Plateau cordyceps sinensis extract anti-wrinkle composition and preparation method thereof

Abstract

The invention relates to a plateau cordyceps sinensis extract anti-wrinkle composition and a preparation method thereof, and belongs to the technical field of cosmetics. The natural whey protein, the cinnamyl aldehyde and the hydroxypropyl methyl cellulose are combined to jointly construct a compact and tough wall material structure, so that the high stability and the lasting release capability of the active ingredients of the cordyceps sinensis in use are ensured. The structure and the function of the wall material are modified by adopting a low-temperature plasma technology, so that the interaction force between the active component and the wall material is enhanced, the encapsulation efficiency is improved, the size of the microcapsule is refined, and the fineness and the uniformity of the composition are improved. Besides, cordyceps microcapsules are combined with plant essential oil, centella asiatica extract, camellia flower extract, soluble collagen and other components, a humectant and a functional additive are introduced, and the excellent permeability of glacier deuterium-depleted water is utilized, so that the prepared composition can go deep into the bottom layer of the skin, the moisture retention capacity of the skin is effectively improved, and the skin moisturizing effect is improved. The efficient combination of anti-wrinkle, soothing and moisturizing effects is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and relates to an anti-wrinkle composition of plateau cordyceps extract and a preparation method thereof. Background Art

[0002] In the field of cosmetics and skin care products, anti-wrinkle compositions are one of the important directions of research and development. There are many types of existing anti-wrinkle compositions, including chemical synthetics, plant extracts, animal-derived ingredients, etc. Plateau Cordyceps is a precious Chinese medicinal material with a variety of biologically active ingredients and pharmacological effects. Its extract is rich in nutrients such as various amino acids, polysaccharides, vitamins and minerals, and has multiple functions such as antioxidant, anti-inflammatory, and immunomodulatory. Cordyceps extracts also have broad application prospects in the field of cosmetics. However, directly mixing Cordyceps extracts in the formula of cosmetics may face problems such as poor stability, poor skin permeability and irritation. Therefore, the development of an anti-wrinkle composition with plateau Cordyceps extract as the main ingredient not only meets market demand, but also meets the current development trend of the skin care industry. Summary of the invention

[0003] The purpose of the present invention is to provide a plateau cordyceps extract anti-wrinkle composition and a preparation method thereof.

[0004] The purpose of the present invention can be achieved through the following technical solutions:

[0005] A plateau cordyceps extract anti-wrinkle composition, comprising the following raw materials by weight percentage:

[0006] 10-15% polyol moisturizer, 8-10% lactobacillus fermentation product, 5-8% triethylhexanoin, 3-5% panthenol, 3-5% acrylates / C10-30 alkyl acrylate cross-linked polymer, 2-3% carnosine, 1.5-2% plateau cordyceps microcapsules, 1.1-1.3% PEG-40 hydrogenated castor oil, 1-1.2% disodium EDTA, 0.8-1% plant essence oil, 0.7-0.9% trehalose, 0.4-0.6% soluble collagen, 0.3-0.5% Centella asiatica extract, 0.3-0.5% squalane, 0.2-0.4% camellia extract, 0.3-0.5% preservatives, and the balance is deuterated water.

[0007] Furthermore, the polyol moisturizer is glycerol, 1,3-propylene glycol, 1,2-hexanediol, and butylene glycol in a mass ratio of 1:2:2:2.

[0008] Furthermore, the preservative is p-hydroxyacetophenone.

[0009] Furthermore, the plant essential oil is a mixture of meadowfoam seed oil and oat kernel oil in a mass ratio of 1:1.

[0010] Furthermore, the preparation method of the plateau cordyceps microcapsules is as follows:

[0011] S5-1: 3-5 parts by weight of whey protein and 1-2 parts by weight of hydroxypropyl methylcellulose were mixed, 30-50 parts by weight of deionized water were added, ultrasonic dispersion was performed for 30 minutes, 0.5 parts by weight of cinnamaldehyde was added under stirring at 50° C., ultrasonic dispersion was performed for 30 minutes, and then 3-4 parts by weight of cordyceps sinensis extract were added, stirring was continued for 1 hour, and then 10 parts by weight of emulsifier were added, and stirring was performed at 60° C. for 30 minutes to obtain emulsion A;

[0012] S5-2: Soybean oil and emulsion A are mixed under stirring at 60°C, and placed in a quartz reactor for low-temperature plasma treatment. The parameters of the low-temperature plasma treatment are set to an input current of 1 to 1.2 A, an input power of 50 to 70 W, and a treatment time of 20 to 30 s. The mixture is rapidly sheared for 3 to 5 minutes using a high-speed shearing machine, and freeze-dried at -40°C for 12 hours to obtain the plateau Cordyceps microcapsules.

