Flea repellent and application thereof

Abstract

The invention provides a flea repellent and application thereof. The flea repellent comprises nerolidol, eudesmol and / or linalool, the nerolidol, eudesmol and / or linalool of the flea repellent are compounded, and compared with single nerolidol, the flea repellent has a better repelling effect on flea; the flea repellent disclosed by the invention can be used for replacing a traditional repellent DEET. According to the flea repellent disclosed by the invention, when nerolidol, eudesmol and linalool are compounded according to a ratio of 1: 1: 1, the repellent rate can reach 85.6% at most within 5 minutes.

Description

Technical Field

[0001] The invention relates to the technical field of pest control, and in particular to a flea repellent and application thereof. Background Art

[0002] Fleas (order: Siphonoptera) are blood-sucking, wingless insects, including 246 genera and more than 2,500 species. In my country, there are 35 known hosts of fleas besides humans, with humans, domestic dogs and domestic cats being the most common. As carriers of a variety of pathogens, fleas transmit their own pathogens to the host when sucking blood, which can not only cause a variety of zoonotic diseases including Rickettsia, Bartonella, Yersinia pestis and other bacterial diseases. Fleas can also cause allergic reactions in animals when biting them, causing extreme itching. This allergic reaction not only makes animals feel uncomfortable, but can also cause scratching and hair loss, increasing the risk of secondary infection. In summary, flea bites not only cause various health problems in animals themselves, but also spread diseases to humans through them as vectors, so the control and prevention of fleas is crucial. In ancient times, people used burning plant leaves or applying some herbal ingredients to repel mosquitoes. Plants can synthesize some secondary metabolites to protect themselves from pathogens and pests. Historically, plants used to repel mosquitoes include chrysanthemums and pyrethrum. Later, products widely used for personal protection were DEET (DEET) and permethrin. At present, common repellents include DEET, N,N-diethylphenylacetamide (DEPA), etc. DEET is a safe and broad-spectrum repellent widely used in humans and animals worldwide. However, some studies have also reported the toxicity associated with the use of DEET, especially when abused in large doses, which may involve health threats and even immediate symptoms such as movement disorders, tremors, and fainting.

[0003] Based on the defects of the current repellent DEET, it is necessary to improve it. Summary of the invention

[0004] In view of this, the present invention provides a flea repellent and application thereof to solve or partially solve the problems existing in the prior art.

[0005] In a first aspect, the present invention provides a flea repellent comprising nerolidol, eucalyptol and / or linalool.

[0006] Preferably, it comprises nerolidol and eucalyptol, and the volume ratio of nerolidol to eucalyptol is (1-3):(1-3).

[0007] Preferably, it comprises nerolidol and linalool, and the volume ratio of nerolidol to linalool is (1-3):(1-3).

[0008] Preferably, it comprises nerolidol, eucalyptol and linalool, wherein the ratio of nerolidol, eucalyptol and linalool is (1-3):(1-3):(1-3).

[0009] Preferably, a solvent is also included.

[0010] Preferably, the solvent is ethanol.

[0011] Preferably, it comprises nerolidol, eucalyptol and ethanol, the volume ratio of nerolidol to eucalyptol is (1-3):(1-3), and the sum of the volume concentrations of nerolidol and eucalyptol is 20-40%.

[0012] Preferably, it comprises nerolidol, linalool and ethanol, the volume ratio of nerolidol to linalool is (1-3):(1-3), and the sum of the volume concentrations of nerolidol and linalool is 20-40%.

[0013] Preferably, it comprises nerolidol, eucalyptol, linalool and ethanol, wherein the ratio of nerolidol, eucalyptol and linalool is (1-3):(1-3):(1-3), and the sum of the volume concentrations of nerolidol, eucalyptol and linalool is 20-40%.

[0014] In a second aspect, the present invention also provides a use of the flea repellent in the preparation of a flea repellent drug.

[0015] The flea repellent of the present invention and its application have the following beneficial effects compared with the prior art:

[0016] 1. The flea repellent of the present invention comprises nerolidol, eucalyptol and / or linalool. The flea repellent of the present invention has a better flea repelling effect on fleas than single nerolidol by compounding nerolidol, eucalyptol and / or linalool. The flea repellent of the present invention can replace the traditional repellent DEET.

[0017] 2. The flea repellent of the present invention, when nerolidol, eucalyptol and linalool are compounded in a ratio of 1:1:1, has a maximum repellency rate of 85.6% within 5 minutes. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is the connection diagram of the Y-type olfactometer. DETAILED DESCRIPTION

[0019] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] It should be noted that the order of description of the following embodiments is not intended to limit the preferred order of the embodiments. In addition, in the description of the present application, the term "including" means "including but not limited to". Various embodiments of the present invention may be in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity and should not be understood as a rigid limitation on the scope of the present invention; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the numbered ranges, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated in this article, it is meant to include any cited numbers (fractions or integers) within the indicated range.

