Method for increasing ceramide content in fermented tea seed oil and application of method in skin care products

Abstract

The invention relates to a method for increasing the content of ceramide in fermented tea-seed oil and application of the ceramide in skin care products, and the method comprises the following steps: taking a mixture containing tea-seed oil and a culture medium, and carrying out sterilization treatment to obtain an initial fermentation system; inoculating bumblebee candida into the initial fermentation system, carrying out fermentation culture until first culture time is reached, and adding a carbon source; continuously carrying out fermentation culture until the final culture time is reached, so as to obtain fermentation liquor; the fermentation broth is subjected to layering, centrifugation, dehydration and deodorization treatment after being inactivated so as to obtain the fermented tea seed oil, and the preservation number of the candida bumblebee is CGMCC No.25797. The fermented tea seed oil prepared by the technical scheme provided by the invention has more excellent barrier repairing and skin stability maintaining functions on human skin cells, and also has more remarkable effects in the aspects of relieving, anti-inflammation, moisturizing, anti-oxidation and the like.

Description

Technical Field

[0001] The present application relates to the field of cosmetic technology, and in particular to a method for increasing the ceramide content in fermented tea seed oil and its application in skin care products. Background Art

[0002] Tea seed oil is a high-end woody health oil squeezed from the kernels wrapped in cattails in tea seeds. Tea seeds contain a large number of valuable nutrients, including tea polyphenols, VE, linoleic acid and other nutrients needed by the human body. Tea seed oil made from tea seeds is a nutritious edible oil with great health conditioning effects. In addition, tea seed oil is an edible oil naturally rich in tea polyphenols, and has good anti-aging, anti-radiation, and beauty and skin care effects.

[0003] Ceramide is a type of amide compound formed by dehydration of long-chain fatty acids and the amino group of sphingosine. 40% to 50% of sebum in the stratum corneum is composed of ceramide. Ceramide is the main part of the intercellular matrix and plays an important role in maintaining the balance of moisture in the stratum corneum. Therefore, ceramide plays an extremely important role in skin care.

[0004] Tea seed oil is rich in ceramides, but due to the wide variety of ceramides, the complex production and metabolism of microorganisms, and the lipophilic nature of the ceramides, the extraction rate of ceramides in tea seed oil is not high, the content is low, and its production and application are limited. Summary of the invention

[0005] In view of the problems existing in the prior art, the embodiments of the present application provide a fermented tea seed oil and a preparation method, application, and cosmetics thereof, in order to increase the content of ceramide in the fermented tea seed oil.

[0006] In a first aspect, the present invention provides a method for increasing the ceramide content in fermented tea seed oil, comprising the following steps:

[0007] Taking a mixture containing tea seed oil and culture medium and sterilizing it to obtain an initial fermentation system;

[0008] Inoculating Candida bombus into the initial fermentation system for fermentation and culturing until a first culturing time is reached, and adding a carbon source;

[0009] Continuing the fermentation culture until the final culture time is reached to obtain a fermentation liquid;

[0010] After the fermentation liquid is inactivated, it is subjected to layering, centrifugation, dehydration and deodorization treatment to obtain fermented tea seed oil; wherein,

[0011] The deposit number of the bumblebee Candida is CGMCC No.25797.

[0012] In some embodiments, based on the volume of the culture medium, the inoculation volume of the Candida bombus is 5% to 10% of the volume of the culture medium.

[0013] In some embodiments, the culture medium includes yeast extract, peptone, soy protein, monopotassium phosphate, dipotassium phosphate, ammonium sulfate, glycerol, and water.

[0014] In some embodiments, based on the total mass of the culture medium, the mass content of potassium dihydrogen phosphate is 3% to 7%, and the added amount of dipotassium hydrogen phosphate is 10% to 20%.

[0015] In some embodiments, based on the volume of the culture medium, the added amount of the tea seed oil is 300-600 g / L.

[0016] In some embodiments, the carbon source comprises one or more of molasses, glucose, sucrose, and soluble starch.

[0017] In some embodiments, the carbon source is added in an amount of 10 g / L to 15 g / L based on the volume of the culture medium.

