Anti-shedding and oil control dandruff-removing composition and preparation method thereof

Abstract

The application discloses a composition for preventing hair loss, controlling oil and removing dandruff and a preparation method thereof, and belongs to the field of cosmetic formulations. The composition for preventing hair loss, controlling oil and removing dandruff comprises the following components: 2-5 parts of an Artemisia argyi extract, 1.1-1.6 parts of a ginger root extract, 5-10 parts of a Polygonum multiflorum extract and 4-6 parts of a Platycladus orientalis extract. The Polygonum multiflorum extract prepared by the method has good bacteriostatic activity, and mixed strain fermentation can more effectively enrich active ingredients in the Polygonum multiflorum than single strain fermentation. The composition for preventing hair loss, controlling oil and removing dandruff has excellent oil control and oil inhibition effects. The shampoo provided by the application has the effect of significantly preventing hair loss and fixing hair.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of toiletry preparations, and particularly relates to a composition for preventing hair loss, controlling oil and removing dandruff and a preparation method thereof. BACKGROUND

[0002] Hair loss, excessive sebum secretion and dandruff problems are common concerns of consumers regarding scalp health. These problems not only affect personal appearance, but also can have a negative impact on an individual's psychology and social interactions.

[0003] Firstly, hair loss has multiple causes, including physiological shedding and pathological shedding. Physiological shedding is a normal metabolic process of hair, and the number of hair shed daily is within a certain range. However, when hair is excessively shed, beyond the normal range, it forms pathological hair loss. The causes of pathological hair loss are complex and diverse, including genetics, mental stress, physical and chemical stimuli, infection, endocrine disorders, malnutrition and seasonal changes, etc. These causes lead to damage to hair follicles, which in turn affects the normal growth and development of hair.

[0004] Secondly, excessive sebum secretion on the scalp is also a common problem. The scalp is rich in sebaceous glands, and excessive secretion of sebum can stimulate the scalp keratinization to accelerate, forming excessive dandruff. At the same time, excessive sebum can also block the hair follicles, affecting the normal respiration and nutrient absorption of hair, further exacerbating the problem of hair loss.

[0005] Thirdly, dandruff is also a common concern of consumers. The production of dandruff is related to the imbalance of the scalp microbiota, especially the overgrowth of Malassezia. Malassezia is a lipophilic fungus that uses sebum secreted by the scalp as a source of nutrition, and overgrowth can stimulate the scalp to produce inflammatory reactions, leading to the production of dandruff.

[0006] In order to solve these problems, various anti-hair loss, oil control and dandruff removal products have appeared on the market. However, these products often have the problems of insignificant effect, strong irritation, easy dependence, etc. Therefore, it is particularly important to develop a product with significant anti-hair loss, oil control and dandruff removal effect, and safety, mildness and non-irritation. SUMMARY

[0007] In view of the deficiencies of the prior art, the present application aims to provide an efficacy component with significant anti-hair loss, oil control and dandruff removal effect, and safety, mildness and non-irritation, and a shampoo thereof.

[0008] In order to achieve the above-mentioned purpose, the present application discloses the following technical solutions:

[0009] In the first aspect, the present application provides a composition for preventing hair loss, controlling oil and removing dandruff, comprising the following components by mass fraction:

[0010] mulberry leaf extract 2-5 parts;

[0011] ginger root extract 1.1-1.6 parts;

[0012] foxtail millet extract 5-10 parts;

[0013] Chinese arborvitae leaf extract 4-6 parts;

[0014] The preparation method of the foxtail millet extract comprises the following steps:

[0015] S1-1. The foxtail millet is crushed and sieved to obtain foxtail millet powder, which is mixed with deionized water at a solid-liquid ratio of 1:5 g / mL, heated to 80-90°C and kept for 10 h, and then cooled to room temperature to obtain a solid-liquid mixture;

[0016] S1-2. The Bacillus subtilis is inoculated into a seed culture medium, cultured in a shaker at a speed of 100-125 r / min at 28-30°C for 24-36 h, and then a Bacillus subtilis bacterial solution is obtained;

