A method for cultivating puffballs using soil-rot pine mycorrhizal fungi as a substitute material and an inoculation auxiliary device

Abstract

The invention discloses a method for cultivating puffballs using soil-rot pine wood mycorrhizal fungi as a substitute for raw materials and an inoculation auxiliary device, which relate to the technical field of puffball inoculation. The invention comprises a fixed sleeve, wherein a T-shaped push rod is slidably connected to the interior of the fixed sleeve, and further comprises: an inoculation leak-proof mechanism and an auxiliary matching mechanism. Through the arrangement of the inoculation leak-proof mechanism, not only can real-time protection be provided when the fixed sleeve is not used to extract and inoculate the puffball fungus species, so that a relatively closed space is formed at the extraction end of the fixed sleeve, which effectively prevents microorganisms, dust and other pollutants in the external environment from entering the interior of the container, thereby maintaining the high purity and activity of the subsequent extracted fungus species, but also the extraction end of the fixed sleeve can be closed and covered after the fixed sleeve is used to extract the material, thereby effectively preventing the fungus species inside the fixed sleeve from falling off or leaking out during the process of the operator extracting and moving the material for inoculation, thereby ensuring the consistency of the number of fungus species inoculated each time.

Description

Technical Field

[0001] The invention relates to the technical field of puffball inoculation, in particular to a method for cultivating puffballs using soil-rot pine wood mycorrhizal fungi as a substitute material and an inoculation auxiliary device. Background Art

[0002] In the existing technology, the use of soil-rotten pine wood mycorrhizal fungi as a substitute for puffball inoculation refers to a technology that uses soil-rotten pine wood and the mycorrhizal fungi on it as a culture medium to replace traditional culture materials to cultivate puffballs. When used, it can improve the soil environment, increase soil fertility, and provide good nutritional conditions for the growth of puffballs, thereby promoting their growth and development.

[0003] In the process of puffball cultivation, it is usually necessary to use a related inoculator, which is inserted into a container containing puffball spawn to extract the puffball spawn, and then the inoculator after extracting the spawn is moved to the mushroom bag position, and the puffball spawn material taken from the inoculator is transported to the mushroom bag to complete the inoculation of the puffball spawn. However, the existing inoculator structure is usually composed of a sleeve and a push rod, and the front end of the sleeve is used to extract the puffball spawn. Due to the single structure of the sleeve and the long exposure to the external environment, when the material is not extracted and inoculated, the sleeve in an exposed state is The material-taking end cannot prevent pollutants such as microorganisms and dust from the external environment from entering the inoculator, thereby contaminating the strains, reducing their purity and activity, and affecting the subsequent cultivation effect. At the same time, when the operator uses the sleeve to take materials and move the inoculation, due to the lack of a stable closed covering mechanism, the strains inside the sleeve are prone to falling or leaking, which not only leads to inconsistent numbers of strains inoculated each time, but also increases the risk of strain waste and contamination, thereby affecting the cultivation effect of puffball strains and reducing the success rate and yield of cultivation.

[0004] In view of this, the present invention proposes a method for cultivating puffballs using soil-rot pine mycorrhizal fungi as a substitute material and an inoculation auxiliary device to remedy and improve the shortcomings of the existing technology. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides a method for cultivating puffballs using soil-rot pine mycorrhizal fungi as a substitute material and an inoculation auxiliary device to solve the corresponding technical problems raised in the above background technology.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a method for inoculating puffballs with soil-rot pine mycorrhizal fungi as a substitute for material cultivation, the inoculation method comprising the following steps:

[0007] Step 1: Prepare the inoculation environment and materials required for inoculation;

[0008] Step 2: Use an inoculator to inoculate the puffball strain;

[0009] Step 3: Seal the inoculated bags and carry out subsequent management.

[0010] The step 1 includes: first, ensuring that the inoculation room has been strictly disinfected, preparing the inoculation gun, bacteria, disinfected bacteria bags and substitute materials, and the inoculation gun needs to be debugged in advance to ensure a large spraying area and sufficient pressure.

[0011] The second step includes: disinfecting the inoculation gun tip with 75% alcohol, inserting it into the bottom of the fungus bag, starting the inoculation gun, and evenly spraying the fungus from bottom to top until the fungus fills the bottom of the fungus bag, taking care to avoid spraying the fungus outside the bag to ensure an appropriate inoculation amount.

