Ginseng and cistanche composition for relieving fatigue and protecting liver injury and preparation method thereof

Abstract

The invention discloses a ginseng and cistanche composition for relieving fatigue and protecting liver injury and a preparation method of the ginseng and cistanche composition, and relates to the technical field of medicines and health-care foods. The composition is prepared from the following raw materials: radix ginseng, herba cistanche, oysters, radix puerariae, rhizoma curcumae longae, fructus schizandrae, black chokeberry fruits, hypericum monogynum, semen cassiae, a glutathione-rich yeast extract, spices, taurine and oligosaccharide. According to the traditional Chinese medicine composition disclosed by the invention, the raw materials are compatible, the effective components of the traditional Chinese medicines are extracted, the extracted active components exert different effects, and a synergistic effect is generated among the various effects, so that the AST and ALT activity and oxidative stress level in the plasma of a mouse with liver injury can be remarkably reduced, and the loaded swimming time of the mouse is prolonged; the serum urea nitrogen and blood lactic acid levels of the mice after exercise are reduced, so that the traditional Chinese medicine composition has the functions of improving liver injury and relieving physical fatigue.

Description

Technical Field

[0001] The invention relates to the technical field of medicine and health food, and more particularly to a ginseng and cistanche composite for relieving fatigue and protecting liver damage and a preparation method thereof. Background Art

[0002] The liver is an important metabolic and detoxification organ in the human body, participating in the body's biological transformation and metabolism. When liver cells are stimulated by endogenous or exogenous factors, they can cause liver cell degeneration and necrosis, causing liver damage. Among them, the causes of acute liver injury are complex and can be induced by a variety of factors such as drugs, alcohol, chemical toxins and viruses. It develops rapidly and can cause obvious liver function abnormalities and induce complications such as liver failure, hepatic encephalopathy and acute kidney injury. Acute liver injury is a special critical state between deterioration and recovery. If diagnosed and treated in time, it is expected to be cured; otherwise, it may deteriorate and even cause liver failure, causing serious damage to the body. Therefore, the diagnosis and treatment of acute liver injury is extremely important. In recent years, the study of the mechanism of liver injury, as well as its prevention and treatment, has become one of the hot spots in biological and medical research at home and abroad.

[0003] At present, there is no effective drug for treating chemical liver damage in Western medicine, and lipid-regulating drugs are mainly used as auxiliary treatment. In the process of Western medicine treatment of liver damage, the drugs used will cause damage to the liver during human metabolism due to the effects of the drugs themselves or their metabolites. Excessive reliance on Western medicine treatment will increase hormones in the body, which will further increase the burden on the liver. Moreover, there are not many Chinese herbal liver-protecting health foods available on the market. As people's health knowledge level continues to improve, their awareness of self-protection of the liver is gradually increasing. Combined with my country's traditional Chinese medicine culture and rich Chinese medicine resources, it is an urgent problem for technical personnel in this field to develop a Chinese patent medicine with Chinese medicine as raw material that has auxiliary protection function for chemical liver damage and can also relieve fatigue, has few side effects, and is easy to take. Summary of the invention

[0004] In view of this, the present invention provides a ginseng and Cistanche deserticola composition for relieving fatigue and protecting liver damage and a preparation method thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: A ginseng and cistanche composite for relieving fatigue and protecting liver damage comprises the following raw materials: ginseng, cistanche, oyster, kudzu root, turmeric, schisandra chinensis, aronia nigra, hypericum perforatum, cassia seed, glutathione-rich yeast extract, taurine and oligosaccharide.

[0006] Preferably, the composition further comprises a fragrance; the fragrance is alanine.

[0007] Preferably, the oligosaccharide is one or more of fructooligosaccharide, xylooligosaccharide, isomaltooligosaccharide and galactooligosaccharide.

[0008] Preferably, the composition is used for relieving fatigue and protecting liver damage.

[0009] Furthermore, the composition comprises the following raw materials in parts by weight: 45-60 parts of ginseng, 40-50 parts of Cistanche deserticola, 35-50 parts of oysters, 35-45 parts of Pueraria root, 30-42 parts of turmeric, 28-38 parts of Schisandra chinensis, 25-31 parts of Aronia nigra fruit, 20-30 parts of Hypericum perforatum, 18-26 parts of Cassia seed, 0.01-0.5 parts of glutathione-rich yeast extract, 0.1-03 parts of spices, 0.1-03 parts of taurine and 5-13 parts of oligosaccharides.

[0010] Preferably, the composition comprises the following raw materials in parts by weight: 52 parts of ginseng, 50 parts of Cistanche deserticola, 35 parts of oysters, 40 parts of Pueraria root, 42 parts of turmeric, 33 parts of Schisandra chinensis, 28 parts of Aronia nigra fruit, 20 parts of Hypericum perforatum, 22 parts of Cassia seed, 0.24 parts of glutathione-rich yeast extract, 0.3 parts of spices, 0.01 parts of taurine and 5 parts of oligosaccharides.

[0011] As an inventive concept identical to the above technical solution, the present invention also requests protection for the use of the above composition or the composition prepared by the above method, which is to prepare medicines, health products or foods for relieving fatigue and protecting liver damage.

