Probiotic composition with digestion promoting effect, preparation method and application

Abstract

The invention discloses a probiotic composition with a digestion promoting effect as well as a preparation method and application thereof. The probiotic composition is prepared from the following raw materials in parts by weight: 30 to 50 parts of lactobacillus plantarum, 10 to 30 parts of lactobacillus rhamnosus, 20 to 40 parts of lactobacillus paracasei, 10 to 30 parts of Grignard bacteria, 20 to 30 parts of chlorella protein, 10 to 20 parts of hyaluronic acid grafted procyanidine copolymer, 5 to 15 parts of dendrobium huoshanense yeast and 5 to 10 parts of N-hydroxysuccinimide. The preparation method of the probiotic composition comprises the following steps: dispersing the chlorella protein, the hyaluronic acid grafted procyanidine copolymer and the dendrobium huoshanense leavening in deionized water, then adding lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus paracasei, Grignard bacteria and N-hydroxysuccinimide, stirring for 10-20 minutes, and refrigerating for 8-12 hours at 4 DEG C to obtain the probiotic composition. The probiotic composition with the digestion promoting effect is obtained. The probiotic composition has a good digestion promoting effect, and the survival rate of probiotics in the gastrointestinal tract digestion process is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of probiotics, and in particular to a probiotic composition with digestion-promoting effect, a preparation method and application thereof. Background Art

[0002] Probiotics are a type of microorganisms that are beneficial to the human body and have multiple functions, including nutrient metabolism, lowering cholesterol, inhibiting harmful pathogenic microorganisms, maintaining the balance of intestinal flora, and regulating immune response. They mainly include bifidobacteria, lactic acid bacteria, yeast, and bacillus.

[0003] As one of the main members of the dominant flora in the human intestine, bifidobacteria can adapt to the anaerobic environment in the intestine and play a key role in regulating the host's innate and adaptive immune development and metabolic regulation. They mainly include Bifidobacterium longum, Bifidobacterium infantis and Bifidobacterium adolescentis, etc. Common lactobacilli mainly include Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus paracasei, etc.

[0004] In terms of nutritional metabolism, probiotics can promote the body's absorption of micronutrients and synthesize a variety of vitamins and amino acids necessary for human growth. They can also produce digestive enzymes such as lipase to promote the digestion and absorption of nutrients in the body. In terms of maintaining the balance of intestinal flora, probiotics mainly compete with pathogens for adhesion sites on the surface of the intestinal epithelium, produce antibacterial agents such as bacteriocins and organic acids, and compete with pathogens for limited nutrients and living space.

[0005] After probiotics are ingested by the human body, most probiotics can only survive for a relatively short time due to gastric acid, bile salts and digestive enzymes (such as pepsin, trypsin and lipase, etc.) in the digestive tract, and the growth and colonization of probiotics in the human body are inhibited. Therefore, the purpose of the present invention is to provide a probiotic composition with a digestive effect to improve the survival rate of probiotics in the gastrointestinal digestion process. Summary of the invention

[0006] Based on the technical problems existing in the background technology, the present invention proposes a probiotic composition with digestive effect, a preparation method and application, which has good digestive effect and improves the survival rate of probiotics in the gastrointestinal digestion process.

[0007] The probiotic composition with digestion-promoting effect proposed by the present invention comprises the following raw materials in parts by weight:

[0008] 30-50 parts of Lactobacillus plantarum, 10-30 parts of Lactobacillus rhamnosus, 20-40 parts of Lactobacillus paracasei, 10-30 parts of Gastonia solani, 20-30 parts of Chlorella protein, 10-20 parts of hyaluronic acid grafted proanthocyanidin copolymer, 5-15 parts of Huoshan dendrobium fermentation product, and 5-10 parts of N-hydroxysuccinimide.

