Formula of nutritional diet rich in antioxidants for preventing kidney aging

Abstract

The invention relates to the field of functional food and nutritional health-care products, and discloses an antioxidant-rich nutritional diet formula for preventing kidney aging, the formula comprises the following components by weight: antioxidant components: green tea polyphenol, resveratrol and vitamin E, the total content of which is 5-20%; anti-inflammatory components: curcumin and Omega-3 fatty acid, and the total content of the curcumin and the Omega-3 fatty acid is 3%-15%; the microcirculation regulating component comprises pomegranate extract and flavonoid compounds, and the total content of the pomegranate extract and the flavonoid compounds is 2%-10%; a metabolic optimization component: low-phosphorus vegetable protein, the total content of which is 20%-40%; and intestinal and kidney regulating components: probiotic ferment powder and inulin, the total content of which is 15-30%, and comprehensive kidney health protection is realized through the collaborative design of multifunctional modules. Green tea polyphenol, resveratrol and vitamin E in the antioxidant components can efficiently scavenge free radicals, the antioxidant capacity (T-AOC) reaches 8.5 mmol TE / g, the free radical scavenging rate exceeds 90%, and oxidative stress injuries of renal tubules and glomerulus are remarkably relieved.

Description

Technical Field

[0001] The invention relates to the technical field of functional foods and nutritional health products, and specifically to a nutritional dietary formula rich in antioxidants for preventing kidney aging. Background Art

[0002] With the global population aging, kidney health issues have become an important factor affecting the quality of life of the elderly. The kidney is an important metabolic and detoxification organ of the human body. With age, its function gradually deteriorates, manifested as a series of problems such as renal tubular and glomerular damage, increased metabolic burden, and microcirculatory disorders. Kidney aging is closely related to oxidative stress and chronic inflammation, while the accumulation of free radicals and increased levels of inflammatory factors further accelerate the deterioration of kidney function. Therefore, designing a nutritional intervention program that can comprehensively prevent kidney aging has important scientific value and broad market demand.

[0003] In the existing technology, some dietary supplements or functional foods for kidney health have appeared on the market, but most products have a single function and often only focus on anti-oxidation or basic metabolic support. Some products are centered on a single ingredient, such as green tea polyphenols, resveratrol or probiotics, and fail to achieve multifunctional synergy; some products have added multiple ingredients, but because they have not been scientifically optimized, there may be conflicts between the ingredients. In addition, the processing technology of existing products generally lacks technical treatment for the stability and bioavailability of active ingredients, resulting in a large loss of activity of functional ingredients during storage or use, affecting the actual effect.

[0004] However, current technologies have not yet been able to effectively solve the multiple mechanisms associated with kidney aging, especially nutritional dietary formulas that provide comprehensive protection against oxidative stress, chronic inflammation, metabolic burden and renal microcirculatory disorders through multifunctional synergistic design are still in a research and development gap. At the same time, existing products also have significant deficiencies in the stability of functional ingredients, resulting in the inability to sustain the effects of kidney health interventions. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a nutritional dietary formula rich in antioxidants for preventing kidney aging, which solves the problem that existing products also have significant deficiencies in the stability of functional ingredients, resulting in the inability to sustain the effect of kidney health intervention.

[0006] To achieve the above objectives, the present invention is implemented by the following technical scheme: a nutritional dietary formula rich in antioxidants for preventing kidney aging, the formula comprising the following ingredients in percentage by weight: Antioxidant ingredients: green tea polyphenols, resveratrol and vitamin E, with a total content of 5% to 20%; Anti-inflammatory ingredients: Curcumin and Omega-3 fatty acids, total content 3% to 15%; Microcirculation regulating ingredients: pomegranate extract and flavonoids, with a total content of 2% to 10%; Metabolism-optimizing ingredients: Low-phosphorus plant protein, 20% to 40% total; Intestinal and kidney regulating ingredients: probiotic fermentation powder and inulin, with a total content of 15% to 30%.

[0007] Preferably, the main active ingredient of the green tea polyphenols is EGCG, which accounts for 30% to 60% of the antioxidant module.

[0008] Preferably, the curcumin is prepared by a microwave-assisted extraction process, and the extraction conditions include a power of 300 to 500 W, a solvent of 50% to 70% ethanol, and an extraction time of 20 to 40 minutes.

