Rape sowing method capable of increasing content of protein and palmitic acid

Abstract

The invention relates to the technical field of rape sowing, and particularly discloses a rape sowing method for improving protein and palmitic acid content, which comprises the following steps: sowing in a dry land strip direct sowing mode in a test base, not thinning and singling after sowing, and adopting a strip application compound fertilizer as a base fertilizer; the seeding rate is 100 to 900 g / mu. Along with the increase of the seeding rate, the influence on the contents of protein, palmitic acid, stearic acid, erucic acid, oleic acid and linoleic acid of the Wanjiang 410 grains reaches a significant level, the contents of protein, palmitic acid and linoleic acid are gradually increased along with the increase of the seeding rate, and the erucic acid content and stearic acid content show a rule of first increasing and then decreasing along with the increase of the seeding rate; the influence on the eicosaenoic acid content of the Wanjiang 410 grains reaches a very significant level, the whole tends to increase firstly and then decrease along with the increase of the seeding rate, and the treated D6 is very significantly higher than that of other treatment groups. According to comprehensive consideration, it is considered that the seeding rate of the early-maturing rape Wanhuo410 is recommended to be 600 g / mu, and under the condition, the quality of the seeds of the Wanhuo410 is the best.

Description

Technical Field

[0001] The invention relates to the technical field of rapeseed sowing, and in particular to a rapeseed sowing method for increasing the protein and palmitic acid contents. Background Art

[0002] As an important oil crop in my country, the quality of rapeseed grains is directly related to the nutritional value and market competitiveness of edible oil. The quality indicators of rapeseed grains mainly include oil content, protein content, fatty acid composition and glucosinolate content, which are important bases for measuring the quality of rapeseed grains. There are many factors that affect the quality indicators of rapeseed grains. Song Yi et al. found that the amount of nitrogen fertilizer applied is one of the important factors affecting the quality indicators of rapeseed grains. With the increase of nitrogen fertilizer application, the protein content of rapeseed grains increased, the oil content and linoleic acid decreased, while glucosinolates, oleic acid, linolenic acid, erucic acid and saturated fatty acids (palmitic acid and stearic acid) showed an increasing trend, and the overall quality of rapeseed grains showed a decreasing trend. Yang Yihong found that in the case of excessive boron, the oil and protein content of rapeseed grains were significantly reduced. Jiang Fuyuan and other researchers found that herbicide ripening would reduce the thousand-grain weight and oil content of rapeseed grains, affecting the quality of rapeseed. Cai Xiaohui's research found that when the sowing time is delayed, the oil content, protein content and glucosinolate content of rapeseed grains decrease, but the fatty acid content is not significantly affected.

[0003] In recent years, a large number of scholars have focused their research on the factors affecting rapeseed grain quality indicators on aspects such as fertilization and sowing period. In the process of rapeseed cultivation, sowing amount, as a key agricultural management measure, also has a significant impact on the final yield and quality of rapeseed.

[0004] Therefore, the present invention uses the early-maturing rapeseed variety Wanyou 410 as the experimental material, and explores the influence of sowing amount on the grain quality indicators of the early-maturing rapeseed variety Wanyou 410 through different sowing amount treatments, in order to provide a reference for the cultivation of high-quality rapeseed in Wanzhou, Chongqing and similar ecological areas. Summary of the invention

[0005] In view of the technical problems mentioned in the background technology, the present invention proposes a rapeseed sowing method for increasing the protein and palmitic acid content.

[0006] The present invention adopts the following technical solution:

[0007] A method for sowing rapeseed for increasing the protein and palmitic acid content comprises the following steps:

[0008] The experimental base adopts dryland direct seeding method, and the sowing time is September 30 every year. After sowing, there is no thinning and transplanting, and the base fertilizer is strip-applied compound fertilizer;

[0009] The sowing rate is 100-900g / mu.

[0010] Preferably, the rapeseed variety sown is Wanyou 410.

[0011] Preferably, the base fertilizer is 20 kg / mu of strip-applied compound fertilizer, which is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0012] Preferably, the sowing rate is 200-800 g / mu.

[0013] Preferably, the sowing rate is 300-700 g / mu.

[0014] Preferably, the sowing rate is 400-700 g / mu.

[0015] Preferably, the sowing rate is 500-700 g / mu.

[0016] Preferably, the sowing rate is 600g / mu.

[0017] Preferably, the test base is at an altitude of 350 meters.

