Traditional Chinese medicine composition with antibacterial, anti-inflammatory, anti-oxidation and whitening effects as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of compositions, and particularly relates to a traditional Chinese medicine composition with bacteriostatic, anti-inflammatory, antioxidant and whitening effects and a preparation method and application thereof. The composition provided by the invention comprises the following raw materials in parts by weight: 1-5 parts of rhodiola rosea; 5-10 parts of licorice root; and 1-5 parts of phyllanthus emblica. The composition is obtained by crushing rhodiola rosea, liquorice and phyllanthus emblica into powder raw materials and adding the powder raw materials into an extraction solvent for extraction. Compared with a composition with a single purpose, the composition disclosed by the invention has the effects of inhibiting bacteria, resisting inflammation, resisting oxidation and whitening at the same time, and the four effects of the product can be met at the same time.

Description

Technical Field

[0001] The invention belongs to the technical field of compositions, and particularly relates to a Chinese medicine composition having antibacterial, anti-inflammatory, antioxidant and whitening effects, and a preparation method and application thereof. Background Art

[0002] With the development of society, people's lifestyles are constantly improving, their living environments are constantly changing, and people's health awareness is also constantly improving. Therefore, daily chemical products with multiple functions have become a new demand for people's daily consumption. The skin is the largest organ of the human body, and it undertakes multiple functions such as protecting the body, perspiration, sensing cold and heat, and pressure. Under normal circumstances, there are many microorganisms on the surface of human skin, which maintain a microecological balance with the human body, thereby maintaining the health of the human body. Once this balance is broken, it may cause skin inflammation, redness, swelling, itching and other problems, and even lead to more serious diseases. Therefore, how to effectively inhibit skin pathogens and maintain microecological balance at the same time is a basic requirement for skin cleaning and skin care products. On this basis, if it can have anti-inflammatory, antioxidant, whitening and other effects, it will also increase the scope of use of related products and increase the comfort of consumers.

[0003] Traditional usage of Chinese medicine and its compound is mainly oral administration, supplemented by external use, so the extraction solvent used is mostly water, and only a very small number of plaster preparations use vegetable oil as an extraction solvent. What active effects will be produced by using cosmetic base oil as a solvent to extract Chinese medicine? Due to the lack of necessary research data, relevant reports are rare. Therefore, it is necessary to provide a Chinese medicine skin care raw material using base oil as a solvent. Through a large number of experiments, the present invention has determined that using base oil as a solvent, Rhodiola rosea, Licorice, and Phyllanthus emblica in combination has significant antibacterial, anti-inflammatory, antioxidant, and whitening effects, and can be used in finished cosmetics. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a traditional Chinese medicine composition having antibacterial, anti-inflammatory, antioxidant and whitening effects. The composition has the advantages of antibacterial, anti-inflammatory, antioxidant and whitening, and makes up for the defect that the above raw materials have a single effect. In addition, the composition of the present invention uses base oil as a solvent, has strong activity and light color, and is particularly suitable for skin care products such as emulsions, creams, makeup remover oils, and essences.

[0005] The composition of the invention comprises the following raw materials in parts by weight: 1 to 5 parts of rhodiola rosea; 5 to 10 parts of liquorice; and 1 to 5 parts of emblica fruit.

[0006] The preparation method of the above-mentioned Chinese medicine composition comprises the following steps:

[0007] (1) 1 to 5 parts of Rhodiola rosea, 5 to 10 parts of Licorice, and 1 to 5 parts of Emblica officinalis are crushed and passed through a 10 to 20 mesh sieve to obtain a powder raw material;

[0008] (2) adding the powdered raw material in (1) to an extraction solvent at a mass volume ratio of 1 g to (2-20) mL, extracting at 50° C. to 180° C. for 0.5 h to 3.0 h, filtering through a 60-100 mesh filter cloth, discarding the drug residue, collecting the filtrate, and obtaining a filtrate; the extraction solvent is selected from at least one of coconut oil alcohol-caprylate / caprate, propylheptyl caprylate, phytosterol / octyldodecanol lauroyl glutamate, triolein, meadowfoam seed oil, squalane, coconut oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, rosehip oil, sesame oil, wheat germ oil, camellia seed oil, and caprylic / capric triglyceride;

[0009] (3) Filter the filtrate obtained in (2) through a 2 μm to 10 μm filter plate until the filtrate is clear, obtaining a clear filtrate and a Chinese medicine composition.

[0010] The above-mentioned traditional Chinese medicine composition is used in the preparation of antibacterial products, anti-inflammatory products, antioxidant products and whitening products.

