Low-temperature preservation device and method for stem borers

Abstract

The invention relates to the technical field of low-temperature preservation, in particular to a stem borer low-temperature preservation device and method.The device comprises a box body, fixing blocks are symmetrically and fixedly connected to the inner side wall of the box body, and a first groove and a second groove are formed in each fixing block. And overturning assemblies are arranged on the inner sides of the fixing blocks, and connecting rods are arranged between the adjacent overturning assemblies. A transmission assembly is arranged at the bottom of the overturning assembly on one side in the box body, a gear is arranged on the side, away from the overturning assembly, of the transmission assembly, the gear is rotationally connected with the fixing block and meshed with a rack, a driving piece is fixedly connected to the inner side wall of the box body, and an output shaft of the driving piece is coaxially and fixedly connected with the gear. The bottom of the rack is fixedly connected with a placing plate, and the bottom of the placing plate is provided with a refrigeration assembly and a lifting assembly. Through the gear, the rack, the placing plate and the telescopic rod, when stem borer pupae need to be stored at low temperature, the gear drives the rack to move, so that the placing plate is located at the bottom of the box body and is closer to a refrigerator, and the refrigerating effect of the stem borer pupae in the box body is improved.

Description

Technical Field

[0001] The invention relates to the technical field of low-temperature preservation, and in particular to a device and method for low-temperature preservation of stem borers. Background Art

[0002] Sugarcane borer belongs to the order Lepidoptera, commonly known as sugarcane borer. Common species include the yellow borer of the leaf roller family, Pyralidae The striped stem borer and the two-spotted stem borer are among the most serious common pests on sugarcane, causing heavy economic losses to my country's sugar industry every year. They are mainly distributed in Guangdong, Guangxi, Yunnan, Hainan, Fujian, Sichuan, Jiangxi and other provinces and regions. In addition to harming sugarcane, they also harm crops such as sorghum and corn.

[0003] Sugarcane borer harms sugarcane throughout its entire growing period. The period and number of sugarcane borer outbreaks vary from sugarcane area to sugarcane area due to differences in altitude, rainfall, cultivation environment, planting system and variety. In most sugarcane areas, multiple types of sugarcane borer exist at the same time. From planting to harvesting, sugarcane is harmed to varying degrees by sugarcane borers, posing a serious threat to sugarcane production.

[0004] The existing low-temperature preservation device for corn borers has poor low-temperature preservation effect when preserving corn borer pupae, and the low-temperature preservation requires a long time of cooling to reach the preservation temperature of corn borer pupae. Therefore, it is necessary to provide a low-temperature preservation device and method for corn borers. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a device and method for low-temperature preservation of corn borer pupae. Through gears, racks, a placement plate and a telescopic rod, when the corn borer pupae need to be preserved at a low temperature, the gears are used to drive the racks to move, so that the placement plate is located at the bottom of the box and is closer to the refrigerator, thereby improving the refrigeration effect of the corn borer pupae in the box.

[0006] In order to achieve the above-mentioned purpose, the technical scheme of the present invention is as follows: a device and method for low-temperature preservation of stem borers, comprising a box body, a controller fixedly connected to the outer wall of the box body, fixed blocks symmetrically fixedly connected to the inner side walls of the box body in both length directions, a vertical first groove and an arc-shaped second groove are formed on the fixed blocks, and the first groove is connected to the second groove.

[0007] An opening is provided on the top of the box body, a top cover is arranged in the opening, a flip assembly for flipping the top cover is arranged on the inner side of the fixed block, and a connecting rod for connecting the flip assemblies is arranged between adjacent flip assemblies.

[0008] A transmission assembly is provided at the bottom of the flip assembly on one side of the box body, and a gear is provided through the fixed block on the side of the transmission assembly away from the flip assembly. The gear is rotatably connected to the fixed block, and the gear is meshed with a rack. A driving member is fixedly connected to the inner wall of the box body, and the output shaft of the driving member is coaxially fixedly connected to the gear. The controller is used to control the start and stop of the driving member.

