Immunofluorescence analysis and detection device

Abstract

The invention discloses an immunofluorescence analysis and detection device, and relates to the technical field of detection instruments. According to the device, a plurality of containing discs containing first reactants are put into a conveying channel, and a first conveying assembly sequentially conveys the containing discs sliding out of the conveying channel to a first station; then a plurality of test tubes containing second reactants are placed on the mounting frame from the discharging opening, the rotating assembly drives the mounting frame to rotate, when one test tube is moved to the discharging opening, the test tube is separated from the mounting groove and penetrates through the discharging opening to abut against the pouring assembly, and the pouring assembly drives the test tube to incline so that the second reactants can be poured into the containing disc at the first station; the first reactant and the second reactant are mixed to form a to-be-detected object, at the moment, the first conveying assembly conveys the to-be-detected object to the second station, and then the second conveying assembly conveys the to-be-detected object to the detector for detection. The immunofluorescence analysis and detection device mixes a batch of first reactant and a batch of second reactant and then sends the mixture to the detector for detection, and the detection efficiency is high.

Description

Technical Field

[0001] The present invention relates to the technical field of detection instruments, and in particular to an immunofluorescence analysis detection device. Background Art

[0002] Immunofluorescence testing is to connect fluorescein with antibodies to form fluorescent antibodies, which then react with antigens in the sample to be tested, and place the reactants under a fluorescent microscope to observe the fluorescence emitted by the antigen-antibody complex, thereby identifying and locating the antigen in the sample. In the prior art, when testing the sample to be tested, usually only a single sample can be tested, and the detection efficiency is low. Summary of the invention

[0003] The main purpose of the present invention is to provide an immunofluorescence analysis detection device, aiming to solve the technical problem that the prior art can only detect a single sample and has low detection efficiency.

[0004] To achieve the above object, the present invention provides an immunofluorescence analysis detection device, comprising:

[0005] A plurality of holding trays, wherein the holding trays are used to hold the first reactant;

[0006] A housing, wherein the housing is provided with a conveying channel for conveying a plurality of the containing trays;

[0007] A detector, used for detecting an object to be detected;

[0008] The placement assembly comprises a mounting tube and a mounting frame; the mounting tube is tiltedly arranged on the shell, and the axial end and the side wall of the mounting tube are respectively provided with a discharge port and a discharge port; the mounting frame is rotatably arranged in the mounting tube, and the mounting frame is circumferentially provided with N mounting grooves for mounting test tubes, and the test tubes can be detached from the mounting grooves and pass through the discharge port; the test tubes contain a second reactant;

[0009] A rotating assembly, used to drive the mounting frame to rotate around the mounting cylinder;

[0010] A first conveying assembly is located below the conveying channel, and is used to drive the containing tray to pass through the first station and the second station in sequence;

[0011] A pouring assembly, used for tilting the test tube located at the discharge port to pour out the second reactant, the second reactant enters the holding tray at the first station, and the first reactant and the second reactant are mixed to form an object to be detected;

[0012] The second conveying assembly is used to convey the placing tray located at the second station to below the detector.

[0013] Optionally, the rotating assembly includes a first shaft, a universal coupling and a second shaft connected in sequence; the first shaft passes through the mounting tube and is connected to the mounting frame, and the first shaft is rotationally connected to the mounting tube; the second shaft is rotationally connected to the shell.

[0014] Optionally, it further includes a first transmission assembly; the first conveying assembly includes a first turntable, a second turntable and an intermittent drive unit;

[0015] The first turntable is rotatably connected to the housing, and the first turntable is located above the second turntable; the first turntable is circumferentially provided with N first holes for passing the holding tray, the second turntable is provided with a second hole corresponding to the second station, and the second hole is located above the second conveying assembly; after the first turntable rotates, the first hole can move to the bottom of the conveying channel, the first station and the second station;

[0016] The intermittent driving unit is used to drive the first turntable to rotate, and each rotation angle is 360 / N degrees; the input end and the output end of the first transmission component are respectively connected to the intermittent driving unit and the rotating component.

