Formula food for children as well as preparation method and application of formula food

Abstract

The present invention provides a children formula food and a preparation method and application thereof, the children formula food contains DHA, ARA and nervonic acid composition, the mass ratio of DHA to ARA is 0.2-5: 1, and the mass ratio of the total amount of DHA and ARA to nervonic acid is 0.8-20: 1; wherein the content of total protein is 7.8 g to 30 g / 100 g, the content of fat is 10 g to 35 g / 100 g, and the content of carbohydrate is 40 g to 79.3 g / 100 g on the basis of dry matter of the infant formula food. The children formula food provided by the invention has the effect of effectively improving and / or improving individual social behaviors.

Description

Technical Field

[0001] The present invention relates to the technical field of infant and children's food, and in particular to a pediatric formula food and a preparation method and application thereof. Background Art

[0002] The early stage of life is the fastest period of body development, and the childhood stage is the golden period of healthy development throughout the entire life stage. It is very important to ensure the stable and healthy development of children. Balanced nutrition has the most important impact on healthy development. DHA (docosahexaenoic acid), ARA (arachidonic acid) and neuraminic acid are three important unsaturated fatty acids. DHA is a fatty acid that is mainly found in the brain, nerve tissue and retina. It not only constitutes an important component of these tissues, but also helps to maintain the fluidity and stability of cell membranes. ARA is also an important fatty acid in the brain and participates in the structure and function of nerve cells. As a special fatty acid, neuraminic acid is an important component of brain white matter and is indispensable for the health and development of the nervous system. In the current existing technology, DHA, ARA and neuraminic acid are usually added alone or in combination to infant or children's formula or nutritional products to enhance their nutritional functional value. However, the current effects of DHA, ARA and neuraminic acid on brain function are mostly focused on the effects of a single component, while there are relatively few studies on the combined effects of these components. In addition, there is still a lack of in-depth research on how to optimize the proportion and method of adding these components to achieve the best nutritional effect.

[0003] Social behavior refers to an individual's ability to interact with people around him and to take care of himself, and is included in the evaluation dimensions of WS / T 580-2017 "Developmental Behavior Assessment Scale for Children Aged 0 to 6". Early childhood is a sensitive and critical period for the development of individual social behavior. The development of good social behavior has a long-term impact on the healthy growth and socialization process of children, and is of great significance to the development of other individual adaptability.

[0004] After searching, there are few reports in the prior art on whether diet affects individual social behavior and what kind of impact it has on individual social behavior. Summary of the invention

[0005] In order to solve the above technical problems, the purpose of the present invention is to provide a pediatric formula food and a preparation method and application thereof. The pediatric formula food comprises a composition of DHA, ARA and neuraminic acid, which has the effect of effectively enhancing and / or improving individual social behavior.

[0006] To achieve the above object, the present invention first provides a pediatric formula food, which contains a composition of DHA, ARA and neuraminic acid, wherein the mass ratio of DHA to ARA is 0.2-5:1, and the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-20:1;

[0007] Wherein, based on the dry matter of the pediatric formula food, the total protein content is 7.8g-30g / 100g, the fat content is 10g-35g / 100g, and the carbohydrate content is 40g-79.3g / 100g.

[0008] In the above-mentioned pediatric formula food, preferably, the amount of ARA contained in the pediatric formula food is 10-420 mg / 100 g of food dry matter, calculated based on the amount of DHA, ARA and ARA in the neuraminic acid composition.

[0009] In the above-mentioned pediatric formula food, preferably, the amount of ARA contained in the pediatric formula food is 15-400 mg / 100 g of food dry matter, calculated based on the amount of DHA, ARA and ARA in the neuraminic acid composition.

[0010] In the above-mentioned pediatric formula food, preferably, the pediatric formula food is powdered infant formula food, infant formula liquid milk or powdered children's reconstituted milk powder, wherein the powdered infant formula food includes powdered infant formula food, powdered follow-on infant formula food and powdered toddler formula food, and the infant formula liquid milk includes infant formula liquid milk, follow-on infant formula liquid milk and toddler formula liquid milk.

[0011] More preferably, based on the preparation of 1000 parts by weight of the powdered infant formula food, the raw material composition of the powdered infant formula food includes: 1-7000 parts by weight of raw cow's milk, 1-600 parts by weight of desalted whey powder, 1-500 parts by weight of lactose, 0-500 parts by weight of skim milk powder, 1-400 parts by weight of edible vegetable blending oil, 1-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 1-20 parts by weight of phospholipids, 1-20 parts by weight of vitamins, 0-40 parts by weight of casein, and 1-30 parts by weight of minerals;

[0012] Based on the preparation of 1000 parts by weight of the infant formula liquid milk, the raw material composition of the infant formula liquid milk includes: 1-900 parts by weight of raw cow milk, 1-900 parts by weight of purified water, 0-130 parts by weight of skim milk powder, 1-130 parts by weight of desalted whey powder, 1-130 parts by weight of lactose, 1-40 parts by weight of edible vegetable blending oil, 1-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 1-10 parts by weight of phospholipids, 0-40 parts by weight of casein, 1-20 parts by weight of vitamins, and 1-30 parts by weight of minerals;

[0013] Based on the preparation of 1000 parts by weight of the powdered children's formula milk powder, the raw material composition of the powdered children's formula milk powder includes: 1-7000 parts by weight of raw cow's milk, 1-500 parts by weight of skim milk powder, 0-600 parts by weight of desalted whey powder, 0-120 parts by weight of whey protein powder, 1-500 parts by weight of lactose, 0-200 parts by weight of edible vegetable blending oil, 0-50 parts by weight of prebiotics, 1-5 parts by weight of choline, 1-10 parts by weight of phospholipids, 1-20 parts by weight of vitamins, and 1-30 parts by weight of minerals.

