An automated microbial separation and cultivation device

Abstract

The present invention relates to the technical field of microbial isolation and cultivation, and particularly relates to a microbial automatic isolation and cultivation device, which includes a base and a centrifugal disk rotatably arranged on the base. A top cover detachably arranged on the upper end of the centrifugal disk is hermetically matched with the centrifugal disk. A circulation pump is arranged on the top cover. During use, a precipitation bottom tank and a storage cylinder are driven by a second driving component to flip synchronously in positive and negative directions, so that their center lines are kept horizontal or vertical states, and cooperate with a first driving component to drive the centrifugal disk to rotate to complete the centrifugation operation. The formed precipitated microorganisms are placed in a precipitation bottom tank matched with the large-diameter port of the storage cylinder, and the supernatant is placed in the storage cylinder, thereby preventing the precipitated microorganisms from flowing back into the supernatant again, ensuring the separation effect, and automatically discharging the precipitated microorganisms into the culture medium for cultivation through a material taking component, realizing the provision of cultivation conditions suitable for different types of microorganisms, and having a wide range of applications.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial isolation and cultivation, and particularly to a microbial automatic isolation and cultivation device. Background Art

[0002] The cultivation step in pharmaceutical microbial detection and analysis is an important link to ensure the accuracy and reliability of the detection results. Usually, for a solution containing pharmaceutical microorganisms, direct filtration or centrifugal precipitation is used to complete the microbial separation, and then the separated microorganisms are placed on a culture medium suitable for microbial growth for cultivation. The prior art uses a more efficient centrifugal precipitation method in combination with a corresponding separation device to achieve rapid microbial separation.

[0003] For example, the Chinese patent with the application number CN202410330384.7 discloses a microbial separation device for pharmaceutical microbial inspection. The separation device body includes a centrifugal mechanism, a separation component, and a dialysis component. By installing multiple dialysis components at the edge of the centrifugal mechanism in an inclined state, the preliminary separation process of microbial precipitation and supernatant can be completed during centrifugation. Combining with the subsequent lifting movement control of the separation component, the purpose of rapid separation and efficient repeated separation can be achieved, making the microbial separation more thorough. However, in this separation device, after the microbial base liquid is centrifuged inside the centrifugal channel, the precipitated microorganisms accumulated on the surface of the dialysis component will flow back into the supernatant again in the state of stopping centrifugation, and the separation effect is not ideal. Moreover, after the microbial precipitate enters the collection bin through the annular docking port, the docking bin needs to be opened to take out the microbial precipitate. However, for anaerobic microorganisms, being exposed to the air environment during the separation and cultivation process will cause the death of microorganisms or the inhibition of their growth, resulting in limited application scope of this separation device. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a microbial automatic isolation and cultivation device to solve the technical problems in the prior art that the precipitated microorganisms will flow back into the supernatant again in the state of stopping centrifugation, the separation effect is not ideal, and the application scope is limited.

[0005] Based on the above purpose, the present invention provides a microbial automatic isolation and cultivation device, which includes a base and a centrifugal disk rotatably arranged on the base. A top cover detachably arranged on the upper end of the centrifugal disk is hermetically fitted with the centrifugal disk. A circulation pump is arranged on the top cover, and the circulation pump is connected to the closed inner cavity formed by the centrifugal disk and the top cover through a circulation pipeline. A plurality of culture media are circumferentially installed on the centrifugal disk. The automatic isolation and cultivation device further includes:

[0006] Storage cylinder, the storage cylinder is a conical cavity structure with both ends open, and there are several storage cylinders, and several storage cylinders can be rotatably arranged in a closed inner cavity;

[0007] Precipitation bottom tank, the large-diameter port of the storage cylinder is installed on the precipitation bottom tank;

[0008] Valve cover, the valve cover is installed at the small-diameter port of the storage cylinder;

[0009] Connecting pipe, one end of the connecting pipe is installed on the valve cover, the other end of the connecting pipe extends to the hemispherical bottom of the precipitation bottom tank, at least two discharge openings are provided on the hemispherical bottom of the precipitation bottom tank, and opening and closing parts are provided at the discharge openings on the inner wall of the hemispherical bottom of the precipitation bottom tank;

[0010] Feeding head matched with the discharge opening, the feeding head is controlled to open and close by an electromagnetic valve and is installed on the centrifugal disc through a feeding component, and is communicated with the culture medium through the feeding component;

[0011] Drive assembly one, the drive assembly one is used to drive the centrifugal disc to rotate to perform centrifugal separation on the microbial base liquid in several storage cylinders;

[0012] Drive assembly two, the drive assembly two is used to drive the storage cylinder to turn over so that its center line remains horizontal or vertical. When its center line is in the horizontal state, the feeding head is synchronously driven to extend into the precipitation bottom tank to suck the precipitated microorganisms after centrifugal separation, and is automatically discharged into the culture medium through the feeding component for cultivation. When its center line is in the vertical state, the connecting pipe is communicated with the flow pipeline.

