Auxiliary device for bovine tuberculosis kit detection and use method thereof

Abstract

The invention belongs to the technical field of biological detection, and particularly relates to an auxiliary device for bovine tuberculosis kit detection and a use method of the auxiliary device. The problems that existing bovine tuberculosis detection equipment is large in size, inconvenient to carry to the site, complex in operation, low in detection efficiency and high in detection cost are solved. According to the scheme, a plurality of vibration assemblies are arranged on a box body, the vibration assemblies are connected with a supporting assembly, the vibration assemblies are used for driving the supporting assembly to move up and down, a storage box is placed on the supporting assembly, a pore plate is arranged on the storage box, and a sample tube is placed on the pore plate; the auxiliary device for bovine tuberculosis kit detection has the advantages of being small in size, light in weight, easy and convenient to operate, convenient to carry, low in cost and the like so as to meet the requirement for on-site rapid detection, especially in areas with limited resources, the timeliness and accuracy of bovine tuberculosis diagnosis are improved, prevention and control measures can be taken in time, and the detection efficiency is improved. Disease transmission risks are reduced, and healthy development of animal husbandry is guaranteed.

Description

Technical Field

[0001] The invention relates to an auxiliary device for bovine tuberculosis test kit detection and a use method thereof, belonging to the technical field of biological detection. Background Art

[0002] Bovine tuberculosis is a chronic infectious disease that seriously threatens the healthy development of animal husbandry. Its early and accurate diagnosis is of great significance for controlling the spread of the epidemic, ensuring the health of cattle and food safety. At present, the diagnosis of bovine tuberculosis mainly relies on laboratory testing, especially the kit detection method based on specific antigen-antibody reaction, which is widely used due to its high sensitivity and strong specificity. However, in actual operation, the traditional kit detection process has many inconveniences and challenges.

[0003] In the traditional testing process, in order to ensure that the reaction solution in the test kit is fully mixed, it is usually necessary to rely on professional laboratory equipment or manual shaking to mix the sample and reagent. This not only requires the operator to have certain professional skills, but also should avoid the deviation of the test results caused by uneven shaking. In addition, the traditional equipment is bulky and inconvenient to carry to veterinary stations or farms in remote areas for on-site testing, which limits the efficiency and coverage of the test. In addition, some remote areas lack sufficient laboratory facilities to support the operation and maintenance of multiple devices, which further increases the difficulty of rapid diagnosis of bovine tuberculosis.

[0004] In summary, an auxiliary device that can overcome the above defects is needed, which can not only realize uniform and rapid mixing of the liquid in the test kit, but also has the characteristics of small size, light weight, simple operation, and easy to carry, so as to meet the needs of rapid on-site detection and improve the timeliness and accuracy of bovine tuberculosis diagnosis, especially in areas with limited resources. Therefore, the present invention proposes a portable auxiliary device for bovine tuberculosis test kit detection, which aims to solve the deficiencies of traditional equipment in portability, operation complexity and detection efficiency through innovative design, and provide a more efficient and convenient detection means for the prevention and control of bovine tuberculosis, which helps to take prevention and control measures in time, reduce the risk of disease transmission, and ensure the healthy development of animal husbandry. Summary of the invention

[0005] The present invention provides an auxiliary device for bovine tuberculosis test kit detection and a method for using the same to solve the problems that existing bovine tuberculosis detection equipment is bulky, inconvenient to carry to the site, complicated to operate, low in detection efficiency and high in detection cost.

[0006] The present invention achieves the above-mentioned object through the following technical scheme: an auxiliary device for bovine tuberculosis test kit detection, comprising a box body, on which a plurality of vibration components are arranged, and a plurality of the vibration components are connected to support components, and the vibration components are used to drive the support components to shake; The support assembly is provided with a storage box for supporting the storage box; The storage box is provided with a well plate, and the well plate is provided with a plurality of culture wells; The culture well is used for placing a sample tube.

