Non-antibiotic antibacterial growth-promoting feed additive for animals and application of non-antibiotic antibacterial growth-promoting feed additive

Abstract

The invention discloses a non-antibiotic antibacterial growth-promoting feed additive for animals and application of the non-antibiotic antibacterial growth-promoting feed additive. The invention relates to a TBHQ (tert-butyl hydroquinone)-carvacrol emulsion which comprises the following components in percentage by weight: 25.0 to 35.0 percent of TBHQ, 25.0 to 35.0 percent of carvacrol, 3.0 to 10.0 percent of vanillin, 25.0 to 40.0 percent of vegetable oil, 0.05 to 0.1 percent of neotame and 2.0 to 5.0 percent of emulsifying solubilizer. The feed is suitable for species including but not limited to pigs, chickens, ducks, quails, pigeons, rabbits, sheep, cattle, fishes, shrimps and the like. In the aspect of improving the immunocompetence and production performance of livestock and poultry, the invention shows an excellent effect, and provides a solid technical basis for non-antibiotic bacteriostatic feed additives.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and in particular to a non-antibiotic antibacterial growth-promoting feed additive for animals and an application thereof. Background Art

[0002] In the scientific research field of the 21st century, the research on alternatives to antibiotics has become a key issue in global public health and sustainable development of animal husbandry. It is mainly used to treat infectious diseases caused by various bacteria and pathogenic microorganisms, and shows significant inhibitory or killing effects on a variety of microorganisms such as bacteria, fungi, rickettsia, mycoplasma, chlamydia, etc. Since the 1950s, antibiotics have gradually become popular in the field of animal husbandry as feed additives, aiming to prevent bacterial infections, improve feed conversion rates and promote animal growth, especially when animals are in a subclinical disease state, they can still maintain the stability of their production performance. Since the 1990s, the definition of antibiotics has expanded from traditional microbial secondary metabolites to biopharmaceuticals including synthetic analogs.

[0003] However, after more than half a century of extensive use, although feed antibiotic additives have made an indelible contribution to the development of animal husbandry, especially intensive animal husbandry, the accompanying biological and environmental problems such as bacterial resistance have become increasingly prominent. The implementation of this policy marks the transformation of animal husbandry to a natural and healthy breeding model, which is an inevitable trend in the development of the industry.

[0005] In view of the above background, in order to protect human health and promote the sustainable development of the aquaculture industry, finding efficient and safe alternatives to antibiotics has become an urgent task in the current scientific research field. Faced with the challenge of banning antibiotics, scientific researchers are committed to finding effective alternatives to ensure the healthy development of the aquaculture industry. At present, the research on alternatives is mainly concentrated in the fields of microecological preparations, acidifiers, potassium diformate, oligosaccharides, antimicrobial peptides and Chinese herbal medicines.

[0006] These alternatives show potential in antibacterial and bacteriostatic effects, improving immunity, and promoting growth, but they also face challenges such as lack of obvious effects and high costs, making it difficult to completely replace the effects of antibiotics.

[0007] In summary, the research progress of antibiotic substitutes shows that although a variety of potential substitutes have been proposed and applied in practice, in order to achieve comprehensive replacement of antibiotics, more in-depth exploration and innovation are still needed at the scientific research and application levels. This not only involves the development of new substitutes, but also includes the improvement of the effectiveness of existing substitutes and the reduction of costs, so as to achieve green, healthy and sustainable development of animal husbandry. Summary of the invention

[0008] 1. Technical issues to be resolved

[0009] The purpose of the present invention is to provide a non-antibiotic antibacterial growth-promoting feed additive for animals and application thereof.

[0010] (II) Technical solution

[0011] The invention provides a non-antibiotic antibacterial growth-promoting feed additive for animals, which comprises, by total amount percentage, 25.0-35.0% of TBHQ, 25.0-35.0% of carvacrol, 3.0-10.0% of vanillin, 25.0-40.0% of vegetable oil, 0.05-0.1% of neotame and 2.0-5.0% of emulsifying solubilizer.

[0012] Furthermore, by total percentage, TBHQ: 25.0%, carvacrol: 30.0%, vanillin: 5.0%, vegetable oil: 35.0%, neotame: 0.05%, emulsifying solubilizer: 4.95%.

[0013] Furthermore, TBHQ: 30.0%, carvacrol: 27.0%, vanillin: 8.0%, vegetable oil: 30.0%, neotame: 0.07%, emulsifying solubilizer: 4.93%.

