Astaxanthin mask as well as preparation method and application thereof

Abstract

The invention discloses an astaxanthin mask as well as a preparation method and application thereof, and belongs to the technical field of skin care product processing. The astaxanthin mask is prepared from astaxanthin, a gynura succulent extract, an eclipta prostrata extract, an artemisia stelleriana extract, a trifolium repens extract, an artemisia scoparia extract, a chondrus crispus extract, an Okouguman tree resin extract, an aloe extract and conventional auxiliary materials during skin care product preparation. The components in the mask cooperate with each other and are easily absorbed by human skin. More possibilities are provided for practical application of astaxanthin, meanwhile, the astaxanthin mask has good whitening, moisturizing and anti-oxidation effects, the skin can keep good elasticity and compactness, skin discomfort can be effectively relieved, and the good acne removing function is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of skin care product processing, and in particular to an astaxanthin facial mask, a preparation method and application thereof. Background Art

[0002] Facial mask is a common skin care product in daily life. It uses the short time of covering the face to promote the expansion of skin pores, promote sweat gland secretion and metabolism, increase the skin's oxygen content, so as to promote the skin to excrete the products of surface cell metabolism and accumulated oil substances, and allow the high concentration of nutrients in the mask liquid to quickly enter the skin, thereby achieving fast and effective skin care.

[0003] Astaxanthin, also known as astaxanthin, is a keto-carotenoid that is widely found in the biological world. It has strong effects in inhibiting tumor occurrence and growth, anti-oxidation, resisting ultraviolet rays, enhancing human immune function, improving vision, pigment formation and nerve connectivity, and improving fertility. In particular, its antioxidant capacity is super strong, which is 6,000 times that of vitamin C and 1,000 times that of vitamin E. It is known as a "super antioxidant". Studies have found that many types of algae contain astaxanthin, among which the astaxanthin produced by Haematococcus pluvialis has the characteristics of fast cell reproduction, simple cultivation, and easy extraction. Astaxanthin can be extracted from Haematococcus pluvialis by supercritical CO2 and nano-emulsified to ensure activity. The obtained astaxanthin can be directly absorbed by the skin and has the characteristics of high water solubility, high activity, and high purity.

[0004] As people's living standards improve, they pay more and more attention to skin care and have higher and higher requirements for skin care products. At the same time, many factors such as bad living habits, harsh environment, ultraviolet rays, various mental pressures, improper skin care, etc. cause great harm to the skin. More and more people are troubled by comprehensive problems such as dull skin, roughness, and lack of elasticity. Although there are many skin care products on the market, they can not always solve this problem well. Often, solving one problem will bring about other problems at the same time. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the present invention proposes an astaxanthin facial mask, a preparation method and application thereof to solve the problems raised by the above-mentioned background technology.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0008] In terms of weight, the components of group A include the following raw materials: 12 to 20 parts of astaxanthin, 2 to 5 parts of bifida yeast extract, 1 to 10 parts of lactic acid bacteria fermentation extract, 432 to 720 parts of fine algae polysaccharide, and 1200 to 2000 parts of trehalose;

[0009] In terms of weight, the B group includes the following raw materials: 1.20-2.00 parts of Dilsea carnosa extract, 0.48-0.80 parts of Eclipta prostrata extract, 0.48-0.80 parts of Artemisia abrotanum extract, 0.24-0.40 parts of Trifolium repens extract, 0.24-0.40 parts of Artemisia scoparia extract, 2.40-4.00 parts of Chondrus Crispus extract, 0.12-0.20 parts of Aucoume aclaineana resin extract, and 0.12-0.20 parts of aloe extract;

[0010] In terms of weight, the C group includes the following raw materials: 16800-28000 parts of glycerin, 108-180 parts of hydrolyzed sclerotium gum, 0.000744-0.001240 parts of valine, 0.48-0.80 parts of collagen, 240-400 parts of hyaluronic acid mixture, 720-1200 parts of niacinamide, and 206400-344000 parts of water;

[0011] In terms of weight, the D component includes the following raw materials: 1440 to 2400 parts of betaine, 168 to 280 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 239 to 398 parts of hydroxyethylpiperazine ethane sulfonic acid, 1 to 6 parts of carbomer, 240 to 400 parts of allantoin, 0.0024 to 0.0040 parts of p-hydroxyacetophenone, and 0.30 to 1.50 parts of bosine.

[0012] Preferably, the hyaluronic acid mixture is formed by uniformly mixing 0.84 to 1.40 parts by weight of sodium hyaluronate cross-linked polymer, 1.80 to 3.00 parts by weight of sodium hyaluronate, 2.10 to 3.50 parts by weight of acetylated sodium hyaluronate, and 0.36 to 0.60 parts by weight of hydrolyzed sodium hyaluronate.

[0013] Preferably, the method for preparing the succulent red vegetable extract comprises the following steps:

[0014] (1) Washing the red succulent vegetable, drying and crushing it, adding the crushed red succulent vegetable to a 5-10% by mass sodium bicarbonate aqueous solution to obtain a mixture, gradually heating the mixture to 52-58° C., stirring at a speed of 45-55 rpm for 7-10 hours, and cooling the mixture to 25-28° C. after the stirring is completed;

[0015] (2) The mixture is first beaten to form a slurry, and then the pH value of the slurry is adjusted to 6.8-7.8 with an acid solution, and then a composite enzyme is added for enzymolysis for 60-100 minutes at a temperature of 35-42° C. After the enzymolysis is completed, the solid-liquid separation is performed to obtain an enzymolysis solution, which is then concentrated at low temperature and reduced pressure to 60-70% of the original volume to obtain a succulent red vegetable extract.

[0016] Preferably, the succulent red vegetable and the sodium bicarbonate aqueous solution are mixed at a solid-liquid ratio of 1:(15-20), and the added amount of the complex enzyme is 0.5-3% of the total mass of the slurry.

[0017] Preferably, the complex enzyme is a mixture of β-glucanase, β-xylosidase and cellulase in a mass ratio of 1:(1-2.5):(3-7).

[0018] Preferably, the β-glucanase activity is 20,000 U / g, the β-xylosidase activity is 1,000 U / g, and the cellulase activity is 100,000 U / g.

[0019] Preferably, the preparation method of the snakehead intestine extract comprises the following steps: crushing the snakehead intestine, adding an ethanol aqueous solution with a mass concentration of 70-80%, with a solid-liquid ratio of 1:(5-10), soaking for 80-150 minutes, refluxing extraction at a temperature of 40-50°C for 40-60 minutes, refluxing extraction 2-4 times, filtering, combining the filtrate, concentrating under reduced pressure, and freeze-drying to obtain the snakehead intestine extract.

[0020] Preferably, the freeze-drying temperature is -45 to -35°C.

[0021] Preferably, the method for preparing the Artemisia australis extract comprises the following steps:

[0022] (1) mixing chopped whole Artemisia australis herb and anhydrous ethanol in a mass ratio of 1:(8-12), stirring at a temperature of 50-55° C. for 30-60 minutes to obtain an initial mixture;

[0023] (2) filtering the initial mixture for the first time to obtain a first filtrate;

[0024] (3) refrigerating the first filtrate at a temperature of 1 to 4° C. for 3 to 7 hours to obtain a refrigerated liquid;

[0025] (4) filtering the refrigerated liquid for a second time to obtain a second filtrate and solid residue;

[0026] (5) placing the second filtrate under vacuum conditions of 0.04-0.08 MPa and a temperature of 60-65° C. for evaporation concentration for 25-35 min to obtain a concentrate;

[0027] (6) mixing the solid residue and the concentrate and drying them to obtain an Artemisia argyi extract.

