A composition for enhancing immunity of pets and a preparation method and application thereof

Abstract

The application discloses a composition for enhancing the immunity of pets and a preparation method thereof, and comprises the following raw materials in parts by weight: 25-40 parts of Echinacea purpurea powder, 5-8 parts of petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer, 35-55 parts of willow bark extract, and 60-80 parts of a carrier. The composition of the application effectively improves the immunity of pets, increases the weight of pets, and promotes the growth of pets by compounding the Echinacea purpurea powder, the petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer and the willow bark extract, and synergistic effect. Meanwhile, the beef extract as the carrier also has the effects of supplementing nutrition and enhancing constitution, and can enhance the effect of the composition of the application to a certain extent.

Description

Technical Field

[0001] This invention belongs to the field of pet food, specifically relating to a composition for enhancing the immune system of pets, its preparation method, and its application. Background Technology

[0002] As society develops, people not only pursue material things but also value spiritual enjoyment. Pets are animals and plants kept for human spiritual purposes; they are humanity's most loyal friends and emotional anchors for many. Therefore, more and more families are keeping pets, and pets have become important members of many households, forging deep friendships with their owners and adding much joy to family life.

[0003] Most pets today are fed regular food, occasionally with treats. Regular food and treats have simple ingredients, and their nutritional components cannot fully meet the nutritional needs of pets' growth and development. Long-term use can disrupt the intestinal environment, affect the immune system, reduce immunity, and lead to problems such as loss of appetite, bloating, and even diarrhea. Furthermore, when pets are sick, antibiotics are commonly used, which carries significant risks. Large amounts of antibiotics, once ingested, circulate throughout the body via the bloodstream, gradually weakening the body's immunity and reducing antigenic immunogenicity, directly affecting the immune response mechanism. In addition, antibiotic use can lead to antibiotic resistance in pathogens, and some stable antibiotics can enter the environment through feces, causing significant environmental pollution. Therefore, providing a rationally formulated composition that can effectively improve pet immunity has become an urgent problem to be solved. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition for enhancing the immune system of pets, its preparation method and application.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A first aspect of the present invention provides a composition for enhancing the immune system of pets, comprising the following raw materials in parts by weight:

[0007] The ingredients are: 25-40 parts echinacea powder, 5-8 parts petunia-3-trans-p-coumaryl rutin-5-glucoside monomer, 35-55 parts willow bark extract, and 60-80 parts carrier; the carrier is beef extract.

[0008] Preferably, the method for preparing the echinacea powder includes the following steps:

[0009] Fresh whole Echinacea plants are air-dried at 23-26℃ for 15-20 hours, then pulverized and passed through a 100-mesh sieve to obtain Echinacea powder.

[0010] Preferably, the willow bark extract is prepared by the following method:

[0011] (1) The willow bark was placed in an ethanol solution and extracted by ultrasonication. The residue and the first filtrate were obtained by filtration.

[0012] (2) Put the filter residue from step (1) into water for a second extraction and filter to obtain the second filtrate;

[0013] (3) The first filtrate and the second filtrate were mixed and concentrated under reduced pressure to obtain willow bark extract.

[0014] Preferably, the volume concentration of ethanol in step (1) is 70%, the mass ratio of willow bark to ethanol solution is 1kg:4-15L, and the conditions for ultrasonic extraction are: ultrasonic power 200-1000W, extraction temperature 40-60℃, and extraction time 20-25min.

[0015] Preferably, the water in step (2) is pure water, deionized water or distilled water, and the water solution is added according to the ratio of filter residue mass to water solution volume of 1kg:4~15L; the conditions for the second extraction are: extraction temperature 90~100℃, extraction time 1~5h.

[0016] A second aspect of the present invention provides a method for preparing the above-mentioned composition for enhancing the immune system of pets, comprising the following steps:

[0017] The above composition is mixed evenly to make a nutritional paste.

[0018] A third aspect of the present invention provides the use of the above-described composition for enhancing the immune system of pets in improving the immune system of pets.

[0019] The preferred application method is as follows:

[0020] Add the above composition to pet food or feed at a rate of 5-10% of the weight of the pet food or feed.

[0021] The beneficial effects of this invention are:

[0022] This invention provides a composition for enhancing the immune system of pets. By combining echinacea powder, petunidin-3-trans-p-coumaryl rutin-5-glucoside monomer, and willow bark extract, the synergistic effect effectively improves the pet's immunity, increases weight, and promotes growth. Simultaneously, the beef extract, acting as a carrier, also provides nutritional supplementation and strengthens the body, further enhancing the overall effect of the composition. Detailed Implementation

[0023] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0024] As mentioned earlier, the raw materials for pet food or treats are currently simple, and long-term use can cause intestinal environmental disorders, affect the body's immune system, and reduce its own immunity. At the same time, the antibiotic treatment usually used when pets are sick can further weaken the pet's immunity, cause pathogens to develop drug resistance, and enter the environment with feces, causing environmental pollution.

