Application of combined extract of rabdosia japonica and purslane in preparation of skin care product with whitening effect

Abstract

The invention discloses an application of a combined extract of rabdosia japonica and purslane in preparation of a skin care product with a whitening effect. Detection of the tyrosinase activity inhibition effect, the free radical scavenging rate and the moisturizing effect of the combined extract of rabdosia japonica and purslane shows the application of the combined extract in the skin care product with the whitening effect. The combined extract of rabdosia japonica and purslane has an inhibiting effect on tyrosinase activity and has a scavenging effect on free radicals, so that a whitening effect is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of new uses of a combined extract of blue-calyx chrysanthemum and purslane, and particularly relates to an application of the combined extract of blue-calyx chrysanthemum and purslane in preparing a skin care product with whitening effect. Background Art

[0002] Rabdosia japonica Haravar is a plant of the genus Rabdosia in the family Lamiaceae. The whole plant above ground is used as medicine. It is harvested in summer and autumn, impurities are removed, and it is dried in the sun. Its functions and indications are: clearing away heat and detoxifying, promoting blood circulation and removing blood stasis. It is used for colds, sore throats, tonsillitis, gastritis, hepatitis, mastitis, the initial onset of cancer (esophageal cancer, cardia cancer, liver cancer, breast cancer), amenorrhea, traumatic injuries, joint pain, and snake and insect bites. However, recent studies have found that among the monomer compounds extracted from Rabdosia japonica Haravar, masliic acid and quercetin have good antioxidant activity, and oleanolic acid and methyl salicylate also have certain antioxidant activity, so they can be considered to be added to skin care products to play a whitening effect. Portulaca oleracea L, also known as horse tongue vegetable, longevity vegetable, etc., is an annual fleshy herb of the genus Portulaca in the family Portulacaceae, and its dried above-ground parts are mostly used as medicine. As a traditional Chinese medicine, purslane has been used in folk medicine to treat various diseases since ancient times and has high medicinal value. Studies have shown that its active ingredients are mainly flavonoids, polysaccharides, alkaloids, organic acids, etc. As a Chinese herbal medicine, purslane has the characteristics of good safety and few side effects. Therefore, it has been widely used in the field of cosmetics. Analysis of purslane extracts detected phenolic acids and flavonoids such as caffeic acid, p-coumaric acid, ferulic acid, apigenin, and kaempferol. Bioactivity tests confirmed that fresh purslane extracts showed significant free radical scavenging ability. The spatial structure-efficiency of purslane polysaccharides was modified by acetylation to make it easier to provide hydroxyl groups and capture superoxide anions, thereby improving the antioxidant activity.

[0003] Patent document CN202010967691.8 discloses a blue-calyx spatholobi root extract, an extraction and separation method and its application, comprising the following components: 3-oxo-12-ene-28-ursolic acid 5.61-5.93 μg / g, ursolic acid 4.75-5.37 μg / g, oleanolic acid 4.82-5 μg / g, blue-calyx acetone 36.29-37.15 μg / g, oridonin 3 .2~3.52μg / g, linalool 2.72~2.86μg / g, blue calyx 11.32~11.76μg / g, quercetin 6.79~7.51μg / g, luteolin 6.23~6.63μg / g, apigenin 4.49~5.03μg / g; the blue calyx spatholobi root extract of the present invention can be used to prepare functional health products for lowering blood sugar, whitening, improving intelligence and strengthening the brain, and has good application prospects. However, the document does not have any relevant records about the blue calyx spatholobi root extract being used to prepare skin care products with whitening effects.