[0012] As an inventive concept identical to the above technical solution, the present invention also seeks to protect a pharmaceutical preparation for relieving fatigue and protecting liver damage, wherein the preparation comprises the above composition and a pharmaceutically acceptable carrier.

[0013] Furthermore, the schisandra chinensis is firstly extracted to obtain schisandra chinensis alcohol extract, and then a composition or a pharmaceutical preparation is prepared; the preparation method of the schisandra chinensis alcohol extract is as follows: the schisandra chinensis is dried and then crushed, passed through a 40-60 mesh sieve, and the schisandra chinensis powder is added to an ethanol aqueous solution and soaked according to a ratio of schisandra chinensis powder: ethanol m:v (g / mL) = 1:8-12, reflux extraction, the extracts are combined and centrifuged, and after reduced pressure drying, the schisandra chinensis alcohol extract can be obtained; Preferably, the volume concentration of the ethanol aqueous solution is 70%-90%; the soaking time is 20-40 min; the number of extractions is 2-4 times, and the extraction time is 1-2 h / time; the centrifugation is 1000-1400 rpm for 10-20 min.

[0014] Furthermore, firstly, the Aronia nigra fruit is extracted to obtain Aronia nigra fruit alcohol extract, and then a composition or a pharmaceutical preparation is prepared; the preparation method of the Aronia nigra fruit alcohol extract is as follows: the Aronia nigra fruit is dried and crushed, passed through a 60-80 mesh sieve, and the Aronia nigra fruit is added to an ethanol aqueous solution at a ratio of Aronia nigra: ethanol m:v (g / mL) = 1:20-30, stirred evenly, extracted by ultrasonication in a water bath, filtered, centrifuged, and dried under reduced pressure to obtain the Aronia nigra fruit alcohol extract.

[0015] Preferably, the volume concentration of the ethanol aqueous solution is 70%-80%; the water bath temperature is 60-70°C, the ultrasonic power is 50-100W, the extraction time is 2-4h; and the centrifugation is 600-800rpm for 10-16min.

[0016] As the same inventive concept as the above technical solution, the present invention also claims a method for preparing the above composition, comprising the following steps: (1) Weigh the raw materials according to the above weight proportions and set aside; (2) Ginseng, Cistanche deserticola, and oyster are dried and crushed, and passed through a 60-80 mesh sieve to obtain a mixed powder A. The mixed powder A is added to an ethanol aqueous solution at a ratio of mixed powder A: ethanol m:v (g / mL) = 1:10-14, and the mixed powder A is soaked, refluxed for extraction, and the extracts are combined and centrifuged. After reduced pressure drying, extract I is obtained; (3) Drying and crushing Pueraria root, turmeric, Hypericum perforatum and Cassia seed, and passing through a 40-60 mesh sieve to obtain a mixed powder B, adding the mixed powder B to n-butanol at a ratio of mixed powder B: n-butanol m:v (g / mL) = 1:10-16, stirring evenly, performing ultrahigh pressure assisted extraction, filtering, centrifuging and drying under reduced pressure to obtain extract II; (4) Extract I and extract II are mixed, and Schisandra chinensis extract, Aronia nigra fruit extract, glutathione-rich yeast extract, spices, taurine and oligosaccharide acid are added in sequence, mixed and homogenized, and dried under reduced pressure to obtain a composition.

[0017] Furthermore, in step (2), the volume concentration of the ethanol aqueous solution is 50%-60%; the soaking time is 10-20 min; the number of extractions is 3-5 times, and the extraction time is 1-2 h; and the centrifugation is 550-650 rpm for 5-10 min.

[0018] Furthermore, in step (3), the extraction pressure is 90-110 MPa, the extraction time is 3-7 min, the centrifugation is 800-1200 rpm for 8-12 min, and the reduced pressure drying is performed to a water content of 5-9%.

[0019] Furthermore, the homogenization speed is 1200-1600 rpm, and the homogenization time is 10-18 min.

[0020] It can be seen from the above technical scheme that, compared with the prior art, the present invention discloses a ginseng and Cistanche deserticola composition and a preparation method thereof for relieving fatigue and protecting liver damage, and has the following beneficial effects: The present invention combines the above-mentioned raw materials and extracts effective components of traditional Chinese medicines, so that each extracted active component exerts different effects, wherein ginseng and Cistanche deserticola play a main role, and other traditional Chinese medicines play an auxiliary role, and utilize their effective components to protect the liver and delay the occurrence of fatigue; and, the aronia melanocarpa fruit extract and Hypericum perforatum can produce a synergistic effect, which promotes the abundance of beneficial bacteria in the intestine and enhances the antioxidant capacity of liver cells, thereby inhibiting the occurrence of liver inflammation and improving liver damage.

