Explant sterilization treatment device and method for bluegrass tissue culture

Abstract

The invention discloses an explant sterilization treatment device and method for tissue culture of bluegrass, and relates to the field of plant tissue culture. An explant sterilization treatment device for tissue culture of bluegrass comprises a tank body, and also comprises: a cover plate detachably connected to the tank body; a leaky plate, which is lifted and connected to the tank body; the invention, when the pressure of the infusion cylinder changes, the relative distance between the atomizing nozzle and the explant can be automatically adjusted through the linkage of the slider, the pull rope, the spring, the connecting plate and the atomizing nozzle, when the pressure is high, the distance is increased to make the sterilizing agent more evenly dispersed, to prevent local excessive or insufficient sterilization, and to protect the explant from physical damage; when the pressure is low, the distance is shortened to ensure that the sterilizing agent covers the surface of the explant in a concentrated manner, to ensure that all parts can be effectively sterilized, to improve the uniformity and reliability of sterilization, and to help improve the quality of the explant and the success rate of subsequent tissue culture.

Description

Technical Field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an explant sterilization treatment device and method for bluegrass tissue culture. Background Art

[0002] As an important lawn grass species, Poa annua is widely used in garden landscape, sports venues, etc.

[0003] Tissue culture technology is an effective means to quickly propagate excellent varieties of bluegrass, but sterilization of explants is one of the key links in the success of tissue culture.

[0004] Traditional explant sterilization is mostly carried out by spraying sterilizers on the surface of the explants for sterilization. However, traditional spraying methods usually lack precise control means and cannot flexibly adjust the spraying parameters of the sterilizer according to the actual situation of the explants. This easily leads to uneven distribution of the sterilizer. Some areas of the explant may be damaged by excessive chemical action due to excessive spraying of the sterilizer, affecting their normal physiological activity and subsequent growth and development, while some areas may not be effectively killed due to insufficient coverage of the sterilizer. In view of this, the present invention is specially proposed. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide an explant sterilization treatment device and method for bluegrass tissue culture that can overcome the above problems or at least partially solve the above problems.

[0006] In order to solve the above technical problems, the basic concept of the technical solution adopted by the present invention is:

[0007] An explant sterilization treatment device for tissue culture of bluegrass comprises a tank body, and further comprises: a cover plate detachably connected to the tank body; a hole plate, which is lifted and connected to the tank body, wherein a plurality of clamps for fixing explant containers are equidistantly arranged on the circumference of the hole plate; an infusion cylinder, which is fixedly mounted on the cover plate, wherein a slide bar is symmetrically fixedly connected to the cover plate, a connecting plate is slidably connected to the slide bar, the connecting plate is hollow, an atomizing nozzle connected to the internal cavity is fixedly mounted on the connecting plate, and the connecting The disk is located above the leaky disk, the lower end of the infusion cylinder is connected to the cavity inside the connecting disk through the infusion tube, and the infusion cylinder is fixedly connected with a liquid inlet tube; a slider is slidably connected in the infusion cylinder, wherein the slider is provided with a liquid outlet, a guide sleeve is fixedly installed on the infusion cylinder, a pull rope is provided in the guide sleeve, one end of the pull rope is fixedly connected to the connecting disk, and the other end is fixedly connected to the slider, a limiting ring is fixedly connected to the sliding rod, a spring three is sleeved on the sliding rod, and the spring three is located between the limiting ring and the connecting disk.

[0008] Preferably, it also includes a liquid storage tank and a liquid supply pump fixedly installed on the liquid storage tank, the input end of the liquid supply pump is connected to the liquid storage tank, and the output end is fixedly connected to the end of the liquid inlet tube away from the infusion cylinder.

[0009] Preferably, the clamp includes a mounting seat symmetrically fixedly connected to the leaky disk, a guide rod is slidably connected to the mounting seat, one end of the guide rod is fixedly connected to the limiting block, and the other end is fixedly connected to the arc plate, a spring is sleeved on the guide rod, one end of the spring is fixedly connected to the arc plate, and the other end is fixedly connected to the mounting seat.