[0007] As a further solution of the present invention: the sample tube is cylindrical, the upper end of the sample tube is open, the bottom end is sealed, and the sample tube is adapted to the culture well; A baffle is arranged on the inner wall of the sample tube. The baffle is narrow at both ends and wide in the middle, and is arranged on the inner wall of the sample tube in a crescent shape. The baffle divides the sample tube into upper and lower layers. A through hole is formed in the center of the baffle to penetrate the upper and lower layers of the sample tube, and the narrow sides at both ends of the baffle are not connected.

[0008] As a further solution of the present invention: a plurality of outer tube clamps are correspondingly arranged on the outer wall of the sample tube, and a plurality of inner tube clamps are correspondingly arranged on the inner wall of the culture hole, and the outer tube clamps and the inner tube clamps are both L-shaped and fit each other.

[0009] As a further solution of the present invention: the vibration assembly includes a plurality of vibration tubes, the vibration tubes are arranged in a tubular shape at the corners of the box body, each of the vibration tubes is sleeved with a power rod and a spring, and a groove is provided on one side of the vibration tube close to the support assembly; One end of the power rod is fixedly connected to the top of the inner wall of the box body, and the other end is in contact with the support assembly. The power rod is used to control the downward movement of the support assembly; One end of the spring is connected to the bottom of the box body, and the other end is connected to the support assembly. The spring is used to control the support assembly to reset upward.

[0010] As a further solution of the present invention: the support assembly includes: an inner frame, a support frame, and a ball head; the support frame is fixedly connected to the inner frame, the inner frame is adapted to the storage box, a ball head is provided at one end of the support frame close to the vibration assembly, the ball head is spherical and clamped in the vibration tube, the support frame passes through the groove of the vibration tube, and the ball head moves up and down in the vibration tube.

[0011] As a further solution of the present invention: a stabilizing component is provided between the storage box and the inner frame, and the stabilizing component is used to stabilize the storage box.

[0012] As a further solution of the present invention: a plurality of light-emitting diodes are arranged in the storage box, and the plurality of light-emitting diodes correspond one-to-one to the culture wells, and the corresponding light-emitting diodes are located on the bottom plate of the storage box directly below the center of the culture well; a photosensitive switch is arranged on the inner wall of each culture well, and the corresponding photosensitive switch is electrically connected to the light-emitting diode.

[0013] As a further solution of the present invention: the top of the box body is provided with an opening, and a transparent top cover is provided on the opening.

[0014] As a further solution of the present invention: a temperature display panel is arranged on the box body, and a constant temperature component is arranged on the inner wall of the inner frame body.

[0015] The present invention also provides a method for using an auxiliary device for bovine tuberculosis test kit detection, comprising the following steps: S1, sample preparation: fix the storage box in the inner frame, prepare sterile and clean sample tubes; aseptically collect whole blood from cattle and put it into the blood collection tube containing anticoagulant blood, gently turn it upside down 5 to 10 times to fully mix the blood sample, and then add each portion of anticoagulant blood into the sample tube; S2, put the sample tube into the culture well, rotate the sample tube counterclockwise, and the outer tube clamp and the inner tube clamp cooperate and clamp tightly, so that the sample tube and the well plate are relatively locked, and then turn on the photosensitive switch and the light-emitting diode; S3, add solution, tilt and rotate the sample tube so that the wide part of the baffle plate faces upward, and the smooth inner wall of the sample tube penetrates the baffle plate from top to bottom; the sample tube can be observed from above the culture hole, and the on and off status of the light-emitting diode can be used to quickly determine whether the blood sample solution is added to the current sample tube, so as to prevent omission, wrong addition, repeated addition, etc. S4, sample mixing, a number of power rods are activated in sequence to perform telescopic movements, when the power rods are extended, the ball heads are driven to move downward in sequence, when the power rods are contracted, the springs push the ball heads to return upward; the ball heads drive the support frame to move up and down in sequence, and then drive the inner frame to shake in the box, so that the blood sample in the sample tube is fully mixed; S5, blood culture, after the blood sample is fully mixed, the constant temperature component is turned on, so that the temperature in the auxiliary device of the bovine tuberculosis test kit is set to 37° C. and cultured for 16-20 hours to obtain the blood culture supernatant; S6, take out the sample, take out the orifice plate from the inner frame, and then rotate the sample tube clockwise to separate the outer tube clamp from the inner tube clamp, and lift the sample tube to remove it from the orifice plate; S7, supernatant separation, tilt and rotate the sample tube so that the wide part of the baffle of the sample tube is facing downward, and use a pipette or other extraction equipment to extract the blood sample. The supernatant is blocked by the baffle in the upper layer of the baffle and does not mix with the lower layer sample; the mixing of the blood sample and the supernatant can be effectively avoided, thereby improving the detection accuracy and efficiency.