[0018] In some embodiments, the continuing the fermentation culture until the final culture time is reached to obtain the fermentation broth comprises:

[0019] The fermentation system with the added carbon source is continuously cultured until the second culture time is reached, and serine is added;

[0020] Continuing the fermentation culture until the final culture time is reached to obtain a fermentation liquid;

[0021] Optionally, based on the volume of the culture medium, the amount of serine added is 1 g / L to 5 g / L.

[0022] In some embodiments, the first culture time is 24 h to 36 h.

[0023] In some embodiments, the second culture time is 42 h to 60 h.

[0024] In some embodiments, the final culture time is 96 h to 120 h.

[0025] In a second aspect, an embodiment of the present application provides a tea seed oil prepared by the method of an embodiment of the first aspect of the present application.

[0026] In a third aspect, the embodiments of the present application provide the use of tea seed oil prepared according to the method of the embodiments of the first aspect of the present application or the tea seed oil according to the embodiments of the second aspect of the present application in skin care products.

[0027] In a fourth aspect, an embodiment of the present application provides a skin care product, comprising tea seed oil prepared according to the method of the embodiment of the first aspect of the present application or tea seed oil prepared according to the embodiment of the second aspect of the present application.

[0028] In some embodiments, the mass content of fermented tea seed oil in the skin care product is 0.1% to 80%. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solution of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0030] Figure 1 HPLC spectrum of ceramide.

[0031] Figure 2 This is a comparison chart of the ceramide content in the fermented tea seed oil of some embodiments of the present application (in the figure, * indicates significant difference (P value), * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and # indicates significant difference analysis between fermented tea seed oil and unfermented tea seed oil).

[0032] Figure 3 This is a comparison chart of oil-water sedimentation separation in the fermentation liquid in some embodiments of this application. DETAILED DESCRIPTION

[0033] The features and exemplary embodiments of various aspects of the present application will be described in detail below. In the detailed description below, many specific details are proposed to provide a comprehensive understanding of the present application. However, it is obvious to those skilled in the art that the present application can be implemented without the need for some of these specific details. The following description of the embodiments is only to provide a better understanding of the present application by illustrating the examples of the present application. In the following description, at least part of the known structures and technologies are not shown to avoid unnecessary ambiguity in the present application; and, for clarity, the size of some structures may be exaggerated. In addition, the features, structures or characteristics described below may be combined in one or more embodiments in any suitable manner.

[0034] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish an entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations. In the description of the present application, it should be noted that, unless otherwise specified, the meaning of "multiple" is more than two. Moreover, the term "include", "comprise" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only include those elements, but also include other elements not clearly listed, or also include elements inherent to such process, method, article or equipment. In the absence of more restrictions, the elements limited by the sentence "include..." do not exclude the existence of other identical elements in the process, method, article or equipment including the elements.

[0035] Preparation method of fermented tea seed oil

[0036] The embodiment of the first aspect of the present application provides a method for increasing the ceramide content in fermented tea seed oil, comprising the following steps:

[0037] S10, taking a mixture containing tea seed oil and a culture medium and sterilizing it to obtain an initial fermentation system;

[0038] S20, inoculating Candida bombus into the initial fermentation system for fermentation culture until a first culture time is reached, and adding a carbon source;

[0039] S30, continuing the fermentation culture until the final culture time is reached to obtain a fermentation liquid;

[0040] S40, after the fermentation liquid is inactivated, stratified, centrifuged, dehydrated, and deodorized to obtain fermented tea seed oil; wherein,

[0041] The deposit number of Bombyx mossinus is CGMCC No.25797.

[0042] The preparation method of fermented tea seed oil provided in the embodiment of the present application adopts bumblebee Candida to ferment the tea seed oil. Bumblebee Candida can use the fatty acids sheared from the tea seed oil to further synthesize ceramide and bio-glycoside substances; at the same time, through the fed-batch fermentation process, the addition of a carbon source during the fermentation process can further increase the synthesis amount of ceramide, thereby increasing the content of ceramide in the tea seed oil, so that the obtained fermented tea seed oil has a higher ceramide content, which can be well applied to skin care products and has good antioxidant, skin moisturizing and skin barrier repair capabilities.

