A firming anti-wrinkle composition and method of making and using same

Abstract

The application discloses a firming and anti-wrinkle composition and a preparation method and application thereof, and belongs to the technical field of cosmetics. The firming and anti-wrinkle composition comprises the following components in mass parts: 0.0001-0.005 parts of platinum powder, 0.0001-18 parts of palmitoyl pentapeptide-4, 0.0001-10 parts of musclease, 0.0001-1 part of Buxbaumia aphylla extract and 0.0001-3 parts of guettarda speciosa leaf extract. The firming and anti-wrinkle composition has good interaction between the components by selecting the components in the specific mass part range, can effectively firm the skin, increase the skin elasticity, reduce the generation of skin wrinkles when applied to cosmetics, and has excellent moisturizing effect and skin lightening effect. Meanwhile, the preparation method of the firming and anti-wrinkle composition is simple in operation, the raw materials are widely sourced, and is beneficial to actual production.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a firming and anti-wrinkle composition as well as a preparation method and application thereof. BACKGROUND

[0002] With the increase of age and the gradual deterioration of the environment and other factors, the skin of a person will gradually become loose, and wrinkles will also increase; at the same time, the collagen in the facial tissue will decrease, resulting in loss of elasticity of the skin and generation of wrinkles, and the moisture content of the skin is greatly reduced.

[0003] Nowadays, people have a high pursuit of firming and anti-wrinkle, and although there are many products with firming and anti-wrinkle effects in the prior art, in order to meet the increasing requirements of people, the efficacy of cosmetics also needs to be further improved. SUMMARY

[0004] The present application aims to overcome the deficiencies of the prior art and provide a firming and anti-wrinkle composition which can effectively remove wrinkles, firm the skin, increase the elasticity of the skin, and also has good moisturizing effect and skin lightening effect, as well as a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, in the first aspect of the present application, the present application provides a firming and anti-wrinkle composition, which comprises the following components by weight: 0.0001-0.005 parts of platinum powder, 0.0001-18 parts of palmitoyl pentapeptide-4, 0.0001-10 parts of musclease, 0.0001-1 part of Balkan primrose extract, and 0.0001-3 parts of guava leaf extract.

[0006] The firming and anti-wrinkle composition provided by the present application has good compounding effect between components by selecting components within a specific mass fraction range, and can effectively firm the skin, increase the elasticity of the skin, reduce the generation of skin wrinkles, and also has excellent moisturizing effect and skin lightening effect when applied to cosmetics.

[0007] Specifically, the present application researches and finds that the platinum powder has good antioxidant effect, can also soothe the skin, reduce the discomfort symptoms such as skin redness and swelling, and can also provide luster to the skin and enhance the luster of the skin; palmitoyl pentapeptide-4 can stimulate the synthesis of collagen, glycosaminoglycan and hyaluronic acid, stimulate fibroblast growth, effectively reduce skin fine lines, improve wrinkles, enhance the tightness and luster of the skin; the muscle peptide is a good stabilizer of cell membrane, has the effect of protecting the cell membrane, also has good antioxidant effect, removes excess free radicals in the skin, reduces skin aging, increases skin elasticity, and also can brighten the skin and enhance the luster of the skin; the genus Procrasteroglossia is called resurrection grass, which has good repair function and super water supplement ability, the genus Procrasteroglossia extract can rebuild, activate and moisturize the skin, increase the elasticity and moisture retention of the skin, and further enhance the luster of the skin; psidium guajava leaf extract not only can whiten the skin, but also has good antioxidant effect, which can help increase skin elasticity. Further, the present application researches and finds that the five components selected by the present application have good compounding effect, for example, the genus Procrasteroglossia extract provided by the present application not only can increase skin elasticity, but also has excellent and long-lasting moisturizing effect, which can help other substances to be better absorbed, thereby achieving more excellent effect; in addition, the platinum powder in the present application can effectively activate the skin, and synergize with palmitoyl pentapeptide-4 and muscle peptide to effectively enhance the effect of palmitoyl pentapeptide-4 and muscle peptide on improving skin elasticity, and further promote the long-acting moisturizing effect of the genus Procrasteroglossia extract; in addition, the psidium guajava leaf extract can also interact with the platinum powder and other components to achieve more excellent antioxidant effect and skin lightening effect, thereby reducing the generation of wrinkles and improving the luster of the skin from the source.

