not move at will. In other embodiments, the limiting member 500 may be a magnet, and the front light barrel 210 is made of ferromagnetic material, and the relative axial position between the barrels is maintained by magnetic adsorption.

[0108] It is understandable that when the main lens barrel 100 is inserted into the front light lens barrel 210 , the stopper 500 is fixed to the front light lens barrel 210 and abuts against the outer wall of the main lens barrel 100 .

[0109] It should be noted that the limiting member 500 in this embodiment is used for the initial fixation of the lens barrel during the assembly process. After the debugging is completed, the main lens barrel 100 and the front light lens barrel 210 can be fixed by adhesives or threaded fasteners.

[0110] When the front light lens barrel 210 and the main body lens barrel 100 are capable of relative movement, the particle detection device in some embodiments further includes a stopper 500, which is connected to one of the front light lens barrel 210 and the main body lens barrel 100 and can act on the other of the two to limit the relative rotation along the circumferential direction between the front light lens barrel 210 and the main body lens barrel 100. For example, taking the front light lens barrel 210 as an example of being plugged into the main body lens barrel 100, the stopper 500 is the aforementioned pin or screw, and is fixed to the main body lens barrel 100, the outer wall surface of the front light lens barrel 210 is provided with a flat surface, and the pin or screw abuts against the flat surface, or the outer wall surface of the front light lens barrel 210 is provided with a groove, and the pin or screw is inserted into the groove.

[0111] Specifically, in some embodiments, the other of the front light lens barrel 210 and the main body lens barrel 100 has a limiting groove 212, and the limiting member 500 is inserted into the limiting groove 212 to limit circumferential rotation. At the same time, the distance between the groove walls of the limiting groove 212 gradually decreases along the radial direction pointing to the central axis of the main body channel 110. The limiting member 500 abuts against the groove walls of the limiting groove 212, and can also achieve centering of the front light lens barrel 210 and the main body lens barrel 100. With specific reference to Figure 4, the limiting groove 212 is provided on the outer wall of the front light lens barrel 210 and extends along the axial direction of the lens barrel. The cross-section of the limiting groove 212 is set to V-shaped for easy processing.

[0112] Based on the aforementioned nested connection between the front light barrel 210 and the main body barrel 100, the main body barrel 100 can also be used to connect at least some of the components in the rear light assembly 300. Referring to Figures 4 and 5, in some embodiments, the rear light assembly 300 includes a rear light optical module, which includes a first condenser lens 320 and a first aperture 330. The first condenser lens 320 is used to converge light, thereby reducing the light spot irradiated on the light receiving device, thereby reducing the target surface area of ​​the light receiving device, and ultimately reducing the volume and cost of the light receiving device. At least one of the incident surface and the exit surface of the first condenser lens 320 is an aspherical surface. In this embodiment, the first condenser lens 320 adopts an aspherical lens, which is conducive to better light collection. The first aperture 330 is used to block stray light irradiated on the light receiving device, thereby improving the quality of the optical signal collected by the light receiving device and helping to improve the optical signal-to-noise ratio. The light receiving device may be a photodetector, configured to receive the illumination light emitted from the flow chamber assembly 400 and passing through the first condensing lens 320 and the first aperture 330 .

[0113] In this embodiment, the main lens barrel 100 is also used to mount at least the first aperture 330. Specifically, the particle detection device further includes a clamping member connected to the main lens barrel 100, thereby clamping the first aperture 330 between the main lens barrel 100 and the clamping member, further enhancing the integrated installation function of the main lens barrel 100. In addition, compared with fixing methods using adhesives or threaded fasteners, the clamping method can be more convenient for assembly and disassembly. It should be noted that the clamping member can be the rear light lens barrel of the rear light assembly 300, or it can be another separately designed structure.

[0114] With the first aperture clamped between the main lens barrel 100 and the pressing member, referring to FIG5 , in some embodiments, the first condenser lens 320 is connected to the main lens barrel 100 and positioned between the flow chamber assembly 400 and the first aperture 330. In this manner, the illumination light emitted from the flow chamber assembly 400 is first converged by the first condenser lens 320 and then passes through the first aperture 330, thereby reducing the area of ​​the opening in the first aperture 330. Furthermore, using the main lens barrel 100 as the mounting base for installing the first condenser lens 320 of the rear light optical module allows both the first condenser lens 320 and the front light assembly 200 to use the main lens barrel 100 as a reference, facilitating alignment of the optical path. Furthermore, the machining precision of the main lens barrel 100 can be leveraged to ensure assembly accuracy, further reducing the number of debugging steps.

[0115] On the basis of the first aperture being clamped between the main body barrel 100 and the pressing member, the pressing member in some embodiments is inserted into the main body channel 110 to compress the first aperture 330. Referring to Figures 4 and 5, the main body channel 110 includes a first channel section 111 and a second channel section 112. The first channel section 111 and the second channel section 112 are arranged in sequence along the axial direction of the main body channel 110, that is, the first channel section 111 is located to the left of the second channel section 112 in the figure. The diameter of the first channel section 111 is smaller than that of the second channel section 112, so as to form an abutment surface 113 at the intersection of the two. The front end of the pressing member is inserted into the second channel section 112 to press the first aperture 330 against the abutment surface 113. In this way, the pressing member and the main body barrel 100 are connected in a plug-in manner, which can simplify the assembly structure between the pressing member and the main body barrel 100 and facilitate assembly.

[0116] Specifically in the illustrated embodiment, the first channel section 111 can also be used to assemble the first condenser lens 320. It should be noted that in addition to the first channel section 111 and the second channel section 112, the main channel 110 can also be provided with other channel sections, such as a third channel section provided on the left side of the first channel section 111 for inserting the front light barrel 210.

[0117] In some specific embodiments, referring to Figures 2, 3, and 5, the wall of the second channel section 112 is provided with a first hole 120 extending through the outer surface of the main lens barrel 100. The first hole 120 is used to allow the first aperture 330 to move relative to the main lens barrel 100 along the direction of the first hole 120, which is the aforementioned first direction. The first hole 120 is provided on one or both opposing sides of the wall of the second channel section 112, and the movement direction of the first aperture 330 is specifically a front-to-back direction. Movement in the aforementioned direction allows the first aperture 330 to be moved in and out of the main lens barrel 100, thereby enabling assembly of the first aperture 330. Furthermore, the position of the first aperture 330 can be fine-tuned after it is positioned between the main lens barrel 100 and the pressing member, thereby adjusting the position between the first aperture 330 and the first focusing lens 320. It should be noted that since the first aperture 330 can be moved laterally relative to the main lens barrel 100, when assembling or adjusting the first aperture 330, the clamping part does not need to be completely withdrawn from the main channel 110, but only needs to be moved a short distance backward relative to the main lens barrel 100, which is convenient for operation.

[0118] Based on the first aperture being clamped between the main lens barrel 100 and the pressing member, in some embodiments, the pressing member is detachably connected to the main lens barrel 100, and when the pressing member is in a non-pressed state, the first aperture 330 is configured to be movable relative to the main lens barrel 100 in the aforementioned first direction, thereby enabling installation or adjustment of the first aperture 330. The pressing member of this embodiment can be detachably connected to the main lens barrel 100 using the aforementioned plug-in fitting method, or can be detachably connected to the main lens barrel 100 using threaded fasteners or the like.

[0119] It should be noted that the “non-pressing state” referred to here includes both a state in which the pressing member is completely separated from the main channel 110 and a state in which there is a gap between the pressing member and the abutting surface 113 to allow the first aperture 330 to move.

[0120] To position the first aperture 330 during movement, referring to Figures 4 and 6 , in some embodiments, one of the main barrel 100 and the first aperture 330 is provided with a first positioning portion 130, and the other is provided with a second positioning portion 331. The first aperture 330 can be moved relative to the main barrel 100 in a first direction (e.g., left-right in Figure 6 ) via the first positioning portion 130 and the second positioning portion 331. In some specific embodiments, the first positioning portion 130 includes a positioning protrusion extending parallel to the axial direction of the main channel 110, such as a positioning post provided on the abutment surface 113. The second positioning portion 331 includes a positioning recess extending in the first direction, such as a strip-shaped positioning groove provided on the first aperture 330. The positioning protrusion is inserted into the positioning recess and can slide along the extending direction of the positioning recess for positioning. It is understood that the main barrel 100 can also be provided with a positioning groove, while the first aperture 330 is provided with a positioning post. Based on the aforementioned structure, the first aperture 330 of this embodiment only needs to move in the left-right direction, further reducing the debugging process.

[0121] It should be noted that when the abutment surface 113 is provided with a positioning post, a vertical reference plane parallel to the axial direction of the main channel 110 is established, and the projection of the first hole 120 in the reference plane should cover and be wider than the projection of the positioning post in the reference plane. In this way, the first aperture 330 can first be offset from the positioning post along the axial direction, enter the main channel 110 along the first direction, and then move axially toward the abutment surface 113 to allow the positioning post to be inserted into the positioning groove.

[0122] In other embodiments, one of the clamping member and the first aperture 330 may be provided with a first positioning portion 130, and the other may be provided with a second positioning portion 331, and the first aperture 330 may be able to move relative to the main lens barrel 100 along the first direction through the first positioning portion 130 and the second positioning portion 331.

[0123] On the basis of the aforementioned nested connection between the front light barrel 210 and the main body barrel 100, the optical devices of the rear light assembly 300 can also be installed in other positions. For example, the rear light assembly 300 in some embodiments includes a rear light optical module, and the rear light optical module includes the aforementioned first focusing lens 320 and first aperture 330, wherein the first focusing lens 320, the first aperture 330 and the light receiving assembly are all connected to the main body barrel 100. In this way, the first focusing lens 320, the first aperture 330, the light receiving assembly and the front light assembly 200 all use the main body barrel 100 as a reference, which is convenient for ensuring the centering of the optical path, and the assembly accuracy can be guaranteed by the processing accuracy of the main body barrel 100, which can further reduce the debugging steps.

