A strain of Pseudomonas and its application in deodorizing kitchen waste

Abstract

The invention discloses a strain of Pseudomonas and its application in deodorizing kitchen waste, belonging to the field of environmental microbial technology. The strain is classified and named as Pseudomonas sp., and was deposited in the General Microbiology Center of China Microbiological Culture Collection Committee on April 15, 2024, with a deposit number of CGMCC No. 30345, and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code: 100101. After application, the strain can efficiently remove hydrogen sulfide, ammonia, VOCs and other components in malodorous gases, providing a new, efficient and environmentally friendly strain resource for solving the problem of air pollution caused by garbage and waste disposal.

Description

Technical Field

[0001] The invention relates to a strain and application thereof in deodorization, in particular to a strain of Pseudomonas and application thereof in deodorization of kitchen waste, belonging to the technical field of environmental microorganisms. Background Art

[0002] The malodorous components such as ammonia, hydrogen sulfide, VOCs, etc. produced during the long-term disorderly stacking and treatment of food waste have become one of the main factors affecting people's normal life and physical and mental health, and urgently need to be dealt with in a timely manner.

[0003] At present, physical adsorption, chemical oxidation, catalytic oxidation and other methods can remove malodorous gases. Although these methods may have a good deodorizing effect in a short period of time, most of them require a large amount of energy and may produce harmful or difficult-to-handle byproducts. Biological deodorization is also a major deodorizing method currently used. The odor molecules in the exhaust gas dissolve and diffuse into the surface of the microbial membrane. The microorganisms convert the malodorous substances into harmless carbon dioxide, water, sulfates, nitrates and other inorganic substances through biological metabolism (often oxidation reactions).

[0004] Compared with physical and chemical methods, biological deodorization has advantages in terms of environmental protection and sustainability: the biological reaction process can be carried out continuously and is easy to maintain; it does not require a large amount of additional energy input, and saves energy and reduces emissions.

[0005] At present, patent CN116855389A discloses a Candida plicata G5 and its application in degrading the odor of food waste, specifically, the biological deodorant made by the bacteria is used for deodorizing food waste; patent CN104312938B discloses a strain of Pseudomonas putida and the application of the agent in degrading volatile organic compounds; patent CN116731883A discloses a composite microbial deodorant and its deodorization application, the strains include microbial strains Trichosporon africanus, Ochrobacterium intermedia, Candida ethanolica, Bacillus tropicalis and Staphylococcus worderi, and the ideal effect is obtained; however, the existing patents still have the defects of weak odor removal ability and low removal rate, and in the face of people's demand for deodorization effect under different deodorization scenes, temperatures, and pH, the development and combination of different deodorization strains are still needed to achieve the ideal effect. In order to deal with the odor problem generated in the process of food waste treatment and reduce pollution, finding more universal and efficient deodorization strains existing in nature is a technical problem that technicians in this field need to solve. Summary of the invention

[0006] The technical problem to be solved by the present invention is to propose a strain of Pseudomonas and its application in deodorizing food waste in view of the shortcomings of the above-mentioned prior art. The bacteria can efficiently remove components such as hydrogen sulfide, ammonia and VOCs in malodorous gases, providing a new, efficient and environmentally friendly strain resource for solving the air pollution problem caused by the disposal of garbage and waste.

[0007] The technical solution of the present invention to solve the above technical problems is:

[0008] A strain of Pseudomonas, classified and named Pseudomonas sp., was deposited in the General Microbiology Center of China Microorganism Culture Collection on April 15, 2024, with the deposit number: CGMCC No.30345. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code: 100101.

[0009] The technical solution further defined in the present invention is:

[0010] Furthermore, in the aforementioned Pseudomonas, under laboratory conditions, the strain rapidly grew to a maximum effective viable count of 10 within 12 hours at 30°C. 10 CFU / mL; and grow normally under the conditions of 20-50℃, pH4-11, and sodium hypochlorite 0-5mg / L.