[0028] Preferably, the method for preparing the astaxanthin facial mask comprises the following steps:

[0029] S1. Add the C group components into the emulsifying pot, stir and heat to 70-80℃ to dissolve evenly;

[0030] S2. Add the components of group D into the emulsifying pot after stirring and dispersing evenly, and cool to 30-40°C;

[0031] S3, stirring and dissolving the components of group A and group B respectively and adding them into the emulsifying pot to obtain a facial mask liquid;

[0032] S4. Soak the mask matrix in the mask liquid to obtain the astaxanthin mask.

[0033] Preferably, the astaxanthin facial mask is used in the preparation of skin care products that maintain skin firmness and wrinkle resistance, moisturize and hydrate, whiten, resist oxidation, remove acne and soothe the skin.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] (1) The astaxanthin facial mask of the present invention comprises astaxanthin, red vegetable extract, Channa intestine extract, Artemisia australis extract, Trifolium repens extract, Artemisia spatholobi extract, Chondrus crispus extract, Okoume tree resin extract, Aloe vera extract and conventional auxiliary materials for preparing skin care products. The plant extracts can soothe sensitive skin and repair damaged skin cells. The ingredients in the facial mask cooperate with each other and are easily absorbed by human skin. At the same time, the present invention provides more possibilities for the practical application of astaxanthin. At the same time, the astaxanthin facial mask has good whitening, moisturizing and anti-oxidation effects, can keep the skin in good elasticity and firmness, effectively soothe skin discomfort, and has good acne removal function.

[0036] (2) The extract of red cabbage is extracted by alkaline hydrolysis + enzymatic hydrolysis, and the red cabbage is processed in sections, which not only reduces the effect of extraction conditions on the activity of the final extract, but also has a high extraction efficiency. First, a weakly alkaline sodium bicarbonate aqueous solution is used as an alkaline hydrolysis solution. On the one hand, it reduces the pH of the solution and eases the extraction conditions. On the other hand, alkaline hydrolysis destroys the cell walls of the red cabbage cells, improves the permeability of the red cabbage cells, and is conducive to improving the subsequent enzymatic hydrolysis efficiency; then, the enzymatic hydrolysis treatment is carried out by a composite enzyme, which can fully enzymatically hydrolyze the cell walls, binding proteins and saccharification products, and promote the dissolution of intracellular substances of the red cabbage. At the same time, the extraction process adopts a low-temperature process not exceeding 45°C throughout the process, which effectively retains the activity of the red cabbage extract.

[0037] (3) A hyaluronic acid mixture is formed by uniformly mixing sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate. It can not only form a protective film on the skin and reduce water loss, but also replenish skin moisture and have a moisturizing effect. DETAILED DESCRIPTION

[0038] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0039] Example 1

[0040] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0041] In terms of weight, the components of group A include the following raw materials: 12 parts of astaxanthin, 2 parts of bifida yeast extract, 1 part of lactic acid bacteria fermentation extract, 432 parts of fine algae polysaccharide, and 1200 parts of trehalose;

[0042] In terms of weight, the B group includes the following raw materials: 1.20 parts of succulent red cabbage extract, 0.48 parts of Channa intestine extract, 0.48 parts of Artemisia sutchuenensis extract, 0.24 parts of Trifolium repens extract, 0.24 parts of Artemisia scoparia extract, 2.40 parts of Chondrus crispus extract, 0.12 parts of Okoume resin extract, and 0.12 parts of Aloe vera extract;

[0043] In terms of weight, the C group ingredients include the following raw materials: 16,800 parts of glycerin, 108 parts of hydrolyzed sclerotium gum, 0.000744 parts of valine, 0.48 parts of collagen, 240 parts of hyaluronic acid mixture, 720 parts of niacinamide, and 206,400 parts of water;

[0044] In terms of weight, the D component includes the following raw materials: 1440 parts of betaine, 168 parts of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 239 parts of hydroxyethylpiperazine ethane sulfonic acid, 1 part of carbomer, 240 parts of allantoin, 0.0024 parts of p-hydroxyacetophenone, and 0.30 parts of bosine.

[0045] The hyaluronic acid mixture is prepared by uniformly mixing 0.84 parts by weight of sodium hyaluronate cross-linked polymer, 1.80 parts by weight of sodium hyaluronate, 2.10 parts by weight of acetylated sodium hyaluronate, and 0.36 parts by weight of hydrolyzed sodium hyaluronate.

[0046] The preparation method of the succulent red vegetable extract comprises the following steps:

[0047] (1) Wash the red succulent vegetable, dry and crush it, add the crushed red succulent vegetable into a 5% sodium bicarbonate aqueous solution, and mix them at a material-liquid ratio of 1:15 to obtain a mixture, gradually heat the mixture to 52° C., stir at a speed of 45 rpm for 7 hours, and cool the mixture to 25° C. after stirring;

[0048] (2) The mixture is first beaten to form a slurry, and then the pH value of the slurry is adjusted to 6.8 with an acid solution, and then a composite enzyme accounting for 0.5% of the total mass of the slurry is added for enzymatic hydrolysis for 60 minutes at a temperature of 35° C. After the enzymatic hydrolysis is completed, the solid-liquid separation is performed to obtain an enzymatic hydrolyzate, which is then concentrated at low temperature and reduced pressure to 60% of the original volume to obtain a succulent red vegetable extract.

[0049] The complex enzyme is prepared by mixing beta-glucanase, beta-xylosidase and cellulase in a mass ratio of 1:1:3.

[0050] The preparation method of the snakehead intestine extract comprises the following steps: crushing the snakehead intestine, adding an ethanol aqueous solution with a mass concentration of 70% at a solid-liquid ratio of 1:5, soaking for 80 minutes, refluxing and extracting at 40°C for 40 minutes, refluxing and extracting twice, filtering, combining the filtrate, concentrating under reduced pressure, and freeze-drying at -45°C to obtain the snakehead intestine extract.

[0051] The preparation method of the Artemisia argyi extract comprises the following steps:

[0052] (1) mixing chopped Artemisia australis whole herb and anhydrous ethanol in a mass ratio of 1:8, and stirring at a temperature of 50° C. for 30 minutes to obtain an initial mixture;

[0053] (2) filtering the initial mixture for the first time to obtain a first filtrate;

[0054] (3) refrigerating the first filtrate at 1° C. for 3 h to obtain a refrigerated solution;

[0055] (4) filtering the refrigerated liquid for a second time to obtain a second filtrate and solid residue;

[0056] (5) placing the second filtrate under vacuum conditions of 0.04 MPa and 60° C. for evaporation concentration for 25 min to obtain a concentrate;

[0057] (6) mixing the solid residue and the concentrate and drying them to obtain an Artemisia argyi extract.