[0017] S1-3. The Bacillus velezensis is inoculated into a seed culture medium, cultured in a shaker at a speed of 100-125 r / min at 28-30°C for 24-36 h, and then a Bacillus velezensis bacterial solution is obtained;

[0018] S1-4. The Bacillus subtilis bacterial solution and the Bacillus velezensis bacterial solution are mixed at a volume ratio of (1.8-2):1 to obtain a mixed bacterial solution, which is mixed with the solid-liquid mixture at a ratio of 1:10 mL / g, and then fermented at a speed of 200-300 r / min, a temperature of 30±2°C and a ventilation volume of 1.5-2vvm for 24 h. After the fermentation is completed, the residue is removed by centrifugation and filtration, and the supernatant is obtained. The supernatant is subjected to low-temperature instantaneous sterilization treatment, and then the supernatant is subjected to spray drying to obtain the foxtail millet extract.

[0019] Preferably, the seed culture medium is prepared by mixing 5.0 g / L of proteose peptone, 3.0 g / L of beef extract, 5.0 g / L of NaCl, 0.005 g / L of MnSO4·H2O and 1 L of sterile water, sterilizing at 121°C for 15 min, and cooling.

[0020] Preferably, the viable bacterial count of the Bacillus subtilis bacterial solution is ≥10 6 CFU / mL.

[0021] Preferably, the viable bacterial count of the Bacillus velezensis bacterial solution is ≥10 6 CFU / mL.

[0022] Preferably, in the step S1-1, the foxtail millet is crushed and then sieved through a 60-100 mesh sieve.

[0023] Preferably, in the step S1-4, after the fermentation is completed, the supernatant is obtained by filtering off the residue under the condition of centrifugation at 8000 r / min for 25 min.

[0024] In a second aspect, the present application provides the use of the anti-hair loss, oil control and dandruff removal composition of the first aspect in the preparation of a hair care product with the effects of anti-hair loss, oil control and dandruff removal.

[0025] In a third aspect, the present application provides an anti-hair loss, oil control and dandruff removal shampoo, wherein the shampoo comprises the anti-hair loss, oil control and dandruff removal composition of the first aspect.

[0026] The anti-hair loss, oil control and dandruff removal composition is added in an amount of 0.5-2 wt% in the shampoo.

[0027] The shampoo further comprises a surfactant, a conditioning agent, a preservative, a humectant, a fragrance, an anti-dandruff agent, a thickening agent, a pH adjusting agent, a colorant, a cooling agent and a solvent.

[0028] Preferably, the surfactant is at least one of sodium laureth sulfate, cocamidopropyl betaine, sodium lauryl sulfate, cocamide MEA, quaternium-73, polysorbate-20, polyquaternium-52, laureth-16, TEA-dodecylbenzenesulfonate, guar hydroxypropyltrimonium chloride and C12-15 Pareth-3.

[0029] The conditioning agent is at least one of trihydroxystearin, hydrolyzed chitin, hydrolyzed corn starch, dimethiconol and dimethicone.

[0030] The preservative is at least one of p-hydroxyacetophenone, phenoxyethanol and sodium benzoate.

[0031] The humectant is at least one of 1,2-hexanediol, butylene glycol, glycerin, caprylyl glycol, propylene glycol and coconut oil.

[0032] The fragrance is a daily fragrance.

[0033] The anti-dandruff agent is piroctone olamine salt.

[0034] The thickening agent is at least one of sodium chloride and acrylates / acrylamide copolymer.

[0035] The pH adjusting agent is at least one of citric acid and ethanolamine.

[0036] The colorant is at least one of CI 15985, CI 16255 and CI 42090.

[0037] The cooling agent is menthol.

[0038] The solvent is deionized water.

[0039] In a fourth aspect, the application provides a preparation method of the anti-hair loss and oil control dandruff-removing shampoo of the third aspect, characterized in that it comprises the following steps:

[0040] The anti-hair loss and oil control dandruff-removing composition, the surfactant, the conditioning agent, the preservative, the humectant, the fragrance, the dandruff-removing agent, the thickening agent, the colorant, the cooling agent and the solvent are stirred and mixed uniformly to obtain the anti-hair loss and oil control dandruff-removing shampoo.

