Enzymolysis lotus seed tablet for promoting digestion and release of curcumin and preparation method of enzymolysis lotus seed tablet

Abstract

The invention belongs to the technical field of food processing, and particularly relates to an enzymolysis lotus seed tablet for promoting digestion and release of curcumin and a preparation method of the enzymolysis lotus seed tablet. The curcumin-lotus seed based tablet disclosed by the invention is prepared from the following raw materials: curcumin, lotus seed powder and enzyme; the preparation method comprises the following steps: dispersing the lotus seed powder in water, performing high-speed shearing, adding alpha-amylase, and performing enzymolysis to obtain lotus seed slurry; mixing the lotus seed powder with a curcumin solution, drying to prepare curcumin-lotus seed composite powder, and finally tabletting to prepare the curcumin-lotus seed tablet. The tablet disclosed by the invention is simple in ingredients, free of addition of auxiliary materials and capable of meeting the requirements of people on label cleaning; meanwhile, the enzyme modified lotus seed powder is used as an embedding medium of curcumin, can exert the medicinal and edible effects of lotus seeds, such as heart nourishing and blood pressure lowering, while being used as an embedding wall material, and is a natural and safe plant-based delivery carrier. The tablet provided by the invention is safe and green, and is a convenient, healthy and high-quality tablet health product.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and particularly relates to an enzymatic lotus seed tablet for promoting the digestion and release of curcumin and a preparation method thereof. Background Art

[0002] Modern life and work are fast-paced and stressful, and consumers' demand for tablet-type nutritional supplements continues to increase. At the same time, the development of food raw materials with medicinal and edible properties is endless, and using food raw materials with medicinal and edible properties as the formula of health care tablets will help improve the nutritional quality of the products. At present, there are few reports on the research of tablets with medicinal and edible properties.

[0003] Lotus seed, also known as lotus root and lotus seed, has the effects of nourishing the heart and calming the mind, strengthening the heart and lowering blood pressure, and has a dietary therapeutic effect in terms of lowering blood sugar, anti-oxidation, anti-aging, immunomodulation, liver protection, regulating intestinal flora, etc. There are rich categories of lotus seeds, and according to regional differences, there are mainly Jianlian, Ganlian, Xuanlian, Xianglian, etc. Zheng Baodong et al. (Morrison WR, Gadan H. 1987. The amylose and lipid contents of starch granules in developing wheat endosperm. Journal of Cereal Science, 5: 263-275.) analyzed the components of the main lotus seeds in China and found that lotus seeds are rich in protein and polysaccharides. Although lotus seeds have rich potential nutritional value, there are few reports based on the application of lotus seed powder in tablets.

[0004] Curcumin is a polyphenol compound with a diketone structure isolated from the rhizome of the ginger family. It is one of the widely used natural active substances. As a food additive, curcumin also has multiple biological activities such as anti-inflammatory, antibacterial, antioxidant (anti-aging), and anti-tumor. Due to its high safety, low side effects, low price and easy availability, the importance of curcumin in food nutrition and medicine has gradually become prominent, and its biological effects have therefore become one of the research hotspots in recent years. Curcumin has multiple physiological and pharmacological activities and extremely high safety, and can be used to treat and prevent a variety of human diseases, but when it is used as a nutritional health product formulation, there is a problem of low oral bioavailability, which limits its application. Wooster et al. (Wooster TJ, Acquistapace S, Mettraux C, et al. Hierarchically structured phase separated biopolymer hydrogels create tailorable delayed burst release during gastrointestinal digestion [J]. Journal of Colloid and Interface Science, 2019, 553: 308-319.) believe that controlled release can achieve a higher concentration of functional factors in the gastrointestinal tract, thereby ensuring that it produces a strong reaction and maximum absorption at a specific time or position, thereby improving its physiological activity. In addition, improving the solubility of curcumin in the carrier can also significantly improve the bioacceptability of curcumin (Yu HL, Shi K, Liu D, et al. Development of a food-grade organogel with high bioaccessibility and loading of curcuminoids [J]. Food Chemistry, 2012, 131 (1): 48-54.). However, curcumin is dissolved in lotus seed powder solution, and whether it can be fully dissolved and mixed to form a stable solution, and then obtain powder tableting is a problem currently faced.