[0021] The composition of the present invention can significantly reduce the increased activities of AST and ALT in the plasma of mice with liver damage, reduce the level of MDA, and increase the levels of SOD, GSH-PX, and CAT, indicating that the composition of the present invention can effectively reduce the level of oxidative stress in mice with liver damage, and has a protective effect on liver damage; in addition, the composition can also prolong the weighted swimming time of mice, reduce the serum urea nitrogen level of mice after exercise, and reduce the blood lactate level of mice after exercise, thereby achieving the effect of relieving physical fatigue. DETAILED DESCRIPTION

[0022] The following will be described clearly and completely in conjunction with the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Ginseng: sweet, bitter, slightly warm in nature, and enters the heart, lung, spleen, and kidney meridians. It has the effects of replenishing vital energy, nourishing the spleen and lungs, promoting fluid production and quenching thirst, and calming the mind and improving intelligence. The main active ingredient in ginseng is ginsenoside, which has anti-tumor, anti-skin aging, anti-inflammatory, anti-allergic, anti-diabetic, nerve protection, myocardial protection, and liver protection.

[0024] Cistanche deserticola: It tastes sweet and salty, is warm in nature, and enters the kidney and large intestine meridians. It has the effects of nourishing kidney yang, benefiting essence and blood, and moistening the intestines and promoting bowel movements. The ingredients it contains, such as D-mannitol, chitosan sterol, and carotene, can effectively remove the body's metabolic products after strenuous exercise and can delay the occurrence of fatigue.

[0025] Oysters: They are salty in taste, slightly cold in nature, and enter the liver, gallbladder, and kidney meridians. They are rich in amino acids, taurine, and glycogen, which can not only greatly enhance the function of the liver and improve the liver's detoxification ability, but also reduce the accumulation of lactic acid, thereby helping to speed up the elimination of fatigue.

[0026] Pueraria root: sweet and spicy in taste, cool in nature, enters the spleen and stomach meridians, has the effects of relieving muscles and reducing fever, promoting the production of body fluids and quenching thirst, raising yang and stopping diarrhea; it mainly contains flavonoids such as daidzein, daidzein, puerarin, etc., which have liver protection effects.

[0027] Turmeric: It tastes spicy and bitter, is warm in nature, and enters the liver and spleen meridians. The curcumin in turmeric has significant antioxidant, free radical scavenging and lipid peroxidation inhibiting functions. It induces the gene expression of glutathione cysteine ​​ligase, increases its catalytic activity, and leads to an increase in glutathione levels, thereby achieving antioxidant and liver-protecting effects.

[0028] Schisandra extract: It contains active ingredients such as phenols, flavonoids, terpenes, lignans, phenylpropanoids, amino acid derivatives, alkaloids, organic acids and their derivatives, and has anti-cancer, antioxidant, anti-inflammatory, hepatoprotective, and antibacterial pharmacological activities.

[0029] Aronia nigra berry extract: Aronia nigra berry extract is extracted from Aronia nigra berry. It is rich in flavonoids, anthocyanins, polyphenols and other substances. It is effective in treating cancer, diabetes, cardiovascular and cerebrovascular diseases. Studies have shown that it can promote the abundance of beneficial bacteria in the intestines, restore the intestinal mucosal barrier, and thus inhibit liver inflammation.

[0030] Hypericum wilfordii: bitter in taste, cool in nature, enters the heart and liver meridians, has liver protection, anti-inflammatory, anti-thrombotic, antioxidant, and anti-tumor pharmacological effects. Its main medicinal ingredient is flavonoids (hyperoside), which can enhance the antioxidant capacity of liver cells, thereby protecting liver cells from damage caused by lipid peroxides, and enabling liver cells to perform normal compensatory and defensive functions.

[0031] Cassia seed: Its main active ingredients are anthraquinone components, which have good protective effects on various liver injuries such as immune liver damage, lipopolysaccharide-induced liver damage and carbon tetrachloride-induced liver damage, and also have anti-tumor, anti-inflammatory and antioxidant effects.

[0032] Glutathione-rich yeast extract is rich in glutathione, nucleic acid, amino acid nitrogen, β-glucan, mannan, acid-soluble protein and other nutrients, and has the effects of protecting the liver and gallbladder, protecting the liver and detoxifying.

[0033] Alanine is the basic unit of protein and is a non-essential amino acid and glucogenic amino acid for the human body. Since alanine can be directly absorbed by cells, it can quickly eliminate fatigue, boost spirits and improve anti-fatigue ability after taking it.

[0034] Taurine is a special amino acid in the human body, rich in sulfonic acid groups. It can promote the synthesis, secretion and excretion of bile acid, reduce the cytotoxicity of certain free bile acids, effectively remove fatty liver deposits, resist liver fibrosis, protect pancreatic cells, and reduce the incidence of cirrhosis. By supplementing taurine to maintain taurine homeostasis in the liver, it can prevent liver disease and improve liver damage.