[0014] Furthermore, TBHQ: 35.0%, carvacrol: 25.0%, vanillin: 10.0%, vegetable oil: 26.0%, neotame: 0.07%, emulsifying solubilizer: 3.93%.

[0015] Further, TBHQ: 29.98.0%, carvacrol: 29.98%, vanillin: 6.01%, vegetable oil: 29.98%, neotame: 0.08%, emulsifier: 3.97%

[0016] Furthermore, the emulsifying solubilizer is any one of polyoxyethylene 40 hydrogenated castor oil or polysorbate 80 or a mixture of the two.

[0017] The invention discloses an application of a non-antibiotic antibacterial growth-promoting feed additive for animals, which is added into the daily diet, and the daily dosage accounts for 0.05-0.15% of the weight of the daily diet.

[0018] Furthermore, the dosage for young animals can be appropriately increased to 0.08-0.15% of the daily diet weight, and the dosage for adult animals can be adjusted to 0.05-0.12% of the daily diet weight.

[0019] Furthermore, it is used as a dietary supplement in the process of raising pigs, chickens, ducks, quails, pigeons, rabbits, sheep, cattle, fish or shrimps.

[0020] (III) Beneficial effects

[0021] The present invention relates to a non-antibiotic antibacterial feed additive designed for a variety of animals, suitable for species including but not limited to pigs, chickens, ducks, quails, pigeons, rabbits, sheep, cattle, fish and shrimps. The additive has a wide range of applications and has passed strict safety and reliability tests.

[0022] The non-antibiotic antibacterial growth-promoting feed additive of the present invention has unique aromatic properties, and at the same time has significant antibacterial, antibacterial, mildew-proof, anti-stress and disease-preventing functions, and can effectively enhance the physical fitness of animals and promote their healthy growth and development.

[0023] The invention has shown excellent effects in improving the immunity and production performance of livestock and poultry, and provides a solid technical foundation for non-antibiotic antibacterial feed additives. Studies have shown that the product has significant advantages in improving animal immunity and improving production efficiency, indicating that it has broad application prospects in modern animal husbandry.

[0024] It should be understood that within the scope of the present invention, the above-mentioned various technical features of the present invention and the various technical features specifically described in the following embodiments can be combined with each other to form a new or preferred technical solution. Due to space limitations, they will not be described one by one here. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] An embodiment of the present invention provides a non-antibiotic antibacterial growth-promoting feed additive for animals, which includes, by total amount percentage: 25.0-35.0% TBHQ, 25.0-35.0% carvacrol, 3.0-10.0% vanillin, 25.0-40.0% vegetable oil, 0.05-0.1% neotame, and 2.0-5.0% emulsifier solubilizer.

[0027] Example 1: Calculated by total amount, TBHQ: 25.0%, carvacrol: 30.0%, vanillin: 5.0%, vegetable oil: 35.0%, neotame: 0.05%, emulsifying solubilizer: 4.95%.

[0028] Example 2: TBHQ: 30.0%, carvacrol: 27.0%, vanillin: 8.0%, vegetable oil: 30.0%, neotame: 0.07%, emulsifying solubilizer: 4.93%.

[0029] Example 3: TBHQ: 35.0%, carvacrol: 25.0%, vanillin: 10.0%, vegetable oil: 26.0%, neotame: 0.07%, emulsifying solubilizer: 3.93%.

[0030] Example 4: TBHQ: 29.98.0%, carvacrol: 29.98%, vanillin: 6.01%, vegetable oil: 29.98%, neotame: 0.08%, emulsifying solubilizer: 3.97%

[0031] Preferably, the emulsifying solubilizer is any one of RH-40 polyoxyethylene 40 hydrogenated castor oil or T-80 polysorbate 80 or a mixture of the two.

[0032] The invention discloses a non-antibiotic antibacterial growth-promoting feed additive for animals, which is added to the diet, and the daily dosage accounts for 0.05-0.15% of the diet weight. The dosage for young animals can be appropriately increased to 0.08-0.15% of the diet weight, and the dosage for adult animals can be adjusted to 0.05-0.12% of the diet weight. In the present invention: Adult Animals

[0033] definition:

[0034] Adult animals are animals that have passed their rapid growth period and reached sexual maturity. At this point, the animal's growth rate slows down and its immune system is relatively stable, but it still needs to maintain good health and production performance. Depending on the animal species, the specific time range of the adult stage is as follows:

[0035] Pigs: over 6 months old (approximately 25 kg in weight).