[0124] In some specific embodiments, the wall of the second channel section 112 is provided with a first hole 120 extending through the outer surface of the main lens barrel 100. The first hole 120 is used to allow the first aperture 330 to move relative to the main lens barrel 100 in a through-direction, which is the aforementioned first direction. The first holes 120 are provided on one or two opposing walls of the second channel section 112, and the movement direction of the first aperture 330 is specifically a front-to-back direction. Movement in the aforementioned direction allows the first aperture 330 to be moved in and out of the main lens barrel 100, thereby enabling assembly of the first aperture 330. Furthermore, the position of the first aperture 330 can be fine-tuned after it is positioned between the main lens barrel 100 and the pressing member, thereby adjusting the position between the first aperture 330 and the first focusing lens 320. Since the first aperture 330 can be moved laterally from the main barrel 100 , it is not necessary to enter or exit the main barrel 100 from the rear end of the main barrel 100 . Therefore, when assembling or adjusting the first aperture 330 , it is not necessary to remove or move the optical device behind the first aperture 330 .

[0125] Based on the aforementioned structure, to secure the first aperture 330, at least one end of the first aperture 330 extends from the first hole 120. The particle detection apparatus further includes a fixing member that acts on the protruding portion of the first aperture 330 to secure the first aperture 330 to the main lens barrel 100. For example, the fixing member may be a threaded fastener. The first aperture 330 is provided with a through-hole, and the outer side of the main lens barrel 100 is provided with an axial threaded hole. The threaded fastener passes through the through-hole and screws into the threaded hole to secure the first aperture 330 to the main lens barrel 100. It will be understood that the walls of the main lens barrel 100 are provided with first holes 120 on opposite sides, and the two ends of the first aperture 330 extend from the corresponding first holes 120 and are respectively secured by fixing members, thereby achieving a stable connection of the first aperture 330.

[0126] The aforementioned embodiments are all described based on the nested connection between the front light lens barrel 210 and the main body lens barrel 100. The following description will be based on the nested connection between the rear light lens barrel 310 and the main body lens barrel 100. Referring also to Figures 4 and 5, the particle detection device in the second embodiment of the present invention includes the main body lens barrel 100, the front light assembly 200, the rear light assembly 300, the flow chamber assembly 400, the light source 700 and the light receiving assembly. The flow chamber assembly 400, the light source 700 and the light receiving assembly can be understood with reference to the first embodiment. The front light assembly 200 of this embodiment can also be understood with reference to the first embodiment. The front light lens barrel 210 can also be omitted, and the front light optical module can be directly connected to the main body lens barrel 100, or connected to the main body lens barrel 100 through other components different from the front light lens barrel 210.

[0127] The rear light assembly 300 includes a rear light barrel 310 and a rear light optical module. The rear light barrel 310 has at least a first rear light channel 311. The rear light optical module is at least partially disposed within the first rear light channel 311. The illumination light emitted from the exit side of the detection unit 420 passes through the rear light optical module, which then shapes the illumination light emitted from the exit side of the detection unit 420. The rear light barrel 310 is nested and connected to the main body barrel 100. When the rear light barrel 310 and the main body barrel 100 are nested and connected, the central axis of the first rear light channel 311, the central axis of the main body channel 110, and the optical axis of the front light optical module are substantially coaxial.

[0128] There are two solutions for the nested connection between the rear light barrel 310 and the main body barrel 100:

[0129] In one embodiment, the cross section of the main body channel 110 is the center of a circle, and the rear light lens barrel 310 is at least partially located within the main body channel 110. In other words, this embodiment is a case where the rear light lens barrel 310 is plugged into the main body channel 100. Taking FIG. 5 as an example, the rear end of the rear light lens barrel 310 is plugged into the front end of the main body channel 110. In other embodiments, the rear light lens barrel 310 is completely plugged into the main body channel 110. To accommodate the plug-in fit, the outer contour of the plug-in portion of the rear light lens barrel 310 is set to a circular shape and adapted to the shape and size of the main body channel 110. In the illustrated embodiment, the front end of the rear light lens barrel 310 is set to a cylindrical structure. After the two are assembled, the displacement between the rear light lens barrel 310 and the main body lens barrel 100 in the radial plane is limited by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the outer circle of the rear light lens barrel 310 and the accuracy of the inner circle of the main body lens barrel 100 to achieve the centering of the lens barrel. Combined with the processing and assembly accuracy, the precise installation between the optical device and the lens barrel can be guaranteed, which can simplify the structure and significantly reduce the debugging steps and debugging costs. It can be understood that in this solution, the cross-sections of the main channel 110 and the insertion portion of the rear light lens barrel 310 are both circular. Compared with other cavities and insertion portions with non-circular cross-sections (such as polygonal cavities including rectangles, or special-shaped cavities with flat and curved inner walls), higher processing accuracy can be ensured through relatively simple processes. For example, a high-precision circular channel or circular outer contour can be obtained by rotating the blank with a tool.

[0130] In another embodiment, the cross-section of the first rear light channel 311 is circular, and the main lens barrel 100 is partially located in the first rear light channel 311. In other words, this embodiment is a situation in which the main lens barrel 100 is plugged into the rear light channel 310. For example, the front end of the main lens barrel 100 is plugged into the rear end of the first rear light channel 311. In order to adapt to the plug-in fit, the outer contour of the plug-in portion of the main lens barrel 100 is set to a circular shape and is compatible with the shape and size of the first rear light channel 311. For example, the main lens barrel 100 is entirely set to a cylindrical structure. Similarly, this embodiment can also serve the purpose of simplifying the structure, reducing the debugging steps and debugging costs. It should be noted that the cross-sectional shape of the main channel 110 in this embodiment is not limited to a circular shape.

[0131] It should be noted that the rear light assembly 300 usually also includes a single rear light lens barrel 310, and the main lens barrel 100 is nested and connected with the single rear light lens barrel 310. In this way, at least part of the components of the rear light optical module can be pre-assembled separately with the rear light lens barrel 310, and then the rear light lens barrel 310 can be nested and connected with the main lens barrel 100 for easy assembly.

[0132] Based on the aforementioned nested connection between the rear-light lens barrel 310 and the main lens barrel 100, referring to Figures 4 and 5, the rear-light optical module in some embodiments includes a first condenser lens 320 and a first aperture 330. The first condenser lens 320 is used to converge light, thereby reducing the light spot incident on the light receiving device, thereby reducing the target surface area of ​​the light receiving device, and ultimately reducing the size and cost of the light receiving device. At least one of the incident surface and the exit surface of the first condenser lens 320 is an aspherical surface. In this embodiment, the first condenser lens 320 is an aspherical lens to better converge light. The first aperture 330 is used to block stray light from the light receiving device, thereby improving the quality of the optical signal collected by the light receiving device and facilitating an increase in the optical signal-to-noise ratio. The light receiving device can be a photodetector that receives light emitted from the flow chamber assembly 400 and passing through the first condenser lens 320 and the first aperture 330.

[0133] In this embodiment, the light receiving component is connected to the rear light barrel 310, and the first aperture 330 is arranged between the main lens barrel 100 and the rear light barrel 310. It should be noted that only the position of the first aperture 330 relative to the main lens barrel 100 and the rear light barrel 310 is described here, and the connection relationship is not specifically limited. The first aperture 330 can be fixed to the main lens barrel 100, or to the rear light barrel 310, or can be fixed by the main lens barrel 100 and the rear light barrel 310 in a clamping manner, for example.

[0134] In some specific embodiments, the first aperture 330 is clamped between the main lens barrel 100 and the rear light lens barrel 310, further increasing the integrated installation function of the main lens barrel 100. In addition, compared with the method of fixing with adhesives or threaded fasteners, the clamping method can be more convenient for disassembly and assembly.

[0135] Based on the first aperture 330 being clamped between the main lens barrel 100 and the rear light lens barrel 310, in some embodiments, the rear light lens barrel 310 is plugged into the main channel 110 to compress the first aperture 330. Referring to Figures 4 and 5, the main channel 110 includes a first channel section 111 and a second channel section 112. The first channel section 111 and the second channel section 112 are arranged in sequence along the axial direction of the main channel 110, that is, the first channel section 111 is located to the left of the second channel section 112 in the figure. The diameter of the first channel section 111 is smaller than that of the second channel section 112, forming an abutment surface 113 at the intersection of the two. The front end of the rear light lens barrel 310 is plugged into the second channel section 112 to compress the first aperture 330 against the abutment surface 113. In this way, the rear light lens barrel 310 and the main lens barrel 100 are connected by plugging, which can simplify the assembly structure between the rear light lens barrel 310 and the main lens barrel 100 and facilitate assembly.

[0136] It should be noted that, in addition to the first channel section 111 and the second channel section 112 , the main channel 110 may further include other channel sections, such as a third channel section disposed on the left side of the first channel section 111 for inserting the front light barrel 210 .

[0137] In some specific embodiments, referring to Figures 2, 3, and 5, the wall of the second channel section 112 is provided with a first hole 120 extending through the outer surface of the main lens barrel 100. The first hole 120 is used to allow the first aperture 330 to move relative to the main lens barrel 100 in a through-direction, which is the aforementioned first direction. The first hole 120 is provided on one or two opposing sides of the wall of the second channel section 112, and the movement direction of the first aperture 330 is specifically a front-to-back direction. Movement in the aforementioned direction allows the first aperture 330 to be moved in and out of the main lens barrel 100, thereby enabling assembly of the first aperture 330. Furthermore, the position of the first aperture 330 can be fine-tuned after it is positioned between the main lens barrel 100 and the rear light barrel 310, thereby adjusting the position between the first aperture 330 and the first focusing lens 320. It should be noted that since the first aperture 330 can be moved laterally relative to the main lens barrel 100, when assembling or adjusting the first aperture 330, the rear light lens barrel 310 does not need to be completely withdrawn from the main channel 110, but only needs to be moved a short distance backward relative to the main lens barrel 100, which is convenient for operation.