[0010] In order to facilitate the removal of the used sterilizing agent remaining in the tank body, preferably, a drain pipe is further included, the drain pipe is fixedly connected to the bottom of the tank body, and a valve switch is arranged on the drain pipe.

[0011] In order to facilitate the disassembly and assembly of the cover plate, preferably, it also includes a plurality of fixing bolts, and the cover plate is detachably connected to the tank body through the plurality of fixing bolts.

[0012] Preferably, it also includes a steam generator, which is arranged on one side of the tank body, and the output end of the steam generator is fixedly connected to a gas pipe, and the end of the gas pipe away from the steam generator is connected to the tank body, and a pressure relief pipe connected to the tank body is arranged on the cover plate, and a pressure relief valve is arranged on the pressure relief pipe.

[0013] In order to increase the sealing performance of the connection between the cover plate and the tank body, a sealing ring is further fixedly mounted on the cover plate, and sealing pads that fit the inner wall of the tank body are arranged at both upper and lower ends of the outer wall of the sealing ring.

[0014] In order to further improve the sealing performance of the connection between the cover plate and the tank body, further, an airbag is arranged around the outer wall of the sealing ring, a sealing groove for use with the airbag is opened on the inner wall of the tank body, and a second conduit connected to the airbag and an air release pipe are arranged on the cover plate. The second conduit is used to inflate and pressurize the airbag, and an electromagnetic valve is arranged on the air release pipe.

[0015] In order to automatically push the leak disk and the clamp out of the tank body when the cover is opened, further, a sleeve is fixedly installed on the inner wall of the bottom of the tank body, a piston disk is slidably connected in the sleeve, a push rod is fixedly connected to the piston disk, one end of the push rod passes through the sleeve and is fixedly connected to the leak disk, a spring 2 is arranged in the sleeve, one end of the spring 2 is fixedly connected to the piston disk, and the other end is fixedly connected to the inner wall of the sleeve, and the lower end of the slide rod is against the leak disk; it also includes a conduit 1 fixedly connected to the sleeve, an end of the conduit 1 away from the sleeve extends outside the tank body, an end of the conduit 2 away from the airbag is connected to the sleeve, and a control valve is arranged on the conduit 2.

[0016] The method for sterilizing the explant comprises the following steps:

[0017] Step 1: First, remove the cover installed on the tank body, and the staff can put the container containing the explant into the tank body from the top opening and place it on the leaky plate;

[0018] Step 2: After the container is placed, the container is clamped and fixed by a clamp. After the container is placed and fixed, the cover is reinstalled to make the tank body sealed, and then the explant can be sterilized;

[0019] Step 3: The steam generated by the steam generator is transported into the tank through the gas pipe, so that the steam performs a preliminary high-temperature sterilization treatment on the explants in the tank;

[0020] Step 4: After the high-temperature sterilization process is completed, the liquid supply pump is started. At this time, the liquid supply pump delivers the sterilant in the liquid storage tank into the cavity inside the connecting plate, and finally sprays it on the surface of the explant through the atomizing nozzle, so that it can further sterilize the microorganisms that may remain after the high-temperature sterilization;

[0021] Step 5: When the pressure in the infusion tube is too high, the water flow generates a downward impact force on the slider, and under the action of the impact force, the slider moves downward in the infusion tube. During the movement of the slider, the connecting plate is pulled by the pull rope to drive the atomizing nozzle to move upward, so as to increase the relative distance between the atomizing nozzle and the explant;

[0022] Step 6: When the pressure in the infusion tube is low, the downward impact force of the water flow on the slider is weakened. At this time, the compressed spring 3 generates thrust, pushing the connecting plate to drive the atomizing nozzle downward, thereby shortening the relative distance between the atomizing nozzle and the explant.

[0023] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0024] The present invention uses a steam generator to generate steam to perform preliminary high-temperature sterilization on the explants in the tank, which can kill most microorganisms that are not resistant to high temperatures. Subsequently, a liquid supply pump is used to extract a sterilizing agent for secondary sterilization, which can remove high-temperature resistant microorganisms and microorganisms hidden in places that are difficult for steam to reach, effectively ensuring the sterile state of the explants and improving the success rate of tissue culture.