[0041] In the application:

[0042] The Artemisia leaf extract contains rich active ingredients such as volatile oil, flavonoids, tannins, polysaccharides, triterpenes, trace elements and chlorogenic acid, etc. The volatile oil has the effects of relieving asthma and inhibiting bacteria. The flavonoids, chlorogenic acid and sesquiterpenes have the effect of anti-inflammation. The tannins have the effects of astringing and hemostasis. Meanwhile, the Artemisia leaf extract has strong inhibitory effect on Malassezia, Escherichia coli and Staphylococcus aureus.

[0043] The ginger root extract contains rich gingerol, zingiberene, gingerol and ginger ketone, and amino acids, etc. It has the effects of inhibiting bacteria, relieving inflammation, anti-oxidation, promoting blood circulation of the scalp, promoting active growth of hair follicle cells, and assisting hair growth. It also has certain regulating effect on the secretion of scalp oil.

[0044] The extract of Radix Polygoni Multiflori contains active ingredients such as anthraquinones, stilbene glycosides and phospholipids, etc. It has the effects of promoting blood circulation of the scalp, promoting active growth of hair follicle cells, and promoting hair growth. It also has certain anti-inflammatory and antibacterial effects.

[0045] The extract of Platycladus Orientalis contains rich volatile oil, flavonoids and tannins, etc. It has the effects of inhibiting bacteria, anti-tumor, anti-inflammation, anti-erythrocyte oxidation, hemostasis, etc. It has unique effect in preventing hair loss. The volatile oil in the extract of Platycladus Orientalis has obvious inhibitory effect on Malassezia, Staphylococcus aureus, Micrococcus tetragenus, Escherichia coli and Aerobacter aerogens. The extract of Platycladus Orientalis can remove dandruff, promote hair regeneration, enhance the metabolic function of hair follicles, and has certain regulating effect on the secretion of scalp oil.

[0046] Microbial fermentation technology can effectively enrich the active ingredients in polygonum multiflorum, and enhance its pharmacological activity. The present application research found that by using bacillus subtilis and bacillus velezensis compound strains to ferment polygonum multiflorum, the physiological activities such as antioxidant, anti-inflammatory, antibacterial and oil control of polygonum multiflorum extract can be significantly increased. During the fermentation process, the content of anthraquinones and stilbene glucoside will increase, and new anthraquinone components will be produced by microbial metabolism.

[0047] The beneficial effects of the present application are:

[0048] 1. The polygonum multiflorum extract prepared by the method of the present application has good antibacterial activity, and the mixed strain fermentation can more effectively enrich the active ingredients in polygonum multiflorum than the single strain fermentation.

[0049] 2. The anti-hair loss, oil control and dandruff removal composition provided by the present application has excellent oil control and oil inhibition effects.

[0050] 3. The shampoo provided by the present application has the effect of significantly preventing hair loss and fixing hair. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical scheme and advantages of the present application more clear and explicit, the present application is further described in detail below in combination with specific embodiments. However, the following embodiments are only preferred embodiments of the present application, not all. In order to be clear, not all features of the actual embodiments are described.

[0052] Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0053] In the present application:

[0054] Artemisia argyi leaf extract: Artemisia argyi leaf extract, purchased from Xi'an Shennong Biological Technology Co., Ltd., specification 20:1;

[0055] Ginger root extract: Zingiber officinale root extract, purchased from Xi'an Shennong Biological Technology Co., Ltd., specification 20:1;

[0056] Polygonum multiflorum extract: polygonum multiflorum extract, dried polygonum multiflorum purchased from Guangzhou Qingping Medicine Wholesale Market;

[0057] Biota orientalis leaf extract: Biota orientalis leaf extract, purchased from Xi'an Shennong Biological Technology Co., Ltd., specification 20:1;

[0058] Bacillus subtilis: purchased from Guangdong Microbial Culture Collection Center, preservation number GDMCC NO.1.131;

[0059] Bacillus velezensis: purchased from Guangdong Microbial Culture Collection Center, preservation number GDMCC NO.1.135;

[0060] Other raw materials can be obtained from the market.

