Honeysuckle compound microorganism feed additive and application thereof

Abstract

The invention provides a honeysuckle compound microorganism feed additive and application thereof, and belongs to the technical field of feed. The invention provides a livestock and poultry feed additive, which comprises a honeysuckle extract and / or probiotics, and concretely verifies the growth performance improvement effect of the honeysuckle extract, bacillus licheniformis and the honeysuckle extract and bacillus licheniformis on livestock and poultry, and the result shows that compared with a control group, the growth performance improvement effect of the honeysuckle extract and the bacillus licheniformis on livestock and poultry is obviously improved. After the honeysuckle flower extract group, the probiotics and the honeysuckle flower extract and the probiotics are added, ADG and ADFI of the broiler chickens can be increased, F / G can be reduced, and therefore the effect of improving the growth performance of the broiler chickens is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and particularly relates to a honeysuckle composite microbial feed additive and application thereof. Background Art

[0002] In recent years, the side effects of antibiotics as growth promoters in the feed industry have seriously affected food safety and human health. With the continuous release of a series of policy conditions such as "banning antibiotics", "reducing and limiting antibiotics", and "carbon neutrality", it is particularly important to find alternatives to antibiotics. New microecological preparations such as probiotics, enzyme preparations, and plant extracts have become a research hotspot because of their outstanding advantages such as green, safe, and ecological, as well as biological functions such as improving production performance, improving intestinal health, and enhancing body immunity.

[0003] Honeysuckle (Lonicerajaponica Thunb., LJ), also known as honeysuckle, has a history of thousands of years and has high medicinal value. Honeysuckle is rich in flavonoids, organic acids, volatile oils, iridoid glycosides and other functionally active ingredients, among which the main active ingredients are chlorogenic acid, luteolin and the like. Honeysuckle has a broad-spectrum antibacterial effect on Staphylococcus aureus, Mycobacterium tuberculosis, Salmonella, Shigella dysenteriae, Proteus, Salmonella typhi, Salmonella paratyphi, Vibrio cholerae and other livestock and poultry pathogens. Honeysuckle also has antiviral ability. For example, the flavonoids and caffeoylquinic acid compounds it contains have very good anti-avian influenza virus activity, and luteolin has a good effect on viral diseases such as hepatitis, tonsillitis, and mumps. In addition, honeysuckle also has the effects of promoting animal growth, regulating intestinal health, improving the body's immunity, and enhancing the body's disease resistance and antioxidant capacity. Bacillus licheniformis is one of the commonly used probiotics in animal production. It has the functions of balancing intestinal flora, improving animal immunity and digestive enzyme activity, promoting animal growth, and can also replace antibiotics. In animal farming, Bacillus licheniformis can not only be used alone, but also used in combination with other probiotics, such as in poultry, ruminants and other animals with Bacillus subtilis, to improve animal growth performance, regulate intestinal flora, and improve the body's intestinal health.

[0004] However, there is no research on the combined use of honeysuckle and probiotics. Summary of the invention

[0005] The present invention provides a honeysuckle composite microbial feed additive and its application. The present invention verifies single product tests such as honeysuckle extract and probiotics, and finds that adding honeysuckle and / or probiotics to the diet has a promoting effect on the growth performance of broilers.

[0006] The first object of the present invention is to provide a feed additive comprising a honeysuckle extract and / or probiotics, wherein the types of the probiotics include Bacillus licheniformis, Lactobacillus plantarum and Clostridium butyricum.

[0007] Preferably, the composition comprises a honeysuckle extract and Bacillus licheniformis.

[0008] Preferably, based on the feed quality, the added amount of Bacillus licheniformis powder is 100 g / t, and the added amount of honeysuckle extract is 400-800 mg / kg.

[0009] The second object of the present invention is to provide a livestock and poultry feed, wherein the livestock and poultry feed contains the above feed additive.

[0010] The third object of the present invention is to provide the use of the above feed additive or the above livestock and poultry feed in improving the growth performance of livestock and poultry.

[0011] Preferably, the indicators for evaluating the growth performance of livestock and poultry include: daily weight gain, daily feed intake and feed-to-weight ratio.

[0012] The last object of the present invention is to provide a method for improving the growth performance of livestock and poultry, comprising feeding a feed containing the above-mentioned feed additive, or feeding the above-mentioned livestock and poultry feed.

[0013] Beneficial effects: In the embodiment of the present invention, broiler feeding is taken as an example, and the feeding management is carried out according to the "Kebao Broiler Feeding Management Manual". Drinking water and feeding are free, and immunization is carried out according to the conventional broiler immunization procedure. The results show that compared with the control group, the average daily weight gain of broilers in the groups with 400 and 600 mg / kg honeysuckle extract increased by 10.21% and 11.22% respectively, and the feed-to-weight ratio decreased by 6.52% and 7.07% respectively. This shows that the addition of honeysuckle to the diet has a promoting effect on the growth performance of broilers; compared with the control group, the addition of 1.0×10 8 ~10 9 CFU / kg of Bacillus licheniformis can increase ADG and ADFI and reduce F / G in broilers; adding Bacillus licheniformis and honeysuckle extract to the feed can improve the growth performance of broilers. DETAILED DESCRIPTION

[0014] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0015] In the present invention, the honeysuckle extract is provided by our unit, and the main component is chlorogenic acid.

[0016] The Bacillus licheniformis powder was first evenly mixed with the raw materials in a stepwise dilution manner to 10 kg, and then added to the feed according to the formula and stirred thoroughly.