[0058] A method for preparing an astaxanthin facial mask comprises the following steps:

[0059] S1. Add the C group components into the emulsifying pot, stir and heat to 70℃ to dissolve evenly;

[0060] S2. Add the components of group D into the emulsifying pot after stirring and dispersing evenly, and cool to 30°C after stirring;

[0061] S3, stirring and dissolving the components of group A and group B respectively and adding them into the emulsifying pot to obtain a facial mask liquid;

[0062] S4. Soak the mask matrix in the mask liquid to obtain the astaxanthin mask.

[0063] Example 2

[0064] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0065] In terms of weight, the components of group A include the following raw materials: 20 parts of astaxanthin, 5 parts of bifida yeast extract, 10 parts of lactic acid bacteria fermentation extract, 720 parts of fine algae polysaccharide, and 2000 parts of trehalose;

[0066] In terms of weight, the B group includes the following raw materials: 2.00 parts of succulent red vegetable extract, 0.80 parts of Channa intestine extract, 0.80 parts of Artemisia australis extract, 0.40 parts of Trifolium repens extract, 0.40 parts of Artemisia scoparia extract, 4.00 parts of Chondrus crispus extract, 0.20 parts of Okoume resin extract, and 0.20 parts of Aloe vera extract;

[0067] In terms of weight, the C group ingredients include the following raw materials: 28,000 parts of glycerin, 180 parts of hydrolyzed sclerotium gum, 0.001240 parts of valine, 0.80 parts of collagen, 400 parts of hyaluronic acid mixture, 1,200 parts of niacinamide, and 344,000 parts of water;

[0068] In terms of weight, the D component includes the following raw materials: 2400 parts of betaine, 280 parts of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 398 parts of hydroxyethylpiperazine ethane sulfonic acid, 6 parts of carbomer, 400 parts of allantoin, 0.0040 parts of p-hydroxyacetophenone, and 1.50 parts of bosine.

[0069] The hyaluronic acid mixture is prepared by uniformly mixing 1.40 parts by weight of sodium hyaluronate cross-linked polymer, 3.00 parts by weight of sodium hyaluronate, 3.50 parts by weight of acetylated sodium hyaluronate, and 0.60 parts by weight of hydrolyzed sodium hyaluronate.

[0070] The preparation method of the succulent red vegetable extract comprises the following steps:

[0071] (1) Wash the red succulent vegetable, dry and crush it, add the crushed red succulent vegetable to a 10% sodium bicarbonate aqueous solution, mix them at a material-liquid ratio of 1:20 to obtain a mixture, gradually heat the mixture to 58° C., stir at a speed of 55 rpm for 10 hours, and cool the mixture to 28° C. after stirring;

[0072] (2) The mixture is first beaten to form a slurry, and then the pH value of the slurry is adjusted to 7.8 with an acid solution, and then a composite enzyme accounting for 3% of the total mass of the slurry is added for enzymatic hydrolysis for 100 minutes at a hydrolysis temperature of 42° C. After the enzymatic hydrolysis is completed, the solid-liquid separation is performed to obtain an enzymatic hydrolyzate, which is then concentrated at low temperature and reduced pressure to 70% of the original volume to obtain a succulent red vegetable extract.

[0073] The complex enzyme is prepared by mixing beta-glucanase, beta-xylosidase and cellulase in a mass ratio of 1:2.5:7.

[0074] The preparation method of the snakehead intestine extract comprises the following steps: crushing the snakehead intestine, adding an ethanol aqueous solution with a mass concentration of 80% at a solid-liquid ratio of 1:10, soaking for 150 minutes, reflux extraction at 50°C for 60 minutes, reflux extraction for 4 times, filtering, combining the filtrate, concentrating under reduced pressure, and freeze-drying at -35°C to obtain the snakehead intestine extract.

[0075] The preparation method of the Artemisia argyi extract comprises the following steps:

[0076] (1) mixing chopped Artemisia australis whole herb and anhydrous ethanol at a mass ratio of 1:12, and stirring at a temperature of 55° C. for 60 minutes to obtain an initial mixture;

[0077] (2) filtering the initial mixture for the first time to obtain a first filtrate;

[0078] (3) refrigerating the first filtrate at 4° C. for 7 h to obtain a refrigerated solution;

[0079] (4) filtering the refrigerated liquid for a second time to obtain a second filtrate and solid residue;

[0080] (5) placing the second filtrate under vacuum conditions of 0.08 MPa and 65° C. for evaporation concentration for 35 min to obtain a concentrate;

[0081] (6) mixing the solid residue and the concentrate and drying them to obtain an Artemisia argyi extract.

[0082] A method for preparing an astaxanthin facial mask comprises the following steps:

[0083] S1. Add the C group components into the emulsifying pot, stir and heat to 80℃ to dissolve evenly;

[0084] S2. Stir and disperse the components of group D evenly and add them into the emulsifying pot, stir and cool to 40°C;

[0085] S3, stirring and dissolving the components of group A and group B respectively and adding them into the emulsifying pot to obtain a facial mask liquid;

[0086] S4. Soak the mask matrix in the mask liquid to obtain the astaxanthin mask.

[0087] Example 3

[0088] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0089] In terms of weight, the components of group A include the following raw materials: 18 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0090] In terms of weight, the B group includes the following raw materials: 1.60 parts of succulent red vegetable extract, 0.70 parts of Channa intestine extract, 0.60 parts of Artemisia sutchuenensis extract, 0.30 parts of Trifolium repens extract, 0.35 parts of Artemisia scoparia extract, 3.00 parts of Chondrus crispus extract, 0.18 parts of Okoume tree resin extract, and 0.15 parts of Aloe vera extract;

[0091] In terms of weight, the C group ingredients include the following raw materials: 20,000 parts of glycerin, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 300 parts of hyaluronic acid mixture, 1,000 parts of niacinamide, and 300,000 parts of water;

[0092] In terms of weight, the D component includes the following raw materials: 2000 parts of betaine, 200 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 300 parts of hydroxyethylpiperazine ethane sulfonic acid, 3 parts of carbomer, 300 parts of allantoin, 0.0030 parts of p-hydroxyacetophenone, and 1.00 parts of bosine.

[0093] The hyaluronic acid mixture is prepared by uniformly mixing 1.20 parts by weight of sodium hyaluronate cross-linked polymer, 2.20 parts by weight of sodium hyaluronate, 2.50 parts by weight of acetylated sodium hyaluronate, and 0.40 parts by weight of hydrolyzed sodium hyaluronate.

[0094] The preparation method of the succulent red vegetable extract comprises the following steps:

[0095] (1) Wash the red succulent vegetable, dry and crush it, add the crushed red succulent vegetable to an aqueous sodium bicarbonate solution with a mass fraction of 8%, and mix them at a solid-liquid ratio of 1:18 to obtain a mixture, gradually heat the mixture to 55° C., stir it at a speed of 50 rpm for 9 hours, and cool it to 26° C. after the stirring is completed;

[0096] (2) The mixture is first beaten to form a slurry, and then the pH value of the slurry is adjusted to 7.0 with an acid solution, and then a composite enzyme accounting for 2% of the total mass of the slurry is added for enzymatic hydrolysis for 80 minutes at a hydrolysis temperature of 40° C. After the enzymatic hydrolysis is completed, the solid-liquid separation is performed to obtain an enzymatic hydrolyzate, which is then concentrated at low temperature and reduced pressure to 65% of the original volume to obtain a succulent red vegetable extract.