[0025] When the pressure of the infusion cylinder changes, the relative distance between the atomizing nozzle and the explant can be automatically adjusted through the linkage of the slider, the pull rope, the spring, the connecting plate and the atomizing nozzle. When the pressure is high, the distance is increased to make the sterilizing agent more evenly dispersed, prevent local excessive or insufficient sterilization, and protect the explant from physical damage. When the pressure is low, the distance is shortened to ensure that the sterilizing agent concentrates on covering the surface of the explant, ensure that all parts can be effectively sterilized, improve the uniformity and reliability of sterilization, and be beneficial to improving the quality of the explant and the success rate of subsequent tissue culture.

[0026] The specific implementation modes of the present invention are further described in detail below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In the attached picture:

[0028] Figure 1 It is a schematic diagram of the internal structure of the tank body of the present invention;

[0029] Figure 2 It is a schematic structural diagram of the clamp of the present invention;

[0030] Figure 3 It is a schematic diagram of the internal structure of the sleeve of the present invention;

[0031] Figure 4 It is a schematic diagram of the internal structure of the infusion tube of the present invention;

[0032] Figure 5 The present invention Figure 1 A magnified view of part A;

[0033] Figure 6 It is a front view of the tank body and the steam generator of the present invention.

[0034] In the figure: 1. tank body; 101. cover plate; 102. fixing bolt; 103. leak plate; 2. mounting seat; 201. guide rod; 202. limit block; 203. arc plate; 204. spring one; 3. steam generator; 301. gas pipe; 302. pressure relief pipe; 4. sealing ring; 401. sealing pad; 402. air bag; 5. sleeve; 501. piston plate; 502. push rod; 503. spring two; 504. slide bar; 6. catheter one; 601. catheter two; 602. vent pipe; 7. connecting plate; 701. atomizing nozzle; 702. infusion cylinder; 703. infusion tube; 704. liquid inlet tube; 705. discharge tube; 8. slider; 801. pull rope; 802. spring three; 9. liquid storage tank; 901. liquid supply pump. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The following embodiments are used to illustrate the present invention but are not used to limit the scope of the present invention.

[0036] Example 1: Reference Figure 1 , Figure 2 , Figure 4 , Figure 6 , an explant sterilization treatment device for tissue culture of bluegrass, comprising a tank body 1, and also comprising: a cover plate 101 detachably connected to the tank body 1; a leaky plate 103, which is lifted and connected in the tank body 1, wherein a plurality of clamps for fixing the explant container are equidistantly arranged on the circumference of the leaky plate 103; an infusion cylinder 702, which is fixedly mounted on the cover plate 101, wherein a slide bar 504 is symmetrically fixedly connected to the cover plate 101, a connecting plate 7 is slidably connected to the slide bar 504, the connecting plate 7 is hollow, an atomizing nozzle 701 connected to the internal cavity is fixedly mounted on the connecting plate 7, and the connecting plate 7 is located at the leaky plate 103. Above the hole plate 103, the lower end of the infusion cylinder 702 is connected to the cavity inside the connecting plate 7 through the infusion tube 703, and the infusion cylinder 702 is fixedly connected with a liquid inlet tube 704; the slider 8 is slidably connected in the infusion cylinder 702, wherein the slider 8 is provided with a liquid outlet, and a guide sleeve is fixedly installed on the infusion cylinder 702, and a pull rope 801 is provided in the guide sleeve, one end of the pull rope 801 is fixedly connected to the connecting plate 7, and the other end is fixedly connected to the slider 8, a limit ring is fixedly connected to the slide bar 504, and a spring three 802 is sleeved on the slide bar 504, and the spring three 802 is located between the limit ring and the connecting plate 7.

[0037] It also includes a liquid storage tank 9 and a liquid supply pump 901 fixedly installed on the liquid storage tank 9. The input end of the liquid supply pump 901 is connected to the liquid storage tank 9, and the output end is fixedly connected to the end of the liquid inlet tube 704 away from the infusion cylinder 702.