[0017] Optionally, the intermittent driving unit includes a dial, a toggle member and a first motor;

[0018] The first motor is arranged on the shell and is used to drive the toggle member to rotate; the toggle member is in the shape of a disc and is provided with a fork, and the fork is located on the side wall of the toggle member; the dial is coaxially connected to the rotating shaft of the first rotating disk, and the dial is circumferentially provided with N grooves cooperating with the fork and N arc-shaped notches, and the arc-shaped notch is located between two adjacent grooves, and the arc-shaped notch cooperates with the side wall of the toggle member.

[0019] Optionally, the first transmission assembly includes a first wheel, a second wheel and a first transmission member; the first wheel is connected to the rotating shaft of the first turntable; the second wheel is rotationally connected to the shell, and the second wheel is transmission-connected to the rotating assembly; the first transmission member is used to transmit the power of the first wheel to the second wheel.

[0020] Optionally, it also includes a second transmission assembly; the input end and the output end of the second transmission assembly are respectively connected to the tipping assembly and the second turntable.

[0021] Optionally, the dumping assembly includes a baffle, a first gear, a second gear and a second motor; the baffle is rotatably connected to the mounting cylinder, and the baffle is used to seal the discharge port; the baffle is provided with the second gear; the second motor is arranged on the shell and is used to drive the first gear to rotate, and the first gear cooperates with the second gear.

[0022] Optionally, the baffle is provided with a retaining ring located at the mouth of the test tube, and the retaining ring is used to prevent the test tube from detaching from the inclined baffle; the retaining ring is provided with a flow groove for the second reactant to pass through.

[0023] Optionally, the second transmission assembly includes a third wheel, a fourth wheel, a fifth wheel, a sixth wheel, a second transmission member, a third transmission member, a first bevel gear and a second bevel gear;

[0024] The third wheel is arranged on the first gear; the fourth wheel is rotatably connected to the housing; the second transmission member is used to transmit the power of the third wheel to the fourth wheel; the fourth wheel is provided with the first bevel gear; the second bevel gear is rotatably connected to the housing, and the rotating shaft of the second bevel gear is coaxially connected to the fifth wheel; the first bevel gear is meshed with the second bevel gear;

[0025] Among them, the first turntable is provided with a rotating shaft rotatably connected to the shell as a connecting shaft; the second turntable is provided with a sleeve sleeved on the outside of the connecting shaft; the sixth wheel is sleeved on the outside of the connecting shaft and axially connected to the sleeve; the third transmission member is used to transmit the power of the fifth wheel to the sixth wheel.

[0026] Optionally, the second conveying assembly includes a slide plate, a third motor, a driving roller, a transmission roller and a conveyor belt;

[0027] The third motor is arranged on the shell and is used to drive the driving roller to rotate; the transmission roller is rotationally connected to the shell; the conveyor belt is transmission-connected to the driving roller and the transmission roller; the slide plate is arranged on the shell, the upper end of the slide plate is located below the second workstation, and the lower end of the slide plate is located above the conveying start of the conveyor belt; the detector is located above the conveyor belt.

[0028] The technical solution of the present invention proposes an immunofluorescence analysis detection device, wherein a plurality of trays containing a first reactant are placed into a conveying channel, and a first conveying component sequentially conveys the trays that slide out of the conveying channel to a first station; then a plurality of test tubes containing a second reactant are placed from a discharge port on a mounting frame in a mounting cylinder, and a rotating component drives the mounting frame to rotate. When a test tube is moved to a discharge port, the test tube is separated from the mounting groove and passes through abutting dumping components from the discharge port, and the dumping components drive the test tube to tilt so as to pour the second reactant into the tray at the first station, and the first reactant and the second reactant are mixed in the tray to form an object to be detected. At this time, the first conveying component then conveys the tray containing the object to be detected to a second station, and the second conveying component then conveys the tray containing the object to be detected to a detector for detection. During the entire detection process, the immunofluorescence analysis detection device mixes a batch of the first reactant and the second reactant and then delivers them to the detector for detection, and the detection efficiency is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0030] Figure 1 A cross-sectional view of an immunofluorescence analysis detection device according to an embodiment of the present invention;

[0031] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0032] Figure 3 Another cross-sectional view of the immunofluorescence analysis detection device according to an embodiment of the present invention;

[0033] Figure 4 It is a structural schematic diagram of placing components according to an embodiment of the present invention;

[0034] Figure 5 It is an exploded view of the first conveying assembly according to an embodiment of the present invention.