[0004] The patent document CN201911286752.8 discloses a whitening and freckle-removing composition, comprising the following components in mass fractions: 8-15 parts of Schisandra chinensis extract; 7-12 parts of Portulaca oleracea extract; 6-13 parts of Rheum palmatum root extract; 8-12 parts of Houttuynia cordata extract. The preparation method of the whitening and freckle-removing composition is as follows: the components of the whitening and freckle-removing composition are stirred and mixed evenly according to the mass fraction ratio to obtain the whitening and freckle-removing composition. The application of the whitening and freckle-removing composition is as follows: the whitening and freckle-removing composition is directly added to skin care products. By using Schisandra chinensis extract, Portulaca oleracea extract, Rheum palmatum root extract and Houttuynia cordata extract to cooperate with each other, it is beneficial to enhance the whitening and freckle-removing effect of the whitening and freckle-removing composition. Although the document discloses relevant records of using purslane extract to prepare skin care products with whitening effect, there is no relevant record about using the purslane extract and the blue-calyx oleracea root extract to form a composite extract for preparing skin care products with whitening effect. Moreover, the skin care combined effect achieved by the purslane extract and the blue-calyx oleracea root extract is not a conventional effect, and the function is difficult to predict. Summary of the invention

[0005] The invention aims to provide application of a combined extract of Isodon calyx and Portulaca oleracea in preparing a skin care product with whitening effect.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: application of a combined extract of Ipomoea aurea and Portulaca oleracea in the preparation of a skin care product with whitening effect.

[0007] It is further specified that the combined extract of Ipomoea aquatica and Portulaca oleracea has an inhibitory effect on tyrosinase activity and a scavenging effect on free radicals, thereby achieving a whitening effect.

[0008] It is further defined that the combined extracts of Isodon calyx and Portulaca oleracea are combined with the highly effective moisturizing ingredients of glycerin and trehalose to form a moisturizing system for hair care products, which can quickly penetrate into skin cells, promote cell hydration, and give the skin full elasticity and luster.

[0009] The present invention has the following advantages and beneficial effects: through whitening effect test, the combined extract of blue calyx fragrant tea and purslane has an inhibitory effect on tyrosinase activity, free radical scavenging rate and moisturizing effect to illustrate its application in skin care products with whitening effect; the combined extract of blue calyx fragrant tea and purslane has an inhibitory effect on tyrosinase activity and a scavenging effect on free radicals, thereby achieving a whitening effect. In the field of skin care product science, the combined extract of blue calyx fragrant tea and purslane shows excellent antioxidant potential, which significantly reduces the synthesis efficiency of melanin by inhibiting the activity mechanism of tyrosinase, thereby exerting a whitening effect. At the same time, these extracts also have the ability to scavenge free radicals, which helps to increase the moisture content of the skin, not only promotes the soothing of the skin, but also enhances the overall comfort of the skin, and provides strong support for maintaining the healthy state of the skin and stabilizing the skin barrier. The synergistic effect between the two is particularly significant, and its powerful antioxidant performance integrates whitening, moisturizing and deep care functions. When the combined extract of blue calyx fragrant tea and purslane is combined with high-efficiency moisturizing ingredients such as glycerol and trehalose, a more comprehensive moisturizing system is formed. This combination can quickly penetrate into skin cells, promote cell hydration, and give the skin full elasticity and radiance. In addition, the formula also significantly enhances the skin's moisturizing effect, showing excellent repair, moisturizing and soothing effects, indicating that it has broad development potential and application prospects in the skin care market. DETAILED DESCRIPTION

[0010] The above contents of the present invention are further described in detail below through examples, but this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples, and all technologies implemented based on the above contents of the present invention belong to the scope of the present invention.

[0011] Example

[0012] 1. Materials and Methods

[0013] 1.1 Whitening effect test

[0014] Thirty healthy women aged 25-50 with dull skin and easy tanning were selected and randomly divided into 6 groups, with 5 people in each group. After each group of subjects cleaned their skin with warm water, they took 2mL of the test product (combined extract of blue-calyx balsam and purslane, volume ratio 1:1) and applied it on the face, once a day in the morning and evening for 4 weeks. During the experiment, the subjects were not allowed to use any other skin care products on the test area. Before use, on the 7th day, 14th day, 21st day, and 28th day, the highest point of each subject's right cheek was measured using the skin melanin and hemoglobin tester MX18, and the average value was taken for 3 measurements. Test results: Skin melanin content reduction rate = MI Tn -MI T0 / MI T0 *100%, where: MI Tn - Tn value of the tested area, MI T0 - Starting value of the tested area.