[0011] Rhodiola rosea tastes bitter and is cold in nature. It has the effects of clearing away heat and dampness, and strengthening the body. Modern research shows that Rhodiola rosea contains rich chemical components, including flavonoids, organic acids, trace elements, etc. Its main active ingredient is salidroside, in addition to tyrosol, quercetin, etc. Pharmacological activity studies have shown that Rhodiola rosea has multiple pharmacological effects such as anti-hypoxia, anti-fatigue, anti-virus, anti-radiation, and anti-aging.

[0012] Licorice is sweet and mild in nature. It has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, relieving pain and harmonizing various medicines. It is mainly used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough and sputum, abdominal and limb cramps and pain, carbuncle and sore, and relieving drug toxicity. In addition to glycyrrhizic acid and glycyrrhizic acid glycosides, licorice also includes a variety of flavonoids such as glycyrrhizin, isoliquiritigenin, polysaccharides and other ingredients. Modern pharmacological studies have shown that licorice has multiple effects such as anti-inflammatory, antiviral, antioxidant, immunomodulatory, anti-atherosclerotic, and anti-tumor.

[0013] Phyllanthus emblica tastes sour, sweet, astringent, and cool in nature. It has the effects of promoting fluid production and quenching thirst, clearing away heat and detoxifying, moistening the lungs and relieving coughs, and nourishing and regenerating muscles. Modern research shows that Phyllanthus emblica contains a variety of chemical components, including polyphenols, organic acids, tannins, vitamin C, and trace elements. Its main active ingredients are ellagic acid, gallic acid, and vitamin C. Pharmacological studies have shown that Phyllanthus emblica has powerful antioxidant, anti-inflammatory, antiviral, anti-aging, immunomodulatory, and liver and kidney protection effects.

[0014] The present invention is based on the characteristics of "multi-component, multi-target, and multi-pathway" effects of traditional Chinese medicine compound, combined with the comprehensive needs of skin cleaning and care products, and conducts research around multiple effects such as antibacterial, anti-inflammatory, antioxidant, and whitening. Rhodiola rosea, licorice, and emblica fructus are compounded into a formula, which has a significant synergistic effect, can take into account multiple effects, and has the characteristics of high safety. It can be used as a functional additive in skin daily chemical products.

[0015] In cosmetics, base oil is an important raw material for formula preparation. It is usually extracted from natural plants or animals, and can also be obtained by synthesis. Common base oils for cosmetics include plant oils (such as jojoba oil, sweet almond oil, olive oil, etc.), animal oils (such as lanolin, squalane, beeswax, etc.), and synthetic oils (such as caprylic / capric triglyceride, isononyl isononanoate, isopropyl palmitate, etc.). The main functions of base oil in cosmetics are: moisturizing, nourishing the skin, dissolving active ingredients, promoting ingredient absorption, improving the stability of the formula, etc. Therefore, base oil is widely used in cosmetics such as emulsions, creams, essences, lipsticks, etc. As a raw material, base oil is also an important solvent that can be used to extract and dissolve other raw materials, thereby increasing the skin care effect of the product.

[0016] The beneficial effects of the present invention are:

[0017] (1) Compared with single-purpose compositions, the composition of the present application has antibacterial, anti-inflammatory, antioxidant and whitening effects, and can simultaneously meet the above four functions of the product;

[0018] (2) The average values ​​of the inhibition zones of Staphylococcus aureus, Propionibacterium acnes, and Trichophyton mentagrophytes were determined. The results showed that the average values ​​of the inhibition zones of the compositions of the present application were all greater than 18 mm, showing significant antibacterial effects.

[0019] (3) Compared with a single medicinal material, the composition of the present application has a stronger effect in inhibiting pro-inflammatory factors such as TNF-α, IL-1β, and IL-6;

[0020] (4) The product of the present application, the most preferred solution, has DPPH and ABTS free radical scavenging rates of 80.7% and 83.2%, respectively, indicating that the composition has high activity in scavenging free radicals;

[0021] (5) The samples in the present application have excellent inhibitory effects on tyrosinase, that is, they have excellent whitening effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The results of the inhibition zone test on Staphylococcus aureus in Effect Example 1;

[0023] Figure 2The results of the inhibition zone test on Propionibacterium acnes in Effect Example 1;

[0024] Figure 3 The results of the inhibition zone measurement on Trichophyton mentagrophytes in Effect Example 1 are shown. DETAILED DESCRIPTION

[0025] In order to enable those skilled in the art to better understand the present invention, the present invention is further described in conjunction with specific implementation methods.