[0036] Chicken: broilers over 6 weeks old, laying hens over 18 weeks old.

[0037] Duck: 7 weeks old and above.

[0038] Quail: 4 weeks old and above.

[0039] Pigeons: 6 weeks old and above.

[0040] Rabbit: 12 weeks and above.

[0041] Sheep: 6 months and above.

[0042] Cattle: 12 months and above (adult cattle).

[0043] Fish: over 10 cm in length.

[0044] Shrimp: Body length exceeds 3-5 cm.

[0045] Juvenile Animals

[0046] definition:

[0047] Young animals usually refer to the period from birth to sexual maturity. During this period, animals are in a period of rapid growth and development, have higher nutritional needs, and their immune systems are not fully developed, making them more susceptible to diseases. Depending on the animal species, the specific time range of the young stage is as follows:

[0048] Pigs: From weaning to about 6 months of age (about 20-25 kg body weight).

[0049] Chickens: 1 day to 6 weeks of age (broilers), or 1 day to 18 weeks of age (laying hens).

[0050] Duck: 1 day to 7 weeks old.

[0051] Quail: 1 day to 4 weeks old.

[0052] Pigeons: 1 day old to 6 weeks old.

[0053] Rabbits: Weaning to 12 weeks of age.

[0054] Sheep: Weaning to 6 months of age.

[0055] Cattle: weaning to 12 months of age (calves).

[0056] Fish: After hatching, the body length reaches about 10 cm.

[0057] Shrimp: From the larval stage until the body length reaches about 3-5 cm.

[0058] The invention discloses an application of a non-antibiotic antibacterial growth-promoting feed additive for animals, which is used for adding into the daily diet during the feeding process of pigs, chickens, ducks, quails, pigeons, rabbits, sheep, cattle, fish or shrimps.

[0059] First, the present invention tests TBHQ and carvacrol, which have good bactericidal and antibacterial effects on both Gram-negative and Gram-positive bacteria:

[0060] The following table shows the minimum inhibitory concentration (MIC)

[0061]

[0062] The following table shows the minimum bactericidal concentration (MBC). At the test concentration, the bactericidal rate is 99.99% / 5 minutes.

[0063]

[0064] First, from the perspective of the minimum inhibitory concentrations for different bacteria, carvacrol and TBHQ showed certain antibacterial ability against a variety of bacteria. For Escherichia coli K99, K88 and Salmonella typhimurium among Gram-negative bacteria, the minimum inhibitory concentrations of carvacrol and TBHQ were relatively close, about 1.6mM and 3.2mM, respectively. In Salmonella pullorum, the minimum inhibitory concentration of TBHQ was relatively high, at 6.4mM. For Pseudomonas aeruginosa, the minimum inhibitory concentrations of carvacrol and TBHQ were both greater than 6.4mM, indicating that the antibacterial effects of these two ingredients on Pseudomonas aeruginosa were relatively weak. Among Gram-positive bacteria, Staphylococcus aureus also had different sensitivities to carvacrol and TBHQ, with the minimum inhibitory concentration of carvacrol being 1.6mM and that of TBHQ being 80μM. For Lactobacillus rhamnosus and Lactobacillus plantarum, carvacrol and TBHQ also had certain effects, and the concentrations were relatively low.

[0065] Secondly, from the perspective of the minimum bactericidal concentration, taking Escherichia coli K99 as an example, carvacrol is 1.6mM and TBHQ is 3.2mM, which shows that when a certain concentration is reached, these two ingredients can effectively kill bacteria.

[0066] TBHQ is a substance widely used in food and edible oil antioxidant preservation. In this feed additive, it can not only play a bactericidal and antibacterial role, but also has the function of feed preservation. Carvacrol can be used alone or in combination due to its good bactericidal and antibacterial effects. Different bacteria have different sensitivities to these two ingredients, which provides ideas for rationally adjusting the formula of feed additives in practical applications according to different animal breeding environments and possible bacterial species. For example, for animals that are susceptible to Escherichia coli infection, the ratio of carvacrol and TBHQ can be appropriately increased; and for situations that may be less threatened by Pseudomonas aeruginosa, the dependence on these two ingredients in inhibiting Pseudomonas aeruginosa can be relatively reduced. In addition, by comparing with traditional antibiotics such as gentamicin, it can also be seen that the ingredients in this feed additive have certain potential and unique advantages in antibacterial and bactericidal effects, providing new directions and possibilities for finding alternatives to antibiotics.

