Wound plaster and preparation method thereof

Abstract

The invention discloses a wound plaster which comprises borneol, pearl powder, angelica sinensis, modified silver sulfadiazine, coptis chinensis, liquorice and bletilla striata, the mass ratio of the borneol to the pearl powder to the angelica sinensis to the coptis chinensis to the liquorice to the bletilla striata is (1-2): (0.5-1): (1-2): (1-2): (2-3): (0.5-1), and the addition amount of the modified silver sulfadiazine accounts for 5-8% of the total mass of the borneol, the bletilla striata, dragon's blood and the coptis chinensis. By optimizing extraction process parameters, such as temperature, time and solvent proportion, extraction is ensured to be performed under optimal conditions, so that the extraction rate is improved, the yield can be increased, the waste of raw materials can be reduced, the production cost is reduced, the economic benefit is improved, heat clearing, detoxification, anti-inflammation, analgesia and cell regeneration are taken as treatment criteria, and the treatment cost is reduced. Various medicinal materials are combined and cooperated, the synergistic effect of all functional organs of the body is adjusted from outside to inside, the skills of the human body are stimulated, wound healing is promoted, and the modified aloe extracting solution and the modified dandelion extracting solution in the mixture can enhance the antibacterial effect of silver sulfadiazine to generate the synergistic or antagonistic effect.

Description

Technical Field

[0001] The invention relates to the technical field of wound surface medicine preparation, in particular to a wound surface plaster and a preparation method thereof. Background Art

[0002] Wounds generally refer to tissue damage caused by heat, including hot liquids (water, soup, oil, etc.), steam, high-temperature gas, flame, hot metal liquid or solid (such as molten steel, steel ingots), strong radioactive radiation, etc., mainly referring to the skin and / or mucous membranes. In severe cases, subcutaneous and / or submucosal tissues such as muscles, bones, joints and even internal organs may also be injured. Scalds are tissue damage caused by hot liquids, steam, etc., and are a type of thermal burns. China's Nine-Point Method: Proposed by the Third Military Medical University of the Chinese People's Liberation Army, the adult body surface is divided into 11 equal parts, including 9% of the head, face and neck, 2 9% of the upper limbs, 3 9% of the front and back of the trunk (each 13%) and the perineum (1%), and 5 9% + 1% (46%) of the lower limbs including the buttocks.

[0003] The most critical part of treating burns and scalds is the treatment of the wound surface. Improper treatment will lead to complications such as wound infection and organ damage, and even serious enough to cause the patient's death. Therefore, the effective way to treat burns and scalds is to prevent infection and promote wound healing. Most Western medicine treatments for burns and scalds use antibiotics and analgesics, which are effective, but have certain side effects, and antibiotics are prone to drug resistance, which increases the difficulty of treatment. The history of traditional Chinese medicine in treating burns and scalds is long. The use of ointments for treatment began in the pre-Qin period. In recent years, traditional Chinese medicine has become increasingly prominent in the treatment of burns and scalds with its advantages of multiple targets and multiple mechanisms. Studies have shown that traditional Chinese medicine treatment has a certain scientific basis, can promote the growth of skin tissue, has anti-inflammatory, anti-infective, and antioxidant effects, inhibits scar hyperplasia, and relieves pain. However, although traditional Chinese medicine has many advantages, the extraction rate of traditional Chinese medicine when it is made into plasters is a major pain point.

[0004] Common Western medicines such as silver sulfadiazine cream can prevent and treat secondary wound infections caused by small-area, mild burns, but the effects are limited. If you want to treat large-area burns, the cost of medicine and production will also increase. Summary of the invention

[0005] In order to solve the above-mentioned deficiencies in the prior art, the present invention provides a wound surface plaster and a preparation method thereof.

[0006] In order to solve the above technical problems, the present invention adopts the following technical scheme: a wound plaster, comprising borneol, pearl powder, angelica, modified silver sulfadiazine, coptis, licorice, and bletilla striata, the mass ratio of borneol, pearl powder, angelica, coptis, licorice, and bletilla striata is 1-2:0.5-1:1-2:1-2:2-3:0.5-1, and the added amount of modified silver sulfadiazine is 5-8% of the total mass of borneol, bletilla striata, dragon's blood, and coptis.

[0007] A method for preparing a wound plaster comprises the following steps:

[0008] Preparation of mixed solution: Weigh borneol, Bletilla striata and Coptis chinensis according to the proportion, grind into powder and mix evenly, add 50-60% ethanol solution for soaking, soaking time is 30-40 hours, stirring is required at irregular intervals during the soaking, and then heat and reflux for 1.5-2.5 hours under ultrasonic conditions, extract 3-5 times in total, cool, combine the extracts, put the extracts into a rotary evaporator to separate ethanol, and heat and stir the extracts after removing ethanol until it becomes viscous to obtain a mixed solution;

[0009] Preparation of concentrated solution: Weigh angelica and licorice according to the proportion, grind into powder and mix evenly, add into cold water and soak, let stand for 35-48 hours, keep the cold water temperature unchanged during the standing process, after the standing is completed, filter to obtain the filtrate and product A;

[0010] Turn on the high-pressure pump and the extractor, put the filtrate into the extractor, compress the carbon dioxide gas to 100-300 bar, start the high-pressure pump, and let the supercritical carbon dioxide flow into the extractor for extraction. After the extraction is completed, the supercritical fluid containing dissolved components is reduced to below the critical point, so that the dissolved effective components are precipitated. The collected extract can be concentrated by rotary evaporation or the like, and the remaining carbon dioxide is removed to obtain a concentrated solution;

[0011] Enzymolysis: Pour room temperature ionized water into the pearl powder and mix and stir, adjust the pH value to 5-6, add compound enzyme for enzymolysis at 30-40℃, heat to inactivate the enzyme after the enzymolysis is completed, separate the liquid part and the solid residue, mix the liquid part with product A and stir, add acetic acid to adjust the pH value to 5-6 after stirring, add compound enzyme for enzymolysis at 30-40℃, after the enzymolysis is completed, use low temperature treatment to inactivate the enzyme, separate and obtain the mixed extract;

[0012] Preparation of wound plaster: Mix the mixed solution, concentrated solution, mixed extract and modified silver sulfadiazine at a temperature of 0-1°C, make a paste after stirring, and store in a refrigerator to obtain a wound plaster.