[0009] Preferably, the resveratrol is obtained by supercritical CO2 extraction, and the extraction conditions include a pressure of 25 to 35 MPa, a temperature of 40 to 50° C., and an extraction time of 40 to 60 minutes.

[0010] Preferably, the probiotic fermentation powder contains lactobacillus and bifidobacterium, the fermentation matrix is ​​inulin, and the fermentation conditions are 37° C., a rotation speed of 100 rpm, and a fermentation time of 24 hours.

[0011] Preferably, the phosphorus content of the low-phosphorus plant protein is less than 1.5 g / kg, which is used to reduce the metabolic burden on the kidneys.

[0012] Preferably, the pomegranate extract is prepared by freeze drying, and after freeze drying, it is dissolved and extracted with pure water, and the extraction conditions are liquid-solid ratio of 10 to 15:1, temperature of 40 to 60° C., and time of 2 to 4 hours.

[0013] Preferably, the flavonoid compound is derived from a plant extract, preferably quercetin, and the extraction process thereof adopts a water-ethanol mixed solvent extraction.

[0014] A method for preparing a nutritional dietary formula rich in antioxidants for preventing kidney aging comprises the following steps: Step 1, extracting antioxidant components, wherein green tea polyphenols are extracted by water-ethanol solvent method, and resveratrol is extracted by supercritical CO2 extraction method; Step 2, extracting anti-inflammatory ingredients, wherein curcumin is extracted by microwave-assisted extraction; Step 3, extracting microcirculation regulating components, wherein the pomegranate extract is freeze-dried; Step 4: preparing an intestinal and kidney regulating component, wherein the probiotic fermentation matrix is ​​inulin; Step 5: Mix the ingredients in proportion to prepare any one of instant powder, sustained-release capsules and functional drinks.

[0015] Preferably, the formula exists in three forms of instant powder, sustained-release capsules and liquid drinks, which can be selected for use according to the needs of different groups of people, and the active stability retention rate of the antioxidant components in each form is greater than 90%.

[0016] The present invention provides a nutritional dietary formula rich in antioxidants for preventing kidney aging. It has the following beneficial effects: 1. The present invention achieves comprehensive kidney health protection through the collaborative design of multifunctional modules. Green tea polyphenols (EGCG), resveratrol and vitamin E in the antioxidant ingredients can efficiently scavenge free radicals, with an antioxidant capacity (T-AOC) of 8.5mmolTE / g and a free radical scavenging rate of more than 90%, significantly reducing oxidative stress damage to renal tubules and glomeruli. Curcumin and Omega-3 fatty acids in the anti-inflammatory module significantly inhibit the release of inflammatory factors TNF-α and IL-6, which were reduced by 60.8% and 50.6% respectively in cell experiments; in animal experiments, the levels of systemic inflammatory factors (TNF-α, IL-6 and CRP) were significantly reduced. The microcirculation regulation module improves glomerular blood flow and microcirculation density through pomegranate extract and flavonoids. Experimental data show that blood flow increased by 43.6%, significantly improving the kidney blood supply environment.

[0017] 2. The low-phosphorus plant protein in the metabolism optimization module of the present invention significantly reduces the metabolic burden on the kidneys. In animal experiments, serum creatinine was reduced by 28%, urea nitrogen was reduced by 22%, and the glomerular filtration rate (GFR) was increased by 15%. Supercritical CO2 extraction, microwave-assisted extraction and freeze-drying were used in the preparation of the formula, which not only improved the purity and activity of resveratrol, curcumin and pomegranate extracts, but also ensured the stability of antioxidant ingredients in instant powders, sustained-release capsules and functional drinks through microencapsulation and freeze-drying processes, with an activity stability retention rate of more than 90%. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The figure is a flow chart of the method of the present invention. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Embodiment 1: Please refer to the attached Figure 1 The embodiment of the present invention provides a nutritional dietary formula rich in antioxidants for preventing kidney aging, the formula comprising the following ingredients in percentage by weight: Antioxidant ingredients: green tea polyphenols, resveratrol and vitamin E, with a total content of 5% to 20%; Anti-inflammatory ingredients: Curcumin and Omega-3 fatty acids, total content 3% to 15%; Microcirculation regulating ingredients: pomegranate extract and flavonoids, with a total content of 2% to 10%; Metabolism-optimizing ingredients: Low-phosphorus plant protein, 20% to 40% total; Intestinal and kidney regulating ingredients: probiotic fermentation powder and inulin, with a total content of 15% to 30%.