[0138] Based on the first aperture 330 being clamped between the main lens barrel 100 and the rear light lens barrel 310, in some embodiments, the rear light lens barrel 310 is detachably connected to the main lens barrel 100, and when the rear light lens barrel 310 is in a non-compressed state, the first aperture 330 is configured to be movable relative to the main lens barrel 100 in a first direction, thereby enabling installation or adjustment of the first aperture 330. The rear light lens barrel 310 of this embodiment can be detachably connected to the main lens barrel 100 using the aforementioned plug-in fitting method, or can be detachably connected to the main lens barrel 100 using threaded fasteners or the like.

[0139] It should be noted that the “non-pressed state” referred to here includes both a state in which the rear light barrel 310 is completely separated from the main channel 110 and a state in which there is a gap between the rear light barrel 310 and the abutting surface 113 to allow the first aperture 330 to move.

[0140] To position the first aperture 330 during movement, referring to Figures 4 and 6 , in some embodiments, one of the main barrel 100 and the first aperture 330 is provided with a first positioning portion 130, and the other is provided with a second positioning portion 331. The first aperture 330 can be moved relative to the main barrel 100 in a first direction (e.g., left-right in Figure 6 ) via the first positioning portion 130 and the second positioning portion 331. In some specific embodiments, the first positioning portion 130 includes a positioning protrusion extending parallel to the axial direction of the main channel 110, such as a positioning post provided on the abutment surface 113. The second positioning portion 331 includes a positioning recess extending in the first direction, such as a strip-shaped positioning groove provided on the first aperture 330. The positioning protrusion is inserted into the positioning recess and can slide along the extending direction of the positioning recess for positioning. It is understood that the main barrel 100 can also be provided with a positioning groove, while the first aperture 330 is provided with a positioning post. Based on the aforementioned structure, the first aperture 330 of this embodiment only needs to move in the left-right direction, further reducing the debugging process.

[0141] It should be noted that when the abutment surface 113 is provided with a positioning post, a vertical reference plane parallel to the axial direction of the main channel 110 is established, and the projection of the first hole 120 in the reference plane should cover and be wider than the projection of the positioning post in the reference plane. In this way, the first aperture 330 can first be offset from the positioning post along the axial direction, enter the main channel 110 along the first direction, and then move axially toward the abutment surface 113 to allow the positioning post to be inserted into the positioning groove.

[0142] In other embodiments, one of the rear light barrel 310 and the first aperture 330 may be provided with a first positioning portion 130, and the other may be provided with a second positioning portion 331, and the first aperture 330 may be able to move relative to the main barrel 100 along the first direction through the first positioning portion 130 and the second positioning portion 331.

[0143] The above-mentioned embodiment describes a scheme for pressing the first aperture 330 by the rear light barrel 310 and the main body barrel 100. In some alternative embodiments, the first aperture 330 can also be pressed by a separate fixing member. The wall of the main body barrel 100 is provided with a first hole 120 that passes through the outer surface of the main body barrel 100. For example, the first hole 120 is provided on the wall of the second channel section 112. At least one end of the first aperture 330 extends from the first hole 120. The particle detection device also includes a fixing member, which is connected to the main body barrel 100 and acts on the protruding part of the first aperture 330 to fix the first aperture 330 to the main body barrel 100, or the fixing member is connected to the rear light barrel 310 and acts on the protruding part of the first aperture 330 to fix the first aperture 330 to the rear light barrel 310.

[0144] The fixing member can be a threaded fastener. For example, a through hole is provided on the first aperture 330, and an axial threaded hole is provided on the outer side of the main lens barrel 100. The threaded fastener passes through the through hole and is screwed into the threaded hole to fix the first aperture 330 to the main lens barrel 100. It can be understood that the walls of the main lens barrel 100 on opposite sides are provided with first holes 120, and the two ends of the first aperture 330 extend from the corresponding first holes 120 and are respectively fixed by fixing members, so as to achieve a stable connection of the first aperture 330.

[0145] Based on the first aperture being positioned between the main lens barrel 100 and the rear light lens barrel 310, referring to FIG5 , in some embodiments, a first condenser lens 320 is connected to the main lens barrel 100 and positioned between the flow chamber assembly 400 and the first aperture 330. In this manner, the illumination light emitted from the flow chamber assembly 400 is first converged by the first condenser lens 320 before passing through the first aperture 330, thereby reducing the area of ​​the opening in the first aperture 330. Specifically, in the illustrated embodiment, the first condenser lens 320 is mounted in the first channel section 111.

[0146] In other embodiments, the first condensing lens 320 is connected to the rear light barrel 310 and is located between the first aperture 330 and the light receiving device, thereby improving the integration of the rear light assembly 300 .

[0147] The above embodiment describes a scheme in which some components of the rear light optical module are connected to the rear light lens barrel 310. In some embodiments, all optical components of the rear light optical module can also be connected to the rear light lens barrel 310. Specifically, the rear light optical module includes the aforementioned first focusing lens 320 and first aperture 330, and the first focusing lens 320 and the first aperture 330 are both connected to the rear light lens barrel 310. In addition, the light receiving component is also connected to the rear light lens barrel 310. In this way, the rear light assembly 300 can be pre-assembled and then connected to the main lens barrel 100 to further improve the integration of the rear light assembly 300.

[0148] It should be noted that the aforementioned scheme based on the nested connection between the front light lens barrel 210 and the main body lens barrel 100, and the scheme based on the nested connection between the rear light lens barrel 310 and the main body lens barrel 100 can be combined with each other. For example, when the front light lens barrel 210 and the main body lens barrel 100 are nested and connected as the basis, the rear light assembly 300 includes the rear light lens barrel 310 and the rear light optical module, the rear light optical module is at least partially connected to the rear light lens barrel 310, and the rear light lens barrel 310 is nested and connected to the main body lens barrel 100. The specific connection scheme can be understood with reference to the second embodiment and the corresponding specific embodiments. In addition, the rear light lens barrel 310 can also serve as the pressing member of the first embodiment to press the first aperture 330. The specific pressing scheme can also be understood with reference to the second embodiment and the corresponding specific embodiments. Similarly, when the backlight lens barrel 310 is nested with the main body lens barrel 100, the front light assembly 200 may include the front light lens barrel 210 and the front light optical module, the front light optical module is connected to the front light lens barrel 210, and the front light lens barrel 210 is nested with the main body lens barrel 100. The specific connection scheme can be understood with reference to the first embodiment and the corresponding specific embodiments. It can be understood that when the front light lens barrel 210 and the backlight lens barrel 310 are both nested with the main body lens barrel 100, the front light assembly 200 and the backlight assembly 300 will be installed with the main body lens barrel 100 as a reference. It is only necessary to ensure the processing accuracy of the main body lens barrel 100 and the assembly accuracy with the front light lens barrel 210 and the backlight lens barrel 310 respectively, so that the alignment of the front light lens barrel 210 and the backlight lens barrel 310 can be guaranteed. Compared with the method of adjusting each module separately, the adjustment steps and costs can be significantly reduced, and the assembly operation can also be simplified.

[0149] The following description will be based on the connection between the flow chamber assembly 400 and the main body barrel 100. Referring to Figures 2 to 5, the particle detection device in the third embodiment includes the main body barrel 100, the front light assembly 200, the rear light assembly 300, the flow chamber assembly 400, the light source 700, and the light receiving assembly. The flow chamber assembly 400 includes a liquid injection portion 410, a detection portion 420, and a liquid discharge portion 430 arranged in sequence along the flow direction of the sample liquid. The liquid injection portion 410 is used to allow the sample liquid to be tested to flow into the detection portion 420. The light emitted by the front light assembly 200 illuminates the sample liquid to be tested from the side of the detection portion 420. The illuminated sample liquid to be tested is discharged from the liquid discharge portion 430 into the drainage pipe.

[0150] Specifically, referring to FIG8 , a second through-hole 140 is provided in the wall of the main barrel 100 , through which the detection unit 420 can enter the main channel 110 . The second through-hole 140 can be configured as a strip-shaped hole extending in a first direction to accommodate movement of the flow chamber assembly 400 in the first direction. Furthermore, a portion of the liquid injection portion 410 can also be provided through the second through-hole 140 .

[0151] The front light assembly 200 includes a front light barrel 210 and a front light optical module. The front light barrel 210 has a front light channel 211. The front light optical module is disposed within the front light channel 211. The irradiated light passes through the front light optical module, which shapes the irradiated light. The shaped light then passes from the incident side to the detection unit 420. The back light assembly 300 includes a back light barrel 310 and a back light optical module. The back light barrel 310 has at least a first back light channel 311. The back light optical module is at least partially disposed within the first back light channel 311. The back light optical module has at least a first optical axis. In other words, the back light optical module in some embodiments may also have a second optical axis, which will be described in subsequent embodiments. The irradiated light emitted from the exit side of the detection unit 420 passes through the back light optical module, which shapes the irradiated light emitted from the exit side of the detection unit 420. In this embodiment, the front light barrel 210 is nested and connected with the main body barrel 100, and the rear light barrel 310 is nested and connected with the main body barrel 100, and the central axis of the main body channel 110, the optical axis of the front light optical module, the first optical axis of the rear light optical module, the central axis of the front light barrel 210, and the central axis of the rear light barrel 310 are approximately coaxial. In some embodiments, the front light optical module includes a plurality of lenses and other components, and the rear light assembly 300 includes a rear light optical module, which includes a first condenser lens 320, a first aperture 330, and other components.

