Monomolecular LB film groove hanging instrument and monomolecular LB film preparation method

Abstract

The invention belongs to the technical field of LB membrane hanging instruments, and particularly relates to a single-molecule LB membrane groove hanging instrument and a single-molecule LB membrane preparation method, and the single-molecule LB membrane hanging instrument comprises a membrane forming structure which comprises a liquid groove and is used for containing a stock solution and generating a single-molecule LB membrane in a compression mode; the leveling device is arranged at the bottom of the liquid tank and is used for adjusting the level of the liquid level in the liquid tank; the detection device is arranged on one side of the liquid tank and detects the tension of the film in the film forming process through a tension sensor; and the biofilm culturing device is arranged on the other side of the liquid tank, an electrode or a field-effect tube substrate to be subjected to biofilm culturing is fixed on a biofilm culturing clamping seat, and a monomolecular film is vertically and slowly lifted and hung. The method mainly aims at a monomolecular layer or 2-3 layers of molecular films, is compact and simple in design structure, is easy to operate, and greatly reduces the manufacturing cost. The preparation method provided by the invention is simple to operate and can meet the biofilm culturing requirements of the gold interdigital and the field-effect tube.

Description

Technical Field

[0001] The invention belongs to the technical field of LB film hanging apparatus, in particular to a single molecule LB film trough hanging apparatus and a single molecule LB film preparation method. Background Art

[0002] The LB (Langmuir-Blodgett) monolayer film preparation and characterization equipment is a world-leading equipment with the widest application in the field of LB film deposition. The LB film analyzer is equipped with a liquid tank and a film head. At the required stacking density, the film head can be used to transfer the Langmuir film to the solid substrate. The film head can transfer the Langmuir film to the sample under the Langmuir film tank. The properties such as density, thickness and uniformity will be retained, thus realizing the possibility of preparing multi-molecular layer structures of different compositions. Compared with other organic thin film deposition techniques, the LB deposition method is less affected by the molecular structure restrictions of functional molecules, which means that the LB technology is the only bottom-up assembly method.

[0003] The existing monomolecular film hanging instrument has the problems of complex structure, high cost and complex control. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a monomolecular LB film trough hanging instrument and a monomolecular LB film preparation method, so as to solve the problems of complex structure, high cost and complex control of the existing monomolecular film hanging instrument.

[0005] The present invention is achieved in this way.

[0006] A single molecule LB film trough hanging instrument, comprising:

[0007] The film-forming structure includes a liquid tank for containing the original solution and forming a single-molecule LB film by compression;

[0008] A leveling device, arranged at the bottom of the liquid tank, for adjusting the level of the liquid surface in the liquid tank;

[0009] The detection device is arranged on one side of the liquid tank and detects the tension of the mold during the film forming process through a tension sensor;

[0010] The film hanging device is arranged on the other side of the liquid tank, fixes the electrode or field effect tube substrate to be hung on the film hanging holder, and slowly pulls up and down to hang the monomolecular film.

[0011] Furthermore, the film forming structure includes a liquid tank, a low-frequency one-way slide located above the liquid tank and capable of moving toward each other, and a first stepper motor that drives the low-frequency one-way slide to move through a screw rod.

[0012] Furthermore, the leveling device comprises an adjustment support column located at the bottom of the liquid tank, the other end of the adjustment support column is fixed to the flat plate on the bottom surface, and the adjustment support column is sleeved with an adjustment bolt.

[0013] Furthermore, the detection device includes a first column whose up and down strokes can be controlled by a screw guide rail structure, the first column has a shell, a guide rail is arranged on the inner side of the shell, the screw is connected to the first column, the other end of the screw is connected to the second stepper motor, and a tension sensor and a temperature sensor are vertically arranged at the bottom end of the first column. The detection device includes a second column connected to the side of the shell of the first column, a slider is arranged at the bottom of the second column, and the slider is mounted on a track with balls on both sides.

[0014] Furthermore, the film hanging device includes a third column whose up and down strokes can be controlled by a screw guide rail structure, a screw is passed through the third column, one end of the screw is connected to a third stepping motor, and a guide rail is arranged on the side of the third column, and a part to be hung is vertically arranged at the bottom end of the third column. The film hanging device includes a fourth column connected to the side of the third column, a slider is arranged at the bottom of the fourth column, and the slider is mounted on a track with ball bearings on both sides.

[0015] Furthermore, a film hanging holder is provided at the bottom end of the third column for fixing the parts to be hung.

