A traditional Chinese medicine complex for preventing and controlling mycotoxin pollution in mutton sheep feed and its preparation method

Abstract

The present invention discloses a traditional Chinese medicine complex for preventing and treating mycotoxicosis in livestock, which is prepared by proportionally mixing and uniformly preparing cortex pseudolaricis, sophora flavescens, astragalus membranaceus, corydalis bungeana, polygonum cuspidatum, glucose oxidase and sodium diacetate as raw materials according to the different characteristics of each traditional Chinese medicine, and then vacuum drying to form a powder. The formula and process of the present invention are unique and scientific, and the treatment effect is remarkable.

Description

Technical Field

[0001] The present invention belongs to the field of feed additives, and particularly relates to a traditional Chinese medicine complex for preventing and treating mold toxin pollution in mutton sheep feed and a preparation method thereof. Background Art

[0002] In animal feed and food, mycotoxins are an important class of harmful substances. They are metabolites produced by certain molds (such as Aspergillus, Penicillium, and Fusarium), and can be produced in agricultural products such as grains, feeds, and nuts. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), globally, nearly 25% of forage crops are contaminated by mycotoxins to varying degrees every year, and 2% of forage crops will directly lose their economic value due to severe contamination, with losses reaching up to tens of billions of US dollars. In China, the detection rate of mycotoxins in silage feed is as high as 70% - 100%, and forage raw materials and hay are all contaminated by mycotoxins to varying degrees, with as few as 2 - 3 kinds detected and as many as 7 - 8 kinds detected, especially in the hot and humid southern regions where the detection rate is higher than that in the north. In recent years, the pollution of mycotoxins in forage and compound feed has become increasingly serious, with the detection rate remaining high and the exceeding standard rate also showing an upward trend.

[0003] Mycotoxins can inhibit the immune system of animals, leading to a decline in their resistance to diseases. Mycotoxins may also cause reproductive problems, including infertility and miscarriage. Aflatoxin in mycotoxins is particularly known for its toxicity to the liver, which can cause liver enlargement and liver dysfunction; while ochratoxin mainly affects the kidneys, leading to renal failure. In addition, the presence of mycotoxins will cause a decrease in the feed intake of animals, thus directly affecting their growth rate and production performance. Due to the inhibition of feed digestion and absorption, the growth rate of animals will be significantly reduced, affecting their final body weight and meat quality. Mycotoxins not only affect the health and production performance of animals, but also cause significant economic losses. Due to the influence of mycotoxins, animals may require more veterinary care, increasing the economic burden on breeders. In addition, after mycotoxins enter the animal body with the feed, they will remain and accumulate in the body, leading to livestock poisoning, illness, and even death. At the same time, mycotoxins will also enter the human body through meat, eggs, milk, etc., causing serious health hazards such as cancer, deformities, infertility, etc.

[0004] In modern agricultural production, if feed is not kept dry and ventilated during storage, or the storage environment has a too high humidity, it is easy for the feed to get moldy. Feed contaminated with mycotoxins is usually discarded or treated harmlessly, which further increases the breeding cost. However, in some cases, livestock may still accidentally eat moldy or mycotoxin-contaminated feed. On the one hand, feed is usually stored in stacks. Although the moldy feed is removed, the non-moldy part may still be contaminated with mycotoxins. On the other hand, when feed is in short supply, farmers may be forced to use moldy feed to ensure that animals have enough food. Moreover, some farmers may lack awareness of the harm of moldy feed and think that as long as the animals are willing to eat, it can still be used.

[0005] Therefore, it is urgent to solve the problem of mycotoxin contamination in feed, reduce production costs and protect the health of livestock. Summary of the Invention

[0006] In order to solve the problems existing in the prior art, the present invention provides a traditional Chinese medicine complex for preventing and treating mycotoxin poisoning in livestock, with a unique and scientific formula and process, and remarkable effect in preventing livestock from being affected by mycotoxins.

[0007] For this, the present invention adopts the following technical solutions:

[0008] First, the present invention relates to a traditional Chinese medicine complex for preventing and treating mycotoxin contamination in livestock feed, including Pseudolarix bark, Sophora flavescens root, Astragalus membranaceus, Corydalis bungeana, Polygonum cuspidatum and an immune regulator.