[0014] Further preferably, the mineral is one or a combination of two or more of complex minerals, milk mineral salts and calcium carbonate.

[0015] Further preferably, the prebiotic is one or a combination of two or more of galacto-oligosaccharide, fructo-oligosaccharide, isomerized lactose and fucyl lactose. Prebiotics provide support for intestinal health.

[0016] More preferably, the raw cow's milk is whole-fat raw cow's milk or partially skimmed raw cow's milk. Raw cow's milk provides a basic source of milk protein and fat.

[0017] Further preferably, the desalted whey powder and whey protein powder are whey and / or concentrated whey liquid and / or concentrated whey powder. Desalted whey powder and whey protein powder provide high-quality whey protein.

[0018] Further preferably, the edible vegetable blended oil may contain 1,3-dioleyl-2-palmityl triglyceride. The edible vegetable blended oil provides essential fatty acids.

[0019] Lactose is the main source of carbohydrates. Skim milk powder is the basic source of protein and lactose. Minerals, trace elements and vitamins provide necessary nutrients to maintain the comprehensive nutritional needs of children.

[0020] Further preferably, the raw material for providing the DHA may be DHA oil and / or powder derived from one or a combination of two or more of algae, tuna oil, fish oil, milk-derived DHA from feed nutrition regulation biotransformation, egg yolk extract and krill oil; wherein the algae may be one or a combination of two or more of Schizochytrium, Okenichthys and Crypthecodinium. Unless otherwise specified, the ratio of the DHA to other functional components in the composition of the present invention is based on the content of DHA in the raw material.

[0021] Further preferably, the raw material for providing the ARA can be one or more raw materials derived from algae and other oils and fats containing ARA. Unless otherwise specified, the ratio of the ARA to other functional components in the composition of the present invention is based on the content of ARA in the raw material.

[0022] Further preferably, the raw material for providing the nervonic acid can be one or a combination of two or more of nervonic acid derived from cis-15-tetracosenoic acid, Acer truncatum seed oil, nervonic acid synthesized by microbial fermentation, and other oils containing nervonic acid. Unless otherwise specified, the ratio of the nervonic acid to other functional components in the composition of the present invention is based on the content of nervonic acid in the raw material.

[0023] The present invention also provides the use of the above-mentioned pediatric formula food in preparing a product that helps to enhance and / or improve the social behavior of an individual.

[0024] In the above application, preferably, the enhancing and / or improving individual social behavior includes: enhancing the individual's social will and / or social ability;

[0025] More preferably, the social ability includes social interaction ability and / or social adaptability.

[0026] In the above application, preferably, the individual is an infant, a toddler or a child.

[0027] According to a specific embodiment of the present invention, the infant formula food of the present invention (including powdered infant formula food and / or infant formula liquid milk) has a total protein content of 7.8g-22.8g / 100g in terms of dry matter, whey protein accounts for 40%-75% of the total protein, the fat content is 14.9g-33.8g / 100g, and the carbohydrate content is 40.7g-79.3g / 100g; the powdered children's formula milk powder has a total protein content of 16.5g-30g / 100g, a fat content of 10g-35g / 100g, and a carbohydrate content of 40g-70g / 100g.

[0028] Preferably, the total protein content of the infant formula food converted into dry matter is 10g-18g / 100g; the proportion of whey protein in the total protein is 40%-70%; the fat content is 18g-30g / 100g; and the carbohydrate content is 52g-60g / 100g; the total protein content of the children's formula milk powder converted into dry matter is 17g-25g / 100g, the fat content is 12g-25g / 100g; and the carbohydrate content is 45g-60g / 100g.

[0029] According to a specific embodiment of the present invention, the infant formula food (including powdered infant formula food and / or infant formula liquid milk) of the present invention has a linoleic acid content of 2700-6000 mg / 100 g and an α-linolenic acid content of 270-600 mg / 100 g in terms of dry matter.

[0030] According to a specific embodiment of the present invention, the infant formula food of the present invention includes formula food for infants aged 0-6 months, formula food for older infants aged 6-12 months, and formula food for infants aged 12-36 months; wherein: the formula food for infants aged 0-6 months has a total protein content of 8.5-16 g / 100 g, a whey protein content of 60-75% of the total protein, a fat content of 20.5-32.5 g / 100 g, a linoleic acid content of 2700-6000 mg / 100 g, an α-linolenic acid content of 270-600 mg / 100 g, and a carbohydrate content of 50-60 g / 100 g; the formula food for older infants aged 6-12 months has a dry matter content of 1.5-2.0 g / 100 g, a whey protein content of 1.5-2.0 g / 100 g, a fat content of 2.5-2.0 g / 100 g, a linoleic acid content of 2700-6000 mg / 100 g, an α-linolenic acid content of 270-600 mg / 100 g, and a carbohydrate content of 50-60 g / 100 g. The total protein content of the substance is 10-20g / 100g, the proportion of whey protein in the total protein is 40-75%, the fat content is 20-30g / 100g, the linoleic acid content is 2700-4500mg / 100g, the α-linolenic acid content is 270-450mg / 100g, and the carbohydrate content is 50-60g / 100g; the total protein content of the formula food for infants aged 12-36 months is 10-20g / 100g, the fat content is 20-30g / 100g, the linoleic acid content is 2700-4500mg / 100g, the α-linolenic acid content is 270-450mg / 100g, and the carbohydrate content is 50-60g / 100g.