[0013] Further, the drive assembly one includes:

[0014] Drive motor, the drive motor is installed on the base through a mounting frame;

[0015] Drive wheel, the drive wheel is installed on the output end of the drive motor;

[0016] Driven wheel, the driven wheel is rotatably installed on the base through a rotating shaft, the driven wheel meshes with the drive wheel, and the upper end of the rotating shaft is coaxially installed on the lower end surface of the centrifugal disc.

[0017] Further, the drive assembly two includes:

[0018] Cylinder, the cylinder is installed on the upper end surface of the centrifugal disc;

[0019] Lifting frame, the lifting frame is installed on the output end of the cylinder, and several support plates protrude from the side end of the lifting frame;

[0020] Fixed seat, the fixed seat is installed inside the centrifugal disk;

[0021] Support rod, the lower end of the support rod is installed on the support plate, and the support rod is also slidably arranged on the fixed seat;

[0022] Slider, the slider is hinged to the upper end of the support rod;

[0023] Support platform, the support platform is fixedly installed on the fixed seat;

[0024] Guide rod, one end of the guide rod is hinged to the support platform, the precipitation bottom tank is fixedly connected to this end of the guide rod, the other end of the guide rod is clamped on the valve cover, and the slider is also slidably arranged on the guide rod.

[0025] Further, the opening and closing member includes a reed and a closing piece. The reed is fixedly installed on the inner wall of the hemispherical bottom of the precipitation bottom tank, the closing piece is installed at the end of the reed, and the closing piece covers the discharge opening.

[0026] Further, the material taking assembly includes:

[0027] Diversion seat, the diversion seat is fixedly installed on the centrifugal disk, a centrifugal channel is arranged inside the diversion seat, an interface is arranged on the side end of the culture medium, and the interface is communicated with the centrifugal channel;

[0028] Turning arm, the material taking head is installed on the turning arm, the lower end of the turning arm is hinged to the diversion seat, the material taking head is communicated with the centrifugal channel through the cavity structure of the turning arm, and an inclined groove is arranged on the turning arm;

[0029] Slide rod, the slide rod is slidably arranged on the diversion seat, and the lower end of the slide rod is connected to the lifting frame;

[0030] Sliding head, the middle part of the sliding head is installed at the top end of the slide rod, and both ends of the sliding head are slidably arranged in the inclined groove;

[0031] Pressure pump, the pressure pump is installed at the upper end of the diversion seat, and the working end of the pressure pump is communicated with the centrifugal channel.

[0032] Further, there are at least two turning arms. After the ends of the two turning arms are turned close to each other, the material taking head extends into the precipitation bottom tank through the discharge opening, and the opening and closing member is in an open state.

[0033] Further, elastic clamping pieces are arranged at both ends of the precipitation bottom tank, and the clamping ends of the elastic clamping pieces are matched with the outer end surface of the storage cylinder.

[0034] Furthermore, a stabilizing portion for positioning the valve cover is also installed on the fixed seat, and the stabilizing portion includes:

[0035] A guide rod, which is installed on the fixed seat;

[0036] A mounting block, which is slidably arranged on the guide rod;

[0037] A spring, which is sleeved on the guide rod, one end of the spring abuts against the mounting block, and the other end of the spring abuts against the fixed seat;

[0038] A clamping plate, which is installed at the upper end of the mounting block, and the edge end of the valve cover is clamped on the clamping plate.

[0039] Furthermore, a plurality of positioning blocks are circumferentially installed on the inner wall of the top cover, positioning grooves are provided on each of the plurality of positioning blocks, and the plurality of positioning grooves are all matched with the outer side wall of the storage cylinder.

[0040] Furthermore, an avoidance groove is provided at the side end of the valve cover, and the depth of the opening of the avoidance groove is not less than the protruding length of the positioning groove.

