Fiber dressing and preparation method thereof
By combining silver-containing antibacterial fibers and NO donor fibers in the fiber dressing, the problems of poor antibacterial effect and slow wound healing are solved, and better antibacterial performance and wound healing are achieved.
Patent Information
- Application Number
- CN202411485820.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-23
- Publication Date
- 2025-02-28
AI Technical Summary
The existing fiber dressings have poor antibacterial effects and slow wound healing, which cannot effectively solve the external and self-adverse factors faced by chronic wounds during the healing process.
Non-woven fabrics were prepared using silver-containing antibacterial fibers and NO donor fibers with mass ratios of (1-20): 1, and silver compounds and NO donors were attached to the two fibers, respectively, to reduce mutual interference, enhance antibacterial properties, and promote wound healing through NO donors.
It achieves synergistic enhancement of antibacterial properties and promotes wound healing. The fiber dressing has good stability and effect, which is significantly better than commercially available products.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of medical supplies, and in particular to a fiber dressing and a preparation method thereof. Background Art
[0002] Chronic wounds are subject to various external and internal factors during the healing process, which can lead to slow wound healing or even long-term inability to heal. These adverse factors include: excessive exudate soaking into the wound, microbial infection, inflammation and oxidative stress on the wound, slow vascularization, etc. The performance of existing dressing products on the market is relatively single and cannot solve the various internal and external adverse factors faced by chronic wounds.
[0003] Existing technologies usually focus on adsorbing or attaching metal ions with antibacterial functions to dressings, and mainly pay attention to the concentration and antibacterial effect of antibacterial agents, but often ignore the requirements of regulating wound inflammation, anti-oxidation and accelerating vascularization; and in the case of limited functions of metal antibacterial agents, existing technologies usually focus on mechanical stacking of antibacterial substances, the antibacterial properties of antibacterial substances cannot be fully exerted, mutual interference may occur, the overall antibacterial effect is reduced, and even drug resistance and cytotoxicity may be increased, resulting in a longer wound healing time. Summary of the invention
[0004] In order to solve the problems of unsatisfactory antibacterial effect and slow wound healing of existing fiber dressings, the present invention provides a fiber dressing and a preparation method thereof.
[0005] In a first aspect, the present application provides a fiber dressing: A fiber dressing, wherein the raw materials include silver-containing antibacterial fiber and NO donor fiber in a mass ratio of (1-20):1, wherein the fiber dressing is a non-woven fabric prepared from the raw materials of the fiber dressing; the silver nitrite in the silver-containing antibacterial fiber accounts for 0.01wt%-10wt% of the silver-containing antibacterial fiber; the NO donor substance in the NO donor fiber accounts for 0.001wt%-10wt% of the NO donor fiber; and the NO donor is one or more of arginine, nitrate esters, azodiol ene salts, S-nitrosothiol, and furazan nitrogen oxide.
[0006] The present invention prepares silver-containing fibers and NO donor fibers respectively, then uses the silver-containing fibers and NO donor fibers to prepare non-woven fabrics, and attaches silver compounds and NO donors to the two fibers respectively, thereby reducing the mutual influence of the two fibers in preparation process and performance.
[0007] Compared with loading silver-containing compounds and NO donors on the same fiber at the same time, preparing silver-containing fibers and NO donors separately can reduce the interference of silver ions on NO donors. For example, silver ions can form coordination compounds with arginine, thereby interfering with the release of NO from arginine and the antibacterial effect of silver antibacterial agents. In addition, silver ions as transition metals can promote the decomposition of azodienol salts, causing azodienol salts to decompose into NO during storage. Therefore, attaching silver compounds and NO donors to two types of fibers can not only reduce mutual interference, but also synergistically enhance antibacterial properties. At the same time, the NO released by the NO donor promotes wound healing. The two work together to exert the best effect, and the prepared fiber dressing has good stability.
[0008] NO donors can participate in the storage and transport of NO, and release NO under specific conditions of the wound to participate in physiological processes, provide antibacterial effects, and promote the growth of new cells and / or blood vessels in the wound.