[0016] A method for preparing a monomolecular LB film, comprising:

[0017] Deionized water, a surfactant, a buffer and a monomolecular film substance are sequentially added into the liquid tank;

[0018] Adjust the liquid level;

[0019] Start the first stepper motor to control the low-frequency one-way slides at both ends to slowly move from both ends of the liquid tank to the middle of the liquid tank to push the liquid. When it moves from both ends to the middle, manually remove the low-frequency one-way slide and wait for the slider of the first motor to move to both ends again, then install the low-frequency one-way slide on the slider, and then move to the middle, repeating several times;

[0020] Manually push the second column on the track until the tension sensor and the temperature sensor are located in the middle of the liquid tank;

[0021] Start the second stepper motor and repeatedly move the tension sensor up and down to detect the surface tension value of the liquid;

[0022] When the surface tension reaches the minimum value and no longer changes, push the film hanging device to the middle of the liquid tank; slowly pull it up and down to hang the monomolecular film.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention is mainly aimed at a single molecular layer or a 2-3-layer molecular film, and is compact and simple in design structure, easy to operate, and greatly reduces the production cost. The preparation method provided is simple to operate and meets the film requirements of gold interdigitators and field effect tubes. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A schematic diagram of the structure of a film forming structure and a leveling device of a single molecule LB film trough hanging instrument provided in an embodiment of the present invention;

[0026] Figure 2 A schematic diagram of a liquid injected into a liquid tank of a single-molecule LB film tank hanging instrument provided by an embodiment of the present invention;

[0027] Figure 3 A schematic diagram of the structure of a detection device and a film-forming device provided by an embodiment of the present invention;

[0028] Figure 4 A partial structural schematic diagram of a film forming structure provided by an embodiment of the present invention is provided. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0030] The film-forming principle on which the present invention is based is that the monolayer formed by insoluble functional molecules, nanoparticles, nanowires or microparticles at the gas-liquid or liquid-liquid interface can be defined as a Langmuir film. These molecules can move freely at the interface, have strong fluidity, and are easy to control their packing density to study the behavior of the monolayer. A Langmuir film can be obtained by depositing the material on the water subphase in a shallow pool. Under the action of a low-frequency unidirectional slide, the monolayer can be compressed. The surface tension, i.e., the packing density, can be detected and controlled by a tension sensor in a Langmuir film tank control system.

[0031] In a typical isothermal compression test, the monolayer first changes from a two-dimensional gas phase (G) to a liquid phase (L) and finally forms an ordered solid phase (S). In the gas phase, the interaction between molecules is relatively weak; when the surface area is reduced, the molecules are packed more tightly and begin to interact.

[0032] The LB film deposition process is to pull the sample vertically out of the monolayer. Multilayer LB films can be prepared through repeated deposition technology. Both hydrophilic and hydrophobic samples can be deposited as a monolayer in the liquid or gas phase.

[0033] See also Figure 1 Combination Figure 2-Figure 4 As shown, a single molecule LB film trough hanging instrument comprises:

[0034] The film-forming structure includes a liquid tank for containing the original solution and forming a single-molecule LB film by compression;

[0035] A leveling device, arranged at the bottom of the liquid tank, for adjusting the level of the liquid surface in the liquid tank;

[0036] The detection device is arranged on one side of the liquid tank and detects the tension of the mold during the film forming process through a tension sensor;

[0037] The film hanging device is arranged on the other side of the liquid tank, fixes the electrode or field effect tube substrate to be hung on the film hanging holder, and slowly pulls up and down to hang the monomolecular film.

[0038] In this embodiment, the film forming structure includes a liquid tank 5, a low-frequency one-way slide 9 located above the liquid tank and capable of moving toward each other, and a first stepper motor 7 that drives the low-frequency one-way slide to move through a screw. The liquid tank is a rectangular structure, and there are two low-frequency one-way slides. A screw is provided on one side of the liquid tank. The low-frequency one-way slide is installed on the screw through the first slider 8. The two sides of the center of the screw are threads in different directions. When driven by the first stepper motor, the two low-frequency one-way slides move forward in different directions. As a result, the low-frequency one-way slides move toward each other and compress the monolayer.

[0039] In this embodiment, the leveling device includes an adjustment support 3 located at the bottom of the liquid tank, a support frame 4 is provided at the bottom of the liquid tank, the adjustment support 3 is installed on the support frame 4, the other end of the adjustment support 3 is fixed to the bottom plate 1, and the adjustment support is sleeved with an adjustment bolt 2. The level of the liquid surface in the liquid tank is adjusted by the adjustment bolt.

[0040] The detection device includes a first column 68 that realizes controllable up and down strokes through a screw guide rail structure. The first column has a housing, a guide rail is arranged inside the housing, a screw is connected to the first column, the other end of the screw is connected to a second stepping motor, a tension sensor 67 and a temperature sensor 66 are vertically arranged at the bottom end of the first column, the detection device includes a second column connected to the side of the housing of the first column, a second slider is arranged at the bottom of the second column, and the second slider 61 is sleeved on a track with balls on both sides. The tension sensor 67, the temperature sensor 66 and the control line 69 of the second stepping motor pass through the housing of the first column and are connected to an external controller.