[0097] The complex enzyme is prepared by mixing beta-glucanase, beta-xylosidase and cellulase in a mass ratio of 1:2:4.

[0098] The preparation method of the snakehead intestine extract comprises the following steps: crushing the snakehead intestine, adding an ethanol aqueous solution with a mass concentration of 75% at a solid-liquid ratio of 1:8, soaking for 100 minutes, refluxing and extracting at a temperature of 45°C for 50 minutes, refluxing and extracting three times, filtering, combining the filtrate, concentrating under reduced pressure, and freeze-drying at a temperature of -40°C to obtain the snakehead intestine extract.

[0099] The preparation method of the Artemisia argyi extract comprises the following steps:

[0100] (1) mixing chopped Artemisia australis whole herb and anhydrous ethanol at a mass ratio of 1:10, and stirring at a temperature of 52° C. for 40 minutes to obtain an initial mixture;

[0101] (2) filtering the initial mixture for the first time to obtain a first filtrate;

[0102] (3) refrigerating the first filtrate at 3° C. for 5 hours to obtain a refrigerated solution;

[0103] (4) filtering the refrigerated liquid for a second time to obtain a second filtrate and solid residue;

[0104] (5) placing the second filtrate under vacuum conditions of 0.06 MPa and 62° C. for evaporation concentration for 30 min to obtain a concentrate;

[0105] (6) mixing the solid residue and the concentrate and drying them to obtain an Artemisia argyi extract.

[0106] A method for preparing an astaxanthin facial mask comprises the following steps:

[0107] S1. Add the C group ingredients into the emulsifying pot, stir and heat to 75℃ to dissolve evenly;

[0108] S2. Add the components of group D into the emulsifying pot after stirring and dispersing evenly, and cool to 35°C;

[0109] S3, stirring and dissolving the components of group A and group B respectively and adding them into the emulsifying pot to obtain a facial mask liquid;

[0110] S4. Soak the mask matrix in the mask liquid to obtain the astaxanthin mask.

[0111] Comparative Example 1

[0112] The difference between this comparative example and Example 3 is that the succulent red vegetable extract is not subjected to enzymatic hydrolysis, and the other components and steps remain unchanged.

[0113] The preparation method of the succulent red vegetable extract comprises the following steps:

[0114] (1) Wash the red succulent vegetable, dry and crush it, add the crushed red succulent vegetable to an aqueous sodium bicarbonate solution with a mass fraction of 8%, and mix them at a solid-liquid ratio of 1:18 to obtain a mixture, gradually heat the mixture to 55° C., stir it at a speed of 50 rpm for 9 hours, and cool it to 26° C. after the stirring is completed;

[0115] (2) The mixture is first beaten to form a slurry, and then the pH value of the slurry is adjusted to 7.0 with an acid solution, solid-liquid separation is performed, and the filtrate is concentrated at low temperature and reduced pressure to 65% of the original volume to obtain the succulent red vegetable extract.

[0116] Comparative Example 2

[0117] The difference between this comparative example and Example 3 is that the Artemisia annua extract in Group B is replaced with Artemisia annua extract, and the other ingredients and steps remain unchanged.

[0118] In terms of weight, the B group includes the following raw materials: 1.60 parts of succulent red vegetable extract, 0.70 parts of Channa intestine extract, 0.60 parts of Artemisia annua extract, 0.30 parts of Trifolium repens extract, 0.35 parts of Artemisia scoparia extract, 3.00 parts of Chondrus crispus extract, 0.18 parts of Okoume tree resin extract, and 0.15 parts of Aloe vera extract;

[0119] The preparation method of the Artemisia annua extract comprises the following steps:

[0120] (1) chopped whole Artemisia annua herb and anhydrous ethanol were mixed in a mass ratio of 1:10, and stirred at 52° C. for 40 min to obtain an initial mixture;

[0121] (2) filtering the initial mixture for the first time to obtain a first filtrate;

[0122] (3) refrigerating the first filtrate at 3° C. for 5 hours to obtain a refrigerated solution;

[0123] (4) filtering the refrigerated liquid for a second time to obtain a second filtrate and solid residue;

[0124] (5) placing the second filtrate under vacuum conditions of 0.06 MPa and 62° C. for evaporation concentration for 30 min to obtain a concentrate;

[0125] (6) mixing the solid residue and the concentrate and drying them to obtain an Artemisia annua extract.

[0126] Comparative Example 3

[0127] The difference between this comparative example and Example 3 is that no succulent red vegetable extract is added to Group B, and the other ingredients and steps remain unchanged.

[0128] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0129] In terms of weight, the components of group A include the following raw materials: 18 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0130] In terms of weight, the B group includes the following raw materials: 0.70 parts of Channa intestine extract, 0.60 parts of Artemisia sutchuenensis extract, 0.30 parts of Trifolium repens extract, 0.35 parts of Artemisia scoparia extract, 3.00 parts of Chondrus crispus extract, 0.18 parts of Okoume resin extract, and 0.15 parts of Aloe vera extract;

[0131] In terms of weight, the C group ingredients include the following raw materials: 20,000 parts of glycerin, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 300 parts of hyaluronic acid mixture, 1,000 parts of niacinamide, and 300,000 parts of water;

[0132] In terms of weight, the D component includes the following raw materials: 2000 parts of betaine, 200 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 300 parts of hydroxyethylpiperazine ethane sulfonic acid, 3 parts of carbomer, 300 parts of allantoin, 0.0030 parts of p-hydroxyacetophenone, and 1.00 parts of bosine.

[0133] The hyaluronic acid mixture is prepared by uniformly mixing 1.20 parts by weight of sodium hyaluronate cross-linked polymer, 2.20 parts by weight of sodium hyaluronate, 2.50 parts by weight of acetylated sodium hyaluronate, and 0.40 parts by weight of hydrolyzed sodium hyaluronate.

[0134] Comparative Example 4

[0135] The difference between this comparative example and Example 3 is that no Channa intestine extract is added to Group B, and the other ingredients and steps remain unchanged.

[0136] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0137] In terms of weight, the components of group A include the following raw materials: 18 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0138] In terms of weight, the B group includes the following raw materials: 1.60 parts of succulent red cabbage extract, 0.60 parts of southern artemisia extract, 0.30 parts of white clover extract, 0.35 parts of artemisia scoparia extract, 3.00 parts of crisp wave chondrus extract, 0.18 parts of okoume resin extract, and 0.15 parts of aloe extract;

[0139] In terms of weight, the C group ingredients include the following raw materials: 20,000 parts of glycerin, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 300 parts of hyaluronic acid mixture, 1,000 parts of niacinamide, and 300,000 parts of water;

[0140] In terms of weight, the D component includes the following raw materials: 2000 parts of betaine, 200 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 300 parts of hydroxyethylpiperazine ethane sulfonic acid, 3 parts of carbomer, 300 parts of allantoin, 0.0030 parts of p-hydroxyacetophenone, and 1.00 parts of bosine.

[0141] The hyaluronic acid mixture is prepared by uniformly mixing 1.20 parts by weight of sodium hyaluronate cross-linked polymer, 2.20 parts by weight of sodium hyaluronate, 2.50 parts by weight of acetylated sodium hyaluronate, and 0.40 parts by weight of hydrolyzed sodium hyaluronate.