[0005] Enzymatic modification refers to a method in which starch granules interact with enzyme binding sites to form holes and other surface structural changes in starch granules from the outside to the inside. Enzyme catalysis has mild reaction conditions, high catalytic efficiency, and good substrate specificity. The more commonly used enzymes are: α-amylase, β-amylase, pullulanase, glucoamylase, etc. It can be seen that enzyme treatment is an effective way to improve the solubility of lotus seed powder, which is expected to enable it to be compounded with curcumin, and then used in the field of health care tableting, and as a carrier to achieve effective delivery of polyphenols. Summary of the invention

[0006] In order to overcome the deficiencies of the above-mentioned prior art, the present invention uses enzyme-modified lotus seed powder as an embedding agent for curcumin to prepare curcumin-lotus seed-based tablets, which can not only exert the medicinal and edible effects of lotus seeds, but also promote the digestion and release of curcumin. The tablet is a convenient, healthy and high-quality tablet health product.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] The first aspect of the present invention provides a curcumin lotus seed tablet. The raw materials of the curcumin lotus seed tablet include curcumin, lotus seed powder and alpha-amylase. The mass ratio of the curcumin to the lotus seed powder is 1:400-600, and the amount of the alpha-amylase is 0.5%-2.5% of the mass of the lotus seed powder.

[0009] The second aspect of the present invention provides a method for preparing the curcumin lotus seed tablets according to the first aspect, comprising the following steps:

[0010] S1, dispersing lotus seed powder in water, stirring at high speed, adding α-amylase, and obtaining lotus seed slurry after enzymolysis;

[0011] S2, dissolving curcumin in an organic solvent to prepare a curcumin solution, and then mixing the curcumin solution with the lotus seed slurry of step S1, and drying the obtained mixed slurry to obtain a curcumin-lotus seed composite powder, and then tableting the composite powder to obtain a curcumin lotus seed tablet.

[0012] The tablet of the present invention has simple ingredients and no auxiliary materials are added, which meets people's needs for clean labels; at the same time, the enzyme-modified lotus seed powder is used as an embedding agent for curcumin. While being used as an embedding wall material, it can exert the medicinal and edible effects of lotus seeds, such as nourishing the heart and lowering blood pressure, and is a natural and safe plant-based delivery carrier. The tablet of the present invention is safe and green, and is a convenient, healthy, and high-quality tablet health product.

[0013] Preferably, the α-amylase in S1 is a thermostable α-amylase, which is prepared by fermentation, extraction and purification of Bacillus licheniformis, and the activity of the thermostable α-amylase is 40,000 U / g.

[0014] Preferably, the enzymatic hydrolysis in S1 is water bath enzymatic hydrolysis at a temperature of 50-60° C. for 5-7 h.

[0015] Preferably, the high-speed shear stirring in S1 is followed by high-speed shear stirring at a speed of 11000-15000 rpm for 1-5 min.

[0016] Preferably, S1 disperses lotus seed powder in water to prepare a powder dispersion with a mass concentration (w / v) of 20-30%.

[0017] Preferably, in S2, after the curcumin-lotus seed composite powder is obtained, it is vacuum-sealed (to prevent the powder from absorbing moisture) and stored frozen at -20°C (to avoid decomposition of curcumin at room temperature).

[0018] Preferably, S2 dissolves curcumin in an organic solvent to prepare a curcumin solution with a mass concentration (w / v) of 0.2-0.5%.

[0019] Preferably, the drying in S2 is pre-freezing at -20°C for 10-15h and then freeze-drying at -80°C for 70-80h to prepare freeze-dried powder.