[0015] 1.2 Inhibition of tyrosinase activity

[0016] Add 50uL of L-DOPA solution, 50uL of test sample (combined extract of blue-calyx chrysanthemum and purslane, volume ratio 1:1) and 50uL of tyrosinase solution to a 96-well plate, mix well and place in a 30°C water bath for 25min, then test the absorbance A (490nm). Each test was repeated 3 times, and the absorbance of each test was the average of 3 tests to calculate the tyrosinase inhibition rate. The calculation formula of tyrosinase inhibition rate is:

[0017] Tyrosinase inhibition rate = [1-(A x1 -A x2 ,) / (A Y1 -A Y2 )]*100%

[0018] Where:

[0019] A x1 It is the absorbance measured when adding tyrosinase solution and the sample solution to be tested;

[0020] A x2 It is the absorbance measured when the test solution is added but no tyrosinase solution is added (replaced with an equal amount of phosphate buffer solution);

[0021] A Y1 It is the absorbance measured when tyrosinase solution is added but no sample solution is added (replaced with an equal amount of phosphate buffer solution);

[0022] A Y2 It is the absorbance measured when no tyrosinase solution and no sample solution are added (replaced with an equal amount of phosphate buffer solution).

[0023] In the above experiment, the concentration of L-DOPA solution was 1 g / L, prepared with 0.2 mol / L phosphate buffer solution at pH 6.8; the concentration of the test sample was 0.5 g / L, prepared with 0.2 mol / L phosphate buffer solution at pH 6.8; the concentration of the amino acid enzyme solution was 100 U / mL, prepared with 0.2 mol / L phosphate buffer solution at pH 6.8.

[0024] 1.3 Free radical scavenging rate

[0025] The test samples (combined extracts of blue-calyx fragrant tea and purslane, volume ratio 1:1) were prepared into 2wt% test samples with pure water. The T / SHRH006-2018 cosmetics-free radical (DPPH) scavenging test method was used for detection, and the test results were taken as the average of three measurements. The positive control was dissolved and diluted with 95vol% ethanol to 0.08mg / L, 0.04mg / L, 0.02mg / mL, 0.01mg / mL series concentration gradients to verify the test system. Test treatment: water-soluble test substances were diluted with water to multi-level concentration samples, and oil-soluble test substances were diluted with 95vol% ethanol to multi-level concentration samples. Referring to Table 1, 10mL test tubes were used to set up sample tubes (T), sample background (T0), DPPH tubes (C) and solvent background (C0). Three parallel tubes were set up for each sample of each tested concentration (T), and three parallel tubes were also set up for the DPPH tube (C). Add 1 mL of sample solution of the same concentration to the sample tube (T) and sample background (T).

[0026] Table 1 Sample addition requirements

[0027] T-sample tube <![CDATA[T0 - Sample background]]> C-DPPH tube <![CDATA[C0 - Solution background]]> Sample solution (mL) 1 1 - - Water or 95vol% ethanol solution (mL) 2 2 3 3 DPPH ethanol solution (mL) 1 - 1 - 95vol% ethanol (mL) - 1 - 1 Parallel times 3 / Sample 1 / sample 3 / Experimental 1 / Experiment

[0028] DPPH free radical scavenging rate calculation formula:

[0029]

[0030] Where:

[0031] T-sample tube absorbance value is the absorbance value of the solution after the sample reacts with DPPH;

[0032] T0-sample background absorbance;

[0033] The average of the three absorbance values ​​of the C-DPPH tube is the absorbance value of the DPPH solution when no sample is added;

[0034] C0- solution background absorbance.