[0008] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, the firming and anti-wrinkle composition comprises the following components in parts by weight: 0.0005-0.002 platinum powder, 1-5 palmitoyl pentapeptide-4, 0.05-0.4 muscle peptide, 0.1-0.5 genus Procrasteroglossia extract, 0.3-1.4 psidium guajava leaf extract.

[0009] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, the firming and anti-wrinkle composition comprises the following components in parts by weight: 0.001-0.0015 platinum powder, 2-3 palmitoyl pentapeptide-4, 0.2-0.3 muscle peptide, 0.2-0.3 genus Procrasteroglossia extract, 0.5-0.8 psidium guajava leaf extract.

[0010] The present application researches and finds that the mass fraction of the components in the firming and anti-wrinkle composition can affect the comprehensive effect of the firming and anti-wrinkle composition, and when the mass fraction of the components is further selected within the above range, the firming and anti-wrinkle composition obtained is applied to cosmetics, and the firming effect, moisturizing effect, wrinkle-removing effect and skin lightening effect of the obtained cosmetics are all more excellent.

[0011] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, in the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of balkan borage extract is (10-14):1.

[0012] Illustratively, in the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of balkan borage extract can be any point value or any two-point range value between (10-14):1, such as 10:1, 11:1, 12:1, 13:1, 14:1, etc.

[0013] The present application researches and finds that the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of balkan borage extract has an influence on the moisturizing effect, skin elasticity increase and wrinkle-removing effect of the product, and when the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of balkan borage extract is further selected to be (10-14):1, the comprehensive effect of the obtained product is more excellent.

[0014] Preferably, in the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the mass of balkan borage extract is (11-12):1.

[0015] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, in the firming and anti-wrinkle composition, the mass percentage of psidium guajava leaf extract is 5-50%.

[0016] Illustratively, in the firming and anti-wrinkle composition, the mass percentage of psidium guajava leaf extract can be any point value or any two-point range value within 5-50%, such as 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, etc.

[0017] The present application researches and finds that the mass percentage of psidium guajava leaf extract in the firming and anti-wrinkle composition can affect the comprehensive performance of the product, and when the mass percentage of psidium guajava leaf extract is further selected within the above range, the obtained product has more excellent anti-wrinkle performance and more excellent moisturizing effect.

[0018] Preferably, in the firming and anti-wrinkle composition, the mass percentage of psidium guajava leaf extract is 15-20%.

[0019] As a preferred embodiment of the firming anti-wrinkle composition of the present application, the average particle size of the platinum powder is 0.15-0.50 μm.

[0020] Preferably, the average particle size of the platinum powder is 0.2-0.3 μm.

[0021] Illustratively, the average particle size of the platinum powder can be any point value or any two-point range value between 0.15-0.40 μm, such as 0.15 μm, 0.17 μm, 0.19 μm, 0.2 μm, 0.22 μm, 0.24 μm, 0.26 μm, 0.28 μm, 0.3 μm, 0.32 μm, 0.34 μm, 0.36 μm, 0.38 μm, 0.4 μm, 0.42 μm, 0.44 μm, 0.46 μm, 0.48 μm, 0.5 μm, etc.

[0022] It should be noted that the platinum powder in the specific average particle size range of the present application can be obtained by conventional commercial purchase; or can be obtained by conventional method; the corresponding platinum powder can be obtained by further crushing and screening, so as to obtain the platinum powder in the specific average particle size range.

[0023] The present application researches and finds that the average particle size of the platinum powder not only directly affects the effect of the platinum powder on the skin, but also affects the uniform dispersion effect of the platinum powder and other components, thereby affecting the mutual cooperation of the platinum powder and other components, and further affecting the comprehensive effect of the product; when the average particle size of the platinum powder is further selected to be 0.15-0.50 μm, the comprehensive performance of the obtained product is more excellent; however, considering the preparation and cost factors, the average particle size of the platinum powder is further preferably 0.2-0.3 μm.

[0024] As a preferred embodiment of the firming anti-wrinkle composition of the present application, the preparation method of the Geoclemys reevesii extract comprises the following steps: taking the dried Geoclemys reevesii and crushing, then adding the ethanol aqueous solution for impregnation, ultrasonic extraction after the impregnation is completed, filtration after the extraction is completed, collecting the filtrate and concentrating and drying to obtain the Geoclemys reevesii extract.

[0025] As a preferred embodiment of the firming anti-wrinkle composition of the present application, the temperature of the impregnation is 20-40 ℃, and the time of the impregnation is 12-24 h.