[0020] Preferably, the organic solvent in S2 comprises ethanol.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention discloses a lotus seed tablet for promoting the digestion and release of curcumin and a preparation method thereof. The raw materials for preparing the curcumin-lotus seed-based tablet include curcumin, lotus seed powder and enzyme; the preparation method comprises first dispersing the lotus seed powder in water, adding a-amylase after high-speed shearing, and obtaining lotus seed slurry after enzymolysis; then mixing the lotus seed slurry with a curcumin solution, drying to obtain a curcumin-lotus seed composite powder, and finally tableting to obtain a curcumin lotus seed tablet. Specifically, the present invention has the following advantages:

[0023] (1) The tablet formula is simple, no excipients are added, the clean label feature is prominent, and the tablet can be prepared and formed without adding excipients.

[0024] (2) The enzymatic hydrolysis method was used to cleverly, effectively, and significantly improve the solubility of lotus seed powder, allowing lotus seed powder and curcumin to be effectively and stably combined.

[0025] (3) After water-bath gelatinization, the compressibility of lotus seed powder is greatly improved, far exceeding the pharmacopoeia's requirements for tablet hardness. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is the tableting hardness of different lotus seed treatment groups;

[0027] Figure 2 This is the curcumin release rate of curcumin tablets, Cur-LSP, and CL treatment groups at different digestion stages. DETAILED DESCRIPTION

[0028] The specific embodiments of the present invention are further described below. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in each embodiment of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0029] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are commercially available unless otherwise specified.

[0030] Embodiment 1:

[0031] This embodiment provides a method for preparing an enzymatic lotus seed tablet that promotes the digestion and release of curcumin, comprising the following steps:

[0032] (1) The preparation method of the enzyme-treated lotus seed paste is as follows: lotus seed powder (30 g) is dispersed in distilled water to prepare a 25% (w / v) powder dispersion, and the mixture is subjected to high-speed shear stirring at 12,000 rpm for 2 min. After uniform dispersion, a thermostable α-amylase (activity of 40,000 U / g, purchased from Shanghai Yuanye Biotechnology Co., Ltd.) is added to the sample at a ratio of 0.5% (based on the dry weight of the powder), and then the sample is shaken in a water bath at 55° C. for 6 h. After the reaction is completed, 5 mL of anhydrous ethanol is quickly added to the sample to terminate the enzyme reaction, thereby obtaining an enzymatically modified lotus seed paste (lotus seed slurry).

[0033] (2) Weigh 0.06 g of curcumin powder, add anhydrous ethanol, mix well, and prepare a 0.3% (w / v) curcumin ethanol solution. Then pour it into the lotus seed slurry in step (1) at a volume ratio of lotus seed slurry: curcumin ethanol solution = 4:1, stir and mix at a fixed speed with a glass rod to form a stable mixed solution. The solution does not stratify after standing for 10 hours, indicating that the prepared solution system has good stability. Then, the prepared mixed slurry is placed at -20°C for pre-freezing for 12 hours, and then freeze-dried at -80°C for 72 hours to obtain a freeze-dried sample, and the freeze-dried material is ground into powder, and sieved with a 40-mesh screen to obtain a lotus seed-curcumin composite powder. The prepared lotus seed-curcumin composite powder is vacuum-sealed (to prevent the powder from absorbing moisture), and then stored in a frozen state at -20°C (to avoid decomposition of curcumin at room temperature).

[0034] (3) Weigh 0.4 g of the composite powder prepared in step (2) and use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm) to apply a pressure of 3 tons for 3 seconds to obtain tablets CL-0.5E.

[0035] Embodiment 2:

[0036] This embodiment provides a method for preparing an enzymatically hydrolyzed lotus seed tablet that promotes the digestion and release of curcumin, comprising the following steps:

[0037] (1) The method for preparing the enzyme-treated lotus seed paste is as follows: lotus seed powder (30 g) is dispersed in distilled water to prepare a 25% (w / v) powder dispersion, and the mixture is subjected to high-speed shear stirring at 12,000 rpm for 2 min. After uniform dispersion, a thermostable α-amylase (activity of 40,000 U / g) is added to the sample at a ratio of 1.0% (based on the dry weight of the powder), and then the sample is shaken in a water bath at 55° C. for 6 h. After the reaction is completed, 5 mL of anhydrous ethanol is quickly added to the sample to terminate the enzyme reaction, thereby obtaining an enzyme-modified lotus seed paste (lotus seed slurry).