[0018] Preferably, the fertility of the soil in the test base before the test is: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and available potassium 99.3 mg·kg-1.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention explores the effect of seeding amount on the quality index of early-maturing rapeseed Wanyou 410 grains, which is of great significance to rapeseed cultivation. The present invention determines the changes in the quality indexes of Wanyou 410 grains under different seeding amount treatments: oil content, protein content, glucosinolate content and fatty acid composition. The results show that the increase in seeding amount has no significant effect on the oil content, glucosinolate and linolenic acid content of Wanyou 410 grains; the effect on the protein, palmitic acid, stearic acid, erucic acid, oleic acid and linoleic acid content of Wanyou 410 grains reaches a significant level, and the protein, palmitic acid and linoleic acid contents gradually increase with the increase in seeding amount, the erucic acid content and stearic acid content show a law of first increasing and then decreasing with the increase in seeding amount, and the oleic acid content has no obvious law with the increase in seeding amount; the effect on the eicosenoic acid content of Wanyou 410 grains reaches an extremely significant level, and with the increase in seeding amount, it shows an overall trend of first increasing and then decreasing, and treatment D6 is extremely significantly higher than other treatment groups. After comprehensive consideration, the present invention believes that the recommended sowing rate of early-maturing rapeseed Wanyou 410 is 600g / mu. Under this condition, the grain quality of Wanyou 410 is the best. DETAILED DESCRIPTION

[0021] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] The present invention provides a method for sowing rapeseed for increasing the protein and palmitic acid contents, comprising the following steps:

[0023] The experimental base adopts dryland direct seeding method, and the sowing time is September 30 every year. After sowing, there is no thinning and transplanting, and the base fertilizer is strip-applied compound fertilizer;

[0024] The sowing rate is 100-900g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0025] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which is a cabbage-type edible oil variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 μmol / g.

[0026] In this embodiment, the base fertilizer is 20 kg / mu of strip-applied compound fertilizer, which is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0027] In this embodiment, the altitude of the test base is 350 meters.

[0028] In this embodiment, the fertility of the soil in the test base before the test was: pH 7.1, moisture 3.81%, organic matter 12.8 mg kg-1, alkaline nitrogen 50.5 mg kg-1, available phosphorus 14.1 mg kg-1, and available potassium 99.3 mg kg-1.

[0029] Example 1.

[0030] The present invention provides a method for sowing rapeseed with increased protein and palmitic acid content, comprising the following steps:

[0031] From September 2020 to May 2022, sowing was carried out at an experimental base at an altitude of 350 meters. The soil fertility conditions of the experimental base before the test were: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and quick-acting potassium 99.3 mg·kg-1. It was used as sweet potato before.

[0032] At the experimental base, dryland direct seeding is adopted. The sowing time is September 30th every year. After sowing, no thinning or transplanting is done. The base fertilizer is 20 kg / mu of compound fertilizer applied in strips. It is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0033] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which belongs to the cabbage-type edible oil type variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 micromoles / gram. The sowing rate is treatment D1: 100g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0034] Example 2.

[0035] The present invention provides a method for sowing rapeseed with increased protein and palmitic acid content, comprising the following steps:

[0036] From September 2020 to May 2022, sowing was carried out at an experimental base at an altitude of 350 meters. The soil fertility conditions of the experimental base before the test were: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and quick-acting potassium 99.3 mg·kg-1. It was used as sweet potato before.

[0037] At the experimental base, dryland direct seeding is adopted. The sowing time is September 30th every year. After sowing, no thinning or transplanting is done. The base fertilizer is 20 kg / mu of compound fertilizer applied in strips. It is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0038] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which belongs to the cabbage-type edible oil type variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 micromoles / gram. The sowing rate is treatment D2: 200g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0039] Example 3.

[0040] The present invention provides a method for sowing rapeseed with increased protein and palmitic acid content, comprising the following steps:

[0041] From September 2020 to May 2022, sowing was carried out at an experimental base at an altitude of 350 meters. The soil fertility conditions of the experimental base before the test were: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and quick-acting potassium 99.3 mg·kg-1. It was used as sweet potato before.

[0042] At the experimental base, dryland direct seeding is adopted. The sowing time is September 30th every year. After sowing, no thinning or transplanting is done. The base fertilizer is 20 kg / mu of compound fertilizer applied in strips. It is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0043] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which belongs to the cabbage-type edible oil variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 micromoles / gram. The sowing rate is treatment D3: 300g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0044] Example 4.