[0012] The step three includes: after the inoculation is completed, quickly seal the bag with a sponge block or a plastic cover to ensure that the tightness is appropriate and there is no leakage, move the bag to the culture room, control the temperature at 16-26°C, maintain a low light or dark environment, observe the growth of the bag every day, and adjust ventilation and humidity to keep the temperature constant.

[0013] The invention relates to an auxiliary device for inoculating puffballs for cultivation of soil-rot pine mycorrhizal fungi as a substitute material, comprising a fixed sleeve, a T-shaped push rod slidably connected to the interior of the fixed sleeve, and an inoculation leak-proof mechanism and an auxiliary matching mechanism, wherein the inoculation leak-proof mechanism and the auxiliary matching mechanism are both arranged at one end of the fixed sleeve away from the T-shaped push rod;

[0014] The inoculation leak-proof mechanism is used to protect the fixed sleeve when it is not inoculated, and to close the end of the fixed sleeve away from the T-shaped push rod after the fixed sleeve is inoculated;

[0015] The auxiliary cooperation mechanism is used in conjunction with the inoculation leakage prevention mechanism.

[0016] Preferably, the inoculation leak-proof mechanism includes a first operating frame and a second operating frame that are slidably connected to the outer surface of the fixed sleeve, and the first operating frame and the second operating frame are fixedly connected, the outer surface of the first operating frame is slidably connected to a rectangular movable frame, and the outer surfaces on both sides of the first operating frame are symmetrically provided with sliding grooves, and a sliding column is slidably connected in the sliding groove, and the end of the sliding column away from the first operating frame is fixedly connected to the rectangular movable frame.

[0017] Preferably, the vaccination leakage prevention mechanism also includes a half-frame protective cover symmetrically arranged on the end of the fixed sleeve away from the T-shaped push rod, the half-frame protective cover is symmetrically fixedly connected to a driven plate on the opposite side, the driven plate is fixedly connected to the first operating frame at one end facing the rectangular movable frame with a first elastic telescopic column, the half-frame protective cover is symmetrically fixedly connected to fixed columns on both sides, and a linkage plate is rotatably connected between the fixed column and the sliding column.

[0018] Preferably, an ear plate is symmetrically fixedly connected to the outer surface of one end of the second operating frame away from the first operating frame, a push plate is fixedly connected to the side of the fixed sleeve away from the first operating frame, and a second elastic telescopic column is symmetrically fixedly connected between the push plate and the ear plate.

[0019] Preferably, the auxiliary cooperation mechanism includes a fixed plate fixedly connected to the outer surface of the rectangular movable frame, the outer surface of the end of the first operating frame away from the rectangular movable frame is fixedly connected to a support column, the end of the support column away from the first operating frame is fixedly connected to a U-shaped positioning frame, the end of the fixed plate facing the U-shaped positioning frame is fixedly connected to a connecting column, and the end of the connecting column away from the fixed plate is fixedly connected to a limiting chuck.

[0020] Preferably, the U-shaped positioning frame is symmetrically connected to sliding rods on both sides, and the opposite ends of the sliding rods are fixedly connected to inclined blocks. The opposite sides of the inclined blocks facing the limit chuck are set as inclined surfaces, and the opposite sides of the inclined blocks away from the limit chuck are provided with limiting slots. A reset spring is fixedly connected between the inclined block and the U-shaped positioning frame, and the reset spring is sleeved on the outer surface of the sliding rod.

[0021] Preferably, the sliding rods are fixedly connected to movable plates at one end away from each other, one of the movable plates is fixedly connected to a pull rod at the side away from the U-shaped positioning frame, support plates are symmetrically fixedly connected on both sides of the U-shaped positioning frame, the support plate is rotatably connected to a center axis at the side away from the first operating frame, the outer surface of the end of the center axis away from the support plate is fixedly connected to a center plate, and follower plates are symmetrically rotatably connected between the two ends of the center plate and the movable plate.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) Through the setting of the inoculation leak-proof mechanism, the two half-frame protective covers that are initially locked together can not only provide real-time protection when the fixed sleeve is not used to take the puffball strains for inoculation, but also form a relatively closed space at the taking end of the fixed sleeve, effectively preventing microorganisms, dust and other pollutants in the external environment from entering the container, thereby maintaining the high purity and activity of the subsequent taken strains, and the setting of the half-frame protective cover can also close and cover the taking end of the fixed sleeve after the fixed sleeve is used to take the materials, thereby effectively preventing the strains inside the fixed sleeve from falling or leaking during the process of the operator taking the materials and moving them for inoculation, ensuring the consistency of the number of strains inoculated each time, making the puffball cultivation process more stable and reliable, thereby improving the success rate and yield of the cultivation.

