Device and method for collecting root exudates in initial stage of plant growth

Abstract

The invention relates to a device and method for collecting root exudates in the initial stage of plant growth, and belongs to the technical field of collection of root exudates, the device comprises a square vessel, a central incubator and a seed container which are in lap joint together, and a liquid injection pipe is further integrally formed on the side wall of the central incubator. In the operation process, different appropriate culture modes can be adopted for different growth stages of the plant from the seed germination stage, influences of various interference factors such as culture conditions and operation methods in the root exudate collection process are avoided, and meanwhile the function of observing the growth state of the plant root system is achieved. Plant root exudates at different parts in different stages can be collected, and comprehensiveness, accuracy and matching of collection of the root exudates in the initial growth stage of plants are guaranteed. And meanwhile, the device is designed into a simple detachable structure and has the characteristics of high operability and convenience in disassembly and maintenance.

Description

Technical Field

[0001] The present invention belongs to the technical field of collecting root secretions, and in particular relates to a device and method for collecting root secretions in the early growth stage of plants. Background Art

[0002] Root exudates are the medium for the exchange of substances, energy flow and information between plants and the surrounding environment, and are closely related to the growth regulation of plants. Collecting and studying the characteristics of root exudates components has important practical guiding significance for rationally and effectively regulating the plant rhizosphere system, maximizing the ecological function of plant roots, improving the efficiency of nutrient resource utilization and crop productivity, and achieving sustainable development of agricultural production.

[0003] At present, the collection technology of root secretions is mainly divided into soil culture, sand culture and nutrient solution culture (hydroponics), and each method has its own advantages and disadvantages. The soil culture method collects more comprehensive secretions, but the collection volume is small, the collection process is relatively complicated, and the operation precision is required to be high; the sand culture method is relatively simple to operate, but it is subject to greater resistance during the growth of plant roots, and cannot accurately reflect the secretion situation in the natural growth environment, and the sand washing and panning process is cumbersome and easy to introduce impurities; the hydroponics method is simple and easy to operate, and it is easy to eliminate the interference of foreign substances, but the roots of plants are in a state of flooding for a long time, which is prone to hypoxia and root rot, and it is quite different from the actual soil conditions. On the other hand, in the process of collecting root secretions, the accuracy of samples collected by the existing root secretion collection device is also disturbed by many factors, such as microbial contamination, impurities in the culture solution, etc., especially for plants in the early growth stage after seed germination, the root system is fragile and the growth rate is fast. During the operation, it is necessary to consider minimizing the disturbance to the plant root system, reducing the damage or pressure to the root system, and avoiding affecting the normal growth and secretion function of the plant. In order to reduce the interference factors in the plant cultivation process and improve the accuracy and operability of collecting root exudates, a simple collection device and method for root exudates in the early stage of plant growth are proposed. Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a device and method for collecting root secretions in the early stage of plant growth.

[0005] The technical solution adopted by the invention is: a device and method for collecting root secretions in the early stage of plant growth, the technical key points of which are: it includes a square dish with an open top, a sealing port is arranged at the bottom of the square dish, and a sealing plug is provided at the sealing port which is threadedly connected to it; a cover plate with a central culture container is overlapped on the square dish, the central culture container extends into the interior of the square dish, and injection tubes are integrally formed on the four side walls of the central culture container, and an injection propeller is inserted into the upper end of the injection tube, the elbow of the injection tube faces the inner cavity of the central culture container and is sealed by a rubber sealing plug; a seed holder is overlapped at the top opening of the central culture container, the seed holder has an upper edge which is slightly larger than the upper opening of the central culture container and is used to overlap the central culture container, the bottom of the seed holder is basin-shaped and sinks toward the interior of the central culture container, and the bottom of the seed holder is a grid structure In the above scheme, the lengths of the injection tubes located on the four outer walls of the central culture vessel are set according to the length of the root system, so that the elbows located at the bottom of the central culture vessel correspond to different collection positions of the root system.

[0006] In the above scheme, the cover plate, central culture vessel, injection tube and sealing plug are all made of quartz glass.

[0007] In the above solution, the seed container is made of PVC material.

[0008] A method for collecting root secretions in the early stage of plant growth, the technical main points of which are: comprising the following steps: Spread medical absorbent cotton or gauze on the grid at the bottom of the seed holder, and evenly sow the plant seeds on the cotton or gauze; add hydroponic nutrient solution that can soak the cotton or gauze and always soak the plant seeds in the central culture vessel to promote seed germination; after the plant seeds germinate and grow roots, maintain the culture medium renewal frequency according to the root system to maintain normal plant growth; take out the seed germination dish at the collection period, open the bottom sealing port of the central culture vessel, release the culture medium and clean the inside of the central culture vessel, rinse the roots to remove the nutrient matrix and ensure that the roots are not damaged; use different injection tubes corresponding to different collection parts to rinse and collect the root secretions, finally take out the seed germination dish and the culture dish, choose to directly pour the collection dish or open the bottom sealing plug of the collection dish to obtain the root secretion collection liquid for subsequent operations.