[0011] In the aforementioned Pseudomonas, the 16SrRNA gene sequence of the strain is shown as SEQ ID NO.1.

[0012] The present invention also designs a bacterial agent containing Pseudomonas with a preservation number of CGMCC No. 30345. The Pseudomonas is cultured and fermented to form a seed liquid, which is then amplified and fermented to form a biological bacterial agent.

[0013] The present invention also provides an application of Pseudomonas in deodorizing restaurant and kitchen waste.

[0014] The technical solution further defined in the present invention is:

[0015] Furthermore, in the application of the aforementioned Pseudomonas in the deodorization of food waste, under a laboratory environment, the strain or the bacterial agent containing the Pseudomonas can achieve a deammonification rate of 23.6±0.9% in the 6th hour, and an ammonia removal effect of 96.0±0.03% in the 24th hour; a desulfurization rate of 17.2±2.3% can be achieved in the 12th hour, and a desulfurization effect of 90.1±3.6% can be achieved in the 48th hour.

[0016] The application of the aforementioned Pseudomonas in deodorizing food waste specifically comprises the following steps:

[0017] (1) Inoculating Pseudomonas or a bacterial agent containing the Pseudomonas into a bioreactor, and allowing the strain to form a biofilm on the filler inside the bioreactor;

[0018] (2) After the membrane is completed, the odor generated during the treatment of food waste is introduced through a fan, and the odor is decomposed into CO under the action of the strain. 2 , water and other harmless substances, and then discharged.

[0019] In the aforementioned application of Pseudomonas in the deodorization of food waste, the bioreactor is a biofilter column, and the average VOCs removal rate reaches 95.9%.

[0020] In the aforementioned application of Pseudomonas in the deodorization of food waste, the bioreactor is a biofilter and the average removal rate of VOCs is 92.0%.

[0021] The beneficial effects of the present invention are:

[0022] The present invention separates and screens the dominant Pseudomonas bacteria that can efficiently remove hydrogen sulfide, ammonia and other VOCs from the food waste samples. The strain has a high removal rate and strong odor removal ability. According to the test, the Pseudomonas bacteria or the bacterial agent containing the Pseudomonas bacteria can quickly grow to the maximum effective viable count within 12 hours at 30°C, which can reach 10 10 CFU / mL, and can grow normally under the conditions of pH 4-11 and sodium hypochlorite 0-5mg / L. When the bacteria or the bacterial agent containing the bacteria is cultured in the laboratory, the reaction is carried out in a conical flask, and the changes in ammonia and hydrogen sulfide before and after are measured. The removal rate of ammonia can reach more than 95%; the removal rate of hydrogen sulfide can reach more than 86%. The bacteria has high vitality and strong adaptability, and can remove hydrogen sulfide and ammonia under a wide range of pH conditions; after being added to the biofilter column or biofilter, it also has a stable removal effect on volatile organic compounds (VOCs). The odorous gas is fully in contact with the microorganisms coated in the filler at a suitable residence time and air velocity, and is decomposed by the microorganisms into S elemental and SO 4 2- 、NO 3 - , salts, non-toxic and harmless gases and other substances are discharged. It is a high-efficiency deodorizing bacteria that can be applied to the field of biological deodorization and environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a colony morphology diagram of Pseudomonas in an embodiment of the present invention;

[0024] Figure 2 A phylogenetic tree diagram of the Pseudomonas of the present invention and its 10 most closely related strains;

[0025] Figure 3This is a 24-hour growth curve of Pseudomonas in LB according to an embodiment of the present invention;

[0026] Figure 4 The number of live Pseudomonas at different temperatures in the embodiment of the present invention;

[0027] Figure 5 is the number of live bacteria of Pseudomonas in different pH conditions in the embodiment of the present invention;

[0028] Figure 6 is a growth curve of Pseudomonas in an embodiment of the present invention with sodium hypochlorite in a concentration range of 0-5 mg / L;