[0038] (2) Weigh 0.06 g of curcumin powder, add anhydrous ethanol, mix well, and prepare a 0.3% (w / v) curcumin ethanol solution, then pour it into the lotus seed slurry of step (1), stir and mix at a fixed speed with a glass rod to form a stable mixed solution, and the solution does not stratify after standing for 10 hours, indicating that the prepared solution system has good stability. After that, the prepared mixed slurry is placed at -20°C for pre-freezing for 12 hours, and then freeze-dried at -80°C for 72 hours to obtain a freeze-dried sample, and the freeze-dried material is ground into powder, and sieved with a 40-mesh screen to obtain a lotus seed-curcumin composite powder. The prepared lotus seed-curcumin composite powder is vacuum-sealed (to prevent the powder from absorbing moisture), and then stored in a -20°C freezer (to avoid decomposition of curcumin at room temperature).

[0039] (3) Weigh 0.4 g of the composite powder prepared in step (2) and use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm) to apply a pressure of 3 tons for 3 seconds to obtain tablets CL-1.0E.

[0040] Embodiment 3:

[0041] This embodiment provides a method for preparing an enzymatic lotus seed tablet that promotes the digestion and release of curcumin, comprising the following steps:

[0042] (1) The preparation method of the enzyme-treated lotus seed paste is as follows: lotus seed powder (30 g) is dispersed in distilled water to prepare a 25% (w / v) powder dispersion, and the mixture is subjected to high-speed shear stirring at 12,000 rpm for 2 min. After uniform dispersion, a thermostable α-amylase (activity of 40,000 U / g) is added to the sample at a ratio of 1.5% (based on the dry weight of the powder), and then the sample is shaken in a water bath at 55° C. for 6 h. After the reaction is completed, 5 mL of anhydrous ethanol is quickly added to the sample to terminate the enzyme reaction, thereby obtaining an enzymatically modified lotus seed paste (lotus seed slurry).

[0043] (2) Weigh 0.06 g of curcumin powder, add anhydrous ethanol, mix well, and prepare a 0.3% (w / v) curcumin ethanol solution. Then pour it into the lotus seed slurry of step (1), stir and mix at a fixed speed with a glass rod to form a stable mixed solution. The solution does not stratify after standing for 10 hours, indicating that the prepared solution system has good stability. After that, the prepared mixed slurry is placed at -20°C for pre-freezing for 12 hours, and then freeze-dried at -80°C for 72 hours to obtain a freeze-dried sample, and the freeze-dried material is ground into powder, and sieved with a 40-mesh screen to obtain a lotus seed-curcumin composite powder. The prepared lotus seed-curcumin composite powder is vacuum-sealed (to prevent the powder from absorbing moisture), and then stored in a -20°C freezer (to avoid decomposition of curcumin at room temperature).

[0044] (3) Weigh 0.4 g of the composite powder prepared in step (2) and use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm) to apply a pressure of 3 tons for 3 seconds to obtain tablets CL-1.5E.

[0045] Embodiment 4:

[0046] This embodiment provides a method for preparing an enzymatic lotus seed tablet that promotes the digestion and release of curcumin, comprising the following steps:

[0047] (1) The preparation method of the enzyme-treated lotus seed paste is as follows: lotus seed powder (30 g) is dispersed in distilled water to prepare a 25% (w / v) powder dispersion, and the mixture is subjected to high-speed shear stirring at 12,000 rpm for 2 min. After uniform dispersion, a thermostable α-amylase (activity of 40,000 U / g) is added to the sample at a ratio of 2.0% (based on the dry weight of the powder), and then the sample is shaken in a water bath at 55° C. for 6 h. After the reaction is completed, 5 mL of anhydrous ethanol is quickly added to the sample to terminate the enzyme reaction, thereby obtaining an enzyme-modified lotus seed paste (lotus seed slurry mixture).