[0009] Preferably, the method for preparing the hyaluronic acid grafted proanthocyanidin copolymer comprises the following steps:

[0010] S11: dispersing hyaluronic acid and proanthocyanidins in deionized water respectively;

[0011] S12: adding the activated laccase to the proanthocyanidin solution, and then mixing with the hyaluronic acid solution for reaction;

[0012] S13: After the reaction, sodium chloride and anhydrous ethanol are sequentially added to the solution to precipitate a crude product, and the crude product is dialyzed and freeze-dried to obtain a hyaluronic acid-grafted proanthocyanidin copolymer.

[0013] Preferably, the mass ratio of hyaluronic acid, proanthocyanidins and laccase is 100:70-80:5-15.

[0014] Preferably, the reaction temperature in S12 is 35-45° C. and the reaction time is 8-12 h.

[0015] Preferably, the method for preparing the fermented product of Dendrobium huoshanense comprises the following steps:

[0016] S21: Cut the Huoshan Dendrobium into segments, beat, grind and filter, and sterilize the filtrate for later use;

[0017] S22: inoculating the activated Lactobacillus plantarum into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0018] Preferably, the amount of bacteria added is 0.1-0.5% of the mass of the filtrate.

[0019] Preferably, the fermentation temperature in S22 is 30-40° C. and the fermentation time is 3-6 h.

[0020] The preparation method of the probiotic composition with the digestion-promoting effect proposed by the present invention comprises the following steps:

[0021] The chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product are dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide are added and stirred for 10-20 minutes, and refrigerated at 4°C for 8-12 hours to obtain a probiotic composition with digestion-promoting effect.

[0022] The present invention provides an application of the probiotic composition with digestion-promoting effect in the preparation of a drug for alleviating indigestion.

[0023] Beneficial technical effects of the present invention:

[0024] (1) probiotics of the present invention include Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei and Gastria, wherein Lactobacillus plantarum can inhibit and kill harmful bacteria in the gastrointestinal tract after entering the human body, and can also promote gastrointestinal motility, which helps digestion and absorption of food; Lactobacillus rhamnosus can secrete lactic acid, promote the secretion of gastric acid, help the absorption and digestion of food, which has a positive effect on improving indigestion, relieving symptoms such as abdominal distension; Lactobacillus paracasei can secrete a variety of enzyme substances, such as protease, amylase, etc., which can help the body better absorb protein and carbohydrates in food; Lactobacillus gasseri can play the effect of regulating gastrointestinal function after entering the human body, and can also promote digestion and absorption of food, and can also improve the situation of loss of appetite. The present invention uses the probiotics in combination, so that the probiotic composition has excellent digestion-promoting effect.

[0025] (2) The probiotic composition of the present invention also includes chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer, Huoshan Dendrobium fermented product and N-hydroxysuccinimide. The hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermented product (the main component is polysaccharide) can form a cross-linked network structure with chlorella protein through hydrogen bonds and other forces, thereby achieving the loading of probiotics and significantly improving the survival rate of probiotics after treatment with artificial gastric juice, thereby ensuring that the probiotic composition has a digestive effect after taking; at the same time, proanthocyanidins and Huoshan Dendrobium fermented product have certain anti-inflammatory and antioxidant effects, and can also improve the balance of intestinal flora. DETAILED DESCRIPTION

[0026] The present invention will be further explained below in conjunction with specific embodiments.

[0027] The plant lactobacillus, rhamnosus lactobacillus, paracasei lactobacillus and gassellosis of the present invention are all purchased from Yuantide Biotechnology (Tianjin) Co., Ltd.; chlorella protein, hyaluronic acid, proanthocyanidins and Huoshan dendrobium are all commercially available.

[0028] Example 1

[0029] The probiotic composition with digestion-promoting effect proposed by the present invention comprises the following raw materials in parts by weight:

[0030] 40 parts of Lactobacillus plantarum, 20 parts of Lactobacillus rhamnosus, 30 parts of Lactobacillus paracasei, 20 parts of Gastonia solani, 25 parts of Chlorella protein, 15 parts of hyaluronic acid grafted proanthocyanidin copolymer, 10 parts of Huoshan dendrobium fermentation product, and 8 parts of N-hydroxysuccinimide.