[0035] Icons: 10, holding plate; 20, shell; 201, accommodating chamber; 202, mounting hole; 210, conveying channel; 30, detector; 410, mounting cylinder; 4101, discharge port; 4102, discharge port; 420, mounting frame; 4201, mounting slot; 501, first station; 502, second station; 510, first turntable; 5101, first hole; 511, connecting shaft; 520, second turntable; 5201, second hole; 521, sleeve; 5310, dial; 53101, slot; 53102, arc-shaped notch; 5320, toggle member; 5321, fork; 5330, first motor; 61 0, first wheel; 620, second wheel; 630, first transmission member; 710, first shaft; 720, universal coupling; 730, second shaft; 810, baffle; 820, first gear; 830, second gear; 840, second motor; 840, retaining ring; 8401, flow groove; 910, third wheel; 920, fourth wheel; 930, fifth wheel; 940, sixth wheel; 950, second transmission member; 960, third transmission member; 970, first bevel gear; 980, second bevel gear; 1010, skateboard; 1020, third motor; 1030, drive roller; 1040, transmission roller; 1050, conveyor belt. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0038] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0039] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0040] refer to Figure 1-Figure 5 , an immunofluorescence analysis detection device, comprising: a plurality of holding trays 10, the holding trays 10 are used to hold a first reactant; a housing 20, the housing 20 is provided with a conveying channel 210 for conveying the plurality of holding trays 10; a detector 30, used to detect an object to be detected; a placement component, comprising a mounting cylinder 410 and a mounting frame 420; the mounting cylinder 410 is tiltedly arranged on the housing 20, and the axial end and side wall of the mounting cylinder 410 are respectively provided with a discharge port 4101 and a discharge port 4102; the mounting frame 420 is rotatably arranged in the mounting cylinder 410, and the mounting frame 420 is circumferentially provided with N mounting grooves 4201 for mounting test tubes, and the test tubes can be removed from the mounting grooves 420 1 is separated from and passes through the discharge port 4102; the test tube contains the second reactant; the rotating assembly is used to drive the mounting frame 420 to rotate around the mounting cylinder 410; the first conveying assembly is located below the conveying channel 210, and the first conveying assembly is used to drive the holding tray 10 to pass through the first station 501 and the second station 502 in sequence; the pouring assembly is used to tilt the test tube located at the discharge port 4102 to pour out the second reactant, and the second reactant enters the holding tray 10 at the first station 501, and the first reactant and the second reactant are mixed to form a to-be-detected object; the second conveying assembly is used to convey the holding tray 10 located at the second station 502 to the bottom of the detector 30.

[0041] A plurality of trays 10 containing a first reactant are placed into the conveying channel 210, and the first conveying assembly conveys the trays 10 that slide out of the conveying channel 210 to the first station 501 in turn; then a plurality of test tubes containing a second reactant are placed from the discharge port 4101 on the mounting rack 420 in the mounting cylinder 410, and the rotating assembly drives the mounting rack 420 to rotate. When a test tube is moved to the discharge port 4102, the test tube is separated from the mounting groove 4201 and passes through the abutting pouring assembly from the discharge port 4102, and the pouring assembly drives the test tube to tilt so as to pour the second reactant into the tray 10 at the first station 501, and the first reactant and the second reactant are mixed in the tray 10 to form an object to be detected. At this time, the first conveying assembly conveys the tray 10 containing the object to be detected to the second station 502, and the second conveying assembly conveys the tray 10 containing the object to be detected to the detector 30 for detection. During the entire detection process, the immunofluorescence analysis detection device mixes the batches of first reactants and second reactants and then sends them to the detector 30 for detection, so the detection efficiency is high.