[0013] Furthermore, the emulsifier in S5-1 is a mixture of glyceryl monostearate, Tween 20 and deionized water in a mass ratio of 1:1:18.

[0014] Furthermore, in the S5-2, the soybean oil and the emulsion A are mixed in a mass ratio of (2-3):1.

[0015] Furthermore, the rotation speed of the high-speed shearing machine in S5-2 is 10000-12000 r / min.

[0016] A method for preparing a plateau cordyceps extract anti-wrinkle composition, the specific steps of the preparation method are as follows:

[0017] S9-1: According to the formula, a polyol moisturizer, a lactobacillus fermentation product, triethylhexanoin, panthenol and deuterium-depleted water were mixed, stirred at a speed of 200 to 300 r / min for 15 to 25 min, and homogenized for 30 min to obtain phase liquid A;

[0018] S9-2: Mix plateau cordyceps microcapsules, carnosine, plant extract oil, trehalose, soluble collagen, Centella asiatica extract, squalane, camellia extract and PEG-40 hydrogenated castor oil, stir for 10 minutes, and then add to phase liquid A and homogenize for 1 hour to obtain phase liquid B;

[0019] S9-3: adding preservatives, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer and disodium EDTA in sequence, and homogenizing for 30 minutes to obtain the anti-wrinkle composition.

[0020] Furthermore, the stirring speed in S9-2 is 100 r / min.

[0021] The active ingredients in Cordyceps sinensis extract, such as cordycepin, polysaccharides, amino acids and trace elements, can promote the proliferation of skin cells and the synthesis of collagen, and improve the elasticity and firmness of the skin. In addition, Cordyceps sinensis has antioxidant, anti-inflammatory and skin repair effects, which can effectively delay the aging process of the skin. Microencapsulation technology can delay the release of Cordyceps sinensis extract, making it work more lastingly, and improving the durability and effect of cosmetics.

[0022] Natural whey protein has good biocompatibility and degradability, and is suitable for use as a carrier material for encapsulating Cordyceps sinensis extract. Whey protein can not only gently encapsulate active ingredients, but also form a protective film on the skin to enhance the skin's moisturizing and barrier functions. However, when whey protein is used alone as a wall material, the mechanical strength of the microcapsule may be insufficient and cannot completely shield the impact of environmental factors.

[0023] Cinnamaldehyde comes from natural plants and has antibacterial and antioxidant activities. It can also give the composition a long-lasting natural aroma. During the preparation of microcapsules, the aldehyde group in cinnamaldehyde reacts with the amino group in whey protein to form a Schiff base, thereby changing the structure and properties of whey protein, enhancing the interfacial activity of whey protein, enhancing the stability and encapsulation rate of microcapsules, and improving the quality of the product.

[0024] Hydroxypropyl methylcellulose can significantly increase the viscosity and stability of the wall material, and work synergistically with whey protein to build a denser and tougher wall material structure. This structure provides more solid protection for the active ingredients in the Cordyceps sinensis extract, ensuring its stability and durability in the composition and extending the storage time.

[0025] In order to further optimize the performance of microcapsules, low-temperature plasma was used to modify the structure and functional properties of whey protein and hydroxypropyl methylcellulose, enhance the binding force between cordyceps extract and wall material, thereby improving the encapsulation rate of wall material and core material, and playing a positive role in the processing and storage stability of microcapsules. In addition, low-temperature plasma treatment can also promote the formation of microcapsules with smaller size and more uniform distribution.

[0026] Soluble collagen has a smaller molecular weight and can penetrate from the skin surface into the deeper dermis to provide nutrition and support to the skin; soluble collagen has a good moisturizing effect and can absorb and retain a large amount of water molecules, thereby helping the skin stay hydrated and smooth; soluble collagen can stimulate and promote the synthesis of collagen, help repair damaged collagen, and promote the production of new collagen, thereby improving the skin's elasticity and firmness.