[0026] As a preferred embodiment of the firming anti-wrinkle composition of the present application, the power of the ultrasonic is 120-160 W, the time of the ultrasonic is 10-40 min, and the temperature of the ultrasonic is 40-55 ℃.

[0027] Preferably, the mass percentage of ethanol in the ethanol aqueous solution is 30-60%.

[0028] Preferably, the mass-volume ratio of the P. balkanica and the aqueous ethanol solution is 1g:(6-12)mL.

[0029] The present application studies find that the selection of the preparation method of the P. balkanica extract affects the type and content of the active ingredients therein, and when the preparation method of the P. balkanica extract is further selected as given in the present application, the obtained P. balkanica extract can achieve a more optimal compounding effect with other components, thereby achieving a better comprehensive effect of the product.

[0030] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, the preparation method of the Psidium guajava leaf extract comprises the following steps: taking the dried Psidium guajava leaf, crushing, then enzymolysis, incubation after the end of enzymolysis, obtaining the enzymolysis product, adding the enzymolysis product to the aqueous ethanol solution for extraction, filtering after the end of extraction, collecting the filtrate and concentrating and drying.

[0031] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, in the enzymolysis, the enzyme comprises cellulase and pectinase in a mass ratio of 1:(1-4).

[0032] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, in the enzymolysis, the added mass of the enzyme is 0.1-0.5% of the mass of the Psidium guajava leaf.

[0033] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, in the enzymolysis, the enzymolysis time is 2-4h and the enzymolysis temperature is 30-45℃.

[0034] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, the mass percentage of ethanol in the aqueous ethanol solution is 40-70%.

[0035] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, the extraction temperature is 60-90℃ and the extraction time is 2-6h.

[0036] Preferably, the mass-volume ratio of the enzymolysis product and the aqueous ethanol solution is 1g:(8-15)mL.

[0037] Preferably, the incubation temperature is 80-100℃ and the incubation time is 1-3h.

[0038] The Psidium guajava leaf extract provided by the present application is obtained by enzymolysis followed by heating extraction, which can better extract the corresponding active substances and obtain a higher content of the active substances; when applied to the firming and anti-wrinkle composition, the comprehensive performance of the obtained product is more optimal.

[0039] In the second aspect of the present application, the present application provides a preparation method of the firming and anti-wrinkle composition, which comprises the following steps:

[0040] (1) taking platinum powder into an organic solvent, heating and refluxing, then cooling to room temperature and standing, then collecting the filter residue and drying to obtain pretreated platinum powder;

[0041] (2) mixing the pretreated platinum powder, palmitoyl pentapeptide-4, carnosine, Buxus Perpera extract and Guettarda Krug extract uniformly to obtain the firming and anti-wrinkle composition.

[0042] The firming and anti-wrinkle composition provided by the present application can realize better mixing and dispersion effect between components by pretreating the platinum powder first and then mixing it with the remaining components.

[0043] As a preferred embodiment of the preparation method of the present application, the organic solvent in step (1) comprises at least one of methanol, ethanol, and aqueous ethanol solution.

[0044] As a preferred embodiment of the preparation method of the present application, the heating and refluxing time in step (1) is 1-4h.

[0045] As a preferred embodiment of the preparation method of the present application, the standing time in step (1) is 12-24h.

[0046] In the third aspect of the present application, the present application provides the use of the firming and anti-wrinkle composition in the preparation of cosmetics.

[0047] As a preferred embodiment of the use of the present application, the cosmetics comprise any one of cosmetic water, emulsion, cream, mask, essence, and spray.

[0048] In the fourth aspect of the present application, the present application provides an emulsion comprising the firming and anti-wrinkle composition of the present application.

[0049] As a preferred embodiment of the emulsion of the present application, the emulsion comprises the following components by mass percentage: 1-8% of the firming and anti-wrinkle composition of the present application, 2-40% of a cosmetic base, and the balance of water.

[0050] As a preferred embodiment of the firming and anti-wrinkle composition of the present application, the cosmetic base comprises at least one of thickening agent, humectant, emulsifier, preservative, essence, and pH adjuster.

[0051] Exemplarily, the thickener includes at least one of xanthan gum, carbomer, and sorbitan isostearate; the moisturizer includes at least one of D-panthenol, glycerin, butylene glycol, isononyl isononanoate, squalane, and caprylic / capric triglyceride; the emulsifier includes at least one of cetearyl olivate, sorbitan olivate, coconut oil-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, cetearyl glucoside, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the pH adjuster includes at least one of arginine and disodium EDTA.