[0042] The housing 20 may be provided with a receiving cavity 201, and the housing 20 may be provided with a mounting hole 202, so that the mounting cylinder 410 can be placed into the receiving cavity 201 through the mounting hole 202. The housing 20 may be provided with a bracket for fixing the mounting cylinder 410.

[0043] The conveying channel 210 may be vertically disposed on the housing 20 , the inlet of the conveying channel 210 may be located at the upper end of the housing 20 , and the lower end of the conveying channel 210 is located above the first conveying assembly.

[0044] In some embodiments, a first transmission assembly is further included; the first conveying assembly includes a first turntable 510, a second turntable 520 and an intermittent drive unit;

[0045] The first turntable 510 is rotatably connected to the housing 20, and the first turntable 510 is located above the second turntable 520; the first turntable 510 is circumferentially provided with N first holes 5101 for passing the holding tray 10, and the second turntable 520 is provided with a second hole 5201 corresponding to the second station 502, and the second hole 5201 is located above the second conveying assembly; after the first turntable 510 rotates, the first hole 5101 can move to the bottom of the conveying channel 210, the first station 501 and the second station 502;

[0046] The intermittent driving unit is used to drive the first turntable 510 to rotate, and each rotation angle is 360 / N degrees; the input end and the output end of the first transmission component are respectively connected to the intermittent driving unit and the rotating component.

[0047] After multiple trays 10 are placed in the conveying channel 210, they are stacked up and down. A portion of the tray 10 at the bottom extends out of the conveying channel 210 and abuts against the first turntable 510, while the other portion of the tray 10 is located in the conveying channel 210. At this time, the first turntable 510 cannot drive the tray 10 to rotate. When the first turntable 510 rotates so that the first hole 5101 is located below the conveying channel 210, the tray 10 enters the first hole 5101 and abuts against the second turntable 520. At this time, the upper end of the tray 10 located in the first hole 5101 is located below the lower end of the conveying channel 210, and the thickness of the first turntable 510 is less than the height of the tray 10.

[0048] The intermittent drive unit drives the first rotating disk 510 to rotate intermittently, and each rotation angle is 360 / N degrees. Since the input end and the output end of the first transmission assembly are respectively connected to the intermittent drive unit and the rotating assembly, the rotating assembly also rotates intermittently and the rotation angle is 360 / N degrees. For example, N can be 8. At this time, the rotation angle of the first rotating disk 510 and the rotating assembly is 45 degrees, and the first rotating disk 510 can be provided with 8 first holes 5101.

[0049] When the first turntable 510 drives the holding tray 10 to rotate to the first station 501, the intermittent drive unit can temporarily stop working. At this time, the first turntable 510 and the mounting frame 420 both stop rotating, and the tipping assembly can drive the test tube to tilt. It should be noted that the number of the second hole 5201 is one, and the holding tray 10 will not pass through the second hole 5201 when moving from the conveying channel 210 to the first station 501.

[0050] When the first turntable 510 rotates forward to drive the tray 10 to move from the first station 501 to the second station 502, the tray 10 will not pass through the second hole 5201. When the first hole 5101 and the second hole 5201 are aligned at the second station 502, the tray 10 passes through the first hole 5101 and the second hole 5201 and falls onto the second conveying assembly, and the second conveying assembly drives the tray 10 to move to the position of the detector 30. In some embodiments, the initial position of the second hole 5201 can be located at the second station 502. In other embodiments, the path for the first hole 5101 to move from the position of the conveying channel 210 to the second station 502 is a first arc, the path for the first hole 5101 to move from the second station 502 to the conveying channel 210 is a second arc, the initial position of the second hole 5201 can be located on the second arc, and when the placing tray 10 moves to the second station 502, the intermittent drive unit temporarily stops working, and the second turntable 520 can rotate in reverse to move the second hole 5201 to the second station 502.