[0035] Example 1 A ginseng and cistanche composite for relieving fatigue and protecting liver damage, comprising the following raw materials in parts by weight: 45 parts of ginseng, 45 parts of cistanche, 50 parts of oyster, 35 parts of kudzu root, 37 parts of turmeric, 38 parts of schisandra, 25 parts of aronia suffruticosa fruit, 25 parts of hypericum perforatum, 26 parts of cassia seed, 0.01 parts of glutathione-rich yeast extract (purchased from Hebei Qiansheng Biotechnology Co., Ltd.), 0.15 parts of alanine, 0.3 parts of taurine and 10 parts of oligofructose; The preparation method is as follows: (1) Weigh the raw materials according to the above weight proportions and set aside; (2) Preparation of Schisandra chinensis alcohol extract: Dry and crush the Schisandra chinensis, pass it through a 60-mesh sieve, add the Schisandra chinensis powder into a 70% ethanol aqueous solution at a ratio of Schisandra chinensis powder: ethanol (m:v) = 1 g: 8 mL, and soak for 20 min. Reflux extraction is performed 4 times, each time for 1 h. The extracts are combined, centrifuged at 1000 rpm for 20 min, and dried under reduced pressure to obtain the Schisandra chinensis extract. (3) Preparation of aronia oleracea fruit ethanol extract: drying the aronia oleracea, crushing it, passing it through a 60-mesh sieve, adding the aronia oleracea to an ethanol aqueous solution with a volume concentration of 70% according to the ratio of aronia oleracea: ethanol m:v = 1 g:20 mL, stirring evenly, extracting it in a water bath with ultrasonic wave at 60°C and 50 W for 3 h, filtering it, centrifuging it at 600 rpm for 16 min, and drying it under reduced pressure to obtain the aronia oleracea fruit ethanol extract; (4) Ginseng, Cistanche deserticola, and oyster were dried and crushed, and passed through an 80-mesh sieve to obtain a mixed powder A. The mixed powder A was added to an ethanol aqueous solution with a volume concentration of 55% according to the ratio of mixed powder A: ethanol m:v = 1g:12mL, and the mixed powder A was soaked for 10 minutes. The mixture was refluxed for 3 times, each time for 1.5 hours. The extracts were combined, centrifuged at 650 rpm for 5 minutes, and dried under reduced pressure until the water content was 7%, thereby obtaining extract I; (5) Dry and crush Pueraria root, turmeric, Hypericum perforatum and Cassia seed, and pass through a 40-mesh sieve to obtain a mixed powder B. Add the mixed powder B to n-butanol at a ratio of mixed powder B: n-butanol (m:v) = 1 g: 13 mL, stir evenly, perform ultrahigh pressure assisted extraction at a pressure of 110 MPa for 3 min, filter, centrifuge at 1200 rpm for 8 min, and dry under reduced pressure to obtain extract II. (6) Extract I and extract II are mixed, and Schisandra chinensis extract, Aronia nigra fruit extract, glutathione-rich yeast extract, alanine, taurine and oligofructose are added in sequence, mixed and homogenized at a rotation speed of 1200 rpm for 18 minutes, and dried under reduced pressure to obtain a composite.

[0036] Example 2 A ginseng and cistanche composite for relieving fatigue and protecting liver damage, comprising the following raw materials in parts by weight: 52 parts of ginseng, 50 parts of cistanche, 35 parts of oyster, 40 parts of kudzu root, 42 parts of turmeric, 33 parts of schisandra, 28 parts of aronia suffruticosa fruit, 20 parts of hypericum, 22 parts of cassia seed, 0.24 parts of glutathione-rich yeast extract (purchased from Hebei Qiansheng Biotechnology Co., Ltd.), 0.3 parts of alanine, 0.01 parts of taurine and 5 parts of oligofructose; The preparation method is as follows: (1) Weigh the raw materials according to the above weight proportions and set aside; (2) Preparation of Schisandra chinensis alcohol extract: Dry and crush Schisandra chinensis, pass through a 50-mesh sieve, add Schisandra chinensis powder to a 90% ethanol aqueous solution at a ratio of Schisandra chinensis powder: ethanol m:v = 1g:10mL, and soak for 30 minutes. Reflux extraction 3 times, each extraction for 1.5 hours, combine the extracts, centrifuge at 1400 rpm for 10 minutes, and dry under reduced pressure to obtain Schisandra chinensis alcohol extract; (3) Preparation of aronia oleracea fruit ethanol extract: drying the aronia oleracea, crushing it, passing it through an 80-mesh sieve, adding the aronia oleracea to an ethanol aqueous solution with a volume concentration of 80% according to the ratio of aronia oleracea: ethanol m:v = 1 g:25 mL, stirring evenly, extracting by ultrasonic in a water bath at 65°C and 70 W for 4 h, filtering, centrifuging at 700 rpm for 13 min, and drying under reduced pressure to obtain the aronia oleracea fruit ethanol extract; (4) Ginseng, Cistanche deserticola, and oyster were dried and crushed, and passed through a 60-mesh sieve to obtain a mixed powder A. The mixed powder A was added to a 50% ethanol aqueous solution with a volume concentration of 1 g:10 mL, and the mixed powder A was soaked for 15 min. The mixture was refluxed for 4 times, each time for 2 h. The extracts were combined, centrifuged at 550 rpm for 10 min, and dried under reduced pressure until the water content was 5%, thereby obtaining extract I. (5) Dry and crush Pueraria root, turmeric, Hypericum perforatum and Cassia seed, and pass through a 50-mesh sieve to obtain a mixed powder B. Add the mixed powder B to n-butanol at a ratio of mixed powder B: n-butanol (m:v) = 1 g: 10 mL, stir evenly, perform ultrahigh pressure assisted extraction at a pressure of 90 MPa for 7 min, filter, centrifuge at 1000 rpm for 10 min, and dry under reduced pressure to obtain extract II. (6) Extract I and extract II are mixed, and Schisandra chinensis extract, Aronia nigra fruit extract, glutathione-rich yeast extract, alanine, taurine and oligofructose are added in sequence, mixed and homogenized at a rotation speed of 1400 rpm for 14 minutes, and dried under reduced pressure to obtain a composite.