[0021] The main active ingredient of green tea polyphenols is EGCG, which accounts for 30% to 60% of the antioxidant module.

[0022] Curcumin is obtained through a microwave-assisted extraction process, and the extraction conditions include a power of 300 to 500 W, a solvent of 50% to 70% ethanol, and an extraction time of 20 to 40 minutes.

[0023] Resveratrol is produced by supercritical CO2 extraction, and the extraction conditions include a pressure of 25 to 35 MPa, a temperature of 40 to 50° C., and an extraction time of 40 to 60 minutes.

[0024] The probiotic fermentation powder contains lactobacillus and bifidobacterium, the fermentation matrix is ​​inulin, and the fermentation conditions are 37°C, a rotation speed of 100 rpm, and a fermentation time of 24 hours.

[0025] The phosphorus content of low-phosphorus plant protein is less than 1.5g / kg, which is used to reduce the metabolic burden on the kidneys.

[0026] The pomegranate extract is prepared by freeze drying, and then dissolved and extracted with pure water. The extraction conditions are liquid-solid ratio of 10 to 15:1, temperature of 40 to 60°C, and time of 2 to 4 hours.

[0027] The flavonoids are derived from plant extracts, preferably quercetin, and the extraction process thereof adopts water-ethanol mixed solvent extraction.

[0028] In one embodiment, the preparation of the antioxidant component Green Tea Polyphenols (EGCG): Using dried green tea leaves as raw materials, according to the water-ethanol mixed solvent extraction process, using 70% ethanol solution, liquid-to-solid ratio 12:1 (v / w), extraction temperature 85°C, extraction time 90 minutes, centrifugation to collect the extract. The extract was concentrated by rotary evaporation to obtain green tea polyphenol powder with an EGCG content of 50%.

[0029] Output: 200g of green tea polyphenol extract.

[0030] Resveratrol: Using Polygonum cuspidatum root as raw material, supercritical CO2 extraction method was adopted, and the process parameters were pressure 30MPa, temperature 45℃, and extraction time 60 minutes. After rotary evaporation and freeze drying, resveratrol extract was obtained with a purity of 95%.

[0031] Output: Resveratrol extract 100g.

[0032] Vitamin E: Purchase food-grade α-tocopherol raw materials from the market and add them directly.

[0033] Preparation of anti-inflammatory ingredients Curcumin: Curcumin was prepared by microwave-assisted extraction using turmeric root powder. The extraction process parameters were as follows: microwave power 400 W, solvent 60% ethanol, liquid-solid ratio 10:1 (v / w), extraction time 30 min, filtration of the extract, rotary evaporation concentration, and freeze drying to obtain curcumin powder with a purity of 90%.

[0034] Output: Curcumin extract 150g.

[0035] Omega-3 fatty acids: Purchase high-purity EPA / DHA fish oil raw materials from the market and add them directly.

[0036] Preparation of microcirculation regulating ingredients 1. Pomegranate Extract: Dried pomegranate peel was selected as raw material, and after freeze-drying, pure water was added for extraction, with a liquid-to-solid ratio of 12:1 (v / w), an extraction temperature of 50°C, and a time of 3 hours. The extract was concentrated and spray-dried to obtain pomegranate polyphenol powder with a purity of 80%.

[0037] Output: 120g of pomegranate polyphenol extract.

[0038] Flavonoids (Quercetin): Sophora japonica seeds were used as raw materials, and a water-ethanol mixed solvent extraction method was adopted, with a solvent ratio of 70%, an extraction temperature of 75°C, and a time of 2 hours. The extract was filtered, concentrated, and then freeze-dried to obtain quercetin powder with a purity of 85%.

[0039] Output: 100g of flavonoid extract.

[0040] Preparation of Metabolism Optimizing Ingredients Low-phosphorus vegetable protein: Use low-phosphorus soy protein powder (phosphorus content less than 1.5g / kg) and add it directly.

[0041] Preparation of enterorenal regulatory components Probiotic baking powder: The probiotic strains are selected from lactobacillus and bifidobacterium, and inulin is used as the matrix for fermentation. The fermentation conditions are: 37°C, 100 rpm, and 24 hours. The fermentation liquid is spray-dried to obtain probiotic fermentation powder, which contains 1 billion CFU per gram of live bacteria.

[0042] Output: 300g probiotic fermentation powder.