[0048] (2) Weigh 0.06 g of curcumin powder, add anhydrous ethanol, mix well, and prepare a 0.3% (w / v) curcumin ethanol solution, then pour it into the lotus seed slurry of step (1), stir and mix at a fixed speed with a glass rod to form a stable mixed solution, and the solution does not stratify after standing for 10 hours, indicating that the prepared solution system has good stability. After that, the prepared mixed slurry is placed at -20°C for pre-freezing for 12 hours, and then freeze-dried at -80°C for 72 hours to obtain a freeze-dried sample, and the freeze-dried material is ground into powder, and sieved with a 40-mesh screen to obtain a lotus seed-curcumin composite powder. The prepared lotus seed-curcumin composite powder is vacuum-sealed (to prevent the powder from absorbing moisture), and then stored in a -20°C freezer (to avoid decomposition of curcumin at room temperature).

[0049] (3) Weigh 0.4 g of the composite powder prepared in step (2) and use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm) to apply a pressure of 3 tons for 3 seconds to obtain tablets CL-2.0E.

[0050] Embodiment 5:

[0051] This embodiment provides a method for preparing an enzymatic lotus seed tablet that promotes the digestion and release of curcumin, comprising the following steps:

[0052] (1) The preparation method of the enzyme-treated lotus seed paste is as follows: lotus seed powder (30 g) is dispersed in distilled water to prepare a 25% (w / v) powder dispersion, and the mixture is subjected to high-speed shear stirring at 12,000 rpm for 2 min. After uniform dispersion, a thermostable α-amylase (activity of 40,000 U / g) is added to the sample at a ratio of 2.5% (based on the dry weight of the powder), and then the sample is shaken in a water bath at 55° C. for 6 h. After the reaction is completed, 5 mL of anhydrous ethanol is quickly added to the sample to terminate the enzyme reaction, thereby obtaining an enzyme-modified lotus seed paste (lotus seed slurry).

[0053] (2) Weigh 0.06 g of curcumin powder, add anhydrous ethanol, mix well, and prepare a 0.3% (w / v) curcumin ethanol solution, then pour it into the lotus seed slurry of step (1), stir and mix at a fixed speed with a glass rod to form a stable mixed solution, and the solution does not stratify after standing for 10 hours, indicating that the prepared solution system has good stability. After that, the prepared mixed slurry is placed at -20°C for pre-freezing for 12 hours, and then freeze-dried at -80°C for 72 hours to obtain a freeze-dried sample, and the freeze-dried material is ground into powder, and sieved with a 40-mesh screen to obtain a lotus seed-curcumin composite powder. The prepared lotus seed-curcumin composite powder is vacuum-sealed (to prevent the powder from absorbing moisture), and then stored in a -20°C freezer (to avoid decomposition of curcumin at room temperature).

[0054] (3) Weigh 0.4 g of the composite powder prepared in step (2) and use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm) to apply a pressure of 3 tons for 3 seconds to obtain tablets CL-2.5E.

[0055] Comparative Example 1:

[0056] The difference between this comparative example and Example 1 is that curcumin powder is directly used for tableting without the powder preparation process. The specific operation is as follows:

[0057] Weigh 0.4 g of curcumin powder, use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm), apply a pressure of 3 tons and press for 3 seconds to obtain tablets Cur.

[0058] Comparative Example 2:

[0059] The difference between this comparative example and Example 1 is that no enzyme is added, and no enzymatic hydrolysis changes the structure of lotus seed powder. The specific operation is as follows:

[0060] (1) Lotus seed powder (30 g) was dispersed in distilled water to prepare a 25% (w / v) powder dispersion, and the mixture was subjected to high-speed shear stirring at 12,000 rpm for 2 min. After uniform dispersion, the mixture was directly incubated in an oscillation water bath at 55° C. for 6 h without adding a thermostable α-amylase, and the reaction was completed to obtain a lotus seed slurry.

[0061] (2) Weigh 0.06 g of curcumin powder, add anhydrous ethanol, mix well, and prepare a 0.3% curcumin ethanol solution. Then pour it into the lotus seed slurry of step (1), stir and mix at a fixed speed with a glass rod to form a stable mixed solution, and the solution does not stratify after standing for 10 hours, indicating that the prepared solution system has good stability. Then, the prepared mixed slurry is placed at -20°C for pre-freezing for 12 hours, and then freeze-dried at -80°C for 72 hours to obtain a freeze-dried sample, and the freeze-dried material is ground into powder, and sieved with a 40-mesh screen to obtain a lotus seed-curcumin composite powder.