[0026] Example 1

[0027] The embodiment of the present invention provides a traditional Chinese medicine composition with antibacterial, anti-inflammatory, antioxidant and whitening effects, which is prepared from Rhodiola rosea, Licorice and Phyllanthus emblica in a mass ratio of 2:5:2.

[0028] The preparation method is:

[0029] S1. Crush Rhodiola rosea, Licorice root and Phyllanthus emblica, and pass through a 20-mesh sieve to obtain a powder raw material;

[0030] S2, adding caprylic acid / capric acid triglyceride to the powder raw material in S1 at a mass volume ratio of 1:10, extracting at 110° C. for 1.0 h, filtering through an 80-mesh filter cloth, discarding the drug residue, collecting the filtrate, and obtaining a filtrate;

[0031] S3. Filter the filtrate obtained in S2 through a filter plate until the filtrate is clarified, wherein the pore size of the filter plate is 5 μm, and obtain a clarified filtrate, which is the Chinese medicine composition of the present invention.

[0032] Example 2

[0033] The embodiment of the present invention provides a traditional Chinese medicine composition with antibacterial, anti-inflammatory, antioxidant and whitening effects, which is prepared from rhodiola rosea, liquorice and emblica fruit in a mass ratio of 5:10:5.

[0034] The preparation method is:

[0035] S1. Crush Rhodiola rosea, Licorice root and Phyllanthus emblica, and pass through a 20-mesh sieve to obtain a powder raw material;

[0036] S2, adding the powdered raw material in S1 to coconut oil alcohol-caprylate / caprate in a mass volume ratio of 1:2, extracting at 180° C. for 0.5 h, filtering through a 100-mesh filter cloth, discarding the residue, collecting the filtrate, and obtaining a filtrate;

[0037] S3. Filter the filtrate obtained in S2 through a filter plate until the filtrate is clarified, wherein the pore size of the filter plate is 10 μm, and obtain a clarified filtrate, which is the Chinese medicine composition of the present invention.

[0038] Example 3

[0039] The embodiment of the present invention provides a traditional Chinese medicine composition with antibacterial, anti-inflammatory, antioxidant and whitening effects, which is prepared from rhodiola rosea, liquorice and emblica fruit in a mass ratio of 1:10:1.

[0040] The preparation method is:

[0041] S1. Grind rhodiola rosea, liquorice and emblica fruit, and pass through a 10-mesh sieve to obtain a powder raw material;

[0042] S2, adding olive oil to the powdered raw material in S1 at a mass volume ratio of 1:20, extracting at 50° C. for 3.0 h, filtering through an 80-mesh filter cloth, discarding the drug residue, collecting the filtrate, and obtaining a filtrate;

[0043] S3. Filter the filtrate obtained in S2 through a filter plate until the filtrate is clarified, wherein the pore size of the filter plate is 2 μm, and obtain a clarified filtrate, which is the Chinese medicine composition of the present invention.

[0044] Example 4

[0045] The embodiment of the present invention provides a traditional Chinese medicine composition with antibacterial, anti-inflammatory, antioxidant and whitening effects, which is prepared from rhodiola rosea, liquorice and emblica fruit in a mass ratio of 1:9:4.

[0046] The preparation method is:

[0047] S1. Crush Rhodiola rosea, Licorice root and Phyllanthus emblica, and pass through a 20-mesh sieve to obtain a powder raw material;

[0048] S2, adding phytosterol / octyldodecanol lauroyl glutamate to the powdered raw material in S1 at a mass volume ratio of 1:8, extracting at 100° C. for 1.5 h, filtering through a 100-mesh filter cloth, discarding the drug residue, collecting the filtrate, and obtaining a filtrate;

[0049] S3. Filter the filtrate obtained in S2 through a filter plate until the filtrate is clarified, wherein the pore size of the filter plate is 8 μm, and obtain a clarified filtrate, which is the Chinese medicine composition of the present invention.

[0050] Example 5

[0051] The embodiment of the present invention provides a traditional Chinese medicine composition with antibacterial, anti-inflammatory, antioxidant and whitening effects, which is prepared from rhodiola rosea, licorice and emblica fruit in a mass ratio of 2:8:3.

[0052] The preparation method is:

[0053] S1. Grind rhodiola rosea, liquorice and emblica fruit, and pass through a 10-mesh sieve to obtain a powder raw material;

[0054] S2, adding camellia oil to the powdered raw material in S1 at a mass volume ratio of 1:12, extracting at 160° C. for 2.5 h, filtering through an 80-mesh filter cloth, discarding the drug residue, collecting the filtrate, and obtaining a filtrate;

[0055] S3. Filter the filtrate obtained in S2 through a filter plate until the filtrate is clarified, wherein the pore size of the filter plate is 8 μm, and obtain a clarified filtrate, which is the Chinese medicine composition of the present invention.