[0152] In this embodiment, at least the detection portion 420 of the flow chamber assembly 400 is located within the main channel 110, so that the illumination light emitted by the front light assembly 200 can illuminate the detection portion 420 within the main channel 110, and the illumination light emitted after irradiating the sample liquid to be tested can be shaped by the rear light optical module. Along the flow direction of the sample liquid to be tested, the central axis of the main channel 110 (which can also be said to be the optical axis of the front light optical module, the first optical axis of the rear light optical module, the central axis of the front light barrel 210 or the central axis of the rear light barrel 310) is located at both ends of the detection part 420. That is, when the flow chamber assembly 400, the main barrel 100, the front light assembly 200 and the rear light assembly 300 are assembled, along the flow direction of the sample liquid to be tested, the positions of the detection part 420, the front light assembly 200 and the rear light assembly 300 correspond to each other, ensuring that the irradiation light emitted by the front light assembly 200 can irradiate the sample liquid to be tested in the detection part 420, and the irradiation light emitted by the detection part 420 can also accurately pass through the rear light optical module, thereby eliminating the adjustment link of the flow chamber assembly 400 in the flow direction of the sample liquid to be tested, making the assembly and debugging steps simpler.

[0153] In addition, in this embodiment, the main lens barrel 100 serves as a medium to connect the flow chamber assembly 400, the front light assembly 200 and the rear light assembly 300. The flow chamber assembly 400, the front light assembly 200 and the rear light assembly 300 all use the main lens barrel 100 as an installation reference. The flow chamber assembly 400, the front light assembly 200 and the rear light assembly 300 can be accurately positioned through the main lens barrel 100, which is conducive to reducing the debugging links and reducing the debugging costs.

[0154] Based on the third embodiment, in some embodiments, one of the main barrel 100 and the flow chamber assembly 400 is provided with a third positioning portion, and the other is provided with a fourth positioning portion. The flow chamber assembly 400 can be positioned relative to the main barrel 100 by the third and fourth positioning portions. The flow chamber assembly 400 is configured to be movable relative to the main barrel 100 in a first direction, thereby achieving alignment adjustment between the light emitted by the front light assembly 200 and the sample liquid to be tested. Specifically, the flow channel within the detection section 420 is arranged in a vertical direction, and particles in the sample liquid to be tested queue through the flow channel and are sequentially illuminated. After the height of the detection section 420 is determined by the third and fourth positioning portions, it is only necessary to further define the position between the detection section 420 and the light along the first direction (e.g., the left-right direction in FIG. 6 ) so that the light emitted by the front light assembly 200 can accurately illuminate the particles in the sample liquid to be tested. In some embodiments, the flow chamber assembly 400 and the main barrel 100 are further provided with a first-direction position-limiting structure. Once the flow chamber assembly 400 is mounted to the main barrel 100, its position in the first direction is already fixed and requires no adjustment. In this embodiment, however, the flow chamber assembly 400 can be moved in the first direction to adjust its position via the third and fourth positioning portions. Due to the extremely small diameter of the particles, this embodiment significantly reduces the installation precision requirements for the flow chamber assembly 400 and the main barrel 100 in the first direction, thereby reducing processing costs.

[0155] On the basis of the main body barrel 100 and the flow chamber assembly 400 being positioned by the third positioning portion and the fourth positioning portion, referring to Figures 7 and 8, in some embodiments, the liquid injection portion 410 is provided with a third positioning portion, and the main body barrel 100 is provided with a fourth positioning portion. The third positioning portion includes a third positioning surface 412 of the liquid injection portion 410, and the fourth positioning portion includes a fourth positioning surface 160 of the main body barrel 100. The third positioning surface 412 is in contact with the fourth positioning surface 160, which can limit the relative movement between the flow chamber assembly 400 and the main body barrel 100 along the flow direction of the sample liquid to be tested. At the same time, the flow chamber assembly 400 and the main body barrel 100 can slide along the first direction in a plane perpendicular to the flow direction of the sample liquid to be tested.

[0156] In other embodiments, the third positioning portion and the fourth positioning portion can also be used for axial positioning of the flow chamber assembly 400. Referring to Figures 7 and 8, the third positioning portion includes a first positioning surface 411 of the liquid injection portion 410 perpendicular to the axial direction of the main channel 110, and the fourth positioning portion includes a second positioning surface 150 of the main barrel 100 perpendicular to the axial direction of the main channel. The first positioning surface 411 is in contact with the second positioning surface 150, which can limit the relative movement between the flow chamber assembly 400 and the main barrel 100 along the axial direction of the main channel 110.

[0157] Based on the positioning of the main barrel 100 and the flow chamber assembly 400 by the third positioning portion and the fourth positioning portion, in some specific embodiments, the third positioning portion includes the aforementioned first positioning surface 411 and third positioning surface 412, and the fourth positioning portion includes the aforementioned second positioning surface 150 and fourth positioning surface 160, so that the displacement of the flow chamber assembly 400 along the flow direction and axial direction of the sample liquid to be tested is restricted.

[0158] The flow chamber assembly 400 is typically placed vertically, with the sample liquid to be tested flowing from bottom to top. Therefore, the liquid injection portion 410 is connected to the bottom side of the main body barrel 100. To support and limit the flow chamber assembly 400, the particle detection device further includes a support member 600 connected to the main body barrel 100. The support member 600 abuts the liquid injection portion 410 against the fourth positioning surface 160 along the flow direction of the sample liquid to be tested, and abuts the liquid injection portion 410 against the second positioning surface 150 along the axial direction of the main body channel 110. Specifically, in the embodiment shown in Figures 7 and 8, the support member 600 is configured as a pressing structure, one end of which is connected to the bottom side of the main body barrel 100, such as the fourth positioning surface 160, and the other end is configured as an elastic end. The elastic end abuts against the liquid injection portion 410, cooperating with the fourth positioning surface 160 to fully limit the vertical direction and the axial direction, respectively. The second mounting portion 414 is further formed with a flange 416, and the elastic end abuts against the flange 416. In this embodiment, by providing elastic force to the support member 600, when the support member 600 elastically abuts against the liquid injection portion 410, the flow chamber assembly 400 can move relative to the main barrel 100 in the first direction. In this way, the operator does not need to support the flow chamber assembly 400 during the adjustment process, which facilitates operation.

[0159] When the fourth positioning portion includes the aforementioned second positioning surface 150 and fourth positioning surface 160, referring to Figures 7 and 8, in some embodiments, the second positioning surface 150 and fourth positioning surface 160 on the main lens barrel 100 are formed on the outer side of the lens barrel. For example, a mounting groove can be formed on the outer side wall of the main lens barrel 100. Different wall surfaces of the mounting groove respectively form the second positioning surface 150 and the fourth positioning surface 160. For example, when the main lens barrel 100 has the second positioning surface 150, the groove wall surface of the mounting groove perpendicular to the axial direction of the main lens barrel 100 is set as the second positioning surface. When the main lens barrel 100 has the fourth positioning surface 160, the groove wall surface of the mounting groove perpendicular to the flow direction of the sample liquid to be tested is set as the fourth positioning surface 160. The mounting groove can be formed by removing material to facilitate processing.

[0160] The liquid injection portion 410 can be formed using a process such as injection molding and specifically includes a first mounting portion 413 and a second mounting portion 414. The first mounting portion 413 is perpendicular to the axial direction of the main channel 110 (e.g., arranged vertically). A side surface (e.g., the rear surface) of the first mounting portion 413 is configured as a first positioning surface 411, and its upper surface serves as the aforementioned third positioning surface 412. The second mounting portion 414 is connected to a side of the first mounting portion 413 and is perpendicular to the flow direction of the sample liquid to be tested (e.g., arranged horizontally). A side surface (e.g., the upper surface) of the second mounting portion 414 serves as the aforementioned third positioning surface 412. The detection portion 420 is connected to the second mounting portion 414.

[0161] The main lens barrel 100 of this embodiment is provided with the aforementioned mounting groove, and different groove wall surfaces of the mounting groove are respectively set as the second positioning surface 150 and the fourth positioning surface 160, the first mounting portion 413 and the second mounting portion 414 are at least partially located in the mounting groove, and the first positioning surface 411 is in contact with the second positioning surface 150, and the third positioning surface 412 is in contact with the fourth positioning surface 160.

[0162] In order to fix the flow chamber assembly 400, the particle detection device in some embodiments also includes a locking member, which is connected to the main barrel 100 and the flow chamber assembly 400, and has a first state for restricting the movement of the flow chamber assembly 400, and a second state for unlocking. When in the second state, the flow chamber assembly 400 can move in a first direction relative to the main barrel 100.

[0163] The locking member can be a threaded fastener, the liquid injection part 410 is provided with a connecting hole 415, and the main barrel 100 is provided with a threaded hole 151. When the threaded fastener is in a first state, it is passed through the connecting hole 415 and screwed into the threaded hole 151. When the threaded fastener is in a second state, it is separated from the threaded hole 151 so that the flow chamber assembly 400 can move relative to the main barrel 100 along the first direction. Specifically, in the embodiments shown in Figures 7 and 8, the first mounting part 413 is provided with the aforementioned connecting hole 415, and the connecting hole 415 passes through to the first positioning surface 411 and extends along the first direction. The second positioning surface 150 is provided with a threaded hole 151.

[0164] In some alternative embodiments, the second hole 140 can also be used for positioning in the first direction. For example, the wall of the main channel 110 is provided with a second hole 140 that extends through the outer surface of the main barrel 100. The second hole 140 extends along the first direction and is, for example, provided as a strip-shaped hole. The liquid injection portion 410 is provided through the second hole 140 and is capable of moving within the second hole 140 along the first direction. In this embodiment, the liquid injection portion 410 is provided with a third positioning portion, and the main barrel 100 is provided with a fourth positioning portion. The fourth positioning portion includes the hole wall of the second hole 140 extending along the first direction, and the third positioning portion includes the side of the hole wall against which the liquid injection portion 410 abuts.

[0165] Specifically, the liquid injection portion 410 of this embodiment includes the aforementioned second mounting portion 414 and boss 417. The second mounting portion 414 and boss 417 are arranged along the flow direction of the sample liquid to be tested. For example, the boss 417 is connected to the upper side of the second mounting portion 414. The end of the boss 417 away from the second mounting portion 414 is connected to the detection portion 420. The second mounting portion 414 and boss 417 can be formed into an integral structure through a process such as injection molding.