[0016] The beneficial effects of the present invention are: 1. In the present invention, a plurality of vibration components are arranged on the box body, and the plurality of vibration components are connected with support components, and the vibration components are used to drive the support components to move up and down, and the support components support the orifice plate, and the orifice plate can be used to place the sample tube; the present invention has the advantages of small size, light weight, simple operation, easy to carry, low cost, etc.; to meet the needs of rapid on-site detection, especially in areas with limited resources, to improve the timeliness and accuracy of bovine tuberculosis diagnosis, to provide a more efficient and convenient detection means for the prevention and control of bovine tuberculosis, to help to take prevention and control measures in time, to reduce the risk of disease transmission, and to ensure the healthy development of animal husbandry.

[0017] 2. In the present invention, the power rod performs telescopic movement in sequence, and when the power rod is extended, the ball head is driven to move downward in sequence. When the power rod is contracted in sequence, the spring pushes the ball head to reset upward in sequence, and the ball head drives the support frame to move up and down in sequence, thereby driving the inner frame to swing in the box, so that the sample in the sample tube can be fully mixed, and the deviation of the test result caused by uneven artificial shaking can be avoided; an opening is arranged on the top of the box, and a transparent top cover is movably arranged on the opening, and the top cover is used for heat preservation and preventing impurities from falling into the sample tube of the storage box during shaking and blood culture of the auxiliary device for the detection of bovine tuberculosis test kit, and at the same time, it is convenient for the staff to observe the situation in time and prevent accidents; a temperature display panel is arranged on the box, and the temperature display panel can timely feedback the temperature in the inner frame, which is convenient for timely understanding of the environment during blood culture and sample storage; a constant temperature component is arranged on the inner wall of the inner frame, and the constant temperature component can realize accurate control of the temperature in the auxiliary device for the detection of bovine tuberculosis test kit through the cooperation of the temperature sensor and the circuit board.

[0018] 3. In the present invention, the baffle is narrow at both ends and wide in the middle, and is arranged on the inner wall of the sample tube in a crescent shape. When the sample and the supernatant are separated, the sample tube is tilted and rotated so that the wide body part of the baffle of the sample tube is facing downward. At this time, the sample is extracted using an extraction device such as a pipette, and the supernatant is blocked on the baffle by the baffle and does not mix with the lower layer sample, which can effectively avoid the mixing of the sample and the supernatant, thereby improving the detection accuracy and efficiency; the narrow sides at both ends of the baffle are not connected, and the center is formed. The through hole runs through the upper and lower layers of the sample tube, which can facilitate the addition of solution into the sample tube or facilitate the smooth pouring of the sample solution in the lower layer.

[0019] 4. In the present invention, a plurality of outer tube clamps are correspondingly arranged on the outer wall of the sample tube, and a plurality of inner tube clamps are correspondingly arranged on the inner wall of the culture hole. The outer tube clamps and the inner tube clamps are both L-shaped and fit each other. The outer tube clamps and the inner tube clamps are arranged to lock the sample tube to prevent the sample tube from rotating in the culture hole, which is beneficial to stabilizing the sample tube on the hole plate so that the sample tube and the hole plate are relatively locked.