[0013] The present invention optimizes the extraction process parameters, such as temperature, time, solvent ratio, etc., to ensure that the extraction is carried out under the best conditions, thereby improving the extraction rate. By utilizing the multi-stage extraction process, we can ensure that the effective ingredients are extracted from various parts of the medicinal materials, realize full-component extraction, and comprehensively extract a variety of active ingredients in traditional Chinese medicines to ensure that no important pharmacological substances are missed. In the preparation of the concentrated solution, the heat-sensitive components in the traditional Chinese medicines are first extracted by cold immersion. Similarly, after the pearl powder is enzymatically hydrolyzed to extract most of the components, the remaining filter residue and the remaining A product are subjected to secondary extraction by enzymatic hydrolysis to improve the extraction rate and purity. While achieving a high extraction rate, we strictly control the extraction process to ensure the purity and quality of the extract to meet the pharmacopoeia standards.

[0014] Preferably, the preparation method of modified silver sulfadiazine comprises:

[0015] S1. Place sulfadiazine in an ethanol solution and stir until completely dissolved. The ratio of sulfadiazine to 65-75% ethanol is 0.5-1.5 g:10 ml.

[0016] S2, dissolving silver nitrate in distilled water to form a silver nitrate solution;

[0017] S3, slowly adding the silver nitrate solution to the ethanol solution of sulfadiazine, and during the mixing process, the pH value is controlled within 6-8;

[0018] S4. Using vacuum filtration or a centrifuge to separate the precipitate from the reaction solution of the mixed solution obtained in S3, and washing the precipitate with distilled water for multiple times to remove unreacted reagents and by-products, thereby obtaining modified silver sulfadiazine.

[0019] Preferably, in the preparation method S3 of modified silver sulfadiazine, at a temperature of 0-1°C, the modified dandelion extract and the modified aloe extract are slowly added to the mixed solution after mixing and stirred. During the mixing process, citric acid is added to ensure that the pH value is maintained within 3 during the mixing process. The dropping speed is 1-2 / s. After the addition is completed, the surface modifier is added and stirred. After the stirring is completed, the precipitate is separated from the reaction solution by vacuum filtration or centrifuge, and the precipitate is washed with distilled water for multiple times to remove unreacted reagents and by-products to obtain modified silver sulfadiazine. The modified silver sulfadiazine obtained is the final product.

[0020] Preferably, the preparation method of the modified dandelion extract comprises:

[0021] Grind dandelion and tea leaves into powders according to a certain proportion and mix them evenly; add 60-80% ethanol solution and soak them at room temperature; the mass ratio of the mixed powder of dandelion and tea leaves to the ethanol solution is 1:5; the soaking time is 30-40 hours; during this period, stirring is required from time to time; heat and reflux for 1.5-2.5 hours; extract 3-5 times in total; cool, filter, and concentrate under reduced pressure to obtain a concentrated extract; add citric acid dropwise to the concentrated extract and stir until the pH value reaches 3 and the addition is completed to obtain a modified dandelion extract.

[0022] Preferably, the preparation method of the modified aloe extract comprises:

[0023] Aloe vera and tea powder are mixed evenly in proportion, and an ethanol solution with a concentration of 60-80% is added for soaking at room temperature. The mass ratio of the mixed powder of aloe vera and tea to the ethanol solution is 1:5. The soaking time is 30-40 hours, during which irregular stirring is required. The mixture is heated under reflux for 1.5-2.5 hours, and extracted 3-5 times in total. The mixture is cooled, filtered, and concentrated under reduced pressure to obtain a concentrated extract. Citric acid is added dropwise to the concentrated extract and stirred until the pH value reaches 3 and the addition is completed to obtain a modified aloe vera extract.

[0024] Preferably, in the enzymatic hydrolysis step, the complex enzyme is one or more of xylanase, cellulase, protease and hemicellulase.

[0025] Preferably, in the modified silver sulfadiazine, the mass ratio of sulfadiazine and silver nitrate is 1:1, the added amount of the modified dandelion extract is 4-7% of the total mass of sulfadiazine and silver nitrate, the added amount of the modified aloe extract is 2-5% of the total mass of sulfadiazine and silver nitrate, and the added amount of the modified modifier is 1-3% of the total mass of sulfadiazine and silver nitrate.

[0026] Preferably, in the method for preparing the modified dandelion extract, the mass ratio of dandelion to tea leaves is 2:1, and the mass ratio of aloe vera to tea leaves powder is 3:1.

[0027] Borneol has the functions of clearing away heat and detoxifying, relieving pain and itching, helping to reduce swelling, blood stasis and swelling and pain, having certain antibacterial and anti-inflammatory effects, helping to treat infectious diseases, and promoting blood circulation, helping to improve poor local blood circulation;

[0028] Pearl powder is rich in calcium and a variety of trace elements, which helps to inhibit the production of melanin and promote skin metabolism. It contains a variety of amino acids and trace elements, which can promote cell regeneration and enhance skin elasticity. It has good moisturizing properties and can maintain skin moisture. Pearl powder has a certain anti-inflammatory effect and can relieve skin inflammation and redness and swelling symptoms. Pearl powder helps promote wound healing and reduce scar formation. Pearl powder has the function of regulating the immune system, which can enhance the body's resistance and improve immunity.

[0029] Angelica has a significant blood-tonifying effect, helps improve blood circulation, and promotes cell regeneration. Angelica contains a variety of antioxidant ingredients that can fight free radicals, have anti-inflammatory effects, and help promote wound healing and reduce scar formation.

[0030] Coptis chinensis has a significant effect of clearing heat and drying dampness, and can relieve fire and detoxify. It has an inhibitory effect on many bacteria and viruses. Berberine, the main ingredient in Coptis chinensis, has a broad-spectrum antibacterial effect and has an inhibitory effect on many bacteria such as Staphylococcus aureus, Streptococcus, Escherichia coli, etc. Coptis chinensis has a certain sedative effect and reduces pain perception.