[0043] In some embodiments, the inoculation volume of Candida bombus is 5% to 10%, and optionally 6% to 8% of the total volume of the culture medium.

[0044] By limiting the inoculation amount of Bombyx mori in the fermentation broth within the above range, the effect of synthesizing ceramide can be further improved and the content of ceramide in the fermented tea seed oil can be increased.

[0045] In some embodiments, the culture medium includes yeast extract, peptone, soy protein, monopotassium phosphate, dipotassium phosphate, ammonium sulfate, and glycerol.

[0046] In some embodiments, based on the total mass of the culture medium, the mass content of potassium dihydrogen phosphate can be 3% to 7%, and the added amount of dipotassium hydrogen phosphate can be 10% to 20%; alternatively, based on the total mass of the culture medium, the mass content of potassium dihydrogen phosphate can be 4% to 6%, and the added amount of dipotassium hydrogen phosphate can be 12% to 17%.

[0047] Adding a certain proportion of phosphates such as potassium dihydrogen phosphate and dipotassium hydrogen phosphate to the culture medium can maintain the optimal buffer system required for ceramide synthesis. At the same time, a high proportion of phosphate helps to separate oil and water during the post-processing of fermented oil, including increasing the solubility of ceramide in the oil phase, reducing emulsification, and ultimately improving the extraction yield of fermented tea seed oil. The study found that when the content of potassium dihydrogen phosphate and dipotassium hydrogen phosphate in the culture medium is within the above range, the fermented tea seed oil has a higher ceramide content.

[0048] In some embodiments, based on the total volume of the culture medium, the added amount of tea seed oil can be 300 g / L to 600 g / L, optionally 350 g / L to 550 g / L.

[0049] By controlling the amount of tea seed oil added to the culture medium within the above range, the microorganisms in the culture medium can ferment the tea seed oil as fully as possible, thereby improving the fermentation effect. When the amount of tea seed oil added is small, the ceramide content in the obtained fermentation product is low; when the amount of tea seed oil added is large, it is difficult to completely ferment in the culture medium, and the purity of the fermentation product is low.

[0050] In some embodiments, the carbon source includes one or more of molasses, glucose, sucrose, soluble starch.

[0051] In some embodiments, the amount of carbon source added may be 10 g / L to 15 g / L, optionally 12 g / L to 14 g / L, based on the total volume of the culture medium.

[0052] In some embodiments, step S30 may include:

[0053] S31, continuing to culture the fermentation system with the added carbon source until the second culture time is reached, and adding serine;

[0054] S32, continuing the fermentation culture until the final culture time is reached to obtain a fermentation liquid;

[0055] In some embodiments, the amount of serine added is 1 g / L to 5 g / L, optionally 2 g / L to 4 g / L, based on the total volume of the culture medium.

[0056] Serine palmitoyltransferase (SPT) is a key rate-limiting enzyme in the biosynthesis pathway of ceramide in yeast. It can condense serine and palmitoyl-CoA into 3-ketodihydrosphingosine, which is the first step in the de novo synthesis pathway of ceramide. The addition of substrate serine in the fermentation system of the present application can further increase the amount of ceramide synthesized during the fermentation process, thereby increasing the content of ceramide in fermented tea seed oil, making it have better skin care effects.

[0057] In some embodiments, the first culture time can be 24h to 36h. Exemplarily, the first culture time can be cultured to 24h, 25h, 26h, 27h, 28h, 29h, 30h, 31h, 32h, 33h, 34h, 35h, 36h, or a range consisting of any of the above values.

[0058] In some implementations, the second culture time can be 42 h to 60 h. Exemplarily, the third culture time can be cultured to 42 h, 43 h, 44 h, 45 h, 46 h, 47 h, 48 h, 49 h, 50 h, 51 h, 52 h, 53 h, 54 h, 55 h, 56 h, 57 h, 58 h, 59 h, 60 h, or a range consisting of any of the above values.