[0009] A placing plate is fixedly connected to the bottom of the rack, and a refrigeration component for cooling the placing plate and a lifting component for lifting along with the placing plate are arranged at the bottom of the placing plate.

[0010] The technical principle of the above scheme is as follows: when it is necessary to preserve the stem borer pupae at low temperature, the staff can start the drive assembly through the controller to rotate the gear. Through the meshing of the gear and the rack, the rotation of the gear will be converted into the linear motion of the rack. Through the linear motion of the rack, the placement plate fixedly connected to the bottom of the rack will rise and fall with the movement of the rack. At this time, by placing the placement plate close to the refrigerator, the refrigeration effect of the refrigerator can be improved, thereby improving the low-temperature preservation effect of the stem borer pupae.

[0011] The above scheme has the following beneficial effects:

[0012] 1. The present invention realizes the lifting function of the placement plate through the cooperation of the gear, the rack and the placement plate. When the placement plate is lowered to the bottom of the box body and is closer to the refrigerator, the refrigeration effect is significantly improved, thereby ensuring the preservation quality of the stem borer pupae in a low temperature environment.

[0013] 2. The present invention controls the start and shut down of the driving component through the controller, making the operation of the entire device simple and quick. The staff only needs to use the controller to realize the lifting and lowering of the placement plate and the switch of the refrigeration component.

[0014] Furthermore, the flip assembly includes a flip plate, the top of the flip plate is fixedly connected to the top cover, and a plurality of sliding columns are fixedly connected to the side of the flip plate close to the fixed block. The sliding columns are slidably matched with the first groove and the second groove, and both ends of the connecting rod are fixedly connected to the bottom end of the flip plate.

[0015] Beneficial effect: The sliding matching design between the sliding column and the first groove and the second groove ensures the stability of the flip plate during the flipping process. This design prevents the flip plate from deflecting or shaking during the flipping process, thereby ensuring the accurate opening and closing of the top cover.

[0016] Furthermore, the transmission assembly includes a first transmission rod, which is rotatably connected to the connecting rod, a second transmission rod is hingedly connected to one end of the first transmission rod away from the connecting rod, and an end of the second transmission rod away from the first connecting rod is fixedly connected to the gear.

[0017] Beneficial effect: When the driving assembly drives the gear to rotate, the gear will drive the second transmission rod to rotate, and the rotation of the second transmission rod will drive the first transmission rod to rotate. When the first transmission rod rotates, it will drive the flip plate to flip upward, and the sliding column will slide in the first groove and the second groove. At this time, the top cover can be opened under the flipping action of the flip plate.

[0018] When the top cover is opened, the placement plate will slide vertically in the box driven by the rack. The two processes of opening the top cover and rising the placement plate are carried out simultaneously, which greatly improves the operating efficiency of the staff and saves time and energy.

[0019] Furthermore, the refrigeration assembly includes a refrigerator, which is fixedly connected to the bottom of the box body, and the controller is used to control the start and stop of the refrigerator.

[0020] Beneficial effect: The refrigerator is fixedly connected to the bottom of the box. When the placement plate is close to the refrigerator, the refrigerator can quickly reduce the temperature of the placement plate and keep it in a stable low temperature range. This design ensures that the stem borer pupae can be stored in the best low temperature environment and improves the efficiency of low temperature storage.

[0021] The controller controls the start and stop of the refrigerator and adjusts the refrigeration temperature. This precise control enables the refrigerator to reduce energy consumption as much as possible while ensuring the refrigeration effect, thus improving the energy efficiency of the entire device.

[0022] Furthermore, the lifting assembly includes a plurality of telescopic rods, which are all fixedly connected to the bottom of the box body, and ends of the telescopic rods close to the placement plate are all fixedly connected to the placement plate.