[0166] In this embodiment, the boss 417 is provided through the second hole 140, and the third positioning portion includes the boss 417 abutting against the side surface of the hole wall of the second hole 140. The boss 417 can be a circular boss as shown in the figure, or a rectangular boss.

[0167] Based on the third embodiment, in some embodiments, the optical axis of the front light assembly 200 is parallel to the central axis of the main body channel 110, and the distance between the optical axis and the central axis is within a preset range. It should be noted that the optical axis and the central axis are parallel, and the distance between them is within a preset range, including a distance of zero, that is, the optical axis can coincide with the central axis or deviate from the central axis.

[0168] In some specific embodiments, the distance between the optical axis and the central axis is less than or equal to 0.02 mm.

[0169] On the basis of the third embodiment, in some embodiments, the optical axis of the front light assembly 200 intersects with the central axis of the main body channel 110, and the angle between the optical axis and the central axis is within a preset range.

[0170] In some specific embodiments, the angle between the optical axis and the central axis is less than or equal to 5 mrad.

[0171] It should be noted that the solution based on the connection between the flow chamber assembly 400 and the main body barrel 100 can be combined with the aforementioned solution based on the nested connection between the front light barrel 210 and the main body barrel 100, and the solution based on the nested connection between the rear light barrel 310 and the main body barrel 100. For example, the front light assembly 200 includes the front light barrel 210, the front light optical module is connected to the front light barrel 210, and the front light barrel 210 is further nested and connected with the main body barrel 100. The specific nesting solution can be understood with reference to the first embodiment and the corresponding specific embodiments. For another example, the rear light assembly 300 includes the rear light barrel 310, the rear light optical module is at least partially connected to the rear light barrel 310, and the rear light barrel 310 is further nested and connected with the main body barrel 100. The specific nesting solution can be understood with reference to the second embodiment and the corresponding specific embodiments.

[0172] The following will describe a typical combination of the present invention in conjunction with Figures 2 to 8. The particle detection device includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700 and a light receiving assembly. The main lens barrel 100 has a main channel 110 with a circular cross-section. The front light assembly 200 has a front light lens barrel 210 and a front light optical module. The front light optical module includes multiple lenses. The light source 700 and the multiple lenses are all connected to the front light lens barrel 210, and the multiple lenses are located in the front light channel 211 of the front light lens barrel 210. The rear end of the front light lens barrel 210 is plugged into the front end of the main channel 110. The backlight assembly 300 comprises a backlight barrel 310 and a backlight optical module. The front end of the backlight barrel 310 is plugged into the rear end of the main body channel 110. The backlight optical module includes a first condenser lens 320 and a first aperture 330. The first aperture 330 is clamped between the backlight barrel 310 and the main body channel 100. The first condenser lens 320 is connected to the main body channel 110 and is located between the flow chamber assembly 400 and the first aperture 330. The light receiving assembly is connected to the backlight barrel 310. The flow chamber assembly 400 is positioned and connected to the main body channel 100 via third and fourth positioning portions and secured by a locking member. The detection unit 420 of the flow chamber assembly 400 is located within the main body channel 110.

[0173] The above structure has the following advantages:

[0174] 1. The front light lens barrel 210 and the rear light lens barrel 310 are connected to the main lens barrel 100 in a nested manner. When assembling, just insert the front light lens barrel 210 and the rear light lens barrel 310 into the main lens barrel 100, which is convenient for assembly.

[0175] 2. The cross-section of the main channel 110, the rear end of the front light lens barrel 210, and the front end of the rear light lens barrel 310 are all circular, which are easy to process and can ensure high processing accuracy. After the front light lens barrel 210 and the rear light lens barrel 310 are inserted into the main lens barrel 100, the position between the front light assembly 200 and the rear light assembly 300 is guaranteed by relying on their respective processing accuracy, which can significantly reduce the number of sliding mechanisms and debugging dimensions, and reduce material and debugging costs.

[0176] 3. The front light assembly 200, the rear light assembly 300 and the flow chamber assembly 400 are all installed based on the main barrel 100, which helps to reduce dimensional transmission errors.

[0177] 4. The first aperture 330 is fixed by clamping. Once the rear light barrel 310 is plugged into place, the first aperture 330 can be fixed. This facilitates assembly, and the rear light barrel 310 will not hinder the movement of the first aperture 330 when it is not compressed.

[0178] A fourth embodiment of the present invention further provides a particle detection device. The particle detection device in the fourth embodiment includes a main lens barrel 100, a front light assembly 200, a rear light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The flow chamber assembly 400 includes an injection portion 410, a detection portion 420, and a discharge portion 430 arranged in sequence along the flow direction of the sample liquid. The injection portion 410 is used to allow the sample liquid to be tested to flow into the detection portion 420. The light emitted by the front light assembly 200 illuminates the sample liquid to be tested from the side of the detection portion 420. The illuminated sample liquid to be tested is discharged from the discharge portion 430 into the drainage pipe.

[0179] The front light assembly 200 includes a front light optical module, which shapes the irradiated light, and the shaped light enters the detection unit 420 from the incident side. The rear light assembly 300 includes a rear light optical module, which has at least a first optical axis. The irradiated light emitted from the exit side of the detection unit 420 passes through the rear light optical module, and the rear light optical module shapes the irradiated light emitted from the exit side of the detection unit 420. In this embodiment, the central axis of the main channel 110, the optical axis of the front light optical module, and the first optical axis of the rear light optical module are approximately coaxial. In some embodiments, the front light optical module includes a plurality of lenses and other devices, and the rear light assembly 300 includes a rear light optical module, and the rear light optical module includes a first focusing lens 320, a first aperture 330 and other devices.

[0180] The difference between this embodiment and the third embodiment is that: in the third embodiment, the front light optical module is connected to the main lens barrel 100 through the front light lens barrel 210, and the rear light optical module is connected to the main lens barrel 100 through the rear light lens barrel 310. This embodiment is not limited to this, and the front light optical module and the rear light optical module can be connected to the main lens barrel 100 in any direct or indirect manner.

[0181] Based on the fourth embodiment, in some embodiments, one of the main barrel 100 and the flow chamber assembly 400 is provided with a third positioning portion, and the other is provided with a fourth positioning portion. The flow chamber assembly 400 can be positioned relative to the main barrel 100 by the third and fourth positioning portions. The flow chamber assembly 400 is configured to be movable relative to the main barrel 100 in a first direction, thereby achieving alignment adjustment between the light emitted by the front light assembly 200 and the sample liquid to be tested. Specifically, the flow channel within the detection section 420 is arranged in a vertical direction, and particles in the sample liquid to be tested queue through the flow channel and are sequentially illuminated. After the height of the detection section 420 is determined by the third and fourth positioning portions, it is only necessary to further define the position between the detection section 420 and the light along the first direction (e.g., the left-right direction in FIG. 6 ) so that the light emitted by the front light assembly 200 can accurately illuminate the particles in the sample liquid to be tested. In some embodiments, the flow chamber assembly 400 and the main barrel 100 are further provided with a first-direction position-limiting structure. Once the flow chamber assembly 400 is mounted to the main barrel 100, its position in the first direction is already fixed and requires no adjustment. In this embodiment, however, the flow chamber assembly 400 can be moved in the first direction to adjust its position via the third and fourth positioning portions. Due to the extremely small diameter of the particles, this embodiment significantly reduces the installation precision requirements for the flow chamber assembly 400 and the main barrel 100 in the first direction, thereby reducing processing costs.

[0182] On the basis of the main body barrel 100 and the flow chamber assembly 400 being positioned by the third positioning portion and the fourth positioning portion, referring to Figures 7 and 8, in some embodiments, the liquid injection portion 410 is provided with a third positioning portion, and the main body barrel 100 is provided with a fourth positioning portion. The third positioning portion includes a third positioning surface 412 of the liquid injection portion 410, and the fourth positioning portion includes a fourth positioning surface 160 of the main body barrel 100. The third positioning surface 412 is in contact with the fourth positioning surface 160, which can limit the relative movement between the flow chamber assembly 400 and the main body barrel 100 along the flow direction of the sample liquid to be tested. At the same time, the flow chamber assembly 400 and the main body barrel 100 can slide along the first direction in a plane perpendicular to the flow direction of the sample liquid to be tested.

[0183] In other embodiments, the third positioning portion and the fourth positioning portion can also be used for axial positioning of the flow chamber assembly 400. Referring to Figures 7 and 8, the third positioning portion includes a first positioning surface 411 of the liquid injection portion 410 perpendicular to the axial direction of the main channel 110, and the fourth positioning portion includes a second positioning surface 150 of the main barrel 100 perpendicular to the axial direction of the main channel. The first positioning surface 411 is in contact with the second positioning surface 150, which can limit the relative movement between the flow chamber assembly 400 and the main barrel 100 along the axial direction of the main channel 110.

[0184] The sample liquid to be tested passes through the flow chamber one by one under the action of fluid mechanics, forms a scattered signal under the irradiation of the laser, and emits scattered light to the entire space. The photoelectric detector receives the scattered light and obtains information about the sample liquid to be tested. Different scattered light collection angles correspond to different information about the sample liquid to be tested. In the related art, generally one detector is used to collect scattered light in three angle ranges: small-angle forward light, medium-angle forward light, and large-angle forward light at the same time, and the scattered light is not converged. The angle aperture constraint and detector reception are directly used, which leads to the following deficiencies in the specific application of the blood sample analyzer: the optical signal quality collected by the detector is poor, and a detector with a large target surface is required to receive the scattered light. Therefore, the detectors provided by the related art generally have the problems of poor detection signal, large size, and high cost. To solve the aforementioned problems, based on the first to fourth embodiments, some embodiments of the present invention further propose a rear light assembly 300. Referring to Figures 3, 5, and 9, the rear light optical module includes a first focusing lens 320, a first aperture 330, a reflector 340, a first light receiving device 810, and a second light receiving device 820, wherein the first focusing lens 320, the first aperture 330, and the reflector 340 are arranged in sequence along the light emitting direction of the front light assembly 200, and the first light receiving device 810 and the second light receiving device 820 are arranged on the rear side of the reflector 340 along the light emitting direction.

