Ergothioneine anti-aging functional composition

Abstract

The invention discloses an ergothioneine composition with an anti-aging function, and relates to the field of health-care foods, and the composition has the effects of resisting aging and cell molecule oxidation reaction. The composition disclosed by the invention is prepared from the following composition components: ergothioneine (0.162 to 0.185), a wheat germ extract (0.063 to 0.085), coenzyme Q10 (0.042 to 0.063), quercetin (0.35 to 0.55), a turmeric extract (0.032 to 0.054), a black pepper extract (0.003 to 0.006) and aminobutyric acid (0.205 to 0.227). The composition is used for assisting body functions in eliminating lipid, protein, DNA (deoxyribonucleic acid) and the like in cells damaged by oxidative stress in a body. The composition is prepared into equivalent particles by fully and physically stirring and uniformly mixing, is convenient for human body to absorb, and can remarkably improve the antioxidant capacity and delay the aging process after being taken for a long time. The composition not only can effectively resist oxidative stress disoperation, but also can promote cell repair. The raw materials of the composition can be purchased in the market, and the composition is high in production benefit and beneficial to wide application.

Description

Technical Field

[0001] The invention relates to an ergothioneine anti-aging functional composition, and in particular to the field of health food anti-aging. Background Art

[0002] As age increases, human body functions gradually decline, and free radicals increase, resulting in accelerated cell damage. The human body's ability to eliminate free radicals in body functions decreases, thereby causing a variety of aging symptoms. With the development of society, the pursuit of health and skin care topics is also increasing day by day. The most widely used method is to take in food, select food containing corresponding trace elements, and take in less elements required by the body. This method lacks a standard on the required amount of elements for the body, and the trace elements contained in each food are not comprehensive. The present invention is directed to the anti-aging and anti-oxidation ingredients required by the human body by scientifically proportioning the obtained ergothioneine anti-aging composition, effectively improving the antioxidant level in the body, slowing down the cell aging process, thereby achieving the effect of delaying aging, repairing cell damage, and improving body functions. Summary of the invention

[0003] The object of the present invention is to provide an ergothioneine anti-aging functional composition, wherein the ergothioneine anti-aging functional composition is mainly prepared by fully mixing and stirring ergothioneine, wheat germ extract, coenzyme Q10, quercetin, turmeric extract, black pepper extract and aminobutyric acid in a stirring pot, wherein the multiple components complement each other, effectively assist the synergistic effect of the human body, enhance the antioxidant effect, accurately remove free radicals, promote cell regeneration, comprehensively improve body functions, and achieve the effects of anti-aging and promoting cell repair.

[0004] Through long-term experiments, it was found that the ergothioneine anti-aging functional composition is mainly composed of the following proportions: ergothioneine (0.162-0.185), wheat germ extract (0.063-0.085), coenzyme Q10 (0.042-0.063), quercetin (0.35-0.55), turmeric extract (0.032-0.054), black pepper extract (0.003-0.006), and aminobutyric acid (0.205-0.227). These compositions have the best anti-aging effect in assisting the human body to clear the body's daily metabolism and reduce ergothioneine. Experimental data show that after taking this formula, the activity of antioxidant enzymes in the body is significantly improved, and the cell damage rate is reduced by 30%, effectively delaying aging and repairing damaged cells.

[0005] The composition of the present invention has no special instructions for the raw materials used, and all of them can be obtained through conventional market purchase channels: ergothioneine is purchased from Sichuan Cullinan Technology Co., Ltd., corresponding to the brand name Cullinan; wheat germ extract is purchased from Jinriheye (Xiamen) Food Technology Co., Ltd., corresponding to the brand Jinriheye GOLDKANO; coenzyme Q10 is purchased from Xi'an Lavia Biotechnology Co., Ltd., corresponding to the brand name Qin Weiya; quercetin is purchased from Chengdu Desite Biotechnology Co., Ltd., corresponding to the brand Desite Bio DESITE; turmeric extract is purchased from Xi'an Tianfeng Biotechnology Co., Ltd., corresponding to the brand NFPREMIX; black pepper extract is purchased from Xi'an Tianben Bioengineering Co., Ltd., corresponding to the brand PHYTO NUTRITIONOR; aminobutyric acid is purchased from Shenzhen Jinfuyuan Biotechnology Co., Ltd., corresponding to the brand Jinfuyuan.

[0006] To achieve the above object, the present invention provides an ergothioneine anti-aging functional composition, comprising the following embodiment ingredients: ergothioneine, wheat germ extract, coenzyme Q10, quercetin, turmeric extract, black pepper extract, and aminobutyric acid. The corresponding embodiment ratios are (0.153-0.198), (0.045-0.098), (0.033-0.078), (0.32-0.65), (0.025-0.068), (0.001-0.007), and (0.192-0.239).