[0031] The present invention also provides a method for preparing the above-mentioned pediatric formula food, wherein the pediatric formula food comprises powdered infant formula food, infant formula liquid milk or powdered children's formula milk powder, and the preparation method of the powdered infant formula food and powdered children's formula milk comprises the following steps:

[0032] S101: Mixing and preparation: According to the type of pediatric formula food, the corresponding ingredients are mixed in proportion to ensure uniform distribution and stability of the formula to obtain a mixed material; this step ensures that the ingredients are mixed evenly in proportion to ensure the consistency and stability of the formula, so that the nutritional components of each batch of products are balanced and meet the expected nutritional standards;

[0033] S102: Production and processing: The mixed material is homogenized after filtering and removing impurities, the homogenization temperature is 50℃-65℃, the homogenization pressure is 120-200bar, and a homogenized material is obtained. The homogenized material is concentrated, and the dry matter weight content of the concentrate is 40%-60% to obtain a concentrated material. The concentrated material is sterilized at 90℃-100℃ for 10-60s to obtain a sterilized material. The sterilized material is dried by a spray drying tower, with an inlet air temperature of 150℃-200℃, an exhaust air temperature of 80℃-120℃, a negative pressure of -10 to -2mmWG, and a high-pressure pump pressure of 140-250bar, and then introduced into a fluidized bed for secondary drying to obtain a powdered food; this step adopts advanced production processes such as homogenization, pasteurization, spray drying or UHT sterilization to ensure the safety and quality of the product, extend the shelf life, and retain the activity and stability of the nutrients;

[0034] The preparation method of infant formula liquid milk includes the following steps: fresh milk purification, cold storage, mixing in proportion, high-pressure homogenization, volume setting, ultra-high temperature instantaneous sterilization, and aseptic filling. Specifically, the fresh milk is filtered through a membrane and purified and then stored at 2-8°C for use, the ingredients are mixed in proportion at 55-65°C for 10-20 minutes to ensure uniform distribution of the liquid and stability of the formula, the milk is homogenized at 300-350 bar and then cooled to a constant volume, and aseptically filled after ultra-high temperature instantaneous sterilization at 135-150°C for 2-8s.

[0035] The "children" referred to in this manual refer to children between the ages of 0 and 6. According to different age groups and growth and development characteristics, "children" can be further divided into the following stages:

[0036] Infancy (0-1 years old): refers to children from birth to 1 year old.

[0037] Early childhood (1-3 years old): refers to children between 1 and 3 years old.

[0038] Preschool age (3-6 years old): refers to children between 3 and 6 years old.

[0039] In the present invention, unless otherwise specified, the infant refers to an infant of 0-12 months old; the toddler refers to a toddler of 1-3 years old; and the child refers to a child who is 36 months old or older but under 7 years old.

[0040] This definition applies to all parts of the present invention involving "children" to ensure that the technology, products or methods of the present invention meet the safety standards and applicability for children. The design and use parameters of specific products or equipment can be appropriately adjusted according to the characteristics of different age groups to ensure their safety and effectiveness.

[0041] According to some specific embodiments of the present invention, in the present invention, the product can be any of the aforementioned foods, or a food obtained by further processing using the aforementioned pediatric formula food as a raw material without adding or with adding other substances.

[0042] The present invention has the following beneficial technical effects:

[0043] The composition of DHA, ARA and neuraminic acid has a specific mass ratio, wherein the mass ratio of DHA to ARA is 0.2-5:1, and the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-20:1. This ratio combination significantly enhances and / or improves the social behavior of an individual. The enhancement and / or improvement of an individual's social behavior includes: enhancing the individual's social willingness and / or social ability. Social ability includes social interaction ability and / or social adaptability (the ability of an individual to respond to and adapt to the social environment and / or social needs around him or her). This effect is a new application in pediatric formula, which significantly improves the efficiency of nutrient absorption and ensures that children can fully absorb and utilize these key nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 Display the detection results of the convergence frequency of each experimental group.

[0045] Figure 2 Displays the detection results of the approach cumulative time of the experimental examples in each experimental group.

[0046] Figure 3 Displays the test results of the body contact frequency of the experimental examples in each experimental group.

[0047] Figure 4 Displays the test results of the cumulative body contact time of the experimental examples in each experimental group.

[0048] Figure 5 The detection results of the relative movement frequency of the experimental examples in each experimental group are displayed.

[0049] Figure 6 The detection results of the relative movement cumulative time of the experimental examples in each experimental group are displayed. DETAILED DESCRIPTION

[0050] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing the specific embodiments rather than for limiting the scope of protection of the present invention.

[0051] When numerical ranges are given in the embodiments, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected unless otherwise specified in the present invention.

[0052] Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art.

[0053] In addition to the specific methods, equipment, and materials used in the embodiments, based on the understanding of the prior art by those skilled in the art and the description of the present invention, any methods, equipment, and materials of the prior art that are similar or equivalent to the methods, equipment, and materials described in the embodiments of the present invention may also be used to implement the present invention.

[0054] Unless otherwise stated, the experimental methods, detection methods, and preparation methods disclosed in the present invention all adopt conventional techniques in the technical field.

[0055] In each embodiment and experiment, the DHA, ARA and neuraminic acid used are all commercially available food-grade raw materials. Unless otherwise specified, the DHA, ARA and neuraminic acid mentioned in the present invention are measured based on the effective content of the functional components DHA, ARA and neuraminic acid in the raw materials.

[0056] Experimental study on the efficacy of nutritional composition in improving social ability

[0057] 1. Bee species and grouping

[0058] One-day-old Italian honey bees (Apis mellifera) were used, and the intervention methods of different experimental groups were shown in Table 1 based on the diet of feed honey bees.