[0024] (2) By setting up an auxiliary matching mechanism, utilizing the design of the inclined surface on the inclined block and the limit card slot, and cooperating with the synchronous follow-up movement of the limit chuck, when the limit chuck moves, the inclined surface can provide a guiding function, so that the limit chuck can be stably moved to the limit chuck for limitation, thereby achieving effective limitation of the position of the rectangular moving frame after movement, avoiding the rectangular moving frame from loosening during the subsequent operator's collection of puffball fungus, causing the half-frame protective cover to reset and affecting the collection and inoculation of the puffball fungus. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of a preferred embodiment of the present invention;

[0026] Figure 2 This is a schematic diagram of the disassembled structure of the fixed sleeve and the T-shaped push rod shown in the present invention;

[0027] Figure 3 This is a schematic diagram of the connection structure of the rectangular movable frame shown in the present invention;

[0028] Figure 4 This is a schematic diagram of the structure of the connection of the half-frame protective cover shown in the present invention;

[0029] Figure 5 This is a structural diagram of the connection between the first operating frame and the second operating frame shown in the present invention;

[0030] Figure 6 This is a schematic diagram of the structure of the U-shaped positioning frame connection shown in the present invention;

[0031] Figure 7 The present invention shows Figure 6 Schematic diagram of the method structure at A.

[0032] The numbers in the figure are:

[0033] 1. Fixed sleeve; 2. T-shaped push rod;

[0034] 3. Inoculation leak prevention mechanism; 301. First operating frame; 302. Second operating frame; 303. Slide; 304. Rectangular movable frame; 305. Sliding column; 306. Linkage plate; 307. Fixed column; 308. Half-frame protective cover; 309. Follower plate; 310. First elastic telescopic column; 311. Ear plate; 312. Second elastic telescopic column; 313. Push plate;

[0035] 4. Auxiliary matching mechanism; 401. Fixed plate; 402. Connecting column; 403. Limiting chuck; 404. Support column; 405. U-shaped positioning frame; 406. Sliding rod; 407. Return spring; 408. Bevel block; 409. Limiting slot; 410. Pull rod; 411. Moving plate; 412. Support plate; 413. Center axis; 414. Center plate; 415. Follower plate. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] Embodiment 1 of the present invention:

[0038] A method for inoculating puffballs with soil-rot pine mycorrhizal fungi as a substitute for material cultivation, the inoculation method comprising the following steps:

[0039] Step 1: Prepare the inoculation environment and materials required for inoculation;

[0040] Step 2: Use an inoculator to inoculate the puffball strain;

[0041] Step 3: Seal the inoculated bags and perform subsequent management.

[0042] Step 1 includes: First, ensure that the inoculation room has been strictly disinfected, prepare the inoculation gun, fungus, disinfected fungus bags and substitute materials (such as sawdust, wheat bran, etc.), and the inoculation gun needs to be debugged in advance to ensure a large spray area and sufficient pressure.

[0043] Step 2 includes: disinfecting the inoculation gun tip with 75% alcohol, inserting it into the bottom of the fungus bag, starting the inoculation gun, and spraying the fungus evenly from bottom to top until the fungus fills the bottom of the fungus bag. Be careful to avoid spraying the fungus outside the bag and ensure the inoculation amount is appropriate.

[0044] Step three includes: After inoculation is completed, quickly seal the bag with a sponge or plastic cover to ensure that it is appropriately tight and there is no leakage. Move the bag to the culture room, control the temperature between 16 and 26 degrees Celsius, maintain a low light or dark environment, observe the growth of the bag every day, and adjust ventilation and humidity to keep the temperature constant.