[0059] In some embodiments, the final culture time can be 96h to 120h. Exemplarily, the second culture time can be 96h, 97h, 98h, 99h, 100h, 101h, 102h, 103h, 104h, 105h, 06h, 107h, 108h, 109h, 110h, 111h, 112h, 113h, 114h, 115h, 116h, 117h, 118h, 119h, 120h, or a range consisting of any of the above values.

[0060] In the present application, the first culture time, the second culture time and the final culture time all refer to the time from the start of culture to the end. For example, the first culture time of 24h to 36h refers to the 24th to 36th hour from the start of fermentation culture. Similarly, the second culture time refers to the 42nd to 610th hour from the start of fermentation culture. The final culture time refers to the 96th to 120th hour from the start of fermentation culture.

[0061] In some embodiments, the sterilization treatment conditions may be: moist heat sterilization at 117° C. to 123° C. for 20 min to 30 min.

[0062] In some embodiments, the temperature of the fermentation culture process may be 28°C to 32°C.

[0063] In some embodiments, the aeration rate during the fermentation culture process may be 0.8 vvm to 1.2 vvm.

[0064] In some embodiments, the stirring speed of the fermentation culture process can be 200 rpm to 800 rpm.

[0065] In some embodiments, during the fermentation process, the pH of the fermentation broth may be between 3.0 and 7.0.

[0066] In some embodiments, during the fermentation process, the dissolved oxygen content of the fermentation broth may be greater than or equal to 10%.

[0067] In some implementations, step S40 may include:

[0068] After the fermentation liquid is inactivated, it is allowed to stand and separate into layers, and the upper oil phase is collected by centrifugation;

[0069] The oil phase is dehydrated and deodorized by passing through an adsorption column; wherein,

[0070] The filler of the adsorption column can include diatomaceous earth, activated carbon and anhydrous sodium sulfate in a volume ratio of (2-4): (4-6): (1-3) from bottom to top. The total volume of the column bed of the adsorption column can be 20% of the total volume of the fermented tea seed oil, and the height-to-diameter ratio of the column bed can be 10:1.

[0071] Through the post-processing process of the triple physical adsorption column, fermented tea seed oil with little odor and low water content can be obtained quickly and conveniently, effectively reducing production costs. It is an energy-saving and green production process.

[0072] Fermented tea seed oil

[0073] The embodiment of the second aspect of the present application provides a fermented tea seed oil, which can be prepared according to the method of the embodiment of the first aspect of the present application.

[0074] In some embodiments, the content of ceramide in the fermented tea seed oil may be greater than or equal to 0.8%.

[0075] In some embodiments, the fermented tea seed oil contains a biosurfactant, which may be mannitol erythritol ester.

[0076] In some embodiments, the mass content of the biosurfactant in the fermented tea seed oil may be 0.1% to 0.5%.

[0077] application

[0078] The embodiment of the third aspect of the present application provides the use of the fermented tea seed oil obtained according to the preparation method of the embodiment of the first aspect of the present application or the fermented tea seed oil of the embodiment of the second aspect of the present application in skin care products.

[0079] Skin Care

[0080] The embodiment of the fourth aspect of the present application provides a skin care product, including fermented tea seed oil obtained according to the preparation method of the embodiment of the first aspect of the present application or the fermented tea seed oil of the embodiment of the second aspect of the present application.

[0081] In some embodiments, the fermented tea seed oil may be added in an amount of 0.1% to 80% in the skin care product.

[0082] In some embodiments, the skin care products may also include auxiliary materials commonly used in the cosmetics field. For example, the auxiliary materials may include but are not limited to one or more of preservatives, moisturizers, thickeners, emulsifiers, and neutralizers.

[0083] Example

[0084] The present invention is described below with reference to specific examples. It should be noted that the examples described below are exemplary and are only used to explain the present application, and should not be construed as limiting the present application. If specific techniques or conditions are not specified in the examples, they are carried out according to the techniques or conditions described in the literature in this area or according to the product specifications. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be obtained commercially.