[0021] The embodiments of the present application provide a flea repellent, comprising nerolidol, eucalyptol and / or linalool.

[0022] The flea repellent of the present invention is compounded with nerolidol, eucalyptol and / or linalool, and has a better repellent effect on fleas than single nerolidol.

[0023] In some embodiments, the flea repellent comprises nerolidol and eucalyptol, and the volume ratio of nerolidol to eucalyptol is (1-3):(1-3).

[0024] In some embodiments, the flea repellent comprises nerolidol and linalool, and the volume ratio of nerolidol to linalool is (1-3):(1-3).

[0025] In some embodiments, the flea repellent comprises nerolidol, eucalyptol and linalool, (1-3) of nerolidol, eucalyptol and linalool:(1-3):(1-3).

[0026] In some embodiments, the flea repellent further includes a solvent.

[0027] In some embodiments, the flea repellent, the solvent is ethanol.

[0028] In some embodiments, the flea repellent comprises nerolidol, eucalyptol and ethanol, the volume ratio of nerolidol to eucalyptol is (1-3):(1-3), and the sum of the volume concentrations of nerolidol and eucalyptol is 20-40%.

[0029] In some embodiments, the flea repellent comprises nerolidol, linalool and ethanol, the volume ratio of nerolidol to linalool is (1-3):(1-3), and the sum of the volume concentrations of nerolidol and linalool is 20-40%.

[0030] In some embodiments, the flea repellent includes nerolidol, eucalyptol, linalool and ethanol, the ratio of nerolidol, eucalyptol and linalool is (1-3):(1-3):(1-3), and the sum of the volume concentrations of nerolidol, eucalyptol and linalool is 20-40%.

[0031] Based on the same inventive concept, the present invention also provides the use of the above-mentioned flea repellent in the preparation of flea repellent medicine.

[0032] The flea repellent of the present invention and its preparation method and application are further described below with specific examples. This section further illustrates the content of the present invention in conjunction with specific examples, but should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0033] Example 1

[0034] This embodiment provides a flea repellent, comprising nerolidol, linalool and ethanol, the volume ratio of nerolidol to linalool is 1:1, and the sum of the volume concentrations of nerolidol and linalool is 40%.

[0035] The preparation method of the flea repellent is as follows: nerolidol and linalool are added into ethanol to obtain the flea repellent.

[0036] Example 2

[0037] This embodiment provides a flea repellent, comprising nerolidol, eucalyptol and ethanol, the volume ratio of nerolidol to eucalyptol is 1:1, and the sum of the volume concentrations of nerolidol and eucalyptol is 40%.

[0038] The preparation method of the flea repellent is as follows: nerolidol and eucalyptol are added into ethanol to obtain the flea repellent.

[0039] Example 3

[0040] This embodiment provides a flea repellent, including nerolidol, eucalyptol, linalool and ethanol, the volume ratio of nerolidol, eucalyptol and linalool is 1:1:1, and the sum of the volume concentrations of nerolidol, eucalyptol and linalool is 40%.

[0041] The preparation method of the flea repellent is as follows: nerolidol, eucalyptol and linalool are added into ethanol to obtain the flea repellent.

[0042] Comparative Example 1

[0043] The flea repellent provided in this comparative example comprises nerolidol and ethanol, and the volume concentration of nerolidol is 40%.

[0044] The preparation method of the flea repellent is as follows: nerolidol is added into ethanol to obtain the flea repellent.

[0045] Comparative Example 2

[0046] The flea repellent provided in this comparative example comprises nerolidol and ethanol, and the volume concentration of nerolidol is 30%.

[0047] The preparation method of the flea repellent is as follows: nerolidol is added into ethanol to obtain the flea repellent.

[0048] Comparative Example 3

[0049] The repellent provided in this comparative example is DEET, and its preparation method is: DEET is added into ethanol so that the volume concentration of DEET is 40%, thereby obtaining the repellent DEET.

[0050] Performance Testing

[0051] Repellent activity test of flea repellents

[0052] The repellent activity against fleas was determined by using a "Y" type olfactometer method. The measuring device consists of an atmospheric sampler (which is provided with an air inlet and an air outlet), activated carbon, a humidifier, a "Y" type tube, a drying bottle, a vacuum pump, a water bottle, a sample bottle and an odorless silicone tube. The "Y" type tube is a glass instrument with a middle long tube of 20 cm long, two arm tubes of 10 cm long, and a tube diameter of 5 cm. The connection diagram of the Y type olfactometer is shown in Figure 1 .