[0041] Beneficial effects of the present invention: When using a microbial automatic separation and cultivation device of the present invention, the precipitation bottom tank and the storage cylinder are driven by the second driving component to synchronously flip in positive and negative directions, so that their center lines are kept horizontal or vertical, and in cooperation with the first driving component driving the centrifugal disk to rotate to complete the centrifugation operation. The formed precipitated microorganisms are placed in the precipitation bottom tank matched with the large-diameter port of the storage cylinder, and the supernatant is placed in the storage cylinder, thereby preventing the precipitated microorganisms from flowing back into the supernatant again, ensuring the separation effect, and automatically discharging the precipitated microorganisms into the culture medium for cultivation through the material taking component, realizing the provision of cultivation conditions suitable for different types of microorganisms, and having a wide range of applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only those of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.

[0043] Figure 1 It is a schematic diagram of the internal structure of the present invention;

[0044] Figure 2 It is a schematic diagram of the external structure of the present invention;

[0045] Figure 3This is an assembly schematic diagram of the storage cylinder and some structures in the horizontal state of the present invention;

[0046] Figure 4 This is an assembly schematic diagram of some internal structures in the second driving component of the present invention;

[0047] Figure 5 This is a structural schematic diagram of the precipitation bottom tank of the present invention;

[0048] Figure 6 This is an assembly schematic diagram of the material taking component of the present invention;

[0049] Figure 7 This is an internal structural schematic diagram of the material taking component of the present invention;

[0050] Figure 8 This is a state schematic diagram of the first driving component and the top cover of the present invention.

[0051] The markings in the figure are:

[0052] 1. Base; 2. Centrifugal disk; 3. Top cover; 4. Circulation pump; 5. Flow pipeline; 6. Culture medium;

[0053] 7. Storage cylinder; 8. Precipitation bottom tank; 9. Valve cover; 10. Connecting pipe; 11. Discharge opening; 12. Material taking head; 13. Driving motor; 14. Mounting frame; 15. Driving wheel; 16. Driven wheel; 17. Cylinder; 18. Lifting frame; 19. Fixed seat; 20. Support rod; 21. Slide block; 22. Support platform; 23. Guide rod; 24. Reed; 25. Sealing piece; 26. Shunt seat; 27. Centrifugal channel; 28. Docking port; 29. Flipping arm; 30. Inclined groove; 31. Slide bar; 32. Sliding head; 33. Pressure pump; 34. Elastic clamping piece; 35. Guide rod; 36. Mounting block; 37. Spring; 38. Clamping plate; 39. Positioning block; 40. Positioning groove; 41. Avoidance groove. Detailed implementation manners

[0054] In order to make the purpose, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to specific embodiments.

[0055] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present invention should have the ordinary meanings understood by those with ordinary skills in the field to which the present invention pertains. The "first", "second" and similar terms used in the present invention do not denote any order, quantity or importance, but are only used to distinguish different components. Words such as "comprising" or "including" mean that the elements or objects appearing before this word cover the elements or objects listed after this word and their equivalents, without excluding other elements or objects. Words such as "connected" or "coupled" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Upper", "lower", "left", "right", etc. are only used to represent relative position relationships, and when the absolute position of the object being described changes, the relative position relationship may also change accordingly.

[0056] In the first aspect of the present invention, a microbial automatic separation and cultivation device is proposed. As Figure 1-8 shown, it includes a base 1 and a centrifugal disk 2 rotatably arranged on the base 1. A top cover 3 detachably arranged on the upper end of the centrifugal disk 2 is in sealing cooperation with the centrifugal disk 2. A circulation pump 4 is arranged on the top cover 3, and the circulation pump 4 is connected through a circulation pipeline 5 to the closed inner cavity formed by the centrifugal disk 2 and the top cover 3. A number of culture media 6 are circumferentially installed on the centrifugal disk 2. The automatic separation and cultivation device further includes:

[0057] A storage cylinder 7, the storage cylinder 7 is a conical cavity structure with both ends open, and there are a number of storage cylinders 7, and a number of storage cylinders 7 can all be rotatably arranged in the closed inner cavity;

[0058] A precipitation bottom tank 8, the large-diameter port of the storage cylinder 7 is installed on the precipitation bottom tank 8;

[0059] A valve cover 9, the valve cover 9 is installed at the small-diameter port of the storage cylinder 7;