[0059] Table 1

[0060]

[0061] 2. Basic honey bee training

[0062] 2.1 Diet preparation

[0063] Preparation of diet for the control group: weigh 500 g of sucrose and 10 g of casein hydrolysate, dilute to 1 L with ultrapure water, stir to dissolve, filter and sterilize with a 0.22 μm filter membrane to obtain the 50% (w / v) sucrose solution containing 1% (w / v) casein hydrolysate required for the experiment.

[0064] Diet of treatment groups: The free-feeding group added the treatment reagent to the diet of the control group; the single-fed group was fed with a single concentrated reagent in 5 μL sterile sugar water, with each bee receiving a daily diet of 40 μL sugar water.

[0065] 2.2 Bee picking

[0066] (1) Selection of honeycombs

[0067] Honeycomb spleens of appropriate age were selected from the apiary, quickly transported back to the laboratory, and cultured in an incubator (DHP-9162 constant temperature incubator of Zhongyi Guoke (Beijing) Technology Co., Ltd.) at a temperature of 35°C and a humidity of 60-80%.

[0068] (2) Bee picking

[0069] Starting from the second day, observe the status of the emerging bees every day (if they have not emerged, continue to culture them). Gently remove the emerging bees with tweezers and divide them into disposable transparent culture cups, 30 bees per cup. Divide them into enough cups according to the experimental needs.

[0070] 2.3 Bee pretreatment

[0071] (1) Preparation of intestinal fluid

[0072] The fledged bees were taken directly from the apiary, and the intestines were extracted. Each intestine was ground with 100 μL PBS buffer, and then 50% glycerol was added in a 1:1 ratio and stored at -80°C.

[0073] (2) Colonization of intestinal fluid

[0074] Take a sterile 2mL centrifuge tube, add 990μL sterile PBS buffer, add 10μL worker bee intestinal fluid, mix well, and finally use 50% (w / v) sterile sucrose solution to make up to 2mL mark, shake and mix well. Feed each cup of newly emerged bees with 1 tube of the above mixed solution and continue to culture in a constant temperature incubator for 24h.

[0075] 2.4 Treatment and feeding

[0076] After the colonization of intestinal fluid was completed, treatment feeding began. The CV group was fed with the CV diet, and the treatment group was fed with the treatment diet. Treatment feeding continued for 7 days. During this period, the growth status of the bees was observed daily and the diet was changed in time to ensure that the diet was sufficient. The culture conditions were: dark, 35℃, 60%-80% humidity.

[0077] If the pigs are gavage-fed individually, the diet placed in the feeding cup is CV diet, and only different treatment reagents are added to the gavage-fed diet.

[0078] 3. Experimental testing

[0079] Social skills test:

[0080] (1) Preparation of the behavioral observation room: Turn on the air conditioner and the exhaust fan of the test bench 1-2 hours before the experiment to keep the temperature of the observation room (26°C) constant and to ensure that there is no odor in the test bench.

[0081] (2) Place the experimental bees in groups of three in a transparent, breathable open field that has been prepared in advance.

[0082] (3) The open field was acclimated on the experimental table for 15 minutes, and the open field was filmed from above for 45 minutes.

[0083] (4) The above videos were analyzed using the Etho Vision XT 17 animal social behavior analysis system to obtain animal social behavior ability data, and social behavior was evaluated from two dimensions: social interaction tendency evaluation and social interaction evaluation.

[0084] 4. Experimental Results

[0085] (1) Results of social interaction tendency evaluation

[0086] The evaluation of social interaction tendency is the frequency and time of experimental animals approaching each other but not engaging in social behavior. The present invention uses approach frequency and cumulative approach time to evaluate the ability to tend to socialize (i.e., reflecting the "social willingness" described in the present invention).

[0087] The approaching frequencies of each experimental case are as follows Figure 1 As shown in the figure, compared with Experimental Example 1, there is no significant difference in the approach frequency of Experimental Example 7; the approach frequencies of Experimental Examples 2 and 3 have an increasing trend, but there is no significant difference; the approach frequency indicators of Experimental Examples 4, 5 and 6 have increased significantly. Compared with Experimental Example 3, Experimental Example 6 has an increasing trend, but it is not significant. Compared with Experimental Example 7, the approach frequency of Experimental Example 6 has increased significantly.

[0088] The cumulative approach time of each experimental case is as follows: Figure 2 As shown, compared with Experimental Example 1, there is no significant difference in the approach frequency of Experimental Examples 2-5 and Experimental Example 7, and only the approach cumulative time of Experimental Example 6 is significantly increased; compared with Experimental Examples 3 and Experimental Examples 7, the approach frequency of Experimental Example 6 is significantly increased.

[0089] In summary, in terms of social interaction tendency, at the experimental dose, neuraminic acid intervention alone had no significant effect on approach frequency and cumulative approach time; different proportions of DHA and ARA intervention groups had an effect on approach frequency; combined intervention of DHA, ARA and neuraminic acid significantly increased both approach frequency and cumulative approach time, indicating that the nutritional combination of DHA, ARA and neuraminic acid has an effect on nerve and brain development, promotes social interaction tendency, and has a certain synergistic technical effect on approach frequency and cumulative approach time.

[0090] (2) Social interaction evaluation

[0091] Social interaction evaluation indicates the frequency and duration of actual social interaction of experimental animals. It is the most intuitive expression of an individual's social ability (i.e., reflecting the "social ability" mentioned in the present invention, more specifically mainly including social interaction ability and / or social adaptability). It is evaluated by the frequency of physical contact, the cumulative time of physical contact, the frequency of relative movement, and the cumulative time of relative movement.