[0051] In some embodiments, the intermittent drive unit includes a dial 5310, a toggle member 5320 and a first motor 5330; the first motor 5330 is arranged on the housing 20 and is used to drive the toggle member 5320 to rotate; the toggle member 5320 is in the shape of a disc and is provided with a fork 5321, and the fork 5321 is located on the side wall of the toggle member 5320; the dial 5310 is coaxially connected to the rotating shaft of the first turntable 510, and the dial 5310 is circumferentially provided with N slots 53101 cooperating with the fork 5321 and N arc-shaped notches 53102, and the arc-shaped notch 53102 is located between two adjacent slots 53101, and the arc-shaped notch 53102 cooperates with the side wall of the toggle member 5320.

[0052] The first motor 5330 drives the toggle member 5320 to rotate. The toggle member 5320 rotates one circle to drive the shift fork 5321 to cooperate with one of the slots 53101 of the dial 5310. The shift fork 5321 drives the dial 5310 to rotate 360 / N degrees.

[0053] In some embodiments, the first transmission assembly includes a first wheel 610, a second wheel 620 and a first transmission member 630; the first wheel 610 is connected to the rotating shaft of the first turntable 510; the second wheel 620 is rotationally connected to the shell 20, and the second wheel 620 is transmission-connected to the rotating assembly; the first transmission member 630 is used to transmit the power of the first wheel 610 to the second wheel 620.

[0054] When the intermittent driving unit drives the first rotating disk 510 to rotate, the first wheel 610 intermittently rotates at the same angle as the first rotating disk 510. The first wheel 610 and the second wheel 620 may be pulleys, and the first transmission member 630 may be a belt.

[0055] In some embodiments, the rotating assembly includes a first shaft 710, a universal joint 720 and a second shaft 730 connected in sequence; the first shaft 710 passes through the mounting tube 410 and is connected to the mounting frame 420, and the first shaft 710 is rotationally connected to the mounting tube 410; the second shaft 730 is rotationally connected to the shell 20.

[0056] The second shaft 730 can be vertically disposed on the housing 20 and rotatably connected to the housing 20 . The second wheel 620 drives the second shaft 730 to rotate, and the second shaft 730 drives the first shaft 710 to rotate via the universal coupling 720 .

[0057] In some embodiments, a second transmission assembly is further included; the input end and the output end of the second transmission assembly are respectively connected to the dumping assembly and the second turntable 520.

[0058] The path of the first hole 5101 moving from the position of the conveying channel 210 to the second station 502 is a first arc, the path of the first hole 5101 moving from the second station 502 to the conveying channel 210 is a second arc, and the initial position of the second hole 5201 can be located on the second arc. When the tipping assembly drives the test tube to tilt, the second transmission assembly drives the second turntable 520 to rotate, so that the second hole 5201 moves to the second station 502.

[0059] In some embodiments, the dumping assembly includes a baffle 810, a first gear 820, a second gear 830 and a second motor 840; the baffle 810 is rotatably connected to the mounting cylinder 410, and the baffle 810 is used to seal the discharge port 4102; the baffle 810 is provided with a second gear 830; the second motor 840 is arranged on the shell 20 and is used to drive the first gear 820 to rotate, and the first gear 820 cooperates with the second gear 830.

[0060] The second motor 840 drives the baffle 810 to rotate around the mounting tube 410 through the first gear 820 and the second gear 830. During the tilting process of the baffle 810, the test tube fits against the baffle 810 under the action of gravity. The surface of the baffle 810 close to the test tube is an arc-shaped surface, so that the test tube and the baffle 810 fit closely, thereby preventing the test tube from shaking during the tilting process of the baffle 810.

[0061] In some embodiments, the baffle 810 is provided with a retaining ring 840 located at the test tube opening, and the retaining ring 840 is used to prevent the test tube from detaching from the tilted baffle 810; the retaining ring 840 is provided with a flow slot 8401 for the second reactant to pass through. After the baffle 810 is tilted, the test tube opening abuts the retaining ring 840, and at this time, the second reactant flows from the test tube opening through the flow slot 8401 to the holding tray 10 at the first station 501.