[0031] Licorice is often used in traditional Chinese medicine prescriptions. It can harmonize the medicinal properties of other medicinal materials, enhance the effect of the prescription, reduce the side effects of other drugs, has the effect of clearing away heat and detoxifying, can relieve the symptoms caused by fever, can be used for anti-inflammation and detoxification, promote wound healing, and has a certain analgesic effect. Certain components in licorice have anti-allergic effects, which can prevent users from being allergic to certain medicinal materials in the medicine.

[0032] Bletilla striata has a good hemostatic effect and can promote wound healing. Bletilla striata has a certain anti-inflammatory effect and can help reduce the inflammatory response. It has the effect of clearing heat and detoxifying, and can relieve the symptoms caused by heat and toxins.

[0033] Silver sulfadiazine has the antibacterial effect of sulfadiazine and the astringent effect of silver salt. It has good antibacterial activity against most Gram-positive and Gram-negative bacteria, and can promote wound drying, scabbing and healing. It is mainly used to prevent and treat secondary wound infection of small-area, mild burns and scalds. It can prevent and control infection, and can also promote wound drying, scabbing and healing.

[0034] The main ingredients of sulfadiazine silver are sulfadiazine and silver ions. Sulfadiazine is an antibacterial agent, and silver ions have strong antibacterial and anti-inflammatory effects. Aloe extract contains a variety of bioactive ingredients, such as polysaccharides, amino acids, flavonoids, phenolic compounds, organic acids and vitamins, which have anti-inflammatory, antibacterial, moisturizing and wound healing effects.

[0035] The modified aloe extract and modified dandelion extract in the mixture can enhance the antibacterial effect of silver sulfadiazine. The antibacterial effect of the modified aloe extract and modified dandelion extract themselves can produce a synergistic or antagonistic effect with silver sulfadiazine, effectively reducing the inflammatory response around the wound. The modified aloe extract and modified dandelion extract can reduce the local irritation of silver sulfadiazine, thereby improving the patient's tolerance. Both the modified aloe extract and the modified dandelion extract have significant anti-inflammatory effects. When combined with silver ions with anti-inflammatory effects, they may enhance the overall anti-inflammatory effect and help wound healing. However, the phenolic compounds in the aloe extract will form complexes with silver ions, thereby affecting the antibacterial activity of silver ions. This complex may reduce the solubility and bioavailability of silver ions, but silver ions (Ag+) usually do not react directly with phenolic compounds in an acidic environment and at low temperatures, which can minimize the loss of silver ions and ensure the antibacterial activity of silver sulfadiazine. After silver sulfadiazine and modified aloe extract and modified dandelion extract are mixed, a surface modifier is added. On the metal surface, the surface modifier can form a protective layer to prevent corrosion, and a dense oxide film is formed on the surface of silver sulfadiazine, which enhances the stability of silver ions and reduces the chemical reaction between silver ions and other substances.

[0036] Dandelion extract and tea polyphenols have antioxidant, anti-inflammatory and antibacterial effects. The addition of citric acid helps to improve the overall antibacterial effect, especially in an acidic environment. In addition, tea polyphenols can enhance the antioxidant capacity of the extract and produce a synergistic effect. The addition of citric acid will lower the pH value of the mixture, thereby inhibiting the growth of most microorganisms and helping to extend the shelf life.

[0037] Both aloe vera extract and tea polyphenols have strong antioxidant properties and can work synergistically to enhance the antioxidant effect. Citric acid, as an antioxidant, can also enhance this effect. Aloe vera extract has certain antibacterial and anti-inflammatory effects, and tea polyphenols also have strong antibacterial and antioxidant effects. Although citric acid is not the main antibacterial agent, its acidic environment can enhance the antibacterial effects of both. The mixed solution can be used for skin care and has moisturizing, antioxidant and anti-inflammatory effects. The moisturizing effect of aloe vera extract, the antioxidant effect of tea polyphenols, and the slight exfoliating effect of citric acid can work synergistically to improve skin conditions.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] 1. The present invention takes clearing away heat and detoxification, anti-inflammatory and analgesic, and cell regeneration as the treatment principles. A variety of medicinal materials are combined synergistically to regulate the synergistic effects of various functional organs of the body from the outside to the inside, stimulate human skills, and promote wound healing.

[0040] 2. The present invention optimizes the extraction process parameters, such as temperature, time, solvent ratio, etc., to ensure that the extraction is carried out under the best conditions, thereby improving the extraction rate, which can not only increase the output, but also reduce the waste of raw materials, reduce production costs, and improve economic benefits.

[0041] 3. The modified aloe extract and modified dandelion extract in the mixture can enhance the antibacterial effect of silver sulfadiazine. The antibacterial effect of the modified aloe extract and modified dandelion extract themselves can produce a synergistic or antagonistic effect with silver sulfadiazine, effectively reducing the inflammatory response around the wound. The modified aloe extract and modified dandelion extract can reduce the local irritation of silver sulfadiazine, thereby improving the patient's tolerance, while reducing the reaction between silver ions and other substances to enhance the antibacterial activity of silver ions. DETAILED DESCRIPTION

[0042] The invention is described in detail below with reference to the embodiments.

[0043] Example 1

[0044] Prescription: including borneol, pearl powder, angelica, modified silver sulfadiazine, coptis, licorice, and bletilla striata. The mass ratio of borneol, pearl powder, angelica, coptis, licorice, and bletilla striata is 1:0.5:1:12:2:0.5. The added amount of modified silver sulfadiazine is 5% of the total mass of borneol, bletilla striata, dragon's blood, and coptis.