[0009] L-arginine can be converted into L-citrulline and NO by the action of three isozymes in the human body. The three isozymes are neuronal NO synthase (nNOS), endothelial NO synthase (eNOS) and inducible NO synthase (iNOS).
[0010] Neuronal NO synthase (nNOS) is mainly present in neurons and skeletal muscles; endothelial NO synthase (eNOS) is mainly present in endothelial cells and epithelial cells, and sometimes also in some neuronal cells; inducible NO synthase (iNOS) is widely distributed in the human body, especially in macrophages, hepatocytes, astrocytes and smooth muscle cells.
[0011] Under physiological conditions, one molecule of azodiol ene salt constructed with nitrogen bonds can be hydrolyzed to generate two molecules of NO, and the release of NO does not require unique metabolism or redox reactions. Therefore, this type of NO donor can be conveniently used in biomedical research.
[0012] Nitrosothiols can not only store NO, but also transport NO in organisms. Nitrosothiols have good biocompatibility. Nitrosothiols such as glutathione thiol, albumin thiol, cysteine thiol, etc. can release NO through the stimulation of transition metals (such as Cu+), ascorbic acid, ultraviolet light, heat, or enzymes (superoxide dismutase and protein disulfide isomerase).
[0013] Furthermore, the fibers in the silver-containing antibacterial fiber and the NO donor fiber are one of CMC fiber, seaweed fiber, PVA fiber, and viscose fiber; the silver compound in the silver-containing antibacterial fiber is one of silver nitrite, silver chloride, and silver sulfate.
[0014] Silver nitrite is produced by the reaction of silver nitrate and nitrite (nitrites include sodium nitrite and potassium nitrite); silver chloride is produced by the reaction of silver nitrate and chlorides, which include sodium chloride, potassium chloride and calcium chloride; silver sulfate is produced by the reaction of silver nitrate and sulfate (sulfates include sodium sulfate and sulfuric acid).
[0015] Furthermore, the fiber has a length of 38-52 mm and a linear density of 1.2-2 dtex.
[0016] Furthermore, the raw materials of the fiber dressing also include reinforcing fibers, and the reinforcing fibers are ES fibers or nylon fibers; the amount of the reinforcing fibers is 1-20 wt% of the total amount of the silver-containing antibacterial fibers and the NO donor fibers.
[0017] The reinforcing fibers can improve the strength of the hydrophilic fiber needle-punched cloth, i.e., the fiber dressing, to prevent the product from breaking when it is inserted into the human body cavity, thereby making it difficult to remove.
[0018] Furthermore, the fiber dressing has a thickness of 1.5-2.2 mm.
[0019] In a second aspect, the present application provides a method for preparing a fiber dressing: A method for preparing a fiber dressing comprises the following preparation steps: The silver-containing antibacterial fiber is prepared by sequentially impregnating the fiber with a 0.1-50wt% silver nitrate solution and a 0.1-50wt% salt solution, wherein the salt solution is one of nitrite, chloride and sulfate; The NO donor fiber is prepared by impregnating the fiber with 0.1-50wt% NO donor solution; The fiber dressing is prepared by laying the silver-containing antibacterial fiber and the NO donor fiber into a fiber net, and then hot pressing or acupuncture is performed to prepare the fiber dressing.
[0020] Furthermore, the mass fraction of silver nitrate in the silver nitrate solution is 0.1-30wt%; the mass fraction of salt in the salt solution is 0.1-25wt%; and the mass fraction of NO donor in the NO donor solution is 0.1-30wt%.
[0021] Furthermore, the mass fraction of silver nitrate in the silver nitrate solution is 0.1-20%; the mass fraction of salt in the salt solution is 0.1-20%; and the mass fraction of NO donor in the NO donor solution is 0.1-10%.
[0022] Furthermore, the mass fraction of silver nitrate in the silver nitrate solution is 0.5-5wt%; the mass fraction of salt in the salt solution is 0.1-0.5wt%; and the mass fraction of NO donor in the NO donor solution is 0.5-2wt%.