[0061] Preparation of the extract of Radix Polygoni Multiflori:

[0062] S1-1. Dry Radix Polygoni Multiflori was pulverized in a high-speed pulverizer, and passed through a 60-mesh sieve to obtain Radix Polygoni Multiflori powder. The Radix Polygoni Multiflori powder was mixed with deionized water at a solid-liquid ratio of 1:5 g / mL, heated to 90°C, and then kept at 90°C for 10 h to obtain a solid-liquid mixture.

[0063] S1-2. 0.5 cm 2 Bacillus subtilis slant was inoculated into the prepared seed culture medium, and cultured in a shaker at a speed of 125 r / min at 30°C for 30 h to obtain a Bacillus subtilis bacterial solution, with a viable bacterial count of 5×10 6 CFU / mL.

[0064] S1-3. 0.5 cm 2 Bacillus velezensis slant was inoculated into the prepared seed culture medium, and cultured in a shaker at a speed of 125 r / min at 28°C for 36 h to obtain a Bacillus velezensis bacterial solution, with a viable bacterial count of 5×10 6 CFU / mL.

[0065] S1-4. The Bacillus subtilis bacterial solution and the Bacillus velezensis bacterial solution were mixed at a ratio of 2:1 v / v to obtain a mixed bacterial solution. The mixed bacterial solution was mixed with the solid-liquid mixture obtained in step S1-1 at a ratio of 1:10 mL / g, and then placed in a fermentation tank. Fermentation was carried out at a speed of 200 r / min, a temperature of 30±2°C, and a ventilation rate of 2 vvm for 24 h. After fermentation, centrifugation was carried out at a speed of 8000 r / min for 25 min. The residue was removed by filtration, and the supernatant was subjected to low-temperature instantaneous sterilization treatment. Finally, the supernatant was subjected to spray drying to obtain the extract of Radix Polygoni Multiflori ①.

[0066] The seed culture medium was prepared by mixing 5.0 g / L of proteose peptone, 3.0 g / L of beef extract, 5.0 g / L of NaCl, 0.005 g / L of MnSO4·H2O, and 1 L of sterile water, and then sterilizing at 121°C for 15 min.

[0067] To demonstrate the superior properties of the above-prepared polygonum multiflorum extract, the following polygonum multiflorum extracts obtained by different preparation methods and commonly used commercially available polygonum multiflorum extracts are provided and numbered for distinction.

[0068] Polygonum multiflorum extract ②: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix Bacillus subtilis and Bacillus velezensis bacterial solutions at a ratio of 1.8:1 v / v, and the remaining steps and parameters are consistent.

[0069] Polygonum multiflorum extract ③: Dry polygonum multiflorum is ground in a high-speed grinder, sieved through a 60-mesh sieve to obtain polygonum multiflorum powder. The polygonum multiflorum powder is mixed with deionized water at a ratio of 1:5 g / mL, heated to 90°C, and then incubated for 10 hours. Then, the temperature is lowered to room temperature to obtain a solid-liquid mixture. Centrifuge at 8000 r / min for 25 min, filter the residue, and take the supernatant for low-temperature instant sterilization treatment, then spray drying to obtain polygonum multiflorum extract ③.

[0070] Polygonum multiflorum extract ④: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix only Bacillus subtilis bacterial solution with the solid-liquid mixture at a ratio of 1:10 mL / g for fermentation treatment, and the remaining steps and parameters are consistent.

[0071] Polygonum multiflorum extract ⑤: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix only Bacillus velezensis bacterial solution with the solid-liquid mixture at a ratio of 1:10 mL / g for fermentation treatment, and the remaining steps and parameters are consistent.

[0072] Polygonum multiflorum extract ⑥: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix Bacillus subtilis and Bacillus velezensis bacterial solutions at a ratio of 1:1 v / v, and the remaining steps and parameters are consistent.

[0073] Polygonum multiflorum extract ⑦: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix Bacillus subtilis and Bacillus velezensis bacterial solutions at a ratio of 3:1 v / v, and the remaining steps and parameters are consistent.

[0074] Polygonum multiflorum extract ⑧: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix Bacillus subtilis and Bacillus natto bacterial solutions at a ratio of 2:1 v / v, and the remaining steps and parameters are consistent.