[0009] Among them, the respective traditional Chinese medicine raw materials are:

[0010] Pseudolarix bark: It is the dried root bark or near-root bark of Pseudolarix kaempferi Gord. of the Pinaceae family. Pungent, warm. It belongs to the lung and spleen meridians. Expelling wind and removing dampness; killing insects and relieving itching.

[0011] Sophora flavescens root: It is the dried root of Sophora flavescens Ait. of the Leguminosae family. Bitter, cold. It belongs to the heart, liver, stomach, large intestine and bladder meridians. Clearing heat and drying dampness, killing insects, promoting diuresis.

[0012] Astragalus membranaceus: It is the dried root of Astragalus membranaceus (Fisch) Bge. var. mongholicus (Bge) Hsiao or Astragalus membranaceus (Fisch) Bge. of the Leguminosae family. Sweet, slightly warm. It belongs to the lung and spleen meridians. Tonifying qi and lifting yang, securing the exterior and stopping sweating, promoting diuresis to alleviate edema, promoting the production of body fluid and nourishing blood, promoting qi circulation to relieve pain, expelling toxins and discharging pus, astringing sores and promoting granulation.

[0013] Corydalis: The whole herb or root of Corydalis edulis Maxim, a plant of the Papaveraceae family. Bitter, astringent, and cool. It acts on the lung, kidney, and spleen meridians. It clears heat and detoxifies; kills insects and relieves itching.

[0014] Reynoutria japonica: The dried rhizome and root of Reynoutria japonica Houtt., a plant of the Polygonaceae family. Slightly bitter and slightly cold. It acts on the liver, gallbladder, and lung meridians. It promotes diuresis and reduces jaundice, clears heat and detoxifies, dispels stasis and relieves pain, and relieves cough and reduces phlegm.

[0015] Furthermore, the traditional Chinese medicine complex for preventing and controlling mycotoxin contamination in livestock feed includes 20 - 45 parts of Pseudolarix kaempferi, 20 - 45 parts of Sophora flavescens, 60 - 90 parts of Astragalus membranaceus, 10 - 35 parts of Corydalis, 20 - 45 parts of Reynoutria japonica, and 0.5 - 15 parts of immunomodulator.

[0016] Among them, the immune additive is selected from one or more of inulin, ginseng, vitamin E, glucose oxidase, sodium butyrate, sodium diacetate, EPA, DHA, and lactoferrin.

[0017] Furthermore, the traditional Chinese medicine complex for preventing and controlling mycotoxin contamination in livestock feed includes 30 parts of Pseudolarix kaempferi, 30 parts of Sophora flavescens, 70 parts of Astragalus membranaceus, 20 parts of Corydalis, 30 parts of Reynoutria japonica, and 6 parts of immunomodulator.

[0018] Among them, the immune additive is selected from glucose oxidase and sodium diacetate; the mass ratio of glucose oxidase to sodium diacetate is 1:5.

[0019] In the second aspect, the present invention relates to a traditional Chinese medicine complex for preventing and controlling mycotoxin contamination in livestock feed, which is prepared from 30 parts of Pseudolarix kaempferi, 30 parts of Sophora flavescens, 70 parts of Astragalus membranaceus, 20 parts of Corydalis, 30 parts of Reynoutria japonica, 1 part of glucose oxidase, and 5 parts of sodium diacetate.

[0020] In the third aspect, the present invention relates to a preparation method of a traditional Chinese medicine complex for preventing and controlling mycotoxin contamination in livestock feed, which is characterized by including the following steps:

[0021] ① Weigh Pseudolarix kaempferi, Sophora flavescens, Astragalus membranaceus, Corydalis, and Reynoutria japonica according to the formula ratio described in any of the foregoing aspects, vacuum dry, pulverize, and sieve to obtain a traditional Chinese medicine raw material powder for standby;

[0022] ② Weigh the immunomodulator according to the formula ratio, sieve to obtain an immunomodulator powder for standby;

[0023] ③ Mix the traditional Chinese medicine raw material powder and the immunomodulator powder, stir evenly and sieve to obtain the traditional Chinese medicine complex.

[0024] Fourthly, the present invention relates to the use of any one of the traditional Chinese medicine complexes for preventing and treating mycotoxin contamination in livestock feed described in the foregoing first aspect or second aspect, or the traditional Chinese medicine complex prepared by the preparation method described in the third aspect of the present invention in the preparation of a feed additive for preventing and treating mycotoxin contamination in livestock feed.