[0053] Each group of tests selected 30 fleas to measure their reaction to essential oils. Cut filter paper with a diameter of 7 mm, absorb 0.2 μL of the test sample (i.e., the flea repellent in different embodiments and comparative examples) onto the filter paper, and quickly place it in the A branch of the "Y"-shaped tube (for the convenience of experimental data recording), and place a blank filter paper strip in the other side of the B branch as a control group. After the filter paper strip is placed in the A branch or the B branch, turn on the vacuum pump device, and the outside air passes through the air inlet to the activated carbon for deodorization and purification, and then enters the humidification device filled with pure water to moisten, and then enters the Y-shaped tube through the sample bottle, and finally is discharged to the outside through the silicone tube. The gas flow rate of the entire experiment is controlled at 20 mL / min.

[0054] Each group of experiments was repeated 3 times, and the number of each repellent staying in the two-arm tubes was recorded at the 5-minute time point under the reagent volume of 0.2 μL. The results of the 3 experiments were averaged. In each group of experiments, one tube wall was left as a blank control. Each group of experiments was repeated at least 3 times. After the experiment, the odor selection device, silicone tube, and sample measurement cylinder were soaked in detergent for 40 to 50 minutes, then wiped clean with absorbent paper, soaked in clean water for 30 to 40 minutes, and dried naturally for use.

[0055] The experimental results were analyzed and calculated using SPSS16.0 software.

[0056] The calculation formula for repellency rate (%) is: PR% = (Nc-Nt) / (Nc+Nt)×100%. In the above formula, Nc represents the number of mosquitoes in the control branch (i.e., branch B), and Nt represents the number of mosquitoes in the treatment branch (i.e., branch A).

[0057] The repellency test results are shown in Table 1 below.

[0058] Table 1 - Repellent activity of different repellents

[0059]

[0060] According to the nerolidol + linalool, nerolidol + eucalyptol, nerolidol + eucalyptol + linalool in Examples 1 to 3, the concentration was 40%, and DEET was used as a comparison. The repellency rate was measured for 5 minutes, as shown in Table 1. The highest repellency rate within 5 minutes was 85.6% in Example 3. It can be seen from Table 1 that compared with single nerolidol, the combination of nerolidol, eucalyptol and / or linalool has a better repellent effect on fleas.

[0061] It can be understood that the technical features of the above-described embodiments can be arbitrarily combined. In order to make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0062] The above are only preferred embodiments of the present application, and only specifically describe the technical principles of the present application. These descriptions are only for explaining the principles of the present application and cannot be interpreted as limiting the scope of protection of the present application in any way. Based on the explanation here, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application, and other specific implementation methods of the present application that can be associated with the technicians in this field without creative work, should be included in the scope of protection of the present application.

Claims

1. A flea repellent, characterized in that: Includes nerolidol, eucalyptol and / or linalool.

2. The flea repellent according to claim 1, characterized in that The invention comprises nerolidol and eucalyptol, wherein the volume ratio of nerolidol to eucalyptol is (1-3):(1-3).

3. The flea repellent according to claim 1, characterized in that The invention comprises nerolidol and linalool, wherein the volume ratio of nerolidol to linalool is (1-3):(1-3).

4. The flea repellent according to claim 1, characterized in that It includes nerolidol, eucalyptol and linalool, wherein the nerolidol, eucalyptol and linalool are (1-3):(1-3):(1-3).

5. The flea repellent according to any one of claims 1 to 4, characterized in that Also included are solvents.

6. The flea repellent according to claim 5, characterized in that The solvent is ethanol.

7. The flea repellent according to claim 6, characterized in that The invention comprises nerolidol, eucalyptol and ethanol, wherein the volume ratio of nerolidol to eucalyptol is (1-3):(1-3), and the sum of the volume concentrations of nerolidol and eucalyptol is 20-40%.

8. The flea repellent according to claim 6, characterized in that The invention comprises nerolidol, linalool and ethanol, wherein the volume ratio of nerolidol to linalool is (1-3):(1-3), and the sum of the volume concentrations of nerolidol and linalool is 20-40%.

9. The flea repellent according to claim 6, characterized in that The invention comprises nerolidol, eucalyptol, linalool and ethanol, wherein the ratio of nerolidol, eucalyptol and linalool is (1-3):(1-3):(1-3), and the sum of the volume concentrations of nerolidol, eucalyptol and linalool is 20-40%.

10. Use of the flea repellent according to any one of claims 1 to 9 in the preparation of a flea repellent drug.

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