[0062] (3) Weigh 0.4 g of the composite powder prepared in step (1) and use a PC-12S 12-ton tablet press and a 10 mm flat punch die (specifications: tablet size 10 mm, cavity depth 30 mm) to apply a pressure of 3 tons for 3 seconds to obtain tablets Cur-LSP.

[0063] Experimental example:

[0064] The tablets of Examples 1-5 and Comparative Examples 1-2 were subjected to a hardness test (the hardness tester used was YD-Ⅱ, manufactured by Tianjin Jingtuo Instrument Technology Co., Ltd.) to characterize the compressibility of the powder; at the same time, the tablets were subjected to in vitro simulated gastric and intestinal digestion to determine the cumulative release rate of curcumin at different digestion stages.

[0065] Among them, the in vitro digestion method of curcumin-lotus seed-based tablets is as follows:

[0066] In order to evaluate the effect of lotus seed powder and curcumin complex formation in different enzyme treatment groups, the in vitro simulated digestion was performed according to the method of Yu et al. (Yu Y-B, Wu M-Y, Wang C, Wang ZW, Chen TT and Yan J-K, Constructing biocompatible carboxylic curdlan-coated zein nanoparticles for curcumin encapsulation. Food Hydrocoll 108: 106028 (2020).) with slight modifications. Due to the short residence time of the substance in the oral cavity, the oral digestion of the tablet was not considered in this study (Minekus M, Alminger M, Alvito P, Ballance S, Bohn T, Bourlieu C et al., A standardised static in vitro digestion method suitable for food: an international consensus. Food Funct 5: 1113-1124 (2014).).

[0067] 20 mL of freshly prepared simulated gastric fluid (SGF, pH 1.5, containing 10.0 mg / mL pepsin) was mixed with an equal volume of anhydrous ethanol, and then 0.4 g of lotus seed powder-curcumin tablets were added. After thorough mixing, the mixture was placed in a constant temperature water bath oscillator at 37°C, and the reaction was carried out at 120 rpm in the dark for 2 hours. After the reaction, the pH was adjusted to 7.0 with 1 mol / L NaOH to terminate the gastric digestion reaction. The obtained gastric digestion dispersion was mixed with an equal amount of simulated intestinal fluid (SIF; pH 7.0, containing 10.0 mg / mL pancreatin) and intestinal digestion was carried out for 4 hours in the above-mentioned constant temperature shaker (37°C, 100 rpm). Samples of the SGF and SIF digestion stages were collected every 1 hour, 1.0 mL was extracted each time, centrifuged at 2000×g for 10 minutes, the supernatant was separated and collected, and after dilution with 3 mL of anhydrous ethanol, the absorbance was measured at a wavelength of 432 nm. Finally, the cumulative release of curcumin was determined using the following formula:

[0068]

[0069] like Figure 1As shown in the figure, LSP is directly compressed with lotus seed powder, that is, the original lotus seed powder is directly compressed (0.4 grams of lotus seed powder is weighed and 3 tons of pressure is applied for 3 seconds). Compared with LSP (52.90±0.10N), the tablet hardness of Cur-LSP is greatly improved, reaching 387.50±36.96N, indicating that the water bath heating process can significantly improve the compressibility of lotus seed powder, which may be due to the gelatinization and viscosity increase of lotus seed starch during the water bath heating process. After adding an appropriate amount of amylase (ie, CL group), the hardness of the tablet is slightly reduced, indicating that the tablet structure formed by the enzymatic hydrolysis group is slightly looser than that of the blank group (Cur-LSP). In the CL treatment group, when the enzyme addition amount is 1.5%, the tablet hardness is the highest (319.60±33.36N), that is, the compressibility of lotus seed powder is improved the most.