[0023] Furthermore, the nitrite is sodium nitrite or potassium nitrite, the chloride is sodium chloride, potassium chloride or calcium chloride, and the sulfate is sodium sulfate, potassium sulfate or ammonium sulfate.
[0024] Furthermore, the preparation of the silver-containing antibacterial fiber includes the following preparation steps: Put 1 part of the fiber into 1-4 parts of silver nitrate solution, stir and soak for 6-8 hours, remove the solvent, and prepare silver nitrate fiber; Then, the silver nitrate fiber is immersed in a salt solution, stirred and immersed for 0.5-5 hours to prepare a silver-containing antibacterial fiber, and then rinsed and dried for standby use; The preparation of the NO donor fiber comprises the following preparation steps: The fiber is immersed in a NO donor solution for 1-10 hours to prepare a NO donor fiber, and then the solvent is removed for standby use; the mass ratio of the fiber to the NO donor solution is 1:3-1:20.
[0025] Furthermore, the temperature of the NO donor solution is 20-60°C.
[0026] Furthermore, the solvents in the silver nitrate solution, the nitrite solution and the NO donor solution are at least one of ethanol, acetone and water. Beneficial Effects
[0027] 1. A fiber dressing, wherein the raw materials include silver-containing antibacterial fiber and NO donor fiber in a mass ratio of (1-4):1, wherein the fiber dressing is a non-woven fabric prepared from the raw materials of the fiber dressing; the silver compound in the silver-containing antibacterial fiber is 0.01wt%-10wt% of the silver-containing antibacterial fiber; the NO donor substance in the NO donor fiber is 0.001wt%-10wt% of the NO donor fiber; the NO donor is one or more of arginine, nitrate esters, azodiol ene salts, S-nitrosothiol, and furazan nitrogen oxide; the present invention attaches the silver compound and the NO donor to two types of fibers respectively, thereby reducing the mutual influence of the two types of fibers in preparation process and performance, synergistically enhancing the antibacterial performance, and at the same time, the NO released by the NO donor promotes wound healing, and the two synergistically exert the best effect, so that the prepared fiber dressing has good stability. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 , the morphological diagram of Escherichia coli and Candida albicans in the antibacterial ring test results of the fiber dressing of Example 1; Figure 2 , the morphological diagram of Escherichia coli and Candida albicans in the antibacterial ring test results of the fiber dressing of Example 2; Figure 3, the morphological diagram of Escherichia coli and Candida albicans in the antibacterial ring test results of the fiber dressing of comparative example 1; Figure 4 , morphological diagram of Escherichia coli and Candida albicans in the inhibition ring test results of commercially available dressings.
[0029] Reference numerals: a: Escherichia coli; b: Candida albicans. DETAILED DESCRIPTION
[0030] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments.
[0031] Example 1, a fiber dressing The silver-containing antibacterial fiber is silver nitrite antibacterial fiber, and its preparation process is as follows: 1) Dissolve 1 kg of silver nitrate in 99 kg of solvent (the solvent is a mixture of ethanol and water in a mass ratio of 1:1) to obtain a 1 wt% silver nitrate solution.
[0032] 2) 62.5 kg of CMC fibers with a length of 38 mm and a linear density of 1.2 dtex were placed into the silver nitrate ethanol aqueous solution in step 1 and stirred and soaked for 8 hours.
[0033] 3) The CMC fiber in step 2 is taken out and dehydrated to obtain silver nitrate fiber.
[0034] 4) Soak the silver nitrate fiber in a sodium nitrite solution in step 3 for 2 hours to obtain a silver nitrite antibacterial fiber with a silver nitrite content of 1.3wt%; the ratio of the sodium nitrite solution is 0.4kg sodium nitrite, 40kg water, and 200kg ethanol (the mass fraction of sodium nitrite in the sodium nitrite solution is 0.17wt%).
[0035] 5) Take out the silver nitrite antibacterial fiber from step 4, add ethanol aqueous solution, and rinse several times.
[0036] 6) Drain the solvent and dry the fiber at low temperature for later use.