[0029] Figure 7 This is a graph showing the removal rate of ammonia by Pseudomonas in an embodiment of the present invention in a laboratory;

[0030] Figure 8 This is a graph showing the removal rate of hydrogen sulfide by Pseudomonas in an embodiment of the present invention in a laboratory;

[0031] Fig. 9 is a graph showing the removal rate of VOCs by Pseudomonas in an embodiment of the present invention in a biofilter column device;

[0032] Fig.10 This is a graph showing the removal rate of VOCs by Pseudomonas according to an embodiment of the present invention in a biological filter. DETAILED DESCRIPTION

[0033] The present invention will be described clearly and completely below in conjunction with specific embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] The present invention adopts the removal rate of ammonia, hydrogen sulfide and VOCs generated in food waste by Pseudomonas with a preservation number of CGMCC No. 30345 as an evaluation standard.

[0035] Example 1

[0036] This example provides screening and identification of Pseudomonas:

[0037] 1. Cultivation and initial screening:

[0038] Twenty different strains were initially screened from the kitchen waste samples of a restaurant, with NH 3 , H 2 S and VOCs degradation activity were used as the evaluation criteria. Ammonia and sulfur-containing enrichment medium were used for preliminary screening, and two strains with strong deodorization activity were purified using ammonia and sulfur-containing purification medium.3 , H 2 The S degradation efficiency and growth ability of the strain were screened again, and a highly efficient Pseudomonas sp. was obtained, which the applicant named strain RTL-08.

[0039] Luria-Bertani medium (LB): 10.0 g tryptone, 10.0 g sodium chloride (NaCl), 5.0 g yeast extract, 15.0 g agar powder, add distilled water to 1000 mL, pH = 7.4, sterilize at 121°C for 20 min.

[0040] Beef extract peptone medium (NA): 3.0 g beef extract, 5.0 g sodium chloride (NaCl), 10.0 g peptone, 15.0 g agar powder, add distilled water to 1000 mL, pH = 7.5 ± 0.1, sterilize at 121 ° C for 20 min.

[0041] Ammonia-enriched medium: sucrose 5.0 g, peptone 2.0 g, potassium dihydrogen phosphate (KH 2 PO 4 ) 2.0 g, magnesium sulfate (MgSO 4 7H 2 O) 0.5 g, sodium chloride (NaCl) 2.0 g, zinc sulfate (ZnSO 4 ) 0.05 g, ferrous sulfate (FeSO 4 ) 0.04g, add distilled water to 1000mL, pH=7.0, sterilize at 121℃ for 20min, add 5.0mL of filtered sterilized ammonia water after sterilization; add 15.0g of agar powder during coating and purification.

[0042] Sulfur-enriched medium: ammonium chloride (NH 4 Cl) 2.0 g, magnesium chloride (MgCl 2 ) 0.5g, potassium dihydrogen phosphate (K 2 HPO 4 ·3H 2 O) 3.0 g, sodium sulfide (Na 2 S·9H 2 O) 10.0 g (filter sterilized, add after sterilization), calcium chloride (CaCl 2 6H 2 O) 0.2g, agar powder 15.0g, add distilled water to 1000mL, pH = 6.1±0.1, sterilize at 121℃ for 20min.

[0043] Ammonia-containing purified medium: trisodium citrate (C 6 H 5 Na 3 O 7 ·2H2 O) 5.0 g, ammonium sulfate ((NH 4 ) 2 SO 4 ) 0.45g, potassium dihydrogen phosphate (K 2 HPO 4 ) 0.25 g, ferrous sulfate (FeSO 4 7H 2 O) 0.0025 g, sodium chloride (NaCl) 0.125 g, magnesium sulfate (MgSO 4 7H 2 O) 0.06 g, manganese sulfate (MnSO 4 ·H 2 O) 0.001g, agar powder 15.0g, add distilled water to 1000mL, pH = 7.0, sterilize at 121℃ for 20min.