[0035] 1.4 Moisturizing effect test

[0036] Thirty healthy women aged 25-50 with dull skin and easy tanning were selected and randomly divided into 6 groups, with 5 people in each group. After cleaning the skin with warm water, the subjects in each group took 2mL of the corresponding test product and applied it on the face once in the morning and evening every day for 4 weeks. During the experiment, the subjects were not allowed to use any other skin care products on the test area; before use, on the 7th day, 14th day, 21st day, and 28th day, the moisture tester Cormeometer CM825 was used to measure the water content of the stratum corneum at the highest point of each subject's right cheek. The point-touch measurement was used, and the average value was taken for 3 measurements. Before the test, the subjects washed their faces and rested quietly for 30 minutes. Test results:

[0037] Skin moisture content growth rate % = (MMV t ,-MMV0) / MMV0*100%

[0038] Where:

[0039] MMV0-skin moisture content before use;

[0040] MMV t - Skin moisture content after t days of use.

[0041] 2. Results

[0042] 2.1 Melanin reduction rate test results

[0043] Table 2 Melanin reduction rate

[0044]

[0045] Note: p<0.05

[0046] From Table 2, it can be seen that the melanin of the 6 groups of people was significantly reduced after using the product within 28 days, and the reduction rate was positively correlated with the use time.

[0047] 2.2 Tyrosinase activity inhibition rate test results

[0048] Table 3 Tyrosinase activity inhibition rate

[0049] Grouping Tyrosinase inhibition rate% P-value SD 1 62.42 ＜0.05 5.86 2 67.88 ＜0.05 6.48 3 59.63 ＜0.05 8.63 4 65.49 ＜0.05 5.44 5 66.11 ＜0.05 6.24 6 60.99 ＜0.05 6.71

[0050] It can be seen from the experimental results in Table 3 that the product of the present application has an inhibitory effect on tyrosinase and can more effectively reduce the amount of melanin produced.

[0051] 2.3 Free radical scavenging rate test results

[0052] Table 4 Free radical scavenging rate

[0053]

[0054]

[0055] From the experimental results in Table 4, it can be seen that the product of the application has a significant scavenging effect on free radicals.

[0056] 2.4 Skin moisture content growth rate test results

[0057] Table 5 Moisture content growth rate

[0058]

[0059] Note: P<0.05

[0060] From the experimental results in Table 5, it can be seen that the skin moisture of the six groups of people increased significantly after using the product within 28 days, and the growth rate was positively correlated with the use time.

[0061] In the field of skin care science, the combined extracts of Isodon calyx and Portulaca oleracea have shown excellent antioxidant potential. They significantly reduce the synthesis efficiency of melanin by inhibiting the activity of tyrosinase, thereby exerting a whitening effect. At the same time, these extracts also have the ability to scavenge free radicals, which helps to increase the moisture content of the skin, not only promoting skin soothing, but also enhancing the overall comfort of the skin, providing strong support for maintaining the healthy state of the skin and stabilizing the skin barrier. The synergistic effect between the two is particularly significant, and their powerful antioxidant properties combine whitening, moisturizing and deep care functions.

[0062] When these extracts are combined with highly effective moisturizing ingredients such as glycerin and trehalose, a more comprehensive moisturizing system is formed. This combination can quickly penetrate into skin cells, promote cell hydration, and give the skin full elasticity and luster. In addition, the formula also significantly enhances the moisturizing effect of the skin, showing excellent repair, moisturizing and soothing effects, indicating that it has broad development potential and application prospects in the skin care market.

[0063] The basic principles, main features and advantages of the present invention are shown and described above. Without departing from the spirit and scope of the present invention, the present invention may also be subject to various changes and improvements, which all fall within the scope of the present invention to be protected.

Claims

1. Application of the combined extract of Ipomoea aquatica and Portulaca oleracea in the preparation of skin care products with whitening effect.

2. The use according to claim 1, characterized in that: The combined extract of the blue-calyx schizonepeta and purslane has an inhibitory effect on tyrosinase activity and a scavenging effect on free radicals, thereby achieving a whitening effect.

3. The use according to claim 1, characterized in that: The combined extracts of Ipomoea aquatica and Portulaca oleracea are combined with the highly effective moisturizing ingredients of glycerin and trehalose to form a moisturizing system for hair care products, which can quickly penetrate into skin cells, promote cell hydration, and give the skin full elasticity and luster.

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