[0142] Comparative Example 5

[0143] The difference between this comparative example and Example 3 is that no Artemisia argyi extract is added to Group B, and the other ingredients and steps remain unchanged.

[0144] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0145] In terms of weight, the components of group A include the following raw materials: 18 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0146] In terms of weight, the B group includes the following raw materials: 1.60 parts of succulent red vegetable extract, 0.70 parts of Channa intestine extract, 0.30 parts of white clover extract, 0.35 parts of Artemisia scoparia extract, 3.00 parts of crisp wave chondrus extract, 0.18 parts of okoume tree resin extract, and 0.15 parts of aloe extract;

[0147] In terms of weight, the C group ingredients include the following raw materials: 20,000 parts of glycerin, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 300 parts of hyaluronic acid mixture, 1,000 parts of niacinamide, and 300,000 parts of water;

[0148] In terms of weight, the D component includes the following raw materials: 2000 parts of betaine, 200 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 300 parts of hydroxyethylpiperazine ethane sulfonic acid, 3 parts of carbomer, 300 parts of allantoin, 0.0030 parts of p-hydroxyacetophenone, and 1.00 parts of bosine.

[0149] The hyaluronic acid mixture is prepared by uniformly mixing 1.20 parts by weight of sodium hyaluronate cross-linked polymer, 2.20 parts by weight of sodium hyaluronate, 2.50 parts by weight of acetylated sodium hyaluronate, and 0.40 parts by weight of hydrolyzed sodium hyaluronate.

[0150] Comparative Example 6

[0151] The difference between this comparative example and Example 3 is that: the extract of Trifolium repens and the extract of Artemisia scoparia are not added to the components of Group B, and the other components and steps remain unchanged.

[0152] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0153] In terms of weight, the components of group A include the following raw materials: 18 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0154] In terms of weight, the B group includes the following raw materials: 1.60 parts of succulent red vegetable extract, 0.70 parts of Channa intestine extract, 0.60 parts of Artemisia sutchuenensis extract, 3.00 parts of Chondrus crispus extract, 0.18 parts of Okoume tree resin extract, and 0.15 parts of Aloe vera extract;

[0155] In terms of weight, the C group ingredients include the following raw materials: 20,000 parts of glycerin, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 300 parts of hyaluronic acid mixture, 1,000 parts of niacinamide, and 300,000 parts of water;

[0156] In terms of weight, the D component includes the following raw materials: 2000 parts of betaine, 200 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 300 parts of hydroxyethylpiperazine ethane sulfonic acid, 3 parts of carbomer, 300 parts of allantoin, 0.0030 parts of p-hydroxyacetophenone, and 1.00 parts of bosine.

[0157] The hyaluronic acid mixture is prepared by uniformly mixing 1.20 parts by weight of sodium hyaluronate cross-linked polymer, 2.20 parts by weight of sodium hyaluronate, 2.50 parts by weight of acetylated sodium hyaluronate, and 0.40 parts by weight of hydrolyzed sodium hyaluronate.

[0158] Comparative Example 7

[0159] The difference between this comparative example and Example 3 is that: the extract of Chondrus crispus and the extract of Okoume tree resin are not added to the components of Group B, and the other components and steps remain unchanged.

[0160] An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, wherein the facial mask liquid comprises components A, B, C, and D;

[0161] In terms of weight, the components of group A include the following raw materials: 18 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0162] In terms of weight, the B group includes the following raw materials: 1.60 parts of succulent red vegetable extract, 0.70 parts of Channa intestine extract, 0.60 parts of Artemisia sutchuenensis extract, 0.30 parts of Trifolium repens extract, 0.35 parts of Artemisia scoparia extract, and 0.15 parts of Aloe vera extract;

[0163] In terms of weight, the C group ingredients include the following raw materials: 20,000 parts of glycerin, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 300 parts of hyaluronic acid mixture, 1,000 parts of niacinamide, and 300,000 parts of water;

[0164] In terms of weight, the D component includes the following raw materials: 2000 parts of betaine, 200 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 300 parts of hydroxyethylpiperazine ethane sulfonic acid, 3 parts of carbomer, 300 parts of allantoin, 0.0030 parts of p-hydroxyacetophenone, and 1.00 parts of bosine.

[0165] The hyaluronic acid mixture is prepared by uniformly mixing 1.20 parts by weight of sodium hyaluronate cross-linked polymer, 2.20 parts by weight of sodium hyaluronate, 2.50 parts by weight of acetylated sodium hyaluronate, and 0.40 parts by weight of hydrolyzed sodium hyaluronate.

[0166] Comparative Example 8

[0167] The difference between this comparative example and Example 3 is that no hyaluronic acid mixture is added to the C group components, and the other components and steps remain unchanged.

[0168] In terms of weight, the C group includes the following raw materials: 20,000 parts of glycerol, 150 parts of hydrolyzed sclerotium gum, 0.001200 parts of valine, 0.75 parts of collagen, 1,000 parts of niacinamide, and 300,000 parts of water;

[0169] Comparative Example 9

[0170] The difference between this comparative example and Example 3 is that astaxanthin is not added to Group A, and the other ingredients and steps remain unchanged.

[0171] In terms of weight, the components of group A include the following raw materials: 3 parts of bifid yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0172] Comparative Example 10

[0173] The difference between this comparative example and Example 3 is that the number of astaxanthin raw materials in Group A is changed to 5 parts, and the other ingredients and steps remain unchanged.

[0174] In terms of weight, the components of group A include the following raw materials: 5 parts of astaxanthin, 3 parts of bifida yeast extract, 5 parts of lactic acid bacteria fermentation extract, 600 parts of fine algae polysaccharide, and 1800 parts of trehalose;

[0175] Experimental Example 1 Whitening Effect Test

[0176] 140 subjects aged between 22 and 35 were selected, and all subjects were divided into 13 experimental groups and 1 blank control group, each group was composed of 10 people, half of whom were male and half were female. The experimental group used the mask prepared by Examples 1 to 3 and Comparative Examples 1 to 10, respectively, and the blank control group used clean water to wash the face. The test period was 30 days in total, used twice a day, and the mask was attached to the face for 10 minutes after cleansing. No other skin care products were used during the test. ITA was tested using a skin colorimeter and a skin melanin detector before use (0d), on the 14th day (14d) and on the 30th day (30d). , skin brightness (L value) and melanin index. Among them, ITA. The parameters of human skin color are characterized by measuring the skin L*a*b* color space data by a skin colorimeter. The larger the value, the brighter the skin, and vice versa, the darker the skin. The melanin index characterizes the melanin content in the skin by measuring the absorption of a specific wavelength spectrum by the skin surface. The higher the melanin content, the darker the skin color. The above three indicators are the average of the test results within each experimental group.

[0177] Statistical analysis software was used for statistical analysis of the data. Measurement data were expressed as mean ± standard deviation, and normal distribution test was performed. If the data met the normal distribution requirements, the paired t test was used for comparison before and after use, otherwise the two related sample rank sum test was used; the comparison of the rank data before and after use was done with two related sample rank sum test; the comparison between the test product and the control group was done with independent sample t test or rank sum test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. When the test value after using the sample was significantly better than that of the control group (P < 0.05), the test product was deemed to have the effect of removing freckles and whitening, otherwise it was deemed that the test product had no effect of removing freckles and whitening.

