Application of residual barley oropharynx combined traditional Chinese medicine extract in preparation of medicine for treating stomatitis

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to application of a residual barley oropharynx combined traditional Chinese medicine extract in preparation of a medicine for treating stomatitis. The invention provides application of a herba houttuyniae extract, a radix astragali extract and a fructus forsythiae extract in preparation of a medicine for treating stomatitis, wherein the stomatitis is herpangina. The herba houttuyniae extract, the radix astragali extract and the fructus forsythiae extract are combined with the herba houttuyniae extract, the radix astragali extract and the fructus forsythiae extract. The herba houttuyniae and radix astragali seu hedysari oral pharynx mixture is combined with the herba houttuyniae extract, the radix astragali seu hedysari extract and the fructus forsythiae extract, so that the synergistic interaction effect of the medicines can be fully exerted, the overall regulation advantage of the combined medicines is exerted, and the herpangina of children is effectively treated.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to use of an oropharyngeal extract of yumai combined with a traditional Chinese medicine in preparing a medicine for treating stomatitis. Background Art

[0002] Yumai oropharyngeal mixture is composed of emblica fructus, rehmannia root, red peony root, ophiopogon japonicus and liquorice. It has the effect of nourishing yin and reducing fire. It is often used to treat burning, painful, local redness and swelling, irritability, dry mouth, yellow and red urine, and recurrent oral ulcers caused by yin deficiency and excessive fire, and virtual fire ascending.

[0003] Herpetic pharyngitis is an acute infectious pharyngitis caused by enterovirus, characterized by acute fever and herpetic ulcers in the pharyngeal isthmus. It is mainly transmitted through the fecal-oral or respiratory tract, is highly contagious, and spreads quickly, either sporadically or in epidemics. The highest incidence season is summer and autumn, and it mainly affects children aged 1 to 7 years. The main clinical manifestations are fever, sore throat, small herpes on the mucosal pharyngeal isthmus, and superficial ulcers.

[0004] Patients with herpetic pharyngitis should pay attention to isolation treatment. There is currently no specific treatment for enterovirus infection. It is mainly symptomatic treatment, and attention should be paid to oral hygiene and oral hygiene. You can rinse your mouth with light salt water, apply 10% silver nitrate to the ulcer, or use Yanhou Lingdan, Bingpengsan, etc. to blow the pharynx to relieve the symptoms of pharyngitis. The present invention aims to provide an effective combination drug for the treatment of herpetic pharyngitis. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide the use of Yumai oral and pharyngeal mixture combined with a mixed extract of traditional Chinese medicine in the preparation of a drug for treating stomatitis. In particular, the combined drug of the present invention has a specific good effect in treating herpetic pharyngitis.

[0006] The invention provides use of Yumai oropharyngeal mixture combined with a mixed extract of traditional Chinese medicine in preparing a medicine for treating stomatitis.

[0007] Furthermore, the Chinese medicine mixed extract is obtained by mixing Houttuynia cordata extract, Astragalus membranaceus extract and Forsythia suspensa extract.

[0008] Furthermore, the weight proportions of the extracts in the mixed Chinese herbal extract are as follows: 6 to 12 weight proportions of Houttuynia cordata extract, 3 to 8 weight proportions of Astragalus membranaceus extract, and 1 to 6 weight proportions of Forsythia suspensa extract.

[0009] Houttuynia cordata is the fresh whole herb or dried aerial part of Houttuynia cordata Thunb. of the Saururaceae family. It tastes pungent and slightly cold. It enters the lung meridian. It clears away heat and detoxifies, eliminates carbuncle and discharges pus, and promotes diuresis and relieves stranguria. It is used for lung carbuncle vomiting pus, phlegm-heat cough, heat dysentery, heat stranguria, carbuncle, sore and ulcer. Houttuynia cordata contains chemical components such as volatile oils, flavonoids, alkaloids, phenylpropanoids, steroids, etc., and has pharmacological effects such as anti-inflammatory, anti-tumor, antibacterial and antiviral.