[0044] Sulfur purification medium: ammonium chloride (NH 4 Cl) 2.0 g, magnesium chloride (MgCl 2 ) 0.5g, potassium dihydrogen phosphate (K 2 HPO 4 ·3H 2 O) 3.0 g, sodium thiosulfate (Na 2 S 2 O 3 ·5H 2 O) 10.0 g, calcium chloride (CaCl 2 6H 2 O) 0.2g, agar powder 15.0g, add distilled water to 1000mL, pH = 6.1±0.1, sterilize at 121℃ for 20min.

[0045] Sulfide degradation medium: ammonium chloride (NH 4 Cl) 0.4 g, magnesium chloride (MgCl 2 ) 0.2g, potassium dihydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0g, sodium carbonate (Na 2 CO 3 ) 0.4g, add distilled water to 1000mL, pH = 6.1 ± 0.1, sterilize at 121 ° C for 20 min; sodium sulfide (Na 2 S·9H 2 O) 0.1 g was dissolved and filtered through a 0.22 μm filter before addition.

[0046] Weigh 10g of the collected fresh kitchen waste sample, inoculate it into a sterilized triangular flask containing 25mL of sterile water, add 10 sterilized glass beads, and shake and mix at 30℃ and 200r / min for 1h to release the bacteria into the water phase. Take 5mL of the shaken culture medium and transfer it to 100mL of ammonia or sulfur-containing enrichment medium, and continue to culture at 30℃ and 200r / min; when the culture medium becomes turbid, take 5mL of the turbid sample and transfer it to 100mL of the same enrichment medium again to gradually enrich the candidate bacteria again, and finally obtain the final culture medium of the two culture media.

[0047] The two final culture solutions were alternately plated on ammonia-containing purified medium and sulfur-containing purified medium plates to separate and screen functional bacteria. Specifically, the original culture solution was first diluted to 10 -4 , 10 -5 , 10 -6 Gradient, take 100 μL of different gradient dilutions and evenly spread them on agar plates containing ammonia or sulfur-containing purified medium, and place the plates in a 30°C incubator for culture. When the colonies grow out, pick out single colonies with different shapes, sizes and appearances, and use the three-zone streak method to streak and culture them in a purified medium different from the last time. For example, when the first application is ammonia-containing purified medium, the single colonies picked out will be streaked and cultured on sulfur-containing purified medium this time. In this way, alternate streaking and culturing single colonies on different purified mediums is repeated twice, and the final result on the two plates is the primary screening purified strain.

[0048] 2. Rescreening of deamination strains:

[0049] The rescreening of deamination strains is to use Nessler's colorimetric method to select strains with strong deamination ability.

[0050] The strain to be tested is the purified strain with degradation activity that was initially screened in the above two plates. Single colonies of each strain were picked and transferred to beef extract peptone liquid medium (NA) at 30℃ and 200r / min overnight culture to obtain fresh seed liquid. The seed liquid was transferred to heterotrophic nitrification medium, beef extract peptone medium (NA) at an inoculation rate of 5%, placed in a constant temperature shaker at 30℃ and 200r / min for 24h, and then centrifuged in a centrifuge at 12000 r / min for 2min. The supernatant was taken and the color was developed using Nessler's reagent colorimetric method. The medium without bacteria (CK) was used as a negative control and the ammonia-free water was used as a blank control. The absorbance value was determined using an enzyme marker at a wavelength of 420 nm. Three replicates were set for each treatment. Using ammonia-free water as a reference and referring to the absorbance value of the standard curve, the sample was compared with CK, and the residual ammonia nitrogen content in the supernatant was calculated to obtain the ammonia nitrogen removal rate in the culture medium. By comparing the ammonia nitrogen removal rates of the purified strains, the efficient deammonification strains were selected for the next experiment. When using the Nessler's reagent colorimetric method, 1.0 mL of the supernatant after centrifugation of the bacterial solution was added with 0.2 mL of potassium sodium tartrate solution and mixed on an oscillator. Then 0.3 mL of Nessler's reagent solution was added, and ammonia-free water was added to make up to the mark of 10 mL. After sufficient mixing, the mixture was allowed to stand for 10 min, and the absorbance was measured at a wavelength of 420 nm.