[0023] Beneficial effect: The fixed connection between the telescopic rod and the bottom of the box body enhances the stability of the placement board. This design allows the placement board to remain stable during the lifting process, avoiding damage or quality degradation of the stem borer pupae due to shaking or tilting.

[0024] When the rack drives the placement board to move, the telescopic rod can provide a certain supporting force for the placement board, which is also beneficial to improving the stability of the placement board.

[0025] Furthermore, a temperature sensor is fixedly connected to the inner wall of the box, and the controller is used to receive temperature data monitored by the temperature sensor.

[0026] Beneficial effects: The temperature sensor can monitor the temperature inside the box in real time and feed the data back to the controller. The controller can precisely control the refrigerator based on the data, so that the temperature inside the box can quickly reach and stabilize within the preset low temperature range. This precise temperature control helps ensure that the stem borer pupae are stored in the optimal temperature environment, avoiding quality degradation or death due to excessively high or low temperatures.

[0027] Furthermore, a plurality of third grooves are formed on the surface of the placement plate.

[0028] Beneficial effect: The third groove can provide a stable supporting point for the culture dish, reducing the sliding or tilting of the culture dish on the placement plate, thereby affecting the low-temperature storage of the stem borer pupae.

[0029] Furthermore, an atomizer is fixedly connected to the inner wall of the box body, and the atomizer is located above the placement plate.

[0030] Beneficial effects: The atomizer can provide a suitable humidity environment for the borer pupae in the box by spraying fine water mist, reducing the impact of humidity on the hatching of borer pupae.

[0031] Furthermore, the inner wall of the box is covered with a thermal insulation layer.

[0032] Beneficial effect: In a low-temperature storage device, the insulation layer can effectively prevent external heat from entering the box, thereby maintaining a stable low-temperature environment inside the box. By reducing heat transfer, the insulation layer helps maintain the stability of the temperature inside the box.

[0033] Further, a method for low temperature preservation of stem borers comprises:

[0034] Step 1: Select several pupae collected on the same day and place them in several culture dishes covered with 4 to 8 layers of wet sterilized medical gauze and wet sterilized cotton balls. The specification of the culture dishes is 90 mm, and 20 pupae are placed in each dish.

[0035] Step 2: Place the culture dish on the placement plate.

[0036] Step 3: Turn on the refrigerator through the controller to maintain the temperature inside the box at 10℃.

[0037] Step 4: Take out a corresponding number of culture dishes as needed every 5 days and count the number of pupae that have emerged and the number of deaths.

[0038] Beneficial effects: By placing the pupae in a culture dish covered with wet sterilized medical gauze and wet sterilized cotton balls, and then placing them in a low-temperature storage box with an insulation layer, a stable and suitable storage environment is provided for the pupae. This environment helps prevent the pupae from getting moldy, reduces the evaporation of water in the pupae, keeps their body surface moist, and thus improves the survival rate.

[0039] Maintain the temperature in the box at 10°C, which is the ideal temperature range for many insect pupae. The appropriate temperature helps maintain the internal physiological activities of the pupae, promotes their normal development, and thus improves the quality of their emergence.

[0040] By counting the number of pupae that emerged and died, accurate data on survival and emergence rates can be obtained. These data are of great significance for evaluating the effect of cryopreservation, optimizing storage conditions, and studying the laws of insect development.

[0041] Compared with room temperature storage, cryopreservation can significantly prolong the survival time of pupae and reduce the loss caused by improper storage. At the same time, cryopreservation can also help slow down the development of pupae, providing more operation time for experimenters.

[0042] Every 5 days, a corresponding number of culture dishes are taken out for statistics. This regular sampling method helps to observe the development of pupae in different time periods. By comparing the data at different time points, we can understand the changes in the development speed and survival rate of pupae.

[0043] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is an axonometric view of a device for low-temperature preservation of stem borers in an embodiment of the present invention.