[0043] Mixing and preparation of nutritional dietary formulas Recipe ratio: Antioxidant ingredients: 15% of the total formula weight, including 8% green tea polyphenols, 4% resveratrol and 3% vitamin E.

[0044] Anti-inflammatory ingredients: 10% by weight of the total formula, including 6% curcumin and 4% omega-3 fatty acids.

[0045] Microcirculation regulating ingredients: 8% of the total formula weight, of which pomegranate extract accounts for 5% and flavonoids account for 3%.

[0046] Metabolism Optimizing Ingredients: Low-phosphorus plant protein makes up 40% of the total formula weight.

[0047] Intestinal and kidney regulating ingredients: 25% of the total weight of the formula, of which probiotic fermentation powder accounts for 20% and inulin accounts for 5%.

[0048] Mixing and Preparation: Instant powder: The above ingredients are accurately weighed according to the proportion, evenly mixed, and processed through vacuum freeze-drying technology to finally prepare a highly soluble instant powder.

[0049] Extended-release capsules: Resveratrol and curcumin are encapsulated by chitosan microencapsulation technology to form sustained-release particles. The sustained-release particles are mixed with other ingredients in proportion and then filled into capsules, with a net weight of 500 mg per capsule.

[0050] Energy drinks: The pomegranate extract, probiotic fermentation liquid and low-phosphorus plant protein are mixed in proportion, homogenized and then bottled and sterilized.

[0051] Embodiment 2: This embodiment provides a method for preparing a nutritional dietary formula rich in antioxidants for preventing kidney aging based on the above embodiment, comprising the following steps: Step 1, extracting antioxidant components, wherein green tea polyphenols are extracted by water-ethanol solvent method, and resveratrol is extracted by supercritical CO2 extraction method; Step 2, extracting anti-inflammatory ingredients, wherein curcumin is extracted by microwave-assisted extraction; Step 3, extracting microcirculation regulating components, wherein the pomegranate extract is freeze-dried; Step 4: preparing an intestinal and kidney regulating component, wherein the probiotic fermentation matrix is ​​inulin; Step 5: Mix the ingredients in proportion to prepare instant powder, sustained-release capsules or functional drinks.

[0052] The formula exists in three forms: instant powder, sustained-release capsules and liquid drinks, which can be selected according to the needs of different groups of people, and the active stability retention rate of the antioxidant ingredients in each form is greater than 90%.

[0053] Experimental comparison: 1. Antioxidant capacity verification 1.1 Free radical scavenging ability test Purpose of the experiment: To verify the scavenging ability of the dietary formula on DPPH, ABTS and superoxide anion free radicals.

[0054] Experimental methods: DPPH free radical scavenging experiment: Prepare 0.2 mmol / L DPPH ethanol solution.

[0055] The dietary formula extracts were diluted to 50, 100, and 200 μg / mL, respectively.

[0056] Each group of samples was reacted with DPPH solution for 30 min, and the absorbance at 517 nm was measured.

[0057] ABTS free radical scavenging experiment: ABTS+ solution was used, the sample was mixed with the solution for 6 minutes, and the absorbance at 734 nm was measured.

[0058] Superoxide anion free radical scavenging experiment: Superoxide anion free radicals were generated using the self-oxidation method, and the absorbance change was measured after adding the sample.

[0059] Experimental data: Sample concentration (μg / mL) DPPH clearance rate (%) ABTS clearance rate (%) Superoxide anion clearance rate (%) 50 72.5 ± 2.3 68.3 ± 2.1 65.8 ± 1.9 100 85.6 ± 2.7 83.2 ± 2.4 80.1± 2.0 200 95.3 ± 2.0 92.7 ± 2.5 89.6 ± 1.8 1.2 Total antioxidant capacity (T-AOC) test Experimental methods: Use a commercial T-AOC detection kit according to the instructions.

[0060] The sample concentration was 1 mg / mL and the results were expressed as Trolox equivalent (mmol TE / g).

[0061] Experimental data: sample T-AOC (mmol TE / g) Meal formula 8.5 ± 0.3 Comparative antioxidants 9.0 ± 0.2 2. Verification of anti-inflammatory ability 2.1 Cell model experiments Experimental purpose: To verify the inhibitory effect of dietary formula on the release of inflammatory factors (TNF-α, IL-6).

[0062] Experimental methods: LPS (1 μg / mL) was added to RAW 264.7 cells to induce inflammatory response.