[0007] The embodiment methods of the ergothioneine and other compositions are as follows:

[0008] Further, the embodiment has the following proportions: ergothioneine (0.153), wheat germ extract (0.045), coenzyme Q10 (0.033), quercetin (0.32), turmeric extract (0.025), black pepper extract (0.001), and aminobutyric acid (0.192), and the fluorescence intensity P value is detected to be <0.05.

[0009] Further, the embodiment comprises the following components: ergothioneine (0.156), wheat germ extract (0.048), coenzyme Q10 (0.038), quercetin (0.33), turmeric extract (0.028), black pepper extract (0.002), and aminobutyric acid (0.198), and the fluorescence intensity P value is detected to be <0.05.

[0010] Furthermore, the proportions of the embodiment are as follows: ergothioneine (0.16), wheat germ extract (0.054), coenzyme Q10 (0.04), quercetin (0.35), turmeric extract (0.03), black pepper extract (0.003), and aminobutyric acid (0.202), and the fluorescence intensity P value is detected to be <0.04.

[0011] Further, the embodiment has the following proportions: ergothioneine (0.162), wheat germ extract (0.063), coenzyme Q10 (0.042), quercetin (0.35), turmeric extract (0.032), black pepper extract (0.003), and aminobutyric acid (0.205), and the fluorescence intensity P value is detected to be <0.02.

[0012] Further, the embodiment comprises the following components: ergothioneine (0.174), wheat germ extract (0.075), coenzyme Q10 (0.054), quercetin (0.52), turmeric extract (0.045), black pepper extract (0.003), and aminobutyric acid (0.218), and the fluorescence intensity P value is detected to be <0.01.

[0013] Furthermore, the proportions of the embodiment are as follows: ergothioneine (0.185), wheat germ extract (0.085), coenzyme Q10 (0.063), quercetin (0.55), turmeric extract (0.054), black pepper extract (0.006), and aminobutyric acid (0.227), and the fluorescence intensity P value is detected to be <0.02.

[0014] Further, the embodiment comprises the following components: ergothioneine (0.198), wheat germ extract (0.098), coenzyme Q10 (0.078), quercetin (0.65), turmeric extract (0.068), black pepper extract (0.007), and aminobutyric acid (0.239), and the fluorescence intensity P value is detected to be <0.04.

[0015] The corresponding implementation data table of the ergothioneine anti-aging functional composition is shown in Table 1 below.

[0016] The ergothioneine anti-aging functional composition has the following ingredient ratios: ergothioneine, wheat germ extract, coenzyme Q10, quercetin, turmeric extract, black pepper extract, and aminobutyric acid can achieve optimal anti-aging and cell damage repair functions within the following ratios (0.58-0.65): (0.015-0.024): (0.076-0.085): (0.094-0.11): (0.092-0.115): (0.09-0.118). Specific implementation plan

[0018] The specific implementation plan selected zebrafish as the experimental subject. The zebrafish has a genome similarity of up to 87% with the human genome, which is suitable as an experimental subject. Zebrafish showed a significant response to the ergothioneine anti-aging composition in the experiment, and its cell repair function was significantly improved. By adjusting the proportion of ingredients, scientists observed that the physiological indicators of zebrafish were significantly improved, verifying the anti-aging effect of the composition. Experimental data showed that within the optimal ratio range, the survival rate and health status of zebrafish were better than those of the control group, further confirming the application potential of the composition. The results of the zebrafish experiment showed that the ergothioneine anti-aging composition can effectively promote cell regeneration and delay the aging process at a specific ratio. Its antioxidant and repair mechanisms have important reference value for human anti-aging research, and lay a solid foundation for the future development of efficient anti-aging products.

[0019] The zebrafish selected were healthy adult fish that were fertilized 3 days ago, and the experimental environment was strictly controlled to ensure the accuracy and reliability of the data.

[0020] The experimental detection instruments and reagents include: stereo fluorescence microscope (SZX7, Olympus, Japan), ROS detection kit, glutathione (GSH) detection kit, SOD activity detection kit, MTT cell proliferation detection kit. The experimental steps are strictly carried out according to the standardized process to ensure the reproducibility of the results.

[0021] The experimental object zebrafish was divided into seven groups during the experiment, and each group was divided into three groups, respectively named: blank control group, positive control group and sample treatment group.