[0038] It also includes a steam generator 3, which is arranged on one side of the tank body 1. The output end of the steam generator 3 is fixedly connected to a gas pipe 301, and the end of the gas pipe 301 away from the steam generator 3 is connected to the tank body 1. A pressure relief pipe 302 connected to the tank body 1 is arranged on the cover plate 101, and a pressure relief valve is arranged on the pressure relief pipe 302.

[0039] When in use, first remove the cover plate 101 installed on the tank body 1. As the cover plate 101 gradually moves away from the tank body 1, the slide bar 504 fixed on the cover plate 101 and the connection plate 7 slidably connected to the slide bar 504 will follow the movement trajectory of the cover plate 101 and move out of the tank body 1, so that the staff can put the container containing the explant into the tank body 1 from the top opening of the tank body 1 and place it on the leaky plate 103. After the container is placed, the container is clamped and fixed by a clamp to ensure that the container is stably fixed on the leaky plate 103 in subsequent operations and will not be displaced due to the vibration of the device or other external factors.

[0040] After the container is placed and fixed, the cover plate 101 is reinstalled to make the tank body 1 in a sealed state. At this time, the steam generator 3 is turned on, and the steam generated by the steam generator 3 is transported to the tank body 1 through the gas pipe 301. The gas pipe 301 is provided with a one-way valve, so that the explants in the tank body 1 are initially sterilized at high temperature, thereby killing most of the microorganisms that are not resistant to high temperatures. Subsequently, a sterilizing agent is sprayed on the surface of the external body for secondary sterilization, so that some high-temperature resistant microorganisms and microorganisms hidden in the fine structure of the explants that are difficult for steam to reach are removed, and a variety of microorganisms on the surface and inside of the explants are effectively killed, thereby ensuring the sterility of the explants and improving the success rate of tissue culture;

[0041] During the steam sterilization process, when the pressure in the tank body 1 exceeds the pressure relief value set by the pressure relief valve on the pressure relief pipe 302, the pressure relief valve automatically opens to release the pressure and discharge the excess steam to maintain the pressure in the tank body 1 stable, ensuring that the sterilization process is carried out within a safe pressure range, effectively killing a variety of microorganisms on the surface and inside of the explant, ensuring the sterile state of the explant, and improving the success rate of tissue culture.

[0042] When the high-temperature sterilization process is completed, the liquid supply pump 901 is started. At this time, the liquid supply pump 901 extracts the sterilant in the liquid storage tank 9, and the liquid inlet pipe 704 is used as a delivery channel to smoothly deliver the sterilant to the infusion tube 702. Since a liquid outlet is provided on the slider 8 in the infusion tube 702, the sterilant entering the infusion tube 702 flows into the infusion tube 703 connected thereto through the liquid outlet under the action of pressure, and then is delivered to the cavity inside the connecting disk 7 through the infusion tube 703, and finally sprayed on the surface of the explant through the atomizing nozzle 701, so that it can further kill the microorganisms that may remain after high-temperature sterilization, thereby achieving the effect of secondary sterilization, thereby more thoroughly removing the microorganisms on the explant, and providing more reliable sterile explants for subsequent bluegrass tissue culture;

[0043] When the pressure in the infusion tube 702 is too high, the water flow generates a downward impact force on the slider 8. Under the action of the impact force, the slider 8 moves downward in the infusion tube 702. During the movement of the slider 8, the connecting plate 7 is pulled by the pull rope 801 to drive the atomizing nozzle 701 to move upward, and the spring three 802 is compressed to increase the relative distance between the atomizing nozzle 701 and the explant, so that the sprayed sterilant has more sufficient space to diffuse and disperse before reaching the explant, forming a more uniform coverage, preventing the local explant from being over-sterilized and other parts from being under-sterilized due to excessive concentration of local spray, thereby ensuring the consistency of the overall sterilization effect. At the same time, the impact force of the spray is buffered and dispersed when it reaches the explant, reducing direct physical damage to the explant and protecting the integrity of the explant.