[0037] Preparation of NO donor-containing fibers: 1) Dissolve 1 kg of arginine in 99 kg of water, stir evenly to fully dissolve, and prepare an arginine solution, which is the NO donor solution.
[0038] 2) Soak 15.6 kg of viscose fiber in ethanol at room temperature for 8 hours, stirring occasionally.
[0039] 3) The viscose fibers are subjected to high-speed centrifugation to remove ethanol.
[0040] 4) The viscose fiber treated in step 3) is placed into the NO donor solution prepared in step 1 and slowly stirred for 5 hours. The temperature of the NO donor solution is 45°C.
[0041] 5) The fibers in step 4 are centrifuged to remove moisture to obtain NO donor fibers, specifically arginine fibers.
[0042] 6) The cellulose containing arginine obtained in step 5 is dried at low temperature to obtain NO donor fiber with an arginine content of 0.6 wt %.
[0043] Preparation of finished dressing: 1) CMC fiber containing silver nitrite and arginine fiber were mixed evenly in a mass ratio of 4:1 and then needle-punched into a needle-punched non-woven fabric with a thickness of 2.0 mm.
[0044] 2) Die-cut the needle-punched nonwoven fabric into the required size and then package and sterilize it.
[0045] Example 2, a fiber dressing The silver-containing antibacterial fiber is silver nitrite seaweed fiber, and its preparation process is as follows: 1) Dissolve 1 kg of silver nitrate in 99 kg of ethanol-water solution (the mass ratio of ethanol to water is 1:1) to obtain a 1 wt% silver nitrate solution.
[0046] 2) 50 kg of seaweed fiber with a linear density of 2.0 dtex and a length of 52 mm was immersed in the silver nitrate solution obtained in step 1 at room temperature for 6 hours to obtain silver nitrate fiber.
[0047] 3) Take out the silver nitrate fiber from step 2) and dehydrate it.
[0048] 4) Soaking the silver nitrate fiber obtained in step 3) in a sodium nitrite solution for 4 hours to obtain a silver nitrite seaweed fiber with a silver nitrite content of 1.2wt%; the ratio of the sodium nitrite solution is 0.4kg sodium nitrite, 20kg water, and 100kg ethanol (the mass concentration of sodium nitrite in the sodium nitrite solution is 0.33wt%).
[0049] 5) Take out the silver nitrite alginate fiber from step 4), add ethanol aqueous solution, rinse several times and centrifuge to remove the liquid.
[0050] 6) Dry the fiber from step 5 at room temperature and set aside.
[0051] Preparation of NO donor fibers 5 kg of CMC fiber with a length of 52 mm and a linear density of 2.0 dtex was taken and immersed in 10 kg of an acetone solution of 2 wt% isosorbide mononitrate at room temperature for 10 hours to prepare a NO donor fiber containing 1 wt% isosorbide mononitrate. The solvent was drained and the fiber was dried at room temperature for later use.
[0052] Preparation of finished dressings Silver nitrite seaweed fiber and NO donor fiber were mixed in a mass ratio of 10:1, and then prepared by a needle punching process to form a needle-punched non-woven fabric with a thickness of 2.0 mm to obtain a fiber dressing, which was die-cut, packaged, sterilized and set aside.
[0053] Example 3, a fiber dressing The difference from the embodiment is that the raw materials of the fiber dressing also include reinforcing fibers, the reinforcing fibers are nylon fibers (density is 1.15 g / cm³), and the amount of the reinforcing fibers is 15 wt% of the total amount of the silver-containing antibacterial fibers and the NO donor fibers.
[0054] Comparative Example 1, a fiber dressing The silver-containing antibacterial fiber is silver nitrite seaweed fiber, and its preparation process is as follows: 1) Take 1 kg of arginine and dissolve it in 99 kg of solvent (the solvent is a mixture of ethanol and water in a mass ratio of 1:1), stir evenly and fully dissolve it to prepare an arginine solution.
[0055] 2) Take 62.5 kg of CMC fiber with a length of 38 mm and a linear density of 1.2 dtex and soak it in ethanol for 8 hours, stirring it from time to time.