[0085] The following are the sources of the fermentation bacteria used in the examples:

[0086] Starmerella bombicola: The preservation unit is China General Microbiological Culture Collection Center (No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing), the preservation number is CGMCC No.25797, and the preservation time is September 26, 2022.

[0087] Example 1

[0088] A fermented tea seed oil is prepared by the following method:

[0089] S10, mixing tea seed oil and culture medium and adding them into a fermentation tank, sterilizing at 120±3°C and then cooling to obtain an initial fermentation system; wherein, based on the volume of the culture medium used, the amount of tea seed oil added is 500 g / L, and the components of the culture medium include: yeast extract powder 6 g / L, peptone 3 g / L, soy protein 6 g / L, potassium dihydrogen phosphate 5 g / L, potassium dihydrogen phosphate 15 g / L, ammonium sulfate 3 g / L, glycerol 10 g / L and water 52 g / L to obtain an initial fermentation system;

[0090] S20, inoculating Starmerella bombicola Lip-DYH into the initial fermentation system and then fermenting and culturing, based on the volume of the culture medium used, the inoculation amount of Starmerella bombicola is 8% of the volume of the culture medium, and when the culture reaches 30 hours, adding carbon source glucose, based on the volume of the culture medium used, the amount of glucose added is 10 g / L, and continuing the fermentation and culturing to 108 hours to obtain a fermentation liquid;

[0091] S30, inactivating the fermentation liquid, standing and stratifying, taking out the upper crude oil phase, centrifuging the crude oil phase at 6000rpm for 10min, collecting the upper oil phase, passing the oil phase through a triple adsorption column, collecting the outflowing oil phase, and obtaining fermented tea seed oil; wherein the fillers of the adsorption column are diatomaceous earth, activated carbon and anhydrous sodium sulfate from bottom to top, and the ratio of the three is 2:5:1; the total volume of the column bed is 20% of the volume of the crude oil phase, and the height-to-diameter ratio of the column bed is 10:1.

[0092] Example 2

[0093] The difference from Example 1 is that S20 adds a step of adding substrate serine to the fermentation system. Specifically, S20 is as follows:

[0094] Inoculating Starmerella bombicola Lip-DYH into the initial fermentation system, and then fermenting and culturing, based on the volume of the culture medium used, the inoculation amount of Starmerella bombicola is 8% of the volume of the culture medium, adding carbon source glucose at the 30th hour, based on the volume of the culture medium used, the amount of glucose added is 12 g / L, and the fermentation and culturing is continued to the 48th hour, and then adding a sterilized serine aqueous solution, based on the volume of the culture medium used, the amount of serine added is 1 g / L (the weight volume ratio of serine to water in the added serine aqueous solution is 10%); continuing the fermentation and culturing to 108 hours to obtain a fermentation liquid;

[0095] The rest are consistent with Example 1.

[0096] Example 3

[0097] The difference from Example 2 is that the added amount of serine is 3 g / L, and the rest is consistent with Example 2.

[0098] Example 4

[0099] The difference from Example 2 is that the added amount of serine is 5 g / L, and the rest is consistent with Example 2.

[0100] The fermented tea seed oil and the unfermented tea seed oil obtained in Examples 1 to 4 were subjected to high performance liquid chromatography (HPLC) to detect the contents of ceramide AP and ceramide NP therein. Figure 1 HPLC spectra of ceramide AP and ceramide NP are shown.

[0101] The test conditions are as follows:

[0102] Chromatographic conditions: Waters 2424-ELSD; reversed phase liquid chromatography column: C18 (250×4.6 mm, 5 μm); ELSD parameters: drift tube temperature 40° C., nitrogen pressure 40 Psi, column temperature 35° C. Gradient elution conditions are shown in Table 1, and the detection column temperature is 40° C.