[0092] The frequency of physical contact in each experimental case is as follows Figure 3 As shown in Figure 1, there is no significant difference between the groups compared with Experimental Example 1, but there is an increasing trend in Experimental Examples 3, 5, and 6. The cumulative body contact time of each experimental example is shown in Figure 1. Figure 4 As shown, compared with Experimental Example 1, Experimental Example 2, Experimental Example 4, and Experimental Example 5 had no significant differences, and Experimental Example 7 had a significant decrease; Experimental Example 3 had an increasing trend, but no significant difference; Experimental Example 6 had a significant increase in the cumulative time of physical contact. Compared with Experimental Examples 3 and 7, the cumulative time of physical contact in Experimental Example 6 increased significantly.

[0093] The relative movement frequency of each experimental example is as follows Figure 5 As shown in Figure 1, there is no significant difference in Experimental Example 2, Experimental Example 5 and Experimental Example 7 compared with Experimental Example 1; the relative movement frequency of Experimental Example 3, Experimental Example 4 and Experimental Example 6 increased significantly. Compared with Experimental Example 3, Experimental Example 6 has an increasing trend, but it is not significant; compared with Experimental Example 7, the relative movement frequency of Experimental Example 6 increased significantly. The relative movement cumulative time of each experimental example is shown in Figure 1. Figure 6 As shown in the figure, compared with Experimental Example 1, the cumulative relative movement time of Experimental Example 2 and Experimental Example 7 decreased; there was no significant difference between Experimental Example 3, Experimental Example 4 and Experimental Example 5; the cumulative relative movement time of Experimental Example 6 increased significantly. Compared with Experimental Example 3 and Experimental Example 7, the cumulative relative movement time of Experimental Example 6 increased significantly.

[0094] In terms of social interaction indicators, at the experimental dose, intervention with neuraminic acid alone had no significant effect on the frequency of physical contact and the frequency of relative movement, while the cumulative time of physical contact and the cumulative time of relative movement both decreased; intervention groups with different proportions of DHA and ARA had different effects on the frequency of physical contact, the cumulative time of physical contact, the frequency of relative movement and the cumulative time of relative movement, suggesting that the effects of DHA and ARA on social interaction are related to the proportions; after the combined intervention of DHA, ARA and neuraminic acid, the frequency of physical contact, the cumulative time of physical contact, the frequency of relative movement and the cumulative time of relative movement all increased significantly, suggesting that the nutritional combination of DHA, ARA and neuraminic acid has an impact on the development of nerves and brain, and promotes social interaction, and has a certain synergistic technical effect on the social interaction indicators of body contact frequency, cumulative time of body contact, relative movement frequency and cumulative time of relative movement.

[0095] Compared with Experimental Example 1, Experimental Examples 2-5 fortified DHA and ARA in different proportions on the basis of normal diet. The results showed that Experimental Examples 2-5 increased in approach frequency, but only Experimental Examples 4 and 5 had significant differences in increase; there was no significant difference in approach cumulative time in Experimental Examples 2-5, but Experimental Examples 3-5 had an increasing trend; there was no significant difference in body contact frequency and cumulative body contact time in Experimental Examples 2-5, but Experimental Examples 3-5 had an increasing trend; there was no significant difference in relative movement frequency and cumulative relative movement frequency time in Experimental Examples 2, 4 and 5, but the relative movement frequency of Experimental Examples 3-5 had an increasing trend; the cumulative relative movement frequency of Experimental Examples 3 and 5 had an increasing trend. It suggests that different proportions of DHA and ARA have different effects on social behavior. In the experimental examples, the DHA:ARA mass ratio of 1:1 in Experimental Example 3 is the optimal ratio of the two.

[0096] Compared with Experimental Example 1, Experimental Example 7 strengthened neuraminic acid on the basis of a normal diet. The results showed that at the experimental dose and ratio of the present invention, strengthening neuraminic acid alone had no significant effect on the approach frequency, body contact frequency, and relative movement frequency, but could reduce the cumulative approach time, the cumulative body contact time, and the cumulative relative movement time.

[0097] Compared with Experimental Example 1, Experimental Example 6 simultaneously strengthened DHA, ARA and neuraminic acid on the basis of normal diet. The results showed that the intervention in Experimental Example 6 significantly improved social interaction tendency (approach frequency, cumulative approach time) and social interaction (body contact frequency, cumulative body contact time, relative movement frequency and cumulative relative movement time); and compared with Experimental Example 7 in which neuraminic acid was strengthened alone, the approach frequency, cumulative approach time, cumulative body contact time, relative movement frequency and cumulative relative movement time were also significantly increased; compared with the strengthening of DHA and ARA alone, the cumulative approach time, cumulative body contact time and cumulative relative movement time were significantly increased, and the approach frequency, body contact frequency and relative movement frequency all showed an increasing trend.

[0098] According to the above results, the combined intervention of DHA, ARA and neuraminic acid in a specific proportion achieved the optimal nutritional effect on brain nerves and brain functions, and the combined intervention had an increasing effect on approach frequency, cumulative approach time, body contact frequency, cumulative body contact time, relative movement frequency and cumulative relative movement time, indicating that the nutritional combination of DHA, ARA and neuraminic acid has an effect on nerve and brain development, and promotes both social interaction tendency and social interaction, and there is a certain synergistic technical effect.

[0099] Preparation of a pediatric formula comprising a composition for enhancing and / or improving the social behavior of an individual

[0100] Example 1

[0101] This embodiment provides a powdered infant formula food, wherein the total protein content of the powdered infant formula food is 10.5 g / 100 g, the DHA content is 200 mg / 100 g, the ARA content is 200 mg / 100 g, the nervonic acid content is 400 mg / 100 g, the fat content is 27.9 g / 100 g; and the carbohydrate content is 54.6 g / 100 g;

[0102] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0103] 1000 parts of raw milk, 290 parts of desalted whey powder, 230 parts of lactose, 226 parts of edible vegetable blending oil, 40.5 parts of galacto-oligosaccharide, 32 parts of whey protein powder, 17.5 parts of fructo-oligosaccharide, 4 parts of phospholipids, 3.5 parts of complex vitamins and 13 parts of minerals.