[0010] Astragalus is the dried root of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. of the Leguminosae family. The main active ingredients of Astragalus include flavonoids, saponins, polysaccharides and amino acids. Astragalus tastes sweet and is slightly warm. It enters the lung and spleen meridians. It replenishes qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes the production of body fluids and nourishes blood, relieves stagnation and relieves numbness, removes toxins and discharges pus, and heals sores and promotes tissue regeneration. It is used for qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, chronic diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, qi deficiency edema, internal heat and thirst, blood deficiency and sallow complexion, hemiplegia, pain and numbness in the arthralgia, carbuncle that is difficult to heal, and carbuncle that does not heal for a long time.

[0011] Forsythia suspensa (Thunb.) Vahl is a dried fruit of the Oleaceae plant Forsythia suspensa (Thunb.) Vahl. It tastes bitter and slightly cold. It enters the lung, heart and small intestine meridians. It clears away heat and detoxifies, reduces swelling and resolves stagnation, and evacuates wind-heat. It is used for carbuncle, scrofula, breast carbuncle, erysipelas, wind-heat cold, the beginning of febrile disease, warm and hot entering the camp, high fever and thirst, coma and spots, and hot stranguria and astringent pain. Forsythia suspensa mainly contains chemical components such as phenylethanol glycosides, lignans, terpenes, volatile oils, flavonoids, etc., and has multiple pharmacological effects such as anti-inflammatory, antibacterial, antiviral, and anticancer.

[0012] Furthermore, the Yumai oropharyngeal mixture is used in combination with a mixed extract of traditional Chinese medicine, and the mass ratio of the mixed extract of traditional Chinese medicine to the Yumai oropharyngeal mixture is 0.5 to 1.5:1.

[0013] Emblica emblica L. is the dried mature fruit of the Euphorbiaceae plant Phyllanthus emblica L. It tastes sweet, sour, astringent and cool. It enters the lung and stomach meridians. It has the effects of clearing heat and cooling blood, promoting digestion and strengthening the stomach, promoting body fluid and relieving cough. It is used for blood heat and blood stasis, indigestion, abdominal distension, cough, sore throat, dry mouth, etc.

[0014] Rehmannia glutinosa is the fresh or dried root of Rehmannia glutinosa Libosch., a plant of the Scrophulariaceae family. It tastes sweet and enters the heart, liver, and kidney meridians. Fresh Rehmannia glutinosa clears heat and produces body fluid, cools blood, and stops bleeding. It is used for heat disease damaging yin, red tongue and thirst, fever caused by warm toxins, vomiting blood, epistaxis, and sore throat. Raw Rehmannia glutinosa clears heat and cools blood, nourishes yin and produces body fluid. It is used for heat entering the blood, fever caused by warm toxins, vomiting blood and epistaxis, heat disease damaging yin, red tongue and thirst, body fluid damage constipation, yin deficiency fever, bone steaming and fatigue fever, and internal heat and thirst.

[0015] Ophiopogon japonicus is the dried root of Ophiopogon japonicus (Lf) Ker-Gawl.[1] of the Liliaceae family. It tastes sweet, slightly bitter, and slightly cold. It enters the heart, lung, and stomach meridians. It nourishes yin and produces body fluid, moistens the lungs and clears the heart. It is used for dry cough due to lung dryness, tuberculosis due to yin deficiency, sore throat due to throat numbness, thirst due to loss of body fluid, internal heat and thirst, restlessness and insomnia, and constipation due to dry intestines.

[0016] Red peony root is the dried root of Paeonia lactiflora Pall. or Paeonia veitchiiLynch, Ranunculaceae. It tastes bitter and slightly cold. It enters the liver meridian. It clears away heat and cools blood, dissipates blood stasis and relieves pain. It is used for heat entering the blood, fever toxicity causing rash, vomiting blood and epistaxis, red and swollen eyes, liver depression and flank pain, amenorrhea and dysmenorrhea, abdominal pain due to lumps and masses, injuries from falls, carbuncles and ulcers.