[0025] Furthermore, the livestock are mammals;

[0026] Preferably, the livestock are ruminants;

[0027] Preferably, the livestock are cattle, sheep, and horses.

[0028] Even further, the feed additive can inhibit the reduction in feed intake, decline in immunity, and occurrence of inflammatory reactions in livestock caused by mycotoxin contamination.

[0029] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0030] By reasonably formulating the traditional Chinese medicines Pseudolarix kaempferi, Sophora flavescens, Astragalus membranaceus, Corydalis edulis, and Polygonum cuspidatum with the immunomodulators glucose oxidase and sodium diacetate as raw materials, and through the reasonable combination of the different characteristics of the raw materials, the present invention can effectively prevent the reduction in feed intake, decline in immunity, and inflammatory reactions in livestock caused by accidentally eating moldy feed, can increase the feed intake of livestock, promote the improvement of livestock production performance, and prevent livestock from getting sick. Description of the Drawings

[0031] Figure 1 . Influence of different drug formulations on the toxicity of ZEA

[0032] Figure 2 . Mycotoxin content in each group of formulations

[0033] Figure 3 . Influence of each group of formulations on the growth performance of livestock

[0034] Figure 4 . Influence of each group of formulations on the immunoglobulins of livestock

[0035] Figure 5 . Influence of each group of formulations on the cytokines of livestock Detailed Embodiments

[0036] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. The raw materials, reagents, or devices used in the following embodiments can be obtained from conventional commercial channels or can be obtained by existing known methods without special instructions. The experimental methods without specific conditions are usually carried out under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise stated, the parts and percentages are by weight.

[0037] Optimization and Preparation of Traditional Chinese Medicine Complex Formula in Example 1

[0038] 1. Experimental samples

[0039] Dry each traditional Chinese medicine raw material in an oven. Take 10 g of each single traditional Chinese medicine. Soak each group in 8 times the volume of water of the weighed traditional Chinese medicine for 2 h. After boiling vigorously, continue to boil over low heat for 30 min, and filter with gauze. Keep the filtrate for later use. Then add 5 times the volume of sterilized water to the filter residue. After boiling, boil over low heat for 20 min. Combine the two filtrates and vacuum dry to obtain a solid for later use.

[0040] Zearalenone (ZEA) is dissolved in DMSO to prepare a ZEA solution with a concentration of 0.1 M. After aliquoting, store it in the dark at -20 °C for later use. Dilute it to the required concentration with PBS before use.

[0041] Table 1 Experimental drug formula

[0042] Raw material mg / mL Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Experimental Group 5 Experimental Group 6 Pseudolarix bark 30 30 90 30 30 30 Sophora flavescens 30 30 90 30 30 30 Astragalus membranaceus 70 70 / / 70 70 Corydalis bungeana 20 20 / 20 20 / Polygonum cuspidatum 30 30 / 30 / 30 Honeysuckle flower / / / / / 20 Artemisia capillaris / / / / 30 / Codonopsis pilosula / / / 70 / / Glucose oxidase 1 / 3 1 1 1 Sodium diacetate 5 / 3 5 5 5 Lactoferrin / 1 / / / / Sodium butyrate / 5 / / / /

[0043] According to the formula in Table 1, weigh the required mass of each raw material and dissolve it with PBS. The final concentration of each raw material in the solution is as shown in Table 1, and prepare it into a stock solution. Dilute each prepared stock solution of the experimental groups to 1 / 100 of the stock solution with PBS before adding it to the cell plate, and filter it with a 0.22 μm filter to remove bacteria and pyrogens.

[0044] 2. Experimental methods

[0045] MTT method:

[0046] Sheep lung fibroblasts (primary immortalized) cells OAR-L1 are cultured in DMEM medium (containing 10% FBS, 1% PS) at a constant temperature of 37 °C with 5% CO2;

[0047] Collect cells in the logarithmic growth phase, adjust the cell suspension concentration, and inoculate the cell suspension into a 96-well plate. Plate the cells to adjust the density of the cells to about 1×10 4 / Wells, with 100 μL of cell suspension in each well, incubated at 37 °C with 5% CO2 for 24 h. It was observed under the microscope that the cells adhered and grew. 10 μL of ZEA was added to the culture plate, with the final concentration of ZEA being 100 μM. After incubation for 1 h, 10 μL of the test solutions of experimental groups 1 to 6 were added to each well (so that the final concentration of the test solution added to the cells was μg / mL), and finally PBS was added as a blank control. The culture plate was placed in an incubator at 37 °C with 5% CO2 for culture. After 24 h, the culture plate was taken out, 10 μl of MTT (5 mg / mL) solution was added to each well, and the culture was continued for 4 h. The culture was terminated, and the culture medium in the wells was carefully aspirated. Then 100 μl of DMSO was added to each well, and it was placed on a shaker and shaken at a low speed for 10 min to fully dissolve the purple crystals. The absorbance values of each well were measured at 490 nm using a fully automatic microplate reader.