[0063] Dietary formula extracts were added at concentrations of 10, 50, and 100 μg / mL.

[0064] After culturing for 24 h, the cell supernatant was collected and the concentrations of TNF-α and IL-6 were detected using ELISA kits.

[0065] Experimental data: Sample concentration (μg / mL) TNF-α reduction rate (%) IL-6 reduction rate (%) 10 25.3 ± 2.1 20.5 ± 2.0 50 40.2 ± 2.3 35.1 ± 2.2 100 60.8 ± 2.5 50.6 ± 2.3 2.2 Animal inflammation model test Experimental purpose: To verify the effect of dietary formula on improving systemic inflammatory response.

[0066] Experimental methods: Grouping and model building: Groups: control group, LPS-induced inflammation model group, and dietary formula treatment group (100 mg / kg).

[0067] The mouse systemic inflammation model was established by intraperitoneal injection of LPS.

[0068] Interventions: The formula-treated group was gavaged with dietary formula solution (100 mg / kg) every day for 7 consecutive days.

[0069] Detection indicators: Serum TNF-α, IL-6, and C-reactive protein (CRP) concentrations.

[0070] Experimental data: index Model Group Recipe Processing Group Improvement rate (%) TNF-α(pg / mL) 85.2 ± 4.3 38.4 ± 3.1 55.0 IL-6 (pg / mL) 72.1 ± 3.5 37.5 ± 2.8 48.0 CRP (mg / L) 28.5 ± 1.8 17.1 ± 1.2 40.0 3. Animal model experiments 3.1 Oxidative stress and renal function protection experiment Experimental purpose: To verify the effect of dietary formula on improving renal function.

[0071] Experimental methods: Grouping and intervention: Control group: given normal feed.

[0072] Model group: renal senescence was induced by injection of D-galactose (150 mg / kg).

[0073] Formula groups: low-dose (200 mg / kg) and high-dose (500 mg / kg) dietary formula were administered daily by gavage.

[0074] Detection indicators: Serum creatinine (SCr), blood urea nitrogen (BUN), malondialdehyde (MDA), and superoxide dismutase (SOD).

[0075] Group SCr(μmol / L) BUN (mmol / L) MDA (nmol / mg) SOD(U / mg) Control group 25.6 ± 2.1 7.8 ± 0.5 1.3 ± 0.1 89.5 ± 3.2 Model Group 52.3 ± 3.2 18.4 ± 1.1 3.8 ± 0.2 45.1 ± 2.4 Low dose group 41.2 ± 2.8 14.6 ± 0.8 2.5 ± 0.2 61.3 ± 2.8 High dose group 37.5 ± 2.4 13.1 ± 0.7 2.1 ± 0.1 65.8 ± 3.0 3.2 Renal microcirculation improvement experiment Experimental purpose: To verify the effect of dietary formula on improving renal microcirculation.

[0076] Experimental methods: Laser Doppler flowmetry was used to detect changes in blood flow in the glomerular region.

[0077] Experimental data: Group Glomerular blood flow (mL / min) Improvement rate (%) Control group 1.25 ± 0.05 - Model Group 0.78 ± 0.03 - Low dose group 1.04 ± 0.04 33.3 High dose group 1.12 ± 0.05 43.6 4. Clinical trials 4.1 Basic information of subjects Experimental population: 60 healthy volunteers, aged 50 to 70 years old, divided into control group and formula group (30 people in each group).

[0078] Intervention duration: 90 days.

[0079] 4.2 Test Results Renal function index: index Changes in the control group Recipe Group Changes Difference rate (%) GFR No change ↑15% +15 SCr No change ↓10% -10 BUN No change ↓12% -12 Antioxidant and inflammatory markers: index Changes in the control group Recipe Group Changes Difference rate (%) MDA No change ↓20% -20 GSH No change ↑25% +25 CRP No change ↓18% -18 IL-6 No change ↓20% -20 This example is aimed at the functional verification of the "nutritional dietary formula rich in antioxidants for the prevention of renal aging". Based on cell models, animal experiments and clinical trials, it is comprehensively verified from multiple dimensions such as anti-oxidation, anti-inflammation, renal function protection and microcirculation improvement. The results fully demonstrate the significant effect of the formula in preventing renal aging: Excellent antioxidant capacity: Free radical scavenging experiments showed that the formula had a scavenging rate of over 89% for DPPH, ABTS and superoxide anion radicals.