[0017] Licorice is the dried root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the Leguminosae family. It tastes sweet and is neutral. It enters the heart, lung, spleen and stomach meridians. It nourishes the spleen and replenishes qi, clears away heat and detoxifies, removes phlegm and relieves cough, relieves urgency and relieves pain, and harmonizes various medicines.

[0018] Yumai oropharyngeal mixture is commonly used to treat burning, pain, local redness and swelling, irritability, dry mouth, yellow and red urine, and recurrent oral ulcers caused by yin deficiency and hyperactivity of fire and virtual fire. The inventor creatively discovered that the combination of Yumai oropharyngeal mixture with Houttuynia cordata extract, Astragalus extract, and Forsythia suspensa extract can effectively treat herpetic pharyngitis.

[0019] Furthermore, the preparation method of the houttuynia cordata extract is: drying and crushing the houttuynia cordata, placing it in an ethanol solution for ultrasonic extraction, separating the solid and liquid to obtain a filtrate, concentrating the filtrate into an extract, and spray drying it to obtain the extract.

[0020] Furthermore, the preparation method of the houttuynia cordata extract is as follows: drying the houttuynia cordata, crushing it, placing it in an ethanol solution with a volume percentage of 50% to 70% with a mass of 3 to 6 times, and subjecting it to ultrasonic treatment twice, wherein the power of the first ultrasonic treatment is 180 to 200 W; the ultrasonic temperature is 55 to 60° C.; the ultrasonic frequency is 60 to 65 KHz, and the ultrasonic time is 0.5 to 1 h; after ultrasonic treatment, the second ultrasonic treatment is conducted after standing for 3 hours, wherein the power of the second ultrasonic treatment is 130 to 150 W; the ultrasonic temperature is 55 to 60° C.; the ultrasonic frequency is 55 to 60 KHz, and the ultrasonic time is 0.5 to 1 h. After the ultrasonic treatment, solid-liquid separation is performed to obtain a filtrate, and the filtrate is concentrated into an extract, and spray drying is performed to obtain the extract.

[0021] Furthermore, the preparation method of the astragalus extract is: taking astragalus medicinal materials, adding water to boil, combining the extracts, concentrating to extracts, and spray drying to obtain the extracts.

[0022] Furthermore, the preparation method of the astragalus extract is: take the astragalus medicinal material, wash, dry, crush, add 5 to 10 times the amount of water, heat to boiling and extract 2 to 3 times, each time for 2 to 3 hours, combine the extracts, concentrate to obtain an extract with a specific gravity of 1.10 to 1.20, and spray dry.

[0023] Furthermore, the preparation method of the Forsythia suspensa extract is: crush the Forsythia suspensa medicinal material, soak it in water, decoct it, and filter it to obtain an initial extract; add ethanol to the initial extract, perform reduced pressure distillation to obtain a concentrated solution, and spray dry it.

[0024] Furthermore, the preparation method of the Forsythia suspensa extract is as follows: take Forsythia suspensa medicinal material, wash, dry, crush, soak in 6 to 8 times the amount of water for 1 to 2 hours, heat to boiling, decoct for 2 to 3 hours, filter to obtain an initial extract; add 5 to 7 times the amount of 50 to 70% ethanol solution by volume to the initial extract, distill under reduced pressure to obtain a concentrated solution, and spray dry to obtain the concentrate.

[0025] Furthermore, the stomatitis is herpetic pharyngitis, oral ulcer, pharyngitis, periodontitis, gingivitis.

[0026] Preferably, the stomatitis is herpetic pharyngitis.