[0062] In some embodiments, the second transmission assembly includes a third wheel 910, a fourth wheel 920, a fifth wheel 930, a sixth wheel 940, a second transmission member 950, a third transmission member 960, a first bevel gear 970 and a second bevel gear 980;

[0063] The third wheel 910 is arranged on the first gear 820; the fourth wheel 920 is rotatably connected to the housing 20; the second transmission member 950 is used to transmit the power of the third wheel 910 to the fourth wheel 920; the fourth wheel 920 is provided with a first bevel gear 970; the second bevel gear 980 is rotatably connected to the housing 20, and the rotating shaft of the second bevel gear 980 is coaxially connected to the fifth wheel 930; the first bevel gear 970 is meshed with the second bevel gear 980;

[0064] Among them, the first turntable 510 is provided with a rotating shaft rotatably connected to the shell 20 as a connecting shaft 511; the second turntable 520 is provided with a sleeve 521 sleeved on the outside of the connecting shaft 511; the sixth wheel 940 is sleeved on the outside of the connecting shaft 511 and is axially connected to the sleeve 521; the third transmission member 960 is used to transmit the power of the fifth wheel 930 to the sixth wheel 940.

[0065] The first motor 5330 drives the first gear 820 to rotate, the third wheel 910 rotates with the first gear 820, the second transmission member 950 transmits the power of the third wheel 910 to the fourth wheel 920, the first bevel gear 970 rotates with the fourth wheel 920, the first bevel gear 970 drives the fifth wheel 930 to rotate through the second bevel gear 980, and the third transmission member 960 transmits the power of the fifth wheel 930 to the sixth wheel 940, thereby transmitting the power of the dumping assembly to the second turntable 520.

[0066] In some embodiments, the second conveying assembly includes a slide plate 1010, a third motor 1020, a driving roller 1030, a transmission roller 1040 and a conveyor belt 1050;

[0067] The third motor 1020 is arranged on the shell 20 and is used to drive the driving roller 1030 to rotate; the transmission roller 1040 is rotationally connected to the shell 20; the conveyor belt 1050 is transmission-connected to the driving roller 1030 and the transmission roller 1040; the slide plate 1010 is arranged on the shell 20, the upper end of the slide plate 1010 is located below the second workstation 502, and the lower end of the slide plate 1010 is located above the conveying start of the conveyor belt 1050; the detector 30 is located above the conveyor belt 1050.

[0068] The tray 10 at the second station 502 falls from the second hole 5201 onto the slide 1010, and slides from the slide 1010 onto the conveyor belt 1050. The third motor 1020 drives the driving roller 1030 to rotate, and the driving roller 1030 drives the conveyor belt 1050 to rotate. The conveyor belt 1050 transports the tray 10 containing the object to be detected to the bottom of the detector 30 so that the detector 30 can detect it.

[0069] The above descriptions are only optional embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. An immunofluorescence analysis detection device, characterized in that: include: A plurality of holding trays, wherein the holding trays are used to hold the first reactant; A housing, wherein the housing is provided with a conveying channel for conveying a plurality of the containing trays; A detector, used for detecting an object to be detected; The placement assembly comprises a mounting tube and a mounting frame; the mounting tube is tiltedly arranged on the shell, and the axial end and the side wall of the mounting tube are respectively provided with a discharge port and a discharge port; the mounting frame is rotatably arranged in the mounting tube, and the mounting frame is circumferentially provided with N mounting grooves for mounting test tubes, and the test tubes can be detached from the mounting grooves and pass through the discharge port; the test tubes contain a second reactant; A rotating assembly, used to drive the mounting frame to rotate around the mounting cylinder; A first conveying assembly is located below the conveying channel, and is used to drive the containing tray to pass through the first station and the second station in sequence; A pouring assembly, used for tilting the test tube located at the discharge port to pour out the second reactant, the second reactant enters the holding tray at the first station, and the first reactant and the second reactant are mixed to form an object to be detected; The second conveying assembly is used to convey the placing tray located at the second station to below the detector.

2. The immunofluorescence analysis detection device according to claim 1, characterized in that: The rotating assembly includes a first shaft, a universal coupling and a second shaft which are connected in sequence; the first shaft passes through the mounting tube and is connected to the mounting frame, and the first shaft is rotationally connected to the mounting tube; the second shaft is rotationally connected to the housing.