[0027] Lactobacillus fermentation products contain lactobacillus and other polypeptide compounds with antibacterial effects. They are natural antibacterial agents and adhere to epidermal cells to form a protective barrier for the skin. They also contain amino acids, polysaccharides, B vitamins and other ingredients, which can enhance stratum corneum metabolism, remove cellular free radicals, and inhibit lipid peroxidation. In addition, Lactobacillus fermentation products are rich in lactic acid and sodium lactate, which are the main water-soluble acids in the human body's natural moisturizing factors. They have strong hygroscopicity and prevent skin moisture loss. Lactic acid and sodium lactate form a buffer solution, which has good affinity to the skin and can regulate the pH value of the skin.

[0028] Oat kernel oil is rich in linoleic acid, an essential fatty acid for the human body. It also contains natural neurotropic amine, which acts on human skin and can combine with moisture in the stratum corneum to form a network structure to firmly lock in moisture in the skin. Limnanthes alba seed oil contains more than 98% long-chain fatty acids with antioxidant effects, which can form a water-locking film on the skin surface; Limnanthes alba seed oil also has good antioxidant properties, and can also stretch skin texture, deeply repair skin damage, and enhance the overall contour of the skin; in addition, Limnanthes alba seed oil can also improve the stability of oat kernel oil.

[0029] Squalane has good skin affinity and is highly compatible with the sebum secreted by the skin, forming a breathable protective film on the surface of the skin, exerting a moisturizing and emollient effect; Squalane has good permeability and can enter the skin, replenish cell lipids, and nourish the skin.

[0030] Centella asiatica extract is rich in antioxidant ingredients, which can inhibit the activity of free radicals, prevent oxidative stress caused by free radicals, and improve skin blood circulation; Centella asiatica extract contains asiaticoside, which can reduce the production of inflammatory mediators, inhibit the activity of enzymes in inflammatory reactions, reduce inflammatory reactions, and soothe the skin. Camellia extract can effectively inhibit the activity of skin inflammatory cells, relieve skin allergy symptoms, and have antibacterial and anti-inflammatory effects; in addition, camellia extract can effectively shrink pores and inhibit skin oil secretion, and is an excellent natural active ingredient.

[0031] Deuterium-depleted water can more easily penetrate into the deep layers of the skin, combine with the water in the skin cells, and form a stable hydration layer, thereby effectively locking in moisture and reducing water loss. Deuterium-depleted water can activate human cells and functions and promote the metabolism of skin cells.

[0032] Beneficial effects of the present invention:

[0033] (1) The active ingredients in Cordyceps sinensis extract are combined with microencapsulation technology to significantly promote skin cell regeneration and collagen synthesis, enhance skin elasticity and firmness, and effectively delay skin aging. Natural whey protein is used as a carrier, combined with the antibacterial and antioxidant properties of cinnamaldehyde, and the stability and encapsulation rate of microcapsules are enhanced through Schiff base reaction. Hydroxypropyl methylcellulose is added to further construct a dense wall material structure to protect the active ingredients. Innovative low-temperature plasma technology is used to modify the wall material structure, enhance binding force, improve processing and storage stability, and promote the small size and uniform distribution of microcapsules.

[0034] (2) The cordyceps microcapsules are further combined with plant essential oils, Centella asiatica extract, Camellia extract, soluble collagen and other ingredients, which not only fully utilizes the sustained release characteristics of the active ingredients in the cordyceps microcapsules, but also integrates the nourishing and repairing functions of various plant essences; at the same time, through the combination of moisturizers and other functional additives, and the introduction of glacial deuterium-rich water rich in mineral components, the excellent penetration performance allows the entire composition to penetrate deep into the skin, effectively improve the skin's moisture retention capacity, and promote the long-term anti-wrinkle effect. DETAILED DESCRIPTION

[0035] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0036] In the embodiments and comparative examples:

[0037] The polyol humectant is glycerol, 1,3-propylene glycol, 1,2-hexanediol, and butylene glycol in a mass ratio of 1:2:2:2;

[0038] Lactobacillus fermentation products: purchased from Anhui Shunli Biological Co., Ltd.;

[0039] Glyceryl tri(ethylhexanoate): purchased from Shanghai MacLean Biochemical Technology Co., Ltd.;