[0027] The beneficial effects of the present invention are:

[0028] The present invention combines Yumai oropharyngeal mixture with mixed extracts of traditional Chinese medicines, and the mixed extracts of traditional Chinese medicines include houttuynia extract, astragalus extract and forsythia extract. The combination of the above drugs can give full play to the synergistic effect between the drugs, give play to the overall regulatory advantages of the combined drugs, and effectively treat herpetic pharyngitis in children. In vitro tests have shown that the present invention combines Yumai oropharyngeal mixture with houttuynia extract, astragalus extract and forsythia extract to have significant effects in resisting CVA2 and CVB2 viruses. Clinical trials have shown that the Yumai oropharyngeal mixture provided by the present invention is combined with houttuynia extract, astragalus extract and forsythia extract to treat herpetic pharyngitis in children, which can effectively reduce the body temperature of the children to a normal range, so that there is no herpes in the pharyngeal isthmus, and the symptoms such as pharyngeal pain, congestion and drooling disappear, and have obvious therapeutic effects. DETAILED DESCRIPTION

[0029] The present invention will be further described in detail below through specific examples.

[0030] Example 1 Preparation of Chinese herbal medicine mixed extract

[0031] The preparation method of houttuynia cordata extract is as follows: the houttuynia cordata is dried and crushed, and placed in a 6-fold ethanol solution with a volume percentage of 70% for two ultrasonic treatments, the power of the first ultrasonic treatment is 200W, the ultrasonic temperature is 60°C, the ultrasonic frequency is 65KHz, and the ultrasonic time is 1h. After the ultrasonic treatment, the second ultrasonic treatment is performed after standing for 3h, the power of the second ultrasonic treatment is 150W, the ultrasonic temperature is 60°C, the ultrasonic frequency is 55KHz, and the ultrasonic time is 0.5h. After the ultrasonic treatment, the solid-liquid separation is performed to obtain a filtrate, and the filtrate is concentrated into an extract, and spray-dried to obtain the extract.

[0032] Preparation method of astragalus extract: take astragalus medicinal material, wash, dry, crush, add 8 times amount of water, heat to boiling and extract 3 times, each time for 2 hours, combine the extracts, concentrate to obtain an extract with a specific gravity of 1.10, and spray dry.

[0033] The preparation method of the Forsythia suspensa extract is as follows: taking Forsythia suspensa medicinal material, washing, drying, crushing, placing in 8 times the amount of water and soaking for 1.5 hours, heating to boiling, decocting for 2 hours, filtering to obtain an initial extract; adding 7 times the amount of 70% ethanol solution by volume to the initial extract, distilling under reduced pressure to obtain a concentrated solution, and spray drying to obtain the concentrate.

[0034] 12 parts by weight of Houttuynia cordata extract, 8 parts by weight of Astragalus membranaceus extract and 6 parts by weight of Forsythia suspensa extract were mixed to obtain a Chinese medicine mixed extract.

[0035] Example 2 Preparation of Chinese herbal medicine mixed extract

[0036] The preparation method of houttuynia cordata extract is as follows: the houttuynia cordata is dried and crushed, and placed in a 4-fold ethanol solution with a volume percentage of 60% for two ultrasonic treatments, the power of the first ultrasonic treatment is 180W, the ultrasonic temperature is 56°C, the ultrasonic frequency is 62KHz, and the ultrasonic time is 0.8h. After the ultrasonic treatment, the second ultrasonic treatment is performed after standing for 3h, the power of the second ultrasonic treatment is 140W, the ultrasonic temperature is 56°C, the ultrasonic frequency is 55KHz, and the ultrasonic time is 0.5h. After the ultrasonic treatment, the solid-liquid separation is performed to obtain a filtrate, the filtrate is concentrated into an extract, and the extract is obtained by spray drying.

[0037] Preparation method of astragalus extract: take astragalus medicinal material, wash, dry, crush, add 6 times the amount of water, heat to boiling and extract twice, each time for 2.5 hours, combine the extracts, concentrate to obtain an extract with a specific gravity of 1.20, and spray dry.