[0075] Polygonum multiflorum extract ⑨: Refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix Bacillus subtilis and Bacillus licheniformis bacterial solutions at a ratio of 2:1 v / v, and the remaining steps and parameters are consistent.

[0076] Radix Polygoni Multifidi extract D: Radix Polygoni Multifidi extract C-II purchased from Guangzhou Luojie Biotechnology Co., Ltd.

[0077] The Bacillus natto bacterial liquid and the Bacillus licheniformis bacterial liquid are both prepared by conventional culture, and the viable bacterial count is 5×10 6 CFU / mL.

[0078] Preparation of the anti-shedding and oil-control dandruff composition:

[0079] The raw material components were weighed according to the formulation amount in Table 1, mixed in a mixing blender, and uniformly mixed to obtain the anti-shedding and oil-control dandruff composition.

[0080] Table 1 Raw materials of the anti-shedding and oil-control dandruff composition and their mass fractions

[0081]

[0082] Note: "-" in the table indicates no addition.

[0083] The above compositions 1-11 were mixed with deionized water to prepare sample solutions with a concentration of 2 wt%, and performance tests were performed.

[0084] Performance test of the anti-shedding and oil-control dandruff composition:

[0085] 1 Malassezia bacteria inhibition test

[0086] 1.1 Detection principle

[0087] Malassezia is one of the important factors for the formation of dandruff, and the growth and decline of Malassezia is directly related to the amount of dandruff. Malassezia furfur is a well-studied fungus in the genus Malassezia, which is isolated from human dandruff (head dandruff), and has a representative indicator. In this test, the sample containing the bacteriostatic agent is placed on the plate dyed with Malassezia furfur, and the sample is continuously dissolved to form different concentration gradients through agar diffusion to show its bacteriostatic effect. By measuring the size of the bacteriostatic ring, it can be determined whether it has bacteriostatic ability, and it is suitable for the identification of products containing bacteriostatic agents and dissolution-resistant (bacteriostatic) products.

[0088] 1.2 Judgment standard

[0089] The negative control sample should not produce a bacteriostatic ring. If the diameter of the bacteriostatic ring of the test sample is > 7 mm, it is judged to have bacteriostatic effect; if the diameter of the bacteriostatic ring is ≤ 7 mm, it is judged to have no bacteriostatic effect.

[0090] 1.3 Test method

[0091] Take sterile dry filter paper, and add 20 μL of sample solution to each piece to obtain test samples. The negative control sample is replaced with sterile water. Use a sterile cotton swab to dip the sample solution with a concentration of 5.0×105 CFU / mL ~ 5.0 x 10 6 CFU / mL Malassezia furfur suspension, evenly spread 3 times on the surface of the appropriate medium plate. Place 1 infected plate for each test, and place 4 test samples, 1 negative control sample, a total of 5 pieces on each plate. Cover the plate and place it in a (36 ± 1) °C incubator for 72 h. Observe the results and measure the diameter of the inhibition ring (including the sample) with a vernier caliper and record it.

[0092] 1.4 Test results

[0093] Table 2 Inhibition performance results of compositions 1-11

[0094]

[0095] 1.5 Analysis of results

[0096] According to the test results, the negative control sample has no inhibition ring, and the diameter of the inhibition ring of the tested sample is > 7 mm, indicating that all samples have the ability to inhibit Malassezia furfur. The inhibition performance of composition 2 is better than that of compositions 4-11, proving that the polygonum multiflorum extract prepared by the method of the present application has good inhibition activity. The specific strain compound fermentation provided by the present application can more effectively enrich the active ingredients in polygonum multiflorum than other strains and single strain fermentation.

[0097] 2 Oil control efficacy test

[0098] 2.1 Preparation of reagents

[0099] Test sample: As described above, prepare test sample solutions with a concentration of 2 wt% from the above-prepared compositions 1-11.