[0067] Example 5: The preparation process of a non-antibiotic antibacterial growth-promoting feed additive for animals of the present invention is as follows:

[0068] Add 34.0 kg of vegetable oil, 28.0 kg of TBHQ, 30.0 kg of carvacrol, 5.0 kg of vanillin, 0.1 kg of neotame and 2.9 kg of T-80 into a high-speed mixer in sequence, mix at 50±5°C for 20 minutes, then filter, remove foam, measure and package.

[0069] Sensitivity test of the non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5 to Escherichia coli and Salmonella:

[0070] In order to evaluate the antibacterial effect of a non-antibiotic antibacterial growth-promoting feed additive for animals prepared in Example 5 of the present invention, we conducted a sensitivity test and compared it with similar commercial products on the market, Quinocetone and Zinc Bacitracin. All tests were conducted in strict accordance with the 2015 edition of the General Principles 1201 standard of the Chinese Pharmacopoeia.

[0071] Test subjects:

[0072] Feed additive prepared in Example 5 of the present invention

[0073] Similar products on the market

[0074] Quinocetone (antibiotic control)

[0075] Bacitracin zinc (antibiotic control)

[0076] Test strains:

[0077] Escherichia coli

[0078] Salmonella

[0079] Test method:

[0080] The inhibition zone method was used to determine the inhibitory effect of each sample on the above two pathogens. The antibacterial ability was evaluated according to the diameter of the inhibition zone. The larger the inhibition zone, the stronger the antibacterial effect.

[0081] Results:

[0082] The following table shows the comparison results of the size of the inhibition zone of Escherichia coli and Salmonella in different treatment groups (unit: mm). The difference in the antibacterial efficacy between the treatment groups can be intuitively seen through the comparison.

[0083]

[0084] Analysis and discussion:

[0085] The feed additive prepared in Example 5 and the same type of commercial products: For Escherichia coli, the diameter of the antibacterial zone of Example 5 is 22 mm, while the diameter of the commercial product is 16 mm. This shows that the product of Example 5 has a stronger antibacterial ability. For Salmonella, the diameter of the antibacterial zone of Example 5 is 21 mm, while the diameter of the commercial product is 15 mm, which also shows a stronger antibacterial effect.

[0086] Feed additives and antibiotics (quinocetone and bacitracin zinc) prepared in Example 5: For Escherichia coli, the diameter of the inhibition zone of Example 5 is slightly smaller than that of quinocetone (23 mm), but significantly larger than that of bacitracin zinc (13 mm). This shows that the product of Example 5 is close to the traditional antibiotic quinocetone in terms of antibacterial effect, and far exceeds that of bacitracin zinc. For Salmonella, the diameter of the inhibition zone of Example 5 is 21 mm, slightly smaller than 22 mm of quinocetone, but still significantly better than 11 mm of bacitracin zinc.

[0087] Comparison between antibiotics: Quinocetone showed strong antibacterial ability against both bacteria, with the diameter of the inhibition zone being 23mm (Escherichia coli) and 22mm (Salmonella), the highest among all tested samples. The antibacterial effect of bacitracin zinc was relatively weak, especially against Salmonella, with the diameter of the inhibition zone being only 11mm.

[0088] Conclusion: The non-antibiotic antibacterial and growth-promoting feed additive prepared in Example 5 demonstrated strong antibacterial ability, especially when compared with similar products on the market. Although quinocetone showed the best antibacterial effect as a traditional antibiotic, the product of Example 5 almost reached the same level in antibacterial performance, showing its great potential as an antibiotic substitute. In contrast, bacitracin zinc had the weakest antibacterial effect, especially against Salmonella. These results not only verify the effectiveness of the feed additive prepared in Example 5, but also emphasize its potential application value as a non-antibiotic antibacterial agent in animal husbandry, which helps to reduce dependence on antibiotics and promote the development of green breeding.

[0089] Determination and comparison of minimum inhibitory concentration (MIC)

[0090] In order to evaluate the antibacterial efficacy of a non-antibiotic antibacterial growth-promoting feed additive for animals prepared in Example 5 of the present invention, and to compare it with similar commercial products on the market and traditional antibiotic quinocetone, we conducted a minimum inhibitory concentration (MIC) test. All tests were conducted in strict accordance with the 2015 edition of the General Principles 1201 standard of the Chinese Pharmacopoeia.