[0040] Panthenol: purchased from Suzhou Sanyi Polymer Chemical Technology Co., Ltd.;

[0041] Acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer: purchased from Hubei Shiteng Chemical Technology Co., Ltd.;

[0042] Carnosine: purchased from Hubei Hongpeptide Biotechnology Co., Ltd.;

[0043] Cordyceps sinensis extract was purchased from Hunan Langlin Biological Resources Co., Ltd.;

[0044] PEG-40 hydrogenated castor oil: purchased from Chengdu Huaxia Chemical Reagent Co., Ltd.;

[0045] Disodium EDTA: purchased from Tianjin Guangfu Technology Development Co., Ltd.;

[0046] Trehalose: purchased from Xi'an Jinxiang Pharmaceutical Excipients Co., Ltd.

[0047] Limnanthes alba seed oil: purchased from Sichuan Weikeqi Biotechnology Co., Ltd.;

[0048] Oat kernel oil: purchased from Yikang Natural Spice Oil Refinery, Jishui County;

[0049] Soluble collagen: purchased from Baijiawei (Shenzhen) Biotechnology Co., Ltd.;

[0050] Centella asiatica extract: purchased from Guilin Yitiancheng Biotechnology Co., Ltd.;

[0051] Squalane: purchased from Hebei Zhentian Food Additive Co., Ltd.;

[0052] Camellia extract: purchased from Shaanxi New Horizon Biotechnology Co., Ltd.;

[0053] Whey protein: purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0054] Hydroxypropyl methylcellulose: purchased from Zhejiang Haishen New Materials Co., Ltd.;

[0055] Cinnamaldehyde: purchased from Wuhan Nengmaike Industrial Co., Ltd.;

[0056] Soybean oil: purchased from Guangzhou Baiyunshan Hanfang Modern Pharmaceutical Co., Ltd.;

[0057] Glyceryl monostearate: purchased from Shanghai Bangcheng Chemical Co., Ltd.;

[0058] Tween 20: purchased from Guangdong Runhua Chemical Co., Ltd.;

[0059] p-Hydroxyacetophenone: purchased from Jiangsu Xinhan New Materials Co., Ltd.

[0060] The deuterium content of deuterium-depleted water is <150ppm.

[0061] Example 1

[0062] A plateau cordyceps extract anti-wrinkle composition, formula 1 comprises the following raw materials in terms of weight percentage:

[0063] 12% polyol moisturizer, 9% lactobacillus fermentation product, 7% triethylhexanoin, 4% panthenol, 4% acrylates / C10-30 alkyl acrylate crosspolymer, 2.5% carnosine, 1.7% plateau cordyceps microcapsules, 1.2% PEG-40 hydrogenated castor oil, 1.1% disodium EDTA, 0.4% meadowfoam seed oil, 0.4% oat kernel oil, 0.8% trehalose, 0.5% soluble collagen, 0.4% Centella asiatica extract, 0.4% squalane, 0.3% camellia extract, 0.4% p-hydroxyacetophenone, and the balance is deuterated water.

[0064] The preparation method of the plateau cordyceps microcapsules is as follows:

[0065] S5-1: 4 parts by weight of whey protein and 1.5 parts by weight of hydroxypropyl methylcellulose were mixed, 40 parts by weight of deionized water were added, ultrasonic dispersion was performed for 30 minutes, 0.5 parts by weight of cinnamaldehyde was added under stirring at 50°C, ultrasonic dispersion was performed for 30 minutes, and then 3.5 parts by weight of Cordyceps sinensis extract was added, stirring was continued for 1 hour, and then 10 parts by weight of emulsifier was added, wherein the emulsifier was glyceryl monostearate, Tween 20 and deionized water mixed in a mass ratio of 1:1:18, and stirred at 60°C for 30 minutes to obtain emulsion A;

[0066] S5-2: Soybean oil and emulsion A were mixed at a mass ratio of 2.5:1 under stirring at 60°C, and placed in a quartz reactor for low-temperature plasma treatment. The parameters of the low-temperature plasma treatment were set to input current of 1.1A, input power of 60W, and treatment time of 25s. The mixture was rapidly sheared at a speed of 11000r / min for 4min using a high-speed shearing machine, and freeze-dried at -40°C for 12h to obtain the plateau Cordyceps microcapsules.