[0100] Testosterone (T) solution: Dissolve 36 mg of testosterone in anhydrous ethanol to prepare a testosterone solution with a concentration of 5 mmol / L;

[0101] NADPH (reduced coenzyme II) solution: Dissolve 42.5 mg of NADPH tetrasodium salt in water to prepare a NADPH solution with a concentration of 2 mmol / L;

[0102] Reaction solution: Dissolve DTT (dithiothreitol) and sodium phosphate in water to obtain a reaction solution with a final concentration of 1 mmol / L for DTT and 20 mmol / L for sodium phosphate.

[0103] 2.25 Determination of α-reductase activity

[0104] The reaction components are mixed in the order (reaction solution 155 muL, 37 DEG C constant temperature; testosterone solution 10 muL; NADPH solution 10 muL; 5 alpha-reductase 20 muL), continuously monitor the change of absorbance of reaction system at 340 nm within 4 min, the changing speed reflects the activity of 5 alpha-reductase (in the reaction system with temperature of 37 DEG C, the enzyme amount that makes the concentration of NADPH decrease 1 muMol / L per minute is an enzyme activity unit), and the initial enzyme activity is recorded.

[0105] 2.35 alpha-reductase inhibition rate determination

[0106] The reaction components are mixed in the order (reaction solution 155 muL, 37 DEG C constant temperature; testosterone solution 10 muL; NADPH solution 10 muL; 5 alpha-reductase 20 muL), continuously monitor the change of absorbance of reaction system at 340 nm within 4 min, the changing speed reflects the activity of 5 alpha-reductase (in the reaction system with temperature of 37 DEG C, the enzyme amount that makes the concentration of NADPH decrease 1 muMol / L per minute is an enzyme activity unit), and the initial enzyme activity is recorded.

[0107] Table 3 oil control effect result

[0108]

[0109]

[0110] 2.4 result analysis

[0111] 5 alpha-reductase is a membrane protein enzyme dependent on reduced coenzyme II (NADPH), is an important androgen metabolism enzyme in skin, can irreversibly convert testosterone into dihydrotestosterone, and DHT is the most active androgen, can induce sebaceous glands to excessively secrete oil.

[0112] According to the experimental results, the compositions 1-3 provided by the application have excellent oil control effect, wherein the oil control effect of the composition 2 is better than that of the compositions 1 and 3; according to the results of the compositions 4-11, it is proved that the polygonum multiflorum extract prepared by the method of the application has good oil control effect, and the bacillus subtilis liquid and bacillus velezensis liquid in the specific mixed strains provided by the application have excellent oil control performance in the specific preparation range, and compared with single strain and other strain compounding, the oil control effect is more excellent.

[0113] Preparation of shampoo:

[0114] S2-1. Weighing: accurately weigh each raw material component according to the formula amount in table 1;

[0115] S2-2. Feeding and heating: the A phase raw materials were added to the emulsifying kettle, mixed and stirred and heated to 85°C, the homogenizer was turned on, emulsified for 10 min, then the emulsifying homogenizer was turned off, stirring was continued for 20 min until complete dissolution, defoaming was performed for 30 min, and then cooling was started;

[0116] S2-3. Cooling to 60°C, the B phase raw materials were added to the emulsifying kettle, mixed and stirred uniformly, and then cooled to 50°C, the C phase raw materials were added to the emulsifying kettle in sequence and stirred for 20 min until uniform, and then the D phase raw materials were added to the emulsifying kettle in sequence at 45°C and stirred uniformly, and then the product was taken for inspection, and the product was discharged if qualified;

[0117] S2-4. Packaging: after discharging, the product was allowed to stand for 24 h, and then microbiological test was performed, and the product was filled by a filling machine after passing the test, and then the product was packaged into a finished product;

[0118] S2-5. Finished product inspection: after the finished product was packaged, physical and chemical tests and microbiological tests were performed, and then the product was sealed and stored in a warehouse after passing the tests.

[0119] Table 4 Raw materials of the shampoo and their mass percentages

[0120]

[0121]

[0122] Note: "-" in the table indicates no addition.

[0123] Human trial test:

[0124] I. Materials and methods

[0125] 1. Test article: Example 1 and Comparative Example 1.

[0126] 2. Subjects: A total of 30 people, 13 men and 17 women, aged 23 to 58 years, with an average age of 40.5 ± 11.5 years, who met the voluntary selection criteria for subjects.