[0038] The preparation method of the Forsythia suspensa extract is as follows: taking Forsythia suspensa medicinal material, washing, drying, crushing, placing in 7 times the amount of water and soaking for 1 hour, heating to boiling, decocting for 2 hours, filtering to obtain an initial extract; adding 6 times the amount of 60% ethanol solution by volume to the initial extract, distilling under reduced pressure to obtain a concentrated solution, and spray drying to obtain the concentrate.

[0039] 10 parts by weight of Houttuynia cordata extract, 7 parts by weight of Astragalus membranaceus extract and 5 parts by weight of Forsythia suspensa extract were mixed to obtain a Chinese medicine mixed extract.

[0040] Example 3

[0041] The preparation method of houttuynia cordata extract is as follows: the houttuynia cordata is dried and crushed, and placed in a 50% ethanol solution with a volume percentage of 3 times the mass, and ultrasonic treatment is performed twice, the power of the first ultrasonic treatment is 190W; the ultrasonic temperature is 55°C; the ultrasonic frequency is 60KHz, and the ultrasonic time is 0.5h. After the ultrasonic treatment, the second ultrasonic treatment is performed after standing for 3h, and the power of the second ultrasonic treatment is 130W; the ultrasonic temperature is 55°C; the ultrasonic frequency is 55KHz, and the ultrasonic time is 0.5h. After the ultrasonic treatment, the solid-liquid separation is performed to obtain the filtrate, and the filtrate is concentrated into an extract, and spray drying is performed to obtain the extract.

[0042] Preparation method of astragalus extract: take astragalus medicinal material, wash, dry, crush, add 5 times the amount of water, heat to boiling and extract twice, each time for 2 hours, combine the extracts, concentrate to obtain an extract with a specific gravity of 1.10, and spray dry.

[0043] The preparation method of the forsythia extract is as follows: taking forsythia medicinal materials, washing, drying, crushing, placing in 6 times the amount of water and soaking for 1 hour, heating to boiling, decocting for 2 hours, filtering to obtain an initial extract; adding 5 times the amount of 50% ethanol solution by volume to the initial extract, distilling under reduced pressure to obtain a concentrated solution, and spray drying to obtain the concentrate.

[0044] 8 parts by weight of Houttuynia cordata extract, 6 parts by weight of Astragalus membranaceus extract and 4 parts by weight of Forsythia suspensa extract were mixed to obtain a Chinese medicine mixed extract.

[0045] Example 4 In vitro antiviral study of Yumai oropharyngeal mixture combined with mixed extracts of traditional Chinese medicine

[0046] Yumai oropharyngeal mixture was provided by Yunnan Shengke Pharmaceutical Co., Ltd.

[0047] Ribavirin granules were provided by Sichuan Baili Pharmaceutical Co., Ltd.

[0048] 4.1 Dosage Grouping

[0049] The mixed Chinese medicinal extract prepared in Example 1 and Yumai oropharyngeal mixture were selected and used in combination at a mass ratio of 0.8:1 as the first administration agent;

[0050] The mixed Chinese herbal extract prepared in Example 1 and Yumai oropharyngeal mixture were selected and used in combination at a mass ratio of 1.3:1 as the second medication;

[0051] The mixed Chinese medicinal extract prepared in Example 1 and Yumai oropharyngeal mixture were selected and used in combination at a mass ratio of 0.5:1 as the third medication;

[0052] Comparative Example 1 is to remove the Houttuynia cordata extract from the mixed Chinese herbal extract prepared in Example 1 and use it in combination with Yumai oropharyngeal mixture at a mass ratio of 0.8:1 as administration group 4;

[0053] Comparative Example 2 is to remove the astragalus extract from the mixed Chinese herbal extract prepared in Example 1 and use it in combination with Yumai oropharyngeal mixture at a mass ratio of 0.8:1 as administration group 5;

[0054] Comparative Example 3 is to remove the Forsythia suspensa extract from the mixed Chinese herbal extract prepared in Example 1 and use it in combination with Yumai oropharyngeal mixture at a mass ratio of 0.8:1 as administration group 6;

[0055] The ribavirin positive control was used as the administration group 7.