[0041] The film hanging device includes a third column 64 that realizes controllable up and down travel through a screw guide rail structure, the third column 64 has a shell, a guide rail is arranged inside the shell, a screw is connected to the third column, the other end of the screw is connected to a third stepping motor, a to-be-hanged part is vertically arranged at the bottom end of the third column, a fourth column is connected to the side of the shell of the third column, a slider is arranged at the bottom of the fourth column, the slider is sleeved on a track with balls on both sides, and during operation, the film hanging device is manually adjusted to a suitable position along the track. A film hanging holder 63 is arranged at the bottom end of the third column for fixing the to-be-hanged part 62, and a control line 65 of the third stepping motor passes through the shell of the third column and is connected to an external controller.

[0042] The present invention provides a method for preparing a monomolecular LB film, comprising:

[0043] Deionized water 51, surfactant 52, buffer 53 and monomolecular film material 54 are sequentially added into the liquid tank;

[0044] Adjust the liquid level;

[0045] Start the first stepper motor to control the low-frequency one-way slides at both ends to slowly move from both ends of the liquid tank to the middle of the liquid tank to push the liquid. When it moves from both ends to the middle, manually remove the low-frequency one-way slide and wait for the slider of the first motor to move to both ends again, then install the low-frequency one-way slide on the slider, and then move to the middle, repeating several times;

[0046] Manually push the second column on the track until the tension sensor and the temperature sensor are located in the middle of the liquid tank;

[0047] Start the second stepper motor and repeatedly move the tension sensor up and down to detect the surface tension value of the liquid;

[0048] When the surface tension reaches the minimum value and no longer changes, push the film hanging device to the middle of the liquid tank; start the third stepper motor to slowly pull up and down to hang the monomolecular film.

[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A single molecule LB film trough hanging instrument, characterized in that: include: The film-forming structure includes a liquid tank for containing the original solution and forming a monomolecular LB film by compression; A leveling device, arranged at the bottom of the liquid tank, for adjusting the level of the liquid surface in the liquid tank; The detection device is arranged on one side of the liquid tank and detects the tension of the mold during the film forming process through a tension sensor; The film hanging device is arranged on the other side of the liquid tank, fixes the electrode or field effect tube substrate to be hung on the film hanging holder, and slowly pulls up and down to hang the monomolecular film.

2. The monomolecular LB film trough hanging instrument according to claim 1, characterized in that: The film forming structure comprises a liquid tank, a low-frequency one-way slide located above the liquid tank and capable of moving toward each other, and a first stepper motor for driving the low-frequency one-way slide to move through a lead screw.

3. The monomolecular LB film trough hanging instrument according to claim 1, characterized in that: The leveling device comprises an adjusting support column located at the bottom of the liquid tank, the other end of the adjusting support column is fixed to the flat plate on the bottom surface, and the adjusting support column is sleeved with an adjusting bolt.

4. The monomolecular LB film trough hanging instrument according to claim 1, characterized in that: The detection device includes a first column whose up and down strokes can be controlled by a screw guide rail structure. The first column has a shell, a guide rail is arranged on the inner side of the shell, the screw is connected to the first column, and the other end of the screw is connected to the second stepping motor. A tension sensor and a temperature sensor are vertically arranged at the bottom end of the first column. The detection device includes a second column connected to the side of the shell of the first column, a slider is arranged at the bottom of the second column, and the slider is mounted on a track with balls on both sides.

5. The monomolecular LB film trough hanging instrument according to claim 4, characterized in that: The film hanging device includes a third column whose up and down strokes are controllable through a screw guide rail structure. The film hanging device includes a third column whose up and down strokes are controllable through a screw guide rail structure. The third column has a shell, a guide rail is arranged inside the shell, a screw is connected to the third column, the other end of the screw is connected to a third stepping motor, a part to be hung is vertically arranged at the bottom end of the third column, and a fourth column is connected to the side of the shell of the third column. A slider is arranged at the bottom of the fourth column, and the slider is sleeved on a track with ball bearings on both sides.

6. The monomolecular LB film trough hanging instrument according to claim 5, characterized in that: A film hanging holder is arranged at the bottom end of the third column for fixing the parts to be hung.

7. A method for preparing a monomolecular LB film, using the monomolecular LB film trough hanging apparatus according to any one of claims 1 to 6, characterized in that: include: Deionized water, a surfactant, a buffer and a monomolecular film substance are sequentially added into the liquid tank; Adjust the liquid level; Start the first stepper motor to control the low-frequency one-way slides at both ends to slowly move from both ends of the liquid tank to the middle of the liquid tank to push the liquid. When it moves from both ends to the middle, manually remove the low-frequency one-way slide and wait for the slider of the first motor to move to both ends again, then install the low-frequency one-way slide on the slider, and then move to the middle, repeating several times; Manually push the second column on the track until the tension sensor and the temperature sensor are located in the middle of the liquid tank; Start the second stepper motor and repeatedly move the tension sensor up and down to detect the surface tension value of the liquid; When the surface tension reaches the minimum value and no longer changes, push the film hanging device to the middle of the liquid tank; slowly pull it up and down to hang the monomolecular film.

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