[0091] Test subjects:

[0092] Non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5

[0093] Similar products on the market

[0094] Quinocetone

[0095] Test strains:

[0096] Escherichia coli

[0097] Test method:

[0098] The minimum inhibitory concentration (MIC) of each sample against Escherichia coli was determined by microdilution method. The lowest drug concentration that can completely inhibit bacterial growth was determined by gradually reducing the sample concentration.

[0099] Results:

[0100] The table below shows the minimum inhibitory concentration (in g / ml) of different treatment groups against Escherichia coli.

[0101]

[0102] Analysis and discussion:

[0103] The non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5 exhibited a significantly lower MIC value (20 μg / ml), which was much lower than the 110 μg / ml of the same type of commercial products and the 53 μg / ml of quinocetone. This indicates that the product in Example 5 has a stronger antibacterial effect and can effectively inhibit the growth of Escherichia coli at a lower concentration.

[0104] In addition, compared with quinocetone, the additive prepared in Example 5 not only exhibited a lower MIC value, but also demonstrated its advantage as a non-antibiotic antibacterial agent. This result further verifies the superiority of the additive in antibacterial performance and its great potential as an antibiotic substitute.

[0105] Conclusion: In summary, the non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5 showed excellent antibacterial efficacy against Escherichia coli, and its MIC value was significantly better than that of similar products on the market and traditional antibiotic quinocetone. These findings not only prove the effectiveness of the additive, but also highlight its potential application value in reducing antibiotic dependence and promoting green farming.

[0106] In order to verify the effects of the antibiotic-free feed antibacterial growth-promoting additive for animals of the present invention and the traditional antibiotic feed additive in feeding performance and disease resistance, the following experiment was conducted.

[0107] Experiment 1:

[0108] Experimental materials and methods

[0109] Experimental animals: 80 healthy three-way piglets (Duroc×Landrace×Large White) weighing about 10 kg were selected from a pig farm. The pigs were randomly divided into an experimental group and a control group, with 40 pigs in each group. Each group was further divided into two replicate groups, with 20 pigs in each replicate group. The experimental period was from October 8, 2015 to November 23, 2015, and the location was Xiamen.

[0110] Feed source: Experimental group: fed with ordinary diet and the non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5 (the added amount was 0.08% of the weight of the ordinary diet).

[0111] Control group: fed with ordinary diet + antibiotics (bacitracin zinc + colistin, added at 100 g / ton and 20 g / ton respectively). Bacitracin zinc was produced by Shenzhou Veterinary Drug Co., Ltd., Hebei Province, and colistin was produced by Shandong Lukang Pharmaceutical Factory.

[0112] Feeding and management: The experimental pig house has good ventilation and insulation conditions, and the house temperature is maintained at 18-22℃. The pre-trial period is 3 days, and the formal trial period is 45 days. During the trial, the pigs can eat and drink freely, and are managed according to the conventional feeding and management procedures of the pig farm.

[0113] Production performance measurement: The pigs were weighed on an empty stomach at the beginning of the experiment and before the group transfer on the 35th day, the feed consumption of each group was recorded, and the average daily gain (ADG) and average daily feed intake (ADFI) were calculated.

[0114] The number of pigs in each group that were sick within 35 days before the experiment and the incidence within 10 days after the group transfer were recorded.

[0115] Test results and analysis:

[0116] Growth performance: In terms of growth performance, the average head weight gain and average daily weight gain of the experimental group of pigs were 9.8% higher than those of the control group, with significant differences (p<0.05). In addition, the feed-to-meat ratio of the experimental group was reduced by 5.5%, which also showed a significant difference (p<0.05).

[0117] Disease resistance: In terms of disease resistance, compared with the control group, the number of sick pigs in the test group decreased by 140% within 35 days before the test, and the number of sick pigs in the 10 days after the transfer decreased by 152%, with extremely significant differences (p<0.01). This shows that the test group performed well in disease resistance and stress resistance.

[0118] Conclusion: In summary, this study shows that the non-antibiotic antibacterial growth-promoting feed additive for animals of the present invention not only significantly improves the growth performance of pigs, but also enhances their disease resistance. This result verifies the scientific rationality of the additive formula and its significant feeding effect in actual breeding, showing great potential as an alternative to antibiotics.

