Dendrobium officinale leaf extract as well as preparation method and application thereof

Abstract

The invention provides a Dendrobium officinale Kimura amp (MIG) leaf extract, a preparation method of the Dendrobium officinale Kimura amp (MIG) leaf extract, and application of the Dendrobium officinale Kimura amp (MIG) leaf extract in the fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 have the effect of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, HDFa), and can be used for preparing anti-aging drugs or skin care products, so that the dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 can be used for preparing anti-aging drugs or skin care products.

Description

Technical Field

[0001] The present invention belongs to the field of cosmetics and pharmaceutical technology, specifically to the field of botanical medicine technology, and more specifically to Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, pharmaceutical compositions containing the extracts, cosmetics containing the extracts, preparation methods thereof, and applications thereof in the preparation of drugs and cosmetics. Background Art

[0002] Collagen is a major structural protein in the extracellular matrix of mammals. It is widely present in skin, bones, muscles and other tissues. It is mainly involved in physiological and biochemical behaviors such as cell proliferation, differentiation, migration and signal transduction, and plays a supporting, repairing and protecting role for tissue cells [Ye Tao, Xiang Qi, Yang Yan, Huang Yadong. Research progress on the development and application of collagen. Journal of Biotechnology, 2023, 39(3): 942–960]. Among them, type I collagen is the most abundant protein in human skin, accounting for about 70% of the dry weight of skin. Type I collagen can increase skin elasticity and delay skin aging [Zhang Yuhan, Zhou Jingxuan, Zhang Nanhai, Zhao Liang, Zhang Liebing, Zhou Feng. Research progress on collagen peptides and elastin peptides to improve skin photoaging. Food Research and Development, 2023, 44(11): 208–215]. In addition, on the wound surface, type I collagen promotes wound healing by providing structural support for cell attachment and migration. However, animal-derived type I collagen may be immunogenic, and problems related to the processing technology, degradation rate and sterilization process of type I collagen limit its application [Cheng Y., Li Y., Huang S., Yu F., Bei Y., Zhang Y., Tang J., Huang Y., Xiang Q. Hybrid freeze-dried dressings composed of epidermal growth factor and recombinant human-like collagen enhance cutaneous wound healing in rats. Front. Bioeng. Biotechnol., 2020, 8: 742]. Therefore, screening plant extracts and natural products with the activity of promoting collagen secretion is a current research hotspot.

[0003] Dendrobium officinale Kimura & Migo leaves are food ingredients with the effects of lowering blood sugar, blood lipids, and uric acid, but there are no reports on the activity of Dendrobium officinale leaf extract and its promotion of collagen secretion. Summary of the invention

[0004] In view of the above-mentioned deficiencies in the prior art, the present invention provides Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, pharmaceutical compositions containing the extracts, cosmetics containing the extracts, preparation methods thereof, and applications thereof in the preparation of drugs and cosmetics.

[0005] In order to achieve the above-mentioned object of the present invention, the present invention provides the following technical solutions:

[0006] Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 are prepared by the following methods:

[0007] The dried Dendrobium officinale leaf powder was added with 10% ethanol, and ultrasonic-assisted extraction was performed at 60°C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169.

[0008] The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60°C for 60 minutes with ultrasonic assistance, extracted twice, the extract was filtered, the solvent was recovered, and a crude extract was obtained. The crude extract was added with water to prepare a suspension, extracted three times with an amount of petroleum ether equal to the volume of the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205;

[0009] Take extract WYH202, use 80-100 mesh silica gel, mix the sample in a ratio of sample to silica gel of 1:1, use 100-200 mesh silica gel for column chromatography, the ratio of sample to silica gel is 1:5, use petroleum ether-ethyl acetate system for elution, petroleum ether-ethyl acetate 1:0 elution part, recover the solvent, and obtain extract WYH328.

[0010] A method for preparing Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, the method comprising the following steps:

[0011] The dried Dendrobium officinale leaf powder was added with 10% ethanol, and ultrasonic-assisted extraction was performed at 60°C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169.