[0045] Embodiment 2 of the present invention:

[0046] Please refer to Figures 1 to 7As shown, the inoculation auxiliary device for the cultivation of puffballs with soil-rot pine mycorrhizal fungi as a substitute material comprises a fixed sleeve 1, a T-shaped push rod 2 is slidably connected to the interior of the fixed sleeve 1, and further comprises: an inoculation leak-proof mechanism 3 and an auxiliary matching mechanism 4, and the inoculation leak-proof mechanism 3 and the auxiliary matching mechanism 4 are both arranged at the end of the fixed sleeve 1 away from the T-shaped push rod 2;

[0047] The inoculation leak-proof mechanism 3 is used to protect the fixed sleeve 1 when it is not inoculated, and to close the end of the fixed sleeve 1 away from the T-shaped push rod 2 after the fixed sleeve 1 is inoculated;

[0048] The auxiliary cooperation mechanism 4 is used in conjunction with the inoculation leakage prevention mechanism 3;

[0049] The inoculation leak prevention mechanism 3 includes a first operating frame 301 and a second operating frame 302 that are slidably connected to the outer surface of the fixed sleeve 1, and the first operating frame 301 and the second operating frame 302 are fixedly connected. The outer surface of the first operating frame 301 is slidably connected to a rectangular movable frame 304. The outer surfaces of both sides of the first operating frame 301 are symmetrically provided with sliding grooves 303. A sliding column 305 is slidably connected in the sliding grooves 303, and the end of the sliding column 305 away from the first operating frame 301 is fixedly connected to the rectangular movable frame 304.

[0050] The inoculation leak-proof mechanism 3 also includes a half-frame protective cover 308 symmetrically arranged on the end of the fixed sleeve 1 away from the T-shaped push rod 2, and a driven plate 309 is symmetrically fixedly connected to the side away from the half-frame protective cover 308. A first elastic telescopic column 310 is fixedly connected between the end of the driven plate 309 facing the rectangular movable frame 304 and the first operating frame 301. Fixed columns 307 are symmetrically fixedly connected on both sides of the half-frame protective cover 308, and a linkage plate 306 is rotatably connected between the fixed column 307 and the sliding column 305;

[0051] An ear plate 311 is symmetrically fixedly connected to the outer surface of one end of the second operating frame 302 away from the first operating frame 301 , a push plate 313 is fixedly connected to the side of the fixed sleeve 1 away from the first operating frame 301 , and a second elastic telescopic column 312 is symmetrically fixedly connected between the push plate 313 and the ear plate 311 .

[0052] The effects achieved by this embodiment are as follows: compared with the prior art, through the setting of the inoculation leak-proof mechanism 3, the two half-frame protective covers 308 that are initially locked together can not only provide real-time protection when the fixed sleeve 1 is not used to take materials for inoculation of puffball strains, but also form a relatively closed space at the taking end of the fixed sleeve 1, effectively preventing microorganisms, dust and other pollutants in the external environment from entering the interior of the container, thereby maintaining the high purity and activity of subsequent taken strains, and the setting of the half-frame protective cover 308 can also close and cover the taking end of the fixed sleeve 1 after the fixed sleeve 1 is used to take materials, thereby effectively avoiding the falling and leakage of strains inside the fixed sleeve 1 during the process of the operator taking materials and moving for inoculation, ensuring the consistency of the number of strains inoculated each time, making the puffball cultivation process more stable and reliable, thereby improving the success rate and yield of cultivation.

[0053] Further examples:

[0054] Please refer to Figures 1 to 7 As shown, the auxiliary cooperation mechanism 4 includes a fixed plate 401 fixedly connected to the outer surface of the rectangular movable frame 304, a support column 404 is fixedly connected to the outer surface of one end of the first operating frame 301 away from the rectangular movable frame 304, and a U-shaped positioning frame 405 is fixedly connected to the end of the support column 404 away from the first operating frame 301. A connecting column 402 is fixedly connected to the end of the fixing plate 401 facing the U-shaped positioning frame 405, and a limiting chuck 403 is fixedly connected to the end of the connecting column 402 away from the fixing plate 401.