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] The firming and anti-wrinkle composition provided by the present invention, by selecting components within a specific mass range and achieving a well-balanced combination, can effectively firm the skin, increase skin elasticity, and reduce the appearance of wrinkles when used in cosmetics. It also exhibits excellent moisturizing and skin-brightening effects. Furthermore, the preparation method of the firming and anti-wrinkle composition provided by the present invention is simple to operate and utilizes a wide range of raw materials, facilitating practical production. DETAILED DESCRIPTION

[0054] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0055] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are all conventional reagents, methods, and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0056] Platinum powder 1: average particle size 0.3 μm, commercially available;

[0057] Platinum powder 2: average particle size 0.2 μm, commercially available;

[0058] Platinum powder 3: average particle size 0.5 μm, commercially available;

[0059] Balkan lettuce extract 1: homemade, the preparation method comprises the following steps: taking Balkan lettuce leaves, drying them, crushing them, and passing them through an 80-mesh sieve, collecting the sieve residue to obtain the crushed Balkan lettuce, then adding 30% ethanol aqueous solution (the mass volume ratio of the crushed Balkan lettuce to the ethanol aqueous solution is 1g:10mL), then immersing them at 35°C for 12h, ultrasonicating them at 120W and 55°C for 15min after the immersion, filtering them after the ultrasonication, collecting the filter residue and concentrating it, and then vacuum drying them at 40°C for 24h to obtain Balkan lettuce extract 1;

[0060] B. balansae extract 2: self-made, the preparation method comprising the following steps: taking dried B. balansae leaves, crushing, and passing through an 80-mesh sieve, collecting the undersize to obtain crushed B. balansae leaves, then adding a 60% ethanol aqueous solution (the mass-volume ratio of the crushed B. balansae leaves to the ethanol aqueous solution is 1 g:6 mL) into the crushed B. balansae leaves, then dipping at 25 °C for 24 h, after the dipping is completed, ultrasonic treatment is carried out at 160 W and 40 °C for 35 min, after the ultrasonic treatment is completed, filtration is carried out, the filter residue is collected and concentrated, then vacuum drying at 40 °C for 24 h, thereby B. balansae extract 2 is obtained;

[0061] B. balansae extract 3: self-made, the preparation method comprising the following steps: taking dried B. balansae leaves, crushing, and passing through an 80-mesh sieve, collecting the undersize to obtain crushed B. balansae leaves, then adding a 30% ethanol aqueous solution (the mass-volume ratio of the crushed B. balansae leaves to the ethanol aqueous solution is 1 g:10 mL) into the crushed B. balansae leaves, then dipping at 35 °C for 14.25 h, after the dipping is completed, filtration is carried out, the filter residue is collected and concentrated, then vacuum drying at 40 °C for 24 h, thereby B. balansae extract 3 is obtained;

[0062] B. balansae extract 4: self-made, the preparation method comprising the following steps: taking dried B. balansae leaves, crushing, and passing through an 80-mesh sieve, collecting the undersize to obtain crushed B. balansae leaves, then adding a 30% ethanol aqueous solution (the mass-volume ratio of the crushed B. balansae leaves to the ethanol aqueous solution is 1 g:10 mL) into the crushed B. balansae leaves, then ultrasonic treatment is carried out at 120 W and 55 °C for 14.25 h, after the ultrasonic treatment is completed, filtration is carried out, the filter residue is collected and concentrated, then vacuum drying at 40 °C for 24 h, thereby B. balansae extract 4 is obtained;

[0063] Psidium guajava leaf extract 1: self-made, the preparation method comprising the following steps: taking dried Psidium guajava leaves, crushing, and passing through a 120-mesh sieve, collecting the undersize to obtain crushed Psidium guajava leaves, then adding water into the crushed Psidium guajava leaves and grinding into a slurry (the mass ratio of water to the crushed Psidium guajava leaves is 1:1), then adding cellulase and pectinase with a mass ratio of 1:1 (the total mass of the cellulase and the pectinase is 0.1% of the mass of the crushed Psidium guajava leaves), and carrying out enzymolysis at 40 °C for 4 h, after the enzymolysis, heating to 90 °C for 1 h, thereby obtaining an enzymolysis product, then adding the enzymolysis product into a 60% ethanol aqueous solution (the mass-volume ratio of the enzymolysis product to the ethanol aqueous solution is 1 g:10 mL), and carrying out extraction at 70 °C for 6 h, after the extraction is completed, filtration is carried out, the filter residue is collected and concentrated, then vacuum drying at 40 °C for 24 h, thereby Psidium guajava leaf extract 1 is obtained;