[0067] A method for preparing a plateau cordyceps extract anti-wrinkle composition, the specific steps of the preparation method are as follows:

[0068] S9-1: According to formula 1, a polyol moisturizer, a lactobacillus fermentation product, triethylhexanoin, panthenol and deuterium-depleted water were mixed, stirred at a speed of 250 r / min for 20 min, and homogenized for 30 min to obtain phase liquid A;

[0069] S9-2: Mix Cordyceps microcapsules, carnosine, meadowfoam seed oil, oat kernel oil, trehalose, soluble collagen, Centella asiatica extract, squalane, camellia extract and PEG-40 hydrogenated castor oil, stir at a speed of 100 r / min for 10 min, and then add to phase liquid A for homogenization for 1 h to obtain phase liquid B;

[0070] S9-3: adding p-hydroxyacetophenone, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer and disodium EDTA in sequence, and homogenizing for 30 minutes to obtain the anti-wrinkle composition.

[0071] Example 2

[0072] A plateau cordyceps extract anti-wrinkle composition, formula 2 comprises the following raw materials in terms of weight percentage:

[0073] 10% polyol moisturizer, 8% lactobacillus fermentation product, 5% triethylhexanoin, 3% panthenol, 3% acrylates / C10-30 alkyl acrylate crosspolymer, 2% carnosine, 1.5% plateau cordyceps microcapsules, 1.1% PEG-40 hydrogenated castor oil, 1% disodium EDTA, 0.4% meadowfoam seed oil, 0.4% oat kernel oil, 0.7% trehalose, 0.4% soluble collagen, 0.3% Centella asiatica extract, 0.3% squalane, 0.2% camellia extract, 0.3% p-hydroxyacetophenone, and the balance is deuterated water.

[0074] The preparation method of the plateau cordyceps microcapsules is as follows:

[0075] S5-1: 3 parts by weight of whey protein and 1 part by weight of hydroxypropyl methylcellulose were mixed, 30 parts by weight of deionized water were added, ultrasonic dispersion was performed for 30 minutes, 0.5 parts by weight of cinnamaldehyde was added under stirring at 50°C, ultrasonic dispersion was performed for 30 minutes, and then 3 parts by weight of Cordyceps sinensis extract was added, stirring was continued for 1 hour, and then 10 parts by weight of emulsifier was added, wherein the emulsifier was glyceryl monostearate, Tween 20 and deionized water mixed in a mass ratio of 1:1:18, and stirred at 60°C for 30 minutes to obtain emulsion A;

[0076] S5-2: Soybean oil and emulsion A were mixed in a mass ratio of 2:1 under stirring at 60°C, and placed in a quartz reactor for low-temperature plasma treatment. The parameters of the low-temperature plasma treatment were set to input current of 1A, input power of 50W, and treatment time of 20s. The mixture was rapidly sheared at a speed of 10,000 r / min for 3 minutes using a high-speed shearing machine, and freeze-dried at -40°C for 12 hours to obtain the plateau Cordyceps microcapsules.

[0077] A method for preparing a plateau cordyceps extract anti-wrinkle composition, the specific steps of the preparation method are as follows:

[0078] S9-1: According to formula 2, a polyol moisturizer, a lactobacillus fermentation product, triethylhexanoin, panthenol and deuterium-depleted water were mixed, stirred at a speed of 200 r / min for 15 min, and homogenized for 30 min to obtain phase liquid A;

[0079] S9-2: Mix Cordyceps microcapsules, carnosine, meadowfoam seed oil, oat kernel oil, trehalose, soluble collagen, Centella asiatica extract, squalane, camellia extract and PEG-40 hydrogenated castor oil, stir at a speed of 100 r / min for 10 min, and then add to phase liquid A for homogenization for 1 h to obtain phase liquid B;

[0080] S9-3: adding p-hydroxyacetophenone, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer and disodium EDTA in sequence, and homogenizing for 30 minutes to obtain the anti-wrinkle composition.