[0070] The above experimental results show that the hardness of the enzymatic tablets is appropriate (not too high or too low). Appropriate hardness is conducive to the disintegration, digestion and absorption of the tablets (enzymatic hydrolysis promotes the decomposition of starch into small molecule sugars, which is beneficial to the human body's utilization of nutrients). Hardness can reflect the firmness of the tablet structure. Theoretically, at an enzyme addition of 1.5%, the degree of enzymatic hydrolysis is the greatest, the tablet structure is the loosest, and the hardness value should be the lowest, but the actual hardness value is the highest. This shows that the firmness of lotus seed tablets may be affected by intermolecular forces in addition to the degree of enzymatic hydrolysis. The chemical bonds formed by the CL-1.5E group strengthen the tablet structure, so the tablet structure formed is more firm and has a higher hardness value.

[0071] The release rate of curcumin was measured for Examples 1-5 and Comparative Examples 1-2. The results are as follows: Figure 2 As shown, the release degree of comparative example 1 is almost zero (1.78 ± 0.03%), and the release degree of curcumin is extremely low, indicating that when the proportion of curcumin in the tablet matrix is ​​too high, it is not conducive to its release. Therefore, the appropriate active factor concentration (the proportion of curcumin) is crucial to the release rate. The release degree of comparative example 2 is low (16.64 ± 1.06%), and curcumin is also difficult to release from the tablet matrix. After adding heat-stable α-amylase, the release rate increases significantly. When the enzyme addition amount is 1.5%, the release rate of curcumin reaches 40.35 ± 0.33%. Therefore, the optimal enzyme addition amount in this experiment is 1.5%. The above results show that the addition of α-amylase changes the structure of lotus seed powder, affects the combination mode with curcumin, and while greatly improving the hardness, the tablet structure formed has good disintegration performance (which is conducive to the dissolution of curcumin).

[0072] In addition, in terms of sensory properties, enzymatic tablets have a distinct aroma. Due to the action of a-amylase, starch is decomposed to form reducing sugars, which react with proteins to produce complex flavor substances through Maillard reaction. Enzymatic hydrolysis is an effective way to give products flavor. In summary, CL-1.5E performs well in tablet hardness, release rate, sensory flavor, etc., and is a high-quality nutritional health tablet.

[0073] The embodiments of the present invention are described in detail above, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the protection scope of the present invention.

Claims

1. A curcumin lotus seed tablet, characterized in that: The raw materials of the curcumin lotus seed tablets include curcumin, lotus seed powder and α-amylase. The mass ratio of the curcumin to the lotus seed powder is 1:400-600, and the dosage of the α-amylase is 0.5%-2.5% of the mass of the lotus seed powder.

2. The method for preparing the curcumin lotus seed tablet according to claim 1, characterized in that: The following steps are involved: S1, dispersing lotus seed powder in water, stirring at high speed, adding α-amylase, and obtaining lotus seed slurry after enzymolysis; S2, dissolving curcumin in an organic solvent to prepare a curcumin solution, and then mixing the curcumin solution with the lotus seed slurry of step S1, and drying the obtained mixed slurry to obtain a curcumin-lotus seed composite powder, and then tableting the composite powder to obtain a curcumin lotus seed tablet.

3. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: S1 The α-amylase is a thermostable α-amylase, which is prepared by fermentation, extraction and purification of Bacillus licheniformis. The activity of the thermostable α-amylase is 40,000 U / g.

4. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: The enzymolysis in S1 is water bath enzymolysis at a temperature of 50-60° C. for 5-7 h.

5. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: S1: After the high-speed shear stirring, high-speed shear stirring is performed at a speed of 11000-15000 rpm for 1-5 minutes.

6. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: S1: dispersing lotus seed powder in water to prepare a powder dispersion with a mass concentration of 20-30%.

7. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: In S2, after the curcumin-lotus seed composite powder is prepared, it is vacuum-sealed and stored in a frozen state at -20°C.

8. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: S2 dissolves curcumin in an organic solvent to prepare a curcumin solution with a mass concentration of 0.2-0.5%.

9. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: The drying in S2 is to pre-freeze at -20°C for 10-15h and then freeze-dry at -80°C for 70-80h to prepare freeze-dried powder.

10. The method for preparing the curcumin lotus seed tablet according to claim 2, characterized in that: S2 The organic solvent comprises ethanol.

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