[0012] The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60°C for 60 minutes with ultrasonic assistance, extracted twice, the extract was filtered, the solvent was recovered, and a crude extract was obtained. The crude extract was added with water to prepare a suspension, extracted three times with an amount of petroleum ether equal to the volume of the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205;

[0013] Take extract WYH202, use 80-100 mesh silica gel, mix the sample in a ratio of sample to silica gel of 1:1, use 100-200 mesh silica gel for column chromatography, the ratio of sample to silica gel is 1:5, use petroleum ether-ethyl acetate system for elution, petroleum ether-ethyl acetate 1:0 elution part, recover the solvent, and obtain extract WYH328.

[0014] The application of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 in the preparation of anti-aging drugs.

[0015] The pharmaceutical composition is composed of any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 or any combination thereof and a pharmaceutically acceptable carrier.

[0016] The application of the pharmaceutical composition in the preparation of anti-aging drugs.

[0017] The method for preparing the pharmaceutical composition comprises the following steps:

[0018] The dried Dendrobium officinale leaf powder was added with 10% ethanol, and ultrasonic-assisted extraction was performed at 60°C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169.

[0019] The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60°C for 60 minutes with ultrasonic assistance, extracted twice, the extract was filtered, the solvent was recovered, and a crude extract was obtained. The crude extract was added with water to prepare a suspension, extracted three times with an amount of petroleum ether equal to the volume of the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205;

[0020] Take the extract WYH202, use 80-100 mesh silica gel, mix the sample with the silica gel in a ratio of 1:1, use 100-200 mesh silica gel for column chromatography, the sample and silica gel ratio is 1:5, use petroleum ether-ethyl acetate system for elution, petroleum ether-ethyl acetate 1:0 elution part, recover the solvent, and obtain the extract WYH328,

[0021] Then, any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 or any combination thereof is added to a pharmaceutically acceptable carrier.

[0022] The cosmetics use any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 or any combination thereof as an active ingredient, and add conventional cosmetic excipients.

[0023] Application of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 in the preparation of cosmetics.

[0024] The method for preparing the cosmetics comprises the following steps:

[0025] The dried Dendrobium officinale leaf powder was added with 10% ethanol, and ultrasonic-assisted extraction was performed at 60°C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169.

[0026] The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60°C for 60 minutes with ultrasonic assistance, extracted twice, the extract was filtered, the solvent was recovered, and a crude extract was obtained. The crude extract was added with water to prepare a suspension, extracted three times with an amount of petroleum ether equal to the volume of the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205;

[0027] Take the extract WYH202, use 80-100 mesh silica gel, mix the sample with the silica gel in a ratio of 1:1, use 100-200 mesh silica gel for column chromatography, the sample and silica gel ratio is 1:5, use petroleum ether-ethyl acetate system for elution, petroleum ether-ethyl acetate 1:0 elution part, recover the solvent, and obtain the extract WYH328,

[0028] Then, any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 or any combination thereof is added to conventional cosmetic excipients.

[0029] In the present invention, when the extract or its composition is used to prepare a drug, the content of the extract or its composition in the drug is preferably 0.1-99%; in the pharmaceutical composition, the content of any one of the active ingredient extracts or any combination thereof in the pharmaceutical composition is preferably 0.5-90%. The pharmaceutical composition of the present invention is preferably used in the form of a dosage per unit body weight. In the present invention, the prepared drug can preferably be administered in two forms: injection (intravenous injection, intramuscular injection) and oral administration. The pharmaceutical preparations include tablets, capsules, nasal sprays, pills, dripping pills, etc., including but not limited to the above-mentioned preparations.

[0030] In the present invention, when the extract is used to prepare cosmetics, the preparation of the cosmetics is not limited and can be prepared by conventional preparation methods of cosmetics.

[0031] Compared with the prior art, the present invention has the following advantages:

[0032] 1. The present invention provides Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, which have the effect of promoting collagen secretion at a relatively low concentration.

[0033] 2. Dendrobium officinale leaf extracts WYH169, WYH202 and WYH328 have no toxicity to adult dermal fibroblasts at the concentrations where they exert their efficacy.

[0034] 3. The present invention provides a preparation method of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, which has readily available raw materials, simple process, convenient operation and low cost. DETAILED DESCRIPTION

[0035] The following embodiments of the present invention are used to further illustrate the essential content of the present invention, but the present invention is not limited thereto.