[0081] Example 3

[0082] A plateau cordyceps extract anti-wrinkle composition, formula 3 comprises the following raw materials by weight percentage:

[0083] 15% polyol moisturizer, 10% lactobacillus fermentation product, 8% triethylhexanoin, 5% panthenol, 5% acrylates / C10-30 alkyl acrylate crosspolymer, 3% carnosine, 2% plateau cordyceps microcapsule, 1.3% PEG-40 hydrogenated castor oil, 1.2% disodium EDTA, 0.5% meadowfoam seed oil, 0.5% oat kernel oil, 0.9% trehalose, 0.6% soluble collagen, 0.5% Centella asiatica extract, 0.5% squalane, 0.4% camellia extract, 0.5% p-hydroxyacetophenone, the balance is deuterium-depleted water.

[0084] The preparation method of the plateau cordyceps microcapsules is as follows:

[0085] S5-1: 5 parts by weight of whey protein and 2 parts by weight of hydroxypropyl methylcellulose were mixed, 50 parts by weight of deionized water were added, ultrasonic dispersion was performed for 30 minutes, 0.5 parts by weight of cinnamaldehyde was added under stirring at 50°C, ultrasonic dispersion was performed for 30 minutes, 4 parts by weight of cordyceps sinensis extract was added, stirring was continued for 1 hour, and then 10 parts by weight of emulsifier was added, wherein the emulsifier was glyceryl monostearate, Tween 20 and deionized water mixed in a mass ratio of 1:1:18, and stirred at 60°C for 30 minutes to obtain emulsion A;

[0086] S5-2: Soybean oil and emulsion A were mixed in a mass ratio of 3:1 under stirring at 60°C, and placed in a quartz reactor for low-temperature plasma treatment. The parameters of the low-temperature plasma treatment were set to input current of 1.2A, input power of 70W, and treatment time of 30s. The mixture was rapidly sheared at a speed of 12000r / min for 5min using a high-speed shearing machine, and freeze-dried at -40°C for 12h to obtain the plateau Cordyceps microcapsules.

[0087] A method for preparing a plateau cordyceps extract anti-wrinkle composition, the specific steps of the preparation method are as follows:

[0088] S9-1: According to formula 3, a polyol moisturizer, a lactobacillus fermentation product, triethylhexanoin, panthenol and deuterium-depleted water were mixed, stirred at a speed of 300 r / min for 25 min, and homogenized for 30 min to obtain phase liquid A;

[0089] S9-2: Mix Cordyceps microcapsules, carnosine, meadowfoam seed oil, oat kernel oil, trehalose, soluble collagen, Centella asiatica extract, squalane, camellia extract and PEG-40 hydrogenated castor oil, stir at a speed of 100 r / min for 10 min, and then add to phase liquid A for homogenization for 1 h to obtain phase liquid B;

[0090] S9-3: adding p-hydroxyacetophenone, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer and disodium EDTA in sequence, and homogenizing for 30 minutes to obtain the anti-wrinkle composition.

[0091] Comparative Example 1

[0092] During the preparation of the plateau cordyceps microcapsules, low-temperature plasma treatment is not performed, and the remaining steps are consistent with those in Example 1.

[0093] Comparative Example 2

[0094] During the preparation of the plateau Cordyceps microcapsules, hydroxypropyl methylcellulose was not added, and the remaining steps were consistent with those in Example 1.

[0095] Comparative Example 3

[0096] During the preparation of plateau cordyceps microcapsules, cinnamaldehyde was not added, and the remaining steps were consistent with those in Example 1.

[0097] Comparative Example 4

[0098] The plateau Cordyceps extract was directly added without being processed by forming microcapsules, and the other operations were consistent with those in Example 1.

[0099] 1. Moisturizing efficacy test

[0100] According to the laboratory method (XYJC-SOP-CE-022 Cosmetic Moisturizing Efficacy Test Method - Weighing Method), the moisturizing rate of Example 1 was tested. Tested substances: The sample group is the original sample of Example 1, and the positive control group is 5% glycerol. Test steps: Weigh a certain amount of sample and positive control and place them on the test glass plate, and then place them in a constant temperature and humidity chamber. The temperature in the constant temperature and humidity chamber is 20°C and the relative humidity is 50% ± 5%. The moisturizing rate of the sample and the positive control is calculated by measuring the ratio of the sample amount to the initial sample amount after the sample and the positive control are placed for 2h, 4h, and 8h. The formula is as follows:

[0101] Moisture retention rate = (Mt -M0) / M×100%

[0102] Where: M t is the mass of the sample (positive control) and the plate after being placed for 2h, 4h, and 8h; M0 is the mass of the empty plate; M is the mass of the weighed sample (positive control).