[0064] Psidium guajava leaf extract 2: self-made, the preparation method comprising the following steps: taking dried Psidium guajava leaf, crushing and passing through a 120-mesh sieve, collecting the undersize, obtaining crushed Psidium guajava leaf, then adding water to the Psidium guajava leaf and grinding into a slurry (the mass ratio of water to the crushed Psidium guajava root is 1:1), then adding cellulase and pectinase (the total mass of cellulase and pectinase is 0.5% of the mass of the crushed Psidium guajava root) with a mass ratio of 1:4, and carrying out enzymolysis at 30 ℃ for 2 h, then heating to 90 ℃ for 1 h, obtaining an enzymolysis product, then adding the enzymolysis product to 40% ethanol aqueous solution (the mass-volume ratio of the enzymolysis product to the ethanol aqueous solution is 1 g:10 mL), and carrying out extraction at 90 ℃ for 2 h, filtering after the extraction is completed, collecting the filter residue and concentrating, then placing under vacuum drying at 40 ℃ for 24 h, obtaining Psidium guajava leaf extract 2;

[0065] Psidium guajava leaf extract 3: self-made, the preparation method comprising the following steps: taking dried Psidium guajava leaf, crushing and passing through a 120-mesh sieve, collecting the undersize, obtaining crushed Psidium guajava leaf, then adding water to the Psidium guajava leaf and grinding into a slurry (the mass ratio of water to the crushed Psidium guajava root is 1:1), then adding cellulase (the mass of cellulase is 0.1% of the mass of the crushed Psidium guajava root), and carrying out enzymolysis at 30 ℃ for 2 h, heating to 90 ℃ for 1 h after the enzymolysis, obtaining an enzymolysis product, then adding the enzymolysis product to 60% ethanol aqueous solution (the mass-volume ratio of the enzymolysis product to the ethanol aqueous solution is 1 g:10 mL), and carrying out extraction at 90 ℃ for 2 h, filtering after the extraction is completed, collecting the filter residue and concentrating, then placing under vacuum drying at 40 ℃ for 24 h, obtaining Psidium guajava leaf extract 3;

[0066] Psidium guajava leaf extract 4: self-made, the preparation method comprising the following steps: taking dried Psidium guajava leaf, crushing and passing through a 120-mesh sieve, collecting the undersize, obtaining crushed Psidium guajava leaf, then adding 60% ethanol aqueous solution to the crushed Psidium guajava leaf (the mass-volume ratio of the crushed Psidium guajava leaf to the ethanol aqueous solution is 1 g:10 mL), and carrying out extraction at 90 ℃ for 4 h, filtering after the extraction is completed, collecting the filter residue and concentrating, then placing under vacuum drying at 40 ℃ for 24 h, obtaining Psidium guajava leaf extract 4.

[0067] Examples 1-8 and Comparative Examples 1-4

[0068] The examples and comparative examples of the present application provide a firming and anti-wrinkle composition, components (mass parts) of the firming and anti-wrinkle composition are shown in Tables 1-2;

[0069] Table 1

[0070]

[0071] The preparation method of the firming and anti-wrinkle composition provided in Example 1 comprises the following steps:

[0072] (1) platinum powder was added to methanol and heated to reflux for 2 hours, then cooled to room temperature and allowed to stand for 20 hours, followed by filtration to collect the residue and drying, to obtain pretreated platinum powder;

[0073] (2) the pretreated platinum powder, palmitoyl pentapeptide-4, carnosine, Walsura robusta extract, Guettarda speciosa leaf extract were mixed uniformly to obtain the firming and anti-wrinkle composition;

[0074] The preparation method of Examples 2-8 and Comparative Examples 1-4 was consistent with that of Example 1, and no related components were added.

[0075] Example 9

[0076] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. platinum powder 2 is used instead of platinum powder 1.

[0077] Example 10

[0078] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. platinum powder 3 is used instead of platinum powder 1.

[0079] Example 11

[0080] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. Walsura robusta extract 2 is used instead of Walsura robusta extract 1.

[0081] Example 12

[0082] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. Walsura robusta extract 3 is used instead of Walsura robusta extract 1.

[0083] Example 13

[0084] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. Walsura robusta extract 4 is used instead of Walsura robusta extract 1.

[0085] Example 14

[0086] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. Guettarda speciosa leaf extract 2 is used instead of Guettarda speciosa root extract 1.