[0055] Sliding rods 406 are symmetrically connected to the U-shaped positioning frame 405 on both sides, and the opposite ends of the sliding rods 406 are fixedly connected to inclined blocks 408. The sides of the inclined blocks 408 facing the limit chuck 403 are set as inclined surfaces, and the sides of the inclined blocks 408 facing the limit chuck 403 are provided with limit slots 409. A return spring 407 is fixedly connected between the inclined block 408 and the U-shaped positioning frame 405, and the return spring 407 is sleeved on the outer surface of the sliding rod 406.

[0056] The sliding rod 406 is fixedly connected to a movable plate 411 at one end away from the sliding rod 406, and a pull rod 410 is fixedly connected to one side of the movable plate 411 away from the U-shaped positioning frame 405, and support plates 412 are symmetrically fixedly connected on both sides of the U-shaped positioning frame 405. The support plate 412 is rotatably connected to the side away from the first operating frame 301, and the outer surface of the end of the central axis 413 away from the support plate 412 is fixedly connected to the center plate 414, and follower plates 415 are symmetrically rotatably connected between the two ends of the center plate 414 and the movable plate 411.

[0057] The effects achieved by this embodiment are as follows: compared with the prior art, through the setting of the auxiliary matching mechanism 4, utilizing the design of the inclined surface on the inclined block 408 and the limiting slot 409, cooperating with the synchronous following movement of the limiting chuck 403, when the limiting chuck 403 moves, the inclined surface can provide a guiding function, so that the limiting chuck 403 can be stably moved to the limiting chuck 403 for limitation, thereby achieving effective limitation of the position of the rectangular moving frame 304 after movement, avoiding the rectangular moving frame 304 from loosening during the subsequent operator's collection of puffball strains, causing the half-frame protective cover 308 to reset and affecting the collection and inoculation of puffball strains.

[0058] The complete usage steps and working principle of the above embodiment are as follows:

[0059] In the initial state, the T-shaped push rod 2 is slidably set on the fixed sleeve 1, and when the puffball strains are not being taken, the T-shaped push rod 2 is at the rear end of the fixed sleeve 1, and the front end of the fixed sleeve 1 is used to take the puffball strains. When taking the materials, the operator can insert the fixed sleeve 1 into the container containing the strains to take the strains. After the taking action is completed, the fixed sleeve 1 can be inserted into the fungus bag, and the material taken at the front end of the fixed sleeve 1 can be pushed out by pushing the T-shaped push rod 2, so that the fungus material enters the fungus bag, thereby completing the taking and inoculation of the puffball strains.