[0031] The steps of preparing the hyaluronic acid grafted proanthocyanidin copolymer are as follows:

[0032] S11: dispersing hyaluronic acid and proanthocyanidins in deionized water respectively;

[0033] S12: adding the activated laccase to the proanthocyanidin solution, and then mixing with the hyaluronic acid solution for reaction;

[0034] S13: After the reaction, sodium chloride and anhydrous ethanol are sequentially added to the solution to precipitate a crude product, and the crude product is dialyzed and freeze-dried to obtain a hyaluronic acid-grafted proanthocyanidin copolymer.

[0035] The mass ratio of hyaluronic acid, proanthocyanidin and laccase is 100:75:10; the reaction temperature is 40°C and the reaction time is 10h.

[0036] The method steps for preparing the fermented product of Dendrobium huoshanense are as follows:

[0037] S21: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0038] S22: inoculating the activated Lactobacillus plantarum into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0039] The amount of bacteria added was 0.3% of the mass of the filtrate; the fermentation temperature in S22 was 35° C. and the fermentation time was 4 h.

[0040] The preparation method of the probiotic composition with the digestion-promoting effect proposed by the present invention comprises the following steps:

[0041] The chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product are dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide are added and stirred for 15 minutes, and refrigerated at 4°C for 10 hours to obtain a probiotic composition with digestive effect.

[0042] Example 2

[0043] The probiotic composition with digestion-promoting effect proposed by the present invention comprises the following raw materials in parts by weight:

[0044] 30 parts of plant lactobacillus, 10 parts of rhamnosus lactobacillus, 20 parts of paracasei lactobacillus, 10 parts of gassellosis bacteria, 20 parts of chlorella protein, 10 parts of hyaluronic acid grafted proanthocyanidin copolymer, 5 parts of Huoshan dendrobium fermentation product, and 5 parts of N-hydroxysuccinimide.

[0045] The steps of preparing the hyaluronic acid grafted proanthocyanidin copolymer are as follows:

[0046] S11: dispersing hyaluronic acid and proanthocyanidins in deionized water respectively;

[0047] S12: adding the activated laccase to the proanthocyanidin solution, and then mixing with the hyaluronic acid solution for reaction;

[0048] S13: After the reaction, sodium chloride and anhydrous ethanol are sequentially added to the solution to precipitate a crude product, and the crude product is dialyzed and freeze-dried to obtain a hyaluronic acid-grafted proanthocyanidin copolymer.

[0049] The mass ratio of hyaluronic acid, proanthocyanidins and laccase is 100:70:5; the reaction temperature is 35°C and the reaction time is 8h.

[0050] The method steps for preparing the fermented product of Dendrobium huoshanense are as follows:

[0051] S21: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0052] S22: inoculating the activated Lactobacillus plantarum into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0053] The amount of bacteria added was 0.1% of the mass of the filtrate; the fermentation temperature in S22 was 30° C. and the fermentation time was 3 h.

[0054] The preparation method of the probiotic composition with the digestion-promoting effect proposed by the present invention comprises the following steps:

[0055] The chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product were dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide were added and stirred for 10 minutes, and refrigerated at 4°C for 8 hours to obtain a probiotic composition with digestive effect.

[0056] Example 3

[0057] The probiotic composition with digestion-promoting effect proposed by the present invention comprises the following raw materials in parts by weight:

[0058] 50 parts of plant lactobacillus, 30 parts of rhamnosus lactobacillus, 40 parts of paracasei lactobacillus, 30 parts of gassellosis bacteria, 30 parts of chlorella protein, 20 parts of hyaluronic acid grafted proanthocyanidin copolymer, 15 parts of Huoshan dendrobium fermentation product, and 10 parts of N-hydroxysuccinimide.

[0059] The steps of preparing the hyaluronic acid grafted proanthocyanidin copolymer are as follows:

[0060] S11: dispersing hyaluronic acid and proanthocyanidins in deionized water respectively;

[0061] S12: adding the activated laccase to the proanthocyanidin solution, and then mixing with the hyaluronic acid solution for reaction;

[0062] S13: After the reaction, sodium chloride and anhydrous ethanol are sequentially added to the solution to precipitate a crude product, and the crude product is dialyzed and freeze-dried to obtain a hyaluronic acid-grafted proanthocyanidin copolymer.