[0022] The blank control group was not treated in any way, the positive control group was treated with glutathione, and a certain proportion of ergothioneine anti-aging functional composition solution was added to the sample treatment group. After a period of culture, observation by stereo fluorescence microscopy showed that the cell viability of zebrafish in the sample treatment group was significantly enhanced, the ROS level was significantly reduced, the GSH and SOD activities were significantly increased, and the MTT test showed that the cell proliferation rate was greatly increased, which further verified the effectiveness of the ergothioneine anti-aging functional composition.

[0023] Through comparative analysis, it was found that the physiological indicators and survival status of zebrafish in the sample treatment group were significantly better than those in other groups. At the same time, compared with the blank control group, the fluorescence intensity of the sample treatment group was significantly reduced (P<0.05), indicating that the composition has anti-aging effects.

[0024] In Example 1, by the above experimental method, a sample with a maximum safety value of 500 μg / mL concentration was taken after the composition containing ergothioneine (0.153), wheat germ extract (0.045), coenzyme Q10 (0.033), quercetin (0.32), turmeric extract (0.025), black pepper extract (0.001), and aminobutyric acid (0.192) was dissolved in water, and the fluorescence intensity of the experimental data was compared with the blank control group P value <0.05.

[0025] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0026] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0027] In Example 2, by the above experimental method, a sample with a maximum safety value of 500 μg / mL concentration was taken after the composition containing ergothioneine (0.156), wheat germ extract (0.048), coenzyme Q10 (0.038), quercetin (0.33), turmeric extract (0.028), black pepper extract (0.002), and aminobutyric acid (0.198) was dissolved in water, and the fluorescence intensity of the experimental data was compared with the blank control group P value <0.05.

[0028] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0029] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0030] In Example 3, by the above experimental method, a sample with a maximum safety value of 500 μg / mL concentration was taken after the composition containing ergothioneine (0.16), wheat germ extract (0.054), coenzyme Q10 (0.04), quercetin (0.35), turmeric extract (0.03), black pepper extract (0.003), and aminobutyric acid (0.202) was dissolved in water, and the fluorescence intensity of the experimental data was compared with the blank control group P value <0.04.

[0031] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0032] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0033] In Example 4, by the above experimental method, the corresponding composition containing ergothioneine (0.162), wheat germ extract (0.063), coenzyme Q10 (0.042), quercetin (0.35), turmeric extract (0.032), black pepper extract (0.003), and aminobutyric acid (0.205) was dissolved in water and a sample with a maximum safety value of 500 μg / mL concentration was taken. The fluorescence intensity of the experimental data was compared with the blank control group P value <0.02.

[0034] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0035] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0036] In Example 5, by the above experimental method, the corresponding composition containing ergothioneine (0.174), wheat germ extract (0.075), coenzyme Q10 (0.054), quercetin (0.52), turmeric extract (0.045), black pepper extract (0.003), and aminobutyric acid (0.218) was dissolved in water and a sample with a maximum safety value of 500 μg / mL concentration was taken. The fluorescence intensity of the experimental data was compared with the blank control group P value <0.01.

[0037] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0038] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0039] In Example 6, by the above experimental method, the corresponding composition containing ergothioneine (0.185), wheat germ extract (0.085), coenzyme Q10 (0.063), quercetin (0.55), turmeric extract (0.054), black pepper extract (0.006), and aminobutyric acid (0.227) was dissolved in water and a sample with a maximum safety value of 500 μg / mL concentration was taken. The fluorescence intensity of the experimental data was compared with the blank control group P value <0.02.

[0040] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0041] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0042] In Example 7, by the above experimental method, the corresponding composition containing ergothioneine (0.198), wheat germ extract (0.098), coenzyme Q10 (0.078), quercetin (0.65), turmeric extract (0.068), black pepper extract (0.007), and aminobutyric acid (0.239) was dissolved in water and a sample with a maximum safety value of 500 μg / mL concentration was taken. The fluorescence intensity of the experimental data was compared with the blank control group P value <0.04.

[0043] In addition, experiments have found that the combination is effective in improving zebrafish skin elasticity and reducing wrinkles.

[0044] It was further demonstrated that the composition increased the activity of antioxidant enzymes in zebrafish and reduced oxidative stress damage, indicating that the composition had anti-aging effects.

[0045] In the above experiments, the sample treatment group with the maximum safety value of 500 μg / mL concentration was used as the blank control group, and the experimental controls of each group are shown in Table 2.

[0046] Data analysis and processing:

[0047] The data analysis was mainly performed using SPSS26 software, and GraphPad Prism8 was used for drawing. The data statistics were expressed as mean ± standard deviation. The experimental results were tested for normal distribution using SPSS software. If the data conformed to the normal distribution, the T parameter test or analysis of variance (ANOVA) was used for significance. If it did not conform to the normal distribution, a non-parametric test was used.