[0119] Experiment 2:

[0120] Experimental animals: 1,200 one-day-old Ross broiler chicks provided by a chicken farm were randomly divided into two groups, the experimental group and the control group, with 600 chicks in each group, and then further divided into three replicate groups, with 200 chicks in each replicate group. The experimental time was from October 10, 2016 to November 20, 2016, and the location was Quanzhou.

[0121] Feed source: Experimental group: fed with compound feed and the non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5 (the added amount was 0.06% of the weight of the ordinary diet).

[0122] Control group: fed with compound feed + antibiotics (salinomycin, 50 grams per ton of compound feed). Compound feed is divided into two stages: 1-21 days old is the early stage of broilers, and 22 days old to market is the late stage of broilers.

[0123] Feeding and management: The chicken house has good ventilation and insulation conditions, and the house temperature is controlled as follows:

[0124] The temperature was maintained at 28-30℃ from 1 to 18 days of age, and gradually decreased from 19 days of age to 20-22℃ after 21 days of age. The trial period was 40 days. During the trial, broilers had free access to food and water and were managed according to the conventional feeding and management procedures of the farm.

[0125] Production performance measurement: Weigh the pigs on the 21st and 40th days, record the feed consumption and mortality of each experimental group and each replicate group, and calculate the average daily gain (ADG), average daily feed intake (ADFI), feed to meat ratio (F / G) and survival rate.

[0126] Test results and analysis:

[0127] Growth performance: At 21 days of age: the average body weight and survival rate of the experimental group increased by 5.3% and 1.8% respectively compared with the control group, with significant differences (p<0.05). The feed-to-meat ratio decreased by 6.5%, with significant differences (p<0.05).

[0128] At 40 days of age: the average body weight of the experimental group was 3.2% higher than that of the control group, with a significant difference (p<0.05). The feed-to-meat ratio was reduced by 6.9%, with an extremely significant difference (p<0.01). The survival rate was increased by 2.9%, with an extremely significant difference (p<0.01).

[0129] Disease resistance: Throughout the trial period, the test group showed lower incidence and higher survival rate, indicating that it has stronger disease resistance and stress resistance.

[0130] Conclusion: In summary, this study shows that feed containing the non-antibiotic antibacterial growth-promoting feed additive of the present invention not only significantly improves the growth performance of broilers, but also enhances their disease resistance. This result verifies the scientific rationality of the additive formula and its significant feeding effect in actual breeding, showing great potential as an alternative to antibiotics.

[0131] Experiment 3:

[0132] Experimental animals: 800 Ross chickens of the same age were selected from a chicken farm and randomly divided into two groups, the experimental group and the control group, with 400 chickens in each group. The experimental period was from July 10 to October 30, 2006, and the location was Zhangzhou.

[0133] Feed source: Experimental group: fed with compound feed and the non-antibiotic antibacterial growth-promoting feed additive prepared in Example 5 (the added amount was 0.06% of the weight of the ordinary diet).

[0134] Control group: fed only with compound feed.

[0135] Feeding and management: Two groups of chickens are kept in the same house, using triple-stacked ladder cages, automatic watering fountains for water supply, automatic feces cleaning once a day in the morning and evening; free access to complete feed, disinfected once a week. Feed is added twice a day at 07:00-07:30 in the morning and 17:30-18:00 in the afternoon, natural ventilation, and 16 hours of light per day. Full-time breeders regularly pick up eggs and observe the appetite, feces and health of the chickens, and keep records.

[0136] Test results and analysis: The test results show that the average egg production rate of the test group is 6.2% higher than that of the control group. The difference is extremely significant (p<0.01) after multiple comparisons by q method. This shows that the non-antibiotic antibacterial growth-promoting feed additive of the present invention has a significant effect in improving the egg production rate of laying hens.

[0137] Summary: The above studies show that the non-antibiotic antibacterial growth-promoting feed additive of the present invention exhibits excellent performance in both broiler and laying hen feeding, not only improving growth rate and egg production rate, but also enhancing disease resistance, proving its great potential as an alternative to antibiotics. These findings provide new solutions for green farming and help reduce dependence on antibiotics.

[0138] In the present invention:

[0139] Tertiary Butylhydroquinone (TBHQ) Chemical name: 2-tert-butyl-4,6-dimethylphenol.