[0178] The test results are shown in Table 1, Table 2 and Table 3:

[0179] Table 1 Changes in skin melanin before and after using the mask

[0180]

[0181] (Note: Comparison between experimental group and blank control group, *P<0.05, **P<0.01)

[0182] The results of the test on the content of melanin in the skin are shown in Table 1. The content of melanin in the skin of the experimental groups (Examples 1 to 3 and Comparative Examples 1 to 10) gradually decreased during the test period, especially Examples 1 to 3 showed significant and extremely significant differences compared with the blank control group after using the product for 14 days (P < 0.05) and 30 days (P < 0.01). The Comparative Examples 1 to 10 showed significant differences compared with the blank control group after using the product for 30 days (P < 0.01), and the content of melanin in the skin of the blank control group was relatively stable during the test period, indicating that the prepared facial mask liquid had a certain whitening effect during the test period, especially the facial mask liquid prepared in Example 3 had the best whitening effect.

[0183] Table 2 Skin brightness L* value test results before and after using the mask

[0184]

[0185] The skin L* value represents the brightness of the skin. The larger the value, the more the skin color tends to be white, and vice versa. As shown in Table 2, the L* value of the facial mask liquid prepared in Example 3 is the highest.

[0186] Table 3 Changes in skin ITA values

[0187]

[0188]

[0189] (Note: Comparison between experimental group and blank control group, *P<0.05, **P<0.01)

[0190] As can be seen from Table 3, the skin ITA values ​​of Examples 1 to 3 and Comparative Examples 1 to 10 gradually increased during the test period. There were significant differences between Examples 1 to 3 and the blank control group after 14 days of use (P < 0.05), and there were extremely significant differences between Examples 1 to 3 and the blank control group after 30 days of use (P < 0.01). The skin ITA values ​​of the blank control group increased slightly during the test period and were relatively stable. At 30 days, there were significant differences between Comparative Examples 1, 2, 8 and 10 and the blank control group, indicating that the facial mask liquid prepared by the present invention showed a certain whitening effect during the test period.

[0191] In summary, it can be seen from Table 1, Table 2 and Table 3 that the whitening effect obtained by using the specific components and component ratios in Example 3 is the best, and the active ingredients in the succulent red vegetable extract, the chapped fish intestine extract, the southern artemisia extract, the white clover extract, the artemisia spatholobi extract, the crisp wave chondrus extract, the okoume tree resin extract and the aloe extract can reduce the melanin content in the skin, making the skin white and shiny as a whole. The mask prepared by using the specific ingredients of the present invention can promote the metabolism of the melanin that has been generated, and has a strong inhibitory effect on tyrosinase, further reducing the melanin content in the skin so that the whiteness and brightness of the skin are further improved. From the comparison between Example 3 and Comparative Example 1, it can be seen that the enzymatic hydrolysis of the red cabbage extract can promote the release of intracellular active substances and improve the whitening effect; from the comparison between Example 2 and Comparative Example 2, it can be seen that the medicinal effects of Artemisia australis extract and Artemisia annua extract are similar, but the present technical scheme is selective for Artemisia australis extract, and only by adding Artemisia australis extract can the overall effect of the mask be improved; from the comparison between Example 3 and Comparative Examples 3 to 9, it can be seen that the whitening effect achieved by Example 3 is the best. If any plant extract component, hyaluronic acid or astaxanthin in Group B is missing, it will have a huge adverse effect on the final effect; from the comparison between Example 3 and Comparative Example 10, it can be seen that the present technical scheme has strict control over the fraction of astaxanthin added. If the added amount is too low, a good whitening effect cannot be achieved.

[0192] Experimental Example 2 Moisturizing Effect Test

[0193] 130 subjects were selected, aged between 22 and 35 years old. The tested parts of the subjects had not received skin treatment, beauty treatment and other tests that may affect the results. They had no active allergic diseases and had not used hormone drugs and immunosuppressants in the past month. All subjects were divided into 13 experimental groups, each of which was composed of 10 people, half of whom were male and half were female. Each experimental group used the facial mask prepared by Examples 1 to 3 and Comparative Examples 1 to 10. The test time was 40 minutes in total. After cleansing, the initial (0min) facial skin moisture was tested with a skin moisture test probe. The facial mask was then applied to the face for 10 minutes, and the mask was removed. The subjects sat in a laboratory at a temperature of 21±1°C and 50±5wt%RH, and the facial skin moisture was measured at 20min and 40min. No other skin care products were used during the test. The test results are shown in Table 4:

[0194] Table 4 Moisturizing effect before and after using facial mask

[0195]

[0196] As can be seen from Table 4, the moisturizing effect obtained by using the specific components and component ratios in Example 3 is the best. The skin moisture content corresponding to the test subjects using the mask prepared by Examples 1 to 3 is higher than the skin moisture content corresponding to the test subjects using the mask prepared by Comparative Examples 1 to 10, which shows that Test Examples 1 to 3 have a better effect of quickly replenishing facial skin moisture than Comparative Examples 1 to 10. Further, 40 minutes after using Examples 1 to 3, the rate of change of the moisture content of the test subject's skin is low and still maintained at a high level, while the test subjects using Comparative Examples 3 to 9 have a large decrease in the moisture content of the skin after 40 minutes, and there is not much improvement relative to the initial skin moisture content, especially the skin moisture content of Comparative Example 9 after 40 minutes is basically the same as the skin moisture content at 0 minutes, and the skin moisture content of the test subjects of Comparative Examples 1, 2 and 8 is lower than that of the test subjects of Examples 1 to 3. In summary, it is shown that the rapid hydration and moisturizing effects of Examples 1 to 3 are better than Comparative Examples 1 to 10. The main reason is that this application simultaneously uses a mixture of red cabbage extract, snakehead extract, Artemisia australis extract, Trifolium repens extract, Artemisia spatholobi extract, Chondrus crispus extract, Okoume tree resin extract, aloe extract, astaxanthin and hyaluronic acid within a specific proportion to cooperate with each other to synergistically promote the moisturizing effect of the mask.