[0087] Example 15

[0088] The firming and anti-wrinkle composition of the present application has only one difference from that of Example 1, i.e. Guettarda speciosa leaf extract 3 is used instead of Guettarda speciosa root extract 1.

[0089] Example 16

[0090] The inventive example provides a firming anti-wrinkle composition, the only difference of which from Example 1 is that Psidium guajava leaf extract 4 is used instead of Psidium guajava root extract 1.

[0091] Comparative Example 5

[0092] The inventive comparative example provides a firming anti-wrinkle composition, the only difference of which from Example 1 is that Aloe barbadensis leaf extract is used instead of Pentas lanceolata extract 1.

[0093] Comparative Example 6

[0094] The inventive comparative example provides a firming anti-wrinkle composition, the only difference of which from Example 1 is that Psidium guajava fruit extract is used instead of Psidium guajava leaf extract 1.

[0095] Comparative Example 7

[0096] The inventive comparative example provides a firming anti-wrinkle composition, the only difference of which from Example 1 is that Tripeptide-1 is used instead of Carosine.

[0097] Comparative Example 8

[0098] The inventive comparative example provides a firming anti-wrinkle composition, the only difference of which from Example 1 is that Acetyl Hexapeptide-8 is used instead of Palmitoyl Pentapeptide-4.

[0099] Application Examples 1-17 and Comparative Application Examples 1-9

[0100] The inventive application examples and comparative application examples provide an emulsion, the components (mass percentage) of which are shown in Table 3; wherein the firming anti-wrinkle compositions in application examples 1-16 are respectively the firming anti-wrinkle compositions prepared in Examples 1-16, the firming anti-wrinkle compositions in comparative application examples 1-8 are respectively the firming anti-wrinkle compositions in Comparative Examples 1-8, and the firming anti-wrinkle composition used in application example 17 is the firming anti-wrinkle composition prepared in Example 1.

[0101] Table 3

[0102]

[0103]

[0104] The preparation method of the emulsion provided in application example 1 comprises the following steps:

[0105] (1) Mix the humectant and thickening agent with deionized water, stir, heat to 85°C, then homogenize at a speed of 1000 rpm for 5 min, and keep warm after homogenization for standby, to obtain a pre-prepared A component;

[0106] (2) Mix the emulsifier, heat to 85°C, then homogenize at a speed of 1000 rpm for 5 min, and keep warm after homogenization for standby, to obtain a pre-prepared B component;

[0107] (3) Mix the preservative, heat to 60°C to melt, to obtain a pre-prepared C component;

[0108] (4) Warm the pre-prepared A component to 80°C, add the pre-prepared B component at a speed of 400 rpm, stir and mix, then cool to 60°C, add the pre-prepared C component at a speed of 400 rpm, stir and mix, then cool to 40°C, add the firming and anti-wrinkle composition, continue to stir for 6 min, finally add the remaining pH adjuster to adjust the pH to 6.0, stop stirring, discharge, to obtain an emulsion.

[0109] The preparation method and application of the emulsion in application examples 2-17 and comparative application examples 1-9 are consistent with application example 1, and no related components are added.

[0110] Effect example 1

[0111] The firming and anti-wrinkle compositions prepared in examples 1-16 and comparative examples 1-8 are verified for their promotion of elastin and antioxidant effects.

[0112] 1. Promotion of elastin

[0113] The test is carried out in accordance with the standard of T / ZHCA 015-2022 “Cosmetic Firming Effect Evaluation Zebrafish Larvae Elastin Gene Relative Expression Method”, and three concentration groups are set during the test;

[0114] The gene expression promotion rate is calculated according to the gene expression obtained by the test, and the gene expression promotion rate = (blank group gene expression - test group gene expression) / blank group gene expression × 100%, and the results of the Eln1 gene expression promotion rate calculated are shown in Table 4;

[0115] 2. Antioxidant effect

[0116] The test is carried out in accordance with the standard of T / SHRH 006-2018 Cosmetics - Free Radical (DPPH) Scavenging Experiment Method, and the 1h scavenging rate is recorded in Table 4;

[0117] Table 4

[0118]

[0119]

[0120] As can be seen from Table 4, when the technical solution provided by the present application is adopted, the obtained firming and anti-wrinkle composition has excellent effects of promoting expression of elastin gene and scavenging DPPH; specifically, the expression promotion rate of the obtained firming and anti-wrinkle composition on elastin gene Eln1 is above 71%, and the 1h scavenging rate on DPPH is above 82%;