[0045] Figure 2 It is a side cross-sectional view of the low-temperature storage device for stem borers in an embodiment of the present invention.

[0046] Figure 3 It is an internal axonometric view of the low-temperature storage device for stem borers in an embodiment of the present invention.

[0047] Figure 4 It is an axonometric view of a fixing plate in a stem borer cryopreservation device according to an embodiment of the present invention.

[0048] Figure 5 It is an axonometric view of the turning plate in the low-temperature storage device for stem borers in an embodiment of the present invention.

[0049] Figure 6 1 is a diagram showing the steps of a method for low-temperature preservation of stem borers in an embodiment of the present invention.

[0050] The figure marks in the drawings of the specification include: 1. box body; 2. top cover; 3. controller; 4. fixing block; 5. flip plate; 6. second transmission rod; 7. first transmission rod; 8. placement plate; 9. telescopic rod; 10. refrigerator; 11. rack; 12. gear; 13. second groove; 14. first groove; 15. sliding column; 16. motor; 17. connecting rod; 18. temperature sensor; 19. third groove. DETAILED DESCRIPTION

[0051] The following is further described in detail through specific implementation methods:

[0052] Embodiment 1:

[0053] As attached Figure 1-Figure 5 As shown: a low-temperature storage device for stem borers, including a box body 1, an outer wall of the box body 1 is fixedly connected to a controller 3 by screws, and fixing blocks 4 are symmetrically fixedly connected to the inner side walls of the box body 1 in both length directions by bolts, and the fixing blocks 4 are each provided with a vertical first groove 14 and an arc-shaped second groove 13, and the first groove 14 is connected to the second groove 13.

[0054] The box body 1 has an opening at the top, and a top cover 2 is arranged in the opening. A flip assembly for flipping the top cover 2 is arranged inside the fixing block 4, and a connecting rod 17 for connecting the flip assemblies is arranged between adjacent flip assemblies.

[0055] like Figure 3 and Figure 4 As shown, a transmission assembly is provided at the bottom of the flip assembly on the left side of the box body 1, and a gear 12 is provided through the fixed block 4 on the side of the transmission assembly away from the flip assembly. The gear 12 is rotatably connected to the fixed block 4, and the gear 12 is meshed with a rack 11. A driving member is fixedly connected to the inner wall of the box body 1 by bolts, and the output shaft of the driving member is coaxially fixedly connected to the gear 12 by bolts. In this embodiment, the driving member is a motor 16, and the controller 3 is used to control the start and stop of the motor 16.

[0056] like Figure 2 As shown, the bottom of the rack 11 is fixedly connected to the placement plate 8 by bolts, and the bottom of the placement plate 8 is provided with a refrigeration component for cooling the placement plate 8 and a lifting component for lifting and lowering with the placement plate 8.

[0057] like Figure 4 and Figure 5 As shown, the flip assembly includes a flip plate 5, the top of the flip plate 5 is fixedly connected to the top cover 2 by bolts, and the side of the flip plate 5 close to the fixed block 4 is fixedly connected to a plurality of sliding columns 15 by bolts, and the sliding columns 15 are slidably matched with the first groove 14 and the second groove 13, and both ends of the connecting rod 17 are fixedly connected to the bottom end of the flip plate 5 by bolts.

[0058] The transmission assembly includes a first transmission rod 7, which is rotatably connected to a connecting rod 17. One end of the first transmission rod 7 away from the connecting rod 17 is hinged to a second transmission rod 6, and one end of the second transmission rod 6 away from the first connecting rod 17 is fixedly connected to the gear 12 by bolts.

[0059] like Figure 2 As shown, the refrigeration assembly includes a refrigerator 10 , which is fixedly connected to the bottom of the box body 1 by screws, and the controller 3 is used to control the start and stop of the refrigerator 10 .

[0060] A temperature sensor 18 is fixedly connected to the inner wall of the box body 1 by screws, and the controller 3 is used to receive temperature data monitored by the temperature sensor 18 .