[0103] The results are shown in Table 1:

[0104] Table 1

[0105] Group 2h 4h 8h Example 1 86.11％±1.240％ 72.56％±1.789％ 51.90％±2.577％ Positive Control 44.51％±1.085％ 16.24％±1.510％ 8.45％±0.492％

[0106] According to the test results in Table 1, the moisturizing rates of the samples at 2h, 4h and 8h are 86.11%, 72.56% and 51.90% respectively. It can be seen that the moisturizing rates of the test samples at 2h, 4h and 8h are higher than those of the positive control, indicating that the anti-wrinkle composition prepared in Example 1 has moisturizing effect.

[0107] 2. Soothing effect test

[0108] According to the laboratory method (XYJC-SOP-CE-021 hyaluronidase inhibition rate method), the soothing effect of Example 1 was tested. Test method: The sample group is a 10% aqueous solution of the sample of Example 1, the negative control group is pure water, and the positive control group is a 1% dipotassium glycyrrhizinate solution; four groups ABCD are set up respectively, and 3 parallels are set up in each group. Calcium chloride, buffer solution, sample solution, sodium hydroxide, acetylacetone and other solutions are added to the test tube in succession, mixed, and then placed in boiling water, ice water, and room temperature in succession. After the reaction, P-DAB color developer is added to the four tubes. After the solution inside reacts completely, the absorbance value of each group is measured at a wavelength of 530nm, and then the hyaluronidase inhibition rate is calculated. The formula is as follows:

[0109] Hyaluronidase inhibition rate = [(CD)-(AB)] / (CD)×100%

[0110] Wherein: A is the absorbance of the reaction solution containing sample and enzyme, B is the absorbance of the reaction solution containing sample and no enzyme, C is the absorbance of the reaction solution containing enzyme but no sample, and D is the absorbance of the reaction solution excluding sample and enzyme.

[0111] The results are shown in Table 2:

[0112] Table 2

[0113]

[0114]

[0115] According to the test results in Table 2, when the experimental system is guaranteed to be effective (the hyaluronidase inhibition rate of the positive control is greater than 50%), when the sample concentration is 10%, the hyaluronidase inhibition rate is 48.848%, which is significantly different from that of the negative control (P<0.05), indicating that the anti-wrinkle composition prepared in Example 1 has a soothing effect.

[0116] 3. Anti-wrinkle and firming efficacy test

[0117] According to the laboratory method (XYJC-SOP-CE-019 elastase inhibition rate method), the anti-wrinkle and firming effects of the embodiments and comparative examples were tested. Test method: The sample groups were 10% sample aqueous solutions of embodiments 1 to 3 and comparative examples 1 to 3; the negative control group was pure water; the positive control group was 1% EGCG solution; 3 parallels were set up for each group, and the buffer solution, sample solution, enzyme solution and substrate were added to the 96-well plate in sequence, and the 96-well plate was placed in a low-temperature shaking water tank at a certain temperature, and the shaking mode was turned on. After the solution inside reacted completely, the absorbance value of each group was measured at a wavelength of 410nm, and then the elastase inhibition rate was calculated. The formula is as follows:

[0118] Elastase inhibition rate = [(CD)-(AB)] / (CD)×100%

[0119] Wherein: A is the absorbance of the reaction solution containing sample and enzyme; B is the absorbance of the reaction solution containing sample and no enzyme; C is the absorbance of the reaction solution containing enzyme but no sample; D is the absorbance of the reaction solution excluding sample and enzyme.

[0120] The results are shown in Table 3:

[0121] Table 3

[0122]

[0123]

[0124] According to the test results in Table 3, under the premise of ensuring the effectiveness of the test reaction system (positive control elastase inhibition rate>50%), the elastase inhibition rate of the test sample is higher than that of the negative control, and there is a significant difference between the two (P<0.05), indicating that the test sample has anti-wrinkle and firming effects. It can be seen from the data of the embodiments and comparative examples that the preparation of plateau Cordyceps extract into microcapsules effectively improves the anti-wrinkle and firming effects of the sample.