[0060] A connecting pipe 10, one end of the connecting pipe 10 is installed on the valve cover 9, the other end of the connecting pipe 10 extends to the hemispherical bottom of the precipitation bottom tank 8, at least two discharge openings 11 are opened on the hemispherical bottom of the precipitation bottom tank 8, and an opening and closing member is arranged on the inner wall of the hemispherical bottom of the precipitation bottom tank 8 at the discharge openings 11;

[0061] A material taking head 12 matching with the discharge opening 11, the material taking head 12 is controlled to open and close by an electromagnetic valve and is installed on the centrifugal disk 2 through a material taking assembly and is communicated with the culture medium 6 through the material taking assembly;

[0062] A driving component one, the driving component one is used to drive the centrifugal disk 2 to rotate to perform centrifugal separation on the microbial base liquid in a number of storage cylinders 7;

[0063] The second driving component is used to drive the storage cylinder 7 to turn over so that its center line remains horizontal or vertical. When its center line is in the horizontal state, the feeding head 12 is synchronously driven to extend into the sediment bottom tank 8 to suck the sediment microorganisms after centrifugal separation, and is automatically discharged into the culture medium 6 through the feeding component for cultivation. When its center line is in the vertical state, the connecting pipe 10 is communicated with the flow pipeline 5.

[0064] In this embodiment, the first driving component includes:

[0065] A driving motor 13, and the driving motor 13 is installed on the base 1 through a mounting bracket 14;

[0066] A driving wheel 15, and the driving wheel 15 is installed on the output end of the driving motor 13;

[0067] A driven wheel 16, and the driven wheel 16 is rotatably installed on the base 1 through a rotating shaft. The driven wheel 16 meshes with the driving wheel 15, and the upper end of the rotating shaft is coaxially installed on the lower end surface of the centrifugal disc 2.

[0068] In this embodiment, the second driving component includes:

[0069] A cylinder 17, and the cylinder 17 is installed on the upper end surface of the centrifugal disc 2;

[0070] A lifting frame 18, and the lifting frame 18 is installed on the output end of the cylinder 17. A plurality of support plates extend from the side end of the lifting frame 18;

[0071] A fixed seat 19, and the fixed seat 19 is installed inside the centrifugal disc 2;

[0072] A support rod 20, the lower end of the support rod 20 is installed on the support plate, and the support rod 20 also slides on the fixed seat 19;

[0073] A slider 21, and the slider 21 is hinged to the upper end of the support rod 20;

[0074] A support platform 22, and the support platform 22 is fixedly installed on the fixed seat 19;

[0075] A guide rod 23, one end of the guide rod 23 is hinged to the support platform 22, the sediment bottom tank 8 is fixedly connected to this end of the guide rod 23, the other end of the guide rod 23 is clamped on the valve cover 9, and the slider 21 also slides on the guide rod 23.

[0076] In this embodiment, the feeding component includes:

[0077] A flow dividing seat 26, the flow dividing seat 26 is fixedly installed on the centrifugal disc 2, a centrifugal channel 27 is arranged inside the flow dividing seat 26, an interface 28 is arranged on the side end of the culture medium 6, and the interface 28 is communicated with the centrifugal channel 27;

[0078] The turning arm 29 has a material taking head 12 mounted thereon. The lower end of the turning arm 29 is hinged to the shunt base 26. The material taking head 12 is connected to the centrifugal channel 27 through the cavity structure of the turning arm 29. An inclined groove 30 is provided on the turning arm 29;

[0079] The sliding rod 31 is slidably arranged on the shunt base 26, and the lower end of the sliding rod 31 is connected to the lifting frame 18;

[0080] The sliding head 32 has its middle part mounted on the top end of the sliding rod 31, and both ends of the sliding head 32 are slidably arranged in the inclined groove 30;

[0081] The pressure pump 33 is mounted on the upper end of the shunt base 26, and the working end of the pressure pump 33 is connected to the centrifugal channel 27.