[0048] The corresponding numerical comparison results produced by the experiment show that the fluorescence intensity of the sample treatment group is significantly reduced or absent when compared with the blank control group, indicating that the test samples in the sample treatment group have obvious anti-aging and antioxidant functions.

[0049] The experiment was performed by statistically analyzing the average ROS fluorescence intensity as shown in Table 3:

[0050] The test results show that the ergothioneine anti-aging functional composition disclosed in the present invention has good anti-aging effects in Experimental Examples 1-7, especially Experimental Examples 4-6. It can be seen that through the experimental cases implemented above, the ergothioneine anti-aging functional composition disclosed in the present invention corresponds to ergothioneine (0.162-0.185), wheat germ extract (0.063-0.085), coenzyme Q10 (0.042-0.063), quercetin (0.35-0.55), turmeric extract (0.032-0.054), black pepper extract (0.003-0.006), and aminobutyric acid (0.205-0.227) composition range ratio embodiment has the best effect.

[0051] Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified in proportion or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

[0052] Table 1: Serial number Ergothioneine Wheat Germ Extract Coenzyme Q10 Quercetin Turmeric Extract Black pepper extract GABA Corresponding P value 1 0.153 0.045 0.033 0.32 0.025 0.001 0.192 <0.05 2 0.156 0.048 0.038 0.33 0.028 0.002 0.198 <0.05 3 0.16 0.054 0.04 0.35 0.03 0.003 0.202 <0.04 4 0.162 0.063 0.042 0.35 0.032 0.003 0.205 <0.02 5 0.174 0.075 0.054 0.52 0.045 0.003 0.218 <0.01 6 0.185 0.085 0.063 0.55 0.054 0.006 0.227 <0.02 7 0.198 0.098 0.078 0.65 0.068 0.007 0.239 <0.04 Table 2: Test items Detection concentration (μg / mL) P-value Test results Anti-aging efficacy test 1 500 P＜0.05 efficient Anti-aging efficacy test 2 500 P＜0.05 efficient Anti-aging efficacy test 3 500 P＜0.04 efficient Anti-aging efficacy test 4 500 P＜0.02 obvious Anti-aging efficacy test 5 500 P＜0.01 obvious Anti-aging efficacy test6 500 P＜0.02 obvious Anti-aging efficacy test7 500 P＜0.04 efficient Table 3: Serial number Blank control group Positive control group Sample processing group Example 1 62.15±32.79 22.03±13.82 26.25±8.21 Example 2 63.22±32.55 22.52±13.53 26.62±8.69 Example 3 61.68±31.45 22.87±13.92 26.43±8.74 Example 4 61.85±32.35 23.53±13.75 26.37±7.44 Example 5 62.23±31.75 21.75±14.48 25.33±7.25 Example 6 62.22±32.22 22.83±13.86 26.39±7.68 Example 7 61.96±31.85 23.58±13.78 26.32±8.78

Claims

1. a thioneine anti-aging functional composition, the thioneine anti-aging functional composition main component is coordinated by thioneine, wheat germ extract, coenzyme Q10, quercetin, turmeric extract (0.032-0.054), black pepper extract, and aminobutyric acid, and the composition of relative proportion is evenly formed by physical stirring and mixing. The composition plays a multi-level antioxidant effect in vivo, effectively removing the free radicals produced by oxidative stress.

2. In the ergothioneine anti-aging functional composition: the corresponding proportions of ergothioneine, wheat germ extract, coenzyme Q10, quercetin, turmeric extract, black pepper extract, and aminobutyric acid are (0.162-0.185): (0.063-0.085): (0.042-0.063): (0.35-0.55): (0.032-0.054): ((0.003-0.006): (0.205-0.227); the combination ratio is evenly mixed by sufficient physical stirring to obtain a composition with an optimal ratio.

3. in the ergothioneine anti-aging functional composition formula according to claim, wherein Ergothioneine, the molecular weight of which is less than 6000Da.

4. in the ergothioneine anti-aging functional composition according to claim 1, it is characterized in that Any one or more synergistic combinations of 1-7 ingredients can help the body eliminate free radicals and promote cell repair.

5. The ergothioneine anti-aging functional composition according to claim 1, wherein: The anti-aging composition accounts for 85%-90% of the total ingredients.

6. according to the anti-aging composition of ergothioneine according to claim, a certain proportion of compositions is evenly deployed into the granule composition containing corresponding component by mixing and stirring, and the compositions can also be made into corresponding tablets, capsules, granules, pills or oral liquids, so that different groups of people can select suitable taking form according to individual needs.