[0051] The absorbance of different known concentrations of ammonium standard solutions was measured using Nessler colorimetry, and a standard curve was drawn with ammonia nitrogen content (mg) versus corrected absorbance. Specifically, 0 mL, 0.10 mL, 0.20 mL, 0.60 mL, 1.00 mL, and 2.00 mL of 0.01 mg / mL ammonium standard solution were successively added with 0.2 mL of potassium sodium tartrate solution and 0.3 mL of Nessler's reagent, mixed and added to 5 mL, and allowed to stand for 10 min. The absorbance was measured at a wavelength of 420 nm, and the absorbance of the blank tube without ammonia water was subtracted to obtain the calibration absorbance value, and a standard curve was drawn.

[0052] 3. Rescreening of desulfurization strains:

[0053] The rescreening of desulfurization strains is to use methylene blue colorimetry to screen strains with strong desulfurization ability.

[0054] The strains to be tested were also purified strains with degradation activity that were initially screened in two plates. Single colonies of each strain were picked and transferred to beef extract peptone liquid medium (NA) at 30°C and 200r / min for overnight culture to obtain fresh seed liquid. The seed liquid was transferred to sodium sulfide degradation medium at an inoculation rate of 5%, placed in a constant temperature shaker at 30°C and 200r / min for 24h, and then centrifuged in a centrifuge at 12000r / min for 2min. The supernatant was taken and the color was developed using the methylene blue colorimetric method. The medium without bacteria (CK) was used as a negative control and carbon dioxide-free water was used as a blank control. The absorbance value was determined using an enzyme marker at a wavelength of 665 nm, and 3 replicates were set for each treatment. Using carbon dioxide-free water as a reference and referring to the absorbance value of the standard curve, the sample was compared with CK, and the residual sulfide content in the supernatant was calculated to obtain the sulfide removal rate in the culture medium. By comparing the sulfide removal rates of each purified strain, a highly efficient desulfurization strain was selected for the next experiment.

[0055] When developing the color by the methylene blue colorimetric method, take 1.0 mL of the supernatant after centrifugation of the bacterial solution and add 1.0 mL of p-aminodimethylaniline solution, slowly invert it until mixed. This process should not be performed violently. Then add 0.2 mL of ammonium ferric sulfate solution, add carbon dioxide-free water to make up to 5 mL, mix thoroughly, let it stand for 10 minutes, and measure the absorbance at a wavelength of 420 nm.

[0056] The absorbance of sulfur standard solutions of different known concentrations was measured by methylene blue colorimetry, and a standard curve was drawn with sulfide content (ug) versus corrected absorbance. Specifically, 1 mL of sulfur standard solution of 0, 0.10, 0.50, 1.00, 5.00, and 10.00 ug / mL was taken, and 1.0 mL of p-aminodimethylaniline solution and 0.2 mL of ammonium ferric sulfate solution were added in sequence, and then the solution was filled to 5 mL and allowed to stand for 10 minutes. The absorbance was measured at a wavelength of 420 nm, and the absorbance of the blank tube without ammonia water was subtracted to obtain the calibration absorbance value, and a standard curve was drawn.

[0057] Finally, by comparing the effects of different strains in desulfurization and deammoniation culture media, a strain RTL-08 with strong desulfurization and denitrification capabilities was obtained.