[0185] The first condenser lens 320 is configured to converge at least first illumination light L1 generated by the frontlight assembly 200 and irradiated onto the sample liquid to be tested within a first angular range, and second illumination light L2 generated by the frontlight assembly 200 and irradiated onto the sample liquid to be tested within a second angular range, such as small-angle forward light and large-angle forward light. The first illumination light L1 within the first angular range specifically refers to scattered light generated by the frontlight assembly 200 and irradiated onto the sample liquid to be tested, and having an angle within the first angular range with respect to the optical axis of the light emitted by the frontlight assembly 200. The second illumination light L2 within the second angular range specifically refers to scattered light generated by the frontlight assembly 200 and irradiated onto the sample liquid to be tested, and having an angle within the second angular range with respect to the optical axis of the light emitted by the frontlight assembly 200.

[0186] The reflector 340 is used to reflect the light formed by the second illumination light L2 after being converged by the first condenser lens, thereby separating the first illumination light L1 from the second illumination light L2. The first light receiving device 810 is used to at least receive the light converged by the first illumination light L1 through the first condenser lens 320, and the second light receiving device 820 is used to receive the light converged by the second illumination light L2 through the first condenser lens 320 and formed by the reflector 340. Specifically, the light converged by the first condenser lens 320 can pass from the side of the reflector 340, or a light-transmitting portion can be provided on the reflector 340 so that the light converged by the first condenser lens 320 passes through the light-transmitting portion of the reflector 340. In this embodiment, the second illumination light L2 is separated by the reflector 340, and the first light receiving device 810 and the second light receiving device 820 respectively receive light with different scattering angles. This improves the quality of the optical signal received by each light receiving device and reduces the target surface area, volume, and cost of each light receiving device.

[0187] In some embodiments, the first condenser lens 320 is further configured to converge third illumination light L3 generated by the frontlight assembly 200 and irradiated onto the sample liquid to be tested, and within a third angular range. The third illumination light L3 within the third angular range specifically refers to scattered light generated by the frontlight assembly 200 and irradiated onto the sample liquid to be tested, and having an angle within the third angular range with the optical axis of the light emitted by the frontlight assembly 200.

[0188] Accordingly, the first light receiving device 810 is further configured to receive light formed by the third illumination light L3 converged by the first condenser lens 320, thereby separating the first illumination light L1 from the second illumination light L2 and the third illumination light L3. The third illumination light L3 converged by the first condenser lens 320 can pass laterally through the reflector 340, or a light-transmitting portion can be provided on the reflector 340 so that the light of the third illumination light L3 converged by the first condenser lens 320 passes through the light-transmitting portion of the reflector 340.

[0189] In some embodiments, each angle in the second angle range is smaller than each angle in the first angle range, and each angle in the third angle range is larger than each angle in the second angle range and smaller than each angle in the first angle range. Specifically, the first illumination light L1 is scattered light at a large angle, the second illumination light L2 is scattered light at a small angle, and the third illumination light L3 is scattered light at a medium angle.

[0190] In some specific embodiments, each angle in the first angle range is greater than 20°, each angle in the third angle range is greater than or equal to 10°, and each angle in the second angle range is greater than 0°. Further, each angle in the first angle range is greater than 20° and less than or equal to 45°, each angle in the third angle range is greater than or equal to 10° and less than or equal to 20°, and each angle in the second angle range is greater than 0° and less than 10°. Still further, each angle in the first angle range is greater than 20° and less than or equal to 70°, each angle in the third angle range is greater than or equal to 10° and less than or equal to 20°, and each angle in the second angle range is greater than 0° and less than 10°. Since the angles in the second angle range are relatively close to the angles in the third angle range, separating the second illumination light L2 and receiving it through a separate light receiving device can reduce errors and improve recognition accuracy.

[0191] Collecting scattered light at different angles can reflect different information about the particles to be tested. Specifically, collecting the second illumination light L2 at 0° to 10° is mainly used to reflect the volume size of the particles. Since the third illumination light L3 at 10° to 20° is more sensitive to the refractive index of the cell membrane, cytoplasm, and nuclear membrane, collecting the third illumination light L3 at 10° to 20° is mainly used to reflect the particle size (complexity) information of the particle particulate matter. Collecting the first illumination light L1 at 20° to 70° is mainly used to increase the accuracy of particle identification. In a specific embodiment, the light receiving device is used to detect the white blood cell classification count value. In this embodiment, if only the second illumination light L2 and the third illumination light L3 are collected, when testing a sample with a large number of eosinophils, eosinophils and neutrophils are difficult to distinguish. If the first illumination light L1, the second illumination light L2, and the third illumination light L3 are collected at the same time, the scattered light collected in the three angle ranges can be used to increase the accuracy of cell identification, so that three-dimensional data of the particles can be obtained. By collecting scattered light over a wide angle range, more particle information can be obtained, thereby improving the accuracy of particle classification.

[0192] In some embodiments, the first light receiving device 810 and the second light receiving device 820 both have a light receiving area, and the light receiving areas of the first light receiving device 810 and the second light receiving device 820 are oriented in the same direction, that is, toward the first condensing lens 320 . Along the axial direction of the main channel 110, the distance between the first light receiving device 810 and the first focusing lens 320 is smaller than the distance between the second light receiving device 820 and the first focusing lens 320. In other words, the first light receiving device 810 is closer to the first focusing lens 320 than the second light receiving device 820. The reason for this arrangement is that due to the converging effect of the first focusing lens 320, the first illumination light L1 and the third illumination light L3 will eventually converge into a light spot. The first light receiving device 810 is set closer to the first focusing lens 320, and the first illumination light L1 and the third illumination light L3 are irradiated to the first light receiving device 810 before they converge together, thereby forming two separate light spots, which makes it easier for the first light receiving device 810 to receive the first illumination light L1 and the third illumination light L3 after they are converged by the first focusing lens 320. In this embodiment, corresponding to the first illumination light L1 and the third illumination light L3, the first light receiving device 810 can be provided with two parallel light receiving areas, that is, light in two angle ranges is received by one light receiving device, which can reduce the number of light receiving devices and help reduce costs.

[0193] In some embodiments, referring to FIG9 , the rear-light optical module further includes a third aperture 350, which is located between the reflector 340 and the second light receiving device 820 along a direction parallel to the axial direction of the first rear-light channel 311. The direction parallel to the axial direction of the first rear-light channel 311 means that the third aperture 350 is not directly aligned with the reflector 340. The third aperture 350 is used to block stray light from irradiating the second light receiving device 820, thereby further improving the quality of the optical signal collected by the second light receiving device 820 and facilitating an improvement in the optical signal-to-noise ratio.

[0194] In some embodiments, referring to Figure 9, the rear-light optical module also includes a fourth aperture 360, which is located in the first rear-light channel 311 and between the reflector 340 and the first light receiving device 810 along the axial direction of the first rear-light channel 311. The fourth aperture 360 ​​is used to block stray light that is irradiated to the first light receiving device 810, thereby further improving the quality of the optical signal collected by the first light receiving device 810, which is beneficial to improving the optical signal-to-noise ratio.

[0195] In some embodiments, referring to Figure 9, the rear light optical module also includes a third focusing lens 370, which is located in the first rear light channel 311 and is located between the reflector 340 and the first light receiving device 810 along the axial direction of the first rear light channel 311. The third focusing lens 370 can further converge the light irradiated to the first light receiving device 810, reduce the light spot irradiated to the first light receiving device 810, thereby reducing the target surface area of ​​the first light receiving device 810, and further reducing the volume and cost of the first light receiving device 810.

[0196] In some embodiments, the rear light barrel 310 of this embodiment adopts a dual-channel design, and the rear light barrel 310 also includes a second rear light channel 312. The first rear light channel 311 and the second rear light channel 312 are arranged in parallel. The first rear light channel 311 is used to at least allow light formed by the first illumination light L1 to pass through the first condenser lens 320, and the second rear light channel 312 is used to allow light formed by the second illumination light L2 to pass through the first condenser lens 320 and reflected by the reflector 340. Specifically, the first light receiving device 810 is disposed at the rear end of the first rear light channel 311, and the second light receiving device 820 is disposed at the rear end of the second rear light channel 312. In some embodiments, the first rear light channel 311 is also used to allow light formed by the third illumination light L3 to pass through the first condenser lens 320.

[0197] Some embodiments of the present invention further provide another backlight assembly 300, which differs from the aforementioned backlight assembly 300 in that: the aforementioned backlight assembly 300 is provided with a first light receiving device 810 and a second light receiving device 820. The first light receiving device 810 receives light formed by the first illumination light L1 converged by the first condenser lens 320 and the third illumination light L3 converged by the first condenser lens 320. The backlight assembly 300 in this embodiment is further provided with a third light receiving device, wherein the second light receiving device 820 is used to receive light formed by the second illumination light converged by the first condenser lens 320 and reflected by the reflector 340, and the third light receiving device is used to receive light formed by the third illumination light converged by the first condenser lens 320. In other words, light at different angles is received by different light receiving devices.

[0198] Similarly, in this embodiment, each angle in the second angle range is smaller than each angle in the first angle range, and each angle in the third angle range is larger than each angle in the second angle range and smaller than each angle in the first angle range. Specifically, the first illumination light L1 is scattered light at a large angle, the second illumination light L2 is scattered light at a small angle, and the third illumination light L3 is scattered light at a medium angle.

[0199] Based on the first to fourth embodiments, some embodiments of the present invention further propose a front light assembly 200, which includes a cylindrical lens 230 and a second focusing lens 240. The light source 700, the cylindrical lens 230 and the second focusing lens 240 are arranged in sequence along the axial direction of the front light channel 211, and at least the cylindrical lens 230 and the second focusing lens 240 are located in the front light channel 211.