[0103] Table 1

[0104] Time / min Flow rate (mL / min) Methanol / %(v / v) Water / (v / v) 0 1.0 60 40 5 1.0 90 10 25 1.0 95 5 30 1.0 95 5 40 1.0 60 40 45 1.0 60 40

[0105] Reference substance:

[0106] Ceramide AP reference solution (100 μg / mL): weigh 0.0253 g of ceramide AP reference substance (99%) into a mL volumetric flask, add chromatographic methanol to dissolve and make up to volume, filter through a 0.22 μm microporous membrane, and inject;

[0107] Ceramide NP reference solution (100 μg / mL): weigh 0.0278 g of ceramide NP reference substance (90%) into a 250 mL volumetric flask, add chromatographic methanol to dissolve and make up to volume, filter through a 0.22 μm microporous membrane, and inject.

[0108] Ceramide AP reference substance and ceramide NP reference substance were both sourced from Chongqing Zhihe Biotechnology Co., Ltd.

[0109] Sample processing:

[0110] Take 5 mL of fermented tea seed oil, add 10 mL of methanol, shake vigorously and extract for 5 minutes, centrifuge at 10000 rpm, remove the upper methanol phase, add 10 mL of methanol to the oil phase, extract and centrifuge, collect all the methanol phases, pass through a 0.22 μm microporous filter membrane, and inject the sample; the data results are multiplied by the dilution factor of 4.

[0111] Comparison of ceramide content in the fermented tea seed oil prepared in Examples 1-4 Figure 2 shown.

[0112] according to Figure 2It can be seen that the content of ceramide AP in the fermented tea seed oil obtained by fermentation in Example 1 is nearly 3.5 times higher than that in the unfermented tea seed oil; different amounts of serine were added in Examples 2 to 4, and the contents of ceramide AP and ceramide NP in the fermented tea seed oil obtained were greatly improved. Among them, the content of ceramide AP in the fermented tea seed oil of Example 3 is nearly 13 times that of ceramide AP in the unfermented tea seed oil, and the contents of ceramide AP and ceramide NP in the fermented tea seed oil of Example 3 reached 3185.2 μg / mL and 230.6 μg / mL, respectively. This shows that the preparation method provided in the examples of the present application can significantly increase the content of ceramide in tea seed oil, and the addition of serine as a fermentation substrate has a more significant effect on increasing the content of ceramide.

[0113] The parameters of tea seed oil fermentation culture are further described below.

[0114] Embodiment 5-7

[0115] The difference from Example 1 is that the added amounts of potassium dihydrogen phosphate and dipotassium hydrogen phosphate in the culture medium are different, see Table 2 for details, and the rest are consistent with Example 1.

[0116] Examples 8 to 9

[0117] The difference from Example 1 is that the inoculation amount of Candida bombus in the culture medium is different, refer to Table 2 for details, and the rest is consistent with Example 1.

[0118] Example 10

[0119] The difference from Example 1 is that the added amount and type of carbon source are different, please refer to Table 2 for details, and the rest are consistent with Example 1.

[0120] Examples 11 to 12

[0121] The difference from Example 1 is that the inoculation amount of tea seed oil is different, please refer to Table 2 for details, and the rest is consistent with Example 1.

[0122] The fermented tea seed oil obtained in Examples 1, 5 to 12 was tested as follows:

[0123] (1) Conductivity detection of fermentation broth water phase:

[0124] Mettler conductivity meter-FiveEasy Plus was used for detection, and the specific detection method was carried out according to the instrument manual.

[0125] (2) Ceramide content detection:

[0126] High performance liquid chromatography (HPLC) was used for detection, and the detection method was the same as that of Examples 1 to 4.

[0127] (3) Detection of natural stratification oil phase yield:

[0128] Place the oil-water mixed fermentation liquid in a measuring cylinder, let it stand and separate into layers, then measure the volume of the oil layer V1. The volume of oil added during fermentation is taken as the initial test oil volume V0. The oil phase yield (%) is calculated using the formula V1 / V0×100%.

[0129] The stratification phenomenon of some fermentation broths in Examples 1, 5 and 6 was compared, and the comparison results were referred to Figure 3 .