[0056] Example 6

[0057] The embodiment of the present invention provides a traditional Chinese medicine composition with antibacterial, anti-inflammatory, antioxidant and whitening effects, which is prepared from rhodiola rosea, liquorice and emblica fruit in a mass ratio of 2:6:3.

[0058] The preparation method is:

[0059] S1. Grind rhodiola rosea, liquorice and emblica fruit, and pass through a 10-mesh sieve to obtain a powder raw material;

[0060] S2, adding sweet almond oil to the powdered raw material in S1 at a mass volume ratio of 1:16, extracting at 150° C. for 1.5 h, filtering through a 100-mesh filter cloth, discarding the residue, collecting the filtrate, and obtaining a filtrate;

[0061] S3. Filter the filtrate obtained in S2 through a filter plate until the filtrate is clarified, wherein the pore size of the filter plate is 10 μm, and obtain a clarified filtrate, which is the Chinese medicine composition of the present invention.

[0062] Comparative Example 1

[0063] This comparative example provides a product made from a single prescription of Rhodiola rosea. The amount of the single prescription of Rhodiola rosea used in comparative example 1 is the sum of the amounts of Rhodiola rosea, licorice, and emblica fruit used in example 3, and its preparation method is the same as that of example 3.

[0064] Comparative Example 2

[0065] This comparative example provides a product made from a single liquorice prescription. The amount of the single liquorice prescription used in comparative example 2 is the sum of the amounts of Rhodiola rosea, liquorice, and emblica fruit used in example 3, and its preparation method is the same as that in example 3.

[0066] Comparative Example 3

[0067] This comparative example provides a product made from a single recipe of Phyllanthus emblica. The amount of the single recipe of Phyllanthus emblica taken in comparative example 3 is the sum of the amounts of Rhodiola rosea, Licorice and Phyllanthus emblica in Example 3, and its preparation method is the same as that in Example 3.

[0068] Comparative Example 4

[0069] This comparative example provides a product made of a Chinese medicine composition consisting of Rhodiola rosea and licorice, which is prepared from Rhodiola rosea and licorice in a mass ratio of 1:10. The amount of Rhodiola rosea and licorice taken in Comparative Example 4 is the sum of the amounts of Rhodiola rosea, licorice, and Phyllanthus emblica in Example 3, and its preparation method is the same as Example 3.

[0070] Comparative Example 5

[0071] This comparative example provides a product made of a Chinese medicine composition consisting of licorice and emblica, which is prepared from licorice and emblica in a mass ratio of 10:1. The amount of licorice and emblica taken in comparative example 5 is the sum of the amounts of rhodiola rosea, licorice, and emblica in Example 3, and its preparation method is the same as that in Example 3.

[0072] Comparative Example 6

[0073] This comparative example provides a product made of a Chinese medicine composition consisting of Rhodiola rosea and Phyllanthus emblica, which is prepared from Rhodiola rosea and Phyllanthus emblica in a mass ratio of 1:1. The amount of Rhodiola rosea and Phyllanthus emblica taken in Comparative Example 6 is the sum of the amounts of Rhodiola rosea, Licorice, and Phyllanthus emblica in Example 3, and its preparation method is the same as Example 3.

[0074] Comparative Example 7

[0075] This comparative example provides a product made of a Chinese medicine composition consisting of Rhodiola rosea, Licorice, and Phyllanthus emblica, which is prepared from Rhodiola rosea, Licorice, and Phyllanthus emblica in a mass ratio of 1:10:1. The preparation method is basically the same as that in Example 1, except that deionized water is used instead of caprylic / capric triglyceride when extracting the raw materials.

[0076] Comparative Example 8

[0077] This comparative example provides a product made of a Chinese medicine composition consisting of Rhodiola rosea, Licorice and Phyllanthus emblica, which is prepared from Rhodiola rosea, Licorice and Phyllanthus emblica in a mass ratio of 1:10:1. The preparation method is basically the same as that in Example 1, except that 70% ethanol is used instead of caprylic / capric triglyceride when extracting the raw materials.

[0078] Experimental effect verification

[0079] Effect Example 1

[0080] This effect example provides the antibacterial effect of the traditional Chinese medicine composition prepared in Examples 1 to 6 and Comparative Examples 1 to 8 of the present invention.