[0082] In this embodiment, during use, the storage cylinder 7 is driven by the second driving component to turn upwards so that its center line is in a vertical state, that is, the output end of the cylinder 17 drives the lifting frame 18 to lift upwards, drives the slider 21 to slide on the guide rod 23 through the support rod 20, and then pushes the precipitation bottom tank 8 to turn around the hinge point with the support table 22, so that the storage cylinder 7 on the precipitation bottom tank 8 turns synchronously. Then the connecting pipe 10 is connected to the circulation pipeline 5, and the microbial base liquid to be separated is added into each storage cylinder 7 through the circulation pump 4, the circulation pipeline 5 and the connecting pipe 10. Under the closing action of the opening and closing member, the microbial base liquid will not flow out of the precipitation bottom tank 8;

[0083] After an appropriate amount of the microbial base liquid enters, the connecting pipe 10 is controlled by the valve cover 9 to be closed, and then the storage cylinder 7 is driven by the second driving component to turn downwards. Through the reverse execution of the above operations, the center line of the storage cylinder 7 is in a horizontal state. During this process, the lifting frame 18 will also drive the sliding head 32 to slide in the inclined groove 30 through the sliding rod 31 to pull the turning arm 29 to turn, thereby driving the material taking head 12 to extend into the discharge opening 11;

[0084] Then the first driving component is started, and the driving motor 13 drives the driving wheel 15 to rotate. Through the driven wheel 16 and the rotating shaft meshed with the driving motor 13, the centrifugal disc 2 is driven to rotate centrifugally. After the centrifugal operation is completed, the driving motor 13 is shut down, so that the centrifugal disc 2 gradually stops rotating. The precipitated microorganisms are placed in the precipitation bottom tank 8 matching the large-diameter port of the storage cylinder 7, and the supernatant is placed in the storage cylinder 7;

[0085] When detecting and analyzing anaerobic microorganisms, the solenoid valve on the material taking head 12 and the pressure pump 33 are simultaneously turned on. Under the negative pressure environment provided by the pressure pump 33, the air in the centrifugal channel 27 and the culture medium 6 is quickly extracted to form an anaerobic environment condition required by the microorganisms. The material taking head 12 sucks the precipitated microorganisms in the precipitation bottom tank 8, and enters the culture medium 6 through the cavity structure of the turning arm 29, the centrifugal channel 27 and the docking port 28. When detecting and analyzing aerobic microorganisms or insensitive microorganisms, the pressure pump 33 does not need to be turned on, and the precipitated microorganisms will flow by gravity along this flow path into the culture medium 6. The culture medium 6 contains coagulants such as agar, gelatin or carrageenan, etc. The microorganisms grow on the inner surface or inside of the culture medium 6 and finally grow into visible colonies to carry out operations such as microorganism detection analysis, identification and viable bacteria counting.

[0086] In this embodiment, as Figure 4 shown, the opening and closing member includes a reed 24 and a closing piece 25. The reed 24 is fixedly installed on the inner wall of the hemispherical bottom of the precipitation bottom tank 8, and the closing piece 25 is installed at the end of the reed 24. The closing piece 25 covers the discharge opening 11. When the material taking head 12 does not extend into the discharge opening 11, under the action of the self-elastic force of the reed 24, the closing piece 25 can completely block the discharge opening 11 to avoid liquid leakage.

[0087] In this embodiment, as Figure 3 、 Figure 6 shown, there are at least two turning arms 29. After the ends of the two turning arms 29 are turned close to each other, the material taking head 12 extends into the precipitation bottom tank 8 through the discharge opening 11, and the opening and closing member is in an open state, so that the material taking head 12 can completely suck the precipitated microorganisms in the precipitation bottom tank 8, avoiding the situation that the microorganisms on one side are insufficiently absorbed while the microorganisms on the other side accumulate.

[0088] In this embodiment, as Figure 3 、 Figure 4 、 Figure 5 shown, elastic clamping pieces 34 are provided at both ends of the precipitation bottom tank 8. The clamping ends of the elastic clamping pieces 34 cooperate with the outer end surface of the storage cylinder 7 to maintain the stability of the centrifugation process of the storage cylinder 7 and the precipitation bottom tank 8.

[0089] In this embodiment, as Figure 1 、 Figure 3 shown, a stabilizing portion for positioning the valve cover 9 is also installed on the fixed seat 19. The stabilizing portion includes:

[0090] A guide rod 35, the guide rod 35 is installed on the fixed seat 19;

[0091] A mounting block 36, the mounting block 36 is slidably arranged on the guide rod 35;

[0092] The spring 37 is sleeved on the guide rod 35. One end of the spring 37 abuts against the mounting block 36, and the other end of the spring 37 abuts against the fixed seat 19.