[0197] Experimental Example 3 Anti-aging effect - DPPH and -OH free radical scavenging ability

[0198] 3.1 Test methods

[0199] (1) Determination of the ability to scavenge 1,2-diphenylpicrylhydrazyl free radical (DPPH-): Use anhydrous ethanol to prepare 2 mg / mL mask liquid and 0.2 mmol / L DPPH solution, take 2 mL of mask liquid and DPPH solution in test tubes respectively, shake well and let stand at room temperature for 30 minutes, the absorbance is A1; at the same time, measure the absorbance after 2 mL DPPH solution mixed with 2 mL distilled water A2 and the absorbance after 2 mL mask liquid mixed with 2 mL anhydrous ethanol A3, and avoid light reaction. Measure the absorbance at 517 nm, and the scavenging rate is calculated as:

[0200]

[0201] (2) Determination of the ability to scavenge hydroxyl (-OH) free radicals: The test adopts the salicylic acid method. 2 mL of 9 mmol / L FeSO4, 2 mL of 9 mmol / L salicylic acid, 2 mL of ethanol, 2 mL of facial mask liquid, and 2 mL of 8.8 mmol / L H2O2 are added to the colorimetric tube in sequence. H2O2 starts the entire reaction. After reacting at 37°C for 0.5 h, the sample absorbance A1 is measured at 510 nm. Distilled water is used instead of facial mask liquid as a blank to adjust the absorbance A0. The formula is:

[0202]

[0203] 3.2 Test results

[0204] The results are shown in Table 5:

[0205] Table 5 DPPH and -OH free radical scavenging ability

[0206]

[0207]

[0208] As can be seen from Table 5, the anti-aging effect obtained by using the specific components and component ratios in Example 3 is the best. The facial mask liquid prepared in the present application has a good anti-aging effect, can consume the harmful compound free radicals produced in the body's oxidation reaction, reduce damage to the body's tissues and cells, and slow down aging; Examples 1 to 3, Comparative Examples 1 to 8 and Comparative Example 10 (all with added astaxanthin) have higher DPPH and -OH free radical scavenging abilities than Comparative Example 9 (without added astaxanthin), indicating that astaxanthin can increase the antioxidant capacity of the facial mask liquid and slow down skin aging. Other plant component extracts and hyaluronic acid can synergize with astaxanthin to improve its antioxidant capacity.

[0209] Experimental Example 4: Evaluation of Soothing Effect

[0210] 4.1 Experimental Principle: Current research suggests that the occurrence of sensitive skin is a complex process involving skin barrier, nerves and blood vessels, and immune inflammation. Hyaluronic acid is the component with the highest content and the largest proportion in the extracellular matrix. It can maintain the volume of the extracellular matrix, regulate the secretion of cell growth factors and cytokines, and affect cell adhesion, growth, proliferation and differentiation. Therefore, it plays an important role in maintaining skin moisture and elasticity, wound healing and angiogenesis.

[0211] Hyaluronidase is an endogenous aminohexosidase that causes the decomposition of macromolecular hyaluronic acid. It is strongly correlated with inflammation and allergies. Studies have reported that various drugs that release histamine from mast cells can regulate hyaluronidase activity. Some anti-allergic drugs have strong inhibitory effects on hyaluronidase activity. Therefore, inhibition of hyaluronidase activity is used as an indicator for studying anti-allergic effects. Macromolecular hyaluronic acid is decomposed to produce N-acetylglucosamine under the action of hyaluronidase. Determining the content of N-acetylglucosamine in the reaction system can indirectly reflect the activity of hyaluronidase.

[0212] 4.2 Evaluation Metrics

[0213] If the test sample has a certain inhibition rate on hyaluronidase, it can be considered that the test sample has a certain soothing effect. The higher the hyaluronidase activity inhibition rate, the stronger the soothing effect of the substance.

[0214] 4.3 Test Method

[0215] Hyaluronidase inhibition test Elson-Morgan modified method: take the test sample stock solution (mask solution) → perform color reaction between the sample and hyaluronidase solution → test the absorbance at 530nm → calculate the hyaluronidase activity inhibition rate.

[0216] 4.4 Test Results

[0217] As shown in Table 6:

[0218] Table 6 Hyaluronidase inhibition rate

[0219]

[0220] As can be seen from Table 6, the soothing effect obtained by using the specific components and component ratios in Example 3 is the best. The samples in Examples 1 to 3 all have a significant inhibitory effect on hyaluronidase. The present invention uses astaxanthin and plant extracts including red cabbage extract, snakehead intestine extract, artemisia australis extract, white clover extract, artemisia spatholobi extract, crispa extract, okoume resin extract and aloe extract to synergize with each other to inhibit the release of histamine from the source, and can effectively inhibit the production of pro-inflammatory factors (IL-1, IL-6, TNF-α), thereby having a higher skin soothing function, significantly improving the inhibition rate of hyaluronidase, and having a higher skin soothing function. It can be seen from Example 3 and Comparative Example 2 that adding Artemisia annua extract to the mask liquid has a better effect than adding Artemisia annua extract. The Artemisia annua extract contains active ingredients such as sesquiterpene lactones, coumarins and thujone, which can effectively inhibit the production of pro-inflammatory factors; it can be seen from Example 3 and Comparative Examples 3 to 7 and Comparative Example 10 that only by adding all the plant extracts of the present application in a suitable proportion can the best effect be achieved; the comparison of the results of Example 3 and Comparative Example 8 shows that adding a hyaluronic acid mixture to the mask liquid has a synergistic and enhanced effect, so that the inhibition rate of the sample on hyaluronidase is doubled, the release of histamine is inhibited from the source, and the anti-allergic and soothing effect is good. It can be seen from the comparison of Example 3 and Comparative Example 9 that adding astaxanthin can have a soothing effect.

[0221] Experimental Example 5: Evaluation of Anti-wrinkle Effect

[0222] 5.1 Experimental Principle: Elastase is a highly selective and specific proteolytic enzyme that catalyzes the hydrolysis of many carboxyl-containing polypeptide bonds and can decompose elastin in connective tissue proteins. Elastase inhibitors can effectively inhibit the activity of elastase, slow down the degradation of elastin, and play a role in delaying aging and reducing wrinkles.

[0223] In this experiment, N-Succinyl-Ala-Ala-Ala-p-nitroa nilide was used as the substrate. Porcine pancreatic elastase catalyzed the reaction with the substrate. After the active substance was added, the absorbance changed. The magnitude of the absorbance change reflected the inhibition rate of the elastase inhibitor.

[0224] 5.2 Evaluation Metrics

[0225] Calculate the inhibition rate of elastase after adding the test substance;

[0226] 5.3 Test Method

[0227] Refer to "Evaluation Method for Anti-wrinkle Efficacy of Cosmetics [J]. Daily Chemicals Science" by Liu Yang et al.: Take the test sample stock solution, incubate the sample with elastase solution at 25°C for a period of time, then add the substrate and continue incubation, test the absorbance of elastase on the machine, and calculate the inhibition rate.

[0228] 5.4 Test Results

[0229] The test results are shown in Table 7:

[0230] Table 7 Elastase inhibition rate

[0231]

[0232] As can be seen from Table 7, the best anti-wrinkle effect is obtained by using the specific components and component ratios in Example 3. The facial masks prepared in Examples 1 to 3 of the present invention have anti-wrinkle effects, especially the best anti-wrinkle effect in Example 3. The facial mask prepared according to the components and ratios in this technical solution can achieve the best anti-wrinkle effect, and achieve skin anti-wrinkle by promoting collagen regeneration, inhibiting sebaceous gland secretion and improving skin microcirculation; from the comparison between Example 3 and Comparative Example 9, it can be seen that astaxanthin has a significant anti-wrinkle effect.

[0233] Experimental Example 6 Evaluation of Anti-Acne Efficacy

[0234] 6.1 Acne, also known as juvenile acne, is a chronic inflammation of the hair follicles and sebaceous glands. It usually occurs in oily areas of the skin, such as the forehead, bridge of the nose, and back of the chest. It is self-limiting, with various lesions, such as comedones, papules, pustules, and nodules, often accompanied by seborrhea. Acne is a multifactorial skin disease. It is mainly related to increased sebum production, hyperkeratosis of the epithelium at the mouth of the hair follicle, and proliferation of Propionibacterium acnes in the hair follicles.