[0061] The specific implementation process is as follows: after selecting the stem borer pupae that need to be stored at low temperature, the staff can place the stem borer pupae in a culture dish, and then use the box 1 to store them.

[0062] by Figure 3 and Figure 4For example, first, the staff can turn on the motor 16 through the controller 3. After the motor 16 is started, it will drive the gear 12 to rotate. When the gear 12 rotates, it will drive the second transmission rod 6 to rotate. At this time, the second transmission rod 6 will push the first transmission rod 7 to rise. When the first transmission rod 7 rises, it will drive the sliding column 15 to slide upward in the first groove 14, thereby driving the flip plate 5 to rise. At this time, the top cover 2 will also rise driven by the flip plate 5.

[0063] When the upper sliding post 15 slides to the top of the first groove 14, the lower sliding post 15 will slide to the connection between the first groove 14 and the second groove 13, and the second transmission rod 6 will continue to rotate under the drive of the motor 16 to rotate. Figure 4 and Figure 5 For example, under the guidance of the second groove 13, the second transmission rod 6 will continue to push the first transmission rod 7 to move to the right, and the sliding column 15 at the connection between the first groove 14 and the second groove 13 will slide from the first groove 14 to the second groove 13. At this time, the flip plate 5 will rotate, and the rotation of the flip plate 5 will drive the top cover 2 to open.

[0064] by Figure 2 and Figure 3 For example, at the same time, when the gear 12 rotates, it will drive the rack 11 to move up and down linearly. Because the rack 11 is fixedly connected to the placement plate 8, when the rack 11 rises, the placement plate 8 will also rise accordingly.

[0065] In general, when the staff starts the motor 16 through the controller 3, the top cover 2 of the box body 1 will open under the flipping action of the flip plate 5, and the placement plate 8 will rise under the linear motion of the rack 11. At this time, the staff can place the prepared culture dish on the placement plate 8. The opening of the top cover 2 and the rising of the placement plate 8 are linked, which simplifies the staff's process of placing the culture dish, thereby improving the staff's work efficiency.

[0066] When the staff has placed the culture dish, the motor 16 can be started to rotate in reverse, and the motor 16 will drive the gear 12 to rotate in the reverse direction, and the reverse rotation of the gear 12 will drive the second transmission rod 6 to rotate in the reverse direction, and the flip plate 5 will rotate in the reverse direction accordingly, and the top cover 2 will be closed. At the same time, the placement plate 8 will also descend with the reverse rotation of the gear 12, and finally the placement plate 8 will descend to a position close to the refrigerator 10.

[0067] Then the staff can turn on the refrigerator 10 through the controller 3, use the temperature sensor 18 to continuously monitor the temperature change in the box 1, and adjust the power of the refrigerator 10 through the controller 3, so that the temperature in the box 1 is always kept stable, thereby improving the low-temperature preservation effect of the stem borer pupae.

[0068] And when the placement plate 8 is lowered to a position close to the refrigerator 10, the refrigerator 10 can directly act on the placement plate 8, which is beneficial to improve the refrigeration efficiency of the refrigerator 10, so that the stem borer pupae have a better low-temperature preservation effect.

[0069] Embodiment 2:

[0070] like Figure 2 As shown, the difference from the above embodiment is that the lifting assembly includes a plurality of telescopic rods 9, and the telescopic rods 9 are fixedly connected to the bottom of the box body 1 by bolts, and the ends of the telescopic rods 9 close to the placement plate 8 are fixedly connected to the placement plate 8 by bolts.

[0071] The specific implementation process is as follows: because a number of culture dishes are placed on the placement plate 8, the placement plate 8 needs to remain stable at all times during the process of the placement plate 8 rising or falling. The telescopic rod 9 can play a certain supporting role for the placement plate 8, reducing the shaking or tilting caused by the rising or falling of the placement plate 8, thereby improving the safety and stability of the stem borer pupae during the storage process.