[0060] Before inserting the fixed sleeve 1 into the container containing the bacterial strains to take out the bacterial strains, Figures 1 to 4 As shown, since the outer surface of the fixed sleeve 1 is slidably provided with a first operating frame 301, and the outer surface of the first operating frame 301 is slidably provided with a rectangular movable frame 304, and the outer surface of the first operating frame 301 is symmetrically provided with a slide groove 303, a slide post 305 is slidably provided in the slide groove 303, and the slide post 305 is fixedly connected to the rectangular movable frame 304. When in use, the operator can control the first operating frame 301 and, based on the first operating frame 301, push the rectangular movable frame 304 on the first operating frame 301 to make the rectangular movable frame 304 move. The shaped moving frame 304 moves toward the front end of the fixed sleeve 1 (i.e., the end away from the T-shaped push rod 2), and the sliding column 305 will move synchronously in the sliding groove 303. In addition, because the front end of the fixed sleeve 1 is symmetrically provided with two half-frame protective covers 308, the two half-frame protective covers 308 are initially connected to each other in one body, and the front end of the fixed sleeve 1 is covered to achieve protection of the front end of the fixed sleeve 1 (i.e., the material taking and inoculation end) when not in use, so as to avoid damage to the front end of the fixed sleeve 1 affecting the subsequent material taking and inoculation of the puffball strains. Figure 3 and Figure 4As shown, a driven plate 309 is fixedly provided on the side away from the two half-frame protective covers 308, and a first elastic telescopic column 310 is fixedly provided on the driven plate 309 toward one end of the rectangular movable frame 304 and the outer wall of the first operating frame 301, and a fixed column 307 is symmetrically fixed on the half-frame protective cover 308, and a linkage plate 306 is rotatably provided between the fixed column 307 and the sliding column 305. Therefore, when the operator pushes the rectangular movable frame 304 toward the half-frame protective cover 308, so that the sliding column 305 slides synchronously on the outer wall of the first operating frame 301, the linkage plate 306 rotates synchronously under the influence of the sliding column 305, and drives it away from the fixed column 307 set at one end of the sliding column 305, so that the two The half-frame protective cover 308 moves in opposite directions therebetween. It should be noted that the two linkage plates 306 initially form a V-shape. When the sliding column 305 pushes the linkage plate 306, the two linkage plates 306 can be driven to open outward, thereby opening the two half-frame protective covers 308, thereby releasing the clamping relationship between the two half-frame protective covers 308 and increasing the distance between the two half-frame protective covers 308 to facilitate the subsequent passage of the fixed sleeve 1. At the same time, since the half-frame protective cover 308 is connected to the first operating frame 301 through the driven plate 309 and the first elastic telescopic column 310, when the half-frame protective cover 308 is driven to move outward, the driven plate 309 moves outward synchronously and drives the first elastic telescopic column 310 to stretch, as shown in FIG. Figures 4 to 6As shown, since a fixed plate 401 is fixedly provided on the rectangular movable frame 304, and the fixed plate 401 is fixedly connected to the limiting chuck 403 on one side facing the half-frame protective cover 308 through a connecting column 402, and a support column 404 is fixedly provided on the outer surface of one end of the first operating frame 301 facing the half-frame protective cover 308 in the same direction as the fixed plate 401, and a U-shaped positioning frame 405 is fixedly provided on the support column 404. When the operator pushes the rectangular movable frame 304 toward the half-frame protective cover 308, the position of the fixed plate 401 is synchronously changed, and the limiting chuck 403 is synchronously driven to be pushed toward the direction of the U-shaped positioning frame 405. Since sliding rods 406 are symmetrically provided on both sides of the U-shaped positioning frame 405, inclined blocks 408 are fixedly provided at the opposite ends of the sliding rods 406, and initially, the two inclined blocks 408 are both set to inclined surfaces facing one end of the rectangular movable frame 304, and the inclined surfaces are connected. The distance between them gradually decreases from one end toward the rectangular moving frame 304 to the end away from the rectangular moving frame 304. As the rectangular moving frame 304 continues to move, the limiting chuck 403 will enter between the two inclined blocks 408, and the outer surface of the limiting chuck 403 will contact the facing surfaces of the two inclined blocks 408. After the limiting chuck 403 continues to advance, under the action of the inclined surface, the two inclined blocks 408 will move in opposite directions between them, and the sliding rod 406 will move synchronously on the two ends of the U-shaped positioning frame 405. Because a return spring 407 is fixedly provided between the U-shaped positioning frame 405 and the inclined block 408, and the return spring 407 is sleeved on the outer surface of the sliding rod 406, when the inclined block 408 is pushed outward by the limiting chuck 403 and drives the sliding rod 406 to move synchronously through the U-shaped positioning frame 405, the return spring 407 is compressed synchronously. Figure 6 As shown, and because the two oblique blocks 408 are all provided with limiting draw-in groove 409 on the opposite sides of one end away from rectangular movable frame 304, as limiting chuck 403 continues to move, after limiting chuck 403 passes the inclined plane completely, can enter between limiting draw-in groove 409, the outward thrust that limiting chuck 403 produces to oblique block 408 disappears at this moment, under the elastic force of return spring 407, the two oblique blocks 408 are moved to the middle and reset, and limiting chuck 403 also is synchronously stuck in limiting draw-in groove 409, utilize the setting of limiting draw-in groove 409, limiting chuck 403 is carried out position limitation, thereby realizes the position limitation after rectangular movable frame 304 is moved, avoids the situation that rectangular movable frame 304 is loosened in subsequent material fetching process, causes half frame guard cover 308 to reset and affects the material fetching and inoculation of puffball fungus species;