[0020] 5. In the present invention, a plurality of light-emitting diodes are arranged in the well plate, and the plurality of light-emitting diodes correspond to the culture wells one by one. A photosensitive switch is arranged on the inner wall of each culture well, and the corresponding photosensitive switch is electrically connected to the light-emitting diode. When the light of the photosensitive switch is blocked by the blood sample, the corresponding photosensitive switch is turned off, so that the corresponding light-emitting diode emits light. When the blood sample is drawn out from the sample tube, the light-emitting diode will not be turned off. The staff can quickly distinguish whether the blood sample is added to the current sample tube according to the on and off status of the light-emitting diode, which can effectively avoid omission, wrong addition, repeated addition, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic cross-sectional view of the overall structure of the present invention; Figure 3 This is a schematic diagram of the overall structure of the storage box of the present invention; Figure 4 It is a schematic diagram of the cross-sectional structure of the storage box of the present invention; Figure 5 For the present invention Figure 4 A schematic diagram of the structure enlargement in the middle; Figure 6 This is a schematic diagram of the sample tube structure of the present invention; Figure 7 It is a schematic diagram of the cross-sectional structure of the sample tube of the present invention.

[0022] In the figure: 1. Box body; 11. Temperature display panel; 2. Vibration assembly; 21. Vibration tube; 22. Power rod; 23. Spring; 3. Support assembly; 31. Inner frame; 32. Support frame; 33. Ball head; 4. Storage box; 41. Well plate; 42. Culture well; 43. Inner tube clamp; 44. Light-emitting diode; 5. Sample tube; 51. Baffle; 52. Outer tube clamp; 6. Stabilizing assembly. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Embodiment 1 like Figures 1 to 4 As shown, an auxiliary device for bovine tuberculosis test kit detection includes a box body 1, a plurality of vibration components 2 are arranged on the box body 1, and the plurality of vibration components 2 are connected to support components 3, and the vibration components 2 are used to drive the support components 3 to move up and down; The vibration assembly 2 includes a plurality of vibration tubes 21, which are arranged in a tubular shape at the corners of the box body 1. A power rod 22 and a spring 23 are sleeved in each vibration tube 21. A groove is provided on one side of the vibration tube 21 close to the support frame 32, and the groove matches with the support frame 32. The upper end of the power rod 22 is fixedly connected to the top of the inner wall of the box body 1, and the lower end is in contact with the ball head 33. The power rod 22 can be a telescopic device such as a telescopic motor or a hydraulic rod. The power rod 22 is used to control the downward movement of the ball head 33; One end of the spring 23 is connected to the bottom of the box 1, and the other end is connected to the ball head 33. The spring 23 is used to control the ball head 33 to reset upward; The support assembly 3 includes an inner frame 31, a support frame 32, and a ball head 33; The inner frame 31 is fixedly connected with a support frame 32, and a ball head 33 is provided at one end of the support frame 32 close to the vibration tube 21, and the inner frame 31 is adapted to the storage box 4; the support frame 32 passes through the groove of the vibration tube 21 and moves up and down in the groove; The ball head 33 is spherically clamped in the vibration tube 21, and the support frame 32 drives the ball head 33 to move up and down in the vibration tube 21; when the power rod 22 is extended, the ball head 33 moves downward, and the spring 23 retracts downward; when the power rod 22 retracts, the ball head 33 moves upward, and the spring 23 returns upward; the spherical setting of the ball head 33 can ensure that when multiple ball heads 33 move up and down in multiple vibration tubes 21, the ball heads 33 that are not in the same horizontal plane will not get stuck with the vibration tube 21; The device further comprises a storage box 4, on which a well plate 41 is disposed. Usually, the well plate 41 has 24 wells, 48 ​​wells or 96 wells, etc.; the well plate 41 is provided with a plurality of culture wells 42, and the culture wells 42 are used to place the sample tubes 5; The sample tube 5 is cylindrical, with an open top and a sealed bottom. The diameter of the sample tube 5 matches the diameter of the culture well 42 and can be placed in the culture well 42. The sample tube 5 is used to store the sample solution. Usually, the specifications of the sample tube 5 are 0.5 ml, 1 ml, 1.5 ml, 2 ml, etc. like Figure 6 , Figure 7As shown, a baffle 51 is arranged on the inner wall of the sample tube 5. The baffle 51 is used to prevent the supernatant from mixing with the sample after the sample and the supernatant are separated. The baffle 51 is narrow at both ends and wide in the middle. It is arranged on the inner wall of the sample tube 5 in a crescent shape. A through hole is formed in the center of the baffle 51. The baffle 51 divides the sample tube 5 into upper and lower layers that are interconnected. The through hole formed in the center of the baffle 51 penetrates the upper and lower layers of the sample tube 5. The height of the baffle 51 is set in multiple models according to the sample requirements, including 0.5ml, 1m l, 1.5ml, etc.; for example, if 1ml of sample needs to be extracted, a 1.5ml sample tube 5 is used, and the volume of the lower layer of the baffle 51 is 1ml. After the sample and the supernatant are separated, the sample tube 5 is tilted and rotated so that the wide part of the baffle 51 of the sample tube 5 is facing downward. At this time, the sample is extracted using an extraction device such as a pipette, and the supernatant is blocked on the baffle 51 and does not mix with the lower layer sample, which can effectively prevent the sample from mixing with the supernatant, thereby improving the detection accuracy and efficiency; The narrow sides of the two ends of the baffle 51 are not connected, and the inner wall of the sample tube 5 is smooth and runs through the baffle 51 from top to bottom; when other solutions need to be added, the sample tube 5 is tilted and rotated so that the wide part of the baffle 51 faces upward, which makes it convenient to add solutions to the sample tube 5 or to pour out the sample solution at the lower layer smoothly.