[0125] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A plateau Cordyceps extract anti-wrinkle composition, characterized in that: In terms of weight percentage, the following raw materials are included: 10-15% polyol moisturizer, 8-10% lactobacillus fermentation product, 5-8% triethylhexanoin, 3-5% panthenol, 3-5% acrylates / C10-30 alkyl acrylate cross-linked polymer, 2-3% carnosine, 1.5-2% plateau cordyceps microcapsules, 1.1-1.3% PEG-40 hydrogenated castor oil, 1-1.2% disodium EDTA, 0.8-1% plant essence oil, 0.7-0.9% trehalose, 0.4-0.6% soluble collagen, 0.3-0.5% Centella asiatica extract, 0.3-0.5% squalane, 0.2-0.4% camellia extract, 0.3-0.5% preservatives, and the balance is deuterated water.

2. The anti-wrinkle composition of plateau Cordyceps extract according to claim 1, characterized in that: The polyol moisturizer is glycerol, 1,3-propylene glycol, 1,2-hexanediol and butylene glycol in a mass ratio of 1:2:2:

2.

3. The anti-wrinkle composition of plateau Cordyceps extract according to claim 1, characterized in that: The preservative is p-hydroxyacetophenone.

4. The anti-wrinkle composition of plateau Cordyceps extract according to claim 1, characterized in that: The plant essential oil is a mixture of meadowfoam seed oil and oat kernel oil in a mass ratio of 1:

1.

5. The anti-wrinkle composition of plateau Cordyceps extract according to claim 1, characterized in that: The preparation method of the plateau cordyceps microcapsules is as follows: S5-1: 3-5 parts by weight of whey protein and 1-2 parts by weight of hydroxypropyl methylcellulose were mixed, 30-50 parts by weight of deionized water were added, ultrasonic dispersion was performed for 30 minutes, 0.5 parts by weight of cinnamaldehyde was added under stirring at 50° C., ultrasonic dispersion was performed for 30 minutes, and then 3-4 parts by weight of cordyceps sinensis extract were added, stirring was continued for 1 hour, and then 10 parts by weight of emulsifier were added, and stirring was performed at 60° C. for 30 minutes to obtain emulsion A; S5-2: Soybean oil and emulsion A are mixed under stirring at 60°C, and placed in a quartz reactor for low-temperature plasma treatment. The parameters of the low-temperature plasma treatment are set to an input current of 1 to 1.2 A, an input power of 50 to 70 W, and a treatment time of 20 to 30 s. The mixture is rapidly sheared for 3 to 5 minutes using a high-speed shearing machine, and freeze-dried at -40°C for 12 hours to obtain the plateau Cordyceps microcapsules.

6. The anti-wrinkle composition of plateau Cordyceps extract according to claim 5, characterized in that: The emulsifier in S5-1 is glyceryl monostearate, Tween 20 and deionized water mixed in a mass ratio of 1:1:

18.

7. The anti-wrinkle composition of plateau Cordyceps extract according to claim 5, characterized in that: In the S5-2, soybean oil and emulsion A are mixed in a mass ratio of (2-3):

1.

8. The anti-wrinkle composition of plateau Cordyceps extract according to claim 5, characterized in that: The rotation speed of the high-speed shearing machine in S5-2 is 10000-12000 r / min.

9. A method for preparing the anti-wrinkle composition of plateau Cordyceps extract as claimed in claims 1 to 8, characterized in that: The specific steps of the preparation method are as follows: S9-1: According to the formula, a polyol moisturizer, a lactobacillus fermentation product, triethylhexanoin, panthenol and deuterium-depleted water were mixed, stirred at a speed of 200 to 300 r / min for 15 to 25 min, and homogenized for 30 min to obtain phase liquid A; S9-2: Mix plateau cordyceps microcapsules, carnosine, plant extract oil, trehalose, soluble collagen, Centella asiatica extract, squalane, camellia extract and PEG-40 hydrogenated castor oil, stir for 10 minutes, and then add to phase liquid A and homogenize for 1 hour to obtain phase liquid B; S9-3: adding preservatives, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer and disodium EDTA in sequence, and homogenizing for 30 minutes to obtain the anti-wrinkle composition.

10. The method for preparing a plateau Cordyceps extract anti-wrinkle composition according to claim 9, characterized in that: The stirring speed in S9-2 is 100 r / min.

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