[0121] As can be seen from Examples 1-8, the mass parts of the components in the firming and anti-wrinkle composition can affect the performance of the product, when the mass parts of the components in the firming and anti-wrinkle composition are further selected within the preferred range given by the present application, the comprehensive performance of the obtained product is better, specifically, the expression promotion rate of the obtained firming and anti-wrinkle composition on elastin gene Eln1 is above 80%, and the 1h scavenging rate on DPPH is above 88%;

[0122] As can be seen from Examples 1 and Comparative Examples 1-4, when one of the components in the present application is not added, the expression promotion rate of the obtained firming and anti-wrinkle composition on elastin gene and the scavenging rate on DPPH all show a significant downward trend; as can be seen from Examples 1 and Comparative Examples 5-8, when other similar components are used to replace the components in the present application, the effects in the present application cannot be achieved.

[0123] Effect Example 2

[0124] The safety of the emulsions provided by Application Examples 1-17 and Comparative Application Examples 1-9 is verified, specifically including the following steps:

[0125] 30 volunteers aged 20-50 years old are selected, the patch tester cells are numbered, and the test samples are respectively placed in the corresponding numbered patch tester cells, with a dosage of 0.02mL per cell; the patch tester with the test sample is attached to the back of the test subject with a low-sensitization adhesive tape, and the palm is lightly pressed to make it evenly attached to the skin for 24h. Then the patch tester is removed, and the skin condition of the created site is observed, and the identification standard is shown in Table 5;

[0126] Table 5

[0127] Grade Symbol Criteria 0 － Negative reaction: no irritation, no erythema 1 ± Suspect reaction: mild erythema 2 + Weak positive reaction: erythema 3 ++ Strong positive reaction: erythema, papules, vesicles 4 +++ Very strong positive reaction: severe edema, large blisters

[0128] It is found through identification that the emulsion prepared by the technical solution of the present application has small skin irritation, which meets the requirements of the Hygienic Standard for Cosmetics for non-irritation of cosmetics.

[0129] Effect Example 3

[0130] The human application effect of the emulsion provided by Application Examples 1-17 and Comparative Application Examples 1-9 is verified, specifically including the following steps:

[0131] Select 156 volunteers with obvious wrinkles on the face, aged 30-60 years old, randomly divided into 26 groups, 6 people in each group; under normal circumstances, the volunteers continuously use the products of the corresponding application examples 1-17 and comparative application example 1-9 on the whole face for 28 days, and evaluate the efficacy of the application examples and comparative application examples;

[0132] Specifically: the volunteers take an appropriate amount of emulsion after cleansing and moisturizing in the morning and evening, apply it to the face, massage and absorb it, use it on the whole face once in the morning and evening, and return on the 0th, 7th, 14th and 28th day. The volunteers visit the test area after washing their faces with water without applying any products, sit in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The improvement of facial indicators is quantified and photographed using instruments, including using Primos to measure the area of the marionette lines; using a skin transepidermal water loss probe TM Hex(Courage+Khazaka) to test TEWL value, using Colorimeter CL400 to test ITA° value; using Cutometer (MPA580, Courage and Khazaka, Germany) to test skin tightness parameter F4; then record and calculate the improvement rate at 28 days according to the following formula;

[0133] Pre-and post-improvement rate = |(X 使用前均值 -X 使用后均值 ) / X 使用前均值 × 100%; the results are shown in Table 6;

[0134] Table 6

[0135]

[0136]

[0137] As can be seen from Table 6, when the technical solution provided by the present application is used, compared with comparative application example 9 (equivalent to a blank group), the obtained emulsion has excellent tightening and anti-wrinkle, skin lightening and moisturizing effects; specifically, after being applied to the human body for 28 days, the improvement rate (specifically the reduction rate) of the marionette line area is more than 21.14%, the improvement rate of TEWL value is more than 12.66%, the improvement rate of ITA° value is more than 10.01%, and the improvement rate of F4 value is more than 11.23%;

[0138] As can be seen from application examples 1-8, the mass parts of the components in the firming and anti-wrinkle composition affect the comprehensive performance of the product, and when the mass parts of the components in the firming and anti-wrinkle composition are further selected within the preferred range of the present application, the comprehensive performance of the product is better, and specifically, after application to the human body for 28 days, the improvement rate (specifically, the reduction rate) of the area of the command wrinkle is above 26.05%, the improvement rate of the TEWL value is above 16.57%, the improvement rate of the ITA° value is above 14.24%, and the improvement rate of the F4 value is above 15.15%; as can be seen from application example 1 and application examples 9-16, the parameters of the components in the firming and anti-wrinkle composition or the preparation method of the extract also affect the comprehensive performance of the product to a certain extent.