[0025] When the blood sample needs to be fully mixed, several power rods 22 are started in sequence to perform telescopic movements. When the power rods 22 are extended, the ball heads 33 are driven to move downward in sequence. When the power rods 22 are contracted in sequence, the springs 23 push the ball heads 33 to return upward in sequence. When one of the power rods 22 is extended, the power rods 22 on the diagonal line are contracted, and the cycle repeats; the ball heads 33 drive the corresponding support frames 32 to move up and down in sequence, and then drive the inner frame 31 to swing in the box body 1, so that the sample in the sample tube 5 is fully mixed, avoiding uneven artificial shaking that may cause deviation in the test results.

[0026] Compared with the prior art, the auxiliary device for bovine tuberculosis test kit detection disclosed in the embodiment of the present invention has the advantages of small size, light weight, simple operation, easy to carry, low cost, etc.; it can meet the needs of rapid on-site detection, especially in areas with limited resources, improve the timeliness and accuracy of bovine tuberculosis diagnosis, and provide a more efficient and convenient detection method for the prevention and control of bovine tuberculosis, which is helpful to take prevention and control measures in time, reduce the risk of disease transmission, and ensure the healthy development of animal husbandry.

[0027] Embodiment 2 Improvements based on Example 1: like Figures 5 to 7As shown, a plurality of outer tube clamps 52 are correspondingly arranged on the outer wall of the sample tube 5, and a plurality of inner tube clamps 43 are correspondingly arranged on the inner wall of the culture hole 42. The outer tube clamps 52 and the inner tube clamps 43 are both L-shaped, and the outer tube clamps 52 and the inner tube clamps 43 are adapted to each other to lock the sample tube 5 to prevent the sample tube 5 from rotating in the culture hole 42. When the sample tube 5 needs to be fixed on the orifice plate 41, the sample tube 5 is inserted into the culture hole 42, and the sample tube 5 is rotated counterclockwise so that the outer tube clamps 52 and the inner tube clamps 43 cooperate. At this time, the sample tube 5 is fixed relative to the orifice plate 41. When the sample tube 5 needs to be removed from the orifice plate 41, the sample tube 5 is rotated clockwise so that the outer tube clamps 52 and the inner tube clamps 43 are separated. At this time, the sample tube 5 can be lifted to remove the sample tube 5 from the culture hole 42. The arrangement of the outer tube clamps 52 and the inner tube clamps 43 is conducive to stabilizing the sample tube 5 on the orifice plate 41, so that the sample tube 5 and the orifice plate 41 are relatively locked.