[0056] The viruses to be tested are CVB2 and CVA2.

[0057] 4.2 Virus culture

[0058] Hep-2 cell line cryopreservation tubes were taken out of the liquid nitrogen tank and immediately put into 37℃~42℃ warm water to melt. The liquid in the cryopreservation tubes was transferred into centrifuge tubes in the clean bench, centrifuged at 1000r / min for 5min, the cryopreservation liquid was discarded, 8mL of 10% 1640 cell culture medium was added to blow and mix the lower sediment cells, and the cells were transferred to the culture bottle with a pipette and cultured in a constant temperature incubator at 37℃ and 5% CO2. After the cells grow into a monolayer, they were passaged. The viruses were inoculated on the Hep-2 cells that had grown into a monolayer, and 8mL of RPMI-1640 maintenance solution was added and placed in a 37℃, 5% CO2 virus incubator for culture. Cell controls were set up and observed daily. When the cells showed more than 80% of the lesions, they were frozen and thawed repeatedly 3 times, gently blown with a pipette, centrifuged at 1000r / min for 5min, and the supernatant was quantitatively divided into small green bottles, sealed with tape, and frozen in a -80℃ refrigerator for standby use.

[0059] 4.3 Virulence testing

[0060] The virus was diluted 10 times with RPMI-1640 maintenance solution to different concentrations, repeated 4 wells vertically, and inoculated into 96-well plates of Hep-2 cells that had grown into a monolayer, and a cell control was set up at the same time. Cultured in a 37°C, 5% CO2 virus incubator, observed daily under an inverted microscope, and observed continuously for 5 days. The liquid in the plate wells was aspirated and discarded, 50μL of 1% neutral red was added, and incubated in a 37°C, 5% CO2 incubator for 1 hour, the staining solution was discarded, the excess dye was fully washed with washing solution, 100μL of decolorizing solution was added, and decolorization was performed at room temperature for 10 minutes. The OD value was measured at a wavelength of 540mn using an enzyme marker. The half infection concentration TCID50 of the virus solution was calculated according to the Reed-Muench formula.

[0061] Cell survival rate = OD value of each group / OD value of normal cells × 100%

[0062] Cytopathic rate = 1 - cell survival rate

[0063] Cell ratio distance = (lesion rate above 50% - 50%) / (lesion rate above 50% - lesion rate below 50%) × 100%

[0064] TCID 50 =Antilog(log higher than 50% CPE percentage virus dilution + ratio × logarithm of dilution factor)

[0065] According to TCID 50 As a result, the virus stock solution was diluted to 10 -6 -10 -7 use.

[0066] 4.4 Determination of drug cytotoxicity

[0067] Each group of drugs to be tested in 4.1 was diluted with RPMI-1640 maintenance solution in two-fold ratios to 7 concentration gradients, and inoculated into 96-well plates of Hep-2 cells that had grown into a monolayer, with 4 duplicate wells and a cell control well; cultured in a 37°C, 5% CO2 incubator, and the cell lesions were observed daily for 3 consecutive days to calculate the maximum non-toxic concentration of the drug. Cells with lesions were judged to be drug toxic, stained with neutral red, and the OD value was measured at a wavelength of 540nm using an enzyme marker, and the Reed-Muench formula was used to calculate the drug half-toxic concentration (TC 50 ) and determine the minimum non-toxic concentration (TC0). TC 50 =[Antilog(log virus dilution above 50% CPE percentage + ratio)]×C

[0068] 4.5 Antiviral experiments

[0069] Each group of tested drugs was diluted in RPMI-1640 two-fold serial dilutions to 7 concentrations starting from the non-toxic concentration. 50 μL per well was inoculated into 96-well plates that had grown into a monolayer of Hep-2 cells. 50 μL of virus solution was added to each well. At the same time, a ribavirin positive control group, a virus control group, and a blank cell control group were set up, with 4 replicates in each group. An equal amount of blank culture medium was added to the virus control group and the blank cell group. The cells were cultured in a 5% CO2 incubator and the cell lesions were observed. When the virus control showed more than 90% lesions, the cells were stained with 1% neutral red and the OD value was measured at a wavelength of 540 nm using an enzyme reader. The Reed-Muench method was used to calculate the drug's median effective concentration (EC 50 )

[0070] The experimental results are shown in Table 1.