[0127] 3. Test method: The subjects were directly used the test product according to the use characteristics and methods indicated on the label of the cosmetic product, and the subjects were observed or followed up by telephone once a week to observe the skin reactions, and the results were recorded according to the current effective technical specification for human trial test skin adverse reactions grading standard, and the test time was not less than 4 weeks.

[0128] II. Test results

[0129] Table 5 Results of the human trial test

[0130]

[0131] Result analysis: The results of the human trial test showed that there was no skin adverse reaction in 30 people.

[0132] Anti-drop performance test:

[0133] I. Materials and methods

[0134] 1. Test subject: Example 1.

[0135] 2. Test product: Example 1.

[0136] 3. Subjects: A total of 30 people, test group: 1 male, 29 females, aged 21-58 years, with an average age of 44.3±10.9 years, meeting the voluntary selection criteria of the subjects.

[0137] 4. Instruments and equipment

[0138] 4.1 Hair growth management system, model: DermoGenius ultra

[0139] 4.2 Digital camera, model: EOS850D

[0140] 5. Test method:

[0141] Volunteer subjects were recruited according to the selection criteria, and the number of hair loss was counted by 60 times of combing. Qualified subjects were given a 2-week washout period, and after the washout period, the 60 times of combing method was used again to count the number of hair loss. Those with more than 10 hair loss entered the formal test. The selected subjects were evaluated for the hair base value before using the product, including hair loss count, hair density evaluation and image shooting, and were recorded; the same evaluation and test were conducted again 4 weeks, 8 weeks and 12 weeks after using the product.

[0142] 6. Statistical method:

[0143] Statistical analysis software was used for statistical analysis of the data. The measurement data was expressed as: mean ± standard deviation, and normal distribution test was conducted. If it met the requirements of normal distribution, paired t test was used for comparison before and after itself, otherwise two related sample rank sum test was used. For comparison of rank data before and after, two related sample rank sum test was used. The above statistical analysis was two-tailed test, and the significance level was a=0.05.

[0144] II. Results and data analysis

[0145] Table 6 Analysis of time points before and after using the test product

[0146]

[0147] III. Test conclusion

[0148] Compared with before use, the hair loss count of the test product group did not significantly increase at 4 weeks after use, 8 weeks after use and 12 weeks after use, and the hair loss count was significantly lower than that at 0 week at 8 weeks after use and 12 weeks after use (P<0.05).

[0149] Compared with before use, the overall hair density of the test product group did not significantly decrease at 4 weeks after use, 8 weeks after use and 12 weeks after use, and the overall hair density was significantly higher than that at 0 week at 12 weeks after use (P<0.05).

[0150] Compared with before use, the local hair density of the test product group did not significantly decrease at 4 weeks after use, 8 weeks after use and 12 weeks after use, and the local hair density was significantly higher than that at 0 week at 4 weeks after use, 8 weeks after use and 12 weeks after use (P<0.05). The test product has the effect of preventing hair loss.

[0151] Although the specific embodiments of the present application are described above, those skilled in the art should understand that this is only an example, and the protection scope of the present application is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present application, and these changes and modifications all fall within the protection scope of the present application.