[0058] 4. Strain identification and basic property testing:

[0059] 4.1 Strain morphological structure observation and physiological and biochemical tests

[0060] Depend on Figure 1It can be seen that the single colony has a growth diameter of 0.1-0.5cm, and is an opaque, plump, small round milky white protrusion with neat and clear edges, a smooth and moist surface, and the colony is easy to pick up; the results of the contact enzyme test and the oxidase reaction are both positive; it can decompose glucose; it can decompose lactose to produce alkaline metabolites; it has no gelatin liquefaction ability and starch decomposition ability; it is Gram-negative.

[0061] The strain was subjected to corresponding biochemical tests, and the results are shown in Table 1.

[0062] Table 1: Biochemical test results

[0063] Biochemical Project Result (+: positive; -: negative) Gram staining - Catalase assay + Oxidase detection + Starch hydrolysis ability test - Gelatin liquefaction ability test - Glucose fermentation capacity test + Milk fermentation capacity test +; can decompose lactose and produce alkaline metabolites

[0064] 4.2 Sequence analysis of strain 16S rDNA

[0065] The purified single colony of strain RTL-08 was subjected to colony PCR based on 16SrDNA and sequenced. Specifically, a small amount of single colony of strain RTL-08 was picked and dissolved in 10μL sterile water as the mother bacterial solution. After the mother bacterial solution was diluted 5 times, 20μL of the diluted bacterial solution was taken for cell lysis at 98°C for 10 minutes to obtain the lysate as the template DNA. The template DNA was amplified based on the front and back primers of the universal primer 27F / 1522R, and sequenced. The sequence result is shown in SEQ ID NO:1; the 16SrDNA sequence measured by the strain RTL-08 was compared with BLASTn on the NCBI website, and the top ten bacterial sequences with similarity were downloaded to build a phylogenetic tree together (the step value was set to 1000) as shown Figure 2 As shown, the strain RTL-08 was found to be similar to Pseudomonas guajava Pseudomonas guariconensis strain PCAVU11 (NCBI number: NR_135703.1) has the closest relationship (confidence 98%), so this strain is determined to be Pseudomonas guarana Pseudomonas guariconensis It is a member of the genus and is classified and named Pseudomonas sp. It was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on April 15, 2024, with the deposit number: CGMCC No.30345. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code: 100101. The single colony of this strain has a diameter of about 0.1-0.5 cm, and is an opaque, plump, small round milky white protrusion with neat and clear edges, a smooth and moist surface, and the colony is easy to pick up. The colony morphology is shown in the figure below. Figure 1 shown.

[0066] 5. Strain stability test:

[0067] The strain RTL-08 can be stably grown in LB medium. Specifically, the strain RTL-08 mother bacteria (OD 600 =0.5) was inoculated into LB liquid culture medium at 1% inoculum, and the growth curve was tracked for 24 hours at 30°C using an ELISA reader. The strain RTL-08 entered the logarithmic phase at about the 4th hour and entered the plateau phase at the 10th to 12th hour, reaching the maximum growth concentration at this time, OD 600 =0.8, then the number of viable bacteria is about 1.6*10 10 CFU / mL, the strain adaptation period is short, and its growth curve is as follows Figure 3 As shown, the strain grows normally under the conditions of 20-50°C, pH 3-11, and sodium hypochlorite 0-5 mg / L, as follows:

[0068] The RTL-08 mother bacteria (OD 600 =0.5) were inoculated into LB liquid culture medium at different temperatures (20, 30, 40, 50°C) at a 1% inoculum amount, and samples were taken at 6 and 12 hours to detect the absorbance at 600 nm. The strain RTL-08 could grow to 10 within 12 hours at 20-50°C. 9 CFU / mL, indicating that strain RTL-08 can adapt well to a wide range of temperature environments. The growth results at different temperatures are as follows Figure 4 shown.