[0048] 3. Experimental results

[0049] The experimental results are as Figure 1 shown.

[0050] The results showed that ZEA could induce the death of OAR-L1, and the drug in experimental group 1 could significantly protect OAR-L1 cells from cell death caused by ZEA. The immune regulation in experimental group 1 was replaced in experimental group 2, and the cell protection effect disappeared. In experimental group 3, only pseudolaric acid bark, sophora flavescens, glucose oxidase, and sodium diacetate were retained, and the raw material dosage ratio was changed, and the cell protection effect disappeared. In experimental group 4, codonopsis pilosula, which is also a qi-tonifying medicine, was used to replace astragalus membranaceus. In experimental group 5, artemisia capillaris, which is also a dampness-draining medicine, was used to replace polygonum cuspidatum. In experimental group 6, honeysuckle flower, which is also a heat-clearing and detoxifying medicine, was used to replace corydalis. However, the results showed that replacing with medicinal flavors with similar or close efficacy made the cell protection effect of experimental group 1 disappear. Thus, it can be seen that the selected medicinal flavors and dosage ratios in the present invention have unexpected technical effects.

[0051] Verification of the Effect of Traditional Chinese Medicine Complex on Preventing and Treating Mycotoxin Poisoning in Livestock in Example 2

[0052] The following method was used to prepare the traditional Chinese medicine complex for verification:

[0053] ① Weigh 30 parts of pseudolaric acid bark, 30 parts of sophora flavescens, 70 parts of astragalus membranaceus, 20 parts of corydalis, and 30 parts of polygonum cuspidatum, vacuum dry, crush, and sieve to obtain the traditional Chinese medicine raw material powder for standby;

[0054] ② Weigh 1 part of glucose oxidase and 5 parts of sodium diacetate, sieve to obtain the immunomodulator powder for standby;

[0055] ③ Mix the traditional Chinese medicine raw material powder and the immunomodulator powder, stir evenly and sieve to obtain the traditional Chinese medicine complex.

[0056] 1. Experimental scheme

[0057] Prepare the feeds for each group according to the formula in Table 2.

[0058] Table 2 Feed Test Formula

[0059] Raw material / parts by mass Blank Control Group Model Control Group Traditional Chinese Medicine Complex Group Corn 555 290 282 Pit silage + Moldy corn / 265 260 Wheat middlings 20 20 20 Corn germ oil 200 200 196 Soybean meal 46% 145 145 142 Slow-release urea (200%) 5 5 5 Molasses 15 15 15 Limestone powder 10 10 10 Calcium hydrogen phosphate 5 5 5 Sodium chloride 10 10 10 Soybean oil 10 10 10 Premix (Z600) 25 25 25 Traditional Chinese Medicine Complex / / 20 Total 1000 1000 1000

[0060] 2. Experimental Grouping

[0061] The experiment was divided into a blank control group, a model control group, and a traditional Chinese medicine complex group.

[0062] Blank control group: Feed the formula of the blank control group in Table 1.

[0063] Model control group: Feed the formula of the model control group in Table 1, and replace part of the normal corn with moldy corn in this group's formula.

[0064] Traditional Chinese medicine complex group: Feed the formula of the traditional Chinese medicine complex group in Table 1, replace part of the normal corn with moldy corn in this group's formula, and add the prepared traditional Chinese medicine complex.

[0065] 3. Experimental Design

[0066] For the animal experiment, 8-month-old female Hu sheep from Inner Mongolia Shengle Animal Husbandry with an average weight of 32.80 kg were selected. The experimental animals were randomly divided into a blank control group (n = 10), a model control group (n = 10), and a traditional Chinese medicine complex group (n = 10), and were fed the formula of the blank control group in Table 1, the formula of the model control group in Table 1, and the formula of the traditional Chinese medicine complex group in Table 1, respectively. The experimental period was 90 days. Before the feeding experiment, the animals had a 10-day adaptation period to obtain an appropriate feed intake. During the experimental period, the animals could freely eat and drink.