[0093] The clamping plate 38 is installed at the upper end of the mounting block 36. The edge end of the valve cover 9 is clamped on the clamping plate 38. During the centrifugation process, the valve cover 9 can remain stable on the clamping plate 38, avoiding the vibration of the storage cylinder 7 during the rotational centrifugation process, and ensuring that the position area of the precipitated microorganisms and the supernatant in the storage cylinder 7 is in a stable state.

[0094] In this embodiment, as Figure 1 , Figure 8 shown, a plurality of positioning blocks 39 are circumferentially installed on the inner wall of the top cover 3. Each of the plurality of positioning blocks 39 is provided with a positioning groove 40. The plurality of positioning grooves 40 are all matched with the outer side wall of the storage cylinder 7. The side end of the valve cover 9 is provided with an avoidance groove 41. The depth of the opening of the avoidance groove 41 is not less than the protruding length of the positioning groove 40. After the separation and cultivation operation is completed, a positive air pressure is provided to the microorganism flow path by the pressure pump 33 to automatically clean the flow path. Then, under the positioning of the positioning groove 40, the storage cylinder 7 is in a vertical state, the top cover 3 is opened, and the residual solution inside is sucked by the connecting pipe 10 to achieve the rapid cleaning of the entire separation and cultivation device.

[0095] Those of ordinary skill in the art should understand that: The discussion of any above embodiment is only exemplary, and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples; Under the concept of the present invention, the technical features in the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations in different aspects of the present invention as described above, and they are not provided in detail for the sake of brevity.

[0096] The present invention aims to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An automated microbial separation and cultivation device, comprising a base (1) and a centrifugal disc (2) rotatably mounted on the base (1), wherein a top cover (3) which is detachably mounted on the upper end of the centrifugal disc (2) and which is sealed and matched with the centrifugal disc (2), wherein a circulating pump (4) is mounted on the top cover (3), and the circulating pump (4) is connected to a closed inner cavity formed by the centrifugal disc (2) and the top cover (3) through a circulation pipe (5), wherein: A plurality of culture media (6) are annularly mounted on the centrifugal disk (2), and the automated separation and cultivation equipment further comprises: A storage cylinder (7), wherein the storage cylinder (7) is a conical cavity structure with openings at both ends, and there are a plurality of storage cylinders (7), and the plurality of storage cylinders (7) can be turned over and arranged in the closed inner cavity; A sedimentation bottom tank (8), on which the large-diameter port of the storage cylinder (7) is mounted; A valve cover (9), the valve cover (9) being installed at the small-diameter port of the storage cylinder (7); a connecting pipe (10), one end of the connecting pipe (10) being mounted on the valve cover (9), the other end of the connecting pipe (10) extending to the hemispherical bottom of the sedimentation trough (8), the hemispherical bottom of the sedimentation trough (8) being provided with at least two discharge openings (11), and an opening and closing member being provided on the inner wall of the hemispherical bottom of the sedimentation trough (8) at the discharge openings (11); a material taking head (12) matched with the discharge opening (11), the material taking head (12) is controlled to open and close by a solenoid valve and is installed on the centrifugal disk (2) through a material taking component, and is connected to the culture medium (6) through the material taking component; A driving component 1, the driving component 1 is used to drive the centrifugal disc (2) to rotate so as to centrifugally separate the microbial base liquid in the plurality of storage cylinders (7); A driving component 2, wherein the driving component 2 is used to drive the storage cylinder (7) to flip so that its center line remains in a horizontal or vertical state. When its center line is in a horizontal state, the material taking head (12) is synchronously driven to extend into the sedimentation bottom tank (8) to absorb the precipitated microorganisms after centrifugal separation, and automatically discharge them into the culture medium (6) for cultivation through the material taking component. When its center line is in a vertical state, the connecting pipe (10) is connected to the circulation pipe (5).

2. The microbial automated separation and cultivation equipment according to claim 1, characterized in that: The driving component 1 comprises: A driving motor (13), wherein the driving motor (13) is mounted on the base (1) via a mounting frame (14); A driving wheel (15), wherein the driving wheel (15) is mounted on the output end of the driving motor (13); A driven wheel (16) is rotatably mounted on the base (1) via a rotating shaft. The driven wheel (16) is meshed with the driving wheel (15). The upper end of the rotating shaft is coaxially mounted on the lower end surface of the centrifugal disc (2).