[0045] The present invention provides a method for sowing rapeseed with increased protein and palmitic acid content, comprising the following steps:

[0046] From September 2020 to May 2022, sowing was carried out at an experimental base at an altitude of 350 meters. The soil fertility conditions of the experimental base before the test were: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and quick-acting potassium 99.3 mg·kg-1. It was used as sweet potato before.

[0047] At the experimental base, dryland direct seeding is adopted. The sowing time is September 30th every year. After sowing, no thinning or transplanting is done. The base fertilizer is 20 kg / mu of compound fertilizer applied in strips. It is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0048] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which belongs to the cabbage-type edible oil type variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 micromoles / gram. The sowing rate is treatment D4: 400g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0049] Example 5.

[0050] The present invention provides a method for sowing rapeseed with increased protein and palmitic acid content, comprising the following steps:

[0051] From September 2020 to May 2022, sowing was carried out at an experimental base at an altitude of 350 meters. The soil fertility conditions of the experimental base before the test were: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and quick-acting potassium 99.3 mg·kg-1. It was used as sweet potato before.

[0052] At the experimental base, dryland direct seeding is adopted. The sowing time is September 30th every year. After sowing, no thinning or transplanting is done. The base fertilizer is 20 kg / mu of compound fertilizer applied in strips. It is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0053] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which belongs to the cabbage-type edible oil type variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 micromoles / gram. The sowing rate is treatment D5: 500g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0054] Example 6.

[0055] The present invention provides a method for sowing rapeseed with increased protein and palmitic acid content, comprising the following steps:

[0056] From September 2020 to May 2022, sowing was carried out at an experimental base at an altitude of 350 meters. The soil fertility conditions of the experimental base before the test were: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and quick-acting potassium 99.3 mg·kg-1. It was used as sweet potato before.

[0057] At the experimental base, dryland direct seeding is adopted. The sowing time is September 30th every year. After sowing, no thinning or transplanting is done. The base fertilizer is 20 kg / mu of compound fertilizer applied in strips. It is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd. The N-P2O5-K2O ratio is 25-7-8-5 (%), and the trace elements nutrients mainly include B and Ca, Mg, Zn, and S.

[0058] In this embodiment, the rapeseed variety sown is the early-maturing rapeseed variety "Wanyou 410" bred by Chongqing Three Gorges Academy of Agricultural Sciences, which belongs to the cabbage-type edible oil type variety. The registered oil content of the variety is 45.56%, and the fatty acid composition includes oleic acid content of 58.20%, linoleic acid content of 19.80%, linolenic acid content of 13.60%, erucic acid content of 0.04%, and glucosinolate content of 13.63 micromoles / gram. The sowing rate is treatment D6: 600g / mu, and the area of ​​each treatment plot is 13.32m 2 .

[0059] Example 7.

[0060] The difference between Example 7 and Example 6 is that the sowing amount of Example 7 is treatment D7: 700 g / mu, and the other conditions are the same.

[0061] Example 8

[0062] The difference between Example 8 and Example 6 is that the sowing amount in Example 8 is treatment D8: 800 g / mu, and the other conditions are the same.

[0063] Example 9.

[0064] The difference between Example 9 and Example 6 is that the sowing amount of Example 9 is treatment D9: 900 g / mu, and the other conditions are the same.

[0065] Index determination:

[0066] All data are the average values ​​of the two-year experiment. After the rapeseed grains were harvested at maturity, the quality of the rapeseed grains harvested in each Example 1-9 was detected using the FOSS new generation NIRSTM DS2500F near-infrared multifunctional quality analyzer, mainly including oil content, protein content, glucosinolate content; fatty acid composition: palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, eicosenoic acid and erucic acid content.

[0067] Effect of seeding amount on oil content, protein content and glucosinolate content of Wanyou 410:

[0068] As shown in Table 1, the effect of seeding rate on the oil content and glucosinolate content of Wanyou 410 was not significant, and with the increase of seeding rate, the oil content and glucosinolate content of Wanyou 410 generally showed a trend of gradual decrease. The highest oil content was 45.9% in treatment D1 (100g / mu), and the lowest was 45.48% in treatment D9 (900g / mu), with a difference of 0.42%. The range of glucosinolate content was 16.76umol / g-19.45umol / g, which was within the standard range of double-low rapeseed (<30umol / g).