[0072] Embodiment 3:

[0073] like Figure 3 As shown, the difference from the above embodiment is that a plurality of third grooves 19 are formed on the surface of the placement plate 8 .

[0074] The specific implementation process is as follows: when the staff places the culture dish on the placement plate 8, the culture dish can be embedded in the third groove 19. The third groove 19 can provide an additional fixing point for the culture dish, reduce the movement or slipping of the culture dish on the placement plate 8, and further improve the safety and stability of the low-temperature storage of the stem borer pupae.

[0075] Embodiment 4:

[0076] The difference from the above embodiment is that an atomizer is fixedly connected to the inner wall of the box body 1 by screws, and the atomizer is located above the placement plate 8.

[0077] The specific implementation process is as follows: when the staff places the stem borer pupae in the culture dish, the stem borer pupae need to be moisturized. As the storage time of the stem borer pupae increases, the humidity on the surface of the stem borer pupae will decrease, and the decrease in humidity will affect the emergence of the stem borer pupae. Therefore, the atomizer fixedly connected by screws in the box 1 can moisturize the stem borer pupae during the low-temperature storage process, thereby improving the emergence rate of the stem borer pupae.

[0078] Embodiment 5:

[0079] The difference from the above embodiment is that the inner wall of the box body 1 is covered with a heat-insulating layer. In this embodiment, polyurethane foam is preferably used as the heat-insulating layer.

[0080] The specific implementation process is as follows: during the refrigeration process of the refrigerator 10, the cold air in the box 1 will continuously exchange heat with the external environment, which will reduce the refrigeration efficiency of the refrigerator 10 for the stem borer pupae. After the inner wall of the box 1 is covered with the insulation layer, the insulation layer can effectively reduce the heat transfer between the inside and outside of the box 1, reduce energy consumption, and improve the insulation performance of the box 1, which is conducive to maintaining the stability of the temperature in the box 1 and improving the low-temperature preservation effect of the stem borer pupae.

[0081] Embodiment 6:

[0082] like Figure 6 As shown, a method for low temperature preservation of stem borers comprises:

[0083] Step 1: Select several pupae collected on the same day and place them in several culture dishes covered with 4 to 8 layers of wet sterilized medical gauze and wet sterilized cotton balls; the specification of the culture dish is 90 mm, and 20 pupae are placed in each dish.

[0084] Step 2: Place the culture dish on the placement plate 8.

[0085] Step 3: Turn on the refrigerator 10 through the controller 3 to maintain the temperature in the box 1 at 10°C.

[0086] Step 4: Take out a corresponding number of culture dishes as needed every 5 days and count the number of pupae that have emerged and the number of deaths.

[0087] The specific implementation process is as follows: the staff needs to collect a certain number of stem borer pupae from the laboratory on the same day, ensuring that these pupae are healthy and complete.

[0088] Then, a culture dish was selected and 6 layers of wet sterilized medical gauze were laid on the bottom of each culture dish. The humidity of the gauze was 75% to maintain the appropriate humidity environment required by the pupae. By using sterilized cotton balls to sterilize the pupae, it is helpful to reduce the occurrence of mold in the long-term storage of the pupae, so that the pupae have a better emergence rate and survival rate, reduce the impact on the experiment, and improve the accuracy of the experiment.

[0089] Then, the top cover 2 is opened, and the culture dish with the stem borer pupae is placed on the placement plate 8, and then the top cover 2 is closed.

[0090] The refrigerator 10 is started by the controller 3 so that the temperature in the box 1 is maintained at 10°C.

[0091] Take out a part of the culture dishes every 5 days to observe the number of emergent and dead stem borer pupae, and then make statistics to calculate the emergence rate and mortality rate of stem borer pupae, and finally determine the storage time of stem borer pupae in low temperature state.