3. The microbial automated separation and cultivation equipment according to claim 1, characterized in that: The second driving component comprises: A cylinder (17), wherein the cylinder (17) is mounted on the upper end surface of the centrifugal disc (2); A lifting frame (18), the lifting frame (18) is installed on the output end of the cylinder (17), and a plurality of bracket plates extend from the side end of the lifting frame (18); A fixing seat (19), wherein the fixing seat (19) is installed inside the centrifugal disc (2); A support rod (20), the lower end of which is mounted on the bracket plate, and the support rod (20) is also slidably mounted on the fixing seat (19); A slider (21), wherein the slider (21) is hinged to the upper end of the support rod (20); A support platform (22), wherein the support platform (22) is fixedly mounted on the fixing seat (19); A guide rod (23), one end of the guide rod (23) is hinged on the support platform (22), the sedimentation trough (8) is fixedly connected to the end of the guide rod (23), the other end of the guide rod (23) is clamped on the valve cover (9), and the slider (21) is also slidably arranged on the guide rod (23).

4. The microbial automated separation and cultivation equipment according to claim 1, characterized in that: The opening and closing member comprises a reed (24) and a closing piece (25), wherein the reed (24) is fixedly mounted on the inner wall of the hemispherical bottom of the sedimentation trough (8), and the closing piece (25) is mounted on the end of the reed (24), and the closing piece (25) covers the discharge opening (11).

5. The microbial automated separation and cultivation equipment according to claim 3, characterized in that: The material taking component comprises: A flow diverter seat (26), the flow diverter seat (26) is fixedly mounted on the centrifugal disk (2), a centrifugal channel (27) is provided inside the flow diverter seat (26), a docking port (28) is provided at a side end of the culture medium (6), and the docking port (28) is in communication with the centrifugal channel (27); a flip arm (29), the material taking head (12) being mounted on the flip arm (29), the lower end of the flip arm (29) being hinged on the diverter seat (26), the material taking head (12) being connected to the centrifugal channel (27) through the cavity structure of the flip arm (29), and an inclined slot (30) being provided on the flip arm (29); A sliding rod (31), the sliding rod (31) is slidably disposed on the diverter seat (26), and the lower end of the sliding rod (31) is connected to the lifting frame (18); A sliding head (32), wherein the middle portion of the sliding head (32) is mounted on the top end of the sliding rod (31), and both ends of the sliding head (32) are slidably disposed in the inclined groove (30); A pressure pump (33), wherein the pressure pump (33) is installed at the upper end of the diverter seat (26), and the working end of the pressure pump (33) is connected to the centrifugal channel (27).

6. The microorganism automated separation and cultivation equipment according to claim 5, characterized in that: There are at least two flip arms (29). After the ends of the two flip arms (29) are flipped close to each other, the material taking head (12) extends into the sedimentation bottom tank (8) through the discharge opening (11), and the opening and closing member is in an open state.

7. The microorganism automated separation and cultivation equipment according to claim 1, characterized in that: Both ends of the sedimentation bottom tank (8) are provided with elastic clamping pieces (34), and the clamping ends of the elastic clamping pieces (34) match with the outer end surface of the storage tube (7).

8. The microorganism automated separation and cultivation equipment according to claim 3, characterized in that: The fixing seat (19) is also provided with a stabilizing portion for positioning the valve cover (9), the stabilizing portion comprising: A guide rod (35), wherein the guide rod (35) is mounted on the fixing seat (19); A mounting block (36), wherein the mounting block (36) is slidably disposed on the guide rod (35); a spring (37), wherein the spring (37) is sleeved on the guide rod (35), one end of the spring (37) abuts against the mounting block (36), and the other end of the spring (37) abuts against the fixing seat (19); A clamping plate (38) is mounted on the upper end of the mounting block (36), and the edge end of the valve cover (9) is clamped on the clamping plate (38).

9. The microorganism automated separation and cultivation equipment according to claim 1, characterized in that: A plurality of positioning blocks (39) are circumferentially mounted on the inner wall of the top cover (3), and a plurality of positioning blocks (39) are provided with positioning grooves (40), and a plurality of positioning grooves (40) are matched with the outer wall of the storage cylinder (7).

10. The microorganism automated separation and cultivation equipment according to claim 9, characterized in that: A side end of the valve cover (9) is provided with an avoidance groove (41), and the groove depth of the avoidance groove (41) is not less than the extended length of the positioning groove (40).

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