[0028] Further, such as Figure 1 , Figure 2 As shown, a stabilizing component 6 is provided between the storage box 4 and the inner frame 31, and the stabilizing component 6 includes an inverted V-shaped spring sheet, and a plurality of inverted V-shaped spring sheets are provided around the inner wall of the inner frame 31. When the storage box 4 is placed in the inner frame 31, the opening of the spring sheet contracts and squeezes the storage box 4, so that the storage box 4 will not move randomly in the inner frame 31 when the bovine tuberculosis test kit detection auxiliary device moves. When the storage box 4 needs to be taken out, the inverted V-shaped spring sheet on one side continues to be compressed so that the spring sheet on the opposite side is separated from the storage box 4; Furthermore, the stabilizing component 6 may also be a component with shrinkage properties such as a foam sponge; the stabilizing component 6 is used to stabilize the storage box 4 and make it more convenient to take out the storage box 4.

[0029] Further, such as Figures 2 to 4 As shown, a plurality of light emitting diodes 44 are arranged in the storage box 4, and the plurality of light emitting diodes 44 correspond to the culture holes 42 one by one, and the corresponding light emitting diodes 44 are located on the bottom plate of the storage box 4 just below the center of the culture hole 42; A photosensitive switch is correspondingly arranged on the inner wall of each culture hole 42, and the photosensitive switch is electrically connected to the light-emitting diode 44. The photosensitive switch is used to sense the addition of the sample solution in the sample tube 5 and control the on and off of the light-emitting diode 44; The staff puts the sample tube 5 into the culture hole 42, and then adds the blood sample into the sample tube 5. The light of the photosensitizer is blocked by the blood sample, and the corresponding photosensitizer is turned off, causing the corresponding light-emitting diode 44 to emit light. When the blood sample is drawn out of the sample tube 5, the light-emitting diode 44 will not be turned off. The staff can quickly determine whether the blood sample is currently added to the sample tube 5 based on the on and off status of the light-emitting diode 44, which can effectively avoid omissions, wrong additions, repeated additions, etc.

[0030] Further, such as Figure 1 As shown, an opening is provided on the top of the box 1, the shape of the opening is consistent with the shape of the storage box 4 and the size of the opening is larger than the size of the storage box 4, and the opening is used to take out the storage box 4; an acrylic transparent top cover is movably provided on the opening, and the top cover is used to keep warm and prevent impurities from falling into the sample tube 5 of the storage box 4 during shaking of the bovine tuberculosis test kit detection auxiliary device and blood culture, and at the same time, it is convenient for the staff to observe the situation in time to prevent accidents.

[0031] Furthermore, a temperature display panel 11 is provided on the outer wall of the box body 1, and the temperature display panel 11 can timely feedback the temperature inside the inner frame body 31, so as to facilitate timely understanding of the environment during blood culture and sample storage; a constant temperature component is provided on the inner wall of the inner frame body 31, and the constant temperature component can realize precise control of the temperature inside the auxiliary device of the bovine tuberculosis test kit through the cooperation of the temperature sensor and the circuit board.

[0032] The present invention also provides a method for using an auxiliary device for bovine tuberculosis test kit detection, comprising the following steps: S1, sample preparation: fix the storage box 4 in the inner frame 31, prepare a sterile and clean sample tube 5; aseptically collect whole blood from cattle and put it into a blood collection tube containing anticoagulant blood, gently turn it upside down 5 to 10 times to fully mix the blood sample, and then add each portion of anticoagulant blood into the sample tube 5; S2, put the sample tube 5 into the culture well 42, rotate the sample tube 5 counterclockwise, the outer tube clamp 52 and the inner tube clamp 43 cooperate and clamp, so that the sample tube 5 and the well plate 41 are relatively locked, and then turn on the photosensitive switch and the light-emitting diode 44; S3, add solution, tilt and rotate the sample tube 5 so that the wide part of the baffle 51 faces upward, and the inner wall of the sample tube 5 is smooth and penetrates the baffle 51 from top to bottom; the sample tube 5 can be observed from above the culture hole 42, and it can be quickly determined whether the blood sample solution is added to the current sample tube 5 according to the on and off status of the light-emitting diode 44, so as to prevent omission, wrong addition, repeated addition, etc. S4, sample mixing, a plurality of power rods 22 are sequentially activated to perform telescopic movements, when the power rods 22 are extended, the ball heads 33 are sequentially driven to move downward, when the power rods 22 are contracted, the springs 23 push the ball heads 33 to return upward; the ball heads 33 drive the support frame 32 to move up and down in sequence, and then drive the inner frame 31 to shake in the box body 1, so that the blood sample in the sample tube 5 is fully mixed; S5, blood culture, after the blood sample is fully mixed, the constant temperature component is turned on, so that the temperature in the auxiliary device of the bovine tuberculosis test kit is set to 37° C. and cultured for 16-20 hours to obtain the blood culture supernatant; S6, take out the sample, take out the orifice plate 41 from the inner frame 31, then rotate the sample tube 5 clockwise to separate the outer tube clamp 52 from the inner tube clamp 43, and lift the sample tube 5 to remove it from the orifice plate 41; S7, supernatant separation, tilt and rotate the sample tube 5 so that the wide part of the baffle plate 51 of the sample tube 5 is facing downward, and use a pipette or other extraction equipment to extract the blood sample. The supernatant is blocked by the baffle plate 51 in the upper layer of the baffle plate 51 and is not mixed with the lower layer sample; the mixing of the blood sample and the supernatant can be effectively avoided, thereby improving the detection accuracy and efficiency.