[0069] The sowing amount had a significant effect on the protein content of Wanyou 410. With the increase of sowing amount, the protein content of Wanyou 410 basically showed a gradually increasing trend. The protein content from low to high was D2: 21.17%, D1: 21.20%, D3: 21.36%, D7: 21.49%, D5: 21.50%, D4: 21.59%, D8: 21.66%, D6: 21.71%, and D9: 22.49%. The protein content of treatment D9 was significantly higher than that of treatment D2, with a difference of 1.32 percentage points.

[0070] Table 1 Effect of seeding rate on oil content, protein content and glucosinolate content of Wanyou 410

[0071]

[0072]

[0073] Note: Data represent (two-year mean ± standard deviation). Data in the same column marked with the same / different lowercase letters indicate no significant difference / significant difference (P<0.05). Data in the same column marked with the same / different uppercase letters indicate no significant difference / extremely significant difference (P<0.01).

[0074] Effect of seeding rate on the contents of palmitic acid, stearic acid, eicosenoic acid and erucic acid in Wanyou 410:

[0075] As shown in Table 2, the effect of seeding amount on the palmitic acid, stearic acid and erucic acid content of Wanyou 410 reached a significant level, and the effect on the eicosenoic acid content reached an extremely significant level. With the increase of seeding amount, the test results showed that the palmitic acid content of Wanyou 410 basically showed a gradually increasing trend, with the maximum value of treatment D9: 4.51%, and the minimum value of treatment D6: 4.37%, and D9 was significantly higher than D6, with a value of 0.14 percentage points; the stearic acid and erucic acid content of Wanyou 410 basically showed a law of first increasing and then decreasing, and the maximum value of stearic acid content was treatment D2: 1.23%, and the minimum value was treatment D6: 0.78%, with a difference of 0.45 percentage points between the maximum and minimum values. The order of erucic acid content from high to low is D2: 0.75%, D6: 0.64%, D3: 0.53%, D5: 0.47%, D4: 0.46%, D1: 0.45%, D8: 0.41%, D7: 0.37%, D9: 0.29%, all of which are within the standard range of double-low rapeseed (<3%), and D2 is significantly higher than D7 and D9, with differences of 0.38 percentage points and 0.46 percentage points, respectively; the overall eicosenoic acid content basically showed a trend of first increasing and then decreasing, with the maximum value being 5.71% in treatment D6 and the minimum value being 2.14% in treatment D8, and the eicosenoic acid content of D6 and D8 differing by 3.57 percentage points, and treatment D6 is also significantly higher than other treatments.

[0076] Table 2 Effect of seeding rate on the content of palmitic acid, stearic acid, eicosenoic acid and erucic acid in Wanyou 410:

[0077]

[0078]

[0079] Note: Data represent (two-year mean ± standard deviation). Data in the same column marked with the same / different lowercase letters indicate no significant difference / significant difference (P<0.05). Data in the same column marked with the same / different uppercase letters indicate no significant difference / extremely significant difference (P<0.01).

[0080] Effect of seeding rate on the content of oleic acid, linoleic acid and linolenic acid in Wanyou 410:

[0081] As shown in Table 3, the effect of seeding amount on the oleic acid and linoleic acid content of Wanyou 410 reached a significant level, but had no significant effect on the linolenic acid content. As the seeding amount increased, there was no obvious regularity in the effect on the oleic acid content of Wanyou 410. The oleic acid content of treatment D3 was the highest at 52.81%, and the oleic acid content of treatment D6 was the lowest at 50.18%, and the oleic acid content of treatment D3 was significantly higher than that of treatment D6 by 2.63 percentage points.

[0082] With the increase of sowing amount, the linoleic acid content showed a gradual increase as a whole. The linoleic acid content of treatment D9 was the highest at 23.78%, and the linoleic acid content of treatment D6 was the lowest at 22.51%. Treatment D9 was significantly higher than treatment D6, with a difference of 1.27 percentage points.

[0083] With the increase of sowing amount, the overall linolenic acid content basically showed a trend of first increasing and then decreasing. The linolenic acid content of treatment D6 was the highest at 12.44%, and the linolenic acid content of treatment D3 was the lowest at 12.03%, and treatment D6 was 0.41 percentage points higher than treatment D3.