[0045] A preparation method of a wound plaster comprises the following steps: preparing a mixed solution: weighing borneol, bletilla striata and coptis root according to a proportion, grinding them into powder and mixing them evenly, adding a 50% ethanol solution for soaking, the soaking time is 30 hours, stirring is required at irregular intervals during the soaking, heating and refluxing for 1.5 hours under ultrasonic conditions, extracting 3 times in total, cooling, combining the extracts, putting the extracts into a rotary evaporator to separate ethanol, and heating and stirring the extracts after removing ethanol until a viscous mixed solution is obtained;

[0046] Preparation of concentrated solution: Weigh angelica and licorice according to the proportion, grind into powder and mix evenly, add into cold water and soak, the cold water should be 1-2cm above the medicine, let it stand for 35-48h, keep the cold water temperature unchanged during the standing process, after the standing is over, filter to obtain the filtrate and product A;

[0047] Turn on the high-pressure pump and the extractor, put the filtrate into the extractor, compress the carbon dioxide gas to 100 bar, start the high-pressure pump, and let the supercritical carbon dioxide flow into the extractor for extraction. After the extraction is completed, the supercritical fluid containing dissolved components is reduced to below the critical point, so that the dissolved effective components are separated out. The collected extract can be concentrated by rotary evaporation or the like, and the remaining carbon dioxide is removed to obtain a concentrated solution;

[0048] Enzyme hydrolysis: Pour room temperature ionized water into the pearl powder and mix and stir, adjust the pH value to 5, add compound enzyme at 30℃ for enzymolysis, heat to inactivate the enzyme after enzymolysis, separate the liquid part and solid residue, mix the liquid part with product A and stir, add acetic acid to adjust the pH value to 5 after stirring, add compound enzyme at 30℃ for enzymolysis, inactivate the enzyme by low temperature treatment after enzymolysis, separate and obtain mixed extract, the compound enzyme is cellulase;

[0049] Preparation of wound plaster: Mix the mixed solution, concentrated solution, mixed extract and modified silver sulfadiazine at 0°C, make a paste after stirring, and store in a refrigerator to obtain a wound plaster.

[0050] The preparation method of modified silver sulfadiazine comprises:

[0051] S1. Place sulfadiazine in an ethanol solution and stir until completely dissolved. The ratio of sulfadiazine to 65% ethanol is 0.5 g:10 ml.

[0052] S2, dissolving silver nitrate in distilled water to form a silver nitrate solution;

[0053] S3, slowly adding the silver nitrate solution to the ethanol solution of sulfadiazine, during the mixing process, the pH value is controlled within 6-8, at a temperature of 0°C, slowly adding the modified dandelion extract and the modified aloe extract to the mixed solution after the mixing is completed, stirring, adding citric acid to ensure that the pH value is maintained within 3 during the mixing process, the dropping speed is 1 / s, after the addition is completed, adding the surface modifier and stirring and mixing;

[0054] S4. Use vacuum filtration or centrifuge to separate the precipitate from the reaction solution of the mixed solution obtained in the above S3, wash the precipitate with distilled water for multiple times to remove unreacted reagents and by-products, and obtain modified silver sulfadiazine; in the modified silver sulfadiazine, the mass ratio of sulfadiazine to silver nitrate is 1:1, the added amount of modified dandelion extract is 4% of the total mass of sulfadiazine and silver nitrate, the added amount of modified aloe extract is 2% of the total mass of sulfadiazine and silver nitrate, and the added amount of modified modifier is 1% of the total mass of sulfadiazine and silver nitrate.

[0055] The preparation method of the modified dandelion extract comprises:

[0056] Grind dandelion and tea leaves in a mass ratio of 3:1 until the powders are evenly mixed, add a 60% ethanol solution and soak at room temperature, the mass ratio of the mixed powder of dandelion and tea leaves to the ethanol solution is 1:5, the soaking time is 30 hours, during which irregular stirring is required, heat and reflux for 1.5 hours, extract 3 times in total, cool, filter, and concentrate under reduced pressure to obtain a concentrated extract, add citric acid dropwise to the concentrated extract and stir until the pH value reaches 3, and the addition ends to obtain a modified dandelion extract.

[0057] The preparation method of the modified aloe extract comprises:

[0058] Aloe vera and tea powder are mixed evenly in a mass ratio of 2:1, and a 60% ethanol solution is added for soaking at room temperature. The mass ratio of the mixed powder of aloe vera and tea to the ethanol solution is 1:5. The soaking time is 30 hours, during which irregular stirring is required. The mixture is heated under reflux for 1.5 hours, extracted 3 times in total, cooled, filtered, and concentrated under reduced pressure to obtain a concentrated extract. Citric acid is added dropwise to the concentrated extract and stirred until the pH value reaches 3 and the addition is completed to obtain a modified aloe vera extract.

[0059] Example 2

[0060] Prescription: including borneol, pearl powder, angelica, modified silver sulfadiazine, coptis, licorice, and bletilla striata; the mass ratio of borneol, pearl powder, angelica, coptis, licorice, and bletilla striata is 1:0.5:1:1:2:0.5; the added amount of modified silver sulfadiazine is 5-8% of the total mass of borneol, bletilla striata, dragon's blood, and coptis.

[0061] A preparation method for a wound plaster comprises the following steps: preparing a mixed solution: weighing borneol, bletilla striata and coptis root according to a proportion, grinding them into powder and mixing them evenly, adding a 60% ethanol solution for soaking, the soaking time is 35 hours, stirring is required at irregular intervals during the soaking, heating and refluxing for 1.5 hours under ultrasonic conditions, extracting 3 times in total, cooling, combining the extracts, putting the extracts into a rotary evaporator to separate ethanol, and heating and stirring the extracts after removing ethanol until a viscous mixed solution is obtained;

[0062] Preparation of concentrated solution: Weigh angelica and licorice according to the proportion, grind into powder and mix evenly, add into cold water and soak, the cold water should be 2cm above the medicine, let it stand for 40h, keep the cold water temperature unchanged during the standing process, after the standing is over, filter to obtain the filtrate and product A;

[0063] Turn on the high-pressure pump and the extractor, put the filtrate into the extractor, compress the carbon dioxide gas to 250 bar, start the high-pressure pump, and let the supercritical carbon dioxide flow into the extractor for extraction. After the extraction is completed, the supercritical fluid containing dissolved components is reduced to below the critical point, so that the dissolved effective components are separated out. The collected extract can be concentrated by rotary evaporation or the like, and the remaining carbon dioxide is removed to obtain a concentrated solution;

[0064] Enzymolysis: Pour room temperature ionized water into the pearl powder and mix and stir, adjust the pH value to 5, add compound enzyme at 35°C for enzymolysis, heat to inactivate the enzyme after the enzymolysis is completed, separate the liquid part and the solid residue, mix the liquid part with product A and stir, add acetic acid after stirring to adjust the pH value to 5, add compound enzyme at 35°C for enzymolysis, inactivate the enzyme by low temperature treatment after the enzymolysis is completed, separate and obtain a mixed extract, the compound enzyme is cellulase;

[0065] Preparation of wound plaster: Mix the mixed solution, concentrated solution, mixed extract and modified silver sulfadiazine at 0°C, make a paste after stirring, and store in a refrigerator to obtain a wound plaster.