[0130]

[0131] Combining the data in Table 2 and Figure 3 It can be seen that the higher the content of potassium dihydrogen phosphate and dipotassium hydrogen phosphate in the culture medium, the more obvious the oil phase static stratification, and the higher the yield of the oil phase after static natural stratification. The addition amount of potassium dihydrogen phosphate and dipotassium hydrogen phosphate also affects the content of ceramide. Adding a certain proportion of phosphates such as potassium dihydrogen phosphate and dipotassium hydrogen phosphate to the culture medium can make the final fermented tea seed oil have a higher ceramide content.

[0132] In summary, according to the technical solution provided by the present application, a fermented tea seed oil with significantly increased ceramide content can be prepared. A large number of existing research results show that ceramide plays a very important role in maintaining skin barrier and skin stabilization function, and ceramide has a strong ability to associate water molecules, which maintains skin moisture by forming a mesh structure in the stratum corneum, thereby having the effect of maintaining skin moisture. In addition, ceramide also has the effects of soothing and anti-inflammatory, increasing the thickness of the epidermal stratum corneum, reducing wrinkles, enhancing skin elasticity, and delaying skin aging. Therefore, the fermented tea seed oil prepared by the technical solution provided by the present application has better barrier repair and skin stabilization function for human skin cells, and is also more significant in terms of soothing and anti-inflammatory, moisturizing and anti-oxidation, and can be better applied to skin care products, broadening the application value of fermented tea seed oil in skin care products.

[0133] Although the present application has been described with reference to preferred embodiments, various modifications may be made thereto and parts thereof may be replaced with equivalents without departing from the scope of the present application. In particular, the various technical features mentioned in the various embodiments may be combined in any manner as long as there are no structural conflicts. The present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A method for increasing the content of ceramide in fermented tea seed oil, characterized in that: The steps include: Taking a mixture containing tea seed oil and culture medium and sterilizing it to obtain an initial fermentation system; Inoculating Candida bombus into the initial fermentation system for fermentation and culturing until a first culturing time is reached, and adding a carbon source; Continuing the fermentation culture until the final culture time is reached to obtain a fermentation liquid; After the fermentation liquid is inactivated, it is subjected to layering, centrifugation, dehydration and deodorization treatment to obtain fermented tea seed oil; wherein, The deposit number of the bumblebee Candida is CGMCC No.25797.

2. The method according to claim 1, characterized in that Based on the volume of the culture medium, the inoculation volume of the Candida bombus is 5% to 10% of the volume of the culture medium.

3. The method according to claim 1, characterized in that The culture medium comprises yeast extract powder, peptone, soybean protein, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium sulfate, glycerol and water; Optionally, based on the total mass of the culture medium, the mass content of potassium dihydrogen phosphate is 3% to 7%, and the added amount of dipotassium hydrogen phosphate is 10% to 20%.

4. The method according to claim 1, characterized in that: Based on the volume of the culture medium, the added amount of the tea seed oil is 300-600 g / L.

5. The method according to claim 1, characterized in that The carbon source includes one or more of molasses, glucose, sucrose, and soluble starch; Optionally, based on the volume of the culture medium, the added amount of the carbon source is 10 g / L to 15 g / L.

6. The method according to claim 1, characterized in that The step of continuing the fermentation culture until the final culture time is reached to obtain the fermentation liquid comprises: The fermentation system with the added carbon source is continuously cultured until the second culture time is reached, and serine is added; Continuing the fermentation culture until the final culture time is reached to obtain a fermentation liquid; Optionally, based on the volume of the culture medium, the amount of serine added is 1 g / L to 5 g / L.

7. The method according to claim 6, characterized in that The first culture time is 24h to 36h; and / or The second culture time is 42h to 60h; and / or The final culture time is 96h to 120h.

8. A fermented tea seed oil, characterized in that: It is prepared by the method according to any one of claims 1 to 7.

9. Use of the fermented tea seed oil prepared by the method according to any one of claims 1 to 7 or the fermented tea seed oil according to claim 8 in skin care products.

10. A skin care product, characterized in that: The fermented tea seed oil prepared by the method according to any one of claims 1 to 7 or the fermented tea seed oil according to claim 8; Optionally, the mass content of the fermented tea seed oil in the skin care product is 0.1% to 80%.

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