[0033] However, the working principles and wiring methods of the light-emitting diode 44, temperature display panel 11, constant temperature component and photosensitive switch are commonplace and are conventional means or common knowledge, and will not be elaborated here. Technicians in the field can make any selections according to their needs or convenience.

[0034] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0035] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An auxiliary device for bovine tuberculosis test kit detection, comprising a box (1), characterized in that: A plurality of vibration components (2) are arranged on the box body (1), and a plurality of the vibration components (2) are connected to support components (3), and the vibration components (2) are used to drive the support components (3) to shake; The support assembly (3) is provided with a storage box (4), and the support assembly (3) is used to hold up the storage box (4); The storage box (4) is provided with a well plate (41), and the well plate (41) is provided with a plurality of culture wells (42); The culture hole (42) is used to place the sample tube (5).

2. An auxiliary device for bovine tuberculosis test kit detection according to claim 1, characterized in that: The sample tube (5) is cylindrical, the upper end of the sample tube (5) is open, the lower end is sealed, and the sample tube (5) is compatible with the culture hole (42); A baffle (51) is arranged on the inner wall of the sample tube (5); the baffle (51) is narrow at both ends and wide in the middle, and is arranged on the inner wall of the sample tube (5) in a crescent shape; the baffle (51) divides the sample tube (5) into an upper and lower layer; a through hole is formed in the center of the baffle (51) to penetrate the upper and lower layers of the sample tube (5); and the narrow sides at both ends of the baffle (51) are not connected.

3. An auxiliary device for bovine tuberculosis test kit detection according to claim 2, characterized in that: A plurality of outer tube clamps (52) are correspondingly arranged on the outer wall of the sample tube (5), and a plurality of inner tube clamps (43) are correspondingly arranged on the inner wall of the culture hole (42); the outer tube clamps (52) and the inner tube clamps (43) are both L-shaped and mutually compatible.

4. The auxiliary device for bovine tuberculosis test kit detection according to claim 1, characterized in that: The vibration assembly (2) comprises a plurality of vibration tubes (21), the vibration tubes (21) being arranged in a tubular shape at the corners of the box body (1), each of the vibration tubes (21) being sleeved with a power rod (22) and a spring (23), and a groove being provided on one side of the vibration tube (21) close to the support assembly (3); One end of the power rod (22) is fixedly connected to the top of the inner wall of the box body (1), and the other end is in contact with the support assembly (3). The power rod (22) is used to control the downward movement of the support assembly (3); One end of the spring (23) is connected to the bottom of the box body (1), and the other end is connected to the support assembly (3). The spring (23) is used to control the support assembly (3) to reset upward.