[0066] The preparation method of modified silver sulfadiazine comprises:

[0067] S1. Place sulfadiazine in an ethanol solution and stir until completely dissolved. The ratio of sulfadiazine to 70% ethanol is 1 g:10 ml.

[0068] S2, dissolving silver nitrate in distilled water to form a silver nitrate solution;

[0069] S3, slowly adding the silver nitrate solution to the ethanol solution of sulfadiazine, during the mixing process, the pH value is controlled within 7, at a temperature of 1 ° C, slowly adding the modified dandelion extract and the modified aloe extract to the mixed solution after the mixing is completed, stirring, adding citric acid to ensure that the pH value is maintained within 3 during the mixing process, the dropping speed is 1 / s, and after the addition is completed, the surface modifier is added and stirred;

[0070] S4. Use vacuum filtration or centrifuge to separate the precipitate from the reaction solution of the mixed solution obtained in the above S3, wash the precipitate with distilled water for multiple times to remove unreacted reagents and by-products, and obtain modified silver sulfadiazine; in the modified silver sulfadiazine, the mass ratio of sulfadiazine to silver nitrate is 1:1, the added amount of modified dandelion extract is 5% of the total mass of sulfadiazine and silver nitrate, the added amount of modified aloe extract is 3% of the total mass of sulfadiazine and silver nitrate, and the added amount of modified modifier is 1% of the total mass of sulfadiazine and silver nitrate.

[0071] The preparation method of the modified dandelion extract comprises:

[0072] Grind dandelion and tea leaves in a mass ratio of 3:1 until the powders are evenly mixed, add 70% ethanol solution and soak at room temperature, the mass ratio of dandelion and tea mixed powder to ethanol solution is 1:5, the soaking time is 35 hours, during which irregular stirring is required, heat and reflux for 1.5 hours, extract 3 times in total, cool, filter, and concentrate under reduced pressure to obtain a concentrated extract, add citric acid dropwise to the concentrated extract and stir until the pH value reaches 3, and the addition is completed to obtain a modified dandelion extract.

[0073] The preparation method of the modified aloe extract comprises:

[0074] Aloe vera and tea powder are mixed evenly in a mass ratio of 2:1, and a 70% ethanol solution is added for soaking at room temperature. The mass ratio of the mixed powder of aloe vera and tea to the ethanol solution is 1:5. The soaking time is 35 hours, during which irregular stirring is required. The mixture is heated under reflux for 1.5 hours, extracted 3 times in total, cooled, filtered, and concentrated under reduced pressure to obtain a concentrated extract. Citric acid is added dropwise to the concentrated extract and stirred until the pH value reaches 3 and the addition is completed to obtain a modified aloe vera extract.

[0075] Example 3

[0076] Prescription: including borneol, pearl powder, angelica, modified silver sulfadiazine, coptis, licorice, and bletilla striata; the mass ratio of borneol, pearl powder, angelica, coptis, licorice, and bletilla striata is 2:1:2:2:3:1; the added amount of modified silver sulfadiazine is 5-8% of the total mass of borneol, bletilla striata, dragon's blood, and coptis.

[0077] A preparation method for a wound plaster comprises the following steps: preparing a mixed solution: weighing borneol, bletilla striata and coptis root according to a proportion, grinding them into powder and mixing them evenly, adding a 60% ethanol solution for soaking, the soaking time is 40 hours, stirring is required at irregular intervals during the soaking, heating and refluxing for 2.5 hours under ultrasonic conditions, extracting for a total of 5 times, cooling, combining the extracts, putting the extracts into a rotary evaporator to separate ethanol, and heating and stirring the extracts after removing ethanol until a viscous mixed solution is obtained;

[0078] Preparation of concentrated solution: Weigh angelica and licorice according to the proportion, grind into powder and mix evenly, add into cold water and soak, the cold water should be 2cm above the medicine, let it stand for 48h, keep the cold water temperature unchanged during the standing process, after the standing is over, filter to obtain the filtrate and product A;

[0079] Turn on the high-pressure pump and the extractor, put the filtrate into the extractor, compress the carbon dioxide gas to 300 bar, start the high-pressure pump, and let the supercritical carbon dioxide flow into the extractor for extraction. After the extraction is completed, the supercritical fluid containing dissolved components is reduced to below the critical point, so that the dissolved effective components are precipitated. The collected extract can be concentrated by rotary evaporation or the like, and the remaining carbon dioxide is removed to obtain a concentrated solution;

[0080] Enzymolysis: Pour room temperature ionized water into the pearl powder and mix and stir, adjust the pH value to 6, add compound enzyme at 40°C for enzymolysis, heat to inactivate the enzyme after the enzymolysis is completed, separate the liquid part and the solid residue, mix the liquid part with product A and stir, add acetic acid after stirring to adjust the pH value to 6, add compound enzyme at 40°C for enzymolysis, inactivate the enzyme by low temperature treatment after the enzymolysis is completed, separate and obtain a mixed extract, the compound enzyme is cellulase;

[0081] Preparation of wound plaster: Mix the mixed solution, concentrated solution, mixed extract and modified silver sulfadiazine at a temperature of 1°C, make a paste after stirring, and store in a refrigerator to obtain a wound plaster.