[0071] EC 50 =[Antilog(log value of virus dilution with percentage higher than 50% CPE - ratio)]×C

[0072] TI = median toxic concentration (TC 50 ) / half effective concentration (EC 50 )

[0073] Table 1 The therapeutic index of different drug administration groups for CVB2 and CVA2 viruses

[0074] Group CVB2 CVA2 Administer 1 group <![CDATA[71.5 a ]]> <![CDATA[84.3 a ]]> Administer 2 groups <![CDATA[73.8 a ]]> <![CDATA[86.7 a ]]> 3 groups of medication <![CDATA[70.2 a ]]> <![CDATA[83.6 a <!-- 5 -->]]> 4 groups were administered (Comparative Example 1) <![CDATA[46.8 b ]]> <![CDATA[53.4 b ]]> 5 groups of medication (Comparative Example 2) <![CDATA[50.3 b ]]> <![CDATA[58.7 b ]]> 6 groups of medication (Comparative Example 3) <![CDATA[52.4 b ]]> <![CDATA[60.3 b ]]> Group 7 (positive control) <![CDATA[66.5 c ]]> <![CDATA[75.6 c ]]>

[0075] Note: Different letters in the same column indicate significant differences.

[0076] As can be seen from Table 1, the present invention combines Yumai oropharyngeal mixture with Houttuynia cordata extract, Astragalus extract and Forsythia suspensa extract to achieve significant effects against CVA2 and CVB2 viruses. The components of the mixed Chinese medicinal extract provided by the present invention are indispensable. Comparative Examples 1 to 3 lack Houttuynia cordata extract, Astragalus extract and Forsythia suspensa extract, respectively, and their antiviral effects are significantly reduced, indicating that there is a synergistic effect between Yumai oropharyngeal mixture and Houttuynia cordata extract, Astragalus extract and Forsythia suspensa extract. Example 5 Clinical trial of Yumai oropharyngeal mixture combined with mixed Chinese medicinal extracts

[0077] 5.1 Clinical Data

[0078] A total of 76 children with herpangina admitted to Chengdu High-tech Good Doctor First Hospital from January 2019 to December 2021 were collected as research subjects, and the patients were randomly divided into a treatment group and a control group.

[0079] 5.2 Inclusion criteria

[0080] (1) Clinical diagnosis of herpetic pharyngitis, fever, dysphagia, gray-white herpes in the pharyngeal isthmus, meeting the relevant diagnostic criteria, age ≤ 7 years old; (2) The parents of the children were aware of the study content, cooperated with the treatment, and signed the informed consent. (3) Exclusion criteria: patients with hand, foot and mouth disease, severe congenital diseases, autoimmune diseases, major organ dysfunction, bacterial infectious diseases, and allergies to the drugs used in this study. (4) The children were randomly divided into a treatment group and a control group, with 38 cases in each group. There were 20 males and 18 females in the treatment group; the age ranged from 2 to 7 years, with an average age of (4.63±1.15) years; the course of disease was 1 to 6 days, with an average course of disease of (3.49±0.72) days; the body temperature was 37.6 to 40.3℃, with an average body temperature of (38.57±0.53)℃. In the control group, there were 21 males and 17 females; the age ranged from 2 to 7 years old, with an average age of (4.58±1.13) years old; the course of disease ranged from 1 to 6 days, with an average course of disease of (3.51±0.68) days; the body temperature ranged from 37.5 to 40.1℃, with an average body temperature of (38.55±0.54)℃. There was no significant difference in general data such as gender, age, and course of disease between the two groups (P>0.05), and the two groups were comparable.