5. An auxiliary device for bovine tuberculosis test kit detection according to claim 4, characterized in that: The support assembly (3) comprises an inner frame (31), a support frame (32), and a ball head (33); the support frame (32) is fixedly connected to the inner frame (31), the inner frame (31) is adapted to the storage box (4), a ball head (33) is provided at one end of the support frame (32) close to the vibration assembly (2), the ball head (33) is spherically clamped in the vibration tube (21), the support frame (32) passes through a groove of the vibration tube (21), and the ball head (33) moves up and down in the vibration tube (21).

6. An auxiliary device for bovine tuberculosis test kit detection according to claim 5, characterized in that: A stabilizing component (6) is provided between the storage box (4) and the inner frame (31), and the stabilizing component (6) is used to stabilize the storage box (4).

7. An auxiliary device for bovine tuberculosis test kit detection according to claim 1, characterized in that: A plurality of light-emitting diodes (44) are arranged in the storage box (4), and the plurality of light-emitting diodes (44) correspond to the culture holes (42) one by one, and the corresponding light-emitting diodes (44) are located on the bottom plate of the storage box (4) directly below the center of the culture hole (42); a photosensitive switch is arranged on the inner wall of each culture hole (42), and the corresponding photosensitive switch is electrically connected to the light-emitting diode (44).

8. The auxiliary device for bovine tuberculosis test kit detection according to claim 1, characterized in that: The box body (1) is provided with an opening at the top, and a transparent top cover is provided on the opening.

9. The auxiliary device for bovine tuberculosis test kit detection according to claim 5, characterized in that: The box body (1) is provided with a temperature display panel (11), and the inner wall of the inner frame body (31) is provided with a constant temperature component.

10. A method for using an auxiliary device for bovine tuberculosis test kit detection according to any one of claims 1 to 9, characterized in that: The steps include: S1, sample preparation: fix the storage box (4) in the inner frame (31), prepare a sterile and clean sample tube (5); aseptically collect bovine whole blood and put it into a blood collection tube containing anticoagulant blood, gently invert it upside down 5 to 10 times to fully mix the blood sample, and then add each portion of anticoagulant blood into the sample tube (5); S2, placing the sample tube (5) into the culture well (42), rotating the sample tube (5) counterclockwise, and the outer tube clamp (52) and the inner tube clamp (43) are engaged and clamped, so that the sample tube (5) and the well plate (41) are relatively locked, and then turning on the photosensitive switch and the light-emitting diode (44); S3, adding solution, tilting and rotating the sample tube (5) so that the wide part of the baffle (51) faces upward, and at this time, the inner wall of the sample tube (5) is smooth and passes through the baffle (51) from top to bottom; Observe the sample tube (5) from above the culture hole (42), and quickly determine whether a blood sample solution is currently added to the sample tube (5) based on the on / off status of the light-emitting diode (44), so as to prevent omission, wrong addition, repeated addition, etc.; S4, sample mixing, a plurality of power rods (22) are sequentially activated to perform telescopic movements, when the power rods (22) are extended, the ball heads (33) are driven to move downwards in sequence, when the power rods (22) are sequentially contracted, the springs (23) push the ball heads (33) to return upwards in sequence; the ball heads (33) drive the support frame (32) to move up and down in sequence, and then drive the inner frame (31) to shake in the box body (1), so that the blood sample in the sample tube (5) is fully mixed; S5, blood culture, after the blood sample is fully mixed, the constant temperature component is turned on, so that the temperature in the auxiliary device of the bovine tuberculosis test kit is set to 37° C. and cultured for 16-20 hours to obtain the blood culture supernatant; S6, taking out the sample, taking out the orifice plate (41) from the inner frame (31), and then rotating the sample tube (5) clockwise to separate the outer tube clamp (52) from the inner tube clamp (43), and lifting the sample tube (5) to remove the sample tube (5) from the orifice plate (41); S7, separation of the supernatant, tilting and rotating the sample tube (5) so that the wide part of the baffle plate (51) of the sample tube (5) faces downward, and extracting the blood sample using an extraction device such as a pipette, the supernatant is blocked by the baffle plate (51) in the upper layer of the baffle plate (51) and does not mix with the sample in the lower layer; the mixing of the blood sample and the supernatant can be effectively avoided, thereby improving the detection accuracy and efficiency.

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