[0067] 4. Weighing and Sample Collection

[0068] Before the start of the experiment, all the sheep were weighed by an automatic weighing system to obtain the initial weight. Then, the weight on the 90th day was weighed to calculate the daily weight gain. The remaining feed intake was collected every day to calculate the feed intake of the meat sheep. Jugular venous blood was collected on an empty stomach and placed in a 5 mL vacuum negative pressure tube, centrifuged at 3000 rpm for 8 min, the supernatant was taken, immediately stored in a -80 °C refrigerator, and sent to the laboratory for further analysis. At the end of the experimental period, 10 Hu sheep in each group were slaughtered for tissue sample collection. Within 20 minutes after slaughter, the left longissimus dorsi muscle and subcutaneous adipose tissue samples (about 0.5×0.5×0.5 cm 3 ) of the carcass were placed in a 2 mL freezing tube and immediately frozen in liquid nitrogen for subsequent analysis.

[0069] 5. Detection Indexes

[0070] 5.1 Mycotoxin Content in Each Group's Formula

[0071] Quantitative analysis was carried out by establishing a UPLC-QQQ-MS method, and the results were as follows Figure 2 shown. In the model control group feed with mildewed corn replacing normal corn, representative mycotoxins were significantly exceeded the standard. Among them, aflatoxin B1 (AFB1) was 4.5 ppb, about 5 times that of the blank group, the content of deoxynivalenol (DON) was 2 times that of the blank group; zearalenone (ZEA) was 7-8 times that of the blank group. The above results suggest that the modeling of this experiment was successful. Based on the formula of the model control group, a traditional Chinese medicine complex was added for intervention treatment

[0072] 5.2 Growth performance

[0073] Before the start and at the end of the formal experiment, the experimental mutton sheep were fasted on an empty stomach for 12 h, then weighed and recorded as the initial weight and final weight, which were used to calculate the average daily gain. During the experiment, the feeding amount and remaining feed amount of each replicate were accurately recorded every day to calculate the daily feed intake. The feed-to-gain ratio was calculated from the average daily feed intake and the average daily weight gain

[0074] Average daily gain = (average weight at the end of the experiment - average weight at the beginning of the experiment) / number of days of the experiment × number of heads in each group

[0075] Average daily feed intake = total feed consumption of each group / (number of days of the experiment × number of heads in each group)

[0076] Feed-to-gain ratio = average daily feed intake / average daily gain

[0077] 5.3 Blood immune indexes

[0078] On the morning before feeding on the day when the formal experiment ended, the sheep were fixed and 10 mL of blood was collected from the jugular vein of the mutton sheep with a vacuum blood collection tube. After centrifuging at 3000 r / min for 15 min, the upper serum was aspirated, aliquoted into 2 mL centrifuge tubes and labeled, and stored in a -20 °C refrigerator. According to the immune index kits of Nanjing Jiancheng Reagent Co., Ltd. and the automatic biochemical analyzer, the contents of serum immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor (TNF-α) were detected

[0079] 6. Experimental results

[0080] 6.1 Growth performance

[0081] As Figure 3 shown in A., the initial weights of Hu sheep in each group were close, with an average initial weight of 32.1 ± 0.6 kg. After 100 days of feeding treatment, the average weight of Hu sheep in the model control group decreased by 4.59 kg, with a decrease rate of 10.9%; compared with the model control group, the weight of the traditional Chinese medicine complex group increased by 4.76 kg, with an increase rate of 12.8% (the results are shown in Figure 3B). Compared with the blank control group, the average daily feed intake of the model control group decreased by 49.26 g, a decrease of 6.35%. Compared with the model control group, the average daily feed intake of the traditional Chinese medicine complex group increased by 19.6 g, an increase of 2.69% (the results are shown in Figure 3 C). The average daily weight gain of the traditional Chinese medicine complex was significantly increased (the results are shown in Figure 3 D.).

[0082] From the above experimental results, it can be seen that compared with the model control group, the traditional Chinese medicine complex involved in the present invention significantly increased the final body weight and average daily weight gain of Hu sheep, suggesting that the traditional Chinese medicine complex in the present invention can effectively improve the growth performance of Hu sheep, and further reduce the feeding cost.