[0081] 1. Experimental methods

[0082] The diameter of the inhibition zone of each sample was determined by the agar punching method. On the clean bench, the sterilized tryptic soy agar medium (TSA) was cooled to about 60°C, 20 mL of the culture medium was poured into the culture dish, and it was left to stand horizontally until it was completely solidified. Then an Oxford cup (6 mm inner diameter, 10 mm high) was placed on the surface of the agar culture medium, and a hole was punched with gentle pressure. The culture medium in the agar hole was picked out with a sterile needle, and 0.1 mL of the test bacterial suspension was taken on the plate, and the bacterial solution was evenly spread with a spreader to make a bacterial plate. Then the prepared samples (80 μL per well) were injected in sequence. Then the culture dish was placed in a 37°C constant temperature incubator for 24 hours, and the diameter of the inhibition zone was measured with a vernier caliper, and a 0.9% sterile sodium chloride solution was used as a blank control group.

[0083] 2. Experimental results

[0084] The results of inhibition zone determination are shown in Table 1 and Figure 1 to Figure 3 .

[0085] Table 1 Inhibition zone determination results (n=3, )

[0086]

[0087]

[0088] Note: Compared with Comparative Example 1, # P<0.05, ## P<0.01. Compared with Comparative Example 2, * P<0.05, ** P<0.01. Compared with comparative example 3, $ P<0.05, $$ P<0.01. Compared with Comparative Example 4, & P<0.05, && P<0.01. Compared with comparative sample 5, Compared with comparative sample 6, § P<0.05, §§ P<0.01. Compared with comparative sample 7, ￥ P<0.05, ￥￥ P<0.01. Compared with Comparative Example 8, α P<0.05, αα P<0.01.

[0089] According to the results of Table 1, compared with Comparative Examples 1 to 8, the compositions of Examples 1 to 6 showed significant antibacterial effects on Staphylococcus aureus, Propionibacterium acnes, and Trichophyton mentagrophytes (P<0.01), while the antibacterial effect of Comparative Example 1 was poor, and there was no antibacterial effect on the three pathogenic bacteria. Among them, the antibacterial effect of Example 3 was particularly outstanding, significantly better than other examples and comparative samples. Each embodiment has the best inhibitory effect on Propionibacterium acnes. And the embodiments showed significantly better effects than the single prescription of the comparative example and the combination of the two comparison examples in the antibacterial circle diameter of the antibacterial experiment. The specific ratio of Rhodiola rosea, Licorice and Phyllanthus emblica significantly improved the overall antibacterial efficacy of the Chinese medicine composition, which is better than the single application of each medicinal material and its two-to-two combination. The experimental results show that the compatibility in the embodiment can effectively enhance the synergistic effect between the medicinal materials, thereby showing a significant advantage in antibacterial performance. In addition, the comparison results of Comparative Examples 7 and 8 with Example 3 show that although the formula ratios of the three are the same, the difference in the extraction solvent leads to significantly different antibacterial effects. The oil solvent can better dissolve the fat-soluble active ingredients in the medicinal materials during the extraction process, such as certain flavonoid compounds, which may play an important role in the antibacterial mechanism. Therefore, the oil solvent extraction system of the Chinese medicine composition has potential application value as an antibacterial raw material in the field of skin care, especially for inhibiting pathogenic bacteria associated with skin infections.

[0090] Effect Example 2

[0091] This effect example provides the anti-inflammatory effect of the Chinese medicine composition prepared in Examples 1 to 6 and Comparative Examples 1 to 8 of the present invention.

[0092] 1. Experimental methods

[0093] Mouse mononuclear macrophage RAW264.7 was purchased from Wuhan Pronocell Life Science Co., Ltd. RAW264.7 cells were inoculated in DMEM medium containing 10% FBS and 1% penicillin / streptomycin. RAW264.7 cells were inoculated in the anti-inflammatory test. 5 / well) were inoculated into a 24-well plate, and a blank control group, an LPS group, and each drug group and a positive drug group (dexamethasone) were set up. After culturing in a 5% CO2, 37°C constant temperature incubator for 12 hours, the samples of each embodiment of the present invention (20 μg / mL), each comparative sample (20 μg / mL), and a positive drug (dexamethasone, 20 μg / mL) were pre-administered for protection for 12 hours, and then lipopolysaccharide (LPS, 1 μg / mL) was used to induce RAW264.7 cells for 24 hours. After the induction, the cell culture supernatant was collected, and the generation of inflammatory factors was detected using an ELISA kit.

[0094] 2. Statistical methods

[0095] The experimental data were statistically analyzed using SPSS 22.0 statistical software, and the statistical value was expressed as mean ± standard deviation. The comparison among groups was performed by one-way analysis of variance.