[0069] The RTL-08 mother bacteria (OD 600 =0.5) was inoculated into LB liquid culture medium with different pH values ​​(3.0-11.0) at 1% inoculum. Samples were taken at 6, 12, 18, and 24 hours to detect absorbance at 600 nm. Within 12 hours, strain RTL-08 could grow rapidly in a culture environment with a pH value of 4-10; within 18 hours, strain RTL-08 could grow in a culture environment with a pH value of 4-11, indicating that strain RTL-08 could adapt well to a wide range of pH values. The growth results at different pH values ​​are shown in Figure 2. Figure 5 shown.

[0070] The RTL-08 mother bacteria (OD 600 =0.5) were inoculated at 1% inoculum into LB liquid medium with different concentrations of sodium hypochlorite (0, 1.0, 2.5, 5 mg / L), and the growth curve was monitored at 30°C for 24 hours. Although the highest concentration of 5 mg / L sodium hypochlorite reduced the viable count of strain RTL-08 by 6.85±0.56% (in terms of CFU), the addition of sodium hypochlorite did not delay the time for the strain to reach the logarithmic phase and the plateau phase, that is, sodium hypochlorite in the concentration range of 0-5 mg / L had no significant effect on the growth of strain RTL-08. The growth results of different concentrations of sodium hypochlorite are shown in Figure 2. Figure 6 shown.

[0071] Example 2

[0072] This embodiment provides an application of strain RTL-08, and the strain RTL-08 is used for biological deodorization in the laboratory.

[0073] Nessler's reagent colorimetric method was used in the laboratory to evaluate the ammonia removal rate of strain RTL-08. 600 =0.5) was inoculated into a heterotrophic nitrification medium, namely beef extract peptone medium (NA), at a 5% inoculum amount, and cultured in a constant temperature shaker at 30°C and 200r / min. Samples were taken at 6, 12, and 24 hours, and the supernatant was taken for colorimetric development using Nessler's reagent. The remaining ammonia nitrogen content in the supernatant was calculated to obtain the ammonia nitrogen removal rate in the culture medium at different times. Three replicates were set for each treatment. The strain RTL-08 could achieve a deammoniation rate of 23.6±0.9% in the 6th hour, and a 96.0±0.03% ammonia removal effect in the 24th hour. The results are shown in Figure 7 shown.

[0074] The methylene blue colorimetric method was used in the laboratory to evaluate the removal rate of hydrogen sulfide by strain 1. 600 =0.5) was inoculated into sodium sulfide degradation medium at 5% inoculum, and cultured in a constant temperature shaker at 30°C and 200r / min. Samples were taken at 6, 12, 24, 36 and 48h, and the supernatant was used for colorimetric analysis using methylene blue. The remaining sulfur content in the supernatant was calculated to obtain the sulfide removal rate in the culture medium at different times. Three replicates were set for each treatment. The strain RTL-08 could achieve a desulfurization rate of 17.2±2.3% at the 12th hour, and a desulfurization effect of 90.1±3.6% at the 48th hour. The results are shown in Figure 8 shown.

[0075] Example 3

[0076] This embodiment provides an application of strain RTL-08, and the strain RTL-08 is used for biological deodorization under a biological filter column.

[0077] The deodorized waste gas sample is the malodorous waste gas from the food waste pretreatment unit of a bioenergy company. By adding strain RTL-08, four removal experiments were conducted at different times in June and July 2023, and the removal rate was calculated by measuring the content of VOCs in the inlet and outlet air. The specific process is:

[0078] (1) Add the RTL-08 bacterial solution (the number of viable cells is about 10 9 CFU / mL, the inoculation amount is 2‰) is added into the biofilter column and stirred evenly, and the strain forms a biofilm on the filler inside the biofilter column;

[0079] (2) After the membrane is completed, removal experiments will be carried out in June and July 2023. The odor generated during the garbage treatment process will be introduced through a fan. The average VOCs inlet concentration is 80 ppm. The odor will be decomposed into CO under the action of the strain. 2 , water and other harmless substances, and then discharged.

[0080] The average removal rate of VOCs by strain RTL-08 is 95.9%. Fig. 9 shown.