[0092] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A low temperature storage device for stem borers, comprising a housing (1), characterized in that: A controller (3) is fixedly connected to the outer wall of the box body (1); fixed blocks (4) are symmetrically fixedly connected to the inner side walls of the box body (1) in both length directions; the fixed blocks (4) are each provided with a vertical first groove (14) and an arc-shaped second groove (13); the first groove (14) is connected to the second groove (13); The box body (1) has an opening at the top, and a top cover (2) is arranged in the opening; a flip assembly for flipping the top cover (2) is arranged on the inner side of each fixed block (4); and connecting rods (17) for connecting the flip assemblies are arranged between adjacent flip assemblies; A transmission assembly is provided at the bottom of the flip assembly on one side of the box body (1); a gear (12) is provided on the side of the transmission assembly away from the flip assembly and passes through the fixed block (4); the gear (12) is rotationally connected to the fixed block (4); the gear (12) is meshed with a rack (11); a driving member is fixedly connected to the inner wall of the box body (1); an output shaft of the driving member is coaxially fixedly connected to the gear (12); and a controller (3) is used to control the start and stop of the driving member; The bottom of the rack (11) is fixedly connected to a placement plate (8), and the bottom of the placement plate (8) is provided with a refrigeration component for cooling the placement plate (8) and a lifting component for lifting along with the placement plate (8).

2. The stem borer cryopreservation device according to claim 1, characterized in that: The flip assembly comprises a flip plate (5), the top of which is fixedly connected to the top cover (2); a plurality of sliding columns (15) are fixedly connected to the side of the flip plate (5) close to the fixed block (4), the sliding columns (15) are slidably matched with the first groove (14) and the second groove (13), and both ends of the connecting rod (17) are fixedly connected to the bottom end of the flip plate (5).

3. The stem borer cryopreservation device according to claim 2, characterized in that: The transmission assembly comprises a first transmission rod (7), the first transmission rod (7) is rotatably connected to a connecting rod (17), one end of the first transmission rod (7) away from the connecting rod (17) is hingedly connected to a second transmission rod (6), and one end of the second transmission rod (6) away from the first connecting rod (17) is fixedly connected to a gear (12).

4. The stem borer cryopreservation device according to claim 3, characterized in that: The refrigeration assembly comprises a refrigerator (10), the refrigerator (10) is fixedly connected to the bottom of the box body (1), and the controller (3) is used to control the start and stop of the refrigerator (10).

5. The stem borer cryopreservation device according to claim 4, characterized in that: The lifting assembly comprises a plurality of telescopic rods (9), wherein the telescopic rods (9) are all fixedly connected to the bottom of the box body (1), and one end of the telescopic rods (9) close to the placement plate (8) is fixedly connected to the placement plate (8).

6. The stem borer cryopreservation device according to claim 5, characterized in that: A temperature sensor (18) is fixedly connected to the inner wall of the box body (1), and the controller (3) is used to receive temperature data monitored by the temperature sensor (18).

7. The stem borer cryopreservation device according to claim 6, characterized in that: A plurality of third grooves (19) are formed on the surface of the placement plate (8).

8. The stem borer cryopreservation device according to claim 7, characterized in that: An atomizer is fixedly connected to the inner wall of the box body (1), and the atomizer is located above the placement plate (8).

9. The stem borer cryopreservation device according to claim 8, characterized in that: The inner wall of the box body (1) is covered with a thermal insulation layer.

10. A method for low temperature preservation of stem borers, characterized in that: include: Step 1: Select several pupae collected on the same day and place them in several culture dishes covered with 4 to 8 layers of wet sterilized medical gauze and wet sterilized cotton balls; the specification of the culture dish is 90 mm, and 20 pupae are placed in each dish. Step 2: placing the culture dish on the placement plate (8); Step 3: Turn on the refrigerator (10) through the controller (3) to maintain the temperature in the box (1) at 10°C; Step 4: Take out a corresponding number of culture dishes as needed every 5 days and count the number of pupae that have emerged and the number of deaths.

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