[0096] 3. Experimental results

[0097] The results of the proinflammatory cytokines TNF-α, IL-1β, and IL-6 are shown in Table 2.

[0098] Table 2 Inflammation index results (n=3, )

[0099]

[0100] Note: Compared with the blank group, Compared with the model group, Compared with Comparative Example 1, # P<0.05, ## P<0.01. Compared with Comparative Example 2, * P<0.05, ** P<0.01. Compared with comparative example 3, $ P<0.05, $$ P<0.01. Compared with Comparative Example 4, & P<0.05, && P<0.01. Compared with comparative sample 5, Compared with comparative sample 6, § P<0.05, §§ P<0.01. Compared with comparative sample 7, ￥ P<0.05, ￥￥ P<0.01. Compared with Comparative Example 8, α P<0.05, αα P<0.01.

[0101] According to the results in Table 2, LPS stimulation significantly induced the expression of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 in RAW264.7 cells, and the level of inflammatory factors in the model group was significantly increased (P<0.01), confirming that LPS successfully established an inflammatory model. The Chinese medicine compositions of Examples 1-6 all significantly inhibited the expression of these inflammatory factors (P<0.01), showing significant anti-inflammatory effects, especially Example 3, which was most prominent in reducing TNF-α, IL-1β, and IL-6, which may be related to the unique ratio of medicinal materials and synergistic effects.

[0102] Compared with Comparative Examples 1 to 8, the example group showed a more significant effect in inhibiting inflammatory factors (P<0.01), which shows that the ratio and interaction of the medicinal materials in the present invention greatly enhance the anti-inflammatory activity of the composition, which is better than a single medicinal material or a combination of two medicinal materials. In particular, the direct comparison of Comparative Example 7 (water extraction), Comparative Example 8 (70% ethanol extraction) and Example 3 (oil solvent extraction) further highlights the effect of the extraction solvent on the anti-inflammatory properties. Although the formulas of Comparative Examples 7 and 8 are the same as those of Example 3, since Comparative Examples 7 and 8 use water and 70% ethanol as extraction solvents, their effects in inhibiting pro-inflammatory factors such as TNF-α, IL-1β, and IL-6 are significantly weaker than those of Example 3. These results provide a strong scientific basis for the development of highly effective anti-inflammatory skin products and have broad application potential.

[0103] Effect Example 3

[0104] This effect example provides the antioxidant effects of the traditional Chinese medicine compositions prepared in Examples 1 to 6 and Comparative Examples 1 to 8 of the present invention.

[0105] 1. Experimental methods

[0106] 1.1 Effect on DPPH free radical scavenging

[0107] Taking the sample prepared in Example 1 as an example, the DPPH free radical scavenging rate was tested as follows:

[0108] The control group was set up as follows: 3 mL of DPPH solution was thoroughly mixed with 1 mL of pure water to obtain a mixed solution, which was allowed to stand for 30 min. 200 μL of the mixed solution was transferred with a pipette and added to a 96-well plate. This was repeated for 3 replicates. The absorbance at 517 nm was then determined with an ELISA reader, and the average value was taken as A1.

[0109] The experimental group was set up as follows: 3 mL of DPPH solution and 1 mL of the sample prepared in Example 1 were thoroughly mixed to obtain a mixed solution, which was allowed to stand for 30 min. 200 μL of the mixed solution was transferred with a pipette and added to a 96-well plate. This was repeated for 3 replicates. The absorbance at 517 nm was then measured with an ELISA reader, and the average value was taken as A2.

[0110] The blank group was set as follows: no sample was placed in the 96-well plate, and its absorbance at 517 nm was directly detected, which was

[0111] DPPH clearance rate = ((A1-A0)-(A2-A0)) / (A1-A0)×100%.

[0112] The samples corresponding to Examples 2 to 8 and the samples corresponding to Comparative Examples 1 and 2 were used to replace the sample prepared in Example 1, and the absorbance values ​​were measured and the DPPH free radical scavenging rates were calculated.

[0113] 1.2 About ABTS + Effects of free radical scavenging

[0114] Accurately measure 1 mL of 7.4 mmol / L ABTS solution and 2.6 mmol / L K2S2O8 solution, mix and shake, store at 4°C in the dark for 12-16 hours to obtain ABTS stock solution, and then mix with distilled water in a certain proportion (so that the absorbance value A = 0.700 ± 0.02) to obtain ABTS working solution. Add 10 μL of different concentrations of the sample and 200 μL of ABTS working solution to a 96-well plate, start timing, react at room temperature in the dark for 6 minutes, and measure the absorbance at 734 nm as A1.