[0140] Function: TBHQ is a highly effective antioxidant that can extend the shelf life of products, maintain their freshness, and reduce odor and color changes caused by oxidation. In the present invention, TBHQ helps protect the nutrients in feed from being destroyed by oxidation.

[0141] Chemical name of carvacrol: 3-methyl-5-(1-propenyl)-2-cyclohexen-1-ol.

[0142] Function: Carvacrol is an essential oil component extracted from natural plants such as mint and parsley, which has strong antibacterial and antifungal activity. It can effectively inhibit the growth and reproduction of various pathogenic microorganisms and give the product a pleasant aroma. In the present invention, carvacrol is used as the main antibacterial component to enhance the antibacterial properties of the feed and improve its odor.

[0143] Chemical name of vanillin: 4-hydroxy-3-methoxybenzaldehyde.

[0144] Function: Vanillin is a common edible spice with a strong vanilla aroma. It can not only improve the palatability of feed, making animals more willing to eat, but also mask the unpleasant taste or odor that other ingredients may bring. In addition, vanillin also has a certain antioxidant effect, which helps to stabilize the quality of feed.

[0145] Sources of vegetable oil: It can come from a variety of plant seeds or fruits, such as soybean oil, corn oil, sunflower oil, etc.

[0146] Function: Vegetable oil is one of the important sources of energy, providing essential fatty acids to support the growth and development of animals. It can also help dissolve fat-soluble vitamins (such as A, D, E, and K) and promote the absorption of these nutrients. In the present invention, vegetable oil acts as a carrier, allowing other active ingredients to be evenly dispersed in the feed, while increasing the energy density of the feed.

[0147] Chemical name of Neotame: N-[N-(3,3-dimethylbutyl)-L-α-aspartyl]-L-phenylalanine 1-methyl ester.

[0148] Function: Neotame is a high-intensity artificial sweetener with a sweetness of about 7000-13000 times that of sucrose. Since it can achieve a significant sweetening effect at a very low dosage, neotame will not affect the energy content of the feed, but can significantly improve the flavor of the feed and increase the feed intake of animals. In the present invention, neotame is used as a flavoring agent to enhance the palatability of the feed without adding extra calories.

[0149] The emulsifying solubilizer is RH-40 or T-80.

[0150] Function: Emulsifiers and solubilizers are mainly used to improve the compatibility and stability between different ingredients, ensuring that the oil phase and the water phase can be evenly mixed to form a stable emulsion. They can prevent the occurrence of stratification and ensure the uniform distribution of additives in the entire feed system. In addition, some emulsifiers and solubilizers also have slight antibacterial or antioxidant properties, further enhancing the overall quality of the feed. Raw materials such as TBHQ, carvacrol, vanillin, vegetable oil, neotame and emulsifiers and solubilizers are all raw materials approved for use by the state, which are safe and reliable. The addition of neotame can improve palatability, increase animal feed intake and thus promote growth.

[0151] Synergistic relationship between ingredients in the formula

[0152] The non-antibiotic antibacterial growth-promoting feed additive of the present invention is composed of tertiary butylhydroquinone (TBHQ), carvacrol, vanillin, vegetable oil, neotame and emulsifying solubilizer. These ingredients not only play a unique role each, but also further enhance the overall effect of the product through synergistic effect.

[0153] Antioxidant and antibacterial synergistic effects

[0154] TBHQ and carvacrol: TBHQ, as a highly effective antioxidant, can protect the nutrients in feed from being destroyed by oxidation and extend the shelf life of the product. Carvacrol has significant antibacterial and antifungal activity and can inhibit the growth and reproduction of pathogenic microorganisms. The combination of the two can not only prevent the quality degradation caused by oil oxidation, but also effectively control the growth of harmful microorganisms, thereby improving the safety and stability of the feed.

[0155] Synergistic effect for improved palatability

[0156] Vanillin and Neotame: Vanillin gives the feed a strong vanilla flavor and improves its smell; Neotame, as a high-intensity sweetener, can significantly increase the sweetness of the feed at a very low dosage without adding extra calories. The two work together to greatly enhance the palatability of the feed, making animals more willing to eat, thereby increasing feed intake and production performance.

[0157] Synergy of nutrition and function

[0158] Vegetable oil and carvacrol: Vegetable oil is not only an important source of energy, providing essential fatty acids to support animal growth and development, but also helps dissolve fat-soluble vitamins and promotes nutrient absorption. The dispersion of carvacrol in vegetable oil allows its antimicrobial properties to be more evenly distributed throughout the feed system, ensuring that each particle has an antimicrobial effect without affecting the physical properties or taste of the feed.