[0200] Light source 700 is configured as a laser capable of generating laser light. Light source 700 is movable in a vertical plane perpendicular to the central axis of front light barrel 210, ensuring that the central axis of the light beam is approximately coaxial with the optical axis of the lens. Once light source 700 has been commissioned, it can be secured, for example, by applying glue. Cylindrical lens 230 converges the incident light beam in the direction of the sample liquid's flow, while second condenser lens 240 converges the incident light beam in both the direction of the sample liquid's flow and the first direction.

[0201] In some embodiments, referring to Figures 5 and 9, the front light optical module also includes an aspheric lens 250. The aspheric lens 250 is located in the front light channel 211 and between the light source 700 and the cylindrical lens 230. The aspheric lens 250 is used to collimate the light beam emitted by the light source 700.

[0202] In some embodiments, referring to Figures 5 and 9, the front light optical module further includes an optical isolator 260, which is located in the front light channel 211 and between the aspheric lens 250 and the cylindrical lens 230. The optical isolator 260 is used to prevent the incident light beam from being irradiated onto the cylindrical lens 230 and the second focusing lens 240 to form a reflected light beam, thereby improving the signal-to-noise ratio of the incident light beam irradiated onto the sample flow in the flow chamber.

[0203] In some embodiments, referring to FIG. 5 and FIG. 9 , the front light optical module further includes a second aperture 220 . The second aperture 220 is located in the front light channel 211 and between the light source 700 and the aspheric lens 250 .

[0204] In some embodiments, referring to Figure 5, along the axial direction of the front light barrel, the front light channel 211 includes a plurality of first mounting cavities 213 arranged in sequence, and the plurality of first mounting cavities 213 extend to the rear end face of the front light barrel 210, and the inner diameters increase in sequence, so as to facilitate the installation of the optical devices of the aforementioned front light optical module into the front light barrel 210 in sequence.

[0205] In some embodiments, referring to FIG5 , front light channel 211 further includes a second mounting cavity 214 extending to the front end of front light barrel 210. Second mounting cavity 214 communicates with first mounting cavity 213 via an intermediate cavity. Light source 700 is at least partially located in second mounting cavity 214, thereby connecting light source 700 to front light barrel 210.

[0206] A fifth embodiment of the present invention also provides a sample analyzer, as shown in FIG10 . The sample analyzer includes a sampling device 1, a sample preparation device 2, a particle detection device 3, a display device 4, and a control device 5. The sampling device 1 is used to obtain a sample to be tested; the sample preparation device 2 is used to receive the sample to be tested obtained by the sampling device 1 and mix the sample to be tested with a reagent to prepare a sample solution to be tested; the particle detection device 3 is used to test the sample solution prepared by the sample preparation device 2 to obtain routine blood test parameters; and the display device 4 is used to display information related to the routine blood test parameters. For example, the display device 4 is configured as a user interface. The control device 5 includes a processor and a storage medium storing a computer program. In addition to the aforementioned main components, the sample analyzer may also include a first housing 6 and a second housing 7. The particle detection device 3 and the control device 5 are disposed within the second housing 7, on either side of the second housing 7. The sample preparation device 2 is disposed within the first housing 6. The display device 4 is disposed on an outer surface of the first housing 6.

[0207] The particle detection device 3 of this embodiment can adopt the particle detection device of the aforementioned first embodiment and other specific embodiments based on the first embodiment, specifically including a main lens barrel 100, a front light component 200, a rear light component 300, a flow chamber component 400, a light source 700 and a light receiving component. The main lens barrel 100 has a main channel 110, and the front light component 200 also includes a front light lens barrel 210. The front light optical module is connected to the front light lens barrel 210, and the front light lens barrel 210 is nested and connected to the main lens barrel 100. In this way, the displacement between the front light lens barrel 210 and the main lens barrel 100 in the radial plane is limited by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the inner circle and outer circle of the front light lens barrel 210 and the main lens barrel 100 to achieve precise radial positioning.

[0208] In addition, in this embodiment, the front light assembly 200 includes a single front light lens barrel 210, and the main lens barrel 100 is nested and connected with the single front light lens barrel 210. In other words, the front light optical module is indirectly connected to the main lens barrel 100 through the front light lens barrel 210. In this way, the front light assembly 200 can be pre-assembled separately, and then the front light lens barrel 210 is nested and connected with the main lens barrel 100, which is convenient for assembly.

[0209] A sixth embodiment of the present invention also provides a sample analyzer, as shown in FIG10 . The sample analyzer includes a sampling device 1, a sample preparation device 2, a particle detection device 3, a display device 4, and a control device 5. The sampling device 1 is used to obtain a sample to be tested; the sample preparation device 2 is used to receive the sample to be tested obtained by the sampling device 1 and mix the sample to be tested with a reagent to prepare a sample solution to be tested; the particle detection device 3 is used to test the sample solution prepared by the sample preparation device 2 to obtain routine blood test parameters; and the display device 4 is used to display information related to the routine blood test parameters. For example, the display device 4 is configured as a user interface. The control device 5 includes a processor and a storage medium storing a computer program. In addition to the aforementioned main components, the sample analyzer may also include a first housing 6 and a second housing 7. The particle detection device 3 and the control device 5 are disposed within the second housing 7, on either side of the second housing 7. The sample preparation device 2 is disposed within the first housing 6. The display device 4 is disposed on the outer surface of the first housing 6.

[0210] The particle detection device 3 of this embodiment can adopt the particle detection device of the aforementioned second embodiment and other specific embodiments based on the second embodiment, specifically including a main body barrel 100, a front light component 200, a rear light component 300, a flow chamber component 400, a light source 700 and a light receiving component. The main body barrel 100 has a main channel 110, and the rear light component 300 also includes a rear light barrel 310. The rear light optical module is connected to the rear light barrel 310, and the rear light barrel 310 is nested and connected to the main body barrel 100. In this way, the displacement between the rear light barrel 310 and the main body barrel 100 in the radial plane is limited by the mutually abutting barrel walls. Therefore, it is only necessary to ensure the accuracy of the inner circle and outer circle of the rear light barrel 310 and the main body barrel 100 to achieve precise radial positioning.

[0211] A seventh embodiment of the present invention also provides a sample analyzer, as shown in FIG10 . The sample analyzer includes a sampling device 1, a sample preparation device 2, a particle detection device 3, a display device 4, and a control device 5. The sampling device 1 is used to obtain a sample to be tested. The sample preparation device 2 is used to receive the sample to be tested obtained by the sampling device 1 and mix the sample to be tested with a reagent to prepare a sample solution to be tested. The particle detection device 3 is used to test the sample solution prepared by the sample preparation device 2 to obtain routine blood test parameters. The display device 4 is used to display information related to the routine blood test parameters. For example, the display device 4 is configured as a user interface. The control device 5 includes a processor and a storage medium storing a computer program. In addition to the aforementioned main components, the sample analyzer may also include a first housing 6 and a second housing 7. The particle detection device 3 and the control device 5 are disposed within the second housing 7, on either side of the second housing 7. The sample preparation device 2 is disposed within the first housing 6. The display device 4 is disposed on the outer surface of the first housing 6.

[0212] The particle detection device 3 of this embodiment can adopt the particle detection device of the aforementioned third embodiment and other specific embodiments based on the third embodiment, and specifically includes a main body barrel 100, a front light assembly 200, a back light assembly 300, a flow chamber assembly 400, a light source 700, and a light receiving assembly. The front light assembly 200 includes a front light barrel 210 and a front light optical module, which is connected to the front light barrel 210. The back light assembly 300 includes a back light barrel 310 and a back light optical module, which is connected to the back light barrel 310.

[0213] The flow cell assembly 400 includes an injection section 410, a detection section 420, and a discharge section 430, which are sequentially arranged along the flow direction of the sample liquid. The injection section 410 is used to allow the sample liquid to flow into the detection section 420. At least the detection section 420 of the flow cell assembly 400 is located in the main channel 110. Along the flow direction of the sample liquid, the central axis of the main channel 110 is located at both ends of the detection section 420. That is, when the flow cell assembly 400, the main barrel 100, the front light assembly 200, and the rear light assembly 300 are assembled, the detection section 420, the front light assembly 200, and the rear light assembly 300 are positioned in the flow direction of the sample liquid. This ensures that the illumination light emitted by the front light assembly 200 can illuminate the sample liquid in the detection section 420, and the illumination light emitted by the detection section 420 can also accurately pass through the rear light optical module. This eliminates the need to adjust the flow cell assembly 400 in the flow direction of the sample liquid, simplifying assembly and commissioning.

[0214] While the embodiments of the present invention have been described in detail above with reference to the accompanying drawings, the present invention is not limited to the embodiments described above. Various modifications may be made within the scope of knowledge possessed by a person skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof may be combined with one another unless there is a conflict.

Claims

1. A particle detection device, characterized in that include: a light source for providing irradiation light; A front light assembly includes a single front light barrel and a front light optical module, wherein the single front light barrel includes a front light channel, the front light optical module is disposed in the front light channel, the irradiation light passes through the front light optical module, and the front light optical module shapes the irradiation light; a main body lens barrel having a main body channel, through which the illumination light shaped by the front light optical module passes; a flow cell assembly connected to the main lens barrel, the flow cell assembly having a detection portion for allowing a sample liquid to pass therethrough, the detection portion including an incident side disposed toward the front light assembly and an exit side disposed opposite the incident side, the illumination light shaped by the front light optical module entering the detection portion from the incident side and exiting from the exit side after irradiating the sample liquid to be tested; a backlight assembly, comprising a backlight optical module, the backlight optical module shaping the illumination light emitted from the emission side of the detection portion; a light receiving component for receiving the illumination light shaped by the rear light component; The cross section of the main body channel is circular, and the single front light barrel is at least partially located in the main body channel so as to be nested and connected with the main body barrel; Alternatively, the cross section of the front light channel is circular, and the main body lens barrel portion is located in the front light channel to be nested and connected with the single front light lens barrel; The central axis of the front light channel, the central axis of the main body channel and the optical axis of the front light optical module are substantially coaxial.