[0036] Example 1

[0037] Preparation of Dendrobium officinale leaf extract WYH169:

[0038] 4 g of dried Dendrobium officinale leaf powder was added with 160 mL of 10% ethanol and subjected to ultrasonic-assisted extraction for 30 min at 60° C. The extract was filtered and the solvent was recovered to obtain extract WYH169 (2.0 g).

[0039] Embodiment 2:

[0040] Preparation of Dendrobium officinale leaf extracts WYH202, WYH203, WYH204 and WYH205:

[0041] Take 50g of dried and crushed Dendrobium officinale leaf powder, use 500mL 90% ethanol, at 60℃, ultrasonic-assisted extraction for 60min, extract twice, and filter the extract. Recover the solvent to obtain 7.5g of crude extract. Add 12mL of water to the crude extract to prepare a suspension, extract 3 times with petroleum ether equal to the volume of the suspension, and recover the solvent from the extract (organic phase) to obtain extract WYH202 (2.5g). The aqueous phase after petroleum ether extraction is extracted 3 times with an equal volume of ethyl acetate, and the extract (organic phase) is recovered to obtain extract WYH203 (0.2g). The aqueous phase after ethyl acetate extraction is extracted 3 times with an equal volume of n-butanol, and the extract (organic phase) is recovered to obtain extract WYH204 (1.0g). The aqueous phase after n-butanol extraction is recovered to obtain extract WYH205 (3.7g).

[0042] Embodiment 3:

[0043] Preparation of Dendrobium officinale leaf extracts WYH328, WYH329, WYH330 and WYH331:

[0044] Take 2.0g of extract WYH202, mix it with 80-100 mesh silica gel at a ratio of 1:1 between sample and silica gel. Use 100-200 mesh silica gel for column chromatography, with a sample to silica gel ratio of 1:5. Elution is performed with a petroleum ether-ethyl acetate system. After recovering the solvent from the petroleum ether-ethyl acetate (1:0) elution portion, WYH328 (0.1g) is obtained; after recovering the solvent from the petroleum ether-ethyl acetate (10:1) elution portion, WYH329 (0.4g) is obtained; after recovering the solvent from the petroleum ether-ethyl acetate (3:1) elution portion, WYH330 (0.1g) is obtained; after recovering the solvent from the petroleum ether-ethyl acetate (0:1) elution portion, WYH331 (0.4g) is obtained.

[0045] Example 4

[0046] Extracts promote collagen secretion experiment.

[0047] 1. Cells

[0048] Human dermal fibroblasts-adult (HDFa) were purchased from Beina Biotechnology.

[0049] 2. Reagents

[0050] DMEM (high glucose) medium, PBS, Hank's balanced salt solution (HBSS), penicillin-streptomycin and fetal bovine serum (FBS) were purchased from Hyclone; 0.25% trypsin (containing EDTA) was purchased from ThermoFisher; transforming growth factor beta (TGF-β) was purchased from Peprotech; collagen ELISA kit was purchased from TaKaRa; MTS reagent was purchased from Promega.

[0051] 3. Experimental Methods

[0052] On a 96-well cell culture plate, HDFa cells were mixed with the sample to be tested, and a blank control without extract and a TGF-β positive control were set up; cultured at 37°C, 5% CO2 for 3 days, the cell culture supernatant was collected and stored at -80°C; MTS was added, and the OD value at 490nm was detected by MTS colorimetry; the secretion of collagen was detected according to the method provided in the collagen ELISA kit, and the OD value was measured by a microplate reader, and the detection wavelength was 450nm. The increase rate of collagen secretion was calculated.

[0053] Collagen secretion increase rate (%) = (OD 450 nm / Cell viability / blank well OD 450 nm -1)×100%

[0054] 4. Experimental Results

[0055] The present invention evaluates the activity of 9 Dendrobium officinale leaf extracts in promoting the secretion of HDFa collagen, and the test results are shown in Table 1. At a concentration of 10 μg / mL, extracts WYH169, WYH202 and WYH205 have a significant promoting effect on collagen secretion. At a concentration of 1 μg / mL, extract WYH328 has a significant promoting effect on collagen secretion.