3. The immunofluorescence analysis detection device according to claim 1, characterized in that: It also includes a first transmission assembly; the first conveying assembly includes a first turntable, a second turntable and an intermittent drive unit; The first turntable is rotatably connected to the housing, and the first turntable is located above the second turntable; the first turntable is circumferentially provided with N first holes for passing the holding tray, the second turntable is provided with a second hole corresponding to the second station, and the second hole is located above the second conveying assembly; after the first turntable rotates, the first hole can move to the bottom of the conveying channel, the first station and the second station; The intermittent driving unit is used to drive the first turntable to rotate, and each rotation angle is 360 / N degrees; the input end and the output end of the first transmission component are respectively connected to the intermittent driving unit and the rotating component.

4. The immunofluorescence analysis detection device according to claim 3, characterized in that: The intermittent driving unit comprises a dial, a toggle member and a first motor; The first motor is arranged on the shell and is used to drive the toggle member to rotate; the toggle member is in the shape of a disc and is provided with a fork, and the fork is located on the side wall of the toggle member; the dial is coaxially connected to the rotating shaft of the first rotating disk, and the dial is circumferentially provided with N grooves cooperating with the fork and N arc-shaped notches, and the arc-shaped notch is located between two adjacent grooves, and the arc-shaped notch cooperates with the side wall of the toggle member.

5. The immunofluorescence analysis detection device according to claim 3, characterized in that: The first transmission assembly includes a first wheel, a second wheel and a first transmission member; the first wheel is connected to the rotating shaft of the first turntable; the second wheel is rotationally connected to the shell, and the second wheel is transmission-connected to the rotating assembly; the first transmission member is used to transmit the power of the first wheel to the second wheel.

6. The immunofluorescence analysis detection device according to claim 3, characterized in that: It also includes a second transmission assembly; the input end and the output end of the second transmission assembly are respectively connected to the tipping assembly and the second turntable.

7. The immunofluorescence analysis detection device according to claim 6, characterized in that: The dumping assembly includes a baffle, a first gear, a second gear and a second motor; the baffle is rotatably connected to the mounting cylinder, and the baffle is used to seal the discharge port; the baffle is provided with the second gear; the second motor is arranged on the shell and is used to drive the first gear to rotate, and the first gear cooperates with the second gear.

8. The immunofluorescence analysis detection device according to claim 7, characterized in that: The baffle is provided with a baffle ring located at the test tube mouth, and the baffle ring is used to prevent the test tube from detaching from the inclined baffle; the baffle ring is provided with a flow groove for the second reactant to pass through.

9. The immunofluorescence analysis detection device according to claim 7, characterized in that: The second transmission assembly includes a third wheel, a fourth wheel, a fifth wheel, a sixth wheel, a second transmission member, a third transmission member, a first bevel gear and a second bevel gear; The third wheel is arranged on the first gear; the fourth wheel is rotatably connected to the housing; the second transmission member is used to transmit the power of the third wheel to the fourth wheel; the fourth wheel is provided with the first bevel gear; the second bevel gear is rotatably connected to the housing, and the rotating shaft of the second bevel gear is coaxially connected to the fifth wheel; the first bevel gear is meshed with the second bevel gear; Among them, the first turntable is provided with a rotating shaft rotatably connected to the shell as a connecting shaft; the second turntable is provided with a sleeve sleeved on the outside of the connecting shaft; the sixth wheel is sleeved on the outside of the connecting shaft and axially connected to the sleeve; the third transmission member is used to transmit the power of the fifth wheel to the sixth wheel.

10. The immunofluorescence analysis detection device according to claim 1, characterized in that: The second conveying assembly includes a slide plate, a third motor, a driving roller, a transmission roller and a conveyor belt; The third motor is arranged on the shell and is used to drive the driving roller to rotate; the transmission roller is rotationally connected to the shell; the conveyor belt is transmission-connected to the driving roller and the transmission roller; the slide plate is arranged on the shell, the upper end of the slide plate is located below the second workstation, and the lower end of the slide plate is located above the conveying start of the conveyor belt; the detector is located above the conveyor belt.

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