[0037] Example 3 A ginseng and cistanche composite for relieving fatigue and protecting liver damage, comprising the following raw materials in parts by weight: 60 parts of ginseng, 40 parts of cistanche, 43 parts of oyster, 45 parts of kudzu root, 32 parts of turmeric, 28 parts of schisandra, 31 parts of aronia suffruticosa fruit, 30 parts of hypericum, 18 parts of cassia seed, 0.5 parts of glutathione-rich yeast extract (purchased from Hebei Qiansheng Biotechnology Co., Ltd.), 0.01 parts of alanine, 0.15 parts of taurine and 13 parts of oligofructose; The preparation method is as follows: (1) Weigh the raw materials according to the above weight proportions and set aside; (2) Preparation of Schisandra chinensis alcohol extract: Dry and crush Schisandra chinensis, pass through a 40-mesh sieve, add Schisandra chinensis powder to an 80% ethanol aqueous solution at a ratio of Schisandra chinensis powder: ethanol m:v = 1 g: 12 mL, and soak for 40 min. Reflux extraction is performed twice, each time for 2 h. The extracts are combined, centrifuged at 1200 rpm for 15 min, and dried under reduced pressure to obtain Schisandra chinensis alcohol extract. (3) Preparation of aronia oleracea fruit ethanol extract: drying the aronia oleracea, crushing it, passing it through a 70-mesh sieve, adding the aronia oleracea to an ethanol aqueous solution with a volume concentration of 75% according to the ratio of aronia oleracea: ethanol m:v = 1 g:30 mL, stirring evenly, extracting it in a water bath with ultrasonic wave at 70°C and 100 W for 2 h, filtering it, centrifuging it at 800 rpm for 10 min, and drying it under reduced pressure to obtain the aronia oleracea fruit ethanol extract; (4) Ginseng, Cistanche deserticola, and oyster were dried and crushed, and passed through a 70-mesh sieve to obtain a mixed powder A. The mixed powder A was added to an ethanol aqueous solution with a volume concentration of 60% according to the ratio of mixed powder A: ethanol m:v = 1 g: 14 mL, and soaked for 20 min. Reflux extraction was performed 5 times, each time for 1 h. The extracts were combined, centrifuged at 600 rpm for 8 min, and dried under reduced pressure until the water content was 9% by mass, thereby obtaining extract I. (5) The kudzu root, turmeric, schisandra chinensis extract, hypericum perforatum, and cassia seed were dried and crushed, and passed through a 60-mesh sieve to obtain a mixed powder B. The mixed powder B was added to n-butanol at a ratio of mixed powder B: n-butanol m:v = 1 g: 16 mL, and the mixture was stirred evenly. The mixture was subjected to ultrahigh pressure assisted extraction at a pressure of 100 MPa for 5 min, filtered, centrifuged at 800 rpm for 12 min, and dried under reduced pressure to obtain extract II. (6) Extract I and extract II are mixed, and Schisandra chinensis ethanol extract, Aronia nigra fruit extract, glutathione-rich yeast extract, alanine, taurine and oligofructose are added in sequence, mixed and homogenized at a rotation speed of 1600 rpm for 10 minutes, and dried under reduced pressure to obtain a composite.

[0038] Experimental Example 1: Auxiliary protective effects of different compositions on chemical liver injury (1) Test method 210 Kunming mice (weighing 18-22 g) were selected and adaptively fed in an environment of 22-25°C for 7 days, and then randomly divided into a blank group, a model group (LPS, 1 mg / kg), a positive control group (bifendate drops, 2 mg / kg), Example 1-3 groups (20 mg / kg), and an experimental AE group (20 mg / kg), with 10 mice in each group. Except for the blank group and the model group, the other 9 groups were gavaged according to the corresponding drug dose, once / d, for 30 consecutive days. 2 hours after the last administration, an acute liver injury model was established by intraperitoneal injection of LPS (1 mg / kg). The blank group was given an equal volume of normal saline and fasted with free drinking water during the period.

[0039] Test group A is based on the composition of Example 2, except that ginseng, Cistanche deserticola and Pueraria root are removed, and the rest are the same; Test group B is based on the composition of Example 2, except that oyster, schisandra chinensis and cassia seed are removed, and the rest is the same; Test group C is based on the composition of Example 2, except that the Aronia melanocarpa fruit extract is removed. The composition includes the following raw materials in parts by weight: 52 parts of ginseng, 50 parts of Cistanche deserticola, 35 parts of oyster, 40 parts of Pueraria root, 42 parts of turmeric, 33 parts of Schisandra chinensis, 48 ​​parts of Hypericum perforatum, 22 parts of Cassia seed, 0.24 parts of glutathione-rich yeast extract, 0.3 parts of alanine, 0.01 parts of taurine and 5 parts of oligofructose; Test group D is based on the composition of Example 2, except that Hypericum wilfordii was removed. The composition includes the following raw materials in parts by weight: 52 parts of ginseng, 50 parts of Cistanche deserticola, 35 parts of oyster, 40 parts of Pueraria root, 42 parts of turmeric, 33 parts of Schisandra chinensis, 48 ​​parts of Aronia nigra fruit extract, 22 parts of Cassia seed, 0.24 parts of glutathione-rich yeast extract, 0.3 parts of alanine, 0.01 parts of taurine and 5 parts of oligofructose; Experimental group E is the composition in publication number CN105395702A, which comprises the following components in parts by mass: 12 parts of cordyceps polysaccharide, 8 parts of antrodia cinnamomea polysaccharide, 2 parts of American ginseng polysaccharide, 1 part of wolfberry polysaccharide, 0.5 parts of versicolor polysaccharide, 1 part of tuckahoe polysaccharide, and 60 parts of xylitol.