[0104] The powdered infant formula food of this embodiment is prepared by a dry-wet composite production process, and the preparation method is as follows: pasteurization of raw milk, batching, mixing, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain a finished product; the specific steps include:

[0105] The raw milk is inspected, roughly filtered, stored after cooling, and placed in a mixing tank after pasteurization (85°C, 15s); the raw materials such as large ingredients and small ingredients are fully mixed in proportion to ensure uniform distribution and formula stability to obtain a mixed material;

[0106] After filtering and removing impurities, the mixed material is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain a homogenous material;

[0107] Subsequently, the homogeneous material is concentrated, and the dry matter weight content of the concentrate is 50%, thereby obtaining a concentrated material;

[0108] The concentrated material is sterilized at 85°C for 15 seconds to obtain a sterilized material;

[0109] The sterilized material is dried by a spray drying tower (inlet air temperature 175°C, exhaust air temperature 100°C, negative pressure -8mmWG, high pressure pump pressure 200bar), and then introduced into a fluidized bed for secondary drying to obtain powdered infant formula.

[0110] After testing, all indicators of the finished product meet the requirements of relevant food standards.

[0111] Example 2

[0112] The present embodiment provides a powdered follow-up infant formula food, wherein the total protein content of the powdered follow-up infant formula food is 12g / 100g, the DHA content is 140mg / 100g, the ARA content is 70mg / 100g, the nervonic acid content is 140mg / 100g, the fat content is 23g / 100g; and the carbohydrate content is 57.6g / 100g;

[0113] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0114] 1000 parts of raw milk, 275 parts of lactose, 225 parts of desalted whey powder, 181 parts of edible vegetable blending oil, 130 parts of skimmed milk powder, 12.5 parts of galacto-oligosaccharides, 15 parts of whey protein powder, 10 parts of casein, 5 parts of oligofructose, 4 parts of phospholipids, 3.5 parts of complex vitamins, and 13 parts of minerals.

[0115] The powdered formula food for older infants in this embodiment is prepared by a dry-wet composite production process, and the preparation method is as follows: pasteurization of raw milk, batching, mixing, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain a finished product;

[0116] The specific steps include:

[0117] The raw milk is inspected, roughly filtered, stored after cooling, and placed in a mixing tank after pasteurization (85°C, 15s); the raw materials such as large ingredients and small ingredients are fully mixed in proportion to ensure uniform distribution and formula stability to obtain a mixed material;

[0118] After filtering and removing impurities, the mixed material is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain a homogenous material;

[0119] Subsequently, the homogeneous material is concentrated, and the dry matter weight content of the concentrate is 50%, thereby obtaining a concentrated material;

[0120] The concentrated material is sterilized at 85°C for 15 seconds to obtain a sterilized material;

[0121] The sterilized material is dried by a spray drying tower (inlet air temperature 175°C, exhaust air temperature 100°C, negative pressure -8mmWG, high pressure pump pressure 200bar), and then introduced into a fluidized bed for secondary drying to obtain powdered larger infant formula.

[0122] After testing, all indicators of the finished product meet the requirements of relevant food standards.

[0123] Example 3

[0124] The present embodiment provides a liquid formula milk for infants, wherein the total protein content in the liquid formula milk for infants is 13.8 g / 100 g in terms of dry matter content, the DHA content is 80 mg / 100 g, the ARA content is 400 mg / 100 g, the nervonic acid content is 24 mg / 100 g, the fat content is 22 g / 100 g; and the carbohydrate content is 55.7 g / 100 g;

[0125] This embodiment is achieved by compounding the following raw materials in parts by weight, and the raw material composition includes (preparing 1000 parts of dry matter):

[0126] 700 parts of raw milk, 400 parts of purified water, 40 parts of desalted whey powder, 17.6 parts of lactose, 30 parts of edible vegetable blending oil, 13.5 parts of galacto-oligosaccharides, 9 parts of whey protein powder, 6 parts of fructo-oligosaccharides, 1 part of casein, 4 parts of phospholipids, 3.5 parts of complex vitamins, and 11 parts of minerals.

[0127] The infant formula liquid milk of this embodiment is prepared by the existing process, and the preparation method is as follows: pasteurization of raw milk, batching, mixing, preheating, homogenization, volume setting, ultra-high temperature instant sterilization, aseptic filling and sealing to obtain the finished product;

[0128] The specific steps include:

[0129] The raw milk is inspected, roughly filtered, stored after cooling, and placed in a mixing tank after pasteurization (85°C, 15s); the large and small ingredients are mixed in proportion at 60°C for 15 minutes to ensure uniform distribution and formula stability, thereby obtaining a mixed material;

[0130] After filtering and removing impurities, the mixed material is homogenized under high pressure and then cooled to constant volume. The homogenization temperature is 50°C and the pressure is 325 bar to obtain a homogenous material.

[0131] Subsequently, the homogenized material is sterilized at 135°C for 5 seconds and then aseptically filled to obtain infant formula liquid milk.

[0132] After testing, all indicators of the finished product meet the requirements of relevant food standards.