[0139] As can be seen from application example 1 and comparative application examples 1-4, when a certain component is missing in the added firming and anti-wrinkle composition, the comprehensive effect of the obtained emulsion is obviously decreased; as can be seen from application example 1 and comparative application examples 5-8, when other similar components are used to replace the components in the firming and anti-wrinkle composition of the present application, the obtained emulsion also cannot achieve the effect in the present application.

[0140] Finally, it should be noted that the above examples are used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application, and although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The firming and anti-wrinkle composition is composed of the following components in parts by weight: 0.0005-0.002 parts of platinum powder, 1-5 parts of palmitoyl pentapeptide-4, 0.05-0.4 parts of carnosine, 0.1-0.5 parts of Balkan chicory extract, 0.3-1.4 parts of guava leaf extract; The average particle size of the platinum powder is 0.2-0.3 μm; In the firming and anti-wrinkle composition, the mass percentage of guava leaf extract is 5-50%; The preparation method of the guava leaf extract comprises the following steps: drying and crushing the guava leaves, followed by enzymatic hydrolysis, keeping the leaves warm after the enzymatic hydrolysis to obtain an enzymatic hydrolysis product, adding the enzymatic hydrolysis product to an ethanol aqueous solution for extraction, filtering the enzymatic hydrolysis product after the extraction, collecting the filtrate, and concentrating and drying the filtrate; in the enzymatic hydrolysis, the enzymes include cellulase and pectinase in a mass ratio of 1:(1-4), and the mass percentage of ethanol in the ethanol aqueous solution is 40-70%; The preparation method of the Balkan chicory extract comprises the following steps: drying the Balkan chicory, crushing it, then immersing it in an ethanol aqueous solution, ultrasonically extracting it after the immersion, filtering it after the extraction, collecting the filtrate, and concentrating and drying it to obtain the Balkan chicory extract, wherein the mass percentage of ethanol in the ethanol aqueous solution is 30-60%.

2. The firming and anti-wrinkle composition according to claim 1, characterized in that The firming and anti-wrinkle composition is composed of the following components in parts by weight: 0.001-0.0015 parts of platinum powder, 2-3 parts of palmitoyl pentapeptide-4, 0.2-0.3 parts of carnosine, 0.2-0.3 parts of Balkan chicory extract, and 0.5-0.8 parts of guava leaf extract.

3. The firming and anti-wrinkle composition according to claim 1, characterized in that In the firming and anti-wrinkle composition, the mass ratio of the sum of the mass of palmitoyl pentapeptide-4 and carnosine to the Balkan chicory extract is (10-14):

1.

4. The firming and anti-wrinkle composition according to claim 1, characterized in that The immersion temperature is 20-40°C and the immersion time is 12-24h; And / or, the ultrasonic power is 120-160W, the ultrasonic time is 10-40min, and the ultrasonic temperature is 40-55°C.

5. The firming and anti-wrinkle composition according to claim 1, characterized in that: In the enzymatic hydrolysis, the added mass of the enzyme is 0.1-0.5% of the mass of the guava fruit; And / or, in the enzymolysis, the enzymolysis time is 2-4 hours and the enzymolysis temperature is 30-45°C.

6. The firming and anti-wrinkle composition according to claim 1, characterized in that: The extraction temperature is 60-90° C., and the extraction time is 2-6 hours.

7. The method for preparing the firming and anti-wrinkle composition according to any one of claims 1 to 6, wherein: The preparation method comprises the following steps: (1) Platinum powder is added to an organic solvent and heated under reflux, then cooled to room temperature and allowed to stand, and then filtered to collect the residue and dry it to obtain pretreated platinum powder; (2) The pretreated platinum powder, palmitoyl pentapeptide-4, carnosine, Balkan chicory extract, and guava leaf extract are mixed evenly to obtain a firming and anti-wrinkle composition.

8. Use of the firming and anti-wrinkle composition according to any one of claims 1 to 6 in the preparation of cosmetics.

9. An emulsion, characterized in that The emulsion comprises the firming and anti-wrinkle composition according to any one of claims 1 to 6.

10. The emulsion according to claim 9, characterized in that The emulsion comprises the following components in percentage by weight: 1-8% of the firming and anti-wrinkle composition according to any one of claims 1 to 6, 2-40% of a cosmetic base, and the balance water.

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