2. The particle detection device according to claim 1, characterized in that The light source and the main body barrel can move relative to each other along the axial direction of the main body channel to adjust the distance between the light source and the flow chamber assembly.

3. The particle detection device according to claim 2, characterized in that The light source is connected to the single front light barrel, wherein: The single front light lens barrel, the light source and the front light optical module can be synchronously moved relative to the main body lens barrel along the axial direction of the main body channel; Alternatively, the light source is movable relative to the single front light barrel along the axial direction of the main body channel.

4. The particle detection device according to claim 3, characterized in that The particle detection device also includes a limit member, which is connected to one of the single front light lens barrel and the main body lens barrel, and can act on the other of the two to maintain the relative position between the single front light lens barrel and the main body lens barrel along the axial direction of the main channel, and / or to limit the relative rotation between the single front light lens barrel and the main body lens barrel along the circumference of the main channel or the circumference of the front light channel.

5. The particle detection device according to claim 4, characterized in that The other of the single front light lens barrel and the main body lens barrel has a limit groove, and the distance between the two side walls of the limit groove gradually decreases along the radial direction pointing to the central axis of the main body channel. The limit member is inserted into the limit groove, and the limit member abuts against the two side walls of the limit groove.

6. The particle detection device according to claim 1, characterized in that The backlight optical module includes a first condensing lens and a first aperture, and the light receiving component is used to receive the irradiation light emitted from the exit side of the detection unit and passing through the first condensing lens and the first aperture; The particle detection device further includes a pressing member connected to the main body barrel, and the first aperture is clamped between the main body barrel and the pressing member.

7. The particle detection device according to claim 6, characterized in that The first condensing lens is connected to the main barrel and is located between the detection unit and the first aperture.

8. The particle detection device according to claim 6, characterized in that The pressing member is configured as a rear light barrel, the first condensing lens and the light receiving assembly are both connected to the rear light barrel, and the first condensing lens is located between the first aperture and the light receiving assembly.

9. The particle detection device according to claim 6, characterized in that The pressing member is configured as a rear light lens barrel, and the rear light lens barrel has at least a first rear light channel, wherein: When the cross section of the main body channel is circular, the rear light barrel is at least partially located in the main body channel to be nested and connected with the main body barrel; Alternatively, the cross section of the first rear light channel is circular, and the main body lens barrel is partially located in the first rear light channel to be nested and connected with the rear light lens barrel; The first rear light channel is used to pass the illumination light emitted from the exit side of the detection part, and the central axis of the first rear light channel, the central axis of the main channel, the central axis of the front light channel and the optical axis of the front light optical module are roughly coaxial.

10. The particle detection device according to claim 6, characterized in that The main channel includes a first channel section and a second channel section, and the first channel section and the second channel section are arranged in sequence along the axial direction of the main channel. The diameter of the first channel section is smaller than the diameter of the second channel section to form an abutment surface at the intersection of the two. The pressing piece is inserted into the second channel section to press the first aperture against the abutment surface.

11. The particle detection device according to claim 10, characterized in that The wall of the second channel section is provided with a first hole penetrating to the outer surface of the main body barrel, and the first hole is used for allowing the first aperture to move relative to the main body barrel along the penetrating direction of the first hole.

12. The particle detection device according to claim 6, characterized in that The pressing member is detachably connected to the main barrel, and when the pressing member is in a non-pressing state, the first aperture is configured to be movable relative to the main barrel along a first direction, wherein the first direction is parallel to a radial direction of the main channel perpendicular to a flow direction of the sample liquid to be tested.

13. The particle detection device according to claim 12, characterized in that: One of the main barrel and the first diaphragm is provided with a first positioning portion, and the other is provided with a second positioning portion, and the first diaphragm can be moved along the first direction by the first positioning portion and the second positioning portion; Alternatively, one of the pressing member and the first aperture is provided with a first positioning portion, and the other is provided with a second positioning portion, and the first aperture can be moved along the first direction through the first positioning portion and the second positioning portion.

14. The particle detection device according to claim 1, wherein The rear light assembly further includes a rear light barrel, the rear light barrel having at least a first rear light channel, the central axis of the first rear light channel, the central axis of the main body channel, the central axis of the front light channel, and the optical axis of the front light optical module being substantially coaxial, the rear light optical module including a first condensing lens and a first aperture, the light receiving assembly being configured to receive the irradiation light emitted from the exit side of the detection portion and passing through the first condensing lens and the first aperture, wherein the first condensing lens and the first aperture are disposed in the first rear light channel, and the light receiving assemblies are both connected to the rear light barrel, wherein: When the cross section of the main body channel is circular, the rear light barrel is at least partially located in the main body channel to be nested and connected with the main body barrel; Alternatively, the cross section of the first rear light channel is circular, and the main body lens barrel portion is located in the first rear light channel to be nested and connected with the rear light lens barrel.

15. The particle detection device according to claim 1, wherein The rear light optical module includes a first condensing lens and a first aperture, and the light receiving component is used to receive the irradiation light emitted from the exit side of the detection unit and passing through the first condensing lens and the first aperture, wherein the first condensing lens, the first aperture and the light receiving component are all connected to the main lens barrel.

16. The particle detection device according to claim 15, characterized in that The wall of the main channel is provided with a first hole penetrating to the outer surface of the main barrel, and the first aperture is configured to be movable relative to the main barrel along a penetrating direction of the first hole through the first hole.

17. The particle detection device according to claim 16, characterized in that At least one end of the first aperture extends from the first hole. The particle detection device further includes a fixing member, which acts on the extending portion of the first aperture to fix the first aperture to the main body barrel.

18. The particle detection device according to claim 1, wherein The rear light assembly further includes a rear light barrel, the rear light barrel having at least a first rear light channel, the first rear light channel being used to pass the illumination light emitted from the exit side of the detection portion, the central axis of the first rear light channel, the central axis of the main body channel, the central axis of the front light channel, and the optical axis of the front light optical module being substantially coaxial, and the rear light optical module being at least partially connected to the rear light barrel; Alternatively, the rear light assembly includes a rear light optical module, and all of the rear light optical modules are connected to the main lens barrel.

19. The particle detection device according to claim 1, wherein The flow chamber assembly further includes a liquid injection portion and a liquid discharge portion, wherein the liquid injection portion, the detection portion and the liquid discharge portion are sequentially arranged along the flow direction of the sample liquid to be tested, wherein at least the detection portion is located in the main channel.

20. The particle detection device according to claim 1, wherein The flow chamber assembly is connected to the main barrel and is configured to be movable relative to the main barrel along a first direction, wherein the first direction is parallel to a radial direction of the main channel perpendicular to a flow direction of the sample liquid to be tested.

21. The particle detection device according to claim 20, characterized in that One of the main barrel and the flow chamber assembly is provided with a third positioning portion, and the other is provided with a fourth positioning portion. The flow chamber assembly can move relative to the main barrel along the first direction through the third positioning portion and the fourth positioning portion.

22. The particle detection device according to claim 1, characterized in that The rear light optical module includes a first condensing lens and a reflector, and the light receiving assembly includes a first light receiving device and a second light receiving device; The first condenser lens is configured to converge first illumination light emitted from the exit side of the detection unit and within a first angle range, second illumination light emitted from the exit side of the detection unit and within a second angle range, and third illumination light emitted from the exit side of the detection unit and within a third angle range; The reflector is used to reflect the light formed by the second illumination light after being converged by the first condensing lens; The first light receiving device is used to receive light formed by the first illumination light being converged by the first condensing lens, and light formed by the third illumination light being converged by the first condensing lens, and the second light receiving device is used to receive light formed by the second illumination light being converged by the first condensing lens and reflected by the reflector.

23. The particle detection device according to claim 22, characterized in that Each angle in the second angle range is smaller than each angle in the first angle range, and each angle in the third angle range is larger than each angle in the second angle range and smaller than each angle in the first angle range.

24. The particle detection device according to claim 22, characterized in that The first light receiving device and the second light receiving device both have a light receiving area, and the light receiving areas of the first light receiving device and the second light receiving device are oriented in the same direction. Along the axial direction of the main channel, the distance between the light receiving area of ​​the first light receiving device and the first focusing lens is smaller than the distance between the light receiving area of ​​the second light receiving device and the first focusing lens.

25. The particle detection device according to claim 1, characterized in that The front light optical module includes a cylindrical lens and a second condensing lens sequentially arranged along the axial direction of the front light channel, and the cylindrical lens and the second condensing lens are located in the front light channel.

26. The particle detection device according to claim 25, characterized in that The front light optical module further includes an aspheric lens, which is located in the front light channel and between the light source and the cylindrical lens.

27. The particle detection device according to claim 26, characterized in that The front light optical module further includes an optical isolator, which is located in the front light channel and between the aspheric lens and the cylindrical lens; And / or, the front light optical module further includes a second aperture, which is located in the front light channel and between the light source and the aspheric lens.

28. The particle detection device according to claim 1, characterized in that Along the axial direction of the front light channel, the front light channel includes a plurality of first installation cavities which are sequentially arranged and extend to the rear end surface of the front light lens barrel, and the inner diameter of each of the first installation cavities increases sequentially.

29. The particle detection device according to claim 28, characterized in that The front light channel further includes a second mounting cavity, which extends to the front end surface of the front light lens barrel, and the light source is at least partially located in the second mounting cavity.

30. A sample analyzer, characterized in that include: A sampling device for obtaining a sample to be tested; a sample preparation device, configured to receive the sample to be tested obtained by the sampling device, and mix the sample to be tested with a reagent to prepare a sample liquid to be tested; The particle detection device according to any one of claims 1 to 29; a liquid supply device for supplying sheath fluid to the flow cell assembly, so that particles in the sample liquid to be tested are carried by the sheath fluid and arranged in a queue in the detection portion; The controller is configured to obtain a measurement result of the sample to be measured according to the feedback signal received by the light receiving component.

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