[0133] Example 4

[0134] This embodiment provides a powdered children's formula milk powder, wherein the powdered children's formula milk powder has a total protein content of 19g / 100g, a DHA content of 42.5mg / 100g, an ARA content of 85mg / 100g, a nervonic acid content of 8.5mg / 100g, a fat content of 18g / 100g, and a carbohydrate content of 55g / 100g;

[0135] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0136] 4400 parts of raw milk, 210 parts of skimmed milk powder, 200 parts of lactose, 15 parts of galacto-oligosaccharides, 15 parts of calcium carbonate, 7 parts of milk mineral salts, 6 parts of isomerized lactose, 3 parts of complex vitamins, 2 parts of phospholipids, 1 part of complex minerals, and 1 part of choline.

[0137] The powdered children's formula milk powder of this embodiment is prepared by a dry-wet composite production process, and the preparation method is as follows: pasteurization of raw milk, batching, mixing, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain a finished product; the specific steps include:

[0138] The raw milk is inspected, roughly filtered, stored after cooling, and placed in a mixing tank after pasteurization (85°C, 15s); the raw materials such as large ingredients and small ingredients are fully mixed in proportion to ensure uniform distribution and formula stability to obtain a mixed material;

[0139] After filtering and removing impurities, the mixed material is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain a homogenous material;

[0140] Subsequently, the homogeneous material is concentrated, and the dry matter weight content of the concentrate is 50%, thereby obtaining a concentrated material;

[0141] The concentrated material is sterilized at 85°C for 15 seconds to obtain a sterilized material;

[0142] The sterilized material is dried by a spray drying tower (inlet air temperature 175°C, exhaust air temperature 100°C, negative pressure -8mmWG, high pressure pump pressure 200bar), and then introduced into a fluidized bed for secondary drying to obtain powdered children's formula milk powder.

[0143] Upon testing, it was found that various indicators of the finished product met the food standard requirements for children's formula milk powder.

[0144] Example 5

[0145] The present embodiment provides a powdered children's formula milk powder, wherein the powdered children's formula milk powder has a total protein content of 20g / 100g, a DHA content of 60mg / 100g, an ARA content of 20mg / 100g, a nervonic acid content of 100mg / 100g, a fat content of 13.5g / 100g, and a carbohydrate content of 58.5g / 100g;

[0146] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0147] 3400 parts of raw milk, 275 parts of skimmed milk powder, 200 parts of lactose, 100 parts of desalinated whey powder, 12 parts of calcium carbonate, 5 parts of milk mineral salts, 2.5 parts of complex vitamins, 2 parts of phospholipids, 1 part of complex minerals, and 1 part of choline.

[0148] The powdered children's formula milk powder of this embodiment is prepared by a dry-wet composite production process, and the preparation method is as follows: pasteurization of raw milk, batching, mixing, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain a finished product; the specific steps include:

[0149] The raw milk is inspected, roughly filtered, stored after cooling, and placed in a mixing tank after pasteurization (85°C, 15s); the raw materials such as large ingredients and small ingredients are fully mixed in proportion to ensure uniform distribution and formula stability to obtain a mixed material;

[0150] After filtering and removing impurities, the mixed material is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain a homogenous material;

[0151] Subsequently, the homogeneous material is concentrated, and the dry matter weight content of the concentrate is 50%, thereby obtaining a concentrated material;

[0152] The concentrated material is sterilized at 85°C for 15 seconds to obtain a sterilized material;

[0153] The sterilized material is dried by a spray drying tower (inlet air temperature 175°C, exhaust air temperature 100°C, negative pressure -8mmWG, high pressure pump pressure 200bar), and then introduced into a fluidized bed for secondary drying to obtain powdered children's formula milk powder.

[0154] Upon testing, it was found that various indicators of the finished product met the food standard requirements for children's formula milk powder.

[0155] Example 6

[0156] The present embodiment provides a powdered children's formula milk powder, wherein the powdered children's formula milk powder has a total protein content of 21g / 100g, a DHA content of 60mg / 100g, an ARA content of 15mg / 100g, a nervonic acid content of 7.5mg / 100g, a fat content of 20.5g / 100g, and a carbohydrate content of 49.5g / 100g;

[0157] This embodiment is achieved by compounding the following raw materials in parts by weight, and its raw material composition includes (preparing 1000 parts by weight):

[0158] 3100 parts of raw milk, 360 parts of skimmed milk powder, 230 parts of lactose, 80 parts of edible vegetable blending oil, 15 parts of galacto-oligosaccharides, 15 parts of calcium carbonate, 6 parts of isomerized lactose liquid, 5 parts of milk mineral salts, 2.5 parts of complex vitamins, 2 parts of phospholipids, 1 part of complex minerals, and 1 part of choline.

[0159] The powdered children's formula milk powder of this embodiment is prepared by a dry-wet composite production process, and the preparation method is as follows: pasteurization of raw milk, batching, mixing, preheating, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain a finished product; the specific steps include:

[0160] The raw milk is inspected, roughly filtered, stored after cooling, and placed in a mixing tank after pasteurization (85°C, 15s); the raw materials such as large ingredients and small ingredients are fully mixed in proportion to ensure uniform distribution and formula stability to obtain a mixed material;

[0161] After filtering and removing impurities, the mixed material is homogenized at a temperature of 50°C and a pressure of 120 bar to obtain a homogenous material;

[0162] Subsequently, the homogeneous material is concentrated, and the dry matter weight content of the concentrate is 50%, thereby obtaining a concentrated material;

[0163] The concentrated material is sterilized at 85°C for 15 seconds to obtain a sterilized material;

[0164] The sterilized material is dried by a spray drying tower (inlet air temperature 175°C, exhaust air temperature 100°C, negative pressure -8mmWG, high pressure pump pressure 200bar), and then introduced into a fluidized bed for secondary drying to obtain powdered children's formula milk powder.