[0084] Table 3 Effect of seeding rate on the content of oleic acid, linoleic acid and linolenic acid in Wanyou 410:

[0085]

[0086] Note: Data represent (two-year mean ± standard deviation). Data in the same column marked with the same / different lowercase letters indicate no significant difference / significant difference (P<0.05). Data in the same column marked with the same / different uppercase letters indicate no significant difference / extremely significant difference (P<0.01).

[0087] In the present invention, with the increase of sowing amount, the effect on the oil content and glucosinolate content of Wanyou 410 seeds is not significant, and the overall trend is gradually decreasing, while the effect of sowing amount on the palmitic acid content, linoleic acid content and protein content of Wanyou 410 seeds reaches a significant level, and the palmitic acid content, linoleic acid and protein content all gradually increase with the increase of sowing amount. The erucic acid content, stearic acid content and eicosenoic acid content of Wanyou 410 basically show a law of first increasing and then decreasing with the increase of sowing amount, and the sowing amount has a significant effect on the erucic acid content and stearic acid content, and has a very significant effect on eicosenoic acid. The effect of sowing amount on the oleic acid content of Wanyou 410 reaches a significant level, but there is no obvious pattern, and the highest content of oleic acid is 52.81% in treatment D3 (300g / mu).

[0088] Domestic rapeseed experts have proposed four indicators for the quality of high-quality rapeseed: (1) low erucic acid (less than 1%), low glucosinolates (less than 30umol per gram of rapeseed cake, excluding indole glucosinolates); (2) high oil content (more than 45%); (3) high protein (more than 28% of the seed weight, or more than 48% of the cake weight); (4) oleic acid content of more than 60%. The present invention believes that when early-maturing rapeseed Wanyou 410 is sown in Wanzhou, Chongqing and similar ecological areas, the recommended sowing rate is 600g / mu. Under this condition, the quality of Wanyou 410 grains is the best, with an erucic acid content of 0.64%, a glucosinolate content of 16.76umol / g, a linolenic acid content of 12.44%, an oil content of 45.81%, a protein content of 21.71%, and an oleic acid content of 50.18%. However, the sowing amount not only affects the quality indicators of rapeseed grains, but also affects the growth, development and yield of rapeseed. Therefore, in the actual production process, we need to consider comprehensively to achieve high yield and high quality of rapeseed.

[0089] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0090] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A method for sowing rapeseed to increase the content of protein and palmitic acid, characterized in that: The following steps are involved: The experimental base adopts dryland direct seeding method, and the sowing time is September 30 every year. After sowing, there is no thinning and transplanting, and the base fertilizer is strip-applied compound fertilizer; The sowing rate is 100-900g / mu.

2. A rapeseed sowing method for increasing protein and palmitic acid content according to claim 1, characterized in that: The rapeseed variety sown is Wanyou 410.

3. A rapeseed sowing method for increasing protein and palmitic acid content according to claim 1, characterized in that: The base fertilizer is 20 kg / mu of strip-applied compound fertilizer, which is the "Yishi Zhuang" rapeseed-specific slow-release formula fertilizer developed by Huazhong Agricultural University and produced by Hubei Yishi Zhuang Agricultural Technology Co., Ltd.

4. A rapeseed sowing method for increasing protein and palmitic acid content according to claim 1, characterized in that: The sowing amount is 200-800g / mu.

5. A rapeseed sowing method for increasing protein and palmitic acid content according to claim 4, characterized in that: The sowing amount is 300-700g / mu.

6. A rapeseed sowing method for increasing the protein and palmitic acid content according to claim 5, characterized in that: The sowing amount is 400-700g / mu.

7. A rapeseed sowing method for increasing the protein and palmitic acid content according to claim 6, characterized in that: The sowing amount is 500-700g / mu.

8. A rapeseed sowing method for increasing the protein and palmitic acid content according to claim 7, characterized in that: The sowing rate is 600g / mu.

9. A rapeseed sowing method for increasing the protein and palmitic acid content according to claim 1, characterized in that: The test base is 350 meters above sea level.

10. The method for sowing rapeseed for increasing the content of protein and palmitic acid according to claim 1, characterized in that: The fertility of the soil at the test base before the test was as follows: pH 7.1, moisture 3.81%, organic matter 12.8 mg·kg-1, alkaline nitrogen 50.5 mg·kg-1, available phosphorus 14.1 mg·kg-1, and available potassium 99.3 mg·kg-1.

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