[0081] Example 4

[0082] The strain RTL-08 is mainly used for deodorizing food waste, but it is also effective in removing odors from other wastes. This embodiment provides an application of the strain RTL-08, and the strain RTL-08 is used for biological deodorization in a biological filter column pool.

[0083] The deodorized waste gas sample is the malodorous waste gas generated by a certain Environmental Protection Technology Co., Ltd. After adding strain RTL-08 to the biological filter, 8 removal rate measurements were carried out at different times from July to October 2023. The removal rate was calculated by measuring the content of VOCs in the inlet and outlet air.

[0084] The specific process is:

[0085] (1) Add the RTL-08 bacterial solution (the number of viable cells is about 10 9 CFU / mL, inoculation amount is 2‰) added to the biofilter and stirred evenly, waiting for the strain to stabilize for 10 days, and the strain formed biofilm on the filler inside the biofilter;

[0086] (2) After the membrane is completed, a removal experiment will be carried out from July to October 2023. The odor generated during the garbage treatment process will be introduced through a fan. The average VOCs inlet concentration is 76ppm. The odor will be decomposed into CO under the action of the strain. 2 , water and other harmless substances, and then discharged.

[0087] The average removal rate of VOCs by strain RTL-08 is 92.0%. Fig.10 shown.

[0088] The present invention separates and screens the superior strain RTL-08 which can effectively remove hydrogen sulfide, ammonia and other VOCs from the food waste samples. According to the test, the strain can grow rapidly to the maximum effective viable count within 12 hours at 30°C, which can reach 10 10CFU / mL, and can grow normally under the conditions of pH4-11 and sodium hypochlorite 0-5mg / L. The strain can remove 96.03% of ammonia and 94.7% of hydrogen sulfide. The strain has high vitality and strong adaptability, and can remove hydrogen sulfide and ammonia under a wide range of pH conditions; after being added to the biofilter column or biofilter, it also has a stable removal effect on volatile organic compounds (VOCs), and is an efficient deodorizing strain that can be applied to the field of biological deodorization and environmental protection.

[0089] In addition to the above embodiments, the present invention may also have other implementation modes. Any technical solution formed by equivalent replacement or equivalent transformation falls within the protection scope required by the present invention.

Claims

1. A Pseudomonas sp., characterized in that: Pseudomonas sp. Pseudomonas sp., deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on April 15, 2024, with the deposit number: CGMCCNo.30345; The strain grew to the maximum effective viable count of 10 within 12 hours at 30°C. 10 CFU / mL; and grow normally under the conditions of 20-50℃, pH4-11, and sodium hypochlorite 0-5 mg / L; The 16S rRNA gene sequence of the Pseudomonas is shown as SEQ ID NO.

1.

2. A bacterial agent containing the Pseudomonas as claimed in claim 1.

3. Use of the Pseudomonas as claimed in claim 1 in deodorizing food waste.

4. The use of Pseudomonas according to claim 3 in deodorizing food waste, characterized in that: Under laboratory conditions, the Pseudomonas or bacterial agents containing the Pseudomonas can remove more than 95% of ammonia within 24 hours, and more than 86% of hydrogen sulfide within 48 hours.

5. The use of Pseudomonas in deodorizing food waste according to claim 3, characterized in that: The specific steps include: (1) Inoculating Pseudomonas or a bacterial agent containing the Pseudomonas into a bioreactor, and allowing the strain to form a biofilm on the filler inside the bioreactor; (2) After the membrane is completed, the odor generated during the treatment of food waste is introduced through a fan, and the odor is decomposed and discharged under the action of the bacterial strain.

6. The use of Pseudomonas in deodorizing food waste according to claim 5, characterized in that: The bioreactor is a biofilter column, and the average VOCs removal rate reaches 95.9%.

7. The use of Pseudomonas in deodorizing food waste according to claim 5, characterized in that: The bioreactor is a biofilter, and the average VOCs removal rate reaches 92.0%.

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