[0025] To address the aforementioned problems, this invention provides a composition capable of enhancing pet immunity. In the selection of raw materials, the inventors used antifungal and antiviral traditional Chinese medicinal herbs as criteria, screening for Coptis chinensis, Phellodendron chinense, Sophora flavescens, Lonicera japonica, Echinacea purpurea, and Taraxacum mongolicum; and Andrographis paniculata, Astragalus membranaceus, Salix babylonica bark, and Leonurus japonicus as criteria for analgesia and anti-inflammation; and garlic, Ginkgo biloba leaves, and anthocyanins as criteria for antioxidant activity. Then, based on the criteria of suitability for addition to pet food, the following were obtained: Phellodendron chinense, Sophora flavescens, Lonicera japonica, Echinacea purpurea, and Taraxacum mongolicum; Astragalus membranaceus, Leonurus japonicus, and Salix babylonica bark; garlic and anthocyanins.

[0026] After combining and comparing the data from the three groups of raw materials, it was found that the synergistic effect among willow bark extract, echinacea, and anthocyanins was the best, effectively improving the pet's immunity and weight. Finally, based on the criteria of supplementing nutrition and enhancing physical fitness, this invention selected beef extract as the carrier.

[0027] In Examples 2-4 and Comparative Examples 1-3 below, the preparation method of the petunidin-3-trans-p-coumaryl rutin-5-glucoside monomer used refers to the invention patent CN107118244B. The echinacea powder and willow bark extract used were prepared in Example 1. The beef extract was purchased from Sichuan Baoshun Biotechnology Co., Ltd.

[0028] Example 1: Preparation of raw materials

[0029] (1) Preparation of Echinacea powder:

[0030] Fresh whole Echinacea plants were air-dried at 25°C for 15 hours, then pulverized and passed through a 100-mesh sieve to obtain Echinacea powder.

[0031] (2) Preparation of willow bark extract:

[0032] Under the conditions of a willow bark mass-to-ethanol solution volume ratio of 1 kg:4 L, 40℃, and ultrasonic power of 200 W, willow bark was ultrasonically extracted in a 70% ethanol solution. The residue and filtrate were obtained by filtration. The filtrate was concentrated under reduced pressure to obtain an ethanol extract of willow bark. The residue was then subjected to a second extraction with deionized water under the following conditions: temperature 90℃, extraction time 1 h, and a residue-to-water solution volume ratio of 1 kg:4 L. The second extraction was then filtered and the residue was discarded to obtain an aqueous extract of willow bark. Finally, the ethanol extract and aqueous extract of willow bark were mixed and concentrated under reduced pressure to obtain a willow bark extract.

[0033] Example 2: Preparation of a composition for enhancing the immune system of pets

[0034] According to the weight proportions, 25 parts of echinacea powder, 5 parts of petunidin-3-trans-p-coumaroyl rutin-5-glucoside monomer, 35 parts of willow bark extract and 60 parts of beef extract are evenly mixed to prepare a nutritional paste.

[0035] Example 3: Preparation of a composition for enhancing the immune system of pets

[0036] According to the weight proportions, 30 parts of echinacea powder, 6 parts of petunia-3-trans-p-coumaroyl rutin-5-glucoside monomer, 40 parts of willow bark extract and 70 parts of beef extract are evenly mixed to prepare a nutritional paste.

[0037] Example 4: Preparation of a composition for enhancing the immune system of pets

[0038] According to the weight proportions, 40 parts of echinacea powder, 8 parts of petunia-3-trans-p-coumaroyl rutin-5-glucoside monomer, 55 parts of willow bark extract and 80 parts of beef extract are evenly mixed to prepare a nutritional paste.

[0039] Comparative Example 1:

[0040] According to the weight proportions, 35 parts of willow bark extract and 60 parts of beef extract were evenly mixed to prepare a nutritional paste.

[0041] Comparative Example 2:

[0042] According to the weight proportions, 25 parts of echinacea powder and 60 parts of beef extract are evenly mixed to prepare a nutritional paste.

[0043] Comparative Example 3:

[0044] According to the weight proportions, 5 parts of petunia-3-trans-p-coumaryl rutin-5-glucoside monomer and 60 parts of beef extract were evenly mixed to prepare a nutritional paste.