[0083] 6.2 Blood immune indexes

[0084] IgM, IgA and IgG are important indicators expressing the immunity of animal bodies. The decline of the immune ability of Hu sheep will enable pathogens to invade and thus affect the health of Hu sheep. IgM has important functions such as binding to antigens and hemolysis. IgA can play a role in resisting virus invasion and killing foreign viruses. IgG can regulate immune phagocytes and kill viruses.

[0085] As Figure 4 shown, the IgM, IgA and IgG of the meat sheep in the model control group showed extremely significant increases, indicating that feeding mold toxin-contaminated feed would lead to a significant decline in the immune ability of Hu sheep. Compared with the model control group, the serum IgM, IgA and IgG levels of Hu sheep fed with the traditional Chinese medicine complex group showed significant decreases, indicating that the immune ability of Hu sheep fed with the traditional Chinese medicine complex could be significantly higher than that of the model group of Hu sheep, enhancing the immune ability of Hu sheep.

[0086] Low levels of IL-10 often coexist with high levels of pro-inflammatory cytokines TNF-α and IL-6, indicating an imbalanced immune state.

[0087] As Figure 5 shown, the TNF-α and IL-6 of the Hu sheep in the model control group showed extremely significant increases, and the IL-10 showed extremely significant decreases, indicating that feeding mold toxin-contaminated feed would lead to active inflammatory reactions and immune imbalance in Hu sheep. Compared with the model control group, the serum TNF-α and IL-6 of Hu sheep fed with the traditional Chinese medicine complex group showed extremely significant decreases, while the IL-10 showed extremely significant increases, indicating that feeding the traditional Chinese medicine complex could effectively improve the inflammatory reactions and immune imbalance caused by mold toxin contamination, reduce inflammatory reactions and enhance immunity.

[0088] In summary, the traditional Chinese medicine complex described in the present invention can effectively protect mutton sheep cells from cell death caused by ZEA and has a significant cell protection effect. When the traditional Chinese medicine complex is used as a feed additive and added to moldy feed, it can significantly reduce the negative impacts of moldy feed on the production performance of mutton sheep, such as feed intake and weight gain. At the same time, it can also significantly enhance the immunity of livestock and significantly inhibit the inflammatory response caused by livestock accidentally eating moldy feed. Thus, it can be seen that the traditional Chinese medicine complex provided by the present invention can effectively avoid various adverse reactions caused by livestock accidentally eating moldy feed during the production process, greatly reducing the breeding cost of farmers, and having broad prospects for economic and social benefits.

[0089] Special note: The attached drawings of the specification Figure 2 、 Figure 3 、 Figure 4 and Figure 5 The Chinese herbal medicine additive groups in

[0090] The above content is a further detailed description of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention pertains, without departing from the concept of the present invention, several simple deductions or substitutions can still be made, and all should be regarded as belonging to the protection scope of the present invention.

Claims

1. A traditional Chinese medicine complex for preventing and controlling mycotoxin pollution in ruminant feed, which is prepared from 30 parts of Pseudolarix kaempferi, 30 parts of Sophora flavescens, 70 parts of Astragalus membranaceus, 20 parts of Corydalis edulis, 30 parts of Polygonum cuspidatum and 6 parts of immune regulator; The immune regulator is prepared from 1 part of glucose oxidase and 5 parts of sodium diacetate.

2. The preparation method of the traditional Chinese medicine complex according to claim 1, characterized in that, It includes the following steps: ① Weigh Pseudolarix kaempferi, Sophora flavescens, Astragalus membranaceus, Corydalis edulis and Polygonum cuspidatum according to the formula ratio, vacuum dry, crush and sieve to obtain a traditional Chinese medicine raw material powder for standby; ② Weigh the immune regulator according to the formula ratio, sieve to obtain an immune regulator powder for standby; ③ Mix the traditional Chinese medicine raw material powder and the immune regulator powder, stir evenly and sieve to obtain the traditional Chinese medicine complex.

3. Use of the traditional Chinese medicine complex described in claim 1 or the traditional Chinese medicine complex prepared by the preparation method described in claim 2 in the preparation of a feed additive for preventing and controlling mycotoxin pollution in ruminant feed.

4. The use according to claim 3, wherein the ruminant is cattle or sheep.

5. The use according to claim 3 or 4, wherein the feed additive can inhibit the reduction of feed intake, the decline of immunity and the occurrence of inflammatory reactions in ruminants caused by mycotoxin pollution.

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