[0235] 6.2 Test method: With reference to Appendix C4 of "GB15979 2002 Hygienic Standard for Disposable Sanitary Products" for the test method for antibacterial performance of dissolution antibacterial (inhibitory) products, the mask liquids of Examples 1 to 3 and Comparative Examples 1 to 10 were tested for their inhibition rates against Propionibacterium acnes (ATCC 6919) and Staphylococcus aureus (ATCC 12228).

[0236] 6.3 Test Results

[0237] The test results are shown in Table 8:

[0238] Table 8 Inhibition rate of facial mask liquid on Propionibacterium acnes and Staphylococcus aureus

[0239]

[0240] As shown in Table 8, the acne removal effect of the specific components and component ratios in Example 3 is the best. The astaxanthin facial mask of Examples 1 to 3 of the present invention has a strong inhibitory effect on Propionibacterium acnes and Staphylococcus aureus, all reaching more than 86%, indicating an acne removal effect. From the comparison of Example 3 with Comparative Example 3 and Comparative Example 5, it can be seen that the extract of red cabbage, Artemisia spathulata extract, Artemisia scoparia extract and Trifolium repens extract play a significant inhibitory effect on Propionibacterium acnes and Staphylococcus aureus.

[0241] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An astaxanthin facial mask, obtained by immersing a facial mask matrix in a facial mask liquid, characterized in that: The facial mask liquid comprises components A, B, C and D; In terms of weight, the components of group A include the following raw materials: 12 to 20 parts of astaxanthin, 2 to 5 parts of bifida yeast extract, 1 to 10 parts of lactic acid bacteria fermentation extract, 432 to 720 parts of fine algae polysaccharide, and 1200 to 2000 parts of trehalose; In terms of weight, the B group includes the following raw materials: 1.20-2.00 parts of red cabbage extract, 0.48-0.80 parts of channa scabra extract, 0.48-0.80 parts of artemisia argyi extract, 0.24-0.40 parts of white clover extract, 0.24-0.40 parts of artemisia scoparia extract, 2.40-4.00 parts of Chondrus crispus extract, 0.12-0.20 parts of okoume resin extract, 0.12-0.20 parts of aloe extract; In terms of weight, the C group includes the following raw materials: 16800-28000 parts of glycerin, 108-180 parts of hydrolyzed sclerotium gum, 0.000744-0.001240 parts of valine, 0.48-0.80 parts of collagen, 240-400 parts of hyaluronic acid mixture, 720-1200 parts of niacinamide, and 206400-344000 parts of water; In terms of weight, the D component includes the following raw materials: 1440 to 2400 parts of betaine, 168 to 280 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 239 to 398 parts of hydroxyethylpiperazine ethane sulfonic acid, 1 to 6 parts of carbomer, 240 to 400 parts of allantoin, 0.0024 to 0.0040 parts of p-hydroxyacetophenone, and 0.30 to 1.50 parts of bosine.

2. The astaxanthin facial mask according to claim 1, characterized in that The hyaluronic acid mixture in component C is prepared by uniformly mixing 0.84 to 1.40 parts by weight of sodium hyaluronate cross-linked polymer, 1.80 to 3.00 parts by weight of sodium hyaluronate, 2.10 to 3.50 parts by weight of acetylated sodium hyaluronate, and 0.36 to 0.60 parts by weight of hydrolyzed sodium hyaluronate.

3. The astaxanthin facial mask according to claim 1, characterized in that The preparation method of the succulent red vegetable extract comprises the following steps: (1) Washing the red succulent vegetable, drying and crushing it, adding the crushed red succulent vegetable to a 5-10% by mass sodium bicarbonate aqueous solution to obtain a mixture, gradually heating the mixture to 52-58° C., stirring at a speed of 45-55 rpm for 7-10 hours, and cooling the mixture to 25-28° C. after the stirring is completed; (2) The cooled mixture is first beaten to form a slurry, and then the pH value of the slurry is adjusted to 6.8-7.8 with an acid solution, and then a composite enzyme is added to perform enzymolysis for 60-100 minutes at a hydrolysis temperature of 35-42° C. After the enzymolysis is completed, the solid-liquid separation is performed to obtain an enzymolysis solution, and the enzymolysis solution is concentrated at low temperature and reduced pressure to 60-70% of the original volume to obtain a succulent red vegetable extract.

4. The astaxanthin facial mask according to claim 3, characterized in that The succulent red vegetable and the sodium bicarbonate aqueous solution are mixed at a material-liquid ratio of 1:(15-20) g / mL, and the added amount of the complex enzyme is 0.5-3% of the total mass of the slurry.

5. The astaxanthin facial mask according to claim 3, characterized in that The complex enzyme is prepared by mixing beta-glucanase, beta-xylosidase and cellulase in a mass ratio of 1:(1-2.5):(3-7).

6. The astaxanthin facial mask according to claim 1, characterized in that The preparation method of the snakehead intestine extract comprises the following steps: crushing the snakehead intestine, adding an ethanol aqueous solution with a mass concentration of 70-80% and a solid-liquid ratio of 1:(5-10) g / mL, soaking for 80-150 minutes, refluxing and extracting at a temperature of 40-50° C. for 40-60 minutes, refluxing and extracting 2-4 times, filtering, combining the filtrate, concentrating under reduced pressure, and freeze-drying to obtain the snakehead intestine extract.

7. The astaxanthin facial mask according to claim 6, characterized in that The freeze-drying temperature is -45 to -35°C.

8. The astaxanthin facial mask according to claim 1, characterized in that The preparation method of the Artemisia argyi extract comprises the following steps: (1) mixing the chopped whole herb of Artemisia australis and anhydrous ethanol in a mass ratio of 1:(8-12), stirring at a temperature of 50-55° C. for 30-60 minutes to obtain an initial mixture; (2) filtering the initial mixture for the first time to obtain a first filtrate; (3) refrigerating the first filtrate at a temperature of 1 to 4° C. for 3 to 7 hours to obtain a refrigerated liquid; (4) filtering the refrigerated liquid for a second time to obtain a second filtrate and solid residue; (5) placing the second filtrate under vacuum conditions of 0.04-0.08 MPa and a temperature of 60-65° C. for evaporation concentration for 25-35 min to obtain a concentrate; (6) mixing the solid residue and the concentrate and drying them to obtain an Artemisia argyi extract.

9. The method for preparing the astaxanthin facial mask according to claim 1, characterized in that, The steps include: S1. Add the C group components into the emulsifying pot, stir and heat to 70-80℃ to dissolve evenly; S2. Add the components of group D into the emulsifying pot after stirring and dispersing evenly, and cool to 30-40°C; S3, stirring and dissolving the components of group A and group B respectively and adding them into the emulsifying pot to obtain a facial mask liquid; S4. Soak the mask matrix in the mask liquid to obtain the astaxanthin mask.

10. Use of the astaxanthin mask according to any one of claims 1 to 8 or the astaxanthin mask prepared by the preparation method according to claim 9 in preparing skin care products for keeping skin firm and anti-wrinkle, moisturizing, whitening, anti-oxidation, removing acne and soothing the skin.

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