[0165] Upon testing, it was found that various indicators of the finished product met the food standard requirements for children's formula milk powder.

[0166] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A pediatric formula food comprising a composition of DHA, ARA and neuraminic acid, wherein: The mass ratio of DHA to ARA is 0.2-5:1, and the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-20:1; Wherein, based on the dry matter of the pediatric formula food, the total protein content is 7.8g-30g / 100g, the fat content is 10g-35g / 100g, and the carbohydrate content is 40g-79.3g / 100g.

2. The pediatric formula food according to claim 1, wherein: Calculated based on the amount of DHA, ARA and ARA in the neuraminic acid composition, the amount of ARA contained in the pediatric formula food is 10-420 mg / 100 g of food dry matter.

3. The pediatric formula food according to claim 1, wherein: Calculated based on the amount of DHA, ARA and ARA in the neuraminic acid composition, the amount of ARA contained in the pediatric formula food is 15-400 mg / 100 g of food dry matter.

4. The pediatric formula food according to any one of claims 1 to 3, wherein: The pediatric formula food is powdered infant formula food, infant formula liquid milk or powdered children's reconstituted milk powder, wherein the powdered infant formula food includes powdered infant formula food, powdered follow-on infant formula food and powdered toddler formula food, and the infant formula liquid milk includes infant formula liquid milk, follow-on infant formula liquid milk and toddler formula liquid milk.

5. The pediatric formula food according to claim 4, wherein: Based on the preparation of 1000 parts by weight of the powdered infant formula food, the raw material composition of the powdered infant formula food includes: 1-7000 parts by weight of raw cow milk, 1-600 parts by weight of desalted whey powder, 1-500 parts by weight of lactose, 0-500 parts by weight of skim milk powder, 1-400 parts by weight of edible vegetable blending oil, 1-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 1-20 parts by weight of phospholipids, 1-20 parts by weight of vitamins, 0-40 parts by weight of casein, and 1-30 parts by weight of minerals; Based on the preparation of 1000 parts by weight of the infant formula liquid milk, the raw material composition of the infant formula liquid milk includes: 1-900 parts by weight of raw cow milk, 1-900 parts by weight of purified water, 0-130 parts by weight of skim milk powder, 1-130 parts by weight of desalted whey powder, 1-130 parts by weight of lactose, 1-40 parts by weight of edible vegetable blending oil, 1-100 parts by weight of prebiotics, 0-120 parts by weight of whey protein powder, 1-10 parts by weight of phospholipids, 0-40 parts by weight of casein, 1-20 parts by weight of vitamins, and 1-30 parts by weight of minerals; Based on the preparation of 1000 parts by weight of the powdered children's formula milk powder, the raw material composition of the powdered children's formula milk powder includes: 1-7000 parts by weight of raw cow's milk, 1-500 parts by weight of skim milk powder, 0-600 parts by weight of desalted whey powder, 0-120 parts by weight of whey protein powder, 1-500 parts by weight of lactose, 0-200 parts by weight of edible vegetable blending oil, 0-50 parts by weight of prebiotics, 1-5 parts by weight of choline, 1-10 parts by weight of phospholipids, 1-20 parts by weight of vitamins, and 1-30 parts by weight of minerals.

6. The pediatric formula food according to claim 4, wherein: The powdered infant formula food and / or infant formula liquid milk has a linoleic acid content of 2700-6000 mg / 100 g and an α-linolenic acid content of 270-600 mg / 100 g in terms of dry matter.

7. The method for preparing the pediatric formula food according to any one of claims 1 to 6, wherein: The pediatric formula food includes powdered infant formula food, infant formula liquid milk or powdered children's formula milk powder. The preparation method of the powdered infant formula food and powdered children's formula milk includes the following steps: S101: Mixing and preparation: Mix the corresponding ingredients in proportion according to the type of pediatric formula food to ensure uniform distribution and formula stability to obtain a mixed material; S102: Production and processing: the mixed material is subjected to homogenization after filtering and removing impurities, the homogenization temperature is 50°C-65°C, the homogenization pressure is 120-200bar, and a homogenized material is obtained. The homogenized material is concentrated, and the dry matter weight content of the concentrate is 40%-60% to obtain a concentrated material. The concentrated material is sterilized at 90°C-100°C for 10-60s to obtain a sterilized material. The sterilized material is dried by a spray drying tower, with an inlet air temperature of 150°C-200°C, an exhaust air temperature of 80°C-120°C, a negative pressure of -10 to -2mmWG, and a high-pressure pump pressure of 140-250bar, and then introduced into a fluidized bed for secondary drying to obtain a powdered food; The preparation method of infant formula liquid milk includes the following steps: fresh milk purification, cold storage, mixing in proportion, high-pressure homogenization, volume setting, ultra-high temperature instantaneous sterilization, and aseptic filling. Specifically, the fresh milk is filtered through a membrane and purified and then stored at 2-8°C for use, the ingredients are mixed in proportion at 55-65°C for 10-20 minutes to ensure uniform distribution of the liquid and stability of the formula, the milk is homogenized at 300-350 bar and then cooled to a constant volume, and aseptically filled after ultra-high temperature instantaneous sterilization at 135-150°C for 2-8s.

8. Use of the pediatric formula food according to any one of claims 1 to 6 in the preparation of a product that helps to enhance and / or improve the social behavior of an individual.

9. The use according to claim 8, wherein: The said enhancing and / or improving individual social behavior includes: enhancing individual social willingness and / or social ability; Preferably, the social ability includes social interaction ability and / or social adaptability.

10. The use according to claim 8 or 9, wherein: The individual is an infant, toddler or child.

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