[0044] When the pressure in the infusion tube 702 is relatively low, the downward impact force of the water flow on the slider 8 is weakened. At this time, the compressed spring three 802 generates a thrust, pushing the connecting plate 7 to drive the atomizing nozzle 701 to move downward, thereby shortening the relative distance between the atomizing nozzle 701 and the explant, ensuring that the sterilizing agent can cover the surface of the explant more concentratedly, avoiding that some areas cannot be exposed to the sterilizing agent due to the dispersion of the spray, and ensuring that all parts of the explant can be effectively sterilized. At the same time, when the connecting plate 7 moves downward, the pull rope 801 can be used to pull the slider 8 to move upward in the infusion tube 702.

[0045] It should be noted that the liquid inlet pipe 704 is a stretchable and shrinkable hose, so the liquid inlet pipe 704 will not interfere with the disassembly and assembly of the cover plate 101.

[0046] Example 2: Reference Figure 1 , Figure 2 , an explant sterilization treatment device for tissue culture of bluegrass, which is basically the same as Example 1, and further, the clamp includes a mounting seat 2 symmetrically fixedly connected to the leaky plate 103, a guide rod 201 is slidably connected to the mounting seat 2, one end of the guide rod 201 is fixedly connected to the limited block 202, and the other end is fixedly connected to the arc plate 203, a spring 204 is sleeved on the guide rod 201, one end of the spring 204 is fixedly connected to the arc plate 203, and the other end is fixedly connected to the mounting seat 2;

[0047] When the explant container needs to be fixed, the container is placed on the porous plate 103, and the arc plate 203 tightly clamps the container under the elastic force of the spring 204, thereby avoiding the shaking and displacement of the explant container during the sterilization process, ensuring the reliability of the sterilization process, and being beneficial to improving the sterilization quality, reducing the damage to the explant caused by the instability of the container, so as to provide better quality explant materials for subsequent tissue culture. At the same time, the spacing of the arc plate 203 is adaptively adjusted through the elasticity of the spring 204, so that containers of various sizes can be fixed conveniently and quickly, thereby improving the versatility of the clamp.

[0048] It should be noted that the setting of the limit block 202 can prevent the guide rod 201 from escaping from the mounting seat 2, thereby ensuring the stability of the clamp.

[0049] Example 3: Reference Figure 5 , an explant sterilization treatment device for tissue culture of bluegrass, which is basically the same as that of Example 1, and further, a sealing ring 4 is fixedly installed on the cover plate 101, and the upper and lower ends of the outer wall of the sealing ring 4 are provided with sealing pads 401 that fit the inner wall of the tank body 1;

[0050] During steam sterilization, the sealing ring 4 inserted into the tank body 1 squeezes the sealing gasket 401 to fit tightly against the inner wall of the tank body 1, thereby improving the sealing of the connection between the cover plate 101 and the tank body 1 and preventing leakage through the gap between the cover plate 101 and the tank body 1, which would affect the sterilization effect.

[0051] Example 4: Reference Figure 1 , Figure 3 , Figure 5 , an explant sterilization treatment device for tissue culture of bluegrass, which is basically the same as Example 1, and further, an airbag 402 is arranged around the outer wall of the sealing ring 4, and a sealing groove used in conjunction with the airbag 402 is opened on the inner wall of the tank body 1, and a conduit 2 601 and a deflation pipe 602 connected to the airbag 402 are arranged on the cover plate 101, and the conduit 2 601 is used to inflate and pressurize the airbag 402, and an electromagnetic valve is arranged on the deflation pipe 602.

[0052] A sleeve 5 is fixedly installed on the inner wall of the bottom of the tank body 1, and a piston disk 501 is slidably connected in the sleeve 5. A push rod 502 is fixedly connected to the piston disk 501. One end of the push rod 502 passes through the sleeve 5 and is fixedly connected to the leaky disk 103. A spring 2 503 is arranged in the sleeve 5. One end of the spring 2 503 is fixedly connected to the piston disk 501, and the other end is fixedly connected to the inner wall of the sleeve 5. The lower end of the slide rod 504 abuts against the leaky disk 103. It also includes a conduit 1 6 fixedly connected to the sleeve 5, and one end of the conduit 1 6 away from the sleeve 5 extends to the outside of the tank body 1, and the end of the conduit 2 601 away from the airbag 402 is connected to the sleeve 5, and a control valve is arranged on the conduit 2 601.