[0082] The preparation method of modified silver sulfadiazine comprises:

[0083] S1. Place sulfadiazine in an ethanol solution and stir until completely dissolved. The ratio of sulfadiazine to 75% ethanol is 1.5 g:10 ml.

[0084] S2, dissolving silver nitrate in distilled water to form a silver nitrate solution;

[0085] S3, slowly adding the silver nitrate solution to the ethanol solution of sulfadiazine, during the mixing process, the pH value is controlled within 8, at a temperature of 1 ° C, slowly adding the modified dandelion extract and the modified aloe extract to the mixed solution after the mixing is completed, stirring, adding citric acid to ensure that the pH value is maintained within 3 during the mixing process, the dropping speed is 2 / s, and after the addition is completed, the surface modifier is added and stirred;

[0086] S4. Use vacuum filtration or centrifuge to separate the precipitate from the reaction solution of the mixed solution obtained in the above S3, wash the precipitate with distilled water for multiple times to remove unreacted reagents and by-products, and obtain modified silver sulfadiazine; in the modified silver sulfadiazine, the mass ratio of sulfadiazine to silver nitrate is 1:1, the added amount of modified dandelion extract is 4-7% of the total mass of sulfadiazine and silver nitrate, the added amount of modified aloe extract is 5% of the total mass of sulfadiazine and silver nitrate, and the added amount of modified modifier is 1-3% of the total mass of sulfadiazine and silver nitrate.

[0087] The preparation method of the modified dandelion extract comprises:

[0088] Grind dandelion and tea leaves in a mass ratio of 3:1 until the powders are evenly mixed, add 80% ethanol solution and soak at room temperature, the mass ratio of dandelion and tea mixed powder to ethanol solution is 1:5, the soaking time is 30-40 hours, during which time stirring is required, heat and reflux for 2.5 hours, extract 5 times in total, cool, filter, and concentrate under reduced pressure to obtain a concentrated extract, add citric acid dropwise to the concentrated extract and stir until the pH value reaches 3, and the addition is completed to obtain a modified dandelion extract.

[0089] The preparation method of the modified aloe extract comprises:

[0090] Aloe vera and tea powder are mixed evenly in a mass ratio of 2:1, and an ethanol solution with a concentration of 80% is added for soaking at room temperature. The mass ratio of the mixed powder of aloe vera and tea to the ethanol solution is 1:5. The soaking time is 40 hours, during which irregular stirring is required. The mixture is heated under reflux for 2.5 hours, extracted 5 times in total, cooled, filtered, and concentrated under reduced pressure to obtain a concentrated extract. Citric acid is added dropwise to the concentrated extract and stirred until the pH value reaches 3 and the addition is completed to obtain a modified aloe vera extract.

[0091] Comparative Example 1

[0092] Compared with Example 2, in the preparation method of modified silver sulfadiazine in Control Example 1, modified dandelion extract and modified aloe extract were not added, and the rest was the same as in Example 2.

[0093] Comparative Example 2

[0094] Compared with Example 2, in Control Example 2, Borneol and Coptis chinensis were not added, and the rest was the same as in Example 2.

[0095] Comparative Example 3

[0096] Compared with Example 2, in Control Example 3, citric acid was not added to the modified dandelion extract to adjust the pH value to 3, and citric acid was not added to adjust the pH value when mixed with modified silver sulfadiazine. The rest was consistent with Example 2.

[0097] Comparative Example 4

[0098] Compared with Example 2, in Control Example 4, citric acid was not added to the modified aloe extract to adjust the pH value to 3, and citric acid was not added to adjust the pH value when mixed with modified silver sulfadiazine. The rest was the same as in Example 2.

[0099] Comparative Example 5

[0100] Compared with Example 2, in Control Example 5, in the preparation method of modified silver sulfadiazine, no surface modifier was added, and the rest was consistent with Example 2.

[0101] Comparative Example 6

[0102] Compared with Example 2, in Control Example 2, pearl powder, liquorice and bletilla striata were not added, and the rest was the same as in Example 2.

[0103] The present invention has carried out a lot of experimental studies, and the following are the results of the experimental studies of the present invention:

[0104] 1. Burn recovery experiment

[0105] 1.1 Experimental subjects

[0106] Select female SD rats, weighing 180g-200g; Guangxi Bama miniature pigs, weighing 20kg.

[0107] 1.2 Model establishment

[0108] Preparation of rat burn and scald model: After 5 days of adaptive feeding, the rats were shaved with an animal razor on both sides of the spine of the back about 2×2 cm, and then anesthetized with 10% chloral hydrate. The depilated area was disinfected with 75% medical alcohol, and the limbs were tied with rubber bands and fixed on the fixing board in a prone position. The depilated area was burned with a laser to a second degree burn, and the wound was prepared for use.

[0109] 1.3 Administration of drugs and reagents

[0110] Blank control group: normal saline

[0111] The experimental group of the present invention: the wound plaster prepared by the preparation method of Example 2 was refrigerated and stored. Usage and dosage: clean the wound with physiological saline, apply the product or apply the product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0112] The first control group: refrigerate the wound plaster prepared by the preparation method of control example 1. Usage and dosage: clean the wound with normal saline, apply this product or apply this product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0113] The second control group: the wound plaster prepared by the preparation method of control example 2 was refrigerated and stored. Usage and dosage: clean the wound with normal saline, apply this product or apply this product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0114] The third control group: the wound plaster prepared by the preparation method of control example 3 was refrigerated and stored. Usage and dosage: clean the wound with normal saline, apply this product or apply this product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0115] The fourth control group: the wound plaster prepared by the preparation method of control example 4 was refrigerated and stored. Usage and dosage: clean the wound with normal saline, apply this product or apply this product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0116] The fifth control group: the wound plaster prepared by the preparation method of control example 5 was refrigerated and stored. Usage and dosage: clean the wound with normal saline, apply this product or apply this product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0117] Sixth control group: The wound plaster prepared by the preparation method of control example 5 was refrigerated. Usage and dosage: clean the wound with normal saline, apply this product or apply this product on sterile gauze, cover the wound, bandage with sterile gauze, and change the dressing once in the morning and evening every day.