[0061] After the operator opens the two half-frame protective covers 308 by moving the rectangular movable frame 304 and limits the limit chuck 403 by using the design of the limit card slot 409, and simultaneously limits the position of the half-frame protective covers 308 after opening, the operator can continue to take the first operating frame 301, such as Figure 1 and Figure 2 When the second locking cam 312 is engaged, the locking cam 313 is engaged with the locking cam 314 and the locking cam 315. As shown in FIG, since the first operating frame 301 and the second operating frame 302 are fixedly connected, the ear plates 311 are symmetrically fixedly arranged on the second operating frame 302, the rear end of the fixed sleeve 1 (i.e., the end away from the half-frame protective cover 308) is fixedly connected to the push plate 313, and a second elastic telescopic column 312 is symmetrically fixedly arranged between the push plate 313 and the ear plate 311. The operator can continue to use the first operating frame 301 and the second operating frame 302 as a basis to push the push plate 313 toward the container containing the puffball spawn, so that the second elastic telescopic column 312 is compressed, and the front end of the fixed sleeve 1 can be inserted into the container to extract the puffball spawn inside the container. After the extraction is completed, the pushing control of the push plate 313 can be released, so that the push plate 313 moves in the direction away from the second operating frame 302 under the elastic force of the second elastic telescopic column 312, and the position of the fixed sleeve 1 is restored. The design of the second elastic telescopic column 312 makes it easy for the fixed sleeve 1 to be inserted into and out of the container. Figure 6 and Figure 7As shown, since one end of the sliding rod 406 away from the inclined block 408 is fixedly connected to the moving plate 411, and a pull rod 410 is fixedly provided on one of the moving plates 411, and support plates 412 are symmetrically fixedly provided on both sides of the U-shaped positioning frame 405, a central axis 413 is rotatably provided on the support plate 412, a central plate 414 is fixedly provided on the outer surface of the central axis 413, and a follower plate 415 is symmetrically rotatably provided between the central plate 414 and the moving plate 411, after the fixed sleeve 1 takes the material and resets, the operator can pull the pull rod 410 outward to make the moving plate 411 move synchronously, and under the connection action of the follower plate 415, the center plate 414 can be synchronously driven to rotate around the center axis 413 as the axis, thereby driving the other follower plate 415 to move, and the other moving plate 411 is synchronously pushed outward. At this time, the two sliding The rod 406 slides outward synchronously, which can pull the inclined block 408 to move synchronously, thereby increasing the distance between the two inclined blocks 408. Through the elastic force of the first elastic telescopic column 310 mentioned above, the two half-frame protective covers 308 can be pushed in the directions toward each other, and under the connection action of the linkage plate 306, the rectangular movable frame 304 is synchronously driven to return to the initial position. As the rectangular movable frame 304 moves, the limit chuck 403 is synchronously separated from the limit slot 409. At this time, the two half-frame protective covers 308 will re-form a closed clamping relationship, and the front end of the fixed sleeve 1 will be covered and wrapped, so as to achieve the blocking of the material taking port of the fixed sleeve 1, and avoid the situation where the bacteria taken by the fixed sleeve 1 fall or leak during the movement of the operator, so that the number of bacteria inoculated each time is different, which affects the cultivation of puffballs.

[0062] The circuits and controls involved in the present invention are all prior art and will not be described in detail here.

[0063] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for inoculating puffballs with soil-rot pine mycorrhizal fungi as a substitute for material cultivation, characterized in that: The steps of the inoculation method include: Step 1: Prepare the inoculation environment and materials required for inoculation; Step 2: Use an inoculator to inoculate the puffball strain; Step 3: Seal the inoculated bags and perform subsequent management; The step 1 includes: first, disinfecting the inoculation room, preparing the inoculation gun, bacterial strains, disinfected bacterial bags and substitute materials; The second step includes: disinfecting the inoculation gun tip with 75% alcohol, inserting it into the bottom of the fungus bag, starting the inoculation gun, and spraying the fungus evenly from bottom to top; The third step includes: after the inoculation is completed, the bag is sealed and moved to the culture room, the temperature of the culture room is adjusted to 16-26°C to maintain the growth environment of the bacteria, the growth of the bag is observed every day, and ventilation and humidity are adjusted to maintain a constant temperature; The inoculation gun comprises a fixed sleeve (1), a T-shaped push rod (2) is slidably connected to the interior of the fixed sleeve (1), and further comprises: an inoculation leak-proof mechanism (3) and an auxiliary matching mechanism (4), and the inoculation leak-proof mechanism (3) and the auxiliary matching mechanism (4) are both arranged at one end of the fixed sleeve (1) away from the T-shaped push rod (2); The inoculation leak-proof mechanism (3) is used to provide protection when the fixed sleeve (1) is not inoculated, and to close the end of the fixed sleeve (1) away from the T-shaped push rod (2) after the fixed sleeve (1) is inoculated; The auxiliary matching mechanism (4) is used in conjunction with the inoculation leak prevention mechanism (3).