[0017] Example 1

[0018] The experiment adopted a single-factor experimental design. 320 healthy, uniformly weighted one-day-old Cobb broiler chickens were selected and randomly divided into 4 groups. Each treatment had 8 replicates, each replicate had 10 chickens, and the experimental period was 42 days.

[0019] The treatment groups were as follows: ① control group, fed with basic diet; ② fed with basic diet + 200mg / kg honeysuckle extract; ③ fed with basic diet + 400mg / kg honeysuckle extract; ④ fed with basic diet + 600mg / kg honeysuckle extract. Feeding and management were carried out according to the "Cobb Broiler Feeding and Management Manual", with free access to water and food, and immunization was carried out according to the conventional broiler immunization procedure.

[0020] During the experiment, the experimental broilers were weighed on an empty stomach in repeated units, and the feed amount, residual feed amount, initial body weight and final body weight of the broilers at each stage were accurately recorded. The average daily gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F / G) of each group of broilers were calculated.

[0021] The results are shown in Table 1. Compared with the control group, the average daily weight gain of broilers in the groups supplemented with 400 and 600 mg / kg honeysuckle extract increased by 10.21% and 11.22% respectively, and the feed-to-weight ratio decreased by 6.52% and 7.07% respectively. This shows that the addition of honeysuckle to the diet has a promoting effect on the growth performance of broilers.

[0022] Table 1 Effect of honeysuckle extract on growth performance of broiler chickens

[0023]

[0024] Example 2

[0025] The experiment adopted a single-factor experimental design. A total of 240 healthy, uniformly-weighted one-day-old Cobb broiler chickens were selected and randomly divided into three groups. Each treatment had eight replicates, with 10 chickens in each replicate. The experimental period was 42 days.

[0026] The treatment groups were as follows: ① control group, fed with the basic diet; ② fed with the basic diet + 1.0×10 8 CFU / kg Bacillus licheniformis; ③ Feeding the basic diet + 1.0×10 9 CFU / kg Bacillus licheniformis. Feeding and management were carried out according to the "Cobb Broiler Feeding and Management Manual", with free access to water and food, and immunization was carried out according to the conventional broiler immunization procedure.

[0027] During the experiment, the experimental broilers were weighed on an empty stomach in repeated units, and the feed amount, residual feed amount, initial body weight and final body weight of the broilers at each stage were accurately recorded. The average daily gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F / G) of each group of broilers were calculated.

[0028] The results are shown in Table 2. Compared with the control group, the addition of 1.0×108~109 CFU / kg Bacillus licheniformis can increase broiler ADG and ADFI and reduce F / G.

[0029] Table 2 Effects of Bacillus licheniformis on growth performance of broiler chickens

[0030]

[0031]

[0032] Example 3

[0033] The same method as in Example 1 was used to feed the control group with a basic diet, and the experimental groups were fed with 10 8 ~10 9 CFU / kg Bacillus licheniformis, Lactobacillus plantarum and Clostridium butyricum. Feeding and management were carried out according to the "Cobb Broiler Feeding and Management Manual", with free access to water and food, and immunization was carried out according to the conventional broiler immunization program.

[0034] The results are shown in Table 3. The ADG of broilers in the Bacillus licheniformis group and the Clostridium butyricum group increased significantly, and the F / G value decreased significantly. Clostridium butyricum is a strict anaerobic bacterium with high nutritional requirements and fermentation conditions; while Bacillus licheniformis is an aerobic and facultative anaerobic bacterium with low nutritional requirements and fermentation conditions. Therefore, Bacillus licheniformis was selected as a probiotic for compatibility with honeysuckle extract.

[0035] Table 3 Effects of adding different probiotics to the diet on the growth performance of broilers

[0036]

[0037] Example 4

[0038] Using the same method as in Example 1, the control group (Group I) was fed with a basal diet, and the experimental groups were fed with experimental diets supplemented with 100 g / t Bacillus licheniformis + 400 mg / kg honeysuckle extract (Group II), 100 g / t Bacillus licheniformis + 600 mg / kg honeysuckle extract (Group III), and 100 g / t Bacillus licheniformis + 800 mg / kg honeysuckle extract (Group IV).

[0039] The results are shown in Table 4. Adding Bacillus licheniformis and honeysuckle extract to the diet can improve the growth performance of broiler chickens.

[0040] Table 4 Effects of Bacillus licheniformis and honeysuckle extract on the growth performance of broiler chickens

[0041]

[0042]

[0043] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A feed additive, characterized in that: The invention comprises honeysuckle extract and / or probiotics, wherein the types of the probiotics include Bacillus licheniformis, Lactobacillus plantarum and Clostridium butyricum.

2. The feed additive according to claim 1, characterized in that: Includes honeysuckle extract and Bacillus licheniformis.

3. The feed additive according to claim 2, characterized in that: Based on the feed quality, the addition amount of Bacillus licheniformis powder is 100g / t, and the addition amount of honeysuckle extract is 400-800mg / kg.

4. A livestock and poultry feed, characterized in that: The livestock and poultry feed contains the feed additive according to any one of claims 1 to 3.

5. Use of the feed additive according to any one of claims 1 to 3 or the livestock and poultry feed according to claim 4 in improving the growth performance of livestock and poultry.

6. The use according to claim 5, characterized in that: The indicators for evaluating the growth performance of the livestock and poultry include: daily weight gain, daily feed intake and feed-to-weight ratio.

7. A method for improving the growth performance of livestock and poultry, characterized in that: The method comprises feeding a feed containing the feed additive according to any one of claims 1 to 3, or feeding the livestock and poultry feed according to claim 4.

Images