[0080] The total antioxidant capacity (T-AOC) reached 8.5 mmol TE / g, which is comparable to commercially available high-efficiency antioxidants.

[0081] Significant anti-inflammatory effect: In the cell model, it effectively inhibited the release of TNF-α and IL-6, with the highest inhibition rates reaching 60.8% and 50.6%, respectively.

[0082] In the animal inflammation model, the formula-treated group significantly reduced serum inflammatory factors (TNF-α decreased by 55%, IL-6 decreased by 48%, and CRP decreased by 40%), indicating its regulatory effect on systemic inflammatory response.

[0083] Renal function improved significantly: In the D-galactose-induced renal aging model, the formula significantly reduced serum creatinine (28%) and urea nitrogen (22%), and improved the oxidative stress state of renal tissue (MDA decreased by 32%, SOD activity increased by 45%).

[0084] The formula also effectively increases glomerular blood flow, with the highest improvement rate reaching 43.6%, reflecting its protective effect on renal microcirculation.

[0085] Clinical trial verification: After 90 days of continuous intervention in healthy people, the formula significantly improved renal function-related indicators (GFR increased by 15%, SCr decreased by 10%, BUN decreased by 12%), while enhancing antioxidant capacity (MDA decreased by 20%, GSH increased by 25%) and reducing inflammatory factor levels (CRP decreased by 18%, IL-6 decreased by 20%).

[0086] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nutritional dietary formula rich in antioxidants for preventing kidney aging, characterized in that: The formulation contains the following ingredients in weight percentage: Antioxidant ingredients: green tea polyphenols, resveratrol and vitamin E, with a total content of 5% to 20%; Anti-inflammatory ingredients: Curcumin and Omega-3 fatty acids, total content 3% to 15%; Microcirculation regulating ingredients: pomegranate extract and flavonoids, with a total content of 2% to 10%; Metabolism-optimizing ingredients: Low-phosphorus plant protein, 20% to 40% total; Intestinal and kidney regulating ingredients: probiotic fermentation powder and inulin, with a total content of 15% to 30%.

2. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The main active ingredient of green tea polyphenols is EGCG, which accounts for 30% to 60% of the antioxidant module.

3. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The curcumin is prepared by a microwave-assisted extraction process, and the extraction conditions include a power of 300 to 500 W, a solvent of 50% to 70% ethanol, and an extraction time of 20 to 40 minutes.

4. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The resveratrol is prepared by supercritical CO2 extraction, and the extraction conditions include a pressure of 25 to 35 MPa, a temperature of 40 to 50° C., and an extraction time of 40 to 60 minutes.

5. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The probiotic fermentation powder contains lactobacillus and bifidobacterium, the fermentation matrix is ​​inulin, and the fermentation conditions are 37° C., a rotation speed of 100 rpm, and a fermentation time of 24 hours.

6. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The phosphorus content of the low-phosphorus plant protein is less than 1.5 g / kg, and is used to reduce the metabolic burden on kidneys.

7. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The pomegranate extract is prepared by freeze drying, and after freeze drying, it is dissolved and extracted with pure water, and the extraction conditions are liquid-solid ratio of 10 to 15:1, temperature of 40 to 60° C., and time of 2 to 4 hours.

8. The antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 1, characterized in that: The flavonoid compound is derived from plant extracts, preferably quercetin, and the extraction process thereof adopts water-ethanol mixed solvent extraction.

9. A method for preparing a nutritional dietary formula rich in antioxidants for preventing kidney aging, according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step 1, extracting antioxidant components, wherein green tea polyphenols are extracted by water-ethanol solvent method, and resveratrol is extracted by supercritical CO2 extraction method; Step 2, extracting anti-inflammatory ingredients, wherein curcumin is extracted by microwave-assisted extraction; Step 3, extracting microcirculation regulating components, wherein the pomegranate extract is freeze-dried; Step 4: preparing an intestinal and kidney regulating component, wherein the probiotic fermentation matrix is ​​inulin; Step 5: Mix the ingredients in proportion to prepare any one of instant powder, sustained-release capsules and functional drinks.

10. The method for preparing the antioxidant-rich nutritional dietary formula for preventing kidney aging according to claim 9, characterized in that: The formula exists in three forms: instant powder, sustained-release capsules and liquid drinks, which can be selected for use according to the needs of different groups of people, and the active stability retention rate of the antioxidant components in each form is greater than 90%.

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