[0053] When the cover 101 needs to be opened, as the cover 101 gradually moves away from the tank body 1, the slide bar 504 fixed on the cover 101 and the connecting plate 7 slidably connected to the slide bar 504 will follow the movement trajectory of the cover 101 and move out of the tank body 1 to avoid hindering the subsequent rise of the leak plate 103. After that, since the opening of the cover 101 releases the restriction on the spring 2 503, the previously compressed spring 2 503 is no longer bound and begins to release its elastic potential energy, pushing the piston plate 501 to slide upward in the sleeve 5. The upward movement of the piston plate 501 drives the connecting plate 7 fixed thereto to move out of the tank body 1. The push rod 502 moves upward synchronously, and then the push rod 502 pushes the leaky plate 103 upward, and finally pushes the leaky plate 103 out of the tank body 1 until the elastic force of the spring 2 503 and other resistances of the leaky plate 103 reach a balance. At this time, the leaky plate 103 is stabilized at a height that is convenient for operators to place, take out or perform other operations on the explant container, which greatly improves the convenience and efficiency of the operation, reduces the risk of damage to the explant caused by the operator during the operation, and also provides more convenient conditions for the maintenance and cleaning of the device;

[0054] At the same time, as the piston disk 501 moves upward, the volume of the lower space in the sleeve 5 gradually increases, the pressure decreases, and a relatively negative pressure environment is formed. At this time, since the external environment pressure is higher than the pressure in the sleeve 5, under the action of the pressure difference, the external gas is sucked into the sleeve 5 through the conduit 6 to facilitate the subsequent inflation and pressurization of the airbag 402.

[0055] When the cover plate 101 is closed, the cover plate 101 gradually moves closer to the tank body 1 and finally closes the opening at the top of the tank body 1. At this time, the slide bar 504 and the connecting plate 7 will re-enter the tank body 1 as the cover plate 101 moves and return to the initial relative position (such as Figure 1 As shown in the figure), to prepare for the subsequent sterilization operation;

[0056] During the closing process of the cover plate 101, the sliding rod 504 will apply downward pressure to the leaky disk 103, thereby pushing the leaky disk 103 to move downward. At the same time, during the downward movement of the leaky disk 103, the push rod 502 that moves downward synchronously can push the piston disk 501 to move downward in the sleeve 5 and compress the spring 2 503. At the same time, as the piston disk 501 moves downward, the gas stored in the sleeve 5 is gradually compressed until the cover plate 101 closes the opening at the top of the tank body 1. At this time, by opening the control valve on the conduit 2 601, the compressed gas can be quickly filled into the airbag 402 through the conduit 2 601, so that the airbag 402 expands and fits tightly with the sealing groove, further improving the sealing performance of the connection between the cover plate 101 and the tank body 1, effectively preventing steam leakage during steam sterilization of the explant, and then maintaining the required sterilization pressure and temperature in the tank body 1, thereby improving the work efficiency and quality of the explant sterilization process;

[0057] It should be noted that both conduit 1 6 and conduit 2 601 are provided with one-way valves, and conduit 2 601 adopts a stretchable and shrinkable hose, so conduit 2 601 will not interfere with the disassembly and assembly of the cover plate 101.

[0058] Example 5: Reference Figure 1 , an explant sterilization treatment device for tissue culture of bluegrass, which is basically the same as that of Example 1, and further includes a drainage pipe 705, the drainage pipe 705 is fixedly connected to the bottom of the tank body 1, and a valve switch is provided on the drainage pipe 705;

[0059] After completing the sterilization spraying or steam sterilization, the residual liquid in the tank body 1 (such as sterilization waste liquid, condensed water, etc.) will gather at the bottom of the tank body 1 under the action of gravity. At this time, by opening the valve switch on the drain pipe 705, the liquid can be discharged from the tank body 1, thereby keeping the inside of the tank body 1 clean and hygienic, preventing the residual liquid from interfering with or contaminating the subsequent sterilization operation, ensuring that the environment of each sterilization treatment is relatively stable and pure, and at the same time, avoiding the accumulation of residual liquid in the tank body 1 to cause bacterial growth and other problems, reducing the risk of the explant being contaminated again.