[0040] (2) Effects of different compositions on mouse body weight and liver coefficient 12 h after LPS administration, the mice were killed by cervical dislocation, and the livers of each group were quickly removed and weighed to calculate the liver coefficient (organ weight / body weight after the test). The measurement results are shown in Table 1.

[0041] Table 1 Effects of different compositions on mouse body weight and liver coefficient

[0042] As shown in Table 1, after 30 days of administration, the body weight of animals in each group increased, and there was no significant difference in body weight between the groups of Examples 1-3 and the blank group, indicating that the composition of the present invention had no effect on the body weight of mice. Compared with the blank group, the liver coefficient of the mice in the model group was significantly increased, indicating that the liver may be enlarged. After 30 days of administration, the positive control group, Examples 1-3, and the test groups AE can significantly reduce the liver coefficient of the mice, and the effect of reducing the liver coefficient of the mice in Example 2 is the best; after removing some raw materials from the test group AB, although the degree of reduction in the liver coefficient of the mice after administration is higher than that of the Example 1-3 groups, it is significantly lower than the model group, indicating that the removed raw materials play a major role in reducing the liver coefficient of the mice; compared with the test example CD group, it removes the black fruit glandular rib aronia fruit extract and Hypericum, and the degree of reduction of the liver coefficient of the mice is significantly increased compared with Example 2, which indicates that there is a synergistic effect between the black fruit glandular rib aronia fruit extract and Hypericum, and the synergistic effect can significantly reduce the liver coefficient of the mice; the test group E is a composition in the prior art, which can also reduce the liver coefficient of the mice, but the degree of reduction is significantly lower than that of the composition of the present invention.

[0043] (3) Effects of different compositions on ALT and AST in plasma of mice with acute liver injury induced by LPS Blood was collected from the eyeball 12 hours after LPS administration, and the collected blood was centrifuged to separate the serum (4°C, 4000r / min, 10min). The supernatant was taken and the ALT and AST levels were determined according to the instructions of the kit. The results are shown in Table 2.

[0044] Table 2 Effects of different compositions on ALT and AST in mouse plasma

[0045] As can be seen from Table 2, compared with the blank group, the ALT and AST levels in the model group were significantly increased, indicating that LPS-induced mice had obvious liver damage; compared with the model group, the positive control group, Example 1-3 groups, and the test group AE group can significantly reduce the increase in plasma AST and ALT activity caused by LPS, and the effect of the composition group of the present invention is better than that of the test group AD, indicating that the composition group of the present invention can better improve liver damage; Test Example E group is the prior art, although it can also reduce the increase in plasma AST and ALT activity caused by LPS, the effect produced is not as good as the present invention.

[0046] (4) Effects of different compositions on SOD, MDA, GSH-PX, and CAT in LPS-induced acute liver injury tissues After the mice were killed 12 hours after LPS administration, the left lobe of the liver was taken out and homogenized with normal saline to determine the contents of SOD, MDA, GSH-PX and CAT in the liver homogenate. The results are shown in Table 3.

[0047] Table 3 Effects of different compositions on SOD, MDA, GSH-PX and CAT in mouse liver tissue

[0048] CAT is the most efficient endogenous antioxidant enzyme in liver cells for removing reactive oxygen species and is a sensitive marker for tissue oxidative stress levels. MDA is the final oxidation product of the lipid peroxidation process in the body. Excessive levels of MDA will aggravate liver cell damage and necrosis and promote the release of inflammatory factors such as TNF-α, IL-6 and IL-1β, which in turn promote oxidative stress and further aggravate liver damage. At the same time, SOD and GSH-Px present in the body can protect the body from damage by free radicals and reactive oxygen oxidative stress.

[0049] As can be seen from Table 3, compared with the blank group, the MDA level in the model group was significantly increased, and the SOD, GSH-PX, and CAT levels were significantly decreased; compared with the model group, the positive control group, Example 1-3 groups, and the experimental group AE group can significantly reduce the MDA level, increase the SOD, GSH-PX, and CAT levels, and the effect of the composition group of the present invention is better than that of the experimental AD group, indicating that the composition of the present invention can effectively reduce the oxidative stress level of mice with acute liver injury induced by LPS, thereby protecting liver damage.