[0115] The calculation formula is as follows:

[0116]

[0117] A0: Replace the sample with the same volume of distilled water and follow the same procedure as A1.

[0118] 2. Experimental results

[0119] 2.1 Analysis of in vitro antioxidant assay results

[0120] Table 3 Determination results of in vitro antioxidant activity

[0121]

[0122] According to the results in Table 3, the Chinese medicine compositions obtained in Examples 1 to 6 all exhibited excellent DPPH and ABTS free radical scavenging abilities, clearly indicating that these compositions possessed significant antioxidant activity. In general, the antioxidant effects of the Examples were generally better than those of Comparative Examples 1 to 8, further verifying the significant advantages of the Chinese medicine compositions in terms of antioxidant properties.

[0123] Specifically, the performance of Example 3 was particularly outstanding, with DPPH and ABTS free radical scavenging rates reaching 80.7% and 83.2% respectively, indicating that the composition has high activity in scavenging free radicals. This may be closely related to the synergistic effect brought about by its specific medicinal material ratio, which enables the antioxidant components to fully exert their effects.

[0124] In contrast, the antioxidant effects of Comparative Examples 1 to 8 are relatively weak, and their DPPH and ABTS removal rates are all lower than 60%, indicating that the antioxidant activity of these compositions is not as good as that of the examples, which may be due to the insufficient optimization of the medicinal material ratio, and the failure to fully exert the potential of the antioxidant components. In particular, the comparison of Comparative Example 7 (using water extraction), Comparative Example 8 (using 70% ethanol extraction) and Example 3 (using oil solvent extraction) further highlights the influence of the extraction solvent on the antioxidant performance. Although the medicinal material formulas of Comparative Examples 7, Comparative Example 8 and Example 3 are exactly the same, the scavenging ability of Comparative Examples 7 and Comparative Example 8 for DPPH and ABTS is significantly weaker than that of Example 3.

[0125] In summary, the Chinese medicine compositions of Examples 1 to 6 exhibited significant antioxidant capacity, especially Examples 2 and 3, which performed best in the DPPH and ABTS free radical scavenging experiments.

[0126] Effect Example 4

[0127] This effect example provides the whitening effect (tyrosinase inhibition effect evaluation) of the traditional Chinese medicine composition prepared in Examples 1 to 6 and Comparative Examples 1 to 8 of the present invention.

[0128] 1. Experimental methods

[0129] (1) Preparation of phosphate buffer: Accurately weigh 26.797 g of Na2HPO4·12H2O and 13.803 g of NaH2PO4·2H2O, dissolve them in deionized water, and dilute to 500 mL in a volumetric flask. Add 100 mL of sodium dihydrogen phosphate solution and 100 mL of sodium hydrogen phosphate solution to a 500 mL volumetric flask, dilute to volume with deionized water, and shake well to obtain a phosphate buffer with a pH of 6.8.

[0130] (2) Preparation of sample solutions: The samples of each embodiment and comparative example were prepared into sample solutions of corresponding concentrations using DMSO.

[0131] (3) Preparation of substrate L-tyrosine solution: The substrate L-tyrosine was prepared into an L-tyrosine solution with a mass concentration of 0.05% using phosphate buffer.

[0132] (4) Preparation of tyrosinase solution: Tyrosinase was prepared into a 100 U / mL solution using phosphate buffer solution.

[0133] (5) Determination of tyrosinase inhibition rate: According to Table 4, accurately aspirate the four reaction sample solutions C1, C2, T1, and T2 except the tyrosinase solution. After keeping the temperature in a 37°C water bath for 10 minutes, add the tyrosinase solution and react for 10 minutes. Using C2 as the reference of C1, measure the absorbance A1 of the system at 475nm. Using T2 as the reference of T1, measure the absorbance A2 of the system. To account for the systematic error caused by parallel samples, 1mL of DMSO was added to C1 and C2 respectively. To eliminate the absorbance error of the sample at 475nm, 1mL of sample solution was also added to T2. Calculate the inhibition rate of tyrosinase according to the following formula, and calculate the IC50 results of each sample.

[0134] The results are shown in Table 5.

[0135]

[0136] Where A1 is the change in absorbance of the reaction solution without adding the sample, and A2 is the change in absorbance of the reaction solution after adding the sample.