[0060] Example 6: Reference Figure 1 , Figure 6 , an explant sterilization treatment device for tissue culture of bluegrass, which is basically the same as that of Example 1, and further comprises a plurality of fixing bolts 102, and the cover plate 101 is detachably connected to the tank body 1 through the plurality of fixing bolts 102;

[0061] Since the cover plate 101 is detachably connected to the tank body 1 by a plurality of fixing bolts 102, when the tank body 1 needs to be opened for operation (such as placing an explant or cleaning the interior), the cover plate 101 can be removed by unscrewing the fixing bolts 102. After the operation is completed, the cover plate 101 is firmly mounted on the tank body 1 by the fixing bolts 102 to ensure the sealing of the tank body 1.

[0062] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as above with a preferred embodiment, it is not used to limit the present invention.

Claims

1. A sterilization treatment device for explants used for tissue culture of bluegrass, comprising a tank (1), characterized in that: Also includes: Removing the cover plate (101) connected to the tank body (1); The leak plate (103) is connected in a lifting manner in the tank body (1). Wherein, a plurality of clamps for fixing the explant container are arranged at equal intervals on the circumference of the leaky plate (103); The infusion cylinder (702) is fixedly mounted on the cover plate (101). The cover plate (101) is symmetrically fixedly connected with a slide bar (504), the slide bar (504) is slidably connected with a connection plate (7), the connection plate (7) is hollow, an atomizing nozzle (701) connected to the internal cavity is fixedly installed on the connection plate (7), the connection plate (7) is located above the leak plate (103), the lower end of the infusion cylinder (702) is connected to the cavity inside the connection plate (7) through the infusion tube (703), and the infusion cylinder (702) is fixedly connected with a liquid inlet tube (704); The slider (8) is slidably connected in the infusion tube (702). The slider (8) is provided with a liquid outlet, the infusion tube (702) is fixedly provided with a guide sleeve, a pull rope (801) is provided in the guide sleeve, one end of the pull rope (801) is fixedly connected to the connection disk (7), and the other end is fixedly connected to the slider (8), the slide bar (504) is fixedly connected with a limit ring, the slide bar (504) is sleeved with a spring three (802), and the spring three (802) is located between the limit ring and the connection disk (7); and the liquid storage tank (9) and a liquid supply pump (901) fixedly installed on the liquid storage tank (9), the liquid supply pump (901) is provided with a liquid supply pump (901) fixedly installed on the liquid storage tank (9), and the liquid supply pump (901) is provided with a liquid supply pump (901) fixedly installed on the liquid storage tank (9). The liquid pump (901) has an input end connected to the liquid storage tank (9), and an output end fixedly connected to an end of a liquid inlet pipe (704) away from the infusion cylinder (702); and further comprises a steam generator (3), the steam generator (3) being arranged on one side of the tank body (1); the output end of the steam generator (3) being fixedly connected to an air supply pipe (301); an end of the air supply pipe (301) away from the steam generator (3) being connected to the tank body (1); a pressure relief pipe (302) connected to the tank body (1) being arranged on the cover plate (101); and a pressure relief valve being arranged on the pressure relief pipe (302).

2. The explant sterilization treatment device for tissue culture of bluegrass according to claim 1, characterized in that: The clamp comprises a mounting seat (2) symmetrically fixedly connected to the leaky disk (103); a guide rod (201) is slidably connected to the mounting seat (2); one end of the guide rod (201) is fixedly connected to a limit block (202), and the other end is fixedly connected to an arc plate (203); a spring 1 (204) is sleeved on the guide rod (201); one end of the spring 1 (204) is fixedly connected to the arc plate (203), and the other end is fixedly connected to the mounting seat (2).