[0063] The mass ratio of hyaluronic acid, proanthocyanidin and laccase is 100:80:15; the reaction temperature is 45°C and the reaction time is 12h.

[0064] The method steps for preparing the fermented product of Dendrobium huoshanense are as follows:

[0065] S21: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0066] S22: inoculating the activated Lactobacillus plantarum into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0067] The amount of bacteria added was 0.5% of the mass of the filtrate; the fermentation temperature in S22 was 40° C. and the fermentation time was 6 h.

[0068] The preparation method of the probiotic composition with the digestion-promoting effect proposed by the present invention comprises the following steps:

[0069] The chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product are dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide are added and stirred for 20 minutes, and refrigerated at 4°C for 12 hours to obtain a probiotic composition with digestive effect.

[0070] Comparative Example 1

[0071] The probiotic composition with digestion-promoting effect proposed by the present invention comprises the following raw materials in parts by weight:

[0072] 40 parts of plant lactobacillus, 20 parts of rhamnosus lactobacillus, 30 parts of paracasei lactobacillus, 20 parts of gassellosis bacteria, 25 parts of chlorella protein, 25 parts of hyaluronic acid grafted proanthocyanidin copolymer, and 8 parts of N-hydroxysuccinimide.

[0073] The steps of preparing the hyaluronic acid grafted proanthocyanidin copolymer are as follows:

[0074] S11: dispersing hyaluronic acid and proanthocyanidins in deionized water respectively;

[0075] S12: adding the activated laccase to the proanthocyanidin solution, and then mixing with the hyaluronic acid solution for reaction;

[0076] S13: After the reaction, sodium chloride and anhydrous ethanol are sequentially added to the solution to precipitate a crude product, and the crude product is dialyzed and freeze-dried to obtain a hyaluronic acid-grafted proanthocyanidin copolymer.

[0077] The mass ratio of hyaluronic acid, proanthocyanidin and laccase is 100:75:10; the reaction temperature is 40°C and the reaction time is 10h.

[0078] The preparation method of the probiotic composition with the digestion-promoting effect proposed by the present invention comprises the following steps:

[0079] The chlorella protein and hyaluronic acid grafted proanthocyanidin copolymer are dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide are added and stirred for 15 minutes, and refrigerated at 4°C for 10 hours to obtain a probiotic composition with digestive effect.

[0080] Comparative Example 2

[0081] The probiotic composition with digestion-promoting effect proposed by the present invention comprises the following raw materials in parts by weight:

[0082] 40 parts of Lactobacillus plantarum, 20 parts of Lactobacillus rhamnosus, 30 parts of Lactobacillus paracasei, 20 parts of Gastonia solani, 25 parts of Chlorella protein, 25 parts of Huoshan Dendrobium fermented product, and 8 parts of N-hydroxysuccinimide.

[0083] The method steps for preparing the fermented product of Dendrobium huoshanense are as follows:

[0084] S21: 50 g of Huoshan Dendrobium officinale was cut into segments and added into 1 L of water for pulping, fine grinding and filtering. The filtrate was sterilized and concentrated to 200 mL for later use;

[0085] S22: inoculating the activated Lactobacillus plantarum into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

[0086] The amount of bacteria added was 0.3% of the mass of the filtrate; the fermentation temperature in S22 was 35° C. and the fermentation time was 4 h.

[0087] The preparation method of the probiotic composition with the digestion-promoting effect proposed by the present invention comprises the following steps:

[0088] The chlorella protein and the fermented product of Dendrobium huoshanense were dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide were added and stirred for 15 minutes, and refrigerated at 4°C for 10 hours to obtain a probiotic composition with digestive effect.