[0118] 1.4 Setting up the experiment

[0119] Experiment on the repair effect of mouse scald skin: The mice with scald model were divided into 8 groups, 10 mice in each group. The specific medication was referred to 1.3 for medication and reagents, and the wound healing rate was observed after 1 day, 3 days, 5 days and 7 days of medication. The specific data are shown in Table 1;

[0120] Wound healing rate % = (current wound area) / initial wound area×100%.

[0121] Table 1

[0122]

[0123] It can be seen that the wound healing rate of each group of mice 7 days after scalding is: the experimental control group of the present invention > the fifth control group > the fourth control group > the third control group > the second control group > the first control group > the sixth control group > the blank control group.

[0124] Example 4

[0125] Weigh 200g of angelica and 400g of licorice according to the proportion, grind into powder and mix evenly, add into cold water, soak the medicine until the cold water covers 2cm, let stand for 40h, keep the cold water temperature unchanged during the standing process, after the standing is over, filter to obtain the filtrate and product A;

[0126] Turn on the high-pressure pump and the extractor, put the filtrate into the extractor, compress the carbon dioxide gas to 200 bar, start the high-pressure pump, and let the supercritical carbon dioxide flow into the extractor for extraction. After the extraction is completed, the supercritical fluid containing dissolved components is reduced to below the critical point, so that the dissolved effective components are separated out. The collected extract can be concentrated by rotary evaporation or the like, and the remaining carbon dioxide is removed to obtain a concentrated solution;

[0127] Enzymolysis: Pour room temperature ionized water into 200g of pearl powder and mix and stir. After adjusting the pH value to 5, add compound enzyme for enzymolysis at a temperature of 35°C. After the enzymolysis is completed, heat to inactivate the enzyme, separate the liquid part and the solid residue, mix the liquid part with product A, stir, add acetic acid dropwise after stirring to adjust the pH value to 5, add compound enzyme for enzymolysis at a temperature of 35°C. After the enzymolysis is completed, use low temperature treatment to inactivate the enzyme, and separate to obtain a mixed extract.

[0128] Comparative Example 6

[0129] Compared with Example 4, in Comparative Example 6, 200 g of Angelica sinensis and 400 g of Licorice were weighed in proportion, ground into powder and mixed evenly, then added into cold water, and the cold water was soaked to a level that the medicine was submerged by 2 cm, and allowed to stand for 40 hours. During the standing process, the cold water temperature was kept constant. After the standing was completed, the filtrate and Product A were obtained by filtration;

[0130] The filtrate is concentrated by rotary evaporation or the like to remove the remaining carbon dioxide to obtain a concentrated solution;

[0131] Enzymolysis: Pour room temperature ionized water and product A into 100g of pearl powder and mix and stir. After adjusting the pH value to 5, add complex enzyme for enzymolysis at a temperature of 35°C. After the enzymolysis is completed, heat to inactivate the enzyme, separate the liquid part and the solid residue, use low temperature treatment to inactivate the enzyme, and separate to obtain a mixed extract.

[0132] Comparative Example 7

[0133] Weigh 200g of angelica and 400g of licorice according to the proportion, grind into powder and mix evenly, add into cold water, soak the medicine until the cold water covers 2cm, and let it stand for 31h. During the standing process, keep the cold water temperature unchanged. After the standing is over, filter to obtain the filtrate and product A;

[0134] The filtrate is extracted, and after the extraction is completed, the collected extract is concentrated by rotary evaporation or the like to remove the remaining carbon dioxide to obtain a concentrated solution;

[0135] Enzymolysis: Pour room temperature ionized water into 200g of pearl powder and mix and stir. After adjusting the pH value to 5, add compound enzyme for enzymolysis at a temperature of 35°C. After the enzymolysis is completed, heat to inactivate the enzyme, separate the liquid part and the solid residue, mix the liquid part with product A, stir, add compound enzyme for enzymolysis at a temperature of 30°C, after the enzymolysis is completed, use low temperature treatment to inactivate the enzyme, and separate to obtain a mixed extract.

[0136] The concentrated solution of Example 5 and the mixed extract were mixed to calculate the extraction rate, the concentrated solution of Reference Example 6 and the mixed extract were mixed to calculate the extraction rate, and the concentrated solution of Reference Example 7 and the mixed extract were mixed to calculate the extraction rate. The specific extraction rate data are shown in Table 2;

[0137] Extraction rate (%) = (mass of extract / mass of original Chinese medicinal material) × 100%

[0138] Table 2

[0139] Example 4 Comparative Example 6 Comparative Example 7 Extraction rate % 22.3％ 14.8％ 18.9％

[0140] It can be seen that the extraction rate of Example 4 is greater than that of Control Example 6. By using a multi-stage extraction process, we can ensure that effective ingredients are extracted from various parts of the medicinal materials, achieve full component extraction, and comprehensively extract a variety of active ingredients in traditional Chinese medicine to ensure that no important pharmacological substances are missed. In the preparation of the concentrated solution, the heat-sensitive components in the traditional Chinese medicine are first extracted by cold immersion. Similarly, the pearl powder is first enzymatically hydrolyzed to extract most of the components, and the remaining filter residue and the remaining A product are subjected to secondary extraction by enzymatic hydrolysis to improve the extraction rate and purity.

[0141] 2. Clinical observation

[0142] 2.1 Experimental subjects: From 2022 to date, the inventor has conducted clinical observation and treatment on 320 burn patients, including 180 males and 140 females, aged 17 to 58 years old.

[0143] 2.2 Treatment method: After cleaning the affected area, apply the ointment of the present invention 2 to 3 times a day, calculated according to the severity of the patient's condition.

[0144] The treatment time is about 4 to 6 days for patients with mild burns, and 10 to 17 days for patients with second-degree burns.

[0145] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the patent and protection scope of the present invention shall be subject to the attached claims.

Claims

1. A wound plaster, characterized in that: The invention comprises borneol, pearl powder, angelica, modified silver sulfadiazine, coptis root, liquorice and bletilla striata, wherein the mass ratio of borneol, pearl powder, angelica, coptis root, liquorice and bletilla striata is 1-2:0.5-1:1-2:1-2:2-3:0.5-1, and the added amount of modified silver sulfadiazine is 5-8% of the total mass of borneol, bletilla striata, dragon's blood and coptis root.