Claims

1. An anti-dandruff, control oil, and antidandruff composition, characterized in that, The composition consists of the following components in mass fraction: Artemisia leaf extract 2-5 parts; Ginger root extract 1.1-1.6 parts; Radix Polygoni Multiflori extract 5-10 parts; Biota leaf extract 4-6 parts; The preparation method of the Radix Polygoni Multiflori extract comprises the following steps: S1-1. The Radix Polygoni Multiflori is crushed and sieved to obtain Radix Polygoni Multiflori powder, which is mixed with deionized water at a solid-liquid ratio of 1:5 g / mL, heated to 80-90°C and kept for 10 h, and then cooled to room temperature to obtain a solid-liquid mixture; S1-2. Bacillus subtilis is inoculated in a seed culture medium, cultured in a shaker at a rotation speed of 100-125 r / min at 28-30°C for 24-36 h, and then a Bacillus subtilis bacterial solution is obtained; S1-3. Bacillus velezensis is inoculated in a seed culture medium, cultured in a shaker at a rotation speed of 100-125 r / min at 28-30°C for 24-36 h, and then a Bacillus velezensis bacterial solution is obtained; S1-4. The Bacillus subtilis bacterial solution and the Bacillus velezensis bacterial solution are mixed at a volume ratio of (1.8-2):1 to obtain a mixed bacterial solution, which is mixed with the solid-liquid mixture at a ratio of 1:10 mL / g, and then fermented at a rotation speed of 200-300 r / min at a temperature of 30±2°C with a ventilation volume of 1.5-2 L / min for 24 h. After the fermentation is completed, the residue is removed by centrifugation and filtration, and the supernatant is obtained. The supernatant is subjected to low-temperature instantaneous sterilization treatment, and then the supernatant is subjected to spray drying to obtain the Radix Polygoni Multiflori extract; The viable cell count of the Bacillus subtilis liquid is ≥10 6 CFU / mL, Bacillus subtilis was purchased from Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.1.131; The viable cell count of the bacillus velezensis bacterial liquid is ≥10 6 CFU / mL, bacillus velezensis is purchased from Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.1.

135.

2. The anti-dandruff composition according to claim 1, wherein The seed culture medium is prepared by mixing 5.0 g / L of proteose peptone, 3.0 g / L of beef extract, 5.0 g / L of NaCl, 0.005 g / L of MnSO4·H2O and 1 L of sterile water, and then sterilized at 121°C for 15 min.

3. The anti-dandruff composition according to claim 1, wherein In the step S1-1, the crushed Radix Polygoni Multiflori is sieved through a 60-100 mesh sieve.

4. The anti-dandruff composition according to claim 1, wherein In the step S1-4, the residue is removed by centrifugation at 8000 r / min for 25 min after the fermentation is completed.

5. Use of the anti-hair loss, oil control and dandruff removal composition according to any one of claims 1-4 in the preparation of a hair care product with the functions of anti-hair loss, oil control and dandruff removal.

6. An anti-shedding, oil-control, dandruff-controlling shampoo, characterized by, The shampoo comprises the anti-hair loss, oil control and dandruff removal composition according to any one of claims 1-4; The anti-hair loss, oil control and dandruff removal composition is added to the shampoo in an amount of 0.5-2 wt%; The shampoo further comprises a surfactant, a conditioning agent, a preservative, a humectant, a fragrance, an anti-dandruff agent, a thickening agent, a pH regulator, a colorant, a cooling agent and a solvent.

7. The anti-shedding, oil-controlling, dandruff-controlling shampoo according to claim 6, characterized in that, The surfactant is at least one of sodium laureth sulfate, cocamidopropyl betaine, sodium lauryl sulfate, cocamide MEA, quaternary ammonium salt-73, polysorbate-20, polyquaternium-52, laureth-16, dodecylbenzenesulfonic acid TEA salt, guar hydroxypropyltrimonium chloride and C12-15 pareth-3; The conditioning agent is at least one of trihydroxystearin, hydrolyzed chitin, hydrolyzed corn starch and dimethiconol; at least one of the preservatives is hydroxyacetophenone, phenoxyethanol, sodium benzoate; at least one of the humectants is 1,2-hexanediol, butylene glycol, glycerin, caprylyl glycol, propylene glycol, coconut oil; the fragrance is a daily fragrance; the antidandruff agent is piroctone olamine; at least one of the thickening agents is sodium chloride, acrylate / acrylamide copolymer; at least one of the pH regulators is citric acid, ethanolamine; at least one of the colorants is CI 15985, CI 16255, CI 42090; the cooling agent is menthol; the solvent is deionized water.

8. The method of making an anti-dandruff, control oil, and dandruff shampoo according to claim 6 or 7, characterized in that, The method comprises the following steps: stirring and mixing the anti-dandruff and oil-control composition, the surfactant, the conditioning agent, the preservative, the humectant, the fragrance, the antidandruff agent, the thickening agent, the pH regulator, the colorant, the cooling agent, and the solvent to obtain an anti-dandruff and oil-control shampoo.

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