[0056] Table 1. Effect of Dendrobium officinale leaf extract on the secretion of HDFa collagen

[0057]

[0058] Embodiment 5:

[0059] Excipients are added in a weight ratio of 1:1 between any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 or any combination thereof and the excipients, and granulation and tableting are performed.

[0060] Embodiment 6:

[0061] Excipients are added in a weight ratio of 1:2 between any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 or any combination thereof and the excipients, and granulation and tableting are performed.

[0062] Embodiment 7:

[0063] Tablets: 10 mg of any one or any combination of Dendrobium officinale leaf extract WYH169, WYH202, WYH205 and WYH328, 180 mg of lactose, 55 mg of starch, and 5 mg of magnesium stearate;

[0064] Preparation method: any one or any combination of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, lactose and starch are mixed, and the mixture is evenly moistened with water. The moistened mixture is sieved and dried, and then sieved again. Magnesium stearate is added, and then the mixture is compressed into tablets, each tablet weighing 250 mg and containing 10 mg of the compound.

[0065] Embodiment 8:

[0066] Any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 or any combination thereof is prepared into capsules according to a conventional capsule preparation method.

[0067] Embodiment 9:

[0068] Capsules: 10 mg of any one or any combination of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328, 187 mg of lactose, and 3 mg of magnesium stearate;

[0069] Preparation method: Mix any one or any combination of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 with adjuvants, sieve, mix evenly, and fill the obtained mixture into hard gelatin capsules, each capsule weighing 200 mg and containing 10 mg of active ingredients.

[0070] Embodiment 10:

[0071] Ampoule: 2 mg of any one or any combination of Dendrobium officinale leaf extract WYH169, WYH202, WYH205 and WYH328, 10 mg of sodium chloride;

[0072] Preparation method: dissolve any one or any combination of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 and sodium chloride in an appropriate amount of water for injection, filter the resulting solution, and fill it into an ampoule bottle under sterile conditions.

[0073] Embodiment 11:

[0074] Cream formula containing any one of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 or any combination thereof (W%):

[0075]

[0076]

[0077] The cosmetics of the present invention are prepared by conventional methods for preparing cosmetics.

[0078] Embodiment 12:

[0079] Emulsion formula containing any one of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 or any combination thereof (W%):

[0080]

[0081] The cosmetics of the present invention are prepared by conventional methods for preparing cosmetics.

[0082] Example 13

[0083] A cleanser with Dendrobium officinale leaf extract.

[0084] 1. Materials

[0085] The Dendrobium officinale leaf extract obtained by the method of Example 1 uses cosmetic grade or food grade raw materials.

[0086] 2. Formulation

[0087] 0.5g of any one of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 or any combination thereof, 3.0g of glycerin, 3.0g of butylene glycol, 0.6g of propylene glycol, 0.1g of sodium EDTA, 0.3g of guar gum, 2.0g of zinc dioxide, 4.0g of C12-15 alcohol benzoate, 3.0g of C12-20 alkyl glucoside, 0.5g of C14-22 alcohol, 1.2g of cetearyl alcohol, 0.2g of paraben, 0.3g of sodium stearate, 0.5g of dimethicone alcohol, 0.2g of polysorbate, and 60g of deionized water.

[0088] 3. Preparation process

[0089] Weigh any one or any combination of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 in a formula amount, add purified water to completely dissolve; take glycerin, butylene glycol, propylene glycol, sodium EDTA, guar gum, zinc dioxide, sodium stearate, C12-15 benzoate, C12-20 alkyl glucoside, C14-22 alcohol, cetearyl alcohol, add purified water to mix; take paraben, polydimethylsiloxane alcohol, polysorbate, add purified water to mix. Mix the above-prepared solutions, stir well, and can.