[0159] The synergy of stability and uniformity

[0160] Emulsifiers and vegetable oils: Emulsifiers such as lecithin and polysorbate improve the compatibility and stability between different ingredients, ensuring that the oil phase and the water phase are evenly mixed to form a stable emulsion. This not only prevents the occurrence of stratification, ensures the uniform distribution of additives in the entire feed system, but also promotes the effective release and utilization of other active ingredients.

[0161] Synergy of comprehensive benefits

[0162] All ingredients: A complex network of interactions is formed between the above ingredients, which not only play their best performance, but also achieve the following comprehensive benefits through synergistic effects: Enhanced antibacterial effect, the synergistic effect of multiple antibacterial ingredients (such as carvacrol) improves the overall antibacterial ability. Improve feed stability, the combination of antioxidants (such as TBHQ) and emulsifiers and solubilizers ensures the stability of feed quality over a long period of time. Improve palatability and feed intake: The addition of spices (such as vanillin) and sweeteners (such as neotame) enhances the attractiveness of feed and increases the animal's desire to eat. Optimize nutrient absorption, the combination of vegetable oils and other ingredients promotes the efficient absorption of nutrients and supports the healthy growth of animals.

[0163] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article or device.

[0164] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that not every embodiment includes only one independent technical solution, and in the absence of a conflict of solutions, the various technical features mentioned in each embodiment can be combined in any manner to form other implementation methods that can be understood by those skilled in the art.

[0165] In addition, without departing from the scope of the present invention, the technical solutions described in the aforementioned embodiments may be modified, or some of the technical features may be replaced by equivalents, without causing the essence of the corresponding technical solutions to depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A non-antibiotic antibacterial growth-promoting feed additive for animals, calculated by percentage of total amount, comprising: TBHQ 25.0-35.0%, carvacrol 25.0-35.0%, vanillin 3.0-10.0%, vegetable oil 25.0-40.0%, neotame 0.05-0.1%, emulsifying solubilizer 2.0-5.0%.

2. The non-antibiotic antibacterial growth-promoting feed additive for animals according to claim 1, characterized in that: Calculated by total amount, TBHQ: 25.0%, carvacrol: 30.0%, vanillin: 5.0%, vegetable oil: 35.0%, neotame: 0.05%, emulsifying solubilizer: 4.95%.

3. The non-antibiotic antibacterial growth-promoting feed additive for animals according to claim 1, characterized in that: TBHQ: 30.0%, carvacrol: 27.0%, vanillin: 8.0%, vegetable oil: 30.0%, neotame: 0.07%, emulsifying solubilizer: 4.93%.

4. The non-antibiotic antibacterial growth-promoting feed additive for animals according to claim 1, characterized in that: TBHQ: 35.0%, carvacrol: 25.0%, vanillin: 10.0%, vegetable oil: 26.0%, neotame: 0.07%, emulsifying solubilizer: 3.93%.

5. The non-antibiotic antibacterial growth-promoting feed additive for animals according to claim 1, characterized in that: TBHQ: 29.98.0%, carvacrol: 29.98%, vanillin: 6.01%, vegetable oil: 29.98%, neotame: 0.08%, emulsifying solubilizer: 3.97%.

6. A non-antibiotic antibacterial growth-promoting feed additive for animals according to any one of claims 1 to 4, characterized in that: The emulsifying solubilizer is any one of polyoxyethylene 40 hydrogenated castor oil and polysorbate 80 or a mixture of the two.

7. The use of a non-antibiotic antibacterial growth-promoting feed additive for animals as claimed in any one of claims 1 to 4, characterized in that: Added in the diet, the daily dosage accounts for 0.05-0.15% of the diet weight.

8. The use of a non-antibiotic antibacterial growth-promoting feed additive for animals as claimed in claim 7, characterized in that: The dosage for young animals can be appropriately increased to 0.08-0.15% of the daily diet weight, and the dosage for adult animals can be adjusted to 0.05-0.12% of the daily diet weight.

9. The use of a non-antibiotic antibacterial growth-promoting feed additive for animals as claimed in any one of claims 1 to 4, characterized in that: Used as a dietary supplement during the feeding process of pigs, chickens, ducks, quails, pigeons, rabbits, sheep, cattle, fish or shrimps.

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