2. A method for cultivating puffballs by using soil-rot pine mycorrhizal fungi as a substitute material according to claim 1, characterized in that: The inoculation leak-proof mechanism (3) includes a first operating frame (301) and a second operating frame (302) which are slidably connected to the outer surface of the fixed sleeve (1), and the first operating frame (301) and the second operating frame (302) are fixedly connected. The outer surface of the first operating frame (301) is slidably connected to a rectangular movable frame (304). The outer surfaces of both sides of the first operating frame (301) are symmetrically provided with sliding grooves (303). A sliding column (305) is slidably connected in the sliding groove (303), and the sliding column (305) is fixedly connected to the rectangular movable frame (304) at one end away from the first operating frame (301).

3. a kind of soil decay pine mycorrhizal fungus substitute material cultivation puffball inoculation method according to claim 2, is characterized in that, The inoculation leak prevention mechanism (3) also includes a semi-frame protective cover (308) symmetrically arranged on one end of the fixed sleeve (1) away from the T-shaped push rod (2), and the semi-frame protective cover (308) is symmetrically fixedly connected to a driven plate (309) on the side away from it, and the driven plate (309) is fixedly connected to the first operating frame (301) with a first elastic telescopic column (310) between one end facing the rectangular movable frame (304), and the semi-frame protective cover (308) is symmetrically fixedly connected to fixed columns (307) on both sides, and a linkage plate (306) is rotatably connected between the fixed column (307) and the sliding column (305).

4. A method for cultivating puffballs by using soil-rot pine mycorrhizal fungi as a substitute for raw materials according to claim 2, characterized in that: An ear plate (311) is symmetrically fixedly connected to the outer surface of one end of the second operating frame (302) away from the first operating frame (301), a push plate (313) is fixedly connected to the side of the fixed sleeve (1) away from the first operating frame (301), and a second elastic telescopic column (312) is symmetrically fixedly connected between the push plate (313) and the ear plate (311).

5. A method for cultivating puffballs by using soil-rot pine mycorrhizal fungi as a substitute for raw materials according to claim 2, characterized in that: The auxiliary matching mechanism (4) comprises a fixing plate (401) fixedly connected to the outer surface of the rectangular movable frame (304); a support column (404) is fixedly connected to the outer surface of one end of the first operating frame (301) away from the rectangular movable frame (304); an end of the support column (404) away from the first operating frame (301) is fixedly connected to a U-shaped positioning frame (405); an end of the fixing plate (401) facing the U-shaped positioning frame (405) is fixedly connected to a connecting column (402); and an end of the connecting column (402) away from the fixing plate (401) is fixedly connected to a limiting chuck (403).

6. A method for cultivating puffballs by using soil-rot pine mycorrhizal fungi as a substitute for raw materials according to claim 5, characterized in that: The U-shaped positioning frame (405) is symmetrically connected to sliding rods (406) on both sides, and the opposite ends of the sliding rods (406) are fixedly connected to inclined blocks (408). The opposite side of the inclined block (408) facing the limiting chuck (403) is set as an inclined surface, and the opposite side of the inclined block (408) away from the limiting chuck (403) is provided with a limiting groove (409). A reset spring (407) is fixedly connected between the inclined block (408) and the U-shaped positioning frame (405), and the reset spring (407) is sleeved on the outer surface of the sliding rod (406).

7. A method for cultivating puffballs by using soil-rot pine mycorrhizal fungi as a substitute for raw materials according to claim 6, characterized in that: The sliding rod (406) is fixedly connected to a movable plate (411) at one end away from the U-shaped positioning frame (405), and one of the movable plates (411) is fixedly connected to a pull rod (410) at one side away from the U-shaped positioning frame (405). The U-shaped positioning frame (405) is symmetrically fixedly connected to support plates (412) on both sides. The support plate (412) is rotatably connected to a central axis (413) at one side away from the first operating frame (301). The outer surface of one end of the central axis (413) away from the support plate (412) is fixedly connected to a central plate (414). The two ends of the central plate (414) are symmetrically rotatably connected to follower plates (415) between the movable plate (411).

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