Claims

1. Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, WYH328, characterized in that: They are prepared by the following method: The dried Dendrobium officinale leaf powder was added with 10% ethanol and subjected to ultrasound-assisted extraction at 60 °C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169. The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60 °C for 60 min with ultrasonic assistance, extracted twice, filtered the extract, recovered the solvent, and obtained a crude extract. The crude extract was added with water to prepare a suspension, extracted three times with an equal volume of petroleum ether to the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205; Take extract WYH202, use 80-100 mesh silica gel, mix the sample in a ratio of sample to silica gel of 1:1, use 100-200 mesh silica gel for column chromatography, the ratio of sample to silica gel is 1:5, use petroleum ether-ethyl acetate system for elution, petroleum ether-ethyl acetate 1:0 elution part, recover the solvent, and obtain extract WYH328.

2. The preparation method of Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 is characterized in that: The method comprises the following steps: The dried Dendrobium officinale leaf powder was added with 10% ethanol and subjected to ultrasound-assisted extraction at 60 °C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169. The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60 °C for 60 min with ultrasonic assistance, extracted twice, filtered the extract, recovered the solvent, and obtained a crude extract. The crude extract was added with water to prepare a suspension, extracted three times with an equal volume of petroleum ether to the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205; Take extract WYH202, use 80-100 mesh silica gel, mix the sample in a ratio of sample to silica gel of 1:1, use 100-200 mesh silica gel for column chromatography, the ratio of sample to silica gel is 1:5, use petroleum ether-ethyl acetate system for elution, petroleum ether-ethyl acetate 1:0 elution part, recover the solvent, and obtain extract WYH328.

3. Use of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 according to claim 1 in the preparation of anti-aging drugs.

4. A pharmaceutical composition, consisting of any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 according to claim 1 or any combination thereof and a pharmaceutically acceptable carrier.

5. Use of the pharmaceutical composition according to claim 4 in the preparation of anti-aging drugs.

6. The method for preparing the pharmaceutical composition according to claim 4, characterized in that: The method comprises the following steps: The dried Dendrobium officinale leaf powder was added with 10% ethanol and subjected to ultrasound-assisted extraction at 60 °C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169. The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60 °C for 60 min with ultrasonic assistance, extracted twice, filtered the extract, recovered the solvent, and obtained a crude extract. The crude extract was added with water to prepare a suspension, extracted three times with an equal volume of petroleum ether to the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205; Take the extract WYH202, mix the sample with 80-100 mesh silica gel at a ratio of 1:1, perform column chromatography with 100-200 mesh silica gel at a ratio of 1:5, and elute with a petroleum ether-ethyl acetate system at a ratio of 1:0 petroleum ether-ethyl acetate. After recovering the solvent, extract WYH328 is obtained. Then, any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 or any combination thereof is added to a pharmaceutically acceptable carrier.

7. Cosmetics, comprising any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 as claimed in claim 1 or any combination thereof as an active ingredient, and conventional cosmetic excipients.

8. Use of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205 and WYH328 according to claim 1 in the preparation of cosmetics.

9. The method for preparing the cosmetics according to claim 7, characterized in that: The method comprises the following steps: The dried Dendrobium officinale leaf powder was added with 10% ethanol and subjected to ultrasound-assisted extraction at 60 °C for 30 min. The extract was filtered and the solvent was recovered to obtain extract WYH169. The dried and crushed Dendrobium officinale leaf powder was extracted with 90% ethanol at 60 °C for 60 min with ultrasonic assistance, extracted twice, filtered the extract, recovered the solvent, and obtained a crude extract. The crude extract was added with water to prepare a suspension, extracted three times with an equal volume of petroleum ether to the suspension, and the organic phase of the extract was solvent recovered to obtain extract WYH202; the aqueous phase after petroleum ether extraction was extracted three times with an equal volume of ethyl acetate, the aqueous phase after ethyl acetate extraction was extracted three times with an equal volume of n-butanol, and the aqueous phase after n-butanol extraction was solvent recovered to obtain extract WYH205; Take the extract WYH202, mix the sample with 80-100 mesh silica gel at a ratio of 1:1, perform column chromatography with 100-200 mesh silica gel at a ratio of 1:5, and elute with a petroleum ether-ethyl acetate system at a ratio of 1:0 petroleum ether-ethyl acetate. After recovering the solvent, extract WYH328 is obtained. Then, any one of the Dendrobium officinale leaf extracts WYH169, WYH202, WYH205, and WYH328 or any combination thereof is added to conventional cosmetic excipients.

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