[0050] Test Example 2 Evaluation of the effect of different compositions on relieving physical fatigue 210 SPF-grade ICR mice aged 4-5 weeks (weighing 18-22 g) were selected and, after adaptive feeding for 1 week, randomly divided into 11 groups, namely, a blank group, Example 1-3 groups (20 mg / kg), and an experiment AE group (20 mg / kg), with 10 mice in each group. 0.2 g / 10 g was administered by gavage for 30 consecutive days, and an equal volume of normal saline was given to the blank control group.

[0051] (1) Effects of different compositions on weight-bearing swimming time of mice 30 minutes after the last oral administration of the test substance, mice with a lead weight of 5% of their body weight at the base of their tails were placed in a swimming box with a water depth of 40 cm and a water temperature of 25±1.0℃. The time from the start of swimming to the death of the mice, i.e., the weighted swimming time of the mice, was recorded. The measurement results are shown in Table 4.

[0052] Table 4 Effects of different compositions on weight-bearing swimming time of mice

[0053] As can be seen from Table 4, compared with the blank group, the weighted swimming time of mice in Example 1-3 groups and Test Example AC groups was prolonged, and the effect of extending the weighted swimming time of mice in Example 2 was the best; Test Groups A and B removed some raw materials, and although the weighted swimming time of mice was prolonged after administration, it was significantly lower than that in Example 1-3 groups, indicating that the removed raw materials played a major role in prolonging the weighted swimming time of mice; Compared with Test Example CD Group, it removed the black fruit glandular rib aronia fruit extract and Hypericum, and the extension time of the weighted swimming of mice was significantly reduced compared with Example 2, which shows that there is a synergistic effect between the black fruit glandular rib aronia fruit extract and Hypericum, and the synergistic effect can significantly increase the weighted swimming time of mice and increase the exercise endurance of mice; Test Group E is a composition in the prior art, although it can also prolong the weighted swimming time of mice, but the degree of extension is significantly lower than that of the composition of the present invention.

[0054] (2) Effects of different compositions on serum urea nitrogen levels in mice after exercise 30 minutes after the last administration of the test substance, the mice were placed in 30℃ water to swim for 90 minutes, and rested for 60 minutes after exercise. 0.5mL of eye blood was collected; after being placed in a 4℃ refrigerator for 3 hours, the serum was separated by centrifugation at 1500rpm for 15 minutes, and urea nitrogen was determined using a kit (urease method). The results are shown in Table 5.

[0055] Table 5 Effects of different compositions on serum urea nitrogen levels in mice after exercise

[0056] Serum urea nitrogen is a metabolite of protein. During strong exercise, protein metabolism is accelerated, serum BUN increases significantly, and the clearance rate of BUN can reflect the body's ability to resist fatigue. As shown in Table 5, compared with the blank group, the serum urea nitrogen levels of Example 1-3 and Test Example AC groups were slightly reduced; after removing some raw materials, the AD group reduced the urea nitrogen level in the mouse serum after administration, but it was significantly higher than the Example 1-3 group, which shows that there is a synergistic effect between the compositions of the present invention, which can reduce the serum urea nitrogen of mice after exercise, help fatigue recovery and reduce exercise load; Test E group is a composition in the prior art, which can also reduce the serum urea nitrogen level, but the degree of reduction is significantly lower than the composition of the present invention.

[0057] (3) Effects of different compositions on blood lactate levels in mice before and after exercise 30 minutes after the last administration of the test substance, 20 μL of eye blood was collected and then placed in water at 30°C for swimming for 10 minutes. 20 μL of blood was collected again at 0 minute and 20 minutes after exercise, and lactate was measured using a biochemical analyzer, where: the area under the blood lactate curve = 1 / 2×(blood lactate value before swimming + blood lactate value 0 minute after swimming)×10+1 / 2×(blood lactate value 0 minute after swimming + blood lactate value 20 minutes after rest after swimming)×20. The measurement results are shown in Table 6.

[0058] Table 6 Effects of different compositions on blood lactate levels in mice before and after exercise

[0059] During strenuous exercise, the body's metabolic product lactic acid is one of the causes of fatigue, and the blood lactate level can reflect aerobic metabolic capacity, the generation and elimination speed of fatigue. As shown in Table 6, compared with the blank group, the area under the blood lactate curve of Example 1-3 group and Test Example AE group was significantly reduced; after removing some raw materials from the test AD group, although the blood lactate level and the area under the blood lactate curve of mice after exercise were reduced after administration, they were significantly higher than those of Example 1-3 group, which shows that there is a synergistic effect between the compositions of the present invention, which can significantly reduce the blood lactate level of mice after exercise and improve physical fatigue caused by exercise; Test E group is a composition in the prior art, which can also reduce the blood lactate level of mice after exercise, but the degree of reduction is significantly lower than that of the composition of the present invention.

[0060] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0061] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A ginseng and cistanche composite for relieving fatigue and protecting liver damage, comprising the following raw materials: ginseng, cistanche, oyster, kudzu root, turmeric, schisandra chinensis, aronia nigra, hypericum perforatum, cassia seed, glutathione-rich yeast extract, taurine and oligosaccharides; Preferably, the composition further comprises a fragrance; the fragrance is alanine; Preferably, the oligosaccharide is one or more of fructooligosaccharide, xylooligosaccharide, isomaltooligosaccharide and galactooligosaccharide; Preferably, the composition is used for relieving fatigue and protecting liver damage.