[0137] Table 4 Tyrosinase Activity Inhibition Test Reagent Dosage Table

[0138]

[0139] Table 5 Results of tyrosinase activity inhibition rate (IC50, n=3, )

[0140]

[0141] Note: Compared with Comparative Example 1, # P<0.05, ## P<0.01. Compared with Comparative Example 2, * P<0.05, ** P<0.01. Compared with comparative example 3, $ P<0.05, $$ P<0.01. Compared with Comparative Example 4, & P<0.05, && P<0.01. Compared with comparative sample 5, Compared with comparative sample 6, § P<0.05, §§ P<0.01. Compared with comparative sample 7, ￥ P<0.05, ￥￥ P<0.01. Compared with Comparative Example 8, α P<0.05, αα P<0.01.

[0142] According to the data in Table 5, the samples of Examples 1 to 6 have significantly better inhibitory effects on tyrosinase than the samples of Comparative Examples 1 to 8 (P<0.01). Compared with the comparative samples, the samples of the examples show stronger tyrosinase inhibitory activity, which is reflected in the smaller IC50 value of tyrosinase inhibition. This shows that the samples of the examples have a more excellent tyrosinase inhibitory effect. Therefore, the samples of the examples can be added as cosmetic raw materials to effectively enhance the whitening effect of the final product.

[0143] The samples in the Example group have a more significant inhibitory effect on tyrosinase activity, indicating that the raw material ratio and interaction in the present invention greatly enhance the whitening activity of the composition, which is better than a single medicinal material or a combination of two medicinal materials. In particular, the direct comparison of Comparative Example 7 (water extraction) Comparative Example 8 (70% ethanol extraction) and Example 3 (oil solvent extraction) further highlights the whitening effect of the combined oil-soluble components.

Claims

1. A Chinese medicine composition having antibacterial, anti-inflammatory, antioxidant and whitening effects, comprising the following raw materials in parts by weight: 1 to 5 parts of Rhodiola rosea; 5 to 10 parts of Licorice; and 1 to 5 parts of Emblica officinalis.

2. The composition according to claim 1, characterized in that Crush Rhodiola rosea, Licorice root and Emblica officinalis into powder raw materials, add them into extraction solvent for extraction, and obtain a Chinese medicine composition; The extraction solvent is selected from at least one of coconut oil alcohol-caprylate / caprate, propylheptyl caprylate, phytosterol / octyldodecyl lauroyl glutamate, triolein, white meadowfoam seed oil, squalane, coconut oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, rosehip oil, sesame oil, wheat germ oil, camellia seed oil, and caprylic / capric triglyceride; The mass volume ratio of powdered raw material to extraction solvent is 1g: (2~20)mL.

3. The composition according to claim 2, characterized in that The mass ratio of Rhodiola rosea, Glycyrrhiza uralensis and Phyllanthus emblica is 2:5:2, and the extraction solvent is caprylic triglyceride or capric triglyceride; Or the mass ratio of Rhodiola rosea, Licorice root and Emblica officinalis is 5:10:5, and the extraction solvent is coconut oil alcohol-caprylate or coconut oil alcohol-caprate; Or the mass ratio of Rhodiola rosea, Licorice root and Emblica officinalis is 1:10:1, and the extraction solvent is olive oil; or the mass ratio of Rhodiola rosea, Licorice root and Emblica officinalis is 1:9:4, and the extraction solvent is phytosterol or octyldodecanol lauroyl glutamate; Or the mass ratio of Rhodiola rosea, Licorice root and Emblica officinalis is 2:8:3, and the extraction solvent is camellia oil; Or the mass ratios of Rhodiola rosea, Licorice and Emblica officinalis are 2:6:3 respectively, and the extraction solvent is sweet almond oil.

4. The composition according to claim 2, characterized in that Place the powdered raw material in an extraction solvent and extract at 50°C~180°C for 0.5h~3.0h.

5. The method for preparing the composition according to claim 2, comprising the following steps: (1) Crush 1-5 parts of Rhodiola rosea, 5-10 parts of Licorice, and 1-5 parts of Emblica officinalis, and pass through a 10-20 mesh sieve to obtain a powder raw material; (2) Add the powdered raw material in (1) into the extraction solvent at a mass volume ratio of 1 g: (2-20) mL, extract at 50°C-180°C for 0.5 h-3.0 h, filter through a 60-100 mesh filter cloth, discard the residue, collect the filtrate, and obtain a filtrate; (3) Filtering the filtrate obtained in (2) through a 2 μm-10 μm filter plate until the filtrate is clear, obtaining a clear filtrate and obtaining a Chinese medicine composition.

6. Use of the composition as claimed in claim 1 in the preparation of antibacterial products.

7. Use of the composition as claimed in claim 1 in the preparation of anti-inflammatory products.

8. Use of the composition as claimed in claim 1 in the preparation of antioxidant products.

9. Use of the composition as claimed in claim 1 in preparing whitening products.

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