[0081] 5.3 Administration

[0082] The treatment group used the mixed Chinese herbal extract prepared in Example 1 in combination with Yumai oropharyngeal mixture, wherein the mass ratio of the mixed Chinese herbal extract to Yumai oropharyngeal mixture was 0.8:1, the total amount did not exceed 50 g per day, and was administered in 3 divided doses.

[0083] The control group took cefaclor, 20-40 mg / kg per day, divided into 3 doses, with the total daily dose not exceeding 1 g. The two groups took the medicine for 7 days, and the improvement of the patients' symptoms was observed.

[0084] 5.4 Efficacy judgment:

[0085] The treatment effects of the two groups were compared. Markedly effective: within 3 days of treatment, the body temperature of the children dropped to the normal range, there was no herpes in the pharyngeal isthmus, and the symptoms of pharyngitis, congestion, and drooling disappeared; effective: the symptoms of pharyngitis, congestion, etc. were improved, with a few herpes, and turned into low fever within 3 days of treatment; ineffective: the symptoms of herpetic pharyngitis did not improve or worsened, the fever did not ease for more than 3 days, the pharyngitis improved for more than 5 days, or the herpes did not heal and formed ulcers.

[0086] Table 2 Results of the combined use of the Chinese medicine mixed extract of the present invention and Yumai oral and pharyngeal mixture to treat herpetic pharyngitis in children

[0087] Group Significant effect efficient invalid Efficiency(%) Total effective rate (%) Treatment Group 32 4 2 84 94.7 Control group 28 6 4 76 89.5

[0088] As shown in Table 2, the Yumai oropharyngeal mixture provided by the present invention combined with Houttuynia cordata extract, Astragalus extract and Forsythia suspensa extract in the treatment of herpetic pharyngitis in children is significantly better than that of the control group.

Claims

1. Use of Yumai oropharyngeal mixture combined with a mixed Chinese medicine extract in the preparation of a drug for treating stomatitis; the mixed Chinese medicine extract is obtained by mixing a houttuynia cordata extract, an astragalus extract and a forsythia suspensa extract.

2. The use according to claim 1, characterized in that: The weight proportions of the extracts in the mixed Chinese herbal extract are as follows: 6 to 12 weight proportions of Houttuynia cordata extract, 3 to 8 weight proportions of Astragalus membranaceus extract, and 1 to 6 weight proportions of Forsythia suspensa extract.

3. The use according to claim 1, characterized in that: The Yumai oropharyngeal mixture is used in combination with a mixed extract of traditional Chinese medicine, and the mass ratio of the mixed extract of traditional Chinese medicine to the Yumai oropharyngeal mixture is 0.5-1.5:

1.

4. The use according to claim 1 or 2, characterized in that: The preparation method of the houttuynia cordata extract comprises the following steps: drying and crushing the houttuynia cordata, placing the houttuynia cordata in an ethanol solution for ultrasonic extraction, separating the solid from the liquid to obtain a filtrate, concentrating the filtrate into an extract, and spray drying the extract.

5. The use according to claim 1 or 2, characterized in that: The preparation method of the astragalus extract is as follows: taking astragalus medicinal materials, adding water to boil, standing, filtering, combining supernatants, concentrating to a thick paste, and spray drying to obtain the extract.

6. The use according to claim 1 or 2, characterized in that: The preparation method of the forsythia extract comprises the following steps: crushing the forsythia, soaking it in water, decocting it, filtering it to obtain an initial extract; adding ethanol to the initial extract, performing reduced pressure distillation to obtain a concentrated solution, and spray drying it.

7. The use according to claim 1, characterized in that: The stomatitis is herpetic pharyngitis, oral ulcer, pharyngitis, periodontitis, gingivitis.

8. The use according to claim 7, characterized in that: The stomatitis is herpetic pharyngitis.