2. A method for preparing a wound plaster as claimed in claim 1, characterized in that: The following steps are involved: Preparation of mixed solution: Weigh borneol, Bletilla striata and Coptis chinensis according to the proportion, grind into powder and mix evenly, add 50-60% ethanol solution for soaking, soaking time is 30-40 hours, stirring is required at irregular intervals during the soaking, and then heat and reflux for 1.5-2.5 hours under ultrasonic conditions, extract 3-5 times in total, cool, combine the extracts, put the extracts into a rotary evaporator to separate ethanol, and heat and stir the extracts after removing ethanol until it becomes viscous to obtain a mixed solution; Preparation of concentrated solution: Weigh angelica and licorice according to the proportion, grind into powder and mix evenly, add into cold water and soak, let stand for 35-48 hours, keep the cold water temperature unchanged during the standing process, after the standing is completed, filter to obtain the filtrate and product A; Turn on the high-pressure pump and the extractor, preheat to 30-60°C, put the filtrate into the extractor, compress the carbon dioxide gas to 100-300 bar, start the high-pressure pump, and let the supercritical carbon dioxide flow into the extractor for extraction. After the extraction is completed, the supercritical fluid containing dissolved components is reduced to below the critical point, so that the dissolved effective components are separated out. The collected extract can be concentrated by rotary evaporation or the like, and the remaining carbon dioxide is removed to obtain a concentrated solution; Enzymolysis: Pour room temperature ionized water into the pearl powder and mix and stir, adjust the pH value to 5-6, add compound enzyme for enzymolysis at 30-40℃, heat to inactivate the enzyme after the enzymolysis is completed, separate the liquid part and the solid residue, mix the liquid part with product A and stir, add acetic acid to adjust the pH value to 5-6 after stirring, add compound enzyme for enzymolysis at 30-40℃, after the enzymolysis is completed, use low temperature treatment to inactivate the enzyme, separate and obtain the mixed extract; Preparation of wound plaster: Mix the mixed solution, concentrated solution, mixed extract and modified silver sulfadiazine at a temperature of 0-1°C, make a paste after stirring, and store in a refrigerator to obtain a wound plaster.

3. A method for preparing a wound plaster as claimed in claim 2, characterized in that: The preparation method of modified silver sulfadiazine comprises: S1. Place sulfadiazine in an ethanol solution and stir until completely dissolved. The ratio of sulfadiazine to 65-75% ethanol is 0.5-1.5 g:10 ml. S2, dissolving silver nitrate in distilled water to form a silver nitrate solution; S3, slowly adding the silver nitrate solution to the ethanol solution of sulfadiazine, and during the mixing process, the pH value is controlled within 6-8; S4. Using vacuum filtration or a centrifuge to separate the precipitate from the reaction solution of the mixed solution obtained in S3, and washing the precipitate with distilled water for multiple times to remove unreacted reagents and by-products, thereby obtaining modified silver sulfadiazine.

4. A method for preparing a wound plaster as claimed in claim 3, characterized in that: In the preparation method S3 of modified silver sulfadiazine, at a temperature of 0-1°C, the modified dandelion extract and the modified aloe extract are slowly added to the mixed mixture with stirring, and the dropping speed is 1-2 / s. After the addition is completed, the surface modifier is added with stirring. After the stirring is completed, the precipitate is separated from the reaction solution by vacuum filtration or centrifuge, and the precipitate is washed with distilled water for multiple times to remove unreacted reagents and by-products to obtain modified silver sulfadiazine, which is the final product.

5. A method for preparing a wound plaster as claimed in claim 4, characterized in that: The preparation method of the modified dandelion extract comprises: Grind dandelion and tea leaves into powders according to a certain proportion and mix them evenly; add 60-80% ethanol solution and soak them at room temperature; the mass ratio of the mixed powder of dandelion and tea leaves to the ethanol solution is 1:5; the soaking time is 30-40 hours; during this period, stirring is required from time to time; heat and reflux for 1.5-2.5 hours; extract 3-5 times in total; cool, filter, and concentrate under reduced pressure to obtain a concentrated extract; add citric acid dropwise to the concentrated extract and stir until the pH value reaches 3 and the addition is completed to obtain a modified dandelion extract.

6. A method for preparing a wound plaster as claimed in claim 4, characterized in that: The preparation method of the modified aloe extract comprises: Aloe vera and tea powder are mixed evenly in proportion, and an ethanol solution with a concentration of 60-80% is added for soaking at room temperature. The mass ratio of the mixed powder of aloe vera and tea to the ethanol solution is 1:

5. The soaking time is 30-40 hours, during which irregular stirring is required. The mixture is heated under reflux for 1.5-2.5 hours, and extracted 3-5 times in total. The mixture is cooled, filtered, and concentrated under reduced pressure to obtain a concentrated extract. Citric acid is added dropwise to the concentrated extract and stirred until the pH value reaches 3 and the addition is completed to obtain a modified aloe vera extract.

7. A method for preparing a wound plaster as claimed in claim 2, characterized in that: In the enzymatic hydrolysis step, the complex enzyme is one or more of xylanase, cellulase, protease and hemicellulase.

8. A method for preparing a wound plaster as claimed in claim 4, characterized in that: In the modified silver sulfadiazine, the mass ratio of sulfadiazine to silver nitrate is 1:1, the added amount of the modified dandelion extract is 4-7% of the total mass of sulfadiazine and silver nitrate, the added amount of the modified aloe extract is 2-5% of the total mass of sulfadiazine and silver nitrate, and the added amount of the modified modifier is 1-3% of the total mass of sulfadiazine and silver nitrate.

9. A method for preparing a wound plaster as claimed in claim 5, characterized in that: In the preparation method of the modified dandelion extract, the mass ratio of dandelion to tea is 2:

1.

10. A method for preparing a wound plaster as claimed in claim 6, characterized in that: The mass ratio of aloe vera and tea powder is 3:1.

Images