[0009] The beneficial effects of the present invention are as follows: the root secretion collection device and method for the initial stage of plant growth of the present invention comprises a square dish, a central culture container and a seed container which are overlapped together, wherein a liquid injection tube is integrally formed on the side wall of the central culture container, and different suitable culture methods can be adopted for different growth stages of plants starting from the seed germination period during operation, so as to avoid the influence of various interfering factors such as culture conditions and operation methods in the process of collecting root secretions, and at the same time, it has the function of observing the growth state of plant roots, and can collect plant root secretions from different parts at different stages, so as to ensure the comprehensiveness, accuracy and matching of the root secretion collection in the initial stage of plant growth. At the same time, the device is designed as a simple detachable structure, which has the characteristics of strong operability and convenient disassembly and maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0011] Figure 1 This is a schematic diagram of the structure of a device for collecting root secretions in the early stage of plant growth according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the structure of the central culture device in an embodiment of the present invention; Figure 3 This is a schematic diagram of the structure of a seed holder in an embodiment of the present invention; Explanation of the serial numbers in the figure: 1 square dish, 2 first sealing plug, 3 cover plate, 4 central culture vessel, 5 injection tube, 6 rubber sealing plug, 7 injection pusher, 8 second sealing plug, 9 seed holder. DETAILED DESCRIPTION

[0012] To make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the following is a brief description of the present invention in conjunction with the attached Figure 1-Figure 3 The present invention is further described in detail with specific implementation modes.

[0013] The root secretion collection device of the plant growth initial stage of this embodiment uses a square dish 1 as a collection dish. The square dish 1 is a square structure with an open top. A circular plugging port is set at the bottom center of the square dish 1. A first sealing plug 2 is installed at the circular plugging port. The plugging method is threaded rotation. The side wall, bottom and sealing plug 2 of the square dish 1 are made of quartz glass. The use of quartz glass material does not cause impurities to interfere with the collection liquid, and at the same time meets the need to observe the root growth status during plant cultivation and collection. The structure of the culture dish 1 being fully open at the top and having a plugging port at the bottom facilitates the replacement of culture substrates of different forms.

[0014] It also includes a cover plate 3 whose size is slightly larger than the upper edge of the collecting dish. The cover plate 3 is used as a culture dish and can be directly mounted on the square dish 1 and can be taken and placed at any time. A central culture container 4 with an open top is integrally formed on the cover plate 3. The four walls and the bottom of the central culture container 4 are connected to form a square structure and are located in the inner cavity of the square dish 1, which is convenient for direct taking and cleaning. An injection pipe 5 is respectively provided on the four side walls of the central culture container 4. The injection pipe 5 and the central culture container 4 are an integrally formed structure, and the injection pipe 5 is arranged in sequence at different heights, that is, corresponding to different parts of the plant root system, for pouring nutrient solution or distilled water. An elbow facing the inner cavity of the central culture container 4 is provided below the injection pipe 5. The elbow has a cylindrical liquid outlet head, and the diameter of the elbow to the liquid outlet head portion gradually decreases. A rubber sealing plug 6 is provided at the liquid outlet head. An injection propeller 7 made of PVC+rubber material is provided at the upper end of the injection pipe 5 to facilitate the control of the elution speed and flow rate to ensure that the flushing process does not cause damage to the root system. A circular plugging port is provided at the bottom center of the central culture vessel 4, and a second plugging port is installed at the circular plugging port to release the culture matrix, and the installation method is threaded rotation. The collection dish 1 adopts a square appearance and a plugging port at the bottom, considering the two different acquisition requirements of the collected liquid, namely, direct pouring or bottom overflow. The cover plate 3, the central culture vessel 4 and the injection tube 5 are made of quartz glass, so as to prevent impurities from interfering with the collected liquid, and at the same time meet the requirements of observing the root growth status during plant cultivation and collection.

[0015] This embodiment uses a seed holder 9 as a seed germination dish. The seed holder 9 is made of PVC material, with an open top, and the surroundings and bottom are 1 cm sunken in a basin shape. The upper edge size is slightly larger than the top opening edge of the central culture container 4. It can be directly placed on the central culture container 4 and can be taken and placed at any time. The bottom is a square grid design to ensure the germination space of plant seeds and the nutrient supply of the required nutrient solution to avoid inhibiting the growth of the initial plant root system. The padding material (medical absorbent cotton, gauze, etc.) and the density of germinating seeds can be selected according to needs.