3. The explant sterilization treatment device for tissue culture of bluegrass according to claim 1, characterized in that: It also comprises a liquid discharge pipe (705), the liquid discharge pipe (705) being fixedly connected to the bottom of the tank body (1), and a valve switch being arranged on the liquid discharge pipe (705).

4. The explant sterilization treatment device for tissue culture of bluegrass according to claim 1, characterized in that: It also comprises a plurality of fixing bolts (102), and the cover plate (101) is detachably connected to the tank body (1) via the plurality of fixing bolts (102).

5. The explant sterilization treatment device for tissue culture of bluegrass according to claim 1, characterized in that: A sealing ring (4) is fixedly mounted on the cover plate (101), and sealing pads (401) that fit against the inner wall of the tank body (1) are provided at both upper and lower ends of the outer wall of the sealing ring (4).

6. The explant sterilization treatment device for tissue culture of bluegrass according to claim 5, characterized in that: An air bag (402) is arranged around the outer wall of the sealing ring (4), a sealing groove for use with the air bag (402) is provided on the inner wall of the tank body (1), a second conduit (601) and an air release pipe (602) connected to the air bag (402) are arranged on the cover plate (101), the second conduit (601) is used to inflate and pressurize the air bag (402), and a solenoid valve is arranged on the air release pipe (602).

7. The explant sterilization treatment device for tissue culture of bluegrass according to claim 6, characterized in that: A sleeve (5) is fixedly mounted on the inner wall of the bottom of the tank body (1), a piston disc (501) is slidably connected inside the sleeve (5), a push rod (502) is fixedly connected to the piston disc (501), one end of the push rod (502) passes through the sleeve (5) and is fixedly connected to the leaky disc (103), a spring 2 (503) is arranged inside the sleeve (5), one end of the spring 2 (503) is fixedly connected to the piston disc (501), and the other end is fixedly connected to the inner wall of the sleeve (5), and the lower end of the sliding rod (504) abuts against the leaky disc (103); It also includes a conduit 1 (6) fixedly connected to the sleeve (5), wherein one end of the conduit 1 (6) away from the sleeve (5) extends outside the tank body (1), and one end of the conduit 2 (601) away from the airbag (402) is connected to the sleeve (5), and a control valve is provided on the conduit 2 (601).

8. A method for sterilizing explants, using the explant sterilizing device for tissue culture of bluegrass as claimed in claim 1, characterized in that: The steps include: Step 1: first remove the cover plate (101) installed on the tank body (1), and the staff can then put the container containing the explant into the tank body (1) from the top opening and place it on the leak plate (103); Step 2: After the container is placed, the container is clamped and fixed by a clamp. After the container is placed and fixed, the cover plate (101) is reinstalled to make the tank body (1) in a sealed state, and then the explant can be sterilized; Step 3: The steam generated by the steam generator (3) is transported into the tank body (1) through the gas pipe (301), so that the steam performs a preliminary high-temperature sterilization treatment on the explants in the tank body (1); Step 4: After the high-temperature sterilization process is completed, the liquid supply pump 901 delivers the sterilant in the liquid storage tank 9 into the cavity inside the connecting disk 7, and finally sprays it on the surface of the explant through the atomizing nozzle 701, so that it can further sterilize the microorganisms that may remain after the high-temperature sterilization; Step 5: When the pressure in the infusion tube (702) is too high, the water flow generates a downward impact force on the slider (8). Under the action of the impact force, the slider (8) moves downward in the infusion tube (702). During the movement of the slider (8), the connecting plate (7) is pulled by the pull rope (801) to drive the atomizing nozzle (701) to move upward, thereby increasing the relative distance between the atomizing nozzle (701) and the explant; Step 6: When the pressure in the infusion tube (702) is low, the downward impact force exerted by the water flow on the slider (8) is weakened. At this time, the compressed spring three (802) generates a thrust, which pushes the connecting plate (7) to drive the atomizing nozzle (701) to move downward, thereby shortening the relative distance between the atomizing nozzle (701) and the explant.

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