2. The composition according to claim 1, characterized in that The raw materials included are, by weight: 45-60 parts of ginseng, 40-50 parts of cistanche deserticola, 35-50 parts of oyster, 35-45 parts of kudzu root, 30-42 parts of turmeric, 28-38 parts of schisandra chinensis, 25-31 parts of aronia nigra, 20-30 parts of hypericum perforatum, 18-26 parts of cassia seed, 0.01-0.5 parts of glutathione-rich yeast extract, 0.01-0.3 parts of spices, 0.01-0.3 parts of taurine and 5-13 parts of oligosaccharides; And / or, preferably, the composition comprises the following raw materials in parts by weight: 52 parts of ginseng, 50 parts of Cistanche deserticola, 35 parts of oysters, 40 parts of Pueraria root, 42 parts of turmeric, 33 parts of Schisandra chinensis, 28 parts of Aronia nigra fruits, 20 parts of Hypericum perforatum, 22 parts of Cassia seed, 0.24 parts of glutathione-rich yeast extract, 0.3 parts of spices, 0.01 parts of taurine and 5 parts of oligosaccharides.

3. The use of the composition according to any one of claims 1 to 2, characterized in that: The use is to prepare medicines, health products or foods for relieving fatigue and protecting liver damage.

4. A pharmaceutical preparation for relieving fatigue and protecting liver damage, characterized in that: The preparation comprises the composition according to any one of claims 1 to 2 and a pharmaceutically acceptable carrier.

5. The pharmaceutical preparation according to claim 4, characterized in that First, extract schisandra chinensis to obtain schisandra chinensis alcohol extract, and then prepare a pharmaceutical preparation; the preparation method of the schisandra chinensis alcohol extract is: dry and crush the schisandra chinensis, pass it through a 40-60 mesh sieve, add the schisandra chinensis powder into an ethanol aqueous solution according to the ratio of schisandra chinensis powder: ethanol m:v (g / mL) = 1:8-12, soak it, reflux extraction, combine the extracts and centrifuge them, and dry them under reduced pressure to obtain the schisandra chinensis alcohol extract; Preferably, the volume concentration of the ethanol aqueous solution is 70%-90%; the soaking time is 20-40 min; the number of extractions is 2-4 times, and the extraction time is 1-2 h / time; the centrifugation is 1000-1400 rpm for 10-20 min.

6. The pharmaceutical preparation according to claim 4, characterized in that First, extract the Aronia nigra fruit to obtain Aronia nigra fruit alcohol extract, and then prepare a pharmaceutical preparation; the preparation method of the Aronia nigra fruit alcohol extract is: after drying the Aronia nigra, crush it, pass it through a 60-80 mesh sieve, add the Aronia nigra to an ethanol aqueous solution according to the ratio of Aronia nigra: ethanol m:v (g / mL) = 1:20-30, stir evenly, extract by water bath ultrasonication, filter, centrifuge, and dry under reduced pressure to obtain the Aronia nigra fruit extract; Preferably, the volume concentration of the ethanol aqueous solution is 70%-80%; the water bath temperature is 60-70°C, the ultrasonic power is 50-100W, the extraction time is 2-4h; and the centrifugation is 600-800rpm for 10-16min.

7. The method for preparing the composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: (1) Weigh the raw materials according to the above weight proportions and set aside; (2) Ginseng, Cistanche deserticola, and oyster are dried and crushed, and passed through a 60-80 mesh sieve to obtain a mixed powder A. The mixed powder A is added to an ethanol aqueous solution at a ratio of mixed powder A: ethanol m:v (g / mL) = 1:10-14, and the mixed powder A is soaked, refluxed for extraction, and the extracts are combined and centrifuged. After reduced pressure drying, extract I is obtained; (3) Drying and crushing Pueraria root, turmeric, Hypericum perforatum and Cassia seed, and passing through a 40-60 mesh sieve to obtain a mixed powder B, adding the mixed powder B to n-butanol at a ratio of mixed powder B: n-butanol m:v (g / mL) = 1:10-16, stirring evenly, performing ultrahigh pressure assisted extraction, filtering, centrifuging and drying under reduced pressure to obtain extract II; (4) Extract I and extract II are mixed, and Schisandra chinensis extract, Aronia nigra fruit extract, glutathione-rich yeast extract, spices, taurine and oligosaccharides are added in sequence, mixed and homogenized, and dried under reduced pressure to obtain a composition.

8. The preparation method according to claim 7, characterized in that: In step (2), the volume concentration of the ethanol aqueous solution is 50%-60%; the soaking time is 10-20 minutes; the extraction times are 3-5 times, and the extraction time is 1-2 hours; the centrifugation is 550-650 rpm for 5-10 minutes.

9. The preparation method according to claim 7, characterized in that: In step (3), the extraction pressure is 90-110 MPa, the extraction time is 3-7 min, the centrifugation is 800-1200 rpm for 8-12 min, and the reduced pressure drying is performed to a water content of 5-9%.

10. The preparation method according to claim 7, characterized in that: The homogenizing speed is 1200-1600 rpm, and the homogenizing time is 10-18 min.

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