[0045] Experimental example:

[0046] (1) Experimental method:

[0047] Twenty-five healthy, disease-free cats (Linqing Lion Cats) of similar weight and age were selected and quarantined for one week after purchase, and then vaccinated with the feline triple vaccine. They were then randomly divided into five treatment groups of five cats each. Each group was fed 10g of feline cat food at 8:00, 12:00, and 18:00 daily. The specific grouping method is as follows:

[0048] Control group: fed regular cat food.

[0049] Experimental Group 1: The nutritional paste prepared in Example 2 was added to the regular cat food, at a rate of 5% of the weight of the regular cat food.

[0050] Experimental Group 2: The nutritional paste prepared in Comparative Example 1 was added to the regular cat food, and the amount added was 5% of the weight of the regular cat food.

[0051] Experimental Group 3: The nutritional paste prepared in Comparative Example 2 was added to the regular cat food, at a rate of 5% of the weight of the regular cat food.

[0052] Experimental Group 4: The nutritional paste prepared in Comparative Example 3 was added to the regular cat food, at a rate of 5% of the weight of the regular cat food.

[0053] Regular cat food mainly consists of the following ingredients by weight: 50kg dehydrated chicken, 10kg corn kernels, 30kg chicken fat, 40kg wheat, 20kg dehydrated duck liver, 2kg minerals, 10kg fish oil, 15kg yeast, 0.8kg trace elements, 16kg rice, 8kg taurine, 13kg corn flour, 5kg plant fiber, 19kg vegetable oil, 6kg egg powder, 0.2kg L-lysine, and 0.03kg ethoxyquin (preservative).

[0054] After 10 weeks of feeding, the cats were weighed, and FCV / FHV / FPV antibodies were detected using an FCV / FHV / FPV rapid detection kit (purchased from Weifang Nobixin Biotechnology Co., Ltd.). The changes and average values ​​were calculated.

[0055] The method of using the test reagent is as follows:

[0056] 1. Turn on the instrument. Check that the three ID chips match the batch number of the reagent kit.

[0057] 2. Insert a product ID chip into the instrument's ID card slot. After automatic reading, confirm that the reagent kit name and batch number are correct.

[0058] 3. Take 10 μL of LEDTA anticoagulated plasma and add it to the diluent, then invert the container to mix thoroughly.

[0059] 4. After mixing, immediately pipette 100 μL of the above mixed liquid and add it to the sample well of the test card.

[0060] 5. After adding the sample, let it react for 10 minutes, then insert the test card into the instrument slot and click "Quick Test" to get the test results; or after adding the sample, immediately insert the test card into the instrument slot and click "Standard Test" to wait for the test results.

[0061] 6. Read and print the test results from the instrument's display screen.

[0062] (2) Experimental results:

[0063] Comparison of data from the control group, experimental group 1, and experimental groups 2-4 shows that the present invention, through the synergistic effect of the combination of echinacea powder, petunidin-3-trans-coumaroyl rutin-5-glucoside monomer, and willow bark extract, effectively increases the weight of pets and enhances their immunity.

[0064] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. The use of the pet composition in the preparation of products that increase the levels of FCV IgG, FHV IgG and FPV IgG in cats, characterized in that, The pet composition comprises the following raw materials in parts by weight: 25-40 parts echinacea powder, 5-8 parts petunidin-3-trans-p-coumaryl rutin-5-glucoside monomer, 35-55 parts willow bark extract, and 60-80 parts carrier; wherein the carrier is beef extract. The echinacea powder is prepared by the following method: fresh whole echinacea plants are air-dried at 23-26℃ for 15-20 hours, and then pulverized and passed through a 100-mesh sieve to obtain echinacea powder; The willow bark extract was prepared by the following method: (1) The willow bark was placed in an ethanol solution and extracted by ultrasonication. The residue and the first filtrate were obtained by filtration. (2) Put the filter residue from step (1) into water for a second extraction and filter to obtain the second filtrate; (3) The first and second filtrates were mixed and concentrated under reduced pressure to obtain willow bark extract; In step (1), the volume concentration of ethanol is 70%, the mass ratio of willow bark to ethanol solution is 1kg:4-15L, and the conditions for ultrasonic extraction are: ultrasonic power 200-1000W, ultrasonic time 1-100s, interval 1-100s, extraction temperature 40-60℃, and extraction time 20-25min. The water in step (2) is deionized water or distilled water, and the water solution is added at a ratio of 1 kg: 4 to 15 L of filter residue mass to water solution volume. The conditions for the second extraction are: extraction temperature 90 to 100℃ and extraction time 1 to 5 h.

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