[0089] The probiotic compositions prepared in Examples 1-3 and Comparative Examples 1-2 were subjected to an in vitro digestion simulation test, and the method was as follows: 10 g of the probiotic composition sample was cut into small pieces of 1 cm × 1 cm × 1 cm, mixed with 50 mL of simulated gastric juice (pepsin 3.2 g / L, NaCl 8.5 g / L, pH 2.0), and then shaken in a water bath (100 rpm) at 37° C. for 2 h, the pH of the system was adjusted to 7.0, and then simulated intestinal juice (NaCl 8.5 g / L, bile salt 45 g / L and pancreatin 1 g / L) was added to simulate intestinal digestion at 37° C. for 2 h, and then the number of viable probiotics was determined. The test results are shown in Table 1.

[0090] Table 1 Results of in vitro digestion simulation test

[0091]

[0092] It can be seen from the test results of Example Table 1 that the probiotic composition of the present invention can still maintain a probiotic survival rate of more than 70% after simulated digestion in vitro, which is more conducive to the digestion-promoting effect of the probiotics in vivo. In addition, according to the test results of Example 1 and Comparative Examples 1 and 2, it can be seen that the carrier prepared by using chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product has a better probiotic protection effect, and the hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product have a synergistic effect in improving the survival rate of probiotics.

Claims

1. A probiotic composition having a digestive effect, characterized in that: Contains the following raw materials in parts by weight: 30-50 parts of Lactobacillus plantarum, 10-30 parts of Lactobacillus rhamnosus, 20-40 parts of Lactobacillus paracasei, 10-30 parts of Gastonia solani, 20-30 parts of Chlorella protein, 10-20 parts of hyaluronic acid grafted proanthocyanidin copolymer, 5-15 parts of Huoshan dendrobium fermentation product, and 5-10 parts of N-hydroxysuccinimide.

2. The probiotic composition with digestive effect according to claim 1, characterized in that: The method for preparing the hyaluronic acid grafted proanthocyanidin copolymer comprises the following steps: S11: dispersing hyaluronic acid and proanthocyanidins in deionized water respectively; S12: adding the activated laccase to the proanthocyanidin solution, and then mixing with the hyaluronic acid solution for reaction; S13: After the reaction, sodium chloride and anhydrous ethanol are sequentially added to the solution to precipitate a crude product, and the crude product is dialyzed and freeze-dried to obtain a hyaluronic acid-grafted proanthocyanidin copolymer.

3. The probiotic composition with digestive effect according to claim 2, characterized in that: The mass ratio of hyaluronic acid, proanthocyanidins and laccase is 100:70-80:5-15.

4. The probiotic composition with digestive effect according to claim 2, characterized in that: In S12, the reaction temperature is 35-45°C and the reaction time is 8-12h.

5. The probiotic composition with digestive effect according to claim 1, characterized in that: The method steps for preparing the Huoshan Dendrobium fermented product are as follows: S21: Cut the Huoshan Dendrobium into segments, beat, grind and filter, and sterilize the filtrate for later use; S22: inoculating the activated Lactobacillus plantarum into the sterilized filtrate of S1 for fermentation, and concentrating and drying the fermented solution to obtain the Huoshan Dendrobium fermented product.

6. The probiotic composition with digestive effect according to claim 5, characterized in that: The amount of bacteria added is 0.1-0.5% of the mass of the filtrate.

7. The probiotic composition with digestive effect according to claim 5, characterized in that: The fermentation temperature in S22 is 30-40°C and the fermentation time is 3-6h.

8. The method for preparing the probiotic composition having a digestion-promoting effect according to any one of claims 1 to 7, characterized in that: The steps are as follows: The chlorella protein, hyaluronic acid grafted proanthocyanidin copolymer and Huoshan Dendrobium fermentation product are dispersed in deionized water, and then Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Bacillus gasseri and N-hydroxysuccinimide are added and stirred for 10-20 minutes, and refrigerated at 4°C for 8-12 hours to obtain a probiotic composition with digestion-promoting effect.

9. Use of the probiotic composition with digestive effects as claimed in any one of claims 1 to 7 in the preparation of a drug for alleviating indigestion.

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