[0016] The seed germination dish, culture dish and collection dish of this embodiment are connected to each other by mounting, not fixed connection. Considering the high frequency demand for culture matrix renewal and collection in the early stage of plant growth, the germination, culture and collection parts can be partially adjusted at any time to change plant species, update culture matrix, obtain collection liquid, etc. The independent design of each part ensures that it can be disassembled at any time, which improves the simplicity of operation, cleaning and maintenance of the device.

[0017] A method for collecting root secretions in the early stage of plant growth in this embodiment includes the following steps: Two layers of medical absorbent cotton or gauze are laid flat on the grid at the bottom of the seed holder 9, and the plant seeds are evenly sown on the cotton or gauze. After the injection nozzle of the central culture container 4 is blocked, the hydroponic nutrient solution is added to ensure that the nutrient solution soaks the cotton or gauze and always soaks the plant seeds. After the plant seeds germinate and grow roots, the cotton or gauze is removed, and the central culture container 4 is taken out. According to the growth status of the plant and the operation requirements, the culture medium is replaced with a nutrient solution (if there is a need to observe the growth status of the root system) or a mixture of culture soil or vermiculite and nutrient solution, and the replacement frequency is maintained to maintain the normal growth of the plant. When the collection period comes, take out the seed germination dish, open the bottom seal of the central culture vessel 4, release the culture medium and clean the inside of the central culture vessel 4 with distilled water, use distilled water to simply rinse the roots to remove the nutrient matrix and ensure that the roots are not damaged, take out the rubber seal 6 at the injection tube 5, then put the culture dish, seed germination dish and plants back on the collection dish, add distilled water to different injection tubes corresponding to different collection parts as needed, push the injection propeller 7 to rinse and collect the root secretions, finally take out the seed germination dish and culture dish, choose to directly dump the collection dish or open the bottom seal 2 of the collection dish to obtain the root secretion collection liquid for subsequent operations.

[0018] The present invention is used to collect root secretions from various parts of plants at various stages of early growth. The proposed device and collection method can avoid the influence of other impurities and other interfering factors during the cultivation and operation process to the greatest extent, meet the needs of observing the growth status of the roots during the plant cultivation and collection process, and meet the high-frequency needs of culture matrix replacement and root secretion collection in the early growth of plants. The components of each part of the device are independently detachable to ensure the simplicity of operation, cleaning and maintenance throughout the process. The present invention ensures the convenience and accuracy of collecting root secretions in the early growth of plants.

[0019] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A device for collecting root secretions in the early stage of plant growth, characterized in that: It comprises a square dish with an open top, a sealing opening is arranged at the bottom of the square dish, and a sealing plug is provided at the sealing opening which is threadedly connected thereto; a cover plate with a central culture container is overlapped on the square dish, and the central culture container extends into the interior of the square dish, and injection tubes are respectively integrally formed on the four side walls of the central culture container, and an injection propeller is inserted at the upper end of the injection tube, and the elbow of the injection tube faces the inner cavity of the central culture container and is sealed by a rubber sealing plug; a seed holder is overlapped at the top opening of the central culture container, and the seed holder has an upper edge which is slightly larger than the upper opening of the central culture container and is used to overlap the central culture container, and the bottom of the seed holder is basin-shaped and sinks toward the interior of the central culture container, and the bottom of the seed holder is a grid structure.

2. A device for collecting root secretions in the early stage of plant growth as claimed in claim 1, characterized in that: The lengths of the injection tubes located on the four outer walls of the central culture vessel are set according to the length of the root system, so that the elbows located at the bottom of the central culture vessel correspond to different collection positions of the root system.

3. A device for collecting root secretions in the early stage of plant growth as claimed in claim 1 or 2, characterized in that: The cover plate, central culture vessel, injection tube and sealing plug are all made of quartz glass.

4. A device for collecting root secretions in the early stage of plant growth as claimed in claim 3, characterized in that , the seed container is made of PVC material.

5. A method for collecting root secretions in the early stage of plant growth, implemented by the device as claimed in claim 1, characterized in that: The following steps are involved: Spread medical absorbent cotton or gauze on the grid at the bottom of the seed holder, and evenly sow the plant seeds on the cotton or gauze; add hydroponic nutrient solution that can soak the cotton or gauze and always soak the plant seeds in the central culture vessel to promote seed germination; after the plant seeds germinate and grow roots, maintain the culture medium renewal frequency according to the root system to maintain normal plant growth; take out the seed germination dish at the collection period, open the bottom sealing port of the central culture vessel, release the culture medium and clean the inside of the central culture vessel, rinse the roots to remove the nutrient matrix and ensure that the roots are not damaged; use different injection tubes corresponding to different collection parts to rinse and collect the root secretions, finally take out the seed germination dish and the culture dish, choose to directly pour the collection dish or open the bottom sealing plug of the collection dish to obtain the root secretion collection liquid for subsequent operations.

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