[0056] 3) The CMC fibers in step 2 are centrifuged at high speed to remove liquid.
[0057] 4) The CMC fiber treated in step 3 is placed in the arginine solution prepared in step 1 and slowly stirred for 5 hours at 45° C. to obtain NO donor fiber.
[0058] 5) The NO donor fiber in step 4 is centrifuged to remove moisture.
[0059] 6) Dissolve 1 kg of silver nitrate in 99 kg of solvent to form a silver nitrate solution. The solvent is ethanol and water in a mass ratio of 1:1.
[0060] 7) The NO donor fiber of step 5 is placed in the silver nitrate solution of step 6 and immersed for 2 hours to obtain NO donor / silver nitrate fiber.
[0061] 8) taking out the NO donor / silver nitrate fiber from step 7 and dehydrating it; 9) Soak the NO donor / silver nitrite fiber prepared in step 8 in a sodium nitrite solution (the ratio of the sodium nitrite solution is 0.4 kg sodium nitrite, 5 kg water, and 20 kg ethanol) for 5 hours to obtain a NO donor / silver nitrite fiber.
[0062] 10) Take out the NO donor / silver nitrite fiber from step 9, add ethanol aqueous solution, and rinse several times; 11) draining the solvent and drying the fibers at low temperature; preparing the mixed fibers through a needle punching process to form a needle punched non-woven fabric with a thickness of 2.0 mm to obtain a fiber dressing, which is die-cut, packaged, sterilized, and set aside.
[0063] Performance Testing 1. Antibacterial test The samples of Example 1 and Example 2 were taken, and the common commercially available hydrophilic fiber silver dressing was tested for antibacterial ring test. The circular samples with a diameter of 10 mm and the same thickness were taken to test the antibacterial effects on Escherichia coli and Candida albicans respectively. The center of each culture dish was a common non-woven blank control group, and the four circles around it were test samples. The antibacterial ring test results are shown in FIG. Figures 1 to 4 And Table 1.
[0064] from Figures 1 to 4 It can be seen that there are obvious antibacterial rings around the samples of Example 1 and Example 2, while the antibacterial rings of Example 3 and the control product are closely attached to the samples. Therefore, the antibacterial effects of Example 1 and Example 2 are significantly better than those of Example 3 and the control product.
[0065] Table 1. Antibacterial ring data of the fiber dressings of Example 1, Example 2 and Comparative Example 1 and commercially available fiber dressings distinguish Diameter of Escherichia coli inhibition ring (mm) Diameter of Candida albicans inhibition zone (mm) Example 1 13 13 13 13 12 12 12 13 Example 2 13 13 13 12 12 12 12 12 Example 3 13 13 13 13 12 12 12 13 Comparative Example 1 10 10 10 10 10 10 10 10 A hydrophilic fiber silver dressing on the market (CMC fiber loaded with 1.2wt% silver) 10 10 10 10 10 10 10 10 2. Wound healing days Take 4 Bama pigs, establish a full-thickness skin defect wound animal model, use the sample of Example 1 (test group), comparative example 1 (control group 1), the above similar product dressing (control group 2) and sterile gauze covering (blank group), and fix them. Change the product every day in the first 7 days. If there is no tissue exudate in the later period, change the product every week. Observe the wound infection regularly every day until the wound heals (the wound is judged to be healed if there is no exudate and redness). Create 4 full-thickness skin defect wounds with the same conditions on the right side of the back of each Bama pig (the 4 wounds on the same side are the first wound, the second wound, the third wound and the fourth wound from front to back), and cover the wounds of each animal with the sample of Example 1 (test group), comparative example 1 (control group 1), the above similar product dressing (control group 2) and sterile gauze covering (blank group). Each test sample can correspond to the first wound, the second wound, the third wound and the fourth wound on the 4 Bama pigs. The number of days required for healing of each experimental sample is averaged (the other conditions of the Bama pigs during the experiment are the same), and the experimental results are as follows.
[0066] distinguish Exudate on day 7 Exudate status on day 14 Exudate status on day 21 Average number of days for wound healing Example 1 Moderate purulent A small amount of purulent / 15 Control group 1 A lot of purulent A lot of purulent Moderate purulent 28 Control group 2 A lot of purulent A lot of purulent Moderate purulent 25 Blank Group A lot of purulent A lot of purulent A lot of purulent No healing after 35 days
[0067] The results showed that the experimental group had the best effect in wound antibacterial effect and promotion of wound healing, the control group 1 and the control group 2 had equivalent effects, and the blank group had the worst effect.
[0068] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.
Claims
1. A fiber dressing, characterized in that: The raw materials include silver-containing antibacterial fiber and NO donor fiber in a mass ratio of (1-20):1; the fiber dressing is a non-woven fabric prepared from the raw materials of the fiber dressing; the silver compound in the silver-containing antibacterial fiber is 0.01wt%-10wt% of the silver-containing antibacterial fiber; the content of NO donor substance in the NO donor fiber is 0.001wt%-10wt%; the NO donor is one or more of arginine, nitrates, azodiol ene salts, S-nitrosothiol, and furazan nitrogen oxides.
2. The fiber dressing according to claim 1, characterized in that: The fibers in the silver-containing antibacterial fiber and the NO donor fiber are one of CMC fiber, seaweed fiber, PVA fiber and viscose fiber; the silver compound in the silver-containing antibacterial fiber is one of silver nitrite, silver chloride and silver sulfate.
3. The fiber dressing according to claim 2, characterized in that: The fiber has a length of 38-52 mm and a linear density of 1.2-2 dtex.
4. The fiber dressing according to claim 1, characterized in that: The raw materials of the fiber dressing also include reinforcing fibers, which are ES fibers or nylon fibers. The amount of the reinforcing fibers is 0.1-20 wt% of the total amount of the silver-containing antibacterial fibers and the NO donor fibers.
5. The fiber dressing according to claim 1, characterized in that: The thickness of the fiber dressing is 1.5-2.2 mm.
6. A method for preparing the fiber dressing according to any one of claims 1 to 5, characterized in that: The method comprises the following preparation steps: The silver-containing antibacterial fiber is prepared by sequentially impregnating the fiber with a 0.1-50wt% silver nitrate solution and a 0.1-50wt% salt solution, wherein the salt solution is one of nitrite, chloride and sulfate; The NO donor fiber is prepared by impregnating the fiber with 0.1-50wt% NO donor solution; The fiber dressing is prepared by laying the silver-containing antibacterial fiber and the NO donor fiber into a fiber net, and then hot pressing or acupuncture is performed to prepare the fiber dressing.
7. The method for preparing the fiber dressing according to claim 6, characterized in that: The nitrite is sodium nitrite or potassium nitrite, the chloride is sodium chloride, potassium chloride or calcium chloride, and the sulfate is sodium sulfate, potassium sulfate or ammonium sulfate.
8. The method for preparing the fiber dressing according to claim 6, characterized in that: The preparation of the silver-containing antibacterial fiber includes the following preparation steps: Put 1 part of fiber into 1-4 parts of silver nitrate solution, stir and soak for 6-8 hours, remove the solution, and prepare silver nitrate fiber; Then, the silver nitrate fiber is immersed in a salt solution, stirred and immersed for 0.5-5 hours to prepare a silver-containing antibacterial fiber, and then rinsed and dried for standby use; The preparation of the NO donor fiber comprises the following preparation steps: The fiber is immersed in a NO donor solution for 1-10 hours to prepare a NO donor fiber, and then the solvent is removed for standby use; the mass ratio of the fiber to the NO donor solution is 1:3-1:
20.
9. The method for preparing the fiber dressing according to claim 8, characterized in that: The temperature of the NO donor solution is 20-60°C.
10. The method for preparing the fiber dressing according to any one of claims 7 to 8, characterized in that: The solvents in the silver nitrate solution